Baculovirus expression system capable of producing foreign gene proteins at high levels
Номер патента: CA1330425C
Опубликовано: 28-06-1994
Автор(ы): C. Yong Kang
Принадлежит: UNIVERSITY OF OTTAWA
Опубликовано: 28-06-1994
Автор(ы): C. Yong Kang
Принадлежит: UNIVERSITY OF OTTAWA
Реферат: Title: IMPROVED BACULOVIRUS EXPRESSION SYSTEM CAPABLE OF PRODUCING FOREIGN GENE PROTEINS AT HIGH LEVELS ABSTRACT OF THE DISCLOSURE A baculovirus expression system capable of producing foreign gene proteins at high levels. The system involves the production of a recombinant baculovirus containing a modified foreign gene between the polyhedrin gene promoter region and the transcription termination signal of the polyhedrin structural gene. The modified foreign gene comprises a putative ribosome binding site immediately upstream of the foreign gene coding sequence, i.e. without any intervening non-coding sequences. The putative ribosome binding site is preferably properly positioned without the intervening sequences by a crossover linker mutagenesis procedure before the modified foreign gene is introduced into the virus. The putative ribosome binding site preferably comprises at least the final four nucleotides of the sequence ACCTATAAAT immediately upstream of the translation initiation codon (ATG) of the foreign gene. The system is capable of producing foreign gene proteins (when insect cells are infected with the recombinant virus) at high levels, even in the case of those genes which expressed only at low or intermediate levels in prior recombinant baculovirus systems.
Hepatitis C virus second envelope (HCV-E2) glycoprotein expression system
Номер патента: US6020122A. Автор: Gregory F. Okasinski,Verlyn G. Schaefer,Thomas S. Suhar,Richard R. Lesniewski. Владелец: ABBOTT LABORATORIES. Дата публикации: 2000-02-01.