17-02-2022 дата публикации
Номер: US20220049230A1
Принадлежит:
NOVOZYMES A/S
The present invention relates to phytase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. 1. A phytase variant which has at least 70% identity to SEQ ID NO: 2 and which comprises the alterations N31C/G52C/A99C/K141C/T177C/V199C/N203L as compared to SEQ ID NO: 2 and further comprises a substitution in one or more position(s) selected from the following: 30 , 36 , 43 , 46 , 57 , 60 , 64 , 73 , 79 , 119 , 121 , 123 , 130 , 134 , 138 , 151 , 155 , 161 , 162 , 168 , 176 , 180 , 184 , 190 , 207 , 224 , 230 , 243 , 273 , 286 , 336 , 340 , 358 and 375 using SEQ ID NO: 2 for numbering.2. The variant of claim 1 , comprising substitutions in the positions: 57 claim 1 , 73 claim 1 , 121 claim 1 , 134 claim 1 , 155 claim 1 , 207 and 273.3. The variant of claim 2 , further comprising substitutions in the positions: 36 claim 2 , 60 claim 2 , 64 claim 2 , 73 claim 2 , 119 claim 2 , 130 claim 2 , 138 claim 2 , 161 claim 2 , 162 claim 2 , 168 claim 2 , 176 claim 2 , 180 claim 2 , 184 claim 2 , 190 claim 2 , 224 claim 2 , 230 claim 2 , 243 claim 2 , 336 and 340.4. The variant of claim 1 , where the substitutions are selected among: 30Q claim 1 , 36A claim 1 , 43C claim 1 , 46C claim 1 , 57Y claim 1 , 60H claim 1 , 64Q claim 1 , 73P claim 1 , 79Q claim 1 , 119P claim 1 , 121P claim 1 , 123C claim 1 , 130T claim 1 ,C claim 1 , 134Q claim 1 , 138A claim 1 , 151S claim 1 , 155F claim 1 , 161T claim 1 , 162A claim 1 , 168R 176P claim 1 , 180N claim 1 , 184Q claim 1 , 190T claim 1 , 207T claim 1 , 224Q claim 1 , 230E claim 1 , 243N claim 1 , 273L claim 1 , 286S claim 1 , 336R claim 1 , 340L claim 1 ,P claim 1 , 358Q and 375K.5. The variant of claim 1 , comprising the substitutions 31C/52C/57Y/73P/99C/121P/134Q/141C/155F/177C/199C/203L/207T/273L.6. The variant of claim 5 , selected among variants comprising the substitutions selected from ...
Подробнее