17-05-2018 дата публикации
Номер: US20180135079A1
Принадлежит:
Polysaccharide nanoparticles that are particularly useful in for example drug and agent delivery, tissue-specific targeting, for medical imaging and diagnosis, as well as modifiers of physico-chemical properties. The nanoparticles can be highly-branched glucose homopolymers and can be characterized by a uniform spherical shape. They are monodisperse, hydrophilic and produce low solution viscosities. The nanoparticles are non-toxic, biocompatible and biodegradable. Also, the process of isolation of said polysaccharide nanoparticles from various organisms including, but not limited to, microorganisms such as bacteria and yeasts. Also provided are methods for chemical conjugation of the polysaccharide nanoparticles with various agents. Also provided are examples of use of the polysaccharide nanoparticles and their derivatives as drug delivery systems and fluorescent diagnostics. 1. A method of producing a composition of spherical nanoparticles consisting essentially of a homopolymer , wherein the homopolymer is an α-D-glucose with 1→4 linkage and branching points occurring at 1→6 and with a branching degree of 6% to 13% , and a diameter ranging from 20 to 350 nm and an average molecular weight Mw ranging from 2.00×10to 25.00×10Da , and wherein the composition has a polydispersity index (Mw/Mn) value between 1.000 and 1.300 , the method comprising:i. obtaining a biomass,ii. disintegrating cells in the biomass;iii. obtaining a cell lyzate by separating insoluble cell components;iv. removing proteins, nucleic acids, and lipopolysaccharides from the cell lyzate to obtain a crude extract containing polysaccharides; andv. purifying polysaccharide nanoparticles from the crude extract using one or more of ultrafiltration, dialysis, size exclusion chromatography and ultracentrifugation.2. The method of claim 1 , wherein the polysaccharide nanoparticles are purified in step v using ultrafiltration.3. The method of claim 2 , wherein in step v claim 2 , impurities having a ...
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