Chemiluminescence assay of inflammation
Номер патента: AU6644390A
Опубликовано: 31-05-1991
Автор(ы): Robert Charles Allen
Принадлежит: Exoxemis Inc
Опубликовано: 31-05-1991
Автор(ы): Robert Charles Allen
Принадлежит: Exoxemis Inc
Реферат: The presence or amount of inflammation of a patient is determined by comparing the extent of opsonin receptor expression on phagocytes of a patient with the maximum opsonin receptor expression inducible on phagocytes of the patient in vitro after stimulation with a receptor expression priming agent. Preferably, the state of inflammation of a patient is determined by contacting a first portion of a phagocyte containing biological sample from the patient with an opsonified oxidative metabolism stimulating agent capable of eliciting metabolic activation and with a chemiluminigenic substrate, contacting a second portion of the biological sample from the patient with an opsonin receptor expression priming agent, an opsonified oxidative metabolism stimulating agent capable of eliciting metabolic activation and a chemiluminigenic substrate, and then comparing the chemiluminescence response of the first and second portions of the sample as a measure of the immune response potential or state of inflammation of the patient. Phagocyte function is additionally quantitatively evaluated by measuring the phagocyte oxygenation capacity of a maximally opsonin receptor primed and stimulated biological sample of a patient, determining the specific oxygenation capacity to a set of controls representing the normal distribution of specific oxygenation capacity established from testing a large population. The phagocyte-specific oxygenation capacity is determined by contacting the sample with an opsonin receptor expression priming agent, an opsonified oxidative metabolism stimulating agent and a chemiluminigenic substrate, measuring the chemiluminescence response of the sample, determining the chemiluminescence response per phagocyte of the sample and comparing the response per phagocyte with that of the normal range of values. Kits and reagents are provided for use in the practice of the disclosed methods.
Chemiluminescence assay of in vivo inflammation
Номер патента: CA2028605A1. Автор: Robert Charles Allen. Владелец: Robert Charles Allen. Дата публикации: 1991-05-01.