Rhizoma bletillae industrialized seedling method

Technical Field

The invention relates to a field of agricultural science and technology, in particular to a kind of white and factory method of growing seedlings.

Background Art

White and bitter, astringent, flavor. Convergence hemostasia, detumescence function. Used for the treatment of the lung empty for a long time coughs , haemoptysis, hematemesis, homafecia, nosebleed, traumatic hemorrhage, ulcer stiring, scald and skin and cracked, curative effect is better, market demand gradually expanding. Furthermore white and in cosmetic, industrial businesses also a demand. White and from 1998 years without failure has been popular, the demand, a continued rise in prices, many local valuable goods. The temptation of the lower, people have wild resources reservely, wild white and resources are depleted, the shortage of supply. Because the traditional plant is tuber sub-strain propagation, the propagation coefficient is low, but also white and growth long service life up to 3-5 years, the source is not in the short-term, artificial planting difficult to get-scale. White and capsule contains tens of seed, seed breeding using tissue culture technology, is an effective way to white and sources.

Content of the invention

The invention the technical problem to be solved is to: provide a white and factory method of growing seedlings, survival rate can be up to 91% or more, the operation is simple, low cost, high reliability, solves the problem of white and germchit reproduces difficultly , to realize the white and the factory seedling.

The invention is realized in the following way: white and factory method of growing seedlings, comprising the following steps:

1) propagating the garden preserves seed : good white screening and are planted in the propagation plants the garden , and artificial pollination, treats white and method of harvesting from yellow to brown when the, selection of full yellow the cracking of a capsule;

2) before Sowing the seed treatment: gather white and capsule under running water after flushing wipes does , dialogue and capsule is sterilized;

3) seeding: seeding culture medium includes 1/2MS culture medium, which contains 30 g/l glucose and 6.5 g/l agar powder; the under aseptic conditions, the seed is uniformly to the surface of the culture medium, then the seeding of the culture bottle timely to the culturing chamber in subdueing;

4) switching: seed germination after 2 months, to begin switching, each bottle switching 15 tree seedling, switching medium comprises medium MS, 1.0 mg/L   NAA, 0.5 g/l activated carbon, 30 g/l cane sugar and 6.5 g/l agar powder, the switching of the culture in the cultivating chamber miao Fang 3 months after the transplantation can be the bottle;

5) building domesticating cotes: selecting fertile land vacuum domesticated awning of the building, and provided with an automatic water spraying facilities, a seedling matrix and the inside of the greenhouse for wide 1.2-1.8m, high 15-20cm the seedbed; laying opening cultivation cavities in the hillside in domesticating casings 65-75% sunshading of;

6) transplanting the bottle: in 3-10 month domesticating transplantation of the tissue, the selection culture 4-6 months, the tissue and a bulb, is placed in the domesticated awning or outdoor shady place, 5-7 cleaning tissue after, the wash clean tissue of the transplanted seedling bed domesticating cotes in, strain is 5 cm × 5 cm, in a timely manner after transplantation pouring originally water ;

7) water and fertilizer management of: the temperature control of the shed domesticating the 20-30 [...] , soil humidity keep in 70-80% ; watering one month after transplantation 1 secondary nutrient solution, every six months after the foliage nutrient solution 1 time, 80-100 days leaves the awning transplanting daejon.

The step 1) in, the tubes will be planted in the flowering stage of the white and isolation, for autocopulation the homophyletic flower, the flower on the same day in the morning is 11 point prior to pollination.

Step 2) the disinfection is, absorbent cotton of alcohol soaked for dialogue and capsule surface wiping 2-5 time, and then the white and capsule is placed in the antiseptic in the culture dish, the on of the blocked alcohol soak clean the epidermis of a capsule the lint ball scratches , used for the disinfection of tweezers to clip a capsule in the alcohol lamp burn quickly back-and-forth outer flame place 2-3 seconds, and prevent the capsule when, so repeated burn 2 time, the capsule of the dialogue and completed.

The steps 3) seeding of the culture bottle is the condition of the chamber: temperature is   25 ±1 °C, illumination time 12h/d, the intensity of illumination 1800-2200   lx.

Step 5) the substrate including the full decomposition of the wood chip, corn straw compost or straw daejon soil, compost and, based on the mass ratio of 3:1:1 to be fully mixed.

The step 7) in, watering one month after transplantation 1 for the nutrient solution nutrient solution 1/10MS a large number of elements, uniformly sprayed per sq m 90-110ml, every six months after the foliage 1 time added to each liter of culture solution of 0.4-0.6 mg of   NAA   1/4MS nutrient solution, for each 100 the foliage of seedlings 45-55ml.

Due to the adoption of the above-mentioned technical scheme, the invention has fully utilized the capsule and the advantage of the large number of the seeds, the seeds to explant, the establishment of the white and tissue culture propagation technique and technology domesticating tissue, carried out under aseptic conditions germination growth, has overcome the external environment the impact of the dialogue and seed germination, greatly improves the white and seed germination rate and the growth speed, 5 months bulbs can be grown from seeds diameter of 0.7 cm of the left and right of the plant, only the middle of switching 1 time, the industrial production of white and germchit, the operation is simple, low cost, high reliability, the whole seedling cycle about 8 months, domesticating survival rate can be up to 91% or more. After domesticating 80-100 day white and seedling direct transplantation daejon has comparatively high survival rate, up to 98% the left and right, 4-5 years can be harvested after, solves the problem of white and germchit reproduces difficultly , to realize the white and the factory seedling. The invention is simple and easy, low cost, good use effect.

Mode of execution

The embodiment of the invention is 1: white and factory method of growing seedlings, comprising the following steps:

Test location: Guiyang guizhou NIENBURG

Test time :-25 November 2012 2014 years 12 month

Test material: for test species is white and, from guizhou zhenyuan Countyqingxi Town acquisition.

Test method:

1) propagating the garden preserves seed : 25 November 2012, a healthy white of the insect pest and disease large tubers planted in guizhou Nyasaland NIENBURG resources. 4 month during bloom insect-proof net for isolation is good, preventing insect string powder ; used in flowers open tweezers picking anther on the same day, on the open end of the head, in the morning on 11 point before pollination; after pollination 5 months capsule about treats from yellow to brown when started, selecting the apparent full, of relatively hard shell capsule harvesting to cracking; filled capsule used kraft paper envelope, Allochroic silica gel is dried in for 5 days, then by placing 4 the refrigerator cold storage [...] spare;

2) before Sowing the seed treatment: seed after Sowing in a timely manner. Before seeding, the capsule and the flushing clean surface under running water of mud and sand, moisture dry with filter paper, soaked absorbent cotton of alcohol for dialogue and capsule surface wiping 2-5 time, and then the white and capsule is placed in the antiseptic in the culture dish, the on of the blocked alcohol soak clean the epidermis of a capsule the lint ball scratches , used for the disinfection of tweezers to clip a capsule in the alcohol lamp burn quickly back-and-forth outer flame place 2-3 seconds, and prevent the capsule when, so repeated burn 2 times, that is, complete disinfection treatment of white and of a capsule;

3) seeding: seeding culture medium is configured in advance, the seeding culture medium in the culture medium is the basis for 1/2MS, comprising 30 g/l glucose and 6.5 g/l agar powder; the under aseptic conditions, using scalpel to the white and ventage capsule cut, the seed is uniformly to the surface of the culture medium, each bottle is Multicast 150 grain and seed, the seeding of the culture bottle timely to the culturing chamber in subdueing; seeding of the culture bottle is the condition of the chamber: temperature is   25 ±1 °C, illumination time 12h/d, the intensity of illumination 2000   lx.

4) switching: seed germination after 2 months of culture medium replacement in time, begin to switching, selection stockier, the color of the fresh, each bottle switching 15 seedling tree, the root of the seedling is planted in shallow gently switching medium, the formulation of the switching medium culture medium MS, 1.0 mg/L   NAA, 0.5 g/l activated carbon, 30 g/l cane sugar and 6.5 g/l agar powder, the switching of the culture in the cultivating chamber miao Fang 3 months after the transplantation can be the bottle;

5) building domesticated awning and preparing seedling matrix: NIENBURG guizhou for intelligent greenhouse as domesticated awning , 10 March 2014 will be applied to the soil of each mu of 1500 kg of corn straw compost turning. The organic solvent for sawdust, corn straw compost and daejon soil, based on the mass ratio of 3:1:1 as a seedling matrix is fully mixed. A seedling matrix for wide and 1.5m, long 20 meters, high 15-20cm of the seedbed. The bed control the moisture content of 70%. In laying opening cultivation cavities in the hillside domesticating casings 70% sunshading of;

6) transplanting the bottle: and 5 March 2014 3 month 17 day, the selection culture 4-6 months, and tame corm tissue transplant, outdoor shady before the transplantation is the first 5 days, then cleaning tissue, the tissue will wash the seedling bed domesticating cotes in, the bulbed buried layer when replanting 1-2cm, strain is 5 cm × 5 cm, in a timely manner after transplantation pouring originally water ;

7) water and fertilizer management of: the temperature control of the shed domesticating the 20-30 [...] , the temperature of which exceeds the 25 to [...] spray cooling. Seedling matrix humidity keep in 70-80% ; watering one month after transplantation 1 time nutrient 1/10MS a large number of elements, uniformly sprayed per sq m 100 ml, every six months after the foliage 1 time per liter of adding 0.5 mg of the   NAA 1/4MS nutrient solution, for each 100 the foliage of seedlings 50 ml. 2 months after transplant the seedling into the survival rate of the two groups are respectively to 91.8% and 93.1%.

8) daejon transplanting: 29 May 2014 and 6 months 10 day survival of the shed domesticating two groups of white and seedling transplanting daejon, the row spacing 20 cm, is 30 cm. Aglaonema the sheathes in a timely manner, and using 70% sunshading shade, 1 month after transplantation of respectively investigated the survival rate of 98.4% and 97.8%.

The above is the only way the preferred embodiment of the present invention, the invention is not limited to the above embodiment. Understandable technical personnel in this field without departing from the invention spirit and the concept of directly derived or Lenovo on the premise that other improvements and changes to, included in that the scope of protection of this invention.