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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 7866. Отображено 100.
29-03-2012 дата публикации

Method of Producing Dried Distiller's Grain with Solubles Agglomerated Particles

Номер: US20120076915A1
Автор: Russel J. Meier
Принадлежит: Individual

A method of producing Dried Distiller's Grain with Solubles (DDGS) agglomerated particles is disclosed. The method comprises the steps of separating a first product stream out of the whole stillage that is primarily solid material from a second product stream that is primarily liquid, agglomerating the first product stream using a mechanical compression machine such as an extruder, and exposing the agglomerated particles to microwave radiation of a sufficient energy for a sufficient time so that the resulting moisture of the agglomerated particles is between 3% and 40% by weight. The second product stream may be condensed and blended with the first product stream prior to, or concurrently with, the step in which the agglomerated particles are formed. Additionally, other additives, such as enzymes, may be added to the first product stream to enhance the final product's desirability as a nutritional source.

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Номер записи: 1
10-05-2012 дата публикации

Polypeptides Having Phytase Activity and Polynucleotides Encoding Same

Номер: US20120114799A1
Автор: Carsten Sjoholm, Monica Takamiya Wik
Принадлежит: Novozymes AS

The invention relates to Citrobacter phytases derived from Citrobacter amalonaticus, Citrobacter gillenii , and related phytases. The phytases belong to the acid histidine phosphatase family, are acid-stable, and expectedly of a high specific activity. The invention also relates to the corresponding DNA, the recombinant and wild-type production of the phytases, as well as the use thereof, in particular in animal feed.

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Номер записи: 2
09-08-2012 дата публикации

Synthetic phytase variants

Номер: US20120201923A1
Автор: Beata Stosik, Serwe Annegret, Stefan Haefner
Принадлежит: BASF SE

The invention relates to a synthetic phytase having an increased thermal stability and also to an isolated nucleic acid sequence encoding a synthetic phytase, and the use of the phytase in an animal feed for reducing the phosphtate content in manure, and also animal feed additives and animal feeds comprising the synthetic phytase.

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Номер записи: 3
03-01-2013 дата публикации

Ts-23 alpha-amylase variants with altered properties

Номер: US20130005029A1
Автор: Brain E. Jones, Casper Vroemen, Chris Leeflang, Claudine Chang, Clement Choy, James T. Kellis, Jr., Luis G. Cascao-Pereira, Marc Kolkman, Melodie Estabrook, Walter Weyler
Принадлежит: DANISCO US INC

Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.

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Номер записи: 4
31-01-2013 дата публикации

Process For Preparing An Enzyme Containing Granule

Номер: US20130029010A1
Автор: Christian Pedersen, Erik Marcussen
Принадлежит: Novozymes AS

This invention relates to a process for manufacture of a dry enzyme containing mixer granulation granule comprising the step of adding a particulate component to the mixer granulation process, wherein the particulate component constitutes less than 75 parts of the finished granule and the particles of the particulate component have an mean size of more than 40 μm in its longest dimension. Also claimed is granules of the process, granules containing more than two particles of the particulate component and composition and methods of using the granules.

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Номер записи: 5
13-02-2014 дата публикации

Methods and compositions for improving growth of meat-type poultry

Номер: US20140044700A1
Автор: Jason C.H. Shih
Принадлежит: North Carolina State University

The present invention provides methods of improving growth performance, improving the efficiency of feed utilization, increasing feed digestibility, and decreasing mortality of immature and developing animals receiving animal feed. Methods of producing a crude keratinise enzyme extract and animal feed supplements for achieving the same are also provided.

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Номер записи: 6
20-03-2014 дата публикации

Optimizing egg production characteristics via seawater mineralization

Номер: US20140079803A1
Автор: Edward E. Anderson, Neal Rye, Timothy E. Luberski
Принадлежит: LUBERSKI Inc (DBA HIDDEN VILLA RANCH)

Aspects for utilizing a seawater fluid to optimize egg-production characteristics are disclosed. In one aspect, a method is provided, which includes identifying a desired optimization, determining a corresponding seawater to non-seawater ratio, and generating a seawater fluid according to the ratio. An egg-cultivation method is also provided, which includes ascertaining a desired optimization, selecting a corresponding seawater to non-seawater ratio, and providing a seawater fluid comprising the ratio to a hen. In another egg-cultivation method, hens are provided with a mixed feed comprising a feed to seawater-mineralized produce ratio selected according to a desired optimization. A method to grow produce is also provided, which includes mineralizing soil with a seawater fluid comprising a seawater to non-seawater ratio corresponding to a preferred optimization. In another aspect, a method to produce feed is disclosed, which includes mixing feed with mineralized produce according to a ratio corresponding to a desired optimization.

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Номер записи: 7
04-01-2018 дата публикации

SYSTEM AND METHOD FOR PREPARING MICRO-INGREDIENT FEED ADDITIVES TO ANIMAL FEED RATIONS

Номер: US20180000127A1
Автор: Freeman Steve, White Joshua A.
Принадлежит:

A method and system are provided for preparing micro-ingredient feed additives for use in designated feed rations. A micro-ingredient system of the invention includes a plurality of bins that store designated micro-ingredients therein. A master controller of the delivery system provides signals to control system components based on programmed commands corresponding to micro-ingredient batches to be prepared. Slide gate mechanisms are used to prevent loss of micro-ingredients delivered to a receiving receptacle of the system. During delivery of the micro-ingredients to the receptacle and during processing, the micro-ingredients can become airborne and subsequently lost. The slide gate mechanisms also provide controlled access to the receiving receptacle to prevent system errors such as contamination of ingredients in the receptacle, or improper batching of a prescribed micro-ingredient mixture to be delivered to a designated feed ration. 1. A method for preparing micro-ingredients to be used in designated feed rations , said method comprising: '(i) at least one bin for holding a micro-ingredient therein, said bin having an opening for receiving the micro-ingredient; (ii) a dispensing device communicating with the at least one bin for dispensing the micro-ingredient from the at least one bin; (iii) a receiving receptacle for receiving the micro-ingredient dispensed; (iv) a slide gate mechanism mounted to the receiving receptacle, the slide gate mechanism having a slidable gate; (v) a controller communicating with said dispensing device and said slide gate mechanism, said controller including a micro-processor, computer coded instructions determining operation of the system, and an interface for sending output control signals to and receiving input signals from said slide gate mechanism; and', '(a) providing(b) operating said slide gate mechanism based on commands from said computer coded instructions resulting in the output control signals sent to said slide gate ...

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Номер записи: 8
04-01-2018 дата публикации

Method for Mixing of Particles

Номер: US20180001280A1
Автор: Bach Poul
Принадлежит: NOVOZYMES A/S

Continuous mixing in a static mixer possible can be used to add one kind of particles (such as an enzyme granular product) in a small amount to a larger amount of a different kind of particles (such as a powder stream of detergent powder), even if the powder characteristics are substantially different, with substantially no damage to the enzyme particles and with a high degree of homogeneity. 1. A method for mixing of at least two kinds of particles , wherein a first and a second stream of particles are mixed in a static mixer , and wherein the first and the second stream of particles have a weight ratio below 1:20.2. The method of wherein the ratio is below 1:50 claim 1 , particularly in the range from 1:1000 to 1:100 claim 1 , or from 1:500 to 1:125.3. The method of claim 1 , wherein the average particle size of the first stream of particles is in the range 100-2000 μm.4. The method of claim 1 , wherein the average particle size of the first stream of particles is in the range 300-1200 μm.5. The method of claim 1 , wherein the coefficient of variation of the amount of particles from the first stream claim 1 , in the resulting mixture of the first and second stream of particles claim 1 , is less than 40%.6. The method of claim 5 , wherein the coefficient of variation is measured using at least 9 samples and a sample size of at least 5 g.7. The method of claim 1 , wherein the first stream comprises enzyme particles.8. The method of claim 7 , wherein the enzyme particles comprise a protease claim 7 , an amylase claim 7 , a carbohydrase claim 7 , a lipase claim 7 , a cellulase claim 7 , an oxidoreductase claim 7 , a mannanase or a pectate lyase or a phosphatase claim 7 , or a deoxyribonuclease.9. The method of claim 7 , wherein the resulting mixture of the enzyme particles and the second stream of particles has an amount of free enzyme dust below 20 parts per billion.10. The method of claim 1 , wherein the second stream comprises detergent granules or detergent powder ...

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Номер записи: 9
20-01-2022 дата публикации

Pasture Treatments for Enhanced Carbon Sequestration and Reduction in Livestock-Produced Greenhouse Gas Emissions

Номер: US20220015390A1
Автор: ALIBEK Ken, FARMER Sean, TSKHAY Albina
Принадлежит:

The subject invention provides compositions and methods for reducing deleterious atmospheric gases and/or precursors thereof using livestock feed additives and/or supplements. In preferred embodiments, a multi-purpose composition comprising one or more beneficial microorganisms and/or one or more microbial growth by-products is applied to livestock animals' feed and/or to a field or pasture where livestock animals graze. In some embodiments, the composition controls methanogenic bacteria. In some embodiments, the composition enhances carbon sequestration in the field or pasture by promoting plant health and/or growth. 1. A method for reducing enteric deleterious atmospheric gases and/or precursors thereof , the method comprising applying a composition comprising one or more beneficial microorganisms and/or one or more microbial growth by-products to a field or pasture , said field or pasture comprising grasses and/or other plants upon which livestock animals graze , and placing the livestock animals in the field or pasture to graze ,{'i': Bacillus amyloliquefaciens, Pleurotus ostreatus, Lentinula edodes, Trichoderma viridae, Wickerhamomyces anomalus, Saccharomyces cerevisiae, Saccharomyces boulardii, Starmerella bombicola, Meyerozyma guilliermondii, Pichia occidentalis, Monascus purpureus, Acremonium chrysogenum, Myxococcus xanthus, Bacillus subtilis', 'Bacillus lichenmformis., 'wherein the livestock animals ingest the composition in addition to the grasses and/or other plants, and wherein the one or more beneficial microorganisms are selected from and'}2. The method of claim 1 , wherein the livestock animals are ruminants.3Bacillus amyloliquefaciens.. The method of claim 1 , wherein at least one of the one or more beneficial microorganisms is a strain of4Bacillus amyloliquefaciensB. amyloliquefaciensB. amy. The method of claim 3 , wherein the strain of is NRRL B-67928 (“”).5B. amy, P. ostreatusS. boulardii.. The method of claim 1 , wherein the one or more ...

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Номер записи: 10
12-01-2017 дата публикации

Use of Bacterial Amylases in Feed for Poultry

Номер: US20170006895A1
Автор: HERMES Rafael Gustavo, KNAP Inge, SORBARA Jose-Octavio
Принадлежит:

The present invention relates to methods and uses of at least one bacterial amylase in poultry feed to improve the nutritional value of the feed. The invention also relates to poultry feed and poultry feed additives comprising at least one bacterial amylase and at least one vitamin and/or mineral which improve the nutritional value of the feed. 115-. (canceled)16. A method for improving the nutritional value of poultry feed , comprising adding at least one amylase to the feed , wherein the amylase is a polypeptide having at least 80% identity to amino acids 1-481 of SEQ ID NO: 2.17. The method of claim 16 , wherein the amylase has at least 85% identity to amino acids 1-481 of SEQ ID NO: 2.18. The method of claim 16 , wherein the amylase has at least 90% identity to amino acids 1-481 of SEQ ID NO: 2.19. The method of claim 16 , wherein the amylase has at least 95% identity to amino acids 1-481 of SEQ ID NO: 2.20. The method of claim 16 , wherein the amylase has at least 97% identity to amino acids 1-481 of SEQ ID NO: 2.21. The method of claim 16 , wherein the amylase comprises amino acids 1-481 of SEQ ID NO: 2.22. The method of claim 16 , wherein the poultry feed has reduced apparent metabolisable energy compared to a standard poultry feed.23. The method of claim 16 , wherein the amylase is added to the poultry feed at a dose of between 0.01 and 200 mg enzyme protein per kg diet.24. The method of claim 16 , for improving weight gain and/or feed conversion ratio of poultry.25. A poultry feed additive comprising at least one amylase claim 16 , together with (i) at least one vitamin claim 16 , (ii) at least one mineral claim 16 , or (iii) at least one vitamin and at least one mineral claim 16 , wherein the bacterial amylase is a polypeptide having at least 80% identity to amino acids 1-481 of SEQ ID NO: 2.26. The poultry feed additive of claim 25 , wherein the poultry feed additive improves the nutritional value of a poultry feed.27. The poultry feed additive of claim ...

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Номер записи: 11
12-01-2017 дата публикации

A Method for Improving Maize Digestibility in Bovine Animals

Номер: US20170006896A1
Автор: Schlifka Wolfgang, Schwemer Antje, Stenssons Anna
Принадлежит:

The invention relates to the use of at least one bacterial amylase in combination with one or more protease(s) in feed for ruminant animals of the subfamily Bovinae for improving digestibility of maize and/or maize silages, in particular for improving milk yield, weight gain, and/or Feed Conversion Ratio (FCR). Examples of bovine animals are dairy cows and beef cattle. The invention also relates to the use of such enzyme combinations in feed additive compositions and animal feed compositions enriched with maize and maize silages. 111-. (canceled)12. A method for improving the digestibility of maize and/or maize silages in feed for animals of the subfamily Bovinae , comprising treating a maize source with an effective amount of one or more proteolytic enzymes in combination with at least one amylase.13. A method for increasing the milk yield , increasing the weight gain or improving the feed conversion ratio of an animal of the subfamily Bovinae , comprising adding at least one bacterial amylase and one or more proteolytic enzymes to the feed which comprises maize and/or maize silages.14. A feed composition comprising at least one bacterial amylase , one or more proteolytic enzymes , and maize and/or maize silage. High-yielding cows in modern farming systems live under conditions that are characterised by a very high milk production (dairy cows) or growth rate (beef cattle), which is followed by an equally high energy requirement. The utilisation of the feed decreases markedly when intake is increased beyond maintenance levels. In part to account for this, more and more easily degradable feed is included into the ruminant feed e.g. starch-containing raw materials such a cereal-based concentrates and whole cereal silages. The starchy material is frequently recovered in the faeces implying that the utilisation of such feed ingredients could be enhanced further.Maize or maize silage is becoming more common in increasing amounts in feed rations, in particular in feed for ...

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Номер записи: 12
14-01-2021 дата публикации

Veterinary Composition Comprising Superoxide Dismutase and at Least One Hydrolysate of Proteins Rich in Bioassimilable Peptides

Номер: US20210008174A1
Автор: Destaing Cécile, Renaglia Pascal
Принадлежит:

A veterinary composition comprising, in effective amounts, superoxide dismutase and at least one protein hydrolysate rich in bioassimilable peptides. 1. A veterinary composition , which can preferably be administered orally , comprising , in effective amounts:superoxide dismutase or at least one source of superoxide dismutase, andat least one protein hydrolysate or at least one preparation based on at least one protein hydrolysate, said at least one protein hydrolysate advantageously being devoid of proteins, said at least one protein hydrolysate comprising a peptide fraction in which less than 1% by weight of the peptides have a molecular weight greater than or equal to 10,000 Da, preferably in which 100% by weight of the peptides have a molecular weight less than 10,000 Da, preferably less than 3,000 Da, advantageously less than or equal to 1,800 Da, in a preferred manner less than 1,800 Da.2. The composition according to claim 1 , wherein said peptide fraction represents at least 50% by weight claim 1 , preferably more than 50% by weight claim 1 , preferably at least 55% by weight claim 1 , advantageously at least 60% by weight claim 1 , in a preferred manner more than 60% by weight claim 1 , relative to the total weight of said at least one protein hydrolysate.3. The composition according to or comprising at least one source of superoxide dismutase and at least one preparation based on at least one protein hydrolysate in a weight ratio between 0.01:100 and 100:1 claim 1 , preferably between 1:100 and 1:10 claim 1 , preferably between 1:100 and 5:100 claim 1 , advantageously between 1:50 and 2:50.4. The composition according to any one of the preceding claims claim 1 , wherein said superoxide dismutase is of plant origin claim 1 , preferably of fruit origin claim 1 , advantageously derived from Olea europeae claim 1 , from Vitis vinifera and/or from at least one Cucurbitaceae such as Cucumis melo; in a preferred manner said superoxide dismutase consisting of the ...

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Номер записи: 13
12-01-2017 дата публикации

PROTEIN

Номер: US20170009220A1
Автор: Arent Lund Susan, Barends Sharief, Haaning Svend, Koops Bert, Lorentsen Rikke Hoeegh, Nikolaev Igor, Van Tuijl Jan Hendrick A
Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

The present invention relates to an isolated polypeptide having xylanase activity, selected from the group consisting of: 1. An isolated polypeptide having xylanase activity , selected from the group consisting of:(a) a polypeptide comprising an amino acid sequence having at least 87% identity with SEQ ID NO: 1;(b) a polypeptide encoded by a polynucleotide having at least 87% identity with SEQ ID NO:2; or(c) a fragment of a polypeptide of a) or b) which fragment has xylanase activity.2. The polypeptide according to claim 1 , wherein the polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID No. 1 claim 1 , SEQ ID No: 3 claim 1 , SEQ ID No. 5 claim 1 , SEQ ID No. 7 claim 1 , SEQ ID No. 9 and SEQ ID No. 11.3. The polypeptide according to or claim 1 , wherein the polypeptide consists of an amino acid sequence selected from the group consisting of SEQ ID No. 1 claim 1 , SEQ ID No: 3 claim 1 , SEQ ID No. 5 claim 1 , SEQ ID No. 7 claim 1 , SEQ ID No. 9 and SEQ ID No. 11.4. The polypeptide according to any one of to wherein the polypeptide is encoded by a polynucleotide selected from the group consisting of: SEQ ID No. 2 claim 1 , SEQ ID No. 4 claim 1 , SEQ ID No. 6 claim 1 , SEQ ID No. 8 claim 1 , SEQ ID No. 10 and SEQ ID No. 12.5. The polypeptide of any of - claim 1 , wherein the polypeptide is an endo-1 claim 1 ,4-β-xylanase.6. The polypeptide of any of one of the preceding claims claim 1 , wherein the polypeptide has at least 50% residual activity of xylanase activity after incubation at 65° C. for 10 minutes at pH 6 and/or has at least 80% residual activity of xylanase activity after incubation at 61° C. for 10 minutes at pH 6.7. The polypeptide of any of the preceding claims claim 1 , wherein the polypeptide has a residual activity of at least 70% when incubated with 0.2 mg/ml pepsin in a buffered solution at pH3.5 for two hours at a temperature of 40° C.8. The polypeptide of any of the preceding claims claim 1 , wherein the ...

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Номер записи: 14
03-02-2022 дата публикации

Feed Additive, Method of Preparation, and Use in Order to Improve the Zootechnical Performance of a Ruminant

Номер: US20220030917A1
Автор: Bravo David, Wall Emma
Принадлежит:

The invention relates to a feed additive comprising at least one nutrient, which non-nutrient is incorporated in a matrix, the entirety being encapsulated by a coating comprising at least a plant material whose saponification number is greater than 180. 16-. (canceled)7. Use of a feed additive comprising at least one non-nutrient , which non-nutrient is incorporated in a matrix , the entirety being encapsulated by a coating comprising at least a plant material whose saponification number is greater than 180 , to improve the zootechnical performance of a ruminant.8. Use according to claim 7 , in which the zootechnical performance is at least one of the following performances: milk production claim 7 , milk quality claim 7 , altered milk composition claim 7 , altered feed consumption claim 7 , altered eating behavior claim 7 , improved average weight gain claim 7 , increased food efficiency claim 7 , improved insulin sensitivity of the digestive system claim 7 , improved digestive health claim 7 , and improved immunity.9. A method for preparing a feed additive comprising the following steps:a) at least one non-nutrient, in the form of particles, is chosen;b) at least one compound forming a matrix is optionally chosen for the non-nutrient(s), and the compound(s) is(are) incorporated into the matrix in a manner so as to obtain the particles;c) it is assessed whether the non-nutrient is broken down in the rumen when it is left unprotected;d) if the non-nutrient is broken down beyond an initial threshold, go to point f);e) if the non-nutrient is not broken down beyond an initial threshold, it is adopted, with its optional matrix, and we go to point l);f) at least one compound forming a matrix is optionally chosen for the non-nutrient(s), and the non-nutrient(s) is(are) incorporated into the matrix in a manner so as to obtain the particles;g) at least one coating compound is chosen;h) the non-nutrient(s) and the optional compounds forming a matrix is(are) coated through ...

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Номер записи: 15
03-02-2022 дата публикации

ANIMAL FEED COMPOSITION AND USE THEREOF

Номер: US20220031815A1
Автор: AMERI Javier Alejandro, CARDOSO BITTENCOURT Leticia, HIDALGO Marcelo, LOPEZ-ULIBARRI Rual, MAKHIJA Vijay, PEREZ CALVO Estefania, VALIENTES Rolando
Принадлежит:

The present invention provides a composition and/or an animal feed comprising polypeptides having muramidase activity and uses thereof. 1. A composition comprising one or more polypeptides having muramidase activity and one or more antibiotics.2. The composition of claim 1 , wherein the polypeptide having muramidase activity is a fungal GH24 muramidase or GH25 muramidase.3. The composition of claim 1 , wherein the antibiotic is any one of Bacitracin claim 1 , Bambermycin claim 1 , Carbadox claim 1 , Enramycin claim 1 , Enduracidin claim 1 , Laidlomycin claim 1 , Lasalocid claim 1 , Lincomycin claim 1 , Monensin claim 1 , Neomycin claim 1 , Penicillin claim 1 , Roxarsone claim 1 , Roxarsone claim 1 , Salinomycin claim 1 , Tylosin claim 1 , and Virginiamycin.4. The composition of claim 1 , wherein the polypeptide having muramidase activity is selected from the group consisting of:(a) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 1;(b) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 2;(c) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 3;(d) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 4;(e) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 5;(f) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 6;(g) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 7;(h) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 8;(i) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at ...

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Номер записи: 16
16-01-2020 дата публикации

Trypsin-like serine proteases and uses thereof

Номер: US20200015499A1
Автор: Shukun Yu, Stephen Shipovskov, Xiaogang Gu, Zhengzheng ZOU
Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

Novel trypsin-like serine proteases and uses thereof are described herein.

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Номер записи: 17
16-01-2020 дата публикации

BIOACTIVE COMPOUNDS PROTECTION METHOD AND COMPOSITIONS CONTAINING THE SAME

Номер: US20200016083A1
Автор: BARZILAY Amir, Koren-Lichtig Hagit
Принадлежит:

The present invention relates to means for protecting bioactive materials in mammalian food or feed formulations used to enhance the health status of mammals. 1. A method for encapsulating or embedding a heat sensitive bioactive ingredient in mammalian newborn food formulation or mammalian newborn feed formulation , comprising the steps of;(i) mixing a heat sensitive bioactive ingredient with an encapsulating material, wherein said encapsulating material is food-grade, feed-grade materials, either alone or in combination, with a liquid, forming a liquid blend,(ii) drying the liquid blend forming a dry blend,(iii) coating the dry blend with at least one additional encapsulating layer;(iv) mixing the dry blend with at least one additional food-grade or feed-grade material; and(v) adding the dry blend to the mammalian newborn formulation, wherein the steps of admixing all of the ingredients and drying are conducted at a temperature below 50° C., such that the activity of the heat sensitive bioactive ingredient is substantially maintained, thereby encapsulating or embedding the heat sensitive bioactive ingredient in a mammalian newborn formulation.2. The method of claim 1 , wherein the mammalian newborn food or feed is a human infant formula.3. The method of claim 1 , wherein the mammalian newborn food or feed is a milk replacer claim 1 , a milk substitute or a combination thereof.4. The method of claim 1 , wherein the step of drying the liquid blend further comprises a step of grinding the dry blend.5. The method of claim 1 , wherein the heat sensitive bioactive ingredient is selected from the group consisting of insulin claim 1 , a glycoprotein claim 1 , an immunoglobulin claim 1 , a peptide claim 1 , a polypeptide claim 1 , a hormone claim 1 , an enzyme claim 1 , IGF-I claim 1 , IGF-II claim 1 , EGF alpha-1 proteinase inhibitor claim 1 , alkaline phosphatase claim 1 , angiogenin claim 1 , antithrombin III claim 1 , chitinase claim 1 , extracellular superoxide ...

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Номер записи: 18
17-01-2019 дата публикации

BACTERIA AND ENZYMES PRODUCED THEREFROM AND METHODS OF USING SAME

Номер: US20190016997A1
Автор: Ayangbile Gbenga, Grzemski Mary, JR. James F., Krueger Lucas, Spangler David, Tobey
Принадлежит:

A bacteria referred to here as 6A-1 is provided, compositions thereof and processes for use of the bacteria, spores, cells, extracts and enzymes. The compositions which comprise the bacteria, spores, cells, extracts and/or enzymes are capable of degrading polysaccharides. Such compositions are capable of degrading cellulose, including plant-produced cellulose, microcrystalline cellulose and carboxymethyl cellulose. The bacteria produces at least two cellulose-degrading protein fractions. Cellulose degrading activity continues across pH2 to pH13. 125.-. (canceled)26Bacillus subtilis. A method of degrading a composition comprising at least one polysaccharide , said method comprising combining a composition comprising 6A-1 (6A-1) , reference culture comprising said 6A-1 having been deposited at ATCC under deposit number PTA-125135 , or cells of said 6A-1 , or spores produced by said 6A-1 , or at least one polysaccharide-degrading enzyme extracted from said 6A-1 , or combination thereof , wherein said 6A-1 or cells or spores are filtered , dried , freeze dried or ground , or have added thereto at least one excipient , carrier or diluent to , and contacting said composition comprising said 6A-1 or cells or spores or at least one polysaccharide-degrading enzyme or combination thereof with said composition comprising said at least one polysaccharide and degrading said at least one polysaccharide.27. The method of claim 26 , wherein said composition comprising said 6A-1 or cells or spores or at least one polysaccharide-degrading enzyme or combination thereof comprises at least three cellulose-degrading protein fractions produced by said 6A-1 claim 26 , said protein fractions capable of degrading crystalline cellulose claim 26 , carboxymethyl cellulose and unmodified cellulose.28. The method of claim 26 , wherein composition comprising said 6A-1 or cells or spores or at least one polysaccharide-degrading enzyme or combination thereof is capable of degrading protein at a pH ...

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Номер записи: 19
25-01-2018 дата публикации

A METHOD FOR IMPROVING FEED DIGESTIBILITY AND GROWTH PERFORMANCE

Номер: US20180021298A1
Автор: COWIESON Aaron, KNAP Inge
Принадлежит:

The invention provides a method for improving growth performance animals. In particularly, the inventors of the invention found that the addition of a proteolytic enzyme (protease) to regular animal diets results in a significant improvement of growth performance if the proteolytic enzyme is supplemented and combined with vitamin C. 1. A method for improving growth performance of farm animals , the method comprising the step of treating a regular animal diet with an efficient amount of one or more proteolytic enzyme (protease) in combination with vitamin C , a derivative or metabolite thereof.2. The method of claim 1 , wherein the growth performance is measured as average daily weight gain (ADWG) claim 1 , average daily feed intake (ADFI) and/or feed conversion rate (FCR) of the animal.3Nocardiopsis dassonvilleidassonvilleiNocardiopsis prasinaNocardiopsis prasinaNocardiopsisNocardiopsis alkaliphilaNocardiopsis lucentensis. The method according to claim 1 , wherein the protease is an acid stable serine proteases derived from subsp. DSM 43235 (A1918L1) claim 1 , DSM 15649 (NN018335L1) claim 1 , (previously alba) DSM 14010 (NN18140L1) claim 1 , sp. DSM 16424 (NN018704L2) claim 1 , DSM 44657 (NN019340L2) and DSM 44048 (NN019002L2) claim 1 , as well as homologous proteases.4. The method of any of claim 1 , wherein the animal is selected from the group consisting of non-ruminants and ruminants claim 1 , ruminants include but not limited to sheep claim 1 , goat and cattle claim 1 , and non-ruminants include but not limited to horse; rabbit; pig including but not limited to infant pig claim 1 , piglet claim 1 , growing-fattening pig claim 1 , sow and boar; and poultry such as turkey claim 1 , duck and chicken.5. The method of claim 1 , wherein the intended dosage of the protease is 0.01-200 mg protease enzyme protein per kg diet.6. The method of any of claim 1 , wherein the intended dosage of vitamin C is 50-500 mg vitamin C per kg diet.7. Use of one or more proteolytic ...

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Номер записи: 20
25-01-2018 дата публикации

A METHOD FOR IMPROVING FEED DIGESTIBILITY IN BOVINE ANIMALS

Номер: US20180021415A1
Автор: ACEDO Tiago, IMMIG Irmgard, TAMASSIA Luis
Принадлежит:

The invention relates to the use of at least one bacterial amylase in combination with a mixture of at least two essential oil compounds selected from the group consisting of thymol, eugenol, meta-cresol, vaniline and guajacol in feed for ruminant animals of the subfamily Bovinae for improving weight gain, milk yield and/or Feed Conversion Ratio (FCR). Examples of animals are beef cattle and dairy cows. 1. A method of improving weight gain , milk yield and/or feed conversion ratio of animals of the subfamily Bovinae , characterized by administering to the animal at least one carbohydrase in combination with a mixture of at least two , preferably at least three essential oil compounds selected from the group consisting of thymol , eugenol , meta-cresol , vaniline and guajacol.2. The method according to claim 1 , wherein the essential oils are administered in amounts (total dosage ranges of essential oils) of 50 to 150 mg per kg body weight per day claim 1 , preferably 70 mg to 120 mg per kg body weight per day.3. The method according to claim 1 , wherein the animal is a domestic cattle claim 1 , in particular a beef cattle.4. The method according to claim 1 , wherein the animal is a dairy cow.5. The method according to claim 1 , wherein the at least one carbohydrase is an amylase.6. Use of at least one carbohydrase in combination with a mixture of at least two claim 1 , preferably at least three essential oil compounds selected from the group consisting of thymol claim 1 , eugenol claim 1 , meta-cresol claim 1 , vaniline and guajacol in feed for animals of the subfamily Bovinae for improving weight gain claim 1 , milk yield and or feed conversion ratio.7. Use according to claim 6 , wherein the essential oils are administered in amounts (total dosage ranges of essential oils) of 50 to 150 mg per kg body weight per day claim 6 , preferably 70 mg to 120 mg per kg body weight per day.8. Use according to claim 6 , wherein the animal is a domestic cattle claim 6 , in ...

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Номер записи: 21
10-02-2022 дата публикации

FEED ADDITIVE COMPOSITION

Номер: US20220040241A1
Автор: Bernardeau Marion, Davis Mari Ellen, Forssten Sofia, Galbraith Elizabeth Ann, ISAKSEN MAI FAURSCHOU, KIARIE ELIJAH G., LUND SUSAN ARENT, MILLAN LUIS FERNANDO ROMERO, NURMINEN PÄIVI, Petri Daniel
Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-β-d-xylanase) and a β-glucanase (and optionally a further fibre degrading enzyme). 1. A method for improving the performance of a subject or for improving digestibility of a raw material in a feed , or for improving nitrogen retention , or for improving feed conversion ratio (FCR) , or for improving weight gain in a subject , or for improving feed efficiency in a subject , or for shifting the fermentation process in the subject's gastrointestinal tract towards the production of butyric acid and/or volatile fatty acids (VFA) , the method comprising{'i': 'Bacillus', 'administering a direct fed microbial (DFM) comprising strains AGTP BS3BP5 (NRRL B-50510), AGTP BS918 (NRRL B-50508), and AGTP BS1013 (NRRL B-50509), to the subject, in combination with a xylanase and a β-glucanase.'}2. The method of claim 1 , wherein the DFM is a viable bacterium.3. The method of claim 1 , wherein the direct fed microbial is in the form of an endospore.4. The method of claim 1 , wherein the xylanase is an endo-1 claim 1 ,4-β-d-xylanase.5. The method of claim 1 , further comprising administration of a further fibre degrading enzyme.6. The method of claim 5 , wherein the further fibre degrading enzyme is selected from the group consisting of a cellobiohydrolase (E.C. 3.2.1.176 and E.C. 3.2.1.91) claim 5 , a β-glucosidase (E.C. 3.2.1.21) claim 5 , a β-xylosidase (E.C. 3.2.1.37) claim 5 , a feruloyl esterase (E.C. 3.1.1.73) claim 5 , an α-arabinofuranosidase (E.C. 3.2.1.55) claim 5 , a pectinase (E.C. 3.2.1.15) claim 5 , an exopolygalacturonase (E.C. 3.2.1.67) claim 5 , a pectate lyase (E.C. 4.2.2.2)) claim 5 , and combinations thereof.7. The method of claim 5 , wherein the further fibre degrading enzyme is selected from the group consisting of a cellobiohydrolase (E.C. 3.2.1.176 and E.C. 3.2.1.91) claim 5 , a β-glucosidase (E.C. 3.2.1.21) claim 5 , and combinations thereof.8. ...

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Номер записи: 22
10-02-2022 дата публикации

ANIMAL FEED COMPOSITIONS AND USES THEREOF

Номер: US20220040271A1
Автор: LOPEZ-ULIBARRI Rual, PEREZ CALVO Estefania
Принадлежит:

The present invention relates to animal feed compositions comprising polypeptides having muramidase activity and carotenoids and uses thereof. 1. A composition comprising one or more polypeptides having muramidase activity and one or more carotenoids.2. The composition of claim 1 , wherein the polypeptide having muramidase activity is a fungal GH24 muramidase or GH25 muramidase.3. The composition of claim 1 , wherein the carotenoid is selected from the group consisting of lutein claim 1 , canthaxanthin claim 1 , zeaxanthin claim 1 , astaxanthin and derivatives thereof.4. The composition of claim 1 , wherein the polypeptide having muramidase activity is selected from the group consisting of:(a) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 1;(b) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 2;(c) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 3;(d) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 4;(e) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 5;(f) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 6;(g) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 7;(h) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 8;(i) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 9;(j) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 10;(k) a ...

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Номер записи: 23
22-01-2015 дата публикации

Non-digestible sugar-coated products and process

Номер: US20150024057A1
Автор: Guy W. Miller
Принадлежит: Guy W. Miller

A method and composition are provided for coating a component to achieve colon-targeted delivery. A component is coated with a fructose-based non-digestible carbohydrate such as a inulin, fructo-oligosaccharide or neosugar. The coated component is orally administered to a monogastric animal. The non-digestible coating causes the composition to pass through the stomach and small intestine without being degraded, and delivers the component to the colon where the coating is digested by microbial fermentation and the component is released.

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Номер записи: 24
22-01-2015 дата публикации

Enzyme Granules

Номер: US20150024081A1
Автор: Erik Kjaer Markussen, Erik Marcussen, Flemming Borup, Ole Simonsen
Принадлежит: Novozymes AS

The present application relates to a steam treated pelletized feed composition comprising a granule comprising a core and a coating wherein the core comprises an active compound and the coating comprises a salt.

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Номер записи: 25
26-01-2017 дата публикации

A method for producing a phytase variant with improved thermal stability, and a phytase variant and the use thereof

Номер: US20170022486A1
Автор: BAI Yingguo, HUANG Huoqing, LUO Huiying, Ma Rui, Meng Kun, SHAO Chao, SHI PENGJUN, WANG Yaru, YANG PEILONG, Yao Bin, Zhao Heng
Принадлежит:

The present invention relates to the field of genetic engineering, in particular, the present invention relates to a method for producing a phytase variant with an improved thermal stability, and a phytase variant and the use thereof. The phytase variant contains at least one proline modification, compared to the phytase from and other mutants thereof. The phytase variants with the modification have preferably improved properties, such as the thermal stability, optimal reaction temperature, pH property, specific activity, protease resistance and performance in animal feeds. 1. A method of preparing phytase variants having an improved thermostability , includes the step of introducing one or more prolines at the sites of S80 , S151 , T161 , N176 , S187 and/or A380P of the phytase with the amino acid sequence set forth in SEQ ID NO:2 , wherein said phytase variants is at least 70% homologous to the amino acid sequence set forth in SEQ ID NO:2.2. The method according to claim 1 , is characterized in that six prolines are introduced at the sites of S80 claim 1 , S151 claim 1 , T161 claim 1 , N176 claim 1 , S187 and A380P of the phytase with the amino acid sequence set forth in SEQ ID NO:2.3. Phytase variants having an improved thermostability with the following characteristics:Having at least 70% homology to the amino acid sequence set forth in SEQ ID NO:2; andone or more prolines are introduced at the sites of S80, S151, T161, N176, S187 and/or A380P of the amino acid sequence set forth in SEQ ID NO:2.4. The phytase variants according to claim 3 , is characterized in that six prolines are introduced at the sites of S80 claim 3 , S151 claim 3 , T161 claim 3 , N176 claim 3 , S187 and A380P of the phytase with the amino acid sequence set forth in SEQ ID NO:2.5. A polypeptide encoding the phytase variants having an improved thermostability of6. A nucleotide constructor comprising the polypeptide of claim 5 , and a regulating sequence optionally connected to one or more ...

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Номер записи: 26
24-01-2019 дата публикации

GLYCOSIDE HYDOLASES AND THEIR USE IN PREVENTING AND/OR TREATING A PATHOGENIC INFECTION IN AN ANIMAL

Номер: US20190022193A1
Автор: Haaning Svend, Poulsen Charlotte Horsmans
Принадлежит:

Disclosed are methods and compositions using glycoside hydrolases, such as an alpha-L-fucosidases, to prevent and/or treat a pathogenic infection and/or diarrhea in an animal wherein the pathogenic infection is caused by a pathogen capable of binding to an animal intestinal cell wherein said binding of the pathogen is dependent on the presence of a pathogen binding site having at least one glycan structure substituted with at least one alpha-1,2-L-fucose moiety comprising administering to the animal an effective amount of a glycoside hydrolase capable of removing the at least one alpha-1,2-L-fucose moiety from the pathogen binding site. 1. A method of preventing and/or treating an animal from having an intestinal pathogenic infection and/or diarrhea wherein the pathogenic infection and/or diarrhea is caused by a pathogen capable of binding to an animal intestinal cell wherein said binding of the pathogen is dependent on the presence of a pathogen binding site having at least one glycan structure substituted with at least one alpha-1 ,2-L-fucose moiety comprising administering to the animal an effective amount of a glycoside hydrolase capable of removing the at least one alpha-1 ,2-L-fucose moiety from the pathogen binding site.2. The method of wherein the glycoside hydrolase is an alpha-L-fucososidase.3. 3. The method of wherein the alpha-L-fucosidase is selected from the group consisting of glycoside hydrolase family 95 (GH95) and glycoside hydrolase family 29 (GH 29).4. The method of wherein the alpha-L-fucosidase is capable of removing a terminal alpha-1 claim 2 ,2-linked fucose group from a glycan-containing structure either alone or in combination with an enzyme capable of (a) converting a blood group A antigen to a blood group H antigen or (b) converting a blood group B antigen to blood group H antigen.5Escherichia coli. The method of wherein the pathogen is expressing F18 fimbriae.6. The method of or wherein the method further comprises administering to the ...

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Номер записи: 27
25-01-2018 дата публикации

G24 GLUCOAMYLASE COMPOSITIONS AND METHODS

Номер: US20180023066A1
Автор: Banerjee Goutami, Oo Khin, Yang Jie, Zhang Xiyun, ZHANG Yingxin
Принадлежит:

The invention is directed to variant glucoamylases as compared to SEQ ID NO:1, including amino acid substitutions at positions including 24, 32, 47, 71, 75, 88, 102, 104, 114, 118, 190, 201, 204, 260, 228, 230, 269, 271, 272, 281, 283, 284, 285, 293, 350, 313, 321, 338, 343, 367, 371, 386, 372, 401, 408, 420, 448, 463, 483, 484, 507, 530, 563 599 and 605 that retain glucoamylase activity. 1. A composition comprising a variant glucoamylase enzyme comprising an amino acid substitution as compared to SEQ ID NO:1 , wherein said amino acid substitution is at a position selected from the group of: 114 , 530 , 190 , 599 , 24 , 32 , 47 , 71 , 75 , 88 , 102 , 104 , 118 , 201 , 204 , 260 , 228 , 230 , 269 , 271 , 272 , 281 , 283 , 284 , 285 , 293 , 350 , 313 , 321 , 338 , 343 , 367 , 371 , 386 , 372 , 401 , 408 , 420 , 448 , 463 , 483 , 484 , 507 , 563 and 605 , wherein said variant enzyme is at least 95% identical to SEQ ID NO:1 , and wherein the variant enzyme retains glucoamylase activity.2. A composition comprising a variant glucoamylase enzyme comprising an amino acid substitution as compared to SEQ ID NO:1 , wherein said amino acid substitution is at a position selected from the group of: 114 , 530 , 190 , 599 , 24 , 32 , 47 , 71 , 75 , 88 , 102 , 104 , 118 , 201 , 204 , 260 , 228 , 230 , 269 , 271 , 272 , 281 , 283 , 284 , 285 , 293 , 350 , 313 , 321 , 338 , 343 , 367 , 371 , 386 , 372 , 401 , 408 , 420 , 448 , 463 , 483 , 484 , 507 , 563 and 605 , wherein said variant glucoamylase enzyme has at least 1.1 fold increased activity as compared to SEQ ID NO:1 under a condition selected from the group consisting of thermostability at 40° C. , thermostability at 52° C. , thermostability at 56° C. and thermostability at 60° C. , and wherein said variant enzyme is at least 95% identical to SEQ ID NO:1.3. A composition according to claim 1 , wherein said amino acid substitution is selected from the group consisting of: A114G claim 1 , A530T claim 1 , I190V claim 1 , N599D claim ...

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Номер записи: 28
23-01-2020 дата публикации

PROCESSING BIOMASS

Номер: US20200024622A1
Автор: Medoff Marshall
Принадлежит:

Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids. 1. A method of producing a polyester polymer comprising:(i) saccharifying biomass to form a saccharified biomass;(ii) contacting the saccharified biomass with a microorganism to produce one or more products; and(iii) converting the one or more products to the polyester polymer.2. The method of claim 1 , wherein the polyester polymer comprises poly(lactic acid).3. The method of claim 1 , wherein the one or more products comprises an organic acid.4. The method of claim 3 , wherein the organic acid comprises lactic acid.5. The method of claim 1 , wherein the microorganism comprises a lactic acid bacteria (LAB).6Lactobacillus.. The method of claim 1 , wherein the microorganism comprises a species in the genera7. The method of claim 1 , further comprising treating the biomass prior to step (i) to reduce its recalcitrance.8. The method of claim 7 , wherein the treating comprises size reduction (e.g. claim 7 , mechanical size reduction of individual pieces of biomass) claim 7 , radiation claim 7 , sonication claim 7 , pyrolysis claim 7 , or oxidation9. The method of claim 7 , wherein the treating comprises radiation claim 7 , e.g. claim 7 , using ionizing radiation.10. The method of claim 7 , wherein the recalcitrance of the biomass is reduced by at least about 10%.11. The method of claim 7 , wherein the recalcitrance of the biomass is reduced by 50%-90%.12. The method of claim 1 , wherein the biomass is a lignocellulosic or cellulosic material.13. The method of claim 1 , wherein the biomass comprises paper claim 1 , paper products claim 1 , paper waste claim 1 , wood claim 1 , particle board claim 1 , sawdust claim 1 , agricultural waste claim 1 , sewage claim 1 , silage claim 1 , grasses claim 1 , rice hulls claim 1 , bagasse claim 1 , cotton claim 1 , jute claim 1 , hemp claim 1 , flax claim 1 , bamboo ...

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Номер записи: 29
29-01-2015 дата публикации

Glycosylation as a stabilizer for phytase

Номер: US20150030717A1
Автор: Mariliz ORTIZ-MALDONADO, Mark S. Gebert, Michael Ward, Sang-Kyu Lee
Принадлежит: DANISCO US INC

The present teachings provide modified enzymes, preferably phytases, which have increased stability, hypothesized to arise from increased glycosylation. The enzymes can be modified to introduce or increase the number of glycosylation sites in the amino acid sequence, or glycosylation can be increased by the use of specific host production methods, or both. The enzymes of the present teachings have an increased stability after treatment at elevated temperature, which can be measured by inactivity reversibility or percent recovery following a treatment such as heating. The enzymes of the present teachings find application for example in food, feed, and feed pelleting.

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Номер записи: 30
17-02-2022 дата публикации

ANIMAL FEED COMPOSITION AND USE THEREOF

Номер: US20220046954A1
Автор: AURELI Raffaella, CARDOSO BITTENCOURT Leticia, LOPEZ-ULIBARRI Rual, PEREZ CALVO Estefania
Принадлежит:

The present invention relates to a method of improving flock uniformity and/or meat quality of a monogastric animal comprising administering to the animal a composition, an animal feed or an animal feed additive comprising one or more microbial muramidases. 1. A method of improving flock uniformity and/or meat quality of a monogastric animal comprising administering to the animal a composition , an animal feed or an animal feed additive comprising one or more microbial muramidases.2. The method of claim 1 , wherein the monogastric animal is selected from the group consisting of swine claim 1 , piglet claim 1 , growing pig claim 1 , sow claim 1 , poultry claim 1 , turkey claim 1 , duck claim 1 , quail claim 1 , guinea fowl claim 1 , goose claim 1 , pigeon claim 1 , squab claim 1 , chicken claim 1 , broiler claim 1 , layer claim 1 , pullet and chick claim 1 , cat claim 1 , dog claim 1 , horse claim 1 , crustaceans claim 1 , shrimps claim 1 , prawns claim 1 , fish claim 1 , amberjack claim 1 , arapaima claim 1 , barb claim 1 , bass claim 1 , bluefish claim 1 , bocachico claim 1 , bream claim 1 , bullhead claim 1 , cachama claim 1 , carp claim 1 , catfish claim 1 , catla claim 1 , chanos claim 1 , char claim 1 , cichlid claim 1 , cobia claim 1 , cod claim 1 , crappie claim 1 , dorada claim 1 , drum claim 1 , eel claim 1 , goby claim 1 , goldfish claim 1 , gourami claim 1 , grouper claim 1 , guapote claim 1 , halibut claim 1 , java claim 1 , labeo claim 1 , lai claim 1 , loach claim 1 , mackerel claim 1 , milkfish claim 1 , mojarra claim 1 , mudfish claim 1 , mullet claim 1 , paco claim 1 , pearlspot claim 1 , pejerrey claim 1 , perch claim 1 , pike claim 1 , pompano claim 1 , roach claim 1 , salmon claim 1 , sampa claim 1 , sauger claim 1 , sea bass claim 1 , seabream claim 1 , shiner claim 1 , sleeper claim 1 , snakehead claim 1 , snapper claim 1 , snook claim 1 , sole claim 1 , spinefoot claim 1 , sturgeon claim 1 , sunfish claim 1 , sweetfish claim 1 , tench claim 1 ...

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Номер записи: 31
17-02-2022 дата публикации

Stable Granules for Feed Compositions

Номер: US20220046955A1
Автор: Borup Flemming, Marcussen Erik Schmidt
Принадлежит: NOVOZYMES A/S

The present invention relates to pelletized feed compositions comprising granules which comprise an inner salt layer and an outer hydrophobic layer. The invention further relates to the use of salt and hydrophobic coated granules for steam treated pelletized feed compositions. 1. A pelletized feed composition comprising a granule comprising a core and a coating , wherein the core comprises a muramidase and the coating comprises an inner salt layer and an outer hydrophobic layer.2. The feed composition according to which has been exposed to steam treatment.3. The feed composition according to claim 1 , wherein the inner salt layer contributes between 30-60% w/w of the granule claim 1 , such as 40-60% w/w.4. The feed composition according to claim 1 , wherein the inner salt layer comprises NaSO claim 1 , KSO claim 1 , MgSOor a mixture thereof.5. The feed composition according to claim 1 , wherein the outer hydrophobic layer contributes between 2-3% w/w of the granule.6. The feed composition according to claim 1 , wherein the outer hydrophobic layer comprises hydrogenated palm oil.7. The feed composition according to claim 1 , wherein the muramidase is a GH25 muramidase which has ability to lyse bacterial cell walls.8. The feed composition according to claim 1 , wherein the humidity of the mash composition is at least 11% before pelleting and conditioning.9. The feed composition according to claim 1 , wherein the retained activity of the muramidase present in the core of the granules after steam pelleting at 85 degrees Celsius is at least 75% of the activity of the muramidase in the core of the granules before steam pelleting.10. A granule comprising a core and a coating claim 1 , wherein the core comprises a muramidase and the coating comprises an inner salt layer and an outer hydrophobic layer.11. A granule of claim 10 , wherein the granule comprises at least 75% of muramidase with retained activity after steam pelleting compared to the activity before steam ...

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Номер записи: 32
17-02-2022 дата публикации

Polypeptides Having Phytase Activity

Номер: US20220046956A1
Автор: Dietrich LÖBEL, Hanna-Mari MERILÄINEN, Imke KÜHN, Kari Juntunen, Marja Paloheimo, Merja Oja, Sandra MEYERJÜRGENS, Stefanie SCHOLTEN, Terhi Puranen, Thomas Haarmann
Принадлежит: AB ENZYMES GMBH, AB Enzymes Oy

The present invention discloses novel phytases that have improved phytase activity, compositions comprising them, recombinant host cells suitable for their production, and their use in feed applications.

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Номер записи: 33
17-02-2022 дата публикации

ANIMAL FEED COMPOSITION AND USE THEREOF

Номер: US20220047685A1
Автор: CARDOSO BITTENCOURT Leticia, LOPEZ-ULIBARRI Rual, PEREZ CALVO Estefania, RUBIO GARCIA Maria Elena
Принадлежит:

The present invention relates to a method of improving litter quality and/or reducing footpad dermatitis of a monogastric animal comprising administering to the animal a composition, an animal feed or an animal feed additive comprising one or more microbial muramidases. 1. A method of improving litter quality and/or reducing footpad dermatitis of a monogastric animal comprising administering to the animal a composition , an animal feed or an animal feed additive comprising one or more microbial muramidases.2. The method of claim 1 , wherein the monogastric animal is selected from the group consisting of swine claim 1 , piglet claim 1 , growing pig claim 1 , sow claim 1 , poultry claim 1 , turkey claim 1 , duck claim 1 , quail claim 1 , guinea fowl claim 1 , goose claim 1 , pigeon claim 1 , squab claim 1 , chicken claim 1 , broiler claim 1 , layer claim 1 , pullet and chick claim 1 , cat claim 1 , dog claim 1 , horse claim 1 , crustaceans claim 1 , shrimps claim 1 , prawns claim 1 , fish claim 1 , amberjack claim 1 , arapaima claim 1 , barb claim 1 , bass claim 1 , bluefish claim 1 , bocachico claim 1 , bream claim 1 , bullhead claim 1 , cachama claim 1 , carp claim 1 , catfish claim 1 , catla claim 1 , chanos claim 1 , char claim 1 , cichlid claim 1 , cobia claim 1 , cod claim 1 , crappie claim 1 , dorada claim 1 , drum claim 1 , eel claim 1 , goby claim 1 , goldfish claim 1 , gourami claim 1 , grouper claim 1 , guapote claim 1 , halibut claim 1 , java claim 1 , labeo claim 1 , lai claim 1 , loach claim 1 , mackerel claim 1 , milkfish claim 1 , mojarra claim 1 , mudfish claim 1 , mullet claim 1 , paco claim 1 , pearlspot claim 1 , pejerrey claim 1 , perch claim 1 , pike claim 1 , pompano claim 1 , roach claim 1 , salmon claim 1 , sampa claim 1 , sauger claim 1 , sea bass claim 1 , seabream claim 1 , shiner claim 1 , sleeper claim 1 , snakehead claim 1 , snapper claim 1 , snook claim 1 , sole claim 1 , spinefoot claim 1 , sturgeon claim 1 , sunfish claim 1 , ...

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Номер записи: 34
17-02-2022 дата публикации

PHYTASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

Номер: US20220049230A1
Автор: Santigosa Ester, Seon Aurelia Anne Catherine Charlotte, Skov Lars Kobberoe, Sorbara Jose-Otavio B., Vind Jesper, Walk Carrie Louise, Zhai Hengxiao, Zhang Qian
Принадлежит: NOVOZYMES A/S

The present invention relates to phytase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. 1. A phytase variant which has at least 70% identity to SEQ ID NO: 2 and which comprises the alterations N31C/G52C/A99C/K141C/T177C/V199C/N203L as compared to SEQ ID NO: 2 and further comprises a substitution in one or more position(s) selected from the following: 30 , 36 , 43 , 46 , 57 , 60 , 64 , 73 , 79 , 119 , 121 , 123 , 130 , 134 , 138 , 151 , 155 , 161 , 162 , 168 , 176 , 180 , 184 , 190 , 207 , 224 , 230 , 243 , 273 , 286 , 336 , 340 , 358 and 375 using SEQ ID NO: 2 for numbering.2. The variant of claim 1 , comprising substitutions in the positions: 57 claim 1 , 73 claim 1 , 121 claim 1 , 134 claim 1 , 155 claim 1 , 207 and 273.3. The variant of claim 2 , further comprising substitutions in the positions: 36 claim 2 , 60 claim 2 , 64 claim 2 , 73 claim 2 , 119 claim 2 , 130 claim 2 , 138 claim 2 , 161 claim 2 , 162 claim 2 , 168 claim 2 , 176 claim 2 , 180 claim 2 , 184 claim 2 , 190 claim 2 , 224 claim 2 , 230 claim 2 , 243 claim 2 , 336 and 340.4. The variant of claim 1 , where the substitutions are selected among: 30Q claim 1 , 36A claim 1 , 43C claim 1 , 46C claim 1 , 57Y claim 1 , 60H claim 1 , 64Q claim 1 , 73P claim 1 , 79Q claim 1 , 119P claim 1 , 121P claim 1 , 123C claim 1 , 130T claim 1 ,C claim 1 , 134Q claim 1 , 138A claim 1 , 151S claim 1 , 155F claim 1 , 161T claim 1 , 162A claim 1 , 168R 176P claim 1 , 180N claim 1 , 184Q claim 1 , 190T claim 1 , 207T claim 1 , 224Q claim 1 , 230E claim 1 , 243N claim 1 , 273L claim 1 , 286S claim 1 , 336R claim 1 , 340L claim 1 ,P claim 1 , 358Q and 375K.5. The variant of claim 1 , comprising the substitutions 31C/52C/57Y/73P/99C/121P/134Q/141C/155F/177C/199C/203L/207T/273L.6. The variant of claim 5 , selected among variants comprising the substitutions selected from ...

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Номер записи: 35
30-01-2020 дата публикации

Polypeptides having mannanase activity and polynucleotides encoding same

Номер: US20200032232A1
Автор: Kristian B.R.M. Krogh, Leigh Murphy, Nikolaj Spodsberg
Принадлежит: Novozymes AS

The present invention relates to polypeptides having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules.

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Номер записи: 36
24-02-2022 дата публикации

ANIMAL FEED COMPOSITIONS AND USES THEREOF

Номер: US20220054600A1
Автор: AURELI Raffaella, LOPEZ-ULIBARRI Rual, PEREZ CALVO Estefania
Принадлежит:

The present invention relates to a composition and/or an animal feed comprising polypeptides having muramidase activity and polypeptides having xylanase activity and uses thereof. 116-. (canceled)17. A method of improving flock uniformity and/or meat quality of a monogastric animal comprising administering to the animal a composition , an animal feed or an animal feed additive comprising one or more microbial muramidases.18. The method of claim 17 , wherein the monogastric animal is selected from the group consisting of swine claim 17 , piglet claim 17 , growing pig claim 17 , sow claim 17 , poultry claim 17 , turkey claim 17 , duck claim 17 , quail claim 17 , guinea fowl claim 17 , goose claim 17 , pigeon claim 17 , squab claim 17 , chicken claim 17 , broiler claim 17 , layer claim 17 , pullet and chick claim 17 , cat claim 17 , dog claim 17 , horse claim 17 , crustaceans claim 17 , shrimps claim 17 , prawns claim 17 , fish claim 17 , amberjack claim 17 , arapaima claim 17 , barb claim 17 , bass claim 17 , bluefish claim 17 , bocachico claim 17 , bream claim 17 , bullhead claim 17 , cachama claim 17 , carp claim 17 , catfish claim 17 , catla claim 17 , chanos claim 17 , char claim 17 , cichlid claim 17 , cobia claim 17 , cod claim 17 , crappie claim 17 , dorada claim 17 , drum claim 17 , eel claim 17 , goby claim 17 , goldfish claim 17 , gourami claim 17 , grouper claim 17 , guapote claim 17 , halibut claim 17 , java claim 17 , labeo claim 17 , lai claim 17 , loach claim 17 , mackerel claim 17 , milkfish claim 17 , mojarra claim 17 , mudfish claim 17 , mullet claim 17 , paco claim 17 , pearlspot claim 17 , pejerrey claim 17 , perch claim 17 , pike claim 17 , pompano claim 17 , roach claim 17 , salmon claim 17 , sampa claim 17 , sauger claim 17 , sea bass claim 17 , seabream claim 17 , shiner claim 17 , sleeper claim 17 , snakehead claim 17 , snapper claim 17 , snook claim 17 , sole claim 17 , spinefoot claim 17 , sturgeon claim 17 , sunfish claim 17 , sweetfish ...

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Номер записи: 37
07-02-2019 дата публикации

BIOACTIVE COMPOUNDS PROTECTION METHOD AND COMPOSITIONS CONTAINING THE SAME

Номер: US20190038560A1
Автор: BARZILAY Amir, Koren-Lichtig Hagit
Принадлежит:

The present invention relates to means for protecting bioactive materials in mammalian food or feed formulations used to enhance the health status of mammals. 123.-. (canceled)24. An encapsulated or embedded heat sensitive bioactive ingredient comprising:(i) a dry blend comprising insulin and maltodextrin and(ii) maltodextrin disposed over the dry blend, wherein the activity of the insulin is substantially maintained under dry conditions. This application is a divisional of U.S. patent application Ser. No. 10/561,541, filed Dec. 19, 2005, which is the U.S. National Stage of International Application No. PCT/IL2004/000532, filed Jun. 17, 2004, which claims the benefit of U.S. Provisional Application Nos. 60/548,164, filed Mar. 1, 2004, and 60/479,860 filed Jun. 20, 2003, the contents of each of which are incorporated herein by reference in their entireties.This invention relates generally to a method for the delivery of health promoting compounds to mammals and more specifically the invention relates to a method for encapsulating and embedding a bioactive ingredient in mammalian food or feed formulation.Bioactive proteins include EGF (Epidermal Growth Factor), insulin and insulin-like growth factors, insulin-like growth factors' binding proteins, immunoglobulins (e.g. antibody) proline-rich polypeptides, lactoferrin, proteases, lactalbumin, interleukin, lysozyme, TGFA (Transforming Growth Factor A) and PDGF (Platelet Derived Growth Factor).These proteins may have been shown to have a positive effect on one or more of the following health promotion advantages; protein efficiency ratios, weight gain, immune system functionality, proper growth of organs and cells, systemic and local DNA regulation, maintenance of good health, improvement of disease states and the recovery and cure from disease statesMost industrial processes, involve manufacturing conditions that are extremely destructive to potential viability of bioactive compounds like functional proteins. These ...

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Номер записи: 38
06-02-2020 дата публикации

POLYPEPTIDES HAVING MANNANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

Номер: US20200040320A1
Автор: Krogh Kristian B.R.M., Murphy Leigh, Spodsberg Nikolaj
Принадлежит: NOVOZYMES A/S

The present invention relates to polypeptides, isolated from , having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules. 1. A polypeptide having mannanase activity , selected from the group consisting of:(a) a polypeptide having at least 75% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, or (ii) the full-length complement of (i);(c) a polypeptide encoded by a polynucleotide having at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1;(d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has mannanase activity.2. The polypeptide of claim 1 , having at least 75% claim 1 , at least 80% claim 1 , at least 85% claim 1 , at least 90% claim 1 , at least 91% claim 1 , at least 92% claim 1 , at least 93% claim 1 , at least 94% claim 1 , at least 95% claim 1 , at least 96% claim 1 , at least 97% claim 1 , at least 98% claim 1 , at least 99% or 100% sequence identity to the mature polypeptide of SEQ ID NO: 2.3. The polypeptide of or claim 1 , which is encoded by a polynucleotide that hybridizes under low stringency conditions claim 1 , low-medium stringency conditions claim 1 , medium stringency conditions claim 1 , medium-high stringency conditions claim 1 , high stringency conditions claim 1 , or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID ...

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Номер записи: 39
14-02-2019 дата публикации

THERMOSTABLE PROTEASE AND METHODS OF MAKING AND USING THE SAME

Номер: US20190045813A1
Автор: Rupasinghe Sanjeewa, Wang Jeng-Jie
Принадлежит:

Proteolytic enzyme variants that exhibit improved thermal stability relative to the wild type protease PWD-1 protease kerA are provided. The protease variants are derived by the mutation of at least one amino acid residue of the mature PWD-1 protease kerA. The variant proteases are useful as animal feed additives to improve the digestibility and/or the nutritional value of the animal feed. 1Bacillus licheniformis. A polypeptide variant of kerA protease , the variant protease comprising a sequence as set forth in:a. A298P:N322S:S363D (SEQ ID NO: 1), N322S (SEQ ID NO: 2), G222S: N265C: N322S (SEQ ID NO: 3), P145E: A298P: N322S: S363D (SEQ ID NO: 4), N322S: S363D (SEQ ID NO: 5), P145E: N322S (SEQ ID NO: 6), N265C: N322S (SEQ ID NO: 7), P145E: A298P:S363D (SEQ ID NO: 8), N265C (SEQ ID NO: 9), G222S: N265C (SEQ ID NO: 10), G222S: N227Y (SEQ ID NO: 11), N265C: G270R (SEQ ID NO: 12), P145E: S363D (SEQ ID NO: 13), or G222S: N227Y: N265C A298P (SEQ ID NO: 14);{'i': 'Bacillus licheniformis', 'b. the variant sequence as set forth in part (a) and having at least 70% sequence identity in a remainder of the sequence, wherein the variant sequence has protease activity and retains enhanced thermostability relative to kerA protease;'}c. the variant sequence as set forth in part (b), wherein the at least 70% sequence identity consists of only conservative substitutions; or{'i': 'Bacillus licheniformis', 'd. a fragment of the variant sequence as set forth in parts (a), (b), or (c), wherein the fragment has protease activity and retains enhanced thermostability relative to kerA protease.'}2. The variant protease of claim 1 , wherein the at least 70% sequence identity comprises at least 85% identity claim 1 , at least 90% identity claim 1 , at least 95% identity claim 1 , at least 98% identity claim 1 , or at least 99% identity.3. A polynucleotide encoding the variant protease of .4. A nucleic acid construct or expression vector comprising the polynucleotide of claim 3 , wherein the ...

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Номер записи: 40
22-02-2018 дата публикации

COMPOSITIONS AND METHODS COMPRISING VARIANT MICROBIAL PROTEASES

Номер: US20180051269A1
Автор: Basler Joshua R., Cascao-Pereira Luis G., Estell David A., JR. James T., Kellis
Принадлежит:

The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications. 1. An isolated subtilisin variant comprising at least one set of the following substitution sets: G97A-G128A-Y217Q , G97A-L126A-G128A , G97A-L126A-G128A-Y217Q , G97A-L126A-Y217Q , G97A-M124V-G128A , G97A-M124V-G128A-Y217Q , G97A-M124V-L126A , G97A-M124V-L126A-G128A , G97A-M124V-L126A-Y217Q , G97A-M124V-Y217Q , G97A-N123G-G128A , G97A-N123G-G128A-Y217Q , G97A-N123G-L126A , G97A-N123G-L126A-G128A , G97A-N123G-L126A-Y217Q , G97A-N123G-M124V , G97A-N123G-M124V-G128A , G97A-N123G-M124V-L126A , G97A-N123G-M124V-Y217Q , G97A-N123G-Y217Q , L126A-G128A-Y217Q , L96T-G128A-Y217Q , L96T-G97A-G128A , L96T-G97A-G128A-Y217Q , L96T-G97A-L126A , L96T-G97A-L126A-G128A , L96T-G97A-L126A-Y217Q , L96T-G97A-M124V , L96T-G97A-M124V-G128A , L96T-G97A-M124V-L126A , L96T-G97A-M124V-Y217Q , L96T-G97A-N123G , L96T-G97A-N123G-G128A , L96T-G97A-N123G-L126A , L96T-G97A-N123G-M124V , L96T-G97A-N123G-Y217Q , L96T-G97A-Y217Q , L96T-L126A-G128A , L96T-L126A-G128A-Y217Q , L96T-L126A-Y217Q , L96T-M124V-G128A , L96T-M124V-G128A-Y217Q , L96T-M124V-L126A , L96T-M124V-L126A-G128A , L96T-M124V-L126A-Y217Q , L96T-M124V-Y217Q , L96T-N123G-G128A , L96T-N123G-G128A-Y217Q , L96T-N123G-L126A , L96T-N123G-L126A-G128A , L96T-N123G-L126A-Y217Q , L96T-N123G-M124V , L96T-N123G-M124V-G128A , L96T-N123G-M124V-L126A , L96T-N123G-M124V-Y217Q , L96T-N123G-Y217Q , M124V-G128A-Y217Q , M124V-L126A-G128A , M124V-L126A-G128A-Y217Q , M124V-L126A-Y217Q , N123G-G128A-Y217Q , N123G-L126A-G128A , N123G-L126A-G128A-Y217Q , N123G-L126A-Y217Q , N123G-M124V-G128A , N123G-M124V-G128A-Y217Q , N123G-M124V-L126A , N123G-M124V-L126A-G128A , N123G-M124V-L126A-Y217Q , N123G-M124V-Y217Q , N62Q-G128A-Y217Q , N62Q-G97A-G128A , N62Q-G97A-G128A- ...

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Номер записи: 41
26-02-2015 дата публикации

Compositions and Methods Comprising Variant Microbial Proteases

Номер: US20150056682A1
Автор: Alexander Pisarchik, James T. Kellis, Jr., Luis G. Cascao-Pereira, Viktor Yuryevich ALEKSEYEV
Принадлежит: DANISCO US INC

The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant proteases suitable for laundry cleaning applications.

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Номер записи: 42
02-03-2017 дата публикации

SYSTEM FOR AND METHOD OF CONVERTING AGRICULTURAL WASTE TO PROBIOTIC ANIMAL FEED

Номер: US20170055545A1
Автор: Lee Chie Ying
Принадлежит:

Methods of and devices for producing probiotic animal feed are provided. The method includes adding probiotic microorganisms or enzymes, providing an environmental condition suitable for the growth of the probiotic microorganisms, such as providing abundant sources of carbon, controlling temperature, and controlling pH values, in a fermenting process. 1. A method of making probiotic animal feed comprising:a) adding one or more probiotic substances to a fermenting; andb) generating a probiotic animal feed.2. The method of claim 1 , further comprising breaking cell wall of an agriculture substance before fermenting.3. The method of claim 1 , further comprising separating a liquid portion from a solid portion claim 1 , wherein the liquid portion is sent to the fermenting.4. The method of claim 1 , wherein the probiotic substances comprise microorganisms.5. The method of claim 1 , wherein the probiotic substances comprise enzymes of microorganisms.6. The method of claim 1 , wherein the probiotic substances comprise enzymes.7. The method of claim 1 , further comprising converting hydrocarbons to organic acids.8Bacillus subtilisLactobacillus acidophilus.. The method of claim 1 , further comprising co-fermenting with an engineered and engineered9. A method of making probiotic animal feed comprising:a) providing an environment suitable for the proliferation of probiotic microorganisms; andb) generating a probiotic animal feed.10. The method of claim 9 , further comprising increasing the number of probiotic microorganisms.11. The method of claim 9 , further comprising a fiber and liquid separating claim 9 , which generates a fiber portion and a liquid portion.12. The method of claim 11 , further comprising adjusting a pH value of the liquid portion to a range between 5.5 and 7.13. The method of claim 11 , further comprising precipitating proteins and nutrients by performing the pH adjusting.14. A method of making probiotic animal feed comprising:a) collecting an agriculture ...

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Номер записи: 43
20-02-2020 дата публикации

SKELETAL MUSCLE HYPERTROPHY INDUCERS

Номер: US20200054649A1
Автор: DUCHEMIN-PELLETIER EVE, FLAENDER MÉLANIE, Michaud Joris, POYDENOT PAULINE, SELIG LUC
Принадлежит:

The present invention relates to skeletal muscle hypertrophy inducers as well as their uses to promote skeletal muscle regeneration, to prevent skeletal muscle atrophy, or in the treatment or prevention of a disease or injury resulting in loss of skeletal muscle tissue and/or muscle weakness. It further relates to the non-therapeutic use of such compounds to increase muscle mass, muscle strength and/or muscle performance in a subject. 147-. (canceled)50. The method according to claim 48 , wherein the compound of formula (I):wherein:{'sub': 1', '1', '3, 'Ris hydrogen or a C-Calkyl, or is absent;'}{'sub': '2', 'Ris selected from the group consisting of hydrogen, a hydroxyl group, a 4-dimethylamino-phenyl group, a 4-methylamino-phenyl group and an aminophenyl group;'}{'sub': 3', '8', '8', '1', '3', '2, 'Ris a hydroxyl group or —C(═O)Rwith Rbeing a C-Calkyl or —CHOH;'}{'sub': '4', 'Ris hydrogen, an acetoxy group or a propynyl group;'}{'sub': 5', '6, 'Rand Rare hydrogen or taken together form a methylene group;'}{'sub': '7', 'Ris hydrogen or methyl;'}{'sub': '1', 'a and b respectively denote, independently from each other, a single bond or a double bond, with the proviso that Ris absent when a is a double bond;'}{'sub': 4', '1', '1', '3', '7', '2', '3', '8', '8', '2', '4', '3, 'with the provisos that (i) Ris an acetoxy group or a propynyl group and Ris a C-Calkyl when Ris methyl, (ii) Ris selected from the group consisting of a hydroxyl group, a 4-dimethylamino-phenyl group, a 4-methylamino-phenyl group and an aminophenyl group when Ris —C(═O)Rwith Rbeing —CHOH and (iii) Ris an acetoxy group or a propynyl group when Ris a hydroxyl group;'}or any pharmaceutically acceptable, salt, hydrate, solvate or prodrug thereof.51. The method according to claim 48 , wherein:{'sub': '1', 'Ris hydrogen or methyl, or is absent;'}{'sub': '2', 'Ris selected from the group consisting of hydrogen, a hydroxyl group and a 4-dimethylamino-phenyl group;'}{'sub': 3', '8', '8', '2, 'Ris a ...

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Номер записи: 44
04-03-2021 дата публикации

HEMICELLULASE

Номер: US20210062173A1
Автор: Hagiwara Yusuke, Mihara Yasuhiro, NAKAMURA Satoshi, Takagi Tomo, Yakushijin Rie
Принадлежит: AJINOMOTO CO., INC.

Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described. 1. A composition comprising a first protein and a second protein , said first and second proteins are each selected from the group consisting of protein (A) , protein (B) , protein (C) , and protein (D);wherein protein (A) is selected from the group consisting of:(A1) a protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO: 4, positions 1 to 536 of SEQ ID NO: 26, positions 1 to 536 of SEQ ID NO: 28, or positions 1 to 536 of SEQ ID NO: 30, wherein said protein has hemicellulase activity;(A2) a protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO: 4, positions 1 to 536 of SEQ ID NO: 26, positions 1 to 536 of SEQ ID NO: 28, or positions 1 to 536 of SEQ ID NO: 30, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said protein has hemicellulase activity; and(A3) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 535 of SEQ ID NO: 4, positions 1 to 536 of SEQ ID NO: 26, positions 1 to 536 of SEQ ID NO: 28, or positions 1 to 536 of SEQ ID NO: 30, wherein said protein has hemicellulase activity;wherein protein (B) is selected from the group consisting of:(B1) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO: 12, positions 1 to 529 of SEQ ID NO: 32, or positions 1 to 390 of SEQ ID NO: 34, wherein said protein has hemicellulase activity,(B2) a protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO: 12, positions 1 to 529 of SEQ ID NO: 32, or positions 1 to 390 of SEQ ID NO: 34, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein ...

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Номер записи: 45
05-03-2015 дата публикации

Compositions and methods comprising variant microbial proteases

Номер: US20150064767A1
Автор: David A. Estell, James T. Kellis, Joshua R. Basler, Luis G. Cascao-Pereira
Принадлежит: DANISCO US INC

The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.

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Номер записи: 46
17-03-2022 дата публикации

STABLE DRY POWDER COMPOSITION COMPRISING BIOLOGICALLY ACTIVE MICROORGANISMS AND/OR BIOACTIVE MATERIALS AND METHODS OF MAKING

Номер: US20220081471A1
Автор: Artimovich Elena, Carpenter Brian, Drewes Roger, Harel Mordechai
Принадлежит: ADVANCED BIONUTRITION CORP.

The present invention relates to embedding live or dead microorganisms and/or bioactive materials in a protective dry formulation matrix, wherein the formulation includes the bioactive microorganism or material, a formulation stabilizer agent, and a protective agent. The formulation is prepared by dispersing all the solid components in a solution, with or without a vacuum, and cooling the solution to a temperature above its freezing temperature. The methods include a primary drying step of the formulation at a desired temperature and time period, and an accelerated secondary drying step under maximum vacuum and elevated temperature, to achieve a final desirable water activity of the dry material. 1. A composition comprising (i) a bioactive microorganism or material as fresh , frozen or dry powder , (ii) at least two stabilizer agents , and (iii) at least two protective agents , wherein the composition is suitable for a liquid state drying and stabilizing the bioactive microorganism or material.2. The composition of claim 1 , wherein total solids range from about 30 weight percent to about 70 weight percent.3. The composition of claim 1 , wherein the bioactive microorganism or material is selected from a cell claim 1 , a microbe claim 1 , a virus claim 1 , a culture claim 1 , a probiotic claim 1 , a yeast claim 1 , an algae claim 1 , a protein claim 1 , a recombinant protein claim 1 , an enzyme claim 1 , a peptide claim 1 , a hormone claim 1 , a vaccine claim 1 , a drug claim 1 , a vitamin claim 1 , a mineral claim 1 , a microbiocide claim 1 , a fungicide claim 1 , a herbicide claim 1 , an insecticide or a spermicide.4. The composition according to claim 1 , wherein the stabilizer agent is a polysaccharide and or an oligosaccharide.5. The composition of claim 4 , wherein the polysaccharides is selected from cellulose acetate phthalate (CAP) claim 4 , carboxy-methyl-cellulose claim 4 , pectin claim 4 , sodium alginate claim 4 , salts of alginic acid claim 4 , hydroxyl ...

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Номер записи: 47
28-02-2019 дата публикации

Amylases, Nucleic Acids Encoding Them and Methods for Making and Using Them

Номер: US20190062715A1
Автор: Aboushadi Nahla M., Garrett James B., Gray Kevin A.
Принадлежит:

In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars. 1. A method for hydrolyzing a starch comprising: (a) providing a starch; (b) providing a polypeptide having glucoamylase activity , wherein the polypeptide is at least 80% identical to the amino acid sequence of SEQ ID NO:2 , SEQ ID NO: 4 , SEQ ID NO:6 , SEQ ID NO:10 , SEQ ID NO:12 , SEQ ID NO:14 , or SEQ ID NO: 16 , contacting the starch of (a) with the polypeptide of (b) wherein the polypeptide hydrolyzes the starch.2. The method of claim 1 , wherein the starch is from rice claim 1 , corn claim 1 , barley claim 1 , wheat claim 1 , legumes claim 1 , or potato.3. The method of claim 1 , wherein the hydrolyzing a starch is an animal feed product.4. The method of claim 1 , wherein the hydrolyzing a starch is a food product.5. The method of claim 1 , wherein the hydrolyzing the starch is for desizing the fabric.6. The method of claim 1 , wherein the hydrolyzing starch for paper processing.7. A method of claim 1 , wherein the hydrolyzing starch produces a high-maltose or a high-glucose syrup.8. A method of claim 1 , wherein hydrolyzing starch in a baked product prevents staling of the baked product.9. The method as set forth in claim 8 , wherein the baked product is bread.10. A method of claim 1 , wherein the hydrolyzing starch for alcohol production.11. A method of claim 1 , wherein the hydrolyzing starch for a brewing process.12. The method as set forth in claim 11 , wherein the composition comprising the starch is in a wort used for brewing beer.13. The method of claim 1 , wherein the hydrolyzing starch for saccarification. This application is a divisional of U.S. patent application Ser. No. 14/971,255, filed Dec. 16, 2015, now U.S. Pat. No. 10,100,293, granted on ...

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Номер записи: 48
11-03-2021 дата публикации

GRANULATE COMPRISING AN ENZYME, A CARRIER AND A VEGETABLE OIL

Номер: US20210068438A1
Автор: BEEKMAN Willem Johan, KERKHOF Pieter Theodorus, MEESTERS Gabriel Marinus Henricus
Принадлежит:

The present invention relates to an enzyme granulate comprising a carrier and an enzyme, and wherein the granulate comprises from 0.015 to 0.4 wt % of vegetable oil, and a process for preparing an enzyme granulate, comprising mixing a carrier with an enzyme and adding a composition comprising a vegetable oil, wherein the enzyme granulate comprises from 0.015 wt % to 0.4 wt % of the vegetable oil. The invention further relates to the use of the enzyme granulate in the preparation of a food or feed product. 1. An enzyme granulate comprising a carrier and an enzyme , and wherein the granulate comprises from 0.015 to 0.4 wt % of vegetable oil.2. The enzyme granulate according to claim 1 , wherein the carrier comprises wheat flour claim 1 , maltodextrin claim 1 , agglomerated maltodextrin claim 1 , or rice flour.3. The enzyme granulate according to claim 1 , wherein the enzyme is an amylase claim 1 , pectinase claim 1 , protease claim 1 , a lipase claim 1 , a xylanase claim 1 , a cellulase claim 1 , asparginase and/or a glucose oxidase.4. The enzyme granulate according to claim 1 , wherein the vegetable oil comprises sunflower oil claim 1 , palm oil claim 1 , soy oil and/or canola oil.5. The enzyme granulate according to claim 1 , wherein from 60% to 100% of the granulate has a particle size of between 63 to 224 μm based on sieve analysis.6. A process for preparing an enzyme granulate claim 1 , comprising mixing a carrier with an enzyme and adding a composition comprising a vegetable oil claim 1 , wherein the enzyme granulate comprises 0.015 wt % to 0.4 wt % of the vegetable oil.7. The process according to claim 6 , wherein adding a composition comprising a vegetable oil is performed before claim 6 , during or after the mixing of a carrier with an enzyme.8. The process according to claim 6 , wherein the composition comprising the vegetable oil is a liquid vegetable oil9. The process according to claim 6 , wherein the composition comprising a vegetable oil is a second ...

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Номер записи: 49
12-03-2015 дата публикации

Multiple Mutation Variants of Serine Protease

Номер: US20150072913A1
Автор: Andrew Shaw, Ayrookaran J. Poulose, Brian E. Jones, Chris Leeflang, David A. Estell, Hiroshi Oh, Leonardus P.M. Van Marrewijk, Marc Kolkman, Ronaldus W.J. Hommes, Wilhelmus A.H. Van der Kley, Wolfgang Aehle
Принадлежит: DANISCO US INC

The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.

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Номер записи: 50
29-05-2014 дата публикации

Utilization of an enzymatic complex in farm animals feed

Номер: US20140147550A1
Автор: Dominique Hooreman
Принадлежит: Denis HOOREMAN, Herve HOOREMAN, Jean-Noel HOOREMAN

The invention relates to a method of supplementing farm animal feed with an enzymatic complex including a mixture of proteases, obtained by culturing a Streptomyces fradiae strain, wherein among this mixture of proteases one of them has an isoelectric point of around 7.0 and another has an isoelectric point of around 8.0. The invention also relates to feed compositions for farm animals including the enzymatic complex as well as the manufacturing process enzymatic complex thereof.

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Номер записи: 51
15-03-2018 дата публикации

Amino acid animal feed composition

Номер: US20180070611A1
Автор: Feng Wan, Timothy Martin LONDERGAN
Принадлежит: Benemilk Oy, Feng Wan, Timothy Martin LONDERGAN

A rumen by-pass composition for a ruminant includes an amine compound, and a fatty acid component, comprising an organic acid, wherein the amine compound and the organic acid are configured to form an amine-organic acid salt, and wherein the fatty acid component has a melting point not less than 40° C. and an Iodine Value not greater than 45.

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Номер записи: 52
24-03-2022 дата публикации

PROCESS OF SPRAY DRYING OF GLP-1 PEPTIDE

Номер: US20220088500A1
Автор: Ingvarsson Pall Thor
Принадлежит:

The present invention relates to a process for spray drying of a feed solution comprising the GLP-1 peptide semaglutide wherein the ratio of atomising gas flow (kg/h) to feed solution flow (kg/h) is 1.0-1.7. 1. A process for spray drying of a feed solution comprising semaglutide , the process comprising introducing the feed solution comprising semaglutide in a solvent into a spray dryer at a feed flow rate and introducing an atomising gas at an atomising gas flow rate , wherein the ratio of atomising gas flow rate (kg/h) to feed flow rate (kg/h) is in the range 1.0-1.7.2. The process according to claim 1 , wherein the ratio of atomising gas flow rate (kg/h) to feed flow rate (kg/h) is in the range 1.1-1.7.3. The process according to claim 1 , wherein the feed flow rate is 20-60 kg/h.4. The process according to claim 1 , wherein the feed flow rate is 41-51 kg/h.5. The process according to claim 1 , wherein the atomising gas flow rate is 20-80 kg/h.6. The process according to claim 1 , wherein the atomising gas flow rate is 52-62 kg/h.7. The process according to claim 1 , wherein the feed solution comprises 0.5-10% (w/w) semaglutide in 40-70% (w/w) aqueous organic solvent.8. The process according to claim 7 , wherein the aqueous organic solvent is selected from the list consisting of aqueous ethanol and aqueous acetonitrile.9. The process according to claim 1 , wherein the outlet temperature is 50-100° C.10. The process according to claim 1 , wherein the outlet temperature is 60-90° C.11. The process according to claim 1 , wherein the inlet temperature is 85-200° C.12. The process according to claim 1 , wherein the drying gas flow rate is 1000-1800 kg/h.13. The process according to claim 1 , wherein the nozzle used has at least two-fluid entry channels.14. The process according to claim 1 , wherein the nozzle used is a two-fluid nozzle.15. The process according to claim 1 , wherein the feed solution consists essentially of 2.0-2.2% (w/w) semaglutide in 50-55% (w/w) ...

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Номер записи: 53
15-03-2018 дата публикации

Fungus-Sourced High-Temperature Acid B-Glucosidase as well as Coding Gene and Application Thereof

Номер: US20180073004A1
Автор: BAI Yingguo, HUANG Huoqing, LUO Huiying, Ma Rui, SHI PENGJUN, SHI XIA, SU Xiaoyun, WANG Yaru, Xia Wei, YANG PEILONG, Yao Bin
Принадлежит:

Provided are a fungus-sourced high-temperature acid β-glucosidase as well as a coding gene, and an application thereof. The provide β-glucosidase has the optimal pH value of 4.5 and the optimal temperature of 75° C., and maintains over 90% enzyme activity in the optimal condition after being processed at 60° C. for 1 h. The re-engineering yeast strain GS115/bgl3A of the coding gene comprising the β-glucosidase has high fermentation level. 1. A high-temperature acid β-glucosidase which is selected from:(a) a polypeptide comprising the amino acid as shown in SEQ ID NO: 1 or SEQ ID NO: 2;(b) a polypeptide with β-glucosidase activity which is derived from SEQ ID NO: 1 or SEQ ID NO. 2 by substitution, deletion and/or insertion of one or more amino acid residues.2. An isolated polynucleotide with the following characteristics:(a) coding a polypeptide comprising the amino acid as shown in SEQ ID NO. 1 or SEQ ID NO. 2;(b) coding a polypeptide with β-glucosidase activity which is derived from SEQ ID NO: 1 or SEQ ID NO. 2 by substitution, deletion and/or insertion of one or more amino acid residues.3. The isolated polynucleotide according to which is selected from:(a) DNA comprising a nucleotide sequence set in forth in SEQ ID NO.4 or SEQ ID NO.5; or(b) DNA hybridizing under stringent conditions, to a nucleotide sequence set in forth in SEQ ID NO.4 or SEQ ID NO.5, and coding polypeptide with glucosidase activity.4. A DNA construct or a recombinant vector comprising the polynucleotide of .5. A recombinant vector pPIC9-bgl3A comprising the polynucleotide of .6. A recombinant host cell comprising the polynucleotide of .7. A recombinant host cell GS115/bgl3 comprising the polynucleotide of .8. A method of producing a high-temperature acid β-glucosidase comprising the steps of:{'claim-ref': {'@idref': 'CLM-00004', 'claim 4'}, '(1) transforming a host cell with the DNA construct or a recombinant vector of to obtain the recombinant host cell;'}(2) cultivating the recombinant host ...

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Номер записи: 54
07-03-2019 дата публикации

NOVEL PHYSTASE, METHOD FOR OBTAINING THE SAME AND USE THEREOF

Номер: US20190071654A1
Автор: ALIGUE Rosa, ATARES-REAL Sergio, MARQUES-MASCARELL Ramón, MARTIN-PEREZ Julia, ROMERO-LOPEZ Joaquín, SALAET-MADORRAN Ignasi
Принадлежит: Fertinagro Nutrientes, S.L.

The invention generally relates to novel and improved nucleic acid sequences encoding a polypeptide or protein having the enzymatic activity of a phytase and methods for effectively expressing such enzymes in a host cell. In a first aspect, the present invention relates an isolated nucleic acid molecule i) comprising the nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity or ii) having a sequence identity of at least 83% to a nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity. The present invention also relates to an expression construct or a vector comprising the above mentioned isolated nucleic acid molecule. The present invention further relates to a method of producing a protein having the enzymatic activity of a phytase comprising the steps of: a) introducing into a host cell a vector comprising: i) elements regulating the expression that are functional in the host cell; and ii) operatively linked thereto a nucleic acid molecule according to the invention; b) cultivating the host cells obtained in step a) under conditions suitable for expression of the protein, and optionally c) recovering the protein produced in step b) from the cell culture. The present invention further relates to proteins obtained by the method according to the invention and the use thereof for the manufacture of additives for animal feed. 1. An isolated nucleic acid moleculei) comprising the nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity orii) having a sequence identity of at least 83% to a nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity.2. The isolated nucleic acid molecule according to claim 1 , wherein the nucleic acid molecule does not comprise a nucleotide sequence encoding for a N-terminal signal peptide.3. The isolated nucleic acid molecule according to claim 1 , further comprising codon-optimizing mutations with respect to a host cell ...

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Номер записи: 55
18-03-2021 дата публикации

ANIMAL FEED COMPOSITIONS AND USES THEREOF

Номер: US20210076704A1
Автор: AURELI Raffaella, LOPEZ-ULIBARRI Rual, PEREZ CALVO Estefania
Принадлежит:

The present invention relates to animal feed compositions comprising polypeptides having muramidase activity and polypeptides having xylanase activity and uses thereof. 1. An animal feed comprising an animal feed additive , one or more protein sources and one or more energy sources characterised in the animal feed or the animal feed additive further comprises one or more polypeptides having xylanase activity and one or more fungal polypeptides having muramidase activity.2. The animal feed of claim 1 , wherein the polypeptide having muramidase activity is a fungal GH24 muramidase or GH25 muramidase.3. The animal feed of claim 1 , wherein the polypeptide having xylanase activity is a GH10 xylanase claim 1 , GH11 xylanase claim 1 , GH5 xylanase or GH30 xylanase.4Lactobacillus johnsonii.. The animal feed of claim 1 , wherein the polypeptide having muramidase activity degrades cell wall debris from5. The animal feed of claim 1 , wherein the polypeptide having muramidase activity is selected from the group consisting of:(a) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 1;(b) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 2;(c) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 3;(d) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 4;(e) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 5;(f) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 6;(g) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 95%, or 100% sequence identity to SEQ ID NO: 7;(h) a polypeptide having at least 80%, e.g., at least 85%, at least ...

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Номер записи: 56
05-03-2020 дата публикации

NOVEL FUNGAL FUCOSIDASES AND THEIR USE IN PREVENTING AND/OR TREATING A PATHOGENIC INFECTION IN AN ANIMAL

Номер: US20200071685A1
Автор: Zhang Zhenghong
Принадлежит:

Disclosed are methods and compositions using glycoside hydrolases, such as an alpha-L-fucosidases, to prevent and/or treat a pathogenic infection and/or diarrhea in an animal wherein the pathogenic infection is caused by a pathogen capable of binding to an animal intestinal cell wherein said binding of the pathogen is dependent on the presence of a pathogen binding site having at least one glycan structure substituted with at least one alpha-1,2-L-fucose moiety comprising administering to the animal an effective amount of a glycoside hydrolase capable of removing the at least one alpha-1,2-L-fucose moiety from the pathogen binding site. 1. An isolated polypeptide having alpha-fucosidase activity , selected from the group consisting of:a) a polypeptide having a predicted mature amino acid sequence of at least 93% identity with the amino acid sequence of SEQ ID NO:22;b) a polypeptide having a predicted mature amino acid sequence of at least 90% identity with the amino acid sequence of SEQ ID NO:23;c) a polypeptide having a predicted mature amino acid sequence of at least 75% identity with the amino acid sequence of SEQ ID NO:24;d) a polypeptide having a predicted mature amino acid sequence of at least 70% identity with the amino acid sequence of SEQ ID NO:25;e) a polypeptide having a predicted mature amino acid sequence of at least 95% identity with the amino acid sequence of SEQ ID NO:26;f) a polypeptide having a predicted mature amino acid sequence of at least 71% identity with the amino acid sequence of SEQ ID NO:27; andg) a polypeptide having a predicted mature amino acid sequence of at least 97% identity with the amino acid sequence of SEQ ID NO:28.2. An isolated polypeptide having alpha-fucosidase activity which is comprised within a predicted precursor amino acid sequence selected from the group consisting of: SEQ ID NO:15; SEQ ID NO:16; SEQ ID NO:17; and SEQ ID NO:18; SEQ ID NO:19; SEQ ID NO:20; and SEQ ID NO:21.3. A recombinant construct comprising a ...

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Номер записи: 57
24-03-2016 дата публикации

Method for the preparation of an enzyme tablet

Номер: US20160081356A1
Автор: Jens DAMGAARD
Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

The present invention relates to a method for the preparation of an enzyme tablet comprising a high amount of enzyme (having a high pay-load) for use in for example the feed or food industry.

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Номер записи: 58
14-03-2019 дата публикации

FEED ADDITIVE COMPOSITION

Номер: US20190076488A1
Автор: Davis Mari Ellen, MILAN LUIS FERNANDO ROMERO, SIRAGUSA GREGORY ROSS, Smith Alexandra Helena
Принадлежит:

A feed additive composition comprising a direct fed microbial in combination with a protease, a xylanase, an amylase and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for avoiding the negative effects of necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease, a xylanase, an amylase and a phytase. 1. A feed additive composition comprising a direct fed microbial in combination with a protease , a xylanase , an amylase and a phytase.2. A feed additive composition according to wherein the direct fed microbial is an antipathogen direct fed microbial.3. A feed additive composition according to or wherein the direct fed microbial is a viable bacterium.4Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, ClostridiumMegasphaera. A feed additive composition according to any one of to wherein the direct fed microbial comprises a bacterium from one or more of the following genera: and and combinations thereof.5Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus, EnterococcusPediococcusLactobacillusBifidobacteriumLactobacillus acidophilus, Pediococsus acidilactici, Lactococcus lactis, Bifidobacterium bifidum, Propionibacterium thoenii, Lactobacillus farciminus, lactobacillus rhamnosus, Clostridium butyricum, Bifidobacterium animalisanimalis, Lactobacillus reuteri, Bacillus cereus, Lactobacillus salivariussalivarius ...

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Номер записи: 59
31-03-2022 дата публикации

ENGINEERED PHYTASES AND METHODS OF USING THE SAME

Номер: US20220095647A1
Автор: Lazar Gabor, Raab R. Michael, Shen Binzhang
Принадлежит: AGRIVIDA, INC.

Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described. 1. An animal feed comprising an engineered phytase comprising a target phytase , a first binding element and a second binding element , wherein each of the first binding element and the second binding is fused to the target phytase , the first binding element interacts with the second binding element to cause cyclization of the engineered phytase , and enhance thermal stability of the target phytase , and wherein the first binding element or the second binding element comprises an intein or part thereof.2. The animal feed of claim 1 , wherein upon the interaction claim 1 , each of the first binding element and the second binding element is capable of being released from the engineered phytase spontaneously.3. The animal feed of claim 1 , wherein the first binding element or the second binding element is fused to the N-terminus or the C-terminus of the target phytase.4. The animal feed of claim 3 , wherein the C-terminus of the first binding element is linked to and contiguous with the N-terminus of the target phytase.5. The animal feed of claim 3 , wherein the N-terminus of the second binding element is linked to and contiguous with the C-terminus of the target phytase.6Escherichia coli, Aspergillus niger, Peniophora lycii, Neurospora crassaSchwaniomyces accidentalis.. The animal feed of claim 1 , wherein the target phytase is selected from the group consisting of phytases derived from claim 1 , and7. The animal feed of claim 1 , ...

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Номер записи: 60
22-03-2018 дата публикации

NOVEL METALLOPROTEASES

Номер: US20180080017A1
Автор: BABE Lilia Maria, Ghirnikar Roopa Santosh, Goedegebuur Frits, Gu Xiaogang, KOLKMAN MARC ANTON BERNHAR, Yao Jian, Yu Shukun
Принадлежит:

Aspects of the present compositions and methods relate to novel metalloproteases polynucleotides encoding the novel metalloprotease, compositions and methods for use thereof. 1. A polypeptide comprising an amino acid sequence having at least 60% sequence identity to the amino acid sequence selected from the group consisting of of SEQ ID NOs: 3 , 6 , 9 and 14.2. The polypeptide of claim 1 , wherein said polypeptide has at least 80% sequence identity to the amino acid sequence selected from the group consisting of of SEQ ID NOs: 3 claim 1 , 6 claim 1 , 9 and 14.3. The polypeptide of of any of or claim 1 , wherein said polypeptide has at least 95% sequence identity to the amino acid sequence selected from the group consisting of of SEQ ID NOs: 3 claim 1 , 6 claim 1 , 9 and 14.4. The polypeptide of of any of to claim 1 , wherein said polypeptide has at least 98% sequence identity to the amino acid sequence selected from the group consisting of of SEQ ID NOs: 3 claim 1 , 6 claim 1 , 9 and 14.5. The polypeptide of any of the above claims claim 1 , wherein said amino acid sequence is the amino acid sequence of of SEQ ID NO: 3.6. The polypeptide of any of the above claims claim 1 , wherein said amino acid sequence is the amino acid sequence of of SEQ ID NO: 6.7. The polypeptide of any of the above claims claim 1 , wherein said amino acid sequence is the amino acid sequence of of SEQ ID NO: 9.8. The polypeptide of any of the above claims claim 1 , wherein said amino acid sequence is the amino acid sequence of of SEQ ID NO: 14.9. The polypeptide of any of the above claims claim 1 , wherein said polypeptide is derived from a member of the order Bacillales.10Bacillus, Brevibacillus, LysinibacillusAnoxybacillus. The polypeptide of any of the above claims claim 1 , wherein said Bacillales member is a or spp.11Anoxybacillus, LysinibacillusBrevibacillusAnoxybacillus rupiensis, Lysinibacillus macroidesBrevibacillus brevis. The polypeptide of claim 10 , wherein said or are claim 10 , ...

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Номер записи: 61
25-03-2021 дата публикации

ANIMAL FEED COMPOSITIONS AND METHODS OF USE

Номер: US20210084939A1
Автор: Drouillard James S., Horton Lucas Michael
Принадлежит: SYNGENTA PARTICIPATIONS AG

The invention provides an animal feed composition comprising plant material from a transgenic plant or plant part expressing a recombinant thermotolerant α-amylase. The invention further provides methods of improving feed utilization and decreasing liver abscesses. Also provided are methods of producing a steam flaked corn product, and the steam flaked corn product so produced. The invention provides an animal feed composition comprising plant material from a transgenic plant or plant part expressing a recombinant thermotolerant α-amylase. The invention further provides methods of improving feed utilization and decreasing liver abscesses. Also provided are methods of producing a steam flaked corn product, and the steam flaked corn product so produced. 1. A method of reducing liver abscesses in harvested cattle , the method comprising the steps of:a) feeding an animal feed composition to cattle, wherein the animal feed composition comprises plant material from a transgenic plant or plant part expressing a recombinant thermotolerant microbial α-amylase; andb) harvesting the cattle.2. The method of claim 1 , wherein the microbial α-amylase comprises a polypeptide having at least 80% identity to the amino acid sequence of SEQ ID NO:1 or a polypeptide encoded by a nucleotide sequence having at least 80% identity to the nucleotide sequence of SEQ ID NO:2 claim 1 , SEQ ID NO:3 claim 1 , SEQ ID NO:4 and/or SEQ ID NO:5.3. The method of claim 1 , wherein the transgenic plant or plant part comprises about 1% to about 100% by weight of the plant material.4. The method of claim 1 , wherein the plant material comprises from about 5% to about 100% by weight of the animal feed composition.5. The method of claim 1 , wherein the plant material is corn.6. The method of claim 5 , wherein the corn comprises corn event 3272.7. The method of claim 5 , wherein the corn is steam flaked.8. The method of claim 1 , wherein the cattle are beef cattle.9. The method of claim 1 , wherein the ...

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Номер записи: 62
12-03-2020 дата публикации

GLYCOSYLATED OXALATE DECARBOXYLASE AND PREPARATION AND APPLICATION THEREOF

Номер: US20200080070A1
Автор: CHEN Huoqing, CHEN Xianqiao, Liu Haifeng, SONG Baoping, TONG Pei, Wang Wei, Wang Xiaofeng
Принадлежит: Wuhan Kangfude Biotechnology Co., Ltd.

The present invention discloses a glycosylated oxalate decarboxylase. The oxalate decarboxylase is derived from edible basidiomycetes, the glycosylated oxalate decarboxylase has an enzyme activity at the pH value of 1.5-7.5, the activity at the pH value of 1.5-2.0 is 10% greater than the optimum activity, and a specific activity is more than 2 U/mg. The present invention also discloses preparation and application of the glycosylated oxalate decarboxylase. The glycosylated oxalate decarboxylase disclosed by the present invention can keep the activity under a low pH value, and can effectively prevent and treat kidney stones. 1. A glycosylated oxalate decarboxylase , characterized in that the oxalate decarboxylase is derived from edible basidiomycetes , the glycosylated oxalate decarboxylase has an enzyme activity at the pH value of 1.5-7.5 , the activity at the pH value of 1.5-2.0 is greater than 10% of an optimum activity , and a specific activity is more than 2 U/mg.2. The glycosylated oxalate decarboxylase of claim 1 , characterized in that the glycosylated oxalate decarboxylase has an enzyme activity at the pH value of 2.0-5.5 claim 1 , the activity is more than 30% of the optimal activity claim 1 , and the specific activity is more than 6 U/mg.3. The glycosylated oxalate decarboxylase of claim 1 , characterized in that the optimal pH value of the enzyme activity of the oxalate decarboxylase is pH 2.5-3.0.4. The glycosylated oxalate decarboxylase of claim 3 , characterized in that when the oxalate decarboxylase is at the optimal pH value claim 3 , the activity unit is 20-200 U/mg claim 3 , and the Kin value is 0.06 mM.5Agrocybe.. The glycosylated oxalate decarboxylase of one of - claim 3 , characterized in that the oxalate decarboxylase comes from edible fungi of6Agrocybe aegerita, Agrocybe cylindracea, Agrocybe praecoxfayod, Agrocybe salicacolaAgrocybe pediadesFayod.. The glycosylated oxalate decarboxylase of claim 5 , characterized in that the oxalate ...

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Номер записи: 63
30-03-2017 дата публикации

PROTEIN HYDROLYSATES AS AGENTS TO TREAT SYMPTOMS OF ADDICTION

Номер: US20170087226A1
Автор: FERNANDEZ-REUMANN Guillen Santiago Eusebio, Goralczyk Regina, Kloek Joris, KROES Marijn Christoffel Waander, Mohajeri Hasan, Van Wingen Guido Alexander, Wittwer Jonas
Принадлежит:

This invention is directed to the use of a protein hydrolysate, and in particular an egg lysozyme hydrolysate to assist an animal, including a human in overcoming an addition, or by breaking an unwanted habit. 1. A method of assisting an animal in refraining from an undesired behavior , and/or resisting from indulging in an addictive behavior comprising: a) administering a hydrolyzed hen egg lysozyme composition which provides a daily dose of up to 800 mg tryptophan , said composition being characterized by a Trp/LNAA ratio between 0.15 and 0.20 , and said composition not containing free Trp , and b) noticing abstinence from the behavior.2. The method according to wherein the animal is a human.3. The method according to wherein the animal is an animal in a zoo claim 1 , a farm animal claim 1 , a pet or companion animal claim 1 , or a racing animal.4. The method according to wherein the behavior is selected from the group consisting of: smoking claim 1 , alcohol abuse claim 1 , gambling claim 1 , drug abuse claim 1 , compulsive behaviors claim 1 , uncontrollable behaviors claim 1 , cravings claim 1 , and potentially harmful risky behaviors.5. The method according to wherein the behavior is a habit which the individual wishes to break.6. The method according to wherein the hydro lyzed lysozyme composition is present in a food claim 1 , feed claim 1 , nutraceutical claim 1 , or food supplement.7. The method according to wherein the hydrolyzed lysozyme is an adjunctive treatment or supportive accompanying diet during dehabituation programs claim 1 , or to assist dehabituation processes.8. A method of doing business comprising marketing a hydrolyzed hen egg lysozyme composition which provides a daily dose of up to 800 mg tryptophan claim 1 , said composition being characterized by a Trp/LNAA ratio between 0.15 and 0.20 claim 1 , and said composition not containing free Trp claim 1 , for sale to a consumer comprising: informing the consumer that the hydrolyzed lysozyme ...

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Номер записи: 64
30-03-2017 дата публикации

NUTRIENT RICH COMPOSITIONS

Номер: US20170088477A1
Автор: LeJeune Mark, Morash Daniel M.
Принадлежит:

This invention relates to processes and systems for converting fresh food waste into nutrient rich hydrolysates and particulate compositions. The invention also relates to the hydrolysates and compositions useful, for example, as fertilizers, feedstock or other nutrient supplements. 1. (canceled)2. A nutrient rich emulsified hydrolysate made from fresh food waste , comprising nutrients released by grinding , shearing , homogenization and enzymatic digestion by a combination of enzymes comprising at least one enzyme to digest proteins , at least one enzyme to digest fats and lipids , at least one enzyme to digest cellulosic material and at least one enzyme to digest other carbohydrates; and a species selected from an acid stabilizer or an organic preservative , wherein the emulsified hydrolysate has an average particle size of less than about 30 μm and a pH of between about 2.5 and 3.5.3. The nutrient rich emulsified hydrolysate of wherein multiple batches of the hydrolysate have been blended in large storage tanks with circulation pumps claim 2 , to assure the consistency of the finished product.4. (canceled)5. (canceled)6. The emulsified hydrolysate of claim 2 , wherein the organic preservative added in step (f) is selected from: tocopherol claim 2 , D-α tocopherol acetate claim 2 , or natamycin.7. The emulsified hydrolysate of claim 6 , wherein the organic preservative is tocopherol or D-α tocopherol acetate at a level of 10 to 150 mg/kg.8. The emulsified hydrolysate of claim 6 , wherein the organic preservative is natamycin at a level of 0.1 to 100 mg/ml.913.-. (canceled)14. The emulsified hydrolysate of claim 2 , wherein the pH of the stabilized liquid hydrolysate is about 3.0. The present application is a Continuation application of U.S. patent application Ser. No. 15/045,041, filed on Feb. 16, 2016, which is a Divisional application of U.S. patent application Ser. No. 14/869,737, filed on Sep. 29, 2015 and Issued as U.S. Pat. No. 9,416,062 on Aug. 16, 2016, ...

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Номер записи: 65
05-05-2022 дата публикации

FEED ADDITIVE FORMULATION AND METHODS OF MAKING AND USING THE SAME

Номер: US20220133816A1
Автор: SHIH Giles, Wang Jeng-Jie
Принадлежит:

The presently disclosed subject matter relates to feed additive formulations for monogastric animal feed. Particularly, the disclosed formulations comprise an isolated xylanase enzyme and a strain PWD-1. The feed additive formulations may further include strain Ba-BPD1. The disclosed formulations are useful for addition to feeds for monogastric animals to synergistically improve the performance of the animals.

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Номер записи: 66
12-05-2022 дата публикации

FEED FOR ENHANCING IMMUNITY OF TILAPIA AND PREPARATION METHOD THEREOF

Номер: US20220142205A1
Автор: Cai Shuanghu, Huang Yu, Jian Jichang, Wang Bei, Wang Zhongliang
Принадлежит:

A feed for enhancing immunity of tilapia and preparation method thereof, wherein the feed includes 20-30 parts of rice chaff, 15-25 parts of earthworm powder, 10-15 parts of shrimp shell powder, 10-15 parts of sodium alginate, 5-10 parts of oyster shell powder, 5-10 parts of cyclohexanone, 10-15 parts of honeysuckle extract, 3-5 parts of extract, 3-6 parts of β-1,3-glucan, 2-3 parts of flavomycin growth promoter, 1-3 parts of complex enzyme preparation, 5-8 parts of selenium methionine, 6-10 parts of distiller's grains, 1-3 parts of complex vitamins, 1-3 parts of complex trace elements and 2-3 parts of food attractant, in parts by weight. 1Commelina communis. A feed for enhancing immunity of tilapia , wherein the feed comprises 20-30 parts of rice chaff , 15-25 parts of earthworm powder , 10-15 parts of shrimp shell powder , 10-15 parts of sodium alginate , 5-10 parts of oyster shell powder , 5-10 parts of cyclohexanone , 10-15 parts of honeysuckle extract , 3-5 parts of extract , 3-6 parts of β-1 ,3-glucan , 2-3 parts of flavomycin growth promoter , 1-3 parts of complex enzyme preparation , 5-8 parts of selenium methionine , 6-10 parts of distiller's grains , 1-3 parts of complex vitamins , 1-3 parts of complex trace elements and 2-3 parts of food attractant , in parts by weight.2. The feed according to claim 1 , wherein the complex enzyme preparation is a mixture of amylase claim 1 , protease and cellulase claim 1 , and the weight ratio of amylase claim 1 , protease and cellulase is 3:2:1.3. The feed according to claim 1 , wherein the complex vitamins comprises two or more of vitamin C claim 1 , vitamin E claim 1 , vitamin K claim 1 , vitamin A claim 1 , vitamin D3 claim 1 , nicotinic acid claim 1 , folic acid claim 1 , inositol claim 1 , and calcium pantothenate.4. The feed according to claim 1 , wherein the complex trace elements comprise iron claim 1 , copper claim 1 , manganese and iodine.5. The feed according to claim 1 , wherein the food attractant comprises ...

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Номер записи: 67
19-03-2020 дата публикации

CELL-ASSOCIATED HETEROLOGOUS FOOD AND/OR FEED ENZYMES

Номер: US20200087672A1
Автор: Argyros Aaron, Fisher Janet, Henningsen Brooks, Kraus J. Kevin, Oeser Michelle, Skinner Ryan, Van Eijk Johannes, Wenger Kevin, Wiswall Erin
Принадлежит:

The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products. 1. A recombinant yeast host cell having an heterologous nucleic acid molecule encoding a cell-associated heterologous food and/or feed enzyme , wherein the heterologous nucleic acid molecule is operatively associated with an heterologous promoter allowing the expression of the heterologous nucleic acid molecule during propagation.2. The recombinant yeast host cell of claim 1 , wherein the heterologous nucleic acid molecule allows the intracellular expression of the heterologous food and/or feed enzyme.3. The recombinant yeast host cell of claim 1 , wherein the heterologous nucleic acid molecule allows the expression of a membrane-associated heterologous food and/or feed enzyme.4. The recombinant yeast host cell of claim 3 , wherein the heterologous nucleic acid molecule allows the expression of a tethered heterologous food and/or feed enzyme.5. The recombinant yeast host cell of claim 4 , wherein the tethered heterologous food and/or feed enzyme is a chimeric protein of formula (I) or (II):{'br': None, 'sub': '2', '(NH)FFE-L-TT(COOH)\u2003\u2003(I)'}{'br': None, 'sub': '2', '(NH)TT-L-FFE(COOH)\u2003\u2003(II)'} L is present or absent and is an amino acid linker;', 'TT is an amino acid tethering moiety for associating the food and/or feed enzyme to a cell wall of the recombinant yeast host cell;', {'sub': '2', '(NH) indicates the amino terminus of the chimeric protein;'}, '(COOH) indicates the carboxyl terminus of the chimeric protein; and', '“-” is an amide linkage., 'wherein FFE is the food and/or feed enzyme;'}6. The recombinant yeast host cell of any one of to claim 4 , wherein the heterologous nucleic acid ...

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Номер записи: 68
05-04-2018 дата публикации

STABLE GRANULES WITH LOW INTERNAL WATER ACTIVITY

Номер: US20180092379A1
Автор: BECKER Nathaniel T., WHITE Luther
Принадлежит: DANISCO US INC.

Described are stable, durable granules with active agents, which have consistently low internal water activity, and methods for making such granules. The granules are particularly suitable for inclusion in steam treatment processes, including pelleting and tableting processes and steam processing of feed, without appreciable loss of active agent activity. The granules can be manufactured using humid processing air without compromising the stability of the active agents 1. A granule for delivering an active agent comprising:a core;an active agent; andat least one coating, the active agent of the granule retaining at least 50% activity, at least 60% activity, at least 70% activity, at least 80% activity after conditions selected from one or more of a) a feed pelleting process, b) a steam-heated feed pretreatment process, c) storage, d) storage as an ingredient in an unpelleted mixture, and e) storage as an ingredient in a feed base mix or a feed premix comprising at least one compound selected from trace minerals, organic acids, reducing sugars, vitamins, choline chloride, and compounds which result in an acidic or a basic feed base mix or feed premix;wherein the granule has an internal water activity less than about 0.40, less than about 0.35 or less than about 0.30.2. The granule of claim 1 , wherein the at least one coating comprises a moisture hydrating material that constitutes at least 55% w/w of the granule.3. The granule of or claim 1 , wherein the at least one coating comprises two coatings.4. The granule of claim 3 , wherein the two coatings are a moisture hydrating coating and a moisture barrier coating.5. The granule of claim 4 , wherein the moisture hydrating coating is between 25% and 60% w/w of the granule and the moisture barrier coating is between 2% and 15% w/w of the granule.6. The granule of or claim 4 , wherein the moisture hydrating coating is selected from inorganic salts claim 4 , sucrose claim 4 , starch claim 4 , and maltodextrin and the ...

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Номер записи: 69
28-03-2019 дата публикации

XYLANASES FOR SOLUBILIZING ARABINOXYLAN-CONTAINING MATERIAL

Номер: US20190090525A1
Автор: DALSGAARD SOEREN, KIARIE ELIJAH G., LAU ROSALYN, LAURSEN BRIAN SOEGAARD, LUND SUSAN ARENT, PEDERSEN MADS BROEGGER, ROMERO MILLAN LUIS FERNANDO, Yu Zheyong, Zhang Zhenghong
Принадлежит:

The present invention relates to a method of preparing a corn based product said method comprising contacting a plant composition comprising (consisting of or consisting essentially of) corn or a corn by-product or a combination thereof with a xylanase comprising: i) a polypeptide as set forth in SEQ ID No. 8 or SEQ ID No. 7 or SEQ ID No. 6; or ii) a variant, fragment homologue, fragment or derivative thereof having at least 85% identity with SEQ ID No. 8 or SEQ ID No. 7 or SEQ ID No. 6; or with a xylanase encoded by: a) a nucleotide sequence shown herein as SEQ ID No. 3. SEQ ID No. 2 or SEQ ID No. 1; or b) a nucleotide sequence which can hybridize to the complement of SEQ ID No. 3. SEQ ID No. 2 or SEQ ID No. 1 under high stringency conditions: or c) a nucleotide sequence which has at least 80% identity with SEQ ID No. 3. SEQ ID No. 2 or SEQ ID No. 1. The present invention further relates to the use of the enzyme to produce corn based feedstuffs. The present invention yet further relates to a method of producing a fermented beverage comprising the step of contacting a mash and/or a wort with a xylanase comprising a polypeptide as set forth in SEQ ID No. 8 or SEQ ID No. 7 or SEQ ID No. 6; or a variant, fragment, homologue or derivative thereof having at least 85% identity with SEQ ID No. 8 or SEQ ID No. 7 or SEQ ID No. 6; or encoded by a nucleotide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1, or a nucleotide sequence which can hybridize to the complement of SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1 under high stringency conditions, or a nucleotide sequence which has at least 80% identity with SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1. 1. A method of preparing a corn based product comprising contacting a plant composition comprising corn or a corn by-product or a combination thereof with:(a) a xylanase comprising a polypeptide as set forth in SEQ ID No. 8 or SEQ ID No. 7 or SEQ ID No. 6; or a variant, homologue or derivative thereof having ...

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Номер записи: 70
26-03-2020 дата публикации

BREEDING WATER FOR ANGUILLIFORMES AND METHOD FOR REARING ANGUILLIFORMES

Номер: US20200093103A1
Автор: Kawakami Yutaka, Nagata Ryoichi
Принадлежит: SHIN NIPPON BIOMEDICAL LABORATORIES, LTD.

Anguilliformes breeding water contains a thyroid hormone such as thyroxine, and a method for rearing anguilliformes includes a step for administering a thyroid hormone such as thyroxine, wherein the amount of the thyroid hormone to be administered changes as the anguilliforme fingerling changes from a transformation start phase (stage 1) to a transformation final phase (stage 2), and the amount of the thyroid hormone to be administered in the transformation final phase (stage 2) is less than the amount of the thyroid hormone to be administered in the transformation start phase (stage 1). 1. Breeding water for Anguilliformes , comprising a thyroid hormone.2. The breeding water for Anguilliformes according to claim 1 , wherein the thyroid hormone contains thyroxine.3. The breeding water for Anguilliformes according to claim 1 , further containing a vitamin.4. The breeding water for Anguilliformes according to claim 1 , which is breeding water for larvae of Anguilliformes.5. A method for rearing Anguilliformes claim 1 , comprising a step of administering a thyroid hormone.6. The method according to claim 5 , whereina dose of the thyroid hormone is changed as larvae of Anguilliformes shift from a starting phase of metamorphosis (stage 1) to a peak phase of metamorphosis (stage 2), andthe dose of the thyroid hormone in the peak phase of metamorphosis (stage 2) is lower than the dose of the thyroid hormone in the starting phase of metamorphosis (stage 1).7. The method according to claim 6 , whereinthe dose of the thyroid hormone is changed as larvae of Anguilliformes shift from the peak phase of metamorphosis (stage 2) to a late phase of metamorphosis (stage 3), andthe dose of the thyroid hormone in the late phase of metamorphosis (stage 3) is lower than the dose of the thyroid hormone in the peak phase of metamorphosis (stage 2).8. The method according to claim 7 , whereinwhen the dose of the thyroid hormone in the starting phase of metamorphosis (stage 1) is expressed ...

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Номер записи: 71
04-04-2019 дата публикации

PHYTASE

Номер: US20190100734A1
Автор: JR. Arne I., Solbak
Принадлежит:

Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/l to 40 g/L. The phytases have higher specific activity, retain activity at low pH, retain activity at high temperature, increased phosphorous equivalency, increased phosphorous bioavailability, and increased phosphorous hydrolysis. The phytases can be used in a variety of industries including food, feed, ethanol production, pharmaceuticals, and cleaning. 1E. coliE. coli. A phytase having greater phosphorous equivalency , greater phosphorous bioavailability , or greater phosphorous hydrolysis as compared to a fungal , , or a modified phytase.2. The phytase of claim 1 , wherein the phytase has a phosphorous equivalency in a range from 0.11 to 0.23%.3E. coli. The phytase of claim 1 , wherein the phytase has at least 60 to 65% more phosphorous bioavailability when compared to a fungal or phytase.4E. coli. The phytase of claim 1 , wherein the phytase has at least 20 to 30% more phosphorous bioavailability when compared to a modified phytase.5. The phytase of claim 1 , wherein the phytase hydrolyzes up to 90% of phosphorous from a feed comprising phytic acid.6. The phytase of claim 1 , wherein the phytase has at least 100% activity for more than 5 minutes at low pH.7. The phytase of claim 1 , wherein the phytase has improved activity at phytic acid concentrations from 0.2 mM to 5.1 mM of phytic acid.8. The phytase of claim 1 , wherein the phytase has a melting temperature greater than about 90 degrees C.9. A phytase having at least 90% activity at 2.5 minutes.10. A phytase that hydrolyzes anti-nutritive phytase IP2 claim 1 , IP3 claim 1 , IP4 claim 1 , IP5 claim 1 , and IP6 from a range of about 91% to about 98%.11. The phytase of claim 1 , wherein the phytase has at least 85% sequence identity to SEQ ID NO:3 claim 1 , SEQ ID NO: 6 claim 1 , or SEQ ID NO: 8.12. The phytase of claim 11 , wherein the phytase has at ...

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Номер записи: 72
29-04-2021 дата публикации

METHOD FOR PRODUCING A PROTEIN HYDROLYSATE EMPLOYING AN ASPERGILLUS FUMIGATUS TRIPEPTIDYL PEPTIDASE

Номер: US20210120845A1
Автор: Bak Steffan Yde, Degn Peter Edvard, EISELE Thomas, Haaning Svend, Kragh Karsten Matthias, MEINJOHANNS Ernst
Принадлежит:

The present invention relates to compositions and methods for the production of a hydrolysate comprising at least one endoprotease and a tripeptidyl peptidase capable of cleaving tripeptides from the N-terminus a peptide and/or proteins having one or more of lysine, arginine or glycine in the P1 position wherein said tripeptidyl peptidase is capable of being used at a temperature between 45° C. and 70° C. 1. A method for the production of a hydrolysate comprising: i) comprises the amino acid sequence SEQ ID No. 3, SEQ ID No. 4 or a functional fragment thereof;', 'ii) comprises an amino acid having at least 70% identity to SEQ ID No. 3 or SEQ ID No. 4;', 'iii) is encoded by a nucleotide sequence comprising the sequence SEQ ID No. 1 or SEQ ID No. 2;', 'iv) is encoded by a nucleotide sequence which has at least about 70% identity to SEQ ID No. 1 or SEQ ID No. 2;', 'v) is encoded by a nucleotide sequence which hybridises to SEQ ID No. 1 or SEQ ID No. 2 under medium stringency conditions; or', 'vi) is encoded by a nucleotide sequence which differs from SEQ ID No. 1 or SEQ ID No. 2 due to degeneracy of the genetic code;, 'a) admixing at least one protein or a portion thereof with a tripeptidyl peptidase whichb) incubating at a temperature between 45° C. and 70° C., andc) recovering the hydrolysate.2. A method according to wherein the temperature of the incubation is between 50° C. and 65° C.3. A method according to wherein the temperature of the incubation is between 55° C. and 65° C.4. A method according to claim 3 , wherein the method further comprises admixing the recovered hydrolysate with at least one feed or food ingredient.5. A method according to wherein the protein or portion thereof is further treated with an endoprotease.6. A method according to wherein the endoprotease and the tripeptidyl peptidase are added simultaneously.7. A method according to wherein the endoprotease and the tripeptidyl peptidase are added sequentially claim 6 , e.g. with the tripeptidyl ...

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Номер записи: 73
20-04-2017 дата публикации

MUTANT XYLANASE, MANUFACTURING METHOD AND USE THEREFOR, AND METHOD FOR MANUFACTURING SACCHARIFIED LIGNOCELLULOSE

Номер: US20170107502A1
Автор: INOUE Atsushi, OKAKURA Kaoru, OSABE Masami, Tamai Hiroki, YANAI Hisaaki, Yokoyama Fumikazu
Принадлежит:

What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50° C. for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue. 1. A mutant xylanase comprising:(a) an amino acid sequence including the following amino acid residue substitution in the amino acid sequence of SEQ ID NO: 2:a methionine residue substituted for a leucine residue at position 154, or(b) an amino acid sequence including 1 to 10 amino acid residue substitutions in the amino acid sequence of SEQ ID NO: 2 in addition to a methionine residue being substituted for a leucine residue at position 154.2. The mutant xylanase according to claim 1 , wherein the mutant xylanase comprises:(a) an amino acid sequence including the following amino acid residue substitutions (i), (ii) or (iii) in the amino acid sequence of SEQ ID NO: 2: an arginine residue substituted for a glycine residue at position 36;', 'a serine residue substituted for a threonine residue at position 90;', 'an arginine residue substituted for a glutamine residue at position 132;', 'a methionine residue substituted for a leucine residue at position 154;', 'a threonine residue substituted for a serine residue at position 174;', ...

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Номер записи: 74
02-04-2020 дата публикации

ENZYME-INHIBITOR COMPLEXES

Номер: US20200102548A1
Автор: Arnaut Filip, BOSMANS Geertrui, BRIJS Kristof, Delcour Jan, DORGEO Valérie, Fierens Ellen, GEORIS Jacques, VERTE Fabienne
Принадлежит: PURATOS NV

The present invention concerns a method for selectively activating a thermostable hydrolase at a temperature above T1. The present invention provides compositions comprising a thermostable hydrolase and a temperature sensitive inhibitor, wherein said thermostable hydrolase and said temperature sensitive inhibitor form a hydrolase-inhibitor complex at a temperature below T1, but which dissociates at a temperature of about T1. The present invention also relates to uses of said compositions, and a method for preparing said compositions. 19-. (canceled)10. A composition comprising a thermostable hydrolase and a temperature sensitive inhibitor , wherein said thermostable hydrolase and said temperature sensitive inhibitor form a hydrolase-inhibitor complex at a temperature below T1 , wherein said inhibitor inhibits the activity of said hydrolase in said complex at a temperature below T1 and dissociates from said complex at a temperature of about T1.11Thermus aquaticus. The composition according to claim 10 , wherein the hydrolase is a protease claim 10 , preferably a serine protease claim 10 , more preferably Aqualysin I from LMG 8924.12. The composition according to claim 10 , wherein the inhibitor is of vegetable origin or a recombinant claim 10 , semi-synthetic claim 10 , or synthetic variant thereof.13. The composition according to claim 10 , wherein the inhibitor is a trypsin inhibitor claim 10 , preferably soybean trypsin inhibitor.14. The composition according to claim 10 , wherein said temperature T1 is at least 40° C. claim 10 , preferably at least 50° C. claim 10 , more preferably at least 60° C.15. The composition according to claim 10 , wherein said inhibitor is irreversibly inactivated at a temperature of about T1.16. The composition according to claim 10 , wherein said hydrolase has maximal activity at a temperature above T1.17. The composition according to claim 10 , wherein the activity of the enzyme in the complex is less than 25% of the activity of the ...

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Номер записи: 75
09-06-2022 дата публикации

Anti-Aging Therapy for Canines and Other Domesticated Animals

Номер: US20220175694A1
Автор: Richard Postrel
Принадлежит: Individual

The present invention discloses a system and method for improving the quality of life in canines and other domesticated animals. As animals age testosterone and other supportive hormones diminish causing reduced activity, a lack of mobility, decreased bone density, and general well-being. Precursor compositions are selected and designed for the purpose of formulating non-toxic therapeutic hormone supplements for oral consumption. The process and method of this invention is comprised of therapies produced from two or more selected non-toxic prohormones that combine during digestion and delivered through a chewable and edible gummy treat. As such there is no requirement for changing the subject's current diet. The present invention features a continuous low dose supplementation to be incorporated daily whereby dosages are correlated according to the subject's age, weight, sex, and breed. The beneficial results of the present invention are achieved through the absorption of the prohormones in the composition into the bloodstream effecting delivery to all cells including those specific cells where the conversion enzymes are activated by the organism's needs. The delivery and transport of inactive prohormones minimizes liver toxicity since the precursors are carried through the hepatic portal system to and through the liver and into general circulation in inactive forms.

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Номер записи: 76
13-05-2021 дата публикации

NUCLEASES AND METHODS FOR MAKING AND USING THEM

Номер: US20210139871A1
Автор: BARRETT Kenneth, Dennis Jared, Dodge Corey, REN Dongmei, Tan Xuqiu, Zhang Bin
Принадлежит:

Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits. 160-. (canceled)61. A synthetic or recombinant polypeptide comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 1 , wherein the polypeptide has nuclease activity , and wherein the polypeptide comprises one or more mutations of E230L , L114F , E260R , E230M , D246P , S161R , N54S , T227H , E230R , G263A , G119N , V226K , N127S , P84V , D83E , D28G , V45T , M262V , A190K , P84N , I44R , G256S , A73M , P179L , Q135E , A60P , V247I , G263K , S161E , P72N , P84L , S74N , T82R , G75R , Q141R , and D107N.62. The polypeptide of claim 61 , wherein the polypeptide comprises a mutation or a combination of mutations selected from the group consisting of(T82R, L114F);(T82R, L114F, G119N);(T82R)(T82R, G119N);(L114F);(L114F, G119N);(G119N);(G75R, T82R, L114F, G119N);(G75R, T82R, L114F, G119N);(G75R, T82R);(G75R, L114F);(G75R, L114F, G119N);(S74N, G75R, T82R, G119N, Q141R);(S74N, T82R, L114F, G119N);(S74N, T82R, L114F, G119N, Q141R);(S74N, T82R, Q141R);(S74N, T82R, G119N, Q141R);(S74N, L114F, Q141R);(S74N, L114F, G119N, Q141R);(S74N, Q141R);(S74N, G75R, T82R, L114F, Q141R);(S74N, G75R, T82R, L114F, G119N, Q141R);(S74N, G75R, T82R, Q141R);(S74N, G75R, L114F, Q141R);(S74N, G75R, Q141R);(T82R, D107N);(G75R, T82R, D107N);(D107N);(G75R, T82R, G119N);(T82R, D107N, G119N);(G75R, T82R, D107N, G119N);(D107N, G119N);(G75R, D107N, G119N);(S74N, T82R, D107N, Q141R);(S74N, G75R, T82R, D107N, Q141R);(S74N, G75R, D107N, Q141R);(S74N, G119N, Q141R);(S74N, G75R, G119N, Q141R);(S74N, T82R, D107N, G119N, Q141R);(S74N, G75R, T82R, D107N, G119N, Q141R);(S74N, ...

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Номер записи: 77
03-05-2018 дата публикации

GRANULATED FEED PHOSPATE COMPOSITION INCLUDING FEED ENZYMES

Номер: US20180116248A1
Автор: Baylor Bryan, Brittain Charlotte, Fontana Eddy A., Jones Everett L., Marsella Reinaldo M., Nsheiwat Sal, Thomas Ryan
Принадлежит:

A granulated phosphate composition that provides sufficient nutritional value as well as enhanced bioavailability of organic phosphorous present in a main feed ingredient. The granulated phosphate composition includes a phosphate source, such as monocalcium phosphate, mono-dicalcium phosphate, or dicalcium phosphate, and one or more feed enzymes such as phytase. When the phosphate composition is compounded and pelleted with a main feed ingredient into a feed pellet, and consumed by a non-ruminant, the phytase component of the phosphate composition liberates otherwise unavailable phosphorous present in the main feed ingredient. 1. A granulated phosphate composition to be used as an ingredient in animal feed , the phosphate composition comprising:a phosphate source; anda feed enzyme.2. The granulated phosphate composition of claim 1 , wherein the phosphate source comprises monocalcium phosphate claim 1 , mono-dicalcium phosphate claim 1 , dicalcium phosphate claim 1 , or combinations thereof.3. The granulated phosphate composition of claim 1 , wherein the feed enzyme is selected from the group consisting of phytases claim 1 , amylases claim 1 , xylanases claim 1 , proteases claim 1 , and combinations thereof4. The granulated phosphate composition of claim 3 , wherein the feed enzyme comprises a phytase.5. The granulated phosphate composition of claim 4 , wherein the phytase is present in an amount such that the phytase provides from about 200 to about 2 claim 4 ,000 FTU per kilogram of animal feed.6. The granulated phosphate composition of claim 3 , further comprising one or more additional feed enzymes selected from the group consisting of amylases claim 3 , xylanases claim 3 , proteases claim 3 , and combinations thereof.7. The granulated phosphate composition of claim 6 , wherein the one or more additional feed enzymes are present in an amount to provide from about 50 to about 500 grams per ton of animal feed.8. The granulated phosphate composition of claim 1 , ...

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Номер записи: 78
04-05-2017 дата публикации

COMPOSITIONS COMPRISING DEFATTED MICROALGAE, AND TREATMENT METHODS

Номер: US20170119018A1
Автор: Lei Xingen
Принадлежит:

The present invention relates to a composition comprising defatted microalgae in a solid, powder, or liquid form formulated for oral administration. The present invention also relates to a composition comprising defatted microalgae formulated as a food additive. Also disclosed are treatment methods that involve administering microalgae compositions. 1. A composition comprising defatted microalgae (i) in a solid , powder , or liquid form formulated for oral administration or (ii) formulated as a food additive.2. (canceled)3. The composition of claim 1 , wherein the defatted microalgae comprises green marine microalgae.4Nannochloropsis oceanica.. The composition of claim 3 , wherein the defatted microalgae comprises5. The composition of claim 1 , wherein the defatted microalgae comprises 0.1%-50% of oil content compared to non-defatted microalgae.6. The composition of claim 1 , wherein the defatted microalgae comprises iron at a concentration of at least about 100 ppm.7. The composition of claim 6 , wherein at least about 10% of the iron is high availability iron.8. The composition of further comprising:phytase.9. A method of treating iron deficiency in an iron deficient animal claim 1 , said method comprising:identifying an iron deficient animal andadministering to the iron deficient animal a composition comprising microalgae (i) in a solid, powder, or liquid form formulated for oral administration or (ii) formulated as a food additive under conditions effective to treat the iron deficient animal.10. (canceled)11. The method of claim 9 , wherein the animal is a human.12. The method of claim 9 , wherein the animal is selected from the group consisting of a ruminant claim 9 , poultry claim 9 , swine aquaculture claim 9 , pet claim 9 , dog claim 9 , cat claim 9 , horse claim 9 , zoo animal claim 9 , mouse claim 9 , rat claim 9 , rabbit claim 9 , guinea pig claim 9 , and hamster.13. The method of claim 9 , wherein the microalgae comprises defatted microalgae.14. The ...

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Номер записи: 79
25-08-2022 дата публикации

FEED COMPOSITION

Номер: US20220264911A1
Автор: Horsmans Poulsen Charlotte, Kragh Karsten Matthias, Li Wenting, MARCHAL Leon, Tse Kathryn Louise, VILJANEN Jouni, Yu Shukun
Принадлежит:

Provided herein, inter alia, are granules for inclusion in feed or feed additive compositions as well as methods for making and using the same for improving the performance of a ruminant animal with respect to one or more of improved feed conversion ratio (FCR), improved weight gain, improved feed efficiency, improved carcass quality, and/or improved milk production. 1. A granule comprising (a) a core comprising (i) an enzyme; and (ii) a carrier comprising at least one proton acceptor; and (b) one or more layers of one or more fats , wherein the core is coated by the one or more fats and wherein the enzyme maintains at least about 50% residual activity after being coated.2. The granule of claim 1 , wherein the granule is from about 100 μm to about 1500 μm diameter in size.3. The granule of or claim 1 , wherein the granule has a particle density from about 0.6 g/mL to about 1.2 g/mL.4. The granule of any one of - claim 1 , wherein the enzyme maintains at least about 55% claim 1 , about 60% claim 1 , about 65% claim 1 , about 70% claim 1 , about 75% claim 1 , about 80% claim 1 , about 85% claim 1 , about 90% claim 1 , about 95% claim 1 , or about 100% residual activity after being coated.5. The granule of any one of - claim 1 , wherein the composition has a moisture content of about 5% (w/w) or less.6. The granule of any one of - claim 1 , wherein the coating is a fat is selected from the group consisting of animal oils or fats claim 1 , vegetable oils or fats claim 1 , triglycerides claim 1 , free fatty acids claim 1 , animal waxes claim 1 , beeswax claim 1 , lanolin claim 1 , shell wax claim 1 , Chinese insect wax claim 1 , vegetable waxes claim 1 , carnauba wax claim 1 , candelilla wax claim 1 , bayberry wax claim 1 , sugarcane wax claim 1 , mineral waxes claim 1 , synthetic waxes claim 1 , natural and synthetic resins claim 1 , and mixtures thereof.7. The granule of claim 6 , wherein the fat is an animal fat or oil and/or a plant fat or oil.8. The granule of claim ...

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Номер записи: 80
25-04-2019 дата публикации

FEED ADDITIVE COMPOSITION

Номер: US20190117706A1
Автор: Bernardeau Marion, Davis Mari Ellen, Forssten Sofia, Galbraith Elizabeth Ann, ISAKSEN MAI FAURSCHOU, KIARIE ELIJAH G., LUND SUSAN ARENT, NURMINEN PÄIVI, Petri Daniel, ROMERO MLLAN LUIS FERNANDO
Принадлежит:

A feed additive composition comprising a direct fed microbial (DFM), in combination with a xylanase (e.g. endo-1,4-β-d-xylanase) and a β-glucanase (and optionally a further fibre degrading enzyme), wherein the DFM is selected from the group consisting of an enzyme producing strain; a C5 sugar-fermenting strain; a short-chain fatty acid-producing strain; a fibrolytic, endogenous microflora-promoting strain; or combinations thereof. The DFM may be selected from the group consisting of: AGTP BS3BP5, AGTP BS442, AGTP BS521, AGTP BS918, AGTP BS1013, AGTP BS1069, AGTP 944, AGTP BS 1068 or KX11-1, ID7, P169, CNCM-I-3698, CNCM-I-3699, a strain having all the characteristics thereof, any derivative or variant thereof, and combinations thereof and the further fibre degrading enzyme may be selected from the group consisting of a cellobiohydrolase (E.C. 3.2.1.176 and E.C. 3.2.1.91), a β-glucosidase (E.C. 3.2.1.21), a β-xylosidase (E.C. 3.2.1.37), a feruloyl esterase (E.C. 3.1.1.73), an α-arabinofuranosidase (E.C. 3.2.1.55), a pectinase (e.g. an endopolygalacturonase (E.C. 3.2.1.15), an exopolygalacturonase (E.C. 3.2.1.67) or a pectate lyase (E.C. 4.2.2.2)), or combinations thereof. 1. (canceled)2. A method for improving the performance of a subject or for improving digestibility of a raw material in a feed , or for improving nitrogen retention , or for improving feed conversion ratio (FCR) , or for improving weight gain in a subject , or for improving feed efficiency in a subject , or for shifting the fermentation process in the subject's gastrointestinal tract towards the production of butyric acid and/or propionic acid , the method comprising administering a direct fed microbial (DFM) to the subject , in combination with a xylanase and a β-glucanase , wherein the DFM is selected from the group consisting of an enzyme producing strain; a C5 sugar-fermenting strain; a short-chain fatty acid-producing strain; a fibrolytic , endogenous microflora-promoting strain; and ...

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Номер записи: 81
16-04-2020 дата публикации

METHOD FOR CONCENTRATING PROTEIN IN GRAIN POWDER

Номер: US20200113208A1
Автор: CHO Seong Jun, Han Sung Wook, Hong Young Ho, KANG Kyeong Il, Park Seung Won, RYU Je Hoon, SEO Hyo Jeong
Принадлежит: CJ CHEILJEDANG CORPORATION

Provided are a method of concentrating protein in grain powder, grain powder including protein that has been concentrated by using the method, and a feed additive including the grain powder including concentrated protein. According to the method of concentrating protein in grain powder, grain powder is treated with enzyme to increase the water-soluble saccharide content in a source, and by inoculating bacteria or yeast and fermentation, the increased water-soluble saccharide is removed, leading to a higher concentration of protein. Thus, the protein content ratio increase effects and the function of grain powder as a protein source are enhanced. 1. A method of concentrating protein in grain powder , the method comprising:treating grain powder with enzyme to decompose structural carbohydrate; andinoculating yeast into the grain powder to perform fermentation.2Saccharomyces.. The method of claim 1 , wherein the yeast is genus3. The method of claim 1 , wherein the grain powder is corn gluten.4. The method of claim 1 , wherein the fermentation is solid-state fermentation.5. The method of claim 1 , wherein the structural carbohydrate comprises at least one selected from the group consisting of starch claim 1 , cellulose claim 1 , hemicellulose claim 1 , or pectin.6. The method of claim 1 , wherein the enzyme is α-amylase or glucoamylase.7. The method of claim 1 , wherein the enzyme is used in an amount of 0.1 to 1 part by weight based on 100 parts by weight of the grain powder. This application is a Divisional of copending application Ser. No. 15/741,447, filed on Jan. 2, 2018, which was filed as PCT International Application No. PCT/KR2016/010705 on Sep. 23, 2016, which claims the benefit under 35 U.S.C. § 119(a) to Patent Application No. 10-2015-013660, filed in Korea on Sep. 25, 2015, and Patent Application No. 10-2016-0031463, filed in Korea on Mar. 16, 2016 all of which are hereby expressly incorporated by reference into the present application.The present ...

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Номер записи: 82
27-05-2021 дата публикации

COMPOSITIONS FOR GUT HEALTH

Номер: US20210153521A1
Автор: BENDEZU FELIPE, FRISCH RYAN, GRUBER TANJA, SAXER QUANCE GERDA
Принадлежит:

Provided herein, inter alia, are compositions of organic acid-producing microorganisms and methods of making and using the same to inhibit pathogenic bacterial populations in the gastrointestinal tracts of an animal and additionally promote improvement of one or more metrics in an animal, such as increased bodyweight gain, decreased feed conversion ratio (FCR), improved gut barrier integrity, reduced mortality, reduced pathogen infection, and reduced pathogen shedding in feces. 14-. (canceled)5Lactobacillus reuteriL. reuteriL. reuteriL. reuteriL. reuteriL. reuteriL. reuteriL. reuteriL. reuteri. A feed additive composition comprising a direct fed microbial (DFM) comprising one or more biologically pure strains of selected from the group consisting of (a) a biologically pure strain of displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a strain S1a deposited at Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS 147267; (b) a biologically pure strain of displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a strain S1b deposited at WFDB under number CBS 147268; (c) a biologically pure strain of displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a strain S2a deposited at WFDB under number CBS 147269; and (d) a biologically pure strain of displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a strain S2b deposited at WFDB under number CBS 147270.6L. reuteriL. reuteri. The feed additive composition of claim 5 , further comprising a biologically pure strain of displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a strain S3 deposited at WFDB under number CBS 145923.7L. reuteriL. reuteriL. reuteriL. reuteriL. reuteriL. reuteri. The feed additive composition of claim 6 , comprising a biologically pure strain of displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a strain S1a deposited at WFDB ...

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Номер записи: 83
27-05-2021 дата публикации

Animal Feed Compositions and Uses Thereof

Номер: US20210153523A1
Автор: Cohn Marianne Thorup, Kiemer Lars, Li Ming, Liu Ye, Sandvang Dorthe Hoej, Schnorr Kirk Matthew, Skov Lars Kobberoee
Принадлежит: NOVOZYMES A/S

The present invention relates animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity. The invention also relates to polypeptides having lysozyme activity, polynucleotides encoding the polypeptides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An animal feed or animal feed additive comprising one or more polypeptides having lysozyme activity , wherein the polypeptide comprises one or more GH24 catalytic domains and one or more lysozyme enhancing domains and wherein:(a) GH24 catalytic domain gives a domT score of at least 200 when queried using a Profile Hidden Markov Model prepared using SEQ ID NOs: 1 to 122 and hmmbuild software program, and wherein the query is carried out using hmmscan software program with default settings; and(b) the polypeptide comprises one or more lysozyme enhancing domains, wherein the lysozyme enhancing domain gives a domT score of at least 100 when queried using a Profile Hidden Markov Model prepared using SEQ ID NOs: 123 to 251 and hmmbuild software program, and wherein the query is carried out using the hmmscan software program with default settings.2. The animal feed or animal feed additive of claim 1 , wherein:(a) the GH24 catalytic domain comprises one or more motif I: [TAS][VIL][GAC][YFI]GHX[CAYIV] (SEQ ID NO: 252) and/or one or more motif II [LV][NDST]XN[QE][YFW][GASND]ALXS[WFLY]X[FY]N (SEQ ID NO: 253); and(b) the lysozyme enhancing domain comprises one or more motif III: [CGY][YF][VIL][ASTP][DG]X[YF][VIT]X[TS][GAN] (SEQ ID NO: 254).3. The animal feed or animal feed additive of claim 1 , wherein the lysozyme enhancing domain has at least 45% sequence identity to SEQ ID NO: 180.4. The animal feed or animal feed additive of claim 1 , wherein the lysozyme enhancing domain has at least 65% sequence identity to one or more of SEQ ID NO: 123 to 251.5. The animal feed or animal feed additive of claim 1 ...

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Номер записи: 84
16-04-2020 дата публикации

Xylanase Variants and Polynucleotides Encoding Same

Номер: US20200115691A1
Автор: Danielsen Steffen, Hansen Peter Kamp, Johansen Annette Helle, Pache Roland Alexander, Rasmussen Frank Winther
Принадлежит: NOVOZYMES A/S

The present invention relates to xylanase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the xylanase variants and methods of using the variants. 1. A method for obtaining a xylanase variant , comprising(a) introducing into a parent xylanase a substitution at one or more positions corresponding to positions 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 18, 19, 31, 32, 33, 34, 39, 43, 44, 45, 46, 48, 50, 58, 59, 61, 62, 64, 65, 67, 68, 79, 82, 88, 90, 94, 101, 102, 104, 110, 112, 113, 116, 119, 120, 123, 126, 127, 128, 129, 131, 135, 143, 145, 146, 159, 160, 165, 168, 176, 179, 181, 188, 191, 194, 195, 196, 197, 205, 209, 212, 217, 218, 221, 224, 231, 235, 237, 238, 242, 269, 280, 282, 295, 298, 299, 300, 302, 305, 306, 307, 311, 312, 313, 322, 323, 324, 333, 334, 335, 336, 337, 338, 339, 340, 342, 343, 344, 345, 346, 347, 349, 350, 354, 357, 359, 360, 363, 364, 366, 367, 368, 371, 373, 374, 376, 377, 378, 380, 385, 388, 390 and 391 of SEQ ID NO: 1, wherein the xylanase variant has xylanase activity and has at least 70%, e.g., at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to SEQ ID NO: 1; and(b) recovering the xylanase variant.2. The method of claim 1 , wherein the xylanase variant has improved thermostability relative to the parent.3. The method of claim 1 , wherein the parent xylanase has at least 70% claim 1 , e.g. claim 1 , at [[at]] least 75% claim 1 , at least 80% claim 1 , at least 85% claim 1 , at least 90% claim 1 , at least 95% claim 1 , at least 96% claim 1 , at least 97% claim 1 , at least 98% claim 1 , at least 99% or 100% sequence identity to SEQ ID NO: 1.4. The method of claim 1 , wherein the one or more substitutions is selected from the group consisting of A2D claim 1 , A2Q claim 1 , A2G claim 1 , A2W claim 1 , A2P claim 1 , A2L claim 1 , A2Y ...

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Номер записи: 85
10-05-2018 дата публикации

SYSTEMS AND METHODS FOR PRODUCING FERMENTED MILK REPLACER AND METHODS OF FEEDING SAME TO ANIMALS

Номер: US20180125093A1
Автор: Miller Bill L., Musser Robert C.
Принадлежит:

Young animals such as calves may be fed milk replacers containing soy having been preserved through treatment with acid-producing bacteria that reduces a pH of the milk replacer through microbial digestion of sugars present in soy to thereby produce a fermented milk replacer. The milk replacer is edible for at least 48 hours and its digestibility in the young animals is increased due to the microbial digestion of sugars in the soy that are otherwise indigestible in the gut of the young animals. The fermented milk replacers may be ingested by young animals in group settings. In addition, the milk replacers containing soy may be treated with enzymes adapted to hydrolyze complex indigestible carbohydrates within the milk replacer, potassium sorbate or both. Feeding systems incorporating these fermented milk replacers may result in increased gain and increased dry matter intake without negatively affecting animal health. 1. A method of feeding a young animal , comprising:in a tank or feeder, fermenting a liquid milk replacer comprising soy by treating the liquid milk replacer with an acid-producing bacteria; andfeeding the young animal the fermenting liquid milk replacer.2. The method of claim 1 , wherein treating the liquid milk replacer with an acid-producing bacteria comprises adding a starter culture of the acid-producing bacteria to the liquid milk replacer in the tank or feeder.3. The method of claim 1 , wherein treating the liquid milk replacer with an acid-producing bacteria comprises adding a starter culture of the acid-producing bacteria to the liquid milk replacer prior to adding the liquid milk replacer to the tank or feeder.4. The method of claim 1 , wherein the fermenting liquid milk replacer has a pH of about 4.0 to about 6.0 when fed to the young animal.5. The method of claim 1 , wherein the liquid milk replacer comprises potassium sorbate.6. The method of claim 5 , further comprising activating the potassium sorbate by reducing the pH of the liquid milk ...

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Номер записи: 86
10-05-2018 дата публикации

Buttiauxella sp. phytase variants

Номер: US20180125094A1
Автор: Birgitta Leuthner, Jayarama K Shetty, Klaus Schulze Pellengahr, Mai Faurschou Isaksen, Michael J. Pepsin, Oliver Kensch, Soeren Dalsgaard
Принадлежит: DANISCO US INC

Provided herein are variants of Buttiauxella sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds.

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Номер записи: 87
11-05-2017 дата публикации

GLUCANASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM

Номер: US20170130215A1
Автор: Callen Walter Niles, Healey Shaun, Pulliam Derrick, STEER Brian
Принадлежит:

The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4 -beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature. 1. An isolated , synthetic , or recombinant polypeptide comprising:(i) an amino acid sequence at least 70% or more or complete (100%) sequence identity to SEQ ID NO: SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:46, SEQ ID NO:48, SEQ ID NO:50, SEQ ID NO:52, SEQ ID NO:54, SEQ ID NO:56, SEQ ID NO:58, SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:64, SEQ ID NO:66, SEQ ID NO:68, SEQ ID NO:70, SEQ ID NO:72, SEQ ID NO:74, SEQ ID NO:76, SEQ ID NO:78, SEQ ID NO:80, SEQ ID NO:82, SEQ ID NO:84, SEQ ID NO:86, SEQ ID NO:88, SEQ ID NO:90, SEQ ID NO:92, SEQ ID NO:94, SEQ ID NO:96, SEQ ID NO:98, SEQ ID NO:100, SEQ ID NO:102, SEQ ID NO:104, SEQ ID NO:106, SEQ ID NO:108, SEQ ID NO:110, SEQ ID NO:112, SEQ ID NO:114, SEQ ID NO:116, SEQ ID NO:118, SEQ ID NO:120, SEQ ID NO:122, SEQ ID NO:124, SEQ ID NO:126, SEQ ID NO:128, SEQ ID NO:130, SEQ ID NO:132; SEQ ID NO:134; SEQ ID NO:136; SEQ ...

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Номер записи: 88
11-05-2017 дата публикации

PROCESSING BIOMASS

Номер: US20170130252A1
Автор: Medoff Marshall
Принадлежит:

Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids. 1. A method for processing biomass , the method comprising:combining a solid enzyme with a pretreated biomass.2. The method of claim 1 , wherein the method further comprises producing a product from the combination of the solid enzyme and the pretreated biomass.3. The method of claim 1 , further comprising feeding the combination to an animal.4. The method of claim 1 , wherein the method further comprises densifying the combination.5. The method of wherein the enzyme is selected from the group consisting of phytase claim 1 , cellulase claim 1 , lactase claim 1 , lipase claim 1 , pepsin claim 1 , catalase claim 1 , xylanase claim 1 , and pectinase.6. The method of claim 1 , wherein the enzyme comprises a cellulase.7. The method of claim 1 , wherein the biomass comprises a cellulosic or lignocellulosic material.8. The method of claim 1 , wherein the biomass is selected from the group consisting of paper claim 1 , paper products claim 1 , paper waste claim 1 , wood claim 1 , particle board claim 1 , sawdust claim 1 , agricultural waste claim 1 , sewage claim 1 , silage claim 1 , grasses claim 1 , rice hulls claim 1 , bagasse claim 1 , jute claim 1 , hemp claim 1 , flax claim 1 , bamboo claim 1 , sisal claim 1 , abaca claim 1 , straw claim 1 , corn cobs claim 1 , corn stover claim 1 , switchgrass claim 1 , alfalfa claim 1 , hay claim 1 , coconut hair claim 1 , cotton claim 1 , seaweed claim 1 , algae claim 1 , and mixtures thereof.9. The method of wherein the native biomass material includes a genetically modified plant material.10. The method of claim 2 , wherein the method comprises adding water to the combination and then sacharifying the combination to produce the product comprising sugars.11. The method of claim 1 , wherein the pretreated biomass is pretreated using one or more of size reduction claim ...

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Номер записи: 89
02-05-2019 дата публикации

Compositions Comprising Polypeptides having Galactanase Activity and Polypeptides having Beta-Galactosidase Activity

Номер: US20190127717A1
Автор: Carstensen Lone, Gjermansen Morten, Krogh Kristian B.R.M., Salomon Jesper, Spodsberg Nikolaj
Принадлежит: NOVOZYMES A/S

The present invention relates to compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity for use in e.g. animal feed. The present invention further relates to polypeptides having beta-galactosidase activity, polypeptides having galactanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. A composition comprising one or more GH53 polypeptides having galactanase activity and one or more GH42 polypeptides having beta-galactosidase activity.2. The composition of claim 1 , wherein the GH53 polypeptide comprises the motif GV[T/M]PD[W/M]VQ[I/V]GNE (SEQ ID NO: 81) and/or the motif WADP[A/G]xQxKPxAW (SEQ ID NO: 82).3. The composition of claim 1 , wherein the GH53 polypeptide and the GH42 polypeptide are obtained or obtainable from the taxonomic order Bacillales.4. The composition of wherein the GH53 polypeptide is selected from the group consisting of:(a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 3;(b) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 7;(c) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 11;(d) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 15;(e) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 19;(f) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 23;(g) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 27;(h) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 31;(i) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 35;(j) a polypeptide having at least 80% sequence identity to the polypeptide of ...

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Номер записи: 90
18-05-2017 дата публикации

COMPOSITION AND USE THEREOF

Номер: US20170135378A1
Автор: AWATI AJAY, Frouel Stephane, Tse Kathryn Louise
Принадлежит:

The present invention relates to a composition comprising a hydro-soluble component and a coating substance, wherein the hydro-soluble component is coated with the coating substance, wherein the coating substance reduces leaching of the hydro-soluble component from the composition when the composition is contacted with aqueous media. Furthermore, the present invention relates to uses of said composition and an aquafeed comprising said composition. 1. A composition comprising a hydro-soluble component and a coating substance , wherein the hydro-soluble component is coated with the coating substance , wherein the coating substance reduces leaching of the hydro-soluble component from the composition when the composition is contacted with aqueous media.2. The composition of wherein the hydro-soluble component is selected from the group consisting of betaine claim 1 , direct fed microbials claim 1 , enzymes claim 1 , essential oils claim 1 , organic acids claim 1 , vitamins claim 1 , amino acids and minerals.3. The composition of or wherein the hydro-soluble component comprises betaine or an animal feed acceptable salt or hydrate thereof.4. The composition of wherein the coating substance comprises a lipid claim 3 , an emulsifier or a polymer.5. The composition of wherein the coating substance comprises a polymer selected from film-forming polysaccharide or protein selected from one or more of the group of cellulosic polymers (methyl cellulose claim 4 , carboxymethyl cellulose claim 4 , hydroxypropylmethyl cellulose claim 4 , ethylcellulose claim 4 , hydroxyethylcellulose claim 4 , hydroxypropylcellulose) claim 4 , sodium alginate claim 4 , gum arabic claim 4 , gellan gum claim 4 , starch claim 4 , modified starch claim 4 , guar gum claim 4 , agar gum claim 4 , pectin claim 4 , amidified pectin claim 4 , carrageenan claim 4 , gelatine claim 4 , chitosan claim 4 , mesquite gum claim 4 , hyaluronic acid claim 4 , whey protein claim 4 , soy protein claim 4 , sodium ...

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Номер записи: 91
17-05-2018 дата публикации

Use of Polypeptides Having Protease Activity in Animal Feed and Detergents

Номер: US20180135036A1
Автор: Astrid Benie, Carsten Sjoeholm, Katrine Fruegaard Pontoppidan, Morten Gjermansen, Peter Rahbek Oestergaard, Robert Piotr OLINSKI, Tine Hoff
Принадлежит: Novozymes AS

The present invention relates to the use of isolated polypeptides having protease activity in animal feed and detergents. It also relates to the use of isolated nucleic acid sequences encoding the proteases in the recombinant production of isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, particularly using the proteases in animal feed and detergents.

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Номер записи: 92
30-04-2020 дата публикации

Polypeptides having Lysozyme Activity, Polynucleotides Encoding Same and Uses and Compositions Thereof

Номер: US20200131492A1
Автор: Li Ming, Liu Ye, Olsen Peter Bjarke, Schnorr Kirk Matthew
Принадлежит: NOVOZYMES A/S

Animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity. Polypeptides having lysozyme activity, polynucleotides encoding the polypeptides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 120-. (canceled)21. A composition comprising at least 0.01 mg of LYS polypeptide per kilogram of composition , wherein the polypeptide (a) has lysozyme activity and (b) comprises one or more LAD catalytic domains; wherein the LAD catalytic domain gives a domT score of at least 180 when queried using a Profile Hidden Markov Model (HMM) prepared using SEQ ID NOs: 46 to 187 and hmmbuild software program.22. The composition of claim 21 , wherein the polypeptide further comprises one or more lysozyme enhancing domains claim 21 , wherein the lysozyme enhancing domain gives a domT score of at least 100 when queried using a Profile Hidden Markov Model prepared using SEQ ID NOs: 188 to 316 and hmmbuild software program claim 21 , and wherein the query is carried out using the hmmscan software program.23. The composition of claim 21 , wherein the polypeptide is selected from the group consisting of:(a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 3;(b) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 6;(c) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 9;(d) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 12;(e) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 15;(f) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 18;(g) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 21;(h) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 24;(i) a polypeptide having at least 80% ...

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Номер записи: 93
25-05-2017 дата публикации

BUTTIAUXELLA SP. PHYTASE VARIANTS

Номер: US20170143004A1
Автор: Dalsgaard Søren, ISAKSEN MAI FAURSCHOU, Kensch Oliver, Leuthner Birgitta, Pellengahr Klaus Schulze, Pepsin Michael, Shetty Jayarama K.
Принадлежит: DANISCO US INC.

Provided herein are variants of sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds. 1Buttiauxella. A phytase variant having improved thermal activity of at least about 5° C. as compared to wild type sp. phytase (SEQ ID NO: 1).2. The phytase variant of claim 1 , wherein the improved thermal activity is at least about 17° C. as compared to the phytase SEQ ID NO: 1.3. The phytase variant of or claim 1 , wherein the improved thermal activity is in a range from about 17 to about 21° C.4. The phytase variant of any one of to claim 1 , wherein the improved thermal activity is in a range from about 17.4 to about 20.2° C.5Buttiauxella. A thermostable phytase variant capable of retaining greater than 50% of its activity after exposure to an elevated temperature for 10 minutes at pH 5.5 in buffer claim 1 , wherein the elevated temperature is at least about 5° C. higher than a temperature at which wild type sp. phytase (SEQ ID NO: 1) retains greater than 50% of its activity.6. The thermostable phytase variant of claim 5 , wherein the elevated temperature is at least about 20.5° C. higher.7. The thermostable phytase variant of or claim 5 , wherein the elevated temperature is in a range from about 20.5° C. to about 27° C.8. The thermostable phytase variant of any one of to claim 5 , wherein the elevated temperature is in a range from about 20.2° C. to about 26.8° C.9Buttiauxella. A phytase variant having phytase activity and an amino acid sequence that varies from the amino acid sequence of wild type sp. phytase (SEQ ID NO: 1) claim 5 , wherein the amino acid sequence of the phytase variant comprises a variation at one or more positions corresponding to position 75 claim 5 , 76 claim 5 , 77 claim 5 , 198 claim 5 , 367 or 374 of SEQ ID NO: 1.10. The phytase variant of claim 9 , wherein the phytase variant further comprises one or more additional ...

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Номер записи: 94
25-05-2017 дата публикации

Composition and Use Thereof

Номер: US20170143006A1
Автор: Ajay AWATI, Kathryn Louise Tse, Rafael Duran Gimenez-Rico, Stéphane FROUEL, Tracey Sainsbury
Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

The present invention relates to a composition comprising a feed or feed additive and a coating substance, wherein the feed or feed additive is coated with the coating substance, wherein the coating substance decreases the degree of degradation of the feed or feed additive within the rumen environment. Furthermore, the present invention relates to the use of said compositions for increasing feed utilization, improving animal nutrition, milk production and reducing global warming.

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Номер записи: 95
25-05-2017 дата публикации

NON-DIGESTIBLE SUGAR-COATED PRODUCTS AND PROCESS

Номер: US20170143638A1
Автор: Miller Guy W.
Принадлежит:

A method and composition are provided for coating a component to achieve colon-targeted delivery. A component is coated with a fructose-based non-digestible carbohydrate such as a inulin, fructo-oligosaccharide or neosugar. The coated component is orally administered to a monogastric animal. The non-digestible coating causes the composition to pass through the stomach and small intestine without being degraded, and delivers the component to the colon where the coating is digested by microbial fermentation and the component is released. 1. A method of delivering a component to the colon of an animal comprising coating the component with a fructose-based non-digestible carbohydrate; and providing the coated component for oral administration to the animal.2. The method of wherein the fructose-based non-digestible carbohydrate is fructan.3. The method of wherein the fructose-based non-digestible carbohydrate is fructo-oligosaccharide.4LactobacillusBifidobacterium bacteria. The method of wherein the component comprises or ; and one or more of a mineral claim 1 , vitamin claim 1 , drug claim 1 , bacteria claim 1 , yeast claim 1 , immune stimulator claim 1 , nutrient claim 1 , nutraceutical claim 1 , electrolyte claim 1 , chelated mineral claim 1 , mold claim 1 , enzyme claim 1 , energy-providing compound claim 1 , antibody claim 1 , or acid.5. The method of wherein the component is a nutraceutical.6. The method of wherein the component is an enzyme.7. The method of wherein the component is an immune stimulator.8. The method of wherein the component is a drug.9. The method of wherein the coating step comprises applying powdered fructose-based non-digestible carbohydrate with a liquid to form a thin film coating on the component.10. The method of wherein the coating step comprises combining the fructose-based non-digestible carbohydrate with a liquid to form a mixture and atomizing and spraying the mixture on the component to form a thin film coating on the component.11. ...

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Номер записи: 96
07-05-2020 дата публикации

Microbials for animals

Номер: US20200138877A1
Автор: Elizabeth GALBRAITH, Michael R. King, Ricardo P. ARIAS, Samanta R. FENSTERSEIFER, Sona SON
Принадлежит: Microbial Discovery Group LLC, United Animal Health Inc

The invention relates to direct-fed microbials for use in improving reproductive performance in animals, decreasing death loss and/or off-feed events in animals, improving milk production and/or milk quality in dairy animals, and inhibiting a disease or disorder in dairy animals selected from the group consisting of milk fever, ketosis, retained placenta, metritis, pneumonia, and displaced abomasum. More particularly, the invention relates to isolated Bacillus strains 86, 300, 101, 235, 177, and 102, and strains having all of the identifying characteristics of these strains, for uses comprising the above-mentioned uses.

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Номер записи: 97
15-09-2022 дата публикации

ANTIMICROBIAL ENDOLYSIN POLYPEPTIDES, COMPOSITIONS AND FORMULATIONS

Номер: US20220290114A1
Автор: LEE Weiluo, MILLER Kane, NAVARRO Francisco J.
Принадлежит:

The present invention relates to novel endolysin polypeptides or fragments thereof which possess antimicrobial activity, preferably antibacterial activity against . The invention also relates to a nucleic acid molecule encoding the endolysin polypeptide or fragment, a recombinant polynucleotide expression vector comprising such a nucleic acid molecule, as well as a host cell comprising such a nucleic acid molecule or comprising such a recombinant polynucleotide expression vector. The invention also relates to compositions and foodstuffs comprising the endolysin polypeptides or fragments and uses thereof in the treatment of diseases or disorders in animals. 120-. (canceled)21. An algal host cell comprising a population of nucleic acid molecules encoding an endolysin polypeptide , wherein each one of the molecules of the population encodes the same endolysin polypeptide or encode two or more different endolysin polypeptides , optionally wherein the host cell additionally comprises an endolysin polypeptide which has been expressed from said molecules; wherein the polypeptide has antimicrobial activity and comprises an amino acid sequence:{'i': 'Clostridium perfringens', 'which is an N-terminal cell wall peptidoglycan catalytic domain polypeptide,'}or which is a fragment of said N-terminal catalytic domain polypeptide; or{'i': 'Clostridium perfringens', 'which is at least 80% identical to the amino acid sequence of the N-terminal cell wall peptidoglycan catalytic domain of the bacteriophage endolysin polypeptide set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8 or 11,'}or which is a fragment of said N-terminal catalytic domain polypeptide, and which is at least 80% identical to the amino acid sequence of the sequence set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8 or 11 which corresponds to the amino acid sequence of the fragment.22. (canceled)23. A cell lysate of a population of algal cells of claim 21 , wherein the algal cells of the population comprise said optional ...

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Номер записи: 98
07-05-2020 дата публикации

PHYTASE VARIANTS YEAPPA HAVING IMPROED PEPSIN RESISTANCE AND ACID RESISTANCE, AND INCREASED CATALYTIC EFFICIENCY

Номер: US20200140833A1
Автор: DU Yongkai, HUANG Huoqing, Li Yangyang, LUO Huiying, NIU Canfang, WANG Yaru, YANG PEILONG, Yao Bin
Принадлежит:

The present invention relates to the field of genetic engineering, particularly to phytase variant YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency, by substituting Leucine at the 162site of the sequence set forth in SEQ ID NO.1 with glycine or proline or substituting glutamic acid at the 230site of the sequence set forth in SEQ ID NO.1 with glycine, proline or arginine, in the benefit of the development of economical feed enzyme industry. 1. Phytase YeAPPA variant with the following characteristics of having amino acid sequence substituting Leucine at the 162th site of the sequence set forth in SEQ ID NO.1 with glycine or proline , and having improved pepsin resistance and acid resistance , and increased catalytic efficiency.2. Phytase YeAPPA variant with the following characteristics of having amino acid sequence substituting glutamic acid at the 230th site of the sequence set forth in SEQ ID NO.1 with glycine , proline or arginine , and having improved pepsin resistance and acid resistance , and increased catalytic efficiency.3. A polynucleotide encoding the phytase YeAPPA variant of .4. Polynucleotide according to claim 3 , is characterized of having the nucleotide sequence set in forth in SEQ ID NO.8 claim 3 , SEQ ID NO.9 claim 3 , SEQ ID NO.10 claim 3 , SEQ ID NO.11 or SEQ ID NO. 12 claim 3 , respectively.5. DNA constructor comprising the polynucleotide of .6. Recombinant cell comprising the polynucleotide of .7. A method of producing a phytase variant comprising the steps of transforming host with DNA constructor of to obtain recombinant host cell; cultivating the recombinant host cell to produce the supernatant containing phytase variant; and recovering the said phytase variant.8. Use of the phytase variant of . The present invention relates to the field of genetic engineering, particularly to phytase variants YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency. ...

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Номер записи: 99
22-09-2022 дата публикации

METHODS OF INHIBITION WITH MICROBIAL STRAINS AND ANTIBIOTICS

Номер: US20220295828A1
Автор: KING Michael R., Leistikow Kyle, SON Sona
Принадлежит:

The invention relates to direct-fed microbials for use in and/or inhibition in animals in combination with antibiotics. More particularly, the invention relates to isolated strains 2, 3, 4, 5, 6, 7, 9, 57, 71, and 126, and strains having all of the identifying characteristics of these strains, for a use comprising the above-mentioned use. 1BacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillusBacillus. A method of feeding an animal , the method comprising the step of administering to the animal a feed composition or drinking water comprising an effective amount of an additive comprising an isolated strain selected from the group consisting of strain 2 (NRRL No. B-67709) , a strain having all of the identifying characteristics of strain 2 (NRRL No. B-67709) , strain 3 (NRRL No. B-67710) , a strain having all of the identifying characteristics of strain 3 (NRRL No. B-67710) , strain 4 (NRRL No. B-67711) , a strain having all of the identifying characteristics of strain 4 (NRRL No. B-67711) , strain 5 (NRRL No. B-67712) , a strain having all of the identifying characteristics of strain 5 (NRRL No. B-67712) , strain 6 (NRRL No. B-67714) , a strain having all of the identifying characteristics of strain 6 (NRRL No. B-67714) , strain 7 (NRRL No. B-67713) , a strain having all of the identifying characteristics of strain 7 (NRRL No. B-67713) , strain 9 (NRRL No. B-67866) , a strain having all of the identifying characteristics of strain 9 (NRRL No. B-67866) , strain 57 (NRRL No. B-67870) , a strain having all of the identifying characteristics of strain 57 (NRRL No. B-67870) , strain 71 (NRRL No. B-67867) , a strain having all of the identifying characteristics of strain 71 (NRRL No. B-67867) , strain 126 (NRRL No. B-67868) , a strain having all of the identifying characteristics of strain 126 (NRRL No. B-67868) , and combinations thereof.2. The method of wherein the ...

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Номер записи: 100