Fast method for obtaining genetically homogenized wheat powdery mildew
The invention discloses a fast method for obtaining genetically homogenized wheat powdery mildew. A single conidium is picked by a specially-made teasing needle and transferred onto an in-vitro leaf section under a stereo microscope. The leaf section is put in a culture dish containing benzimidazole (50ppm) preservative matrix, more than one spore chain is formed after light and dark alternative culturing for seven days, and powdery mildew piles with the diameter of about 0.5cm are formed after 17 days. At least three circulations are reduced in the one-time technological flow of the pure strain compared with the common purification technology, and 20-30 days can be saved. The strain obtained by adopting the method is entirely genetic homogeneous, and provides material guarantee for the follow-up study related to wheat powdery mildew. 1. Obtaining a genetic homogeneity wheat powder oxysporium shortcut method, characterized in that under the stereomicroscope, uses a special needle picking single conidia exsomatize leaf section on the; leaf section in a bath containing 50 PPM benzimidazoly fresh-keeping substrate in the culture dish, (17 ±1) under °C, dark light alternated culture 7d to a is formed after spore chain , 17d to form a diameter of about 0.5 cm the powder mildew piles of. 2. Swift method according to Claim 1, characterized in that the special needle making method is as follows: from about 13 cm of a prod, thickness such as a toothpick or cotton swabs, workover is a smooth cylindrical, after sharpening one end of a slit from, the eyebrow rough and straightforward in the base is inserted into the gap, leaving 2 mm tip, or all-purpose adhesive for fixing the transparent adhesive tape base for a prod the eyebrows.