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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Применить Всего найдено 4. Отображено 4.
07-03-2024 дата публикации

Single-molecule aptamer fret for protein identification and structural analysis

Номер: WO2024049290A1
Автор: Chirlmin JOO, Mike FILIUS, Raman Gautam VAN WEE
Принадлежит: Technische Universiteit Delft

The invention provides a method for characterization of a structure of a protein using a first probe and a second probe, wherein the method comprises: an exposure stage comprising: (i) exposing the protein to the second probe, (ii) providing radiation to the protein, wherein the radiation has a wavelength selected from a donor excitation radiation range, and (iii) measuring emission in a donor emission radiation range and an acceptor emission radiation range to provide an emission signal; wherein the exposure stage is protein degradation-free; and wherein: the protein comprises a first binding site and a second binding site; the first probe is: (i) covalently bound to the protein at the first binding site; or (ii) configured to transiently bind the protein at the first binding site, wherein the first probe comprises a first chromophore; the second probe is configured to transiently bind the protein at the second binding site with an off-rate selected from the range of 0.01 – 10 s-1, wherein the second probe comprises a second chromophore, wherein the second probe comprises an affinity-based probe selected from the group comprising an aptamer, an antibody, a nanobody, and a small-molecule moiety; the first chromophore and the second chromophore are selected from FRET donor-acceptor pair chromophores, wherein the FRET donor-acceptor pair chromophores have the donor excitation radiation range, the donor emission radiation range and the acceptor emission radiation range, wherein a donor of the FRET donor-acceptor pair chromophores is excitable by donor excitation radiation in the donor excitation radiation range, wherein an acceptor of the FRET donor-acceptor pair chromophores is configured to provide acceptor emission radiation in the acceptor emission radiation range upon excitation with donor excitation radiation of the donor when the first chromophore and the second chromophore are configured within a FRET distance selected from the range of 0.1 – 10 nm.

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Номер записи: 1
15-03-2024 дата публикации

Single-molecule aptamer FRET for protein identification and structural analysis

Номер: NL2032916B1
Автор: Filius Mike, Gautam Van Wee Raman, Joo Chirlmin
Принадлежит: Univ Delft Tech

The invention provides a method for characterization of a structure (13) of a protein (10) using a first probe (31) and a second probe (32), wherein the method comprises: an exposure stage comprising: (i) exposing the protein (10) to the second probe (32), (ii) providing radiation (50) to the protein (10), wherein the radiation has a wavelength selected from a donor excitation radiation range, and (iii) measuring emission (60) in a donor emission 10 radiation range and an acceptor emission radiation range to provide an emission signal, wherein the exposure stage is protein degradation-free, and wherein: the protein (10) comprises a first binding site (11) and a second binding site (12), the first probe (31) is covalently bound to the protein (10) at a first binding site (11) or (ii) the first probe (31) is configured to transiently bind the protein (10) at the first binding site (11), Wherein the first probe (31) comprises a first 15 chromophore (21), the second probe (32) is configured to transiently bind the protein (10) at a second binding site (12) and comprises a second chromophore (22), wherein the second probe (32) comprises an affinity-based probe (35) selected from the group comprising an aptamer (36), an antibody, a nanobody, and a small-molecule moiety (37), the first chromophore (21) and the second chromophore (22) are selected from FRET donor-acceptor pair chromophores 20 (20), wherein the FRET donor-acceptor pair chromophores (20) have a donor excitation radiation range, the donor emission radiation range and the acceptor emission radiation range, wherein a donor (23) of the FRET donor-acceptor pair chromophores (20) is excitable by donor excitation radiation (53) in the donor excitation radiation range, Wherein an acceptor (24) of the FRET donor-acceptor pair chromophores (20) is configured to provide acceptor emission 25 radiation (64) in the acceptor emission radiation range upon excitation with donor excitation radiation (53) of the donor (23) when the first ...

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Номер записи: 2
02-05-2024 дата публикации

N-terminal protein modification

Номер: WO2024091120A1
Автор: Chirlmin JOO, Mike FILIUS, Zeshi LI
Принадлежит: Technische Universiteit Delft

The invention provides a method for providing a cargo-conjugated protein (130), wherein the method comprises: a first conjugation stage (510) comprising exposing a protein (100) to a linker (20) to provide a linker-conjugated protein (120), wherein the linker (20) has a structure according to formula (I): wherein R comprises a first click chemistry group (21) selected from the group comprising a 2-azatricyclo[10.4.0.0 4,9 ]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl moiety, a bicyclo[6.1.0]non-4-yne moiety, an (E)-1-(cyclooct-4-en-1-yloxy) moiety, an azide moiety, a terminal alkyne moiety, a 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl moiety, a 2- (cyanobenzo[d]thiazol-6-yl)amino moiety, and a 1,2-aminothiol moiety; a second conjugation stage (520) comprising exposing the linker-conjugated protein (120) to a cargo (30) to provide the cargo-conjugated protein (130), wherein the cargo (30) comprises a second click chemistry group (31), wherein the second click chemistry group (31) is configured to conjugate to the first click chemistry group (21).

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Номер записи: 3
17-05-2024 дата публикации

N-terminal protein modification

Номер: NL2033410B1
Автор: Filius Mike, Joo Chirlmin, Li Zeshi
Принадлежит: Univ Delft Tech

The invention provides a method for providing a cargo-conjugated protein (130), wherein the method comprises: a first conjugation stage (510) comprising exposing a protein (100) to a linker (20) to provide a linker-conjugated protein (120), wherein the linker (20) has a structure according to formula (I): ? N / \ R wherein R comprises a first click chemistry group (21) selected from the group comprising a 10 2-azatricyclo[10.4.0.04’9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl moiety, a bicyclo[6.l.0]non-4-yne moiety, an (E)—1-(cyclooct-4-en-1-yloxy) moiety, an azide moiety, a terminal alkyne moiety, a 4-(6-methyl-1,2,4,5-tetrazin-3 -yl)phenyl moiety, a 2- (cyanobenzo[d]thiazol-6-yl)amino moiety, and a 1,2-aminothiol moiety, a second conjugation stage (520) comprising eXposing the linker-conjugated protein (120) to a cargo (30) to provide 15 the cargo-conjugated protein (130), wherein the cargo (30) comprises a second click chemistry group (31), wherein the second click chemistry group (31) is configured to conjugate to the first click chemistry group (21).

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Номер записи: 4