Process technology for removing anthraquinone compounds out of wastewater through phanerochaete chrysosporium mycelium

01-01-2014 дата публикации
Номер:
CN103482772A
Автор: Zhang Zhicai
Принадлежит: Jiangsu University
Контакты:
Номер заявки: 40-10-20135890
Дата заявки: 09-09-2013



[1]

The invention discloses process technology for removing anthraquinone compounds through phanerochaete chrysosporium mycelium and relates to the field of bioengineering. Phanerochaete chrysosporium is used as a starting strain, phanerochaete chrysosporium mycelium is obtained by steps of liquid shake-flask culture and liquid mycelium expansion culture, and the mycelium is used for removing the anthraquinone compounds out of wastewater. By adopting the process technology, the removal rate of the anthraquinone compounds can reach 70-100%.



1. A yellow Phanerochaete Chrysosporium degradation anthraquinone compound technology, which is characterized in that in accordance with the following steps: to yellow Phanerochaete Chrysosporium as the starting strain, of test-tube enlarged culture, liquid shake flask culture, cultured bacteria liquid expansion, centrifugal collect the thalli, in the waste water is then used to remove the strains anthraquinone compound.

2. A yellow Phanerochaete Chrysosporium degradation anthraquinone compound technology according to Claim 1, is characterized in that the bacterium is used for yellow Phanerochaete Chrysosporium arbitrary a strain of bacterium.

3. A yellow Phanerochaete Chrysosporium degradation anthraquinone compound technology according to Claim 1, is characterized in that the waste water is applicable to any one of the anthraquinone compound-containing waste water, such as fermentation production waste liquid anthraquinone compound, extracted from plants or waste liquid anthraquinone compound to anthraquinone compound as the raw material to synthesize waste liquid of other products.

4. A yellow Phanerochaete Chrysosporium degradation anthraquinone compound technology according to Claim 1, characterized in wherein the test tube to expand the expansion of the culture in culture medium of the potato sucrose the culture medium.

5. A yellow Phanerochaete Chrysosporium degradation anthraquinone compound technology according to Claim 1, characterized in wherein said liquid shake flask culture in liquid and thalli of expanded liquid shake flask culture medium and liquid cell culture medium are: glucose 5-30 g/L, peptone 0-5 g/L, yeast extract 0-5 g/L, magnesium sulfate 0.2-1 g/L, tween -80   0.5-10 g/L, potassium dihydrogen phosphate 2-9 g/liter, pH5-8,120-140 the sterilizing [...] 20-40 minutes.

6. A yellow Phanerochaete Chrysosporium degradation anthraquinone compound technology according to Claim 1, characterized in wherein the flask culture process conditions for, inoculation 3-5 Phanerochaete Chrysosporium yellow thalline test tube inclined (5 × 5 mm) for a 40-120   ml of culture medium of the 250 ml triangular flask, in the rotational speed: 150 RPMs, the 25-35 [...] , culture 24-72 hours.

7. A yellow Phanerochaete Chrysosporium degradation anthraquinone compound technology according to Claim 1, characterized in wherein the culturing process is, by 1-20% (volume ratio) of the shake flask culture inoculum to inoculate the seed bacteria culture process is the expansion of the liquid, at the temperature the 25-35 [...] , agitation speed 50-200 RPMs, the ventilation amount is 0.2-2:1 minute/volume/volume (breather gas volume/fermentation fluid volume /minute), culture 18-72 hours; culture solution after 3000-6000rpm centrifugal 5-20 minutes, yellow Phanerochaete Chrysosporium strains obtained.

8. A yellow Phanerochaete Chrysosporium degradation anthraquinone compound technology according to Claim 1, characterized in the waste water of thallus removal of the anthraquinone compound: the above-mentioned yellow Phanerochaete Chrysosporium thalli and containing 0.05-1 g/liter of waste water anthraquinone compound (through vacuum concentration or dilution adjusting anthraquinone compound concentration) according to 1:10-50 (mass/volume) mixed, at the temperature the 25-60 [...] , agitation speed 50-200 RPMs, the ventilation amount is 0.2-2:1 minute/volume/volume (breather gas volume/fermentation fluid volume /minute), reaction 3-15 hours, can remove anthraquinone compound.