28-01-2016 дата публикации
Номер: US20160024155A1
Принадлежит:
Polypeptides are disclosed herein, which recognize and are strong binders to Influenza A hemagglutinin and can be used, for example, to treat and/or limit development of an influenza infection. 1. A polypeptide comprising an amino acid sequence according to general formula IIR1-R2-R3-R4-R5-R6-R7-R8-R9-Ala-R10-R11-Phe (SEQ ID NO: 83), whereinR1 is selected from the group consisting of Phe and Val;R2 is selected from the group consisting of Ser, Ala, Phe, Gly, Ile, Lys, Leu, Met, Asn, Pro, Gln, Arg, Thr, and Val;R3 is selected from the group consisting of Glu, and Asp;R4 is selected from the group consisting of Asn, His, Ile, Lys, Leu, Met, Arg, Ser, and Thr;R5 is selected from the group consisting of Leu, Phe, Ile, Met, Asn, Gln, and Val;R6 is selected from the group consisting of Ala, Asp, Lys, Met, Asn, Gln, Arg, Glu, and Val;R7 is selected from the group consisting of Phe, Asp, Asn, and Tyr;R8 is selected from the group consisting of Glu, Ala, Asp, Gly, His, Lys, Leu, Met, Asn, Gln, Arg, Ser, Thr, Val, and Tip;R9 is selected from the group consisting of Leu, Phe, Ile, Met, and Val;R10 is selected from the group consisting of Leu, Ile, Met, and Tyr; andR11 is selected from the group consisting of Ser, Ala, Gly, and Tyr;3. The polypeptide of claim 2 , wherein X1 comprises the amino acid sequence TNKDTPDRW-Z1-KVA (SEQ ID NO: 85) where Z1 is Ala claim 2 , Lys claim 2 , Arg claim 2 , Gly claim 2 , or Thr.4. The polypeptide of claim 2 , wherein general formula II is A1-R1-R2-R3-R4-R5-R6-R7-R8-R9-Ala-R10-R11-Phe-X1-R12-R13-X2-R14-B1 (SEQ ID NO: 86) claim 2 , wherein one or both of A1 and B1 are present claim 2 , and wherein:A1 comprises the amino acid sequence: Z2-ASTRGSGRPW-Z3 (SEQ ID NO: 87), wherein Z2 is absent or is Met, and Z3 is selected from group consisting of Gly, Arg, Lys, Asp andB1 comprises the amino acid sequence G-Z4-TPEEVKKHYE (SEQ ID NO: 88), where Z4 is R or K.10. The polypeptide of claim 1 , wherein the polypeptide comprises a detectable tag.11. An ...
Подробнее