18-06-2015 дата публикации
Номер: US20150166628A1
Принадлежит:
The present invention relates to compositions, methods and kits for delivery of an IGF-1 protein or a functional fragment or variant, derivative thereof to cells or tissues that express proteoglycans. More specifically, the present invention relates to fusion proteins comprising a heparin binding protein (HB) comprising SEQ ID NO: 1 or SEQ ID NO: 2. Other aspects relate to use of a HB-IGF-1 fusion proteins in methods to deliver IGF-1 to the cartilage for the treatment of a cartilage related disease and disorder in a subject. 1. A composition comprising a HB-X fusion protein , wherein HB is a heparin binding peptide having the amino acid sequence (MKRKKKGKGLGKKRDPSLRKYK) (SEQ ID NO:1) or (KRKKKGKGLGKKRDPSLRKYK) (SEQ ID NO:2) , and X is Insulin-like growth factor 1 (IGF-1) , or functional portion , variant , analog , or derivative thereof.219.-. (canceled)20. The composition of claim 1 , wherein the IGF-1 is a human IGF-1.212. A method of treating a cartilage-related condition comprising administering to a subject an effective amount of a fusion protein of or .22. The method of claim 21 , wherein the cartilage-related condition is a articular cartilage defect including rupture or detachment claim 21 , a meniscal defect including a partial or complete tear claim 21 , Osteoarthritis claim 21 , Traumatic cartilage rupture or detachment claim 21 , Ankylosing spondylitis claim 21 , Capsulitis claim 21 , Psoriatic arthritis claim 21 , Rheumatoid arthritis claim 21 , Systemic lupus erythematosus claim 21 , Juvenile idiopathic arthritis claim 21 , or X-linked hypophosphatemic rickets.23. The composition of claim 1 , wherein the IGF-1 comprises the amino acid sequence of SEQ ID NO: 4 claim 1 , 5 claim 1 , 6 or 7.24. The composition of claim 1 , wherein the IGF-1 comprises the amino acid sequence of SEQ ID NO: 4 claim 1 , 5 claim 1 , 6 or 7.25. The composition of claim 1 , wherein the HB is fused to the N-terminus of IGF-1.26. The composition of claim 1 , wherein the HB is ...
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