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Применить Всего найдено 2. Отображено 2.
15-08-2023 дата публикации

Primer, probe and method for detecting helicobacter pylori through ddPCR

Номер: CN116590440A
Автор: FAN HONGBO, HU LIANGYONG, CAI YONGHONG, TANG XIAOJUN, ZHANG LILING, MA TINGTING, YUAN XIAODONG
Принадлежит:

The invention discloses a primer, a probe and a method for detecting helicobacter pylori through ddPCR. The primer comprises primers for detecting 23SrRNA of the helicobacter pylori as shown in SEQ ID NO.1 and SEQ ID NO.2, and primers for detecting ureC genes of the helicobacter pylori as shown in SEQ ID NO.4 and SEQ ID NO.5. The invention further discloses a kit for detecting the helicobacter pylori through ddPCR. The method for detecting the helicobacter pylori 23SrRNA and the ureC gene through ddPCR is established, the demand quantity of samples is low, the detection sensitivity is high, and a trace amount of helicobacter pylori 23SrRNA and the ureC gene can be detected; absolute quantitative analysis of helicobacter pylori can be directly carried out without depending on standard curves of an analysis sample and a reference standard; the method is sensitive, simple, rapid and high in accuracy, and can be widely applied to the fields related to scientific research and medical detection ...

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Номер записи: 1
25-04-2023 дата публикации

Specific primer, probe and method for detecting helicobacter pylori based on ddPCR method

Номер: CN116004875A
Автор: FAN HONGBO, HU LIANGYONG, HU SONGQING, CAI YONGHONG, LI YUEYUE, LIANG ZHIJIAN, HUANG MANYING
Принадлежит:

The invention discloses a specific primer, a probe and a method for detecting helicobacter pylori based on a ddPCR method. The specific primer comprises a forward primer with a sequence as shown in SEQ ID NO.1 and a reverse primer with a sequence as shown in SEQ ID NO.2. The invention further discloses a kit for detecting helicobacter pylori based on the ddPCR method. In the invention, the primer and the probe are designed according to the helicobacter pylori 16SrDNA, and the reaction system and the reaction procedure of the ddPCR amplification reaction are optimized, so that the method for detecting the helicobacter pylori based on the ddPCR method is finally established, the sensitivity is high, the repeatability is strong, a more accurate and efficient detection scheme is provided for helicobacter pylori detection, and the method is suitable for popularization and application. The method can be widely applied to scientific research and medical detection related fields.

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Номер записи: 2