Process for the expression of foreign genes in yeasts
Номер патента: CA2031810A1
Опубликовано: 09-06-1991
Автор(ы): Mathias Grote, Michael Broker
Принадлежит: Behringwerke AG
Опубликовано: 09-06-1991
Автор(ы): Mathias Grote, Michael Broker
Принадлежит: Behringwerke AG
Реферат: Abstract of the Disclosure A process for the expression of foreign genes in yeasts The invention relates to the optimization of the expres-sion of foreign genes, especially recombinant factor XIIIa (rFXIIIa) in yeasts. The optimization was essen-tially carried out by use of a hybrid Gal UAS /CycI promoter, deletion of the 5' non-translated region and truncation of the 3' non-translated region of the FXIIIa cDNA, selection of specific yeast strains and a par-ticularly suitable growth medium composed of whey or whey hydroly ate. Quantities achievable in this way are ? 100 mg/l FXIIIa in shake cultures and ? 500 mg/l in fermentation after scale-up.
Promoter for expressing foreign genes in monocotyledonous plants
Номер патента: US5866793A. Автор: Monica Baga,Ravindra N. Chibbar,Kutty K. Kartha. Владелец: NATIONAL RESEARCH COUNCIL OF CANADA. Дата публикации: 1999-02-02.