08-10-2020 дата публикации
Номер: US20200320690A1
A method and system for preprocessing microscopic images. A method includes identifying a plurality of microglial cells shown in microglial cell channel images, wherein identifying the plurality of microglial cells further comprises quantizing the microglial cell channel microscopic images and filtering a plurality of objects in the quantized microglial cell channel microscopic images; identifying a soma and at least one projection for each of the plurality of microglial cells, wherein identifying the soma and the at least one projection for each microglial cell further includes iteratively removing pixels on the microglial cell as shown in one of the microglial cell channel images, wherein each soma and each projection has a respective size; and determining an activation state of each microglial cell based on the size of the soma and a total size of the at least one projection identified for the microglial cell. 1. A method for processing microscopic images , comprising:identifying a plurality of microglial cells shown in a plurality of microglial cell channel images, wherein identifying the plurality of microglial cells further comprises quantizing the plurality of microglial cell channel microscopic images and filtering a plurality of objects in the quantized plurality of microglial cell channel microscopic images;identifying a soma and at least one projection for each of the plurality of microglial cells, wherein identifying the soma and the at least one projection for each microglial cell further comprises iteratively removing pixels on the microglial cell as shown in one of the plurality of microglial cell channel images, wherein each soma and each projection has a respective size; anddetermining an activation state of each microglial cell based on the size of the soma and a total size of the at least one projection identified for the microglial cell.2. The method of claim 1 , wherein each soma has an area claim 1 , wherein each projection has a length claim 1 ...
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