Magnetizable moieties in separation, sequencing and amplification of polynucleotides and polypeptides and magnetic detection thereof
Номер патента: EP0781346A4
Опубликовано: 08-12-1999
Автор(ы): Lonnie W Adelman
Принадлежит: Lonnie W Adelman
Опубликовано: 08-12-1999
Автор(ы): Lonnie W Adelman
Принадлежит: Lonnie W Adelman
Реферат: A method for sequencing nucleotides by attaching magnetizable moieties to the components of a mixture of such polynucleotides, proteins and fragments, subjecting the mixture to a separation procedure to distribute the compounds on a substrate in a pattern according to molecular size and quantity, imparting magnetic properties to the attached magnetizable moieties, and then subjecting the substrate to magnetic reading to determine the molecular size and quantity of the compounds. Also disclosed is a method for monitoring a molecule amplification process wherein primers are used to produce copies of a molecule, which primers become part of the copy of the molecule by attaching a magnetized moiety to each primer such that the copy has magnetizable moieties at each end. The magnetizable moieties may then be magnetized. By subjecting the mixture to a magnetic field, the primers in the mixture can be separated from the copies produced and then the copies produced can be analyzed by a magnetic field sensor to determine the amount of copies produced.
In vivo labelling of polynucleotide sequences
Номер патента: US4987065A. Автор: Jannis Stavrianopoulos,Norman E. Kelker,Heuy-Lang Yang. Владелец: Enzo Biochem Inc. Дата публикации: 1991-01-22.