Process for preparing vancomycin loaded/hydroxyapatite artificial joint prosthesis
The invention discloses a process for preparing a vancomycin loaded/hydroxyapatite artificial joint prosthesis. The process comprises the following steps: firstly, preparing a titanium dioxide bio-membrane which is porous in surface and closely combined with a matrix on the surface of an artificial joint prosthesis by a micro-arc oxidation technology; then, generating a vancomycin loaded/hydroxyapatite composite antibacterial bio-membrane on the porous surface of the titanium dioxide bio-membrane by adopting a simulated body fluid technology. In the composite antibacterial bio-membrane, the inner layer is made of compact titanium dioxide, the outer layer is of a porous structure, and vancomycin loaded hydroxyapatite is filled in the porous structure. According to the process, the vancomycin loaded/hydroxyapatite composite antibacterial bio-membrane is prepared on the surface of an artificial joint prosthesis by adopting a two-step method, the composite antibacterial bio-membrane in-situ grows from the surface of the artificial joint prosthesis, and is combined with the matrix in an interdigitating manner, so that the vancomycin loaded/hydroxyapatite artificial joint prosthesis has high combining strength, the bio-activity of artificial joint prosthesis can be remarkably improved, the sustained-release of vancomycin has a good antibacterial effect, and the artificial joint prosthesis has a very important meaning in preventing prosthesis infection after artificial joint replacement. 1. A vancomycin/hydroxyapatite preparation process of artificial joint prosthesis, characterized in that the specific steps are as follows: Step one, artificial joint prosthesis surface porous titanium dioxide biological membrane preparation: Preparation of micro-arc oxidation electrolyte solution, after sanding and polishing the surface of the artificial joint prosthesis in the electrolyte is immersed in the micro-arc oxidation, to artificial joint prosthesis as anode, to the cathode stainless steel as micro-arc oxidation, the whole process of the controlling the temperature of the electrolyte in the 30 the following [...] , oxidation time is 5-30min, the sample is taken out after the reaction, the surface of the artificial joint porous surface into the biological membrane of titanium dioxide, using deionized water to clean flushing, drying the spare. 2. Stated, load banko ycin/hydroxyapatite preparation of artificial joint prosthesis: SBF bionic artificial simulated body fluid is prepared, in accordance with added 60 mg/100 ml of vancomycin, after the above-mentioned micro-arc oxidation treatment of the artificial joint prosthesis implantation of containing vancomycin in SBF solution, in the 37 in the constant-temperature table [...] slow oscillation, be replaced once every two days and SBF vancomycin, after two weeks, natural air drying, can be generated on the surface of the artificial joint in banko ycin/hydroxyapatite composite antibacterial biomembrane. 3. Load banko ycin/hydroxyapatite preparation process of artificial joint prosthesis according to Claim 1, characterized in that The artificial joint prosthesis material is Ti-based metal. 4. Load banko ycin/hydroxyapatite preparation process of artificial joint prosthesis according to Claim 1, characterized in that Step one of the method for preparation of micro-arc oxidation electrolyte for: the 0.01mol/L sodium hexametaphosphate and 0.1mol/L of sodium hydroxide dissolved in deionized water, fully stirring mixing, standing 60 min after spare. 5. Load banko ycin/hydroxyapatite preparation process of artificial joint prosthesis according to Claim 1, characterized in that Step one of the micro-arc oxidation process parameters :: voltage 200V-360V, the electric current density is 3-6A/dm2, duty ratio is 10-30%, frequency is 500-600HZ. 6. Load banko ycin/hydroxyapatite preparation process of artificial joint prosthesis according to Claim 1, characterized in that Step two of the method for preparing SBF in 800 ml distilled water added depends on NaCl 7.996g, NaHCO3 0.350g, KCl 0.224g, K2 HPO4 · 3H2 O 0.228g, MgCl2 · 6H2 O 0.305g, CaCl2 0.278g, Na2 SO4 0.071g, used 50mmol/L slow hematic acid amine (CH2OH) · 3CNH2 and 0.1MHCl buffer pH value is 7.35-7.45, constant volume to 1000 ml. 7. Load banko ycin/hydroxyapatite preparation process of artificial joint prosthesis according to Claim 1, characterized in that the composite antibacterial of the biomembrane of the inner layer is a dense titanium dioxide, the outer layer is a porous structure, the porous structure in the hydroxyapatite of vancocin.