HEXURONATE C4-EPIMERASE VARIANT HAVING IMPROVED D-TAGATOSE CONVERSION ACTIVITY, AND D-TAGATOSE PRODUCTION METHOD USING SAME

Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them. 1. A hexuronate C4-epimerase variant in which one or more amino acid residue selected from the group consisting of leucine (L) as an amino acid residue at position 77 , serine (S) residue at position 125 , alanine (A) residue at position 158 , and proline (P) residue at position 351 , from the N-terminus of a hexuronate C4-epimerase consisting of the amino acid sequence set forth in SEQ ID NO: 1 are mutated.2. The hexuronate C4-epimerase variant according to claim 1 , wherein the leucine (L) residue at position 77 is replaced by proline (P) or arginine (R).3. The hexuronate C4-epimerase variant according to claim 1 , wherein the alanine (A) residue at position 158 is replaced by threonine (T).4. The hexuronate C4-epimerase variant according to claim 1 , wherein the serine (S) residue at position 125 is replaced by aspartic acid (D) claim 1 , glutamine (Q) claim 1 , glutamic acid (E) claim 1 , threonine (T) claim 1 , asparagine (N) claim 1 , cysteine (C) claim 1 , or tyrosine (Y).5. The hexuronate C4-epimerase variant according to claim 1 , wherein the proline (P) residue at position 351 is replaced by serine (S).6. The hexuronate C4-epimerase variant according to claim 1 , wherein one or more amino acid residue selected from the group consisting of glutamine (Q) residue at position 149 claim 1 , lysine (K) residue at position 164 claim 1 , aspartic acid (D) residue at position 168 claim 1 , glutamic acid (E) residue at position 175 claim 1 , and valine (V) residue at position 267 claim 1 , from the N-terminus of the hexuronate C4-epimerase are further mutated.7. The hexuronate C4-epimerase variant according to claim 1 , wherein in case the leucine (L) residue at position 77 is replaced by proline (P) or arginine (R) claim 1 , one or more amino acid residue selected from the group ...

NOVEL PSICOSE-6-PHOSPHATE PHOSPHATASE, COMPOSITION FOR PRODUCING PSICOSE COMPRISING THE SAME, AND METHOD FOR PRODUCING PSICOSE USING THE SAME

The present application relates to a psicose-6-phosphate phosphatase, a microorganism comprising the same, and a method for producing psicose using the same. 118-. (canceled)19Alicyclobacillus, Amycolatopsis, Anaerolinea, Archaeoglobus, Bacillus, Caldicellulosiruptor, Caldilinea, Caldithrix, Carboxydocella, Carboxydothermus, Chloroflexi, Defluviitoga, Deinococcus, Desulfurococcus, Dictyoglomus, Effusibacillus, Fervidobacterium, Geobacillus, Halococcus, Hydrogenivirga, Hydrogenobacter, Hyperthermus, Kosmotoga, Marinitoga, Meiothermus, Mesotoga, Metallosphaera, Methanocella, Methanococcoides, Methanohalobium, Methanolobus, Methanosarcina, Methanothermus, Petrotoga, Picrophilus, Pseudonocardia, Pyrococcus, Pyrodictium, Rhodothermus, Slackia, Staphylothermus, Sulfolobus, Thermanaerothrix, Thermoanaerobacter, Thermoanaerobacterium, Thermobifida, Thermococcus, Thermocrinis, Thermoflexus, Thermotoga, ThermusTruepera.. A method for producing psicose , comprising converting psicose-6-phosphate into psicose by bringing psicose-6-phosphate into contact with a psicose-6-phosphate phosphatase , a microorganism expressing the same , or a culture of the microorganism , wherein the psicose-6-phosphate phosphatase is derived from any one selected from the group consisting of the genus , and20. The method of claim 19 , wherein the method further comprises converting fructose-6-phosphate into psicose-6-phosphate by bringing fructose-6-phosphate into contact with fructose-6-phosphate-3-epimerase claim 19 , a microorganism expressing the same claim 19 , or a culture of the microorganism claim 19 , prior to the converting psicose-6-phosphate into psicose.21. The method of claim 20 , wherein the method further comprises converting glucose-6-phosphate into fructose-6-phosphate by bringing glucose-6-phosphate into contact with glucose-6-phosphate-isomerase claim 20 , a microorganism expressing the same claim 20 , or a culture of the microorganism claim 20 , prior to the converting fructose- ...

COMPOSITION FOR PRODUCING TAGATOSE AND METHOD OF PRODUCING TAGATOSE USING THE SAME

Provided are a composition for producing tagatose, comprising fructose-4-epimerase, and a method of producing tagatose using the same. 1. A composition for producing tagatose , comprising tagatose-6-phosphate kinase , a microorganism expressing the tagatose-6-phosphate kinase , or a culture of the microorganism.2. The composition for producing tagatose of claim 1 , further comprising fructose.3. The composition for producing tagatose of claim 1 , wherein the composition comprises one or more of tagatose-6-phosphate kinase consisting of an amino acid sequence of SEQ ID NO: 1 claim 1 , 3 claim 1 , 5 claim 1 , 7 claim 1 , 9 claim 1 , or 11.4AnaerolineaeThermobifidaThermoanaerobacterDictyoglomus. The composition for producing tagatose of claim 1 , wherein the tagatose-6-phosphate kinase is an enzyme derived from sp. claim 1 , the genus of claim 1 , the genus of claim 1 , or the genus of claim 1 , or a variant thereof.5. A method of producing tagatose claim 1 , comprising converting fructose into tagatose by contacting fructose with tagatose-6-phosphate kinase claim 1 , a microorganism expressing the tagatose-6-phosphate kinase claim 1 , or a culture of the microorganism.6. The method of producing tagatose of claim 5 , wherein the contacting is performed under conditions of pH 5.0 to pH 9.0 and 30° C. to 80° C. for 0.5 hours to 48 hours. The present application is a continuation-in-part of International Application No. PCT/KR2018/003769, filed Mar. 30, 2018, which claims the benefit of Korean application nos. 10-2017-0158766, filed Nov. 24, 2017, 10-2017-0111494, filed Aug. 31, 2017, and 10-2017-0042166, filed Mar. 31, 2017, the contents of which are hereby incorporated by reference in their entireties.The present disclosure relates to a composition for producing tagatose, comprising fructose-4-epimerase, and a method of producing tagatose using the same.Tagatose is a natural sweetener, which is present in a small amount in foods such as milk, cheese, cacao, etc., and in ...

HEXURONATE C4-EPIMERASE VARIANT HAVING IMPROVED D-TAGATOSE CONVERSION ACTIVITY, AND D-TAGATOSE PRODUCTION METHOD USING SAME

Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them. 1. A hexuronate C4-epimerase variant in which threonine (T) as an amino acid residue at position 272 from the N-terminus of a hexuronate C4-epimerase consisting of the amino acid sequence set forth in SEQ ID NO: 1 is mutated.2. The hexuronate C4-epimerase variant according to claim 1 , wherein the threonine (T) residue at position 272 is replaced by alanine (A) claim 1 , aspartic acid (D) claim 1 , glutamic acid (E) claim 1 , phenylalanine (F) claim 1 , glycine (G) claim 1 , histidine (H) claim 1 , isoleucine (I) claim 1 , lysine (K) claim 1 , leucine (L) claim 1 , methionine (M) claim 1 , asparagine (N) claim 1 , proline (P) claim 1 , glutamine (Q) claim 1 , arginine (R) claim 1 , serine (S) claim 1 , valine (V) or tyrosine (Y).3. The hexuronate C4-epimerase variant according to claim 1 , wherein the serine (S) residue at position 125 from the N-terminus of the hexuronate C4-epimerase is further mutated.4. The hexuronate C4-epimerase variant according to claim 3 , wherein the serine (S) residue at position 125 is replaced by cysteine (C) claim 3 , tyrosine (Y) claim 3 , glutamine (Q) claim 3 , glutamic acid (E) claim 3 , threonine (T) claim 3 , asparagine (N) or aspartic acid (D).5. The hexuronate C4-epimerase variant according to claim 3 , wherein (i) the serine (S) residue at position 185 or (ii) the valine (V) residue at position 267 claim 3 , the serine (S) residue at position 268 or a combination of the valine (V) residue at position 267 and the serine (S) residue at position 268 from the N-terminus of the hexuronate C4-epimerase is further mutated.6. The hexuronate C4-epimerase variant according to claim 5 , wherein the serine (S) residue at position 185 is replaced by alanine (A) claim 5 , glycine (G) claim 5 , histidine (H) claim 5 , lysine (K) claim 5 , glutamine (Q) or ...

PRODUCTION METHOD FOR TAGATOSE

A method for producing tagatose comprises: a) performing epimerization of fructose using hexuronate C4-epimerase to obtain an epimerized product comprising tagatose; b) purifying the epimerized product; and c) crystallizing the purified epimerized product. The hexuronate C4-epimerase is an enzyme derived from or mutants thereof. The hexuronate C4-epimerase is produced from strains , or 1. A method for producing tagatose , comprising:a) performing epimerization of fructose using hexuronate C4-epimerase to obtain an epimerized product comprising tagatose; andb) purifying the epimerized product.2Thermotoga maritima, Thermotoga neapolitana, Thermotoga thermarum. The method for producing tagatose according to claim 1 , wherein the hexuronate C4-epimerase is an enzyme derived from or mutants thereof.3Escherichia. coli, Corynebacterum glutamicum, Aspergillus oryzaeBacillus subtilis.. The method for producing tagatose according to claim 1 , wherein the hexuronate C4-epimerase is produced from strains claim 1 , or4E. coliE. coliE. coli. The method for producing tagatose according to claim 3 , wherein the hexuronate C4-epimerase production strain is BL21(DE3) pET21a-TM (accession number: KCCM11542P) claim 3 , BL21(DE3) pET21a-TN (accession number: KCCM11543P) claim 3 , or BL21(DE3) pET28a-TN(m) (accession number: KCCM11544P).5. The method for producing tagatose according to claim 1 , wherein the epimerization step comprises reacting fructose and hexuronate C4-epimerase at a temperature ranging from 60° C. to 90° C. and pH 5 to 8.6. The method for producing tagatose according to claim 5 , wherein the epimerization step is performed in the presence of a metal salt.7. The method for producing tagatose according to claim 6 , wherein the metal salt comprises at least one of NiSO claim 6 , NiCl claim 6 , CoCl claim 6 , MnCl claim 6 , and ZnSO.8. The method for producing tagatose according to claim 1 , wherein the epimerized product comprises tagatose in an amount of 0.05 wt % or ...

Hexuronate c4-epimerase mutant with improved conversion activity, and method for producing d-tagatose by using same

A hexuronate C4-epimerase with improved conversion activity and a method for producing D-tagatose using the hexuronate C4-epimerase. The hexuronate C4-epimerase includes an amino acid sequence set forth in SEQ ID NO: 1, in which serine (S) at position 125, serine (S) at position 185, valine (V) at position 267, serine (S) at position 268, threonine (T) at position 272, tryptophan (W) at position 306, arginine (R) at position 386 and tyrosine (Y) at position 403 from an N-terminal of hexunorate C4-epimerase are mutated.

HEXURONATE C4-EPIMERASE MUTANT WITH IMPROVED CONVERSION ACTIVITY, AND METHOD FOR PRODUCING D-TAGATOSE BY USING SAME

A hexuronate C4-epimerase with improved conversion activity and a method for producing D-tagatose using the hexuronate C4-epimerase. The hexuronate C4-epimerase includes an amino acid sequence set forth in SEQ ID NO: 1, in which serine (S) at position 125, serine (S) at position 185, valine (V) at position 267, serine (S) at position 268, threonine (T) at position 272, tryptophan (W) at position 306, arginine (R) at position 386 and tyrosine (Y) at position 403 from an N-terminal of hexunorate C4-epimerase are mutated. 1. A hexuronate C4-epimerase variant having an amino acid sequence set forth in SEQ ID NO: 1 , in which tyrosine (Y) at position 403 , serine (S) at position 125 , serine (S) at position 185 , valine (V) at position 267 , serine (S) at position 268 , threonine (T) at position 272 , tryptophan (W) at position 306 and arginine (R) at position 386 from an N-terminal of hexunorate C4-epimerase are mutated.2. The hexuronate C4-epimerase variant according to claim 1 , whereintyrosine (Y) at position 403 is substituted with alanine (A), cysteine (C), aspartic acid (D), glutamic acid (E), phenylalanine (F), glycine (G), histidine (H), isoleucine (I), lysine (K), leucine (L), methionine (M), asparagine (N), proline (P), glutamine (Q), arginine (R), serine (S), threonine (T), valine (V), or tryptophan (W),serine (S) at position 125 is substituted with cysteine (C), tyrosine (Y), glutamine (Q), glutamic acid (E), threonine (T), asparagine (N), or aspartic acid (D),serine (S) at position 185 is substituted with alanine (A), glycine (G), histidine (H), lysine (K), glutamine (Q), or arginine (R),valine (V) at position 267 is substituted with methionine (M),serine (S) at position 268 is substituted with cysteine (C), or threonine (T),threonine (T) at position 272 is substituted with alanine (A), aspartic acid (D), glutamic acid (E), phenylalanine (F), glycine (G), histidine (H), isoleucine (I), lysine (K), leucine (L), methionine (M), glutamine (Q), arginine (R), ...

Composition for preparing tagatose and method for preparing tagatose from fructose

The present invention relates to a composition for preparing tagatose, wherein the composition is used for preparing tagatose from fructose and contains a protein including any one amino acid sequence of SEQ ID NOS: 1 to 7 or a microorganism expressing the protein. In addition, the present invention relates to a method for preparing tagatose from fructose, the method comprising a step of allowing the composition to react with fructose.

HEXURONATE C4-EPIMERASE VARIANT HAVING IMPROVED D-TAGATOSE CONVERSION ACTIVITY, AND D-TAGATOSE PRODUCTION METHOD USING SAME

Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them. 1. A hexuronate C4-epimerase variant in which a tyrosine (Y)-403 amino acid residue from N-terminal of a hexuronate C4-epimerase consisting of an amino acid sequence of SEQ ID NO: 1 is mutated.2. The hexuronate C4-epimerase variant of claim 1 , wherein the tyrosine (Y)-403 amino acid residue is substituted with alanine (A) claim 1 , cysteine (C) claim 1 , aspartic acid (D) claim 1 , glutamic acid (E) claim 1 , phenylalanine (F) claim 1 , glycine (G) claim 1 , histidine (H) claim 1 , isoleucine (I) claim 1 , lysine (K) claim 1 , leucine (L) claim 1 , methionine (M) claim 1 , asparagine (N) claim 1 , proline (P) claim 1 , glutamine (Q) claim 1 , arginine (R) claim 1 , serine (S) claim 1 , threonine (T) claim 1 , valine (V) or tryptophan (W).3. The hexuronate C4-epimerase variant of claim 1 , wherein in the hexuronate C4-epimerase variant claim 1 , a serine (S)-125 amino acid residue from the N-terminal of the hexuronate C4-epimerase is additionally mutated.4. The hexuronate C4-epimerase variant of claim 3 , wherein the serine (S)-125 amino acid residue is substituted with cysteine (C) claim 3 , tyrosine (Y) claim 3 , glutamine (Q) claim 3 , glutamic acid (E) claim 3 , threonine (T) claim 3 , asparagine (N) claim 3 , or aspartic acid (D).5. The hexuronate C4-epimerase variant of claim 3 , wherein in the hexuronate C4-epimerase variant claim 3 , (i) one to five amino acid residues selected from the group consisting of a serine (S)-185 amino acid residue claim 3 , a valine (V)-267 amino acid residue claim 3 , a serine (S)-268 amino acid residue claim 3 , a threonine (T)-272 amino acid residue claim 3 , a tryptophan (W)-306 amino acid residue claim 3 , and an arginine (R)-386 amino acid residue claim 3 , or (ii) a leucine (L)-77 amino acid residue claim 3 , an alanine (A)-158 amino ...

HEXURONATE C4-EPIMERASE VARIANT HAVING IMPROVED D-TAGATOSE CONVERSION ACTIVITY, AND D-TAGATOSE PRODUCTION METHOD USING SAME

Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them. 1. A hexuronate C4-epimerase variant in which serine (S) as an amino acid residue at position 185 from the N-terminus of a hexuronate C4-epimerase consisting of the amino acid sequence set forth in SEQ ID NO: 1 is mutated.2. The hexuronate C4-epimerase variant according to claim 1 , wherein the serine (S) residue at position 185 is replaced by alanine (A) claim 1 , glycine (G) claim 1 , histidine (H) claim 1 , lysine (K) claim 1 , glutamine (Q) or arginine (R).3. The hexuronate C4-epimerase variant according to claim 1 , wherein the serine (S) residue at position 125 from the N-terminus of the hexuronate C4-epimerase is further mutated.4. The hexuronate C4-epimerase variant according to claim 3 , wherein the serine (S) residue at position 125 is replaced by cysteine (C) claim 3 , tyrosine (Y) claim 3 , glutamine (Q) claim 3 , glutamic acid (E) claim 3 , threonine (T) claim 3 , asparagine (N) or aspartic acid (D).5. The hexuronate C4-epimerase variant according to claim 3 , wherein the serine (S) residue at position 268 from the N-terminus of the hexuronate C4-epimerase is further mutated.6. The hexuronate C4-epimerase variant according to claim 5 , wherein the serine (S) residue at position 268 is replaced by cysteine (C) or threonine (T).7. A nucleic acid encoding the hexuronate C4-epimerase variant according to .8. A transformant comprising the nucleic acid according to .9. A composition for D-tagatose production comprising the hexuronate C4-epimerase variant according to .10. A method for D-tagatose production comprising bringing the hexuronate C4-epimerase variant according to claim 1 , a microorganism or a culture thereof expressing the variant into contact with D-fructose.11. A method for D-tagatose production comprising bringing the hexuronate C4-epimerase variant ...

Dephosphorylation enzyme of new psicose-6-phosphoric acid, composition for producing psicose comprising same, and method for preparing psicose using same.

La presente solicitud se refiere a una psicosa-6-fosfato fosfatasa, un microorganismo que comprende la misma y un procedimiento para la producción de psicosa mediante el uso de la misma.

Hexuronate c4-epimerase variant having improved d-tagatose conversion activity, and d-tagatose production method using same

Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.

FRUCTOSE-4-EPIMERASE AND TAGATOSE PRODUCTION METHOD THROUGH ITS USE

Se proporciona una variante de fructosa-4-epimerasa que tiene actividad de conversión de tagatosa, y un método de preparación de tagatosa mediante su uso. A fructose-4-epimerase variant having tagatose converting activity, and a method of preparing tagatose by its use, are provided.

Composition for producing tagatose and method of producing tagatose using the same

The present application relates to a composition for tagatose production containing fructose-4-epimerase, and a method for tagatose production using the same.

composition for the production of tagatose, and, method of production of tagatose.

trata-se de uma composição para produção de tagatose, que compreende frutose-4-epimerase, e um método de produção de tagatose que usa a mesma. it is a composition for the production of tagatose, which comprises fructose-4-epimerase, and a method of producing tagatose that uses it.

Composition for preparing tagatose and method for preparing tagatose from fructose

Hexuronate c4-epimerase variant having improved d-tagatose conversion activity, and d-tagatose production method using same

Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.

Composition for preparing tagatose and method for preparing tagatose from fructose

The present invention relates to a composition for preparing tagatose, wherein the composition is used for preparing tagatose from fructose and contains a protein including any one amino acid sequence of SEQ ID NOS: 1 to 7 or a microorganism expressing the protein. In addition, the present invention relates to a method for preparing tagatose from fructose, the method comprising a step of allowing the composition to react with fructose.

Production method for tagatose

The present invention relates to a production method for tagatose.

Hexuronate C4-epimerase variant having improved D-tagatose conversion activity, and D-tagatose production method using same

Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.

Hexuronate C4-epimerase mutant with improved conversion activity, and method for producing D-tagatose by using same

A hexuronate C4-epimerase with improved conversion activity and a method for producing D-tagatose using the hexuronate C4-epimerase. The hexuronate C4-epimerase includes an amino acid sequence set forth in SEQ ID NO: 1, in which serine (S) at position 125, serine (S) at position 185, valine (V) at position 267, serine (S) at position 268, threonine (T) at position 272, tryptophan (W) at position 306, arginine (R) at position 386 and tyrosine (Y) at position 403 from an N-terminal of hexunorate C4-epimerase are mutated.

Novel fructose-4-epimerase and method for preparing tagatose using same

副反応性が低いリブロースリン酸３－エピメラーゼのモチーフ及びそれを含む酵素

【課題】リブロースリン酸３－エピメラーゼ、それを含む微生物及び組成物、それを用いたプシコース－６－リン酸又はプシコース製造方法を提供する。【解決手段】特定のアミノ酸配列からなるモチーフＩ、及び特定のアミノ酸配列からなるモチーフＩＩＩを含むリブロースリン酸３－エピメラーゼを提供し、前記リブロースリン酸３－エピメラーゼは、プシコースをフルクトースに変換する活性がないか、又は５％以下である。【選択図】なし

Hexuronate c4-epimerase variant with improved d-tagatose production activity, and d-tagatose production method using same

Hexuronate c4-epimerase variant with improved d-tagatose production activity, and d-tagatose production method using same

Ribulose-phosphate 3-epimerase motif having lower side reactivity and enzyme comprising same

Provided are ribulose-phosphate 3-epimerase, a microorganism and a composition, each including the ribulose-phosphate 3-epimerase, and a method of producing psicose-6-phosphate or psicose using the same.

Ribulose-Phosphate 3-Epimerase Motif Having Low Side Reactivity And Enzyme Including The Same

The present application relates to ribulose-phosphate 3-epimerase, a microorganism and composition each comprising same, and a method for producing psicose-6-phosphate or psicose by using same.

Ribulose-Phosphate 3-Epimerase Motif Having Low Side Reactivity And Enzyme Including The Same

The present application relates to ribulose-phosphate 3-epimerase, a microorganism and composition each comprising same, and a method for producing psicose-6-phosphate or psicose by using same.

Ribulose-phosphate 3-epimerase motif having lower side reactivity and enzyme comprising same

Novel fructose-4-epimerase and tagatose production method using same

Provided are a fructose-4-epimerase variant having tagatose conversion activity, and a method of preparing tagatose using the same.

Novel fructose-4-epimerase and tagatose production method using same

Provided are a tagatose-bisphosphate aldolase variant having tagatose conversion activity, and a method of preparing tagatose using the same.

Novel fructose-4-epimerase and tagatose production method using same

Provided are a fructose-4-epimerase variant having tagatose conversion activity, and a method of producing tagatose using the same.

Fructose-4-epimerase and method of producing tagatose using the same

Provided are a tagatose-bisphosphate aldolase variant having tagatose conversion activity, and a method of preparing tagatose using the same.

Fructose-4-epimerase and method of producing tagatose using the same

Provided are a fructose-4-epimerase variant having tagatose conversion activity, and a method of preparing tagatose using the same.

Fructose-4-epimerase and method for preparing tagatose using same

The present disclosure relates to a fructose-C4-epimerase and a method of preparing tagatose using the same.