31-05-2018 дата публикации
Номер: US20180148670A1
The present disclosure relates to a detergent composition, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions comprising subtilase variants. The present disclosure also relates to the use of said detergent compositions in a cleaning process such as laundry or hard surface cleaning. The present disclosure further relates to a method for removing a stain from a surface, which comprises contacting the surface with the detergent composition. 1. A detergent composition comprising subtilase variants comprising a set of alterations selected from the group of:(a) X167A+R170S+A194P and one or more substitutions selected from the group of X59D, X62D, X76D, X104T, X120D, X133P, X141N, X156D, X163G, X209W, X228V, X230V, X238E, X261D, and X262E;(b) *99aE and one or more substitutions selected from the group of X21D, X59D, X101H, X120D, X156D, X163G, X194P, X195E, X209W, X238E, X256D, X261D, and X262E;(c) X62D and one or more substitutions selected from the group of X101H, X104T, X156D, X163G, X170S, X170L, X209W, X238E, X245R and X262E;(d) X62D+X245R+X248D and one or more substitutions selected from the group of X156D, X163G, X163K, X170S, X209W, and X262E;(e) X170L, X170N, X170S and one or more substitutions selected from the group of X57P, X167A, X172E, X206E; and(f) X99D and one or more substitutions selected from the group of *97aN, *98aA, X98T, X261D, and X262Q;wherein(i) the positions correspond to the positions of the polypeptide of SEQ ID NO: 2;(ii) the variant has protease activity; and(iii) the variant has at least 60%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 1.2. The detergent composition of claim 1 , wherein the subtilase variantcomprises the alterations X167A+R170S+A194P and one or more substitutions selected from the group of X59D, X62D, X76D, X104T, X120D, X133P, X141N, X156D, X163G, X209W, X228V, X230V, X238E, X261D, and X262E.3. The detergent composition of claim 1 , wherein ...
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