Method for preparing chitosan with ultrahigh deacetylation degree from euphausia superba as raw material
The invention provides a method for preparing chitosan with ultrahigh deacetylation degree from euphausia superba as a raw material. The method sequentially comprises the following steps: removing mineral substances, removing proteins and lipids, removing mineral substances, removing proteins and lipids, deacetylating and purifying. According to the method, the euphausia superba is taken as the raw material, and the prepared chitosan has few impurities and a high deacetylation degree, and can be applied to the fields of medicines and biology. 1. A in the Antarctic Krill as raw materials of ultra-high deacetylation degree of the chitosan preparation method, characterized in that comprises the following steps: (1) in addition to mineral: heating drying Antarctic phosphorus shrimp shell, to soak in the acid solution, filter, collect the shrimp shell, water to neutral, get the mineral of the shrimp shell; (2) in addition to protein and lipid: step (1) of the shrimp shell in addition to mineral after heated in the lye, then filter, collect the shrimp shell, water to neutral, get the protein and lipid of the shrimp shell; (3) in addition to mineral: the step (2) in addition to protein and lipid of the shrimp shell after soak in the acid solution, filter, collect the shrimp shell, water to neutral, get the mineral of the shrimp shell; (4) in addition to protein and lipid: the step (3) of the shrimp shell in addition to mineral after heated in the lye, filter, collect the solid, the solid water to neutral, dried to obtain chitin; (5) deacetylation: step (4) by adding concentrated alkali chitin obtained by heating to reflux in, filtering after cooling to obtain the solid, the solid water to neutral, dried to obtain crude chitosan; (6) purification: step (5) the obtained crude chitosan is soluble in dilute acid get mixed solution, a mixed solution with dilute alkali the titrate states pH value to 8-8.5, washed and dried to obtain bright white powder, in other words chitosan. 2. Process for preparing chitosan according to Claim 1, characterized in that step (1) in, the drying the Antarctic Krill shell on the concentration of the acid solution 0.05g/mL -0.1g/mL. 3. Process for preparing chitosan according to Claim 1, characterized in that steps (1) and step (3) in, the acid is 2mol/L hydrochloric acid, at room temperature operation, as the soaking 1h. 4. Process for preparing chitosan according to Claim 1, characterized in that steps (2) and step (4) in, said lye is 1mol/L of sodium hydroxide, the heating operation as a heating boiling 1h. 5. Process for preparing chitosan according to Claim 1, characterized in that steps (5) in, the adding the concentrated alkali in saline, in 110 the heating to reflux [...] 1h, said concentrated alkali as the mass fraction is 50% of sodium hydroxide solution. 6. Process for preparing chitosan according to Claim 1, characterized in that steps (5) the rear, the crude chitosan uses steps (5) method of re-deacetylation. 7. Process for preparing chitosan according to Claim 1, characterized in that step (6) in, the crude chitosan dissolved in 0.01g/mL of get mixed solution in acetic acid, using 0.1mol/L the titrate states mixed solution of sodium hydroxide to a pH value of 8.5.