Labeling reagent and methods of use

The present invention provides compounds which are useful as multifunctional labels in proteomics studies. The labels of the present invention are both lysine specific and increase the overall sequence coverage obtained in polypeptide mapping experiments, by for example, increasing the ionization efficiencies of lysine-terminated tryptic fragments. In certain aspects, the labels of the present invention can be used to measure differential quantitation, as for example, deuterium(s) can easily be introduced during their synthesis. In one aspect, a C-terminal derivatized lysine biases the fragment ion intensities strongly toward C-terminal fragment ions, resulting in a highly simplified tandem mass spectrum. In further aspects, the number of lysine residues can be determined in a polypeptide.

LABELING REAGENT AND METHODS OF USE

The present invention provides compounds which are useful as multifunctional labels in proteomics studies. The labels of the present invention are both lysine specific and increase the overall sequence coverage obtained in polypeptide mapping experiments, by for example, increasing the ionization efficiencies of lysine-terminated tryptic fragments. In certain aspects, the labels of the present invention can be used to measure differential quantitation, as for example, deuterium(s) can easily be introduced during their synthesis. In one aspect, a C-terminal derivatized lysine biases the fragment ion intensities strongly toward C-terminal fragment ions, resulting in a highly simplified tandem mass spectrum. In further aspects, the number of lysine residues can be determined in a polypeptide.

Fluid drive means for a linear reciprocating motion

A fluid drive means for a linear reciprocating motion is provided with at least one flexible and collapsible member (15) arranged between elements (11, 12, 14) which move in relation to each other during operation. The member (15) surrounds between said elements (11, 12, 14) a central rod (13), which extends through one of the elements (11, 12). Together with the rod the member (15) completely delimits a fluid chamber for the pressure fluid which is required for the operation of the drive means. The member (15), which is made for instance of an elastomer, consists of a roll-membrane and establishes a sealing (19) around the rod (13).

Apparatus for cleaning filters for liquids

Fluid drive means for a reciprocating motion

Abstract Of The Disclosure A fluid drive means for a linear reciprocating motion is provided with at least one flexible and collapsible member (15) arranged between elements (11, 12, 14) which move in relation to each other during operation. The member (15) surrounds between said elements (11, 12, 14) a central rod (13), which extends through one of the elements (11, 12). Together with the rod the member (15) completely delimits a fluid chamber for the pressure fluid which is required for the operation of the drive means. The member (15) which is made for instance of an elastomer constist of a roll-membrane and establishes a sealing (19) around the rod (13).

Markierungsreagens und anwendungsverfahren

Thermal expansion pump for pulse-free liquid flow

Pulse-free flow, for instance for capillary chromatography, is provided by a pump comprised of a length of heat transmissive tubing filled with a liquid that undergoes thermal expansion upon heating, in conjunction with a heating device that provides uniform heating of the tubing and its contents at a controlled rate.

Labeling reagent and methods of use

The present invention provides compounds which are useful as multifunctional labels in proteomics studies. The labels of the present invention are both lysine specific and increase the overall sequence coverage obtained in polypeptide mapping experiments, by for example, increasing the ionization efficiencies of lysine-terminated tryptic fragments. In certain aspects, the labels of the present invention can be used to measure differential quantitation, as for example, deuterium(s) can easily be introduced during their synthesis. In one aspect, a C-terminal derivatized lysine biases the fragment ion intensities strongly toward C-terminal fragment ions, resulting in a highly simplified tandem mass spectrum. In further aspects, the number of lysine residues can be determined in a polypeptide.

Labeling reagent and methods of use

The present invention provides compounds which are useful as multifunctional labels in proteomics studies. The labels of the present invention are both lysine specific and increase the overall sequence coverage obtained in polypeptide mapping experiments, by for example, increasing the ionization efficiencies of lysine-terminated tryptic fragments. In certain aspects, the labels of the present invention can be used to measure differential quantitation, as for example, deuterium(s) can easily be introduced during their synthesis. In one aspect, a C-terminal derivatized lysine biases the fragment ion intensities strongly toward C-terminal fragment ions, resulting in a highly simplified tandem mass spectrum. In further aspects, the number of lysine residues can be determined in a polypeptide.

Fluidumdrevet dreisanordning.

Fluidumdrevet dreieanordning, omfattende en utgående dreieaksel (6) som er lagret i et hus (1,2). og en i huset forskyvbar plate (3). Mellom platen og dreieakselen finnes en mekanisme (4,5) til overføring av bevegelse. På i det minste den ene side av platen finnes et trykkammer som er tilsluttet en trykkfluidumkilde. Mekanismen til bevegelsesoverføring dannes av en med platen (3) fast forbundet tapp (4) og en aksialt snodd sliss (5) som strekker seg gjennom dreieakselen (6) og opptar tappen (4).

Fluiddrivet vriddon med en rorelseoverforingsmekanism mellan en platta och en utgaende vridaxel

Fluiddrivet vriddon

Lock till fluidbehallare