PREPARATION METHOD OF MASK PACK SHEET USING STYELA CLAVA SKIN AND NATURAL POLYMER
The present invention refers to a cosmetic composition including america compared to virtue shell and natural polymer active ingredient are disclosed. After the mask is formed by cutting off the air dust, heat, characterized but difficult in such passage, the hair follicle casing has pores and skin expansion with circulation blood through the skin is installed immediately after the grafting rate of metabolism react on each other. The initial step is blood circulation and peeling skin care and primarily use, made of paraffin used, gypsum etc.. The pack comprises a whole blocking heat, moisture, carbon dioxide passes pivotably. Hereinafter active ingredient for supply and, due to capillary vasoconstriction and skin as the temperature is decreased, and a stand-alone number and force of the moisturizing skin sebum and dead keratin, hair follicle resulting in contraction of the pores and helps. The skin care used for late stage which, powder, emulsion, cream form number it becomes width, directional keys. In terms such as using our normal mask and pack and, packs mask pack function such as a has a plurality of hierarchies. Disconnecting mask pack is a skin on the surface skin from another form with the outside skin surface are shut off. The rise in the temperature in the surface of the skin is accelerated by moisture evaporation is number of formular I billion blood circulation effects can be achieved. Film-forming pores by adsorbed on the skin surface or the number remaining in volatile impurities and volatile waste and waste from the mask packmask pack number, the wetting ability to soften the pack number encoded number of the control dead stratum corneum wetting ability. A coating film containing moisture and moisture evaporation is mask pack and number of pores in the stratum corneum by one billion expands and extending mask pack resulting due to non-phagocytic of active ingredient contained in the line. Is configured to remove the type mask pack market quality and is characterized in that the laundry is focused needs of people and functional, premium number article are variously control signal in the nanometer range. The upper 2000's openingmask pack classification is applied on the skin of a kind in which began to coming into single categories. In particular cosmetic active ingredient selected from the group such as non-woven fabric sheet as a medium for transmitting in response from the two half the generation 1 generation 2, 3 generation hydrogel, 4 generation wet bio cellulose, etc. 5 dry bio cellulose leading from the power generation. paper mulberry stalk skin whitening, anti-aging, skin regeneration, cosmetic composition effective for treating atopic skin disease habitual use off and, hygroscopic, deodorant, breathable, etc. that excels in antibacterial mask pack also includes using the paper type. The number is a number paper mulberryit is sour with the mote but Oh billion 'car play F (Kazinol F)' component as a pack of disclosed. The fact F paper mulberrycar attendant with the odor of whitening efficacy of selected from the group consists of immersing a sheet number under public affairs directly on skin whitening efficacy of functional sheet could be bonded each other. For example made by others unusual white paper from hand of a human being that when making a general paper compared with while preventing soil, without discoloration of the Korean paper is made using a discoloration preventive number paper mulberry also well not even that of whitening paper mulberry well shown disclosed. C melasma site specific phenomenon appears only decodes the number paper mulberrycar melanin and is playing. An excellent effect and inhibit it is sour with the mote but Oh number, this number is known as good an effect of inhibiting it is sour with the mote but Oh walk more isethionate. Α - tocopherol than is car play E, car play A lung rule it buys (ferulic acid) is superior antioxidant activity have shown. Potent inhibition activity against number it is sour with the mote but Ohcar play E antioxidant activity having result, IC50 is 241. The vitamin C 3 μm have shown better it is sour with the mote but Oh number inhibitory activity. In yet paper mulberrycar play A are determined structure includes a pulse, these antioxidant ingredients are enhanced by removing free radicals as well as anti-aging and in-vivo vitality, it is sour with the mote but Oh number inhibitory activity due to excessive dye prevents deposition disease through whitening number and may be utilized to a lever is coming in now. The melanin synthesis antioxidant ingredient paper mulberry shell of luteolin absolutely free of component is higher in level phenyl pro group toe id series compound compositions for lightening skin billion number and of the same composition contains billion it will carry on shoulder can be prepared in the number larger than white skin have. However, various kinds of mask packpaper mulberrymask pack number used in Korea paper sheet or the bath and terminal of the poly-and, in addition relating to making mask packpaper mulberry Korea paper or sheet yet reported bar is free. Said prior art door such as if the point number, the present invention refers to an active ingredient derived from natural polymer paper mulberryamerica compared to virtue shell and without skin irritation to the skin and, functional beauty can be cut article number number the number under public affairs cosmetic composition intended for high pressure liquid coolant. In order to achieve said purposes, the present invention refers to active ingredient america compared to virtue shell and natural polymer including cosmetic composition number under public affairs substrate. According to the present invention derived from natural polymer for suppressing the paper mulberryamerica compared to virtue shell and skin irritation free, functional beauty can be cut article number number high pressure liquid coolant. In one embodiment of the present invention also (a) and Figure 1 shows a high pressure liquid coolant along a number indicating fine powder (b) shell america compared to virtuepaper mulberry shell fine powder are disclosed. Figure 2 shows a fine powder content of high pressure liquid coolant along a different number america compared to virtue shell also in one embodiment of the present invention indicating mask pack sheet are disclosed. Fine powder amount of 5% (w/w) agar america compared to virtue shell 2a is also added and are, 2b is added 10% (w/w) are disclosed. Figure 3 shows a moisture absorption representing the result of measurement according to one embodiment of the invention agar content according to the invention are disclosed. Figure 4 shows a representing the result of measurement according to the invention retains the content agar according to one embodiment of the invention are disclosed. 4a is also tensile strength, elongation is indicating 4b are disclosed. Figure 5 shows a shell according to the invention according to one embodiment of the invention moisture content america compared to virtue absorption representing the result of measurement are disclosed. Figure 6 shows a shell according to the invention retains the america compared to virtue content according to one embodiment of the invention representing the result of measurement are disclosed. 6a is also tensile strength, elongation is indicating 6b are disclosed. In one embodiment of the present invention representing the result of measurement of cytotoxicity mask packamerica compared to virtue shell powder content along a according to Figure 7 shows a also are disclosed. Hereinafter the present invention that are directionally-described substrate. In the present invention paper mulberry and dehydrating agar-agarmask pack sheet number using high pressure liquid coolant to be performed on all natural polymer fine powder to be established when the juice and each step reaction conditions, a natural carboxylic height difficulty of potato starch, agar for food, such as whether to quiet crosslinking or gelling number number number tightly the grass biometric by acrylation mask pack sheet number and high pressure liquid coolant, through mask pack prepared by the number of his relaxation properties. The present invention refers to such active ingredient including cosmetic composition number under public affairsamerica compared to virtue shell and natural polymer substrate. paper mulberry said natural polymer is preferably derived from natural polymer, more preferably paper mulberrypaper mulberry shell powder obtained by grinding and then drying shell are disclosed. In addition to the active ingredient of the present invention commonly used cosmetic composition said cosmetic composition number antioxidant, stabilizing number, number solubilized, vitamin, pigment, such as perfume and a carrier can be a conventional auxiliary number is further included. For example, said cosmetic composition glycerin, butylene writing this call, [...] polyoxyethylene hardened castor oil, tocopheryl acetate, sheet [...], petal reel glow, [...], sodium citrate, alane of auxiliary component can be further examined. Cosmetic composition of the present invention is applied to skin essentially three, with reference to the even number the party industry cosmetic composition typically any number type number 1308. bath tank. For example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, number - surfactants containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray or the like can be elder brother anger number but, limited to are not correct. More particularly, flexible skin, nutritional skin water, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, mask pack, spray or wet-number number bath 1308. Of the present invention number type paste, cream or it is a gel carrier ingredient when animal oil, include esters of fatty acids, wax, paraffin, starch, coating [...], cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide and the like can be. This powder or spray when a carrier component of the present invention number type lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like can be, in particular spray case additionally chloro [...], propane/butane, dimethyl ether can be such as to expel the projectile. The solvent solution or emulsion of the present invention number type when the carrier components, solubilized number, which can be like device for number, specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3 - butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, sorbitan fatty acid esters can be like. If the carrier components of the present invention number type suspension water, ethanol, propylene glycol liquid such as dilution number; box-ethoxylated alcohol, a polyoxyethylene sorbitol ester, a polyoxyethylene sorbitan ester or the like number; microcrystalline cellulose, aluminum meta [...], bentonite, agar, [...] coating can be like. The interface type of the present invention number when a carrier ingredient containing cleansing - active number aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfonyl succinic ester, cleanser, imidazole [...] derivatives, methyl [...], cooperates with the disadvantages, fatty acid amide ether sulfate, alkyl polyglycoside, aliphatic alcohol, fatty acid glyceride, fatty acid d ethanol amide, include esters of fatty acids, the induction body which it teases, ethoxylated glycerol fatty acid ester can be like. In the embodiment hereinafter described more specifically with respect to the example but in the present invention. In the embodiment of the present invention is described for purposes of protection range is relayed only these number that is even endured. In the embodiment 1. Number of tank shell powder america compared to virtuepaper mulberry shell powder The gyeongsangnam-doamerica compared to virtue shell assembly has such as a dyeing machine for high pressure collecting at a dry shell 1% to 1% every other number on the hypochlorous acid soda oh it is an oxygen etherification to H and the temperature for 95 °C after bleaching2 O2 A transparent conductive layer with an aqueous solution is cooled to 10% and 1% acetic acid added was added 60 °C neutralization. After taking into account the number of stand-alone number impurities work percentagemask pack his bleaching white color. And neutralize treated america compared to virtue shell dehydrator and road through complete very dry. Preprocessing america compared to virtuepaper mulberry shell and fully effective in reducing shell, Pin milling M/C (Daehaw, Korea) twice through (40mesh, 120mesh) by grinding in the process of fine powder was used. As also shown in (a) and 1 each other and washed paper mulberry shell fine powder, fine powder (b) confirm the lumping america compared to virtue shell free queue. In the embodiment 2. mask pack sheet number bath The films used in experiment 1% (w/v) of Agar and 0. In a solution of 1% (w/v) of fine powder dispersion amount of 5% (w/w) Agar [...]america compared to virtue shell, according to the concentration of 10% (w/w) fine powder added america compared to virtue shell absorption, tensile strength, adsorption degree, the water vapor permeability or the like number film was high pressure liquid coolant to apoptosis as follows. First, 100 ml distilled water to Agar (1g, 1% (w/v)) [...] on (0. 1g, 0. 1% (w/v)) a 300 ml function cap is placed, then fine powder amount of 5% (w/w) Agar america compared to virtue shell, added 10% (w/w) heated agitator (hot plate & magnetic stirrer, mS provided 300HS, Misung Scientific, Korea) was 5 minutes in boiling point and mixing using heating. At the time when the solution is then heated to 40 °C are marked on the supply roller shell powder after spray america compared to virtue[...] square cell (125 × 125 × 20mm) into the basin. Then form gel until more then heated with dryer placed in about 45 °C (fO-a 600M, Jeio Tech, Korea) degree with respect to the day after drying. After drying in a dryer sheet film swelling out water to soak out to his number mask pack high pressure liquid coolant. High pressure liquid coolant to a fine powder amount of 5% (w/w) Agar america compared to virtue shell number mask pack sheet (a) 10% (w/w) added on at a rate of 2 (b) is added mask pack sheet also such as disclosed. In the embodiment 3. According to the moisture absorption measuring agar content According to the moisture absorption measurement Agar content 0. 25%, 0. 5%, 0. 75%, 1. 0% of agar hydrogel weight of complete drying in a dryer state, each sample of immersing a limit absorb distilled water at room temperature until the coating method using the constant time intervals after excessive holes number is accomplished through weighing 1 to equations using a stand-alone was calculated. [Mathematical equation 1] Absorption (%)=[(Ws - Wd )/Wd ] × 100 Wherein, which includes a film Wd dry weight, substitute film weight Ws is are disclosed. Each absorbance prevents the total 3 times after repeated experiments by average calculated, also shown to result 3. As shown in fig. 3, the higher the concentration of lower moisture absorbance agar is also used for capable of. However agar concentration of certain criteria or more gelled layer is formed is not, been considered to properly reduced in concentration of 1% agar gel comprising suitable line in degree. In the embodiment 4. According to measuring retains the agar content For the measurement of 0 retains Agar according to content. 25%, 0. 5%, 0. 75%, 1. 0% of agar hydrogel tensile strength and elongation (UTM sSTM provided 1, United, USA) by using a tensile burglar have, 5 kg load cell was used. The test piece according to ASTM D1708 specification came, when the speed of 10 mm/min at room temperature measured, a test is repeated 7 times after experiment was calculated by total average. As a result also 4 as shown, agar content according to agar hydrogel tensile strength agar content 0. 5% appears to be highest when a starting point, the elongation is also used for 1% agar content appears to be highest when the first call request. In the embodiment 5. The water vapor permeability according to agar content 0. 25%, 0. 5%, 0. 75%, 1. Water vapor permeability based on the ASTM 96 0% of agar hydrogel moisture permeability were measured. 30 ml 50 mm 40 mm diameter cylindrical cup provided with a filling of distilled water sample after material and fixed to an inner diameter size, temperature 37 35 2 °C by 5% relative humidity by a user pipe (fO-a 600M, Jeio Tech, Korea) by a given time interval for measuring the weight along and, after averaging total 3 times been repeated experiments. Vapor expressions calculated according to diagram 2, shown to table 1 to the result. [Equations 2] The water vapor permeability (g/m2/h)=[(Wi - Wf )/24/A] × 106 Wherein, Wi And an initial sample weight, Wf Is of constant weight and of time after, A is area are disclosed. As shown in said table 1, vapor diagram agar content 0. When 75% 35. 80g/m2/hr, 1% agar content when 35. 50g/m2/hr similar value shown to exhibit the highest appeared. Number of results from such results of said in the embodiment 3 and 4 performs numerical control machine having an agar 1% mask pack most suitable to been considered to gelling number concentration. In the embodiment 6. According to the moisture absorption measuring america compared to virtue shell powder content Agar paper mulberry shell on each 1% concentration, 0. 1% signal and generates, through the high pressure liquid coolant absorbancemask pack sheet moisture content of only america compared to virtue shell number were measured. Moisture absorbance 0. 05%, 0. america compared to virtue shells containing 1% by the weight of each sample number a hydrogel state high pressure liquid coolant drier to fully absorb distilled water at room temperature drying of immersing a limit until the constant time intervals after excessive heating and coating method using the number is accomplished through weighing 3 to calculates expressions using a stand-alone, also 5 shown to result. [Equations 3] Absorption (%)=[(Ws - Wd )/Wd ] × 100 Wherein, Wd Which includes a film dry weight, Ws The swollen film weight are disclosed. Each experiment was repeated 3 times after calculated by absorbance prevents the total average. As shown in fig. 5, 0 america compared to virtue shell. 05% and 0. Comparing 1% content when seen, 0. 1% of added hydrogel moisture absorbanceamerica compared to virtue shells cream under the better, since such result america compared to virtue shells hydrophilic cellulose to the source been a lever. In the embodiment 7. According to measuring retains america compared to virtue shell powder content Agar paper mulberry shell on each 1% concentration, 0. 1% signal and generates, through the content of the sheet number mask packamerica compared to virtue shell only determined the retains the high pressure liquid coolant. 0. 05%, 1. 0% america compared to virtue shells containing hydrogel of tensile strength and elongation (UTM sSTM provided 1, United, USA) by using a tension burglar have, 5 kg load cell was used. The test piece according to ASTM D1708 specification came, when the speed of 10 mm/min at room temperature measured, a test is repeated 7 times after experiment was calculated by total average. As a result also 6 as shown, the tensile strength hydrogel america compared to virtue shell content according to a large-invisible but america compared to virtue shell content difference, when america compared to virtue elongation increases is also used for capable of shell content decreases. In the embodiment 8. america compared to virtue shell powder content according to cytotoxic Extract of skin cells to evaluate acrotism organization under bloodpaper mulberryamerica compared to virtue film/an influence on cell lines in Korean cell line bank (Korean Cellline Bank, KCLB) is L-a 929 (subcutaneous connective tissue) as well as seller. (FBS, Hyclone, Logan, UT, USA) 10% L-a 929 cell lines [...]oh serum, Eagle's Minimum Essential Medium (MEM, Hyclone) containing penicillin-resistant Streptococcus pneumoniae and Streptococcus feed (Gibco) using booked to 37 °C, 5% CO2 Him as in incubator conditions. When the daily replacement medium, 80 - 90% with respect to the degree the passaging cells in growth medium. Agar 1g, paper mulberry agar 1g + 0. america compared to virtue 1g + 0. 5g, paper mulberry agar 1g + 0. america compared to virtue 1g + 1. 0g number 3 to a high pressure liquid coolant is film skin cells activity of each film 16 attention for evaluating 200mm 2 behind and well-plates 24 is placed, by dipping each 1 ml MEM medium for cell culture 24 hours behind 37 °C, 5% CO2 Him as in incubator conditions. 24 dipping medium after some time replacement and cultured cells, cell survival rate was analysis. Cell survival rate MTT (3 - [4,5 provided dimethylthiazol-a 2 a-yl] - 2,5 provided diohenyl tetrazolium bromide, Sigma chemical Co. USA) through his analyzing method. First, a 96 well L-a 929 cell line (1 × 105 Cell/well) 24 which is included in the time after the culturing, Control (MEM), Vehicle (Agar film comprises dipping medium), america compared to virtue L (paper mulberry 1g + 0. america compared to virtue 1g + 0. 5g film comprises dipping medium), america compared to virtue H (paper mulberry 1g + 0. america compared to virtue 1g + 1. 0g film comprises dipping medium) processing, him as period of time during the 24. Then, the culture solution after adding MTT solution number 50 and a stand-alone micro l, him as additional 4 hours. The cell a violet crystals are created by a process for the dimethyl (dimethly sulphoxide, DMSO, Sigma co.) solution into 150 micro l, process e cup ofhis anger. VERSA max microplate reader (Molecular Devices, Sunnyvale, CA, USA) color change appears in each well using measured at 540 nm and, 7 also have shown to result. As shown in fig. 7, a significant difference when compared to Control america compared to virtue shell powder which cannot be high mask pack sheet (H america compared to virtue, paper mulberry 1g + 0. america compared to virtue 1g + 1. 0g film comprises dipping medium) cell survival rate in the case of somewhat high 24.4, all prepared by the number mask pack sheet does not require such a her in combination with cytotoxic drugs. Although the present invention is mentioned as said preferred embodiment described but, on the various subject matter of invention deviates from the range without modification and deformation process from. In addition the modified belonging to the present subject matter of invention appended claims comprise a deformation. The present invention relates to a cosmetic composition comprising Styela clava skin and a natural polymer. More particularly, the present invention relates to a cosmetic composition which can prepare a functional beauty product having high value without stimulating the skin by comprising Styela clava skin and a natural polymer as active components. COPYRIGHT KIPO 2017 Active ingredient america compared to virtue shell and natural polymer including cosmetic composition. According to Claim 1, natural polymer obtained by grinding and then drying said shell paper mulberry characterized paper mulberry shell powder cosmetic composition comprising the same. Classification Agar 0. 25% Agar 0. 50% Agar 0. 75% Agar 1. 00% 0 time 147. 76 146. 62 147. 05 147. 63 1 time 147. 70 146. 55 146. 99 147. 56 2 time 147. 65 146. 50 146. 95 147. 53 4 time 147. 52 146. 38 146. 81 147. 44 8 time 147. 29 146. 17 146. 57 147. 20 16 time 146. 94 145. 74 146. 11 146. 66 24 time 146. 53 145. 33 145. 63 146. 21 The water vapor permeability (g/m2/hr) 30. 89 32. 48 35. 80 35. 50
