Preparation method of pig spleen transfer factor
The invention provides a preparation method of a pig spleen transfer factor. The preparation method comprises the steps of tissue mincing, breaking by homogenizing, microfiltration, ultrafiltration and the like. A high-pressure homogenizer is adopted to rapidly break cells and can effectively keep the activity of the transfer factor; besides, compared with the prior art, the technical scheme provided by the invention does not need a freezing-thawing step, therefore, the production period is shortened; the microfiltration-ultrafiltration technology is adopted to intercept effective constituents with appropriate molecular weight, so as to obtain a transfer factor stock solution, and compared with the traditional dialysis technology, the microfiltration-ultrafiltration technology is relatively high in efficiency. The method provided by the invention is suitable for scale production requirement, and the production period can be greatly shortened; the transfer factor solution prepared from the healthy pig spleen as a raw material through defatting, cell breakage, microfiltration and ultrafiltration is non-specific immunoregulator and is used for enhancing the immunological function of animal bodies. 1. A method for preparing artemin transfer factor, comprising the following steps: 1) a Pig splenetic the chopped meat grinder, by specifically involves Norcantharidin of minced meat, the meat of the total mass of meat emulsion is added in 0.1-0.5 times of purified water, by grinding the colloid mill, is added to the purification and then continue to direct to this step the total quality of the purified water of the total mass of the meat 1.4-1.6 times, mix, get the homogenate for use; 2) use of the high-pressure homogenizer to the step 1) to the homogenizing treatment of the homogenate, inactive; 3) step 2) homogeneous treated homogenate and purified water to 1 : 0.2-1: 0.6 (w:v) mix proportion, for obtaining slurry mixture; 4) use of microfiltration system, to 0.005-0.5 the aperture m in step 3) the slurry mixture to microfiltration, for collecting filtrate; 5) use of an ultrafiltration system, to 4-10KD aperture in step 4) the obtained filtrate is ultrafiltration, collecting filtrate is transfer factor stock solution. 2. A method for preparing artemin transfer factor according to Claim 1, characterized in that step 1) the Pig splenetic to fresh, removing the ctd and fat Pig splenetic. 3. A method for preparing artemin transfer factor according to Claim 1, characterized in that step 2) the homogenization treatment process is not higher than the temperature of the slurry in the 40 [...]. 4. A method for preparing artemin transfer factor according to Claim 1, characterized in that the step 3), step 4), step 5) the temperature of each component and is not higher than the operating temperature of the 35 [...]. 5. Any one of a method for preparing artemin according to Claim 1-4 transfer factor, which is characterized by comprising the following steps: 1) a Pig splenetic the chopped meat, specifically involves Norcantharidin 2 again get meat emulsion, is added to the meat of the total mass of meat emulsion 0.25 times of purified water, by grinding the colloid mill, and then to the total mass of meat emulsion wherein the added 1.25 times of purified water are mixed, is made for use of the homogenate; 2) use of the high-pressure homogenizer to the step 1) to the homogenizing treatment of the homogenate 2 times, for use; 3) step 2) homogeneous treated homogenate and purified water to 1 : 0.4 (w:v) mix proportion, for obtaining slurry mixture; 4) use of microfiltration system, to 0.1 the aperture m in step 3) the slurry mixture to microfiltration, for collecting filtrate; 5) use of an ultrafiltration system, to 6KD aperture in step 4) the obtained filtrate is ultrafiltration, collecting filtrate is transfer factor stock solution.