17-05-2024 дата публикации
Номер: NL2033410B1
The invention provides a method for providing a cargo-conjugated protein (130), wherein the method comprises: a first conjugation stage (510) comprising exposing a protein (100) to a linker (20) to provide a linker-conjugated protein (120), wherein the linker (20) has a structure according to formula (I): ? N / \ R wherein R comprises a first click chemistry group (21) selected from the group comprising a 10 2-azatricyclo[10.4.0.04’9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl moiety, a bicyclo[6.l.0]non-4-yne moiety, an (E)—1-(cyclooct-4-en-1-yloxy) moiety, an azide moiety, a terminal alkyne moiety, a 4-(6-methyl-1,2,4,5-tetrazin-3 -yl)phenyl moiety, a 2- (cyanobenzo[d]thiazol-6-yl)amino moiety, and a 1,2-aminothiol moiety, a second conjugation stage (520) comprising eXposing the linker-conjugated protein (120) to a cargo (30) to provide 15 the cargo-conjugated protein (130), wherein the cargo (30) comprises a second click chemistry group (31), wherein the second click chemistry group (31) is configured to conjugate to the first click chemistry group (21).
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