Beta-caryophyllene synthetase as well as gene, strain and application thereof

The invention discloses beta-caryophyllene synthetase as well as a gene, a strain and application thereof. According to the invention, it is found for the first time that an enzyme derived from hevea brasiliensis and having an amino acid sequence as shown in SEQ ID NO.1 is a beta-caryophyllene synthetase, or a coding gene of the beta-caryophyllene synthetase or a recombinant plasmid containing the coding gene of the beta-caryophyllene synthetase, and a recombinant strain containing the coding gene of the beta-caryophyllene synthetase can be used for synthesizing or producing beta-caryophyllene. The beta-caryophyllene high-producing strain is obtained by introducing a coding gene of beta-caryophyllene synthetase into a microorganism which overexpresses one or more genes in a mevalonic acid pathway and a farnesene pyrophosphate synthase gene. The yield of the beta-caryophyllene high-producing strain provided by the invention in a fermentation tank (5L steel tank) reaches 6.2 g/L. The prepared ...