УСТРОЙСТВО ДЛЯ ЭЛЮИРОВАНИЯ РАДИОАКТИВНОГО МАТЕРИАЛА

Заявленное изобретение относится к устройству для элюирования радиоактивного материала. Заявленное устройство (100) для элюирования радиоактивного материала (160) может содержать элюционную колонку (105), предназначенную для размещения в ней радиоактивного материала, первый уплотнительный элемент (110), уплотняющий первый конец (111) элюционной колонки (105), второй уплотнительный элемент (120), уплотняющий второй конец (112) элюционной колонки (105), источник (20) подачи элюирующего вещества, соединенный с первым концом (111) элюционной колонки (105) при помощи первой иглы (22), устройство (40) сбора, соединенное со вторым концом (112) элюционной колонки (105) при помощи второй иглы (42), и фильтр (150), расположенный в элюционной колонке (105) и предназначенный для поддержания радиоактивного материала (160) и предотвращения контакта указанного материала (160) со второй иглой (42). Техническим результатом является возможность регулирования эффективности собирания ионов в процессе элюирования ...

ГЕОМЕТРИЯ КОЛОНКИ ДЛЯ ПОВЫШЕНИЯ ЭФФЕКТИВНОСТИ ЭЛЮИРОВАНИЯ МОЛИБДЕНА-99

... 1. Устройство (100) для элюирования радиоактивного материала (160), ! содержащее: ! элюционную колонку (105, 105', 105"), предназначенную для размещения в ней радиоактивного материала (160), ! первый уплотнительный элемент (110), уплотняющий первый конец (111) элюционной колонки (105), ! второй уплотнительный элемент (120), уплотняющий второй конец (112) элюционной колонки (105), ! источник (20) подачи элюирующего вещества, соединенный с первым концом (111) элюционной колонки (105) при помощи первого проточного канала (22), ! устройство (40) сбора, соединенное со вторым концом (112) элюционной колонки (105) при помощи второго проточного канала (42), и ! фильтр (150), расположенный в элюционной колонке (105) и предназначенный для поддержания радиоактивного материала (160) и предотвращения контакта указанного материала (160) с иглой (42). ! 2. Устройство по п.1, в котором длина (L) элюционной колонки (105) составляет приблизительно 10 5/8 дюйма (27 см), а внутренний диаметр (D) указанной ...

Chromatography columns,systems and methods

Fluid separation with sampling unit selectively coupling upstream and downstream of separation unit

A method for processing fluid in a fluid separation path wherein a mobile phase is driven through a separation unit (30) such as a liquid chromatography column for separating a fluidic sample when comprised within the first mobile phase. The method comprises: in a first state (A), introducing fluid into a modulation buffering unit (220) from downstream of the separation unit (30), and in a second state (B), introducing fluid buffered in the modulation buffering unit (220) into the mobile phase for being separated by first separation unit (30). A fluid processing apparatus (100) for enabling the method is also disclosed. The method and apparatus provide for fluid separation with a reduction in the total cost of the apparatus by elimination of redundancy and reduction of total parts count and enhance reliability and robustness due to reduction of total parts count.

A PROCESS AND APPARATUS FOR PURIFICATION OF WATER

A PROCESS AND APPARATUS FOR PURIFICATION OF WATER

Multi-stage separation device for use with flowable system of substances

A multi-stage separation device for separating a first fluid from at least one other second substance, the first fluid and second substance forming a flowable system of substances. The device comprising a housing having a substantially cylindrical form about a central axis with a wall disposed between a first end and second end, and an inlet disposed near said first end of the housing and an outlet in the second end. The wall when viewed in cross section perpendicular to the central axis has an ever decreasing radius spiralling between at least a first edge of said wall and a second edge of the wall. The first edge and second edge form part of the periphery of an inlet in the housing. At least one permeable cylindrical separation module is disposed within the housing.

INFLUENCING A SEQUENTIAL CHROMATOGRAPHY IN REAL-TIME

What is disclosed herein describes a system and a method for influencing a sequential chromatography.

STERILE CHROMATOGRAPHY RESIN AND USE THEREOF IN MANUFACTURING PROCESSES

Provided herein are methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and at least one chelator and/or antioxidant agent, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified ...

CENTRIFUGATION PROCEDURE AND - DEVICE FOR PROCESSING FLUID MATERIALS.

DOUBLE FLOW CHROMATOGRAPHY SYSTEM

The disclosed invention relates to a new liquid chromatographic method, wherein the flow direction of sample is directed alternately in an essentially horizontal direction and in an essentially vertical direction through a separating matrix having a dimensional ratio between the column width and column height of at least 1:0.5, preferably at least 1:0.2, more preferably at least 1:0.1 , even more preferably at least 1:0.04. The invention further relate to an apparatus and a column design for controlling the flow direction of a liquid sample through a separating matrix in either an essentially horizontal or in an essentially vertical direction.

MULTI COLUMN CHROMATOGRAPHY SYSTEM

The present invention relates to a method and apparatus for chromatographically analyzing each of a plurality of samples in detector, comprising an autosampler to contain a plurality of samples for chromatographic analysis and a plurality of chromatographic systems, each system comprising one or more pumps and one or more chromatography columns. A detector is included for detecting compounds in the samples from each of the chromatography systems along with a valve positioned between the detector and the plurality of chromatography systems, the valve permitting each sample to reach the detector in sequence. A computer control device is included which adjusts the introduction of samples from the autosampler into the plurality of chromatography systems as well as the position of the valve to sequentially separate and deliver compounds within the samples to the detector.

RADIAL CHROMATOGRAPHY FOR CARBOHYDRATE SEPARATION

The invention relates to a process for separating one or more carbohydrate from a composition wherein separating is done through radial chromatography. Preferably, the invention relates to a process for separating at least two carbohydrates from a composition wherein separating is done through radial chromatography, and wherein each of the at least two carbohydrates are collected in a purified form. The present invention relates to the use of radial chromatography for the separation of one or more carbohydrate from a composition and obtaining the one or more carbohydrate in a purified form.

МНОГОСТАДИЙНЫЙ СПОСОБ РАЗДЕЛЕНИЯ

Изобретение относится к области хроматографии. Предложен способ хроматографического разделения для получения продукта полиненасыщенных жирных кислот (PUFA) из подаваемой смеси. Способ включает: (a) очистку подаваемой смеси на первом этапе хроматографического разделения с использованием в качестве элюента смеси воды и первого органического растворителя с получением промежуточного продукта и (b) очистку промежуточного продукта на втором этапе хроматографического разделения с использованием в качестве элюента смеси воды и второго органического растворителя с получением PUFA-продукта, где второй органический растворитель отличается от первого органического растворителя и имеет индекс полярности, который отличается от индекса полярности первого органического растворителя в пределах от 0,3 до 1,5, где PUFA-продукт представляет собой по меньшей мере одну ω-3-PUFA или по меньшей мере одно производное ω-3-PUFA, и где PUFA-продукт не является альфа-линоленовой кислотой (ALA), моно-, ди- или триглицеридом ...

Modulares automatisiertes Chromatografiesystem

System zum Verbinden mehrerer Fluidmanipulationskomponenten zu einem Strömungsschema zum Leiten von Fluiden zu und von einer chromatografischen Trennvorrichtung und zum Betreiben der verbundenen Fluidmanipulationskomponenten gemäß einem ausgewählten Protokoll, wobei das System aufweist:mehrere Module, wobei jedes Modul (a) eine der Fluidmanipulationskomponenten und (b) einen Mikrocontroller aufweist, der in Reaktion auf von außerhalb des Moduls empfangene Befehle an die Fluidmanipulationskomponente überträgt;ein Montagegestell mit mehreren Schächten, wobei jeder der mehreren Schächte zum Aufnehmen eines einzelnen Moduls konstruiert ist, und wobei jeder der mehreren Schächte einen Signalverbinder aufweist, der eine Verbindung mit dem Mikrocontroller eines in dem Schacht angebrachten Moduls herstellt und mit diesem kommuniziert, wobei zumindest einige der Schächte derart konstruiert sind, dass sie einzelne Module mit anderen Modulen austauschbar aufnehmen; undeine Softwareplattform in Verbindung ...

Improved process for purification of 6 acetyl 4,1',6' trichlorogalactosucrose and 4,1'6' trichlorogalactosucrose by chromatography on silanized silica gel

Method for high throughput volumes in the fractionation of bio-molecules by chromatographic systems

Influencing a sequential chromatography in real-time

What is disclosed herein describes a system and a method for influencing a sequential chromatography.

IMPROVED LIQUID HANDLING FOR FILTRATION AND PREPARATIVE CHROMATOGRAPHY

A method and system are provided for high-precision separation of pharmaceutical or biotechnology liquids. The separation can be in accordance with direct flow filtration, tangential flow filtration or preparative chromatography. Movement of the pharmaceutical or biotechnology liquid within a flow path is controlled according to a selected pattern. Selected patterns include automatically and progressively increasing the flow rate within the unit, automatically and progressively increasing the pressure within the unit or initially proceeding according to a relatively high constant flow rate and switching to a relatively high constant pressure at a time when a given parameter is attained.

FLOW DISTRIBUTION APPARATUS

AN AUTOMATED INSTALLATION PROCEDURE FOR A DISPOSABLE FLOW PATH

This invention provides an automated installation procedure for assembling a disposable flow path: providing a disposable flow path comprising tubing and a plurality of sensors onto a re-usable instrument; qualifying said tubing and said plurality of sensors to be on the flow path based on a standard; and determining if the tubing and the plurality of sensors comply with characteristics and performance according to limits for specifications or acceptance criteria.

PURIFICATION OF ANTIBODIES USING SIMULATED MOVING BED CHROMATOGRAPHY

The present invention relates to compositions and methods for the chromatographic purification of antibodies, such as monoclonal antibodies, employing improved simulated moving bed separation strategies and, in certain embodiments, Raman spectroscopy.

HEATED CHROMATOGRAPHIC SEPARATION PROCESS

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which process comprises passing the feed mixture through one or more chromatographic columns containing, as eluent, an aqueous organic solvent, wherein the temperature of at least one of the chromatographic columns through which the feed mixture is passed is greater than room temperature.

SMB PROCESS FOR PRODUCING HIGHLY PURE EPA FROM FISH OIL

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture which is a fish oil or which is derived from fish oil, which process comprises the steps of: (i) purifying the feed mixture in a chromatographic separation step, to obtain a first intermediate product; and (ii) purifying the first intermediate product obtained in (i) in a simulated or actual moving bed chromatographic separation step, to obtain a second intermediate product; and (iii) purifying the second intermediate product obtained in (ii) in a simulated or actual moving bed chromatographic separation step, to obtain the PUFA product; wherein an aqueous organic solvent is used as eluent in each separation step; saturated and/or monounsaturated fatty acids present in the feed mixture are removed in the first separation step; the PUFA product is separated from different components of the feed mixture in steps (ii) and (iii); and the PUFA product ...

CHROMATOGRAPHIC PURIFICATION OF POLYUNSATURATED FATTY ACIDS

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which comprises: (a) purifying the feed mixture in a first chromatographic separation step using as eluent a mixture of water and a first organic solvent, to obtain an intermediate product; and (b) purifying the intermediate product in a second chromatographic separation step using as eluent a mixture of water and a second organic solvent, to obtain the PUFA product, wherein the second organic solvent is different from the first organic solvent and has a polarity index which differs from the polarity index of the first organic solvent by between 0.1 and 2.0, wherein the PUFA product is other than alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), linoleic acid, an ALA mono- di- or triglyceride, a GLA mono- di- or triglyceride, a linoleic acid mono, di- or triglyceride, an ALA C1 -C4 alkyl ester, a GLA C1 -C4 alkyl ester or a linoleic acid ...

CHROMATOGRAPHIC PROCESS FOR THE PRODUCTION OF HIGHLY PURIFIED POLYUNSATURATED FATTY ACIDS

The invention relates to a process for recovering a first polyunsaturated fatty acid from a feed mixture, wherein the feed mixture comprises at least a second fatty acid in addition to the first polyunsaturated fatty acid, the process comprising: - performing a main step of chromatographic separation using an aqueous organic eluent and thereby collecting a first stream of eluent enriched in the first polyunsaturated fatty acid and a second stream of eluent enriched in the second fatty acid; - subjecting the second stream of eluent to a concentration step so as to obtain a stream of concentrated fatty acids on the one hand and a depleted second stream of eluent on the other hand, wherein the water-to-organic ratio of the depleted second stream of eluent is lower than the water-to-organic ratio of the second stream of eluent; recycling at least part of the depleted second stream of eluent to use it in the main step of chromatographic separation.

Procédé d'extraction d'un produit par centrifugation.

L'INVENTION SE RAPPORTE AUX APPLICATIONS DE LA CENTRIFUGATION. ELLE CONCERNE UN PROCEDE D'EXTRACTION D'UN PRODUIT A PARTIR D'UNE PREMIERE PHASE, CARACTERISE EN CE QU'IL COMPREND LES ETAPES SUIVANTES: ON FAIT PASSER PAR CENTRIFUGATION LA PREMIERE PHASE A TRAVERS DES MOYENS DE SEPARATION POUR EXTRAIRE LE PRODUIT A PARTIR DE LA PREMIERE PHASE; ON ELUE LA MATIERE EXTRAITE EN FAISANT PASSER PAR CENTRIFUGATION UN SOLVANT DE CETTE MATIERE A TRAVERS LES MOYENS DE SEPARATION; ET ON RECUEILLE LE PRODUIT ENTRAINE PAR LE SOLVANT. UTILISATION A DES FINS D'ANALYSE BIOLOGIQUE.

Prefilled liquid cartridge for the supply of a sample separation device with an operating liquid

Prefilled liquid cartridge for fluidically connecting to a sample separation device for separating of components of a fluidic sample by using liquid of the liquid cartridge, wherein the liquid cartridge comprises a liquid container which is prefilled with liquid and a liquid removal access provided at the liquid container, adapted to be fluidically coupled with at least one liquid conduit of the sample separation device by only inserting the liquid cartridge in a corresponding liquid cartridge accommodation of the sample separation device.

PURGE METHOD FOR LOW PRESSURE GRADIENT FORMATION LIQUID CHROMATOGRAPHY

Described is a method for purging a fluid channel is a low pressure gradient formation liquid flow system. Control of the fluid channels for multiple solvents allows for one or more static volumes of solvents not intended for use in an isocratic flow to be purged from their fluid channels to avoid contamination of the isocratic solvent. Advantageously, the method avoids the need to modify equipment or to reconfigure a pumping system so that the inlet is directly coupled to a single solvent source. Thus there is no need to bypass existing valves and liquid coupling components where solvents are combined during conventional gradient operation.

SYSTEM AND PROCESS FOR BIOPOLYMER CHROMATOGRAPHY

СПОСОБ (ВАРИАНТЫ) ЗАХВАТА ПРЕДСТАВЛЯЮЩИХ ИНТЕРЕС ЧАСТИЦ ИЗ СМЕСИ

Настоящее изобретение относится к способу захвата представляющих интерес вирусоподобных частиц из смеси, включающей разрушенные клетки растений. Способ включает использование расширяющегося слоя адсорбента, содержащего материал смолы, уравновешивание материала смолы при рН 6,0-8,0 и внесение смеси на расширяющийся слой адсорбента для связывания вирусоподобных частиц. Степень расширения расширяющегося слоя равна 1-5. Далее адсорбент отмывают. Вирусоподобные частицы элюируют из адсорбента. Технический результат: обеспечение высокой чистоты выделяемых частиц, высокойя эффективности процесса. 4 н. и 13 з.п. ф-лы, 9 табл.

ОЧИСТКА АНТИТЕЛ С ПОМОЩЬЮ ХРОМАТОГРАФИИ С ПСЕВДОДВИЖУЩИМСЯ СЛОЕМ

Изобретение относится к области биохимии. Описан способ получения препарата белка из образца смеси, содержащей белок, являющийся целью выделения, и по меньшей мере один НСР. Способ включает в себя стадии проведения анализа Рамановской спектроскопии указанного образца смеси; приведение указанного образца смеси в контакт с аффинной хроматографической смолой в хроматографическом разделении с псевдодвижущимся слоем; сбор хроматографического образца; и проведение анализа Рамановской спектроскопии указанного хроматографического образца для идентификации его как препарата, являющегося целью выделения, с уменьшенным НСР, где указанный белок, являющийся целью выделения, представляет собой антитело или его антигенсвязывающую часть. Изобретение расширяет арсенал средств для очистки белков. 8 з.п. ф-лы, 41 ил., 4 табл., 11 пр.

РАДИАЛЬНАЯ ХРОМАТОГРАФИЯ ДЛЯ ВЫДЕЛЕНИЯ УГЛЕВОДОВ

Method and apparatus for reaction chromatography

Chromatographic process for the production of highly purified polyunsaturated fatty acids

Apparatus for purifying a liquid comprising a target substance

PROCEDURE FOR VOLUMES WITH HIGH THROUGHPUT WITH THE CRACKING OF BIO MOLECULES BY CHROMATOGRAPHI SYSTEMS

Multi-step separation process

MULTI-STEP SEPARATION PROCESS The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which comprises: (a) purifying the feed mixture in a first chromatographic separation step using as eluent a mixture of water and a first organic solvent, to obtain an intermediate product; and (b) purifying the intermediate product in a second chromatographic separation step using as eluent a mixture of water and a second organic solvent, to obtain the PUFA product, wherein the second organic solvent is different from the first organic solvent and has a polarity index which differs from the polarity index of the first organic solvent by between 0.1 and 2.0, wherein the PUFA product is other than alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), linoleic acid, an ALA mono- di- or triglyceride, a GLA mono- di- or triglyceride, a linoleic acid mono, di- or triglyceride, an ALA C1-C4 alkyl ester, a GLA C1-C4 ...

Sterile chromatography resin and use thereof in manufacturing processes

Provided herein are methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and at least one chelator and/or antioxidant agent, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified ...

CHROMATOGRAPHIC PURIFICATION OF POLYUNSATURATED FATTY ACIDS

... ²The present invention provides a chromatographic separation process for ²recovering a ²polyunsaturated fatty acid (PUFA) product from a feed mixture, which ²comprises: (a) purifying ²the feed mixture in a first chromatographic separation step using as eluent a ²mixture of water ²and a first organic solvent, to obtain an intermediate product; and (b) ²purifying the intermediate ²product in a second chromatographic separation step using as eluent a mixture ²of water and a ²second organic solvent, to obtain the PUFA product, wherein the second organic ²solvent is ²different from the first organic solvent and has a polarity index which ²differs from the polarity ²index of the first organic solvent by between 0.1 and 2.0, wherein the PUFA ²product is other ²than alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), linoleic acid, an ²ALA mono- di- or ²triglyceride, a GLA mono- di- or triglyceride, a linoleic acid mono, di- or ²triglyceride, an ALA C1-C4²alkyl ester, a GLA C1-C4 alkyl ester ...

SMB PROCESS FOR PRODUCING HIGHLY PURE EPA FROM FISH OIL

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture which is a fish oil or which is derived from fish oil, which process comprises the steps of: (i) purifying the feed mixture in a chromatographic separation step, to obtain a first intermediate product; and (ii) purifying the first intermediate product obtained in (i) in a simulated or actual moving bed chromatographic separation step, to obtain a second intermediate product; and (iii) purifying the second intermediate product obtained in (ii) in a simulated or actual moving bed chromatographic separation step, to obtain the PUFA product; wherein an aqueous organic solvent is used as eluent in each separation step; saturated and/or monounsaturated fatty acids present in the feed mixture are removed in the first separation step; the PUFA product is separated from different components of the feed mixture in steps (ii) and (iii); and the PUFA product ...

SEPARATION PROCESS

The present invention relates to a method of separating betaine and at least one other component from a sugar beet based fermentation solution. The invention is based on the use of a combination of SAC resins and WAC resins in a specific order and in specified proportions in a chromatographic SMB separation system. The chromatographic separation system is preferably a single integrated SMB system comprising both SAC and WAC resin beds.

Selective sepn chromatographic process

APPARATUS AND METHOD FOR REMOVING SOLUBLE SUBSTANCES DISSOLVED IN AQUEOUS SOLUTION

La présente invention concerne un dispositif (2) d'extraction de substances solubles dissoutes dans une solution aqueuse, le dispositif d'extraction comprenant une paroi périphérique (7) et un agent adsorbant contenu dans cette paroi périphérique, l'agent adsorbant étant apte à extraire au moins une partie des substances solubles par contact avec la solution aqueuse. Selon l'invention, le dispositif d'extraction (2) comprend en outre un labyrinthe (9), délimitant un chemin de circulation (A) de la solution aqueuse au sein de la paroi périphérique (7), le labyrinthe comprenant une entrée principale (4) et une sortie principale (5) de solution aqueuse reliées fluidiquement entre elles par le chemin de circulation, l'agent adsorbant étant disposé au sein du labyrinthe (9) de manière à être mis en contact avec la solution aqueuse lors de la circulation de cette dernière le long du chemin de circulation.

Multi column chromatography system

The present invention relates to a method and apparatus for chromatographically analyzing each of a plurality of samples in detector, comprising an autosampler to contain a plurality of samples for chromatographic analysis and a pluraltity of chromatographic systems, each system comprising one or more pumps and one or more chromatographic columns. A detector is included for detecting compounds in the samples from each of the chromatography systems along with a valve positioned between the detector and the plurality of chromatography systems, the valve permitting each sample to reach the detector in sequence. A computer control device is included which adjusts the introduction of samples from the autosampler into the plurality of chromatography systems as well as the position of the valve to sequentially separate and deliver compounds within the samples to the detector.

Alternating flow column chromatography apparatus and method of use

An alternating flow column chromatography apparatus comprising a ‘U’ shaped or T shaped separation column including at least one loading port for loading of components for separation, a first purification column in fluid communication with one end of the separation column and a second purification column in fluid communication with another end of the separation column, at least one eluent input port, an eluate output port and an alternating flow valve in fluid communication with the primary eluent input port, the eluate output port, the first purification column and the second purification column wherein, when operated, the alternating flow valve reverses the flow of eluent through the purification columns and the separation column. Also a method of using the apparatus. A benefit of the apparatus and method is more efficient operation compared to existing direct flow column chromatography apparatuses.

PURIFICATION OF ANTIBODIES USING SIMULATED MOVING BED CHROMATOGRAPHY

The present invention relates to compositions and methods for the chromatographic purification of antibodies, such as monoclonal antibodies, employing improved simulated moving bed separation strategies and, in certain embodiments, Raman spectroscopy.

SYSTEM AND METHOD FOR SOLVENT MIXING IN A CHROMATOGRAPHY SYSTEM

A solvent mixing system includes a mixing tee, a centrifugal mixing path, and a low frequency blending mixer. The mixing tee has at least two solvent input ports and a solvent output port in fluid communication with one another. The centrifugal mixing path has a mixing path inlet in fluid communication with the solvent output port of the mixing tee. The centrifugal mixing path includes at least one coiled segment between the mixing path inlet and a mixing path outlet. The low frequency blending mixer is in fluid communication with the outlet of the centrifugal mixing path.

TWO-DIMENSIONAL FLUID SEPARATION WITH CONTROLLED PRESSURE

A sample separation apparatus (200) for separating a fluidic sample, the sample separation apparatus (200) comprising a first separation unit (204) for separating the fluidic sample, a first fluid drive (202) configured for conducting the fluidic sample to be separated through the first separation unit (204), a second separation unit (208), arranged downstream of the first separation unit (204), for further separating the fluidic sample after treatment by the first separation unit (204), a second fluid drive (206) configured for at least partially conducting the fluidic sample, after treatment by the first separation unit (204), through the second separation unit (208), and a fluidic valve (218) having fluidic interfaces (222, 224, 226, 228) fluidically coupled to the first fluid drive (202) and the second fluid drive (206) and being switchable for performing the separation of the fluidic sample, wherein the sample separation apparatus (200) is configured for adjusting a pressure at a predefined ...

METHOD FOR CONTROLLING IMPURITY OF CYCLOSPORIN IN AN EYE GEL

СПОСОБ КОНТРОЛЯ, ОЦЕНКИ И РЕГУЛИРОВАНИЯ ЦИКЛИЧЕСКОГО ХРОМАТОГРАФИЧЕСКОГО ПРОЦЕССА ОЧИСТКИ

Настоящее изобретение относится к способам контроля, оценки и регулирования циклических хроматографических процессов очистки. Предложен способ контроля, оценки и регулирования циклического хроматографического процесса очистки, включающего по меньшей мере два адсорбера, причем способ включает по меньшей мере следующие стадии: а) контроль хроматограммы, включающий измерение по меньшей мере одного текущего сигнала, пропорционального концентрации, в жидкости; b) оценка хроматограммы, включающая сравнение по меньшей мере одного из указанных текущих сигналов, пропорциональных концентрации, измеренных на стадии (а), с его пороговым значением; с) регулирование процесса хроматографической очистки посредством адаптации завершения текущей фазы на основании сравнения во время стадии (b) и начала следующей фазы. Причем последовательность стадий а)-с) выполняют в указанном порядке по меньшей мере два раза. В примесях присутствуют примеси, адсорбирующиеся слабее, чем продукт (W), и примеси, адсорбирующиеся ...

УСТРОЙСТВО ОЧИСТКИ ЖИДКОСТИ

Изобретение относится к устройствам очистки жидкости, преимущественно воды из локальных и/или муниципальных источников, для бытового и/или питьевого водоснабжения и предназначено для использования в бытовых условиях, на дачных и садовых участках. Устройство для очистки жидкости содержит корпус, включающий верхнюю и нижнюю зоны фильтрации с размещенной в них фильтрующей средой, средство изменения направления потока очищаемой жидкости, средство отвода воздуха, предназначенное для отвода воздуха из нижней зоны фильтрации и имеющее элемент для выхода воздуха, расположенный в верхней стенке средства отвода воздуха. Устройство выполнено с возможностью отвода воздуха из нижней зоны фильтрации с помощью восходящего потока очищаемой жидкости в начале процесса фильтрации во время заполнения устройства очищаемой жидкостью. Средство изменения направления потока очищаемой жидкости, содержащее фильтрующую среду, одновременно является нижней зоной фильтрации, сформировано камерой с фильтрующей средой ...

СИСТЕМА И СПОСОБ ИЗВЛЕЧЕНИЯ ПРОДУКТОВ С ИСПОЛЬЗОВАНИЕМ АДСОРБЦИИ В ИМИТИРОВАННОМ ДВИЖУЩЕМСЯ СЛОЕ

Изобретение относится к способу адсорбционного разделения компонента из потока, предпочтительно ароматических углеводородов. Поток исходного материала и поток десорбента вводят в два разных порта через две разные линии передачи вдоль камеры адсорбционного разделения с множеством слоев. Камера содержит заданное количество разнесенных друг от друга портов с соответствующими линиями передачи, сообщающимися по текучей среде друг с другом для подачи и удаления текучей среды в и из камеры адсорбционного разделения. Отбор потока экстракта и потока рафината осуществляют через две разные линии передачи. Проводят промывание остаточной текучей среды в промежуточной линии передачи зоны очистки между линией передачи потока исходного материала и линией передачи потока экстракта в направлении от камеры адсорбционного разделения для удаления по меньшей мере части остаточной текучей среды из промежуточной линии передачи. Направляют остаточную текучую среду, вымытую из промежуточной линии передачи, в другую ...

СПОСОБ ОЧИСТКИ УГЛЕВОДОРОДНОГО СЫРЬЯ

... 1. Способ очистки углеводородного сырья, содержащего примеси, в котором одновременно осуществляют следующие этапы:a) обработку в жидкой фазе углеводородного сырья в первой адсорбционной установке, содержащей первую и вторую адсорбционные колонны (1, 2), заполненные соответственно первым и вторым твердым адсорбентом, причем первая и вторая адсорбционные колонны (1, 2) работают параллельно и попеременно в режиме адсорбции и в режиме регенерации, причем упомянутое углеводородное сырье вводят в первую адсорбционную колонну (1) и приводят в контакт с первым твердым адсорбентом, и на выходе первой адсорбционной колонны (1) отбирают поток углеводородов, обедненный примесями;b) обработку вторичного жидкого углеводородного сырья, которое состоит или из фракции углеводородного сырья, или из фракции потока углеводородов, обедненного примесями, в установке обработки (3, 4, 22, 24), и отбор обработанного вторичного жидкого углеводородного сырья из указанной установки обработки;c) нагревание обработанного ...

МНОГОСТАДИЙНЫЙ СПОСОБ РАЗДЕЛЕНИЯ

МНОГОСТАДИЙНЫЙ СПОСОБ РАЗДЕЛЕНИЯ

Устройство для контактирования стационарного слоя адсорбента с жидкостью

A modified outlet end fitting and frit for reaction chromatography

A chromatography column having a fluid outlet (305) configured with two or more fluid ports (301, 302), of which are: one or more reactant ports connecting a reactant flow from a reactant source with the eluate flow, and one or more are product ports being in communication with one or more processing units to process the eluate flow. The ports may be positioned to react with a portion of the eluate flowing from restricted regions of the column to allow for different processing, specifically the central region (301) separately from its peripheral region (302). Processing units may be a detector, a fraction collector and/or another chromatography column. The column outlet may further comprise a split frit (303) for further segmentation of the eluent. Also given is the method of reaction chromatography including the introduction and reaction of a reactant flow with the eluate flow at the outlet of the column.

Process for purifying monoclonal antibodies

FLOW DISTRIBUTION APPARATUS

Multi-step separation process

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which comprises: (a) purifying the feed mixture in a first chromatographic separation step using as eluent a mixture of water and a first organic solvent, to obtain an intermediate product; and (b) purifying the intermediate product in a second chromatographic separation step using as eluent a mixture of water and a second organic solvent, to obtain the PUFA product, wherein the second organic solvent is different from the first organic solvent and has a polarity index which differs from the polarity index of the first organic solvent by between 0.1 and 2.0, wherein the PUFA product is other than alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), linoleic acid, an ALA mono- di- or triglyceride, a GLA mono- di- or triglyceride, a linoleic acid mono, di- or triglyceride, an ALA C ...

SYSTEM AND METHOD OF APPLIED RADIAL TECHNOLOGY CHROMATOGRAPHY

A system and method of applied radial technology chromatography using a plurality of beads is disclosed, with each bead comprising one or more pores therein having a diameter of about 250Å to about 5000Å, and each bead having an average radius between about 100 µm to about 250 µm. Also disclosed are processes for selecting beads for use in a radial flow chromatography column, and for purifying an unclarified feed stream using a radial flow chromatography column.

MULTI-STAGE SEPARATION DEVICE FOR USE WITH FLOWABLE SYSTEM OF SUBSTANCES

A multi-stage separation device for separating a first fluid from at least one other second substance, the first fluid and second substance forming a flowable system of substances. The device comprising a housing having a substantially cylindrical form about a central axis with a wall disposed between a first end and second end, and an inlet disposed near said first end of the housing and an outlet in the second end. The wall when viewed in cross section perpendicular to the central axis has an ever decreasing radius spiralling between at least a first edge of said wall and a second edge of the wall. The first edge and second edge form part of the periphery of an inlet in the housing. At least one permeable cylindrical separation module is disposed within the housing.

HEATED CHROMATOGRAPHIC SEPARATION PROCESS

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which process comprises passing the feed mixture through one or more chromatographic columns containing, as eluent, an aqueous organic solvent, wherein the temperature of at least one of the chromatographic columns through which the feed mixture is passed is greater than room temperature.

PROCESS Of EXTRACTION Of a PRODUCT BY CENTRIFUGATION

PROCEDE ET DISPOSITIF DE CENTRIFUGATION POUR TRAITER DES PRODUITS FLUIDES

L'invention se rapporte au traitement de produits fluides par centrifugation. Elle concerne un dispositif centrifuge comprenant un rotor, une première série de dispositifs tubulaires 60 disposés autour de ce rotor, chaque dispositif formant une premiere voie d'écoulement de fluide, une source de fluides de traitement, un distributeur dans le rotor pour diriger ces fluides de traitement dans les premières voies d'écoulement de fluide, caractérisé en ce qu'il comprend une seconde série de paires de dispositifs tubulaires 62 disposés autour du rotor, formant chacun une seconde voie d'écoulement de fluide, radialement à l'extérieur de la première série de dispositifs, et des moyens d'entraînement pour accélérer sélectivement le rotor dans un premier sens et dans un second sens pour établir entre les dispositifs tubulaires des voies d'écoulement de fluide non reliées à partir du distributeur à travers un dispositif de la première série et un membre correspondant d'une paire de dispositifs de ...

Method for obtaining uniform stream in adsorption column

A method for obtaining uniform stream in an adsorption column is disclosed. This method uses an adsorption column having at least one single-flow passage in which the ratio of the inner diameter of the column to the average particle size of a packing to be packed in the adsorption column is at least 20, and comprises classifying the packing to be packed in the adsorption column and packing the fractions of the packing into the adsorption column in the order of particle size. Thus, a desired substance or substances contained in a mixture can be adsorbed and then separated as a uniform stream in an adsorption column.

Device and method for extracting soluble substances dissolved in an aqueous solution

A device for extracting soluble substances dissolved in an aqueous solution, the extraction device including a peripheral wall and an adsorbent agent contained in the peripheral wall, the adsorbent agent being capable of extracting at least one portion of the soluble substances by contact with the aqueous solution, the extraction device also including a maze, defining a circulation path for the aqueous solution within the peripheral wall, the maze including a main inlet and a main outlet of aqueous solution, in fluid communication with one another via the circulation path, the adsorbent agent being arranged inside the maze so as to be placed in contact with the aqueous solution during the circulation of the latter along the circulation path.

MULTI-STEP SEPERATION PROCESS

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which comprises: (a) purifying the feed mixture in a first chromatographic separation step using an eluent a mixture of water and a first organic solvent, to obtain an intermediate product; and (b) purifying the intermediate product in a second chromatographic separation step using as eluent a mixture of water and a second organic solvent, to obtain the PUFA product, wherein the second organic solvent is different from the first organic solvent and has a polarity index which differs from the polarity index of the first organic solvent by between 0.1 and 2.0, wherein the PUFA product is other than alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), linoleic acid, an ALA mono- di- or triglyceride, a GLA mono- di- or triglyceride, a linoleic acid mono- di- or triglyceride, an ALA C1-C4 alkyl ester, a GLA C1-C4 alkyl ester or a linoleic acid ...

Multi column chromatography system

The present invention relates to a method and apparatus for chromatographically analyzing each of a plurality of samples in detector, comprising an autosampler to contain a plurality of samples for chromatographic analysis and a plurality of chromatographic systems, each system comprising one or more pumps and one or more chromatography columns. A detector is included for detecting compounds in the samples from each of the chromatography systems along with a valve positioned between the detector and the plurality of chromatography systems, the valve permitting each sample to reach the detector in sequence. A computer control device is included which adjusts the introduction of samples from the autosampler into the plurality of chromatography systems as well as the position of the valve to sequentially separate and deliver compounds within the samples to the detector.

Temperature-assisted on-column focusing

A method of comprising: introducing a sample volume into an inlet end of a liquid chromatography column, wherein the liquid chromatography column includes a focusing segment proximal to the inlet end of the liquid chromatography column and a separation segment proximal to an elute outlet of the liquid chromatography column; maintaining only the focusing segment at a first temperature as the sample is introduced into the focusing segment; and subsequently heating the focusing segment to a second temperature that is higher than the first temperature after the entire sample volume has been introduced into the focusing segment.

A TURBULENT FLOW MIXING DEVICE FOR USE IN A CHROMATOGRAPHY SYSTEM

ПЕРФУЗИОННЫЙ БИОРЕАКТОР С СИСТЕМАМИ ФИЛЬТРАЦИИ

Группа изобретений относится к перфузионному устройству для культивирования клеток и к способу культивирования клеток. Перфузионное устройство содержит: сосуд биореактора, первый фильтрационный узел, второй фильтрационный узел, выполненный с возможностью эксплуатации параллельно с первым фильтрационным узлом, и устройство управления. Первый фильтрационный узел содержит: первую систему фильтрации; насос для сбора, последовательно соединенный с первой системой фильтрации, и датчик. Второй фильтрационный узел содержит: вторую систему фильтрации и второй насос для сбора, последовательно соединенный со второй системой фильтрации. Устройство управления осуществляет прием информации от датчика о работоспособном состоянии первой системы фильтрации и в ответ на определение того, что первая система фильтрации находится в неработоспособном состоянии, инициирует прекращение перекачивания первым насосом для сбора жидкой питательной среды через первую систему фильтрации. Осуществление группы изобретений ...

СПОСОБ ОЧИСТКИ УГЛЕВОДОРОДНОГО СЫРЬЯ

Изобретение относится к способу очистки углеводородного сырья, содержащего примеси, в котором одновременно осуществляют следующие этапы: a) обработку в жидкой фазе углеводородного сырья в первой адсорбционной установке, содержащей первую и вторую адсорбционные колонны (1, 2), заполненные соответственно первым и вторым твердым адсорбентом, причем первая и вторая адсорбционные колонны (1, 2) работают параллельно и попеременно в режиме адсорбции и в режиме регенерации, причем упомянутое углеводородное сырье вводят в первую адсорбционную колонну (1) и приводят в контакт с первым твердым адсорбентом, и на выходе первой адсорбционной колонны (1) отбирают поток углеводородов, обедненный примесями; b) обработку вторичного жидкого углеводородного сырья, которое состоит или из фракции углеводородного сырья, или из фракции потока углеводородов, обедненного примесями, в установке обработки (3, 4, 22, 24), и отбор обработанного вторичного жидкого углеводородного сырья из указанной установки обработки ...

СПОСОБЫ ЗАХВАТА ПРЕДСТАВЛЯЮЩИХ ИНТЕРЕС ЧАСТИЦ ИЗ СМЕСИ

... 1. Способ захвата представляющих интерес вирусоподобных частиц из смеси, включающий использование расширяющегося слоя адсорбента; где указанный способ включает этапы (a) предоставления расширяющегося слоя адсорбента; (b) приведения смеси, содержащей материалы разрушенных клеток-хозяев, в контакт с адсорбентом так, что вирусоподобные частицы в смеси связываются с адсорбентом; (c) необязательной отмывки адсорбента и (d) необязательной элюции вирусоподобных частиц из адсорбента.2. Способ по п. 1, где вирусоподобные частицы содержат белок вируса гриппа.3. Способ по п. 2, где вирусоподобные частицы содержат гемагглютинин; соответственно подтип гемагглютинина, выбранный из группы, состоящей из H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15 или H16 или комбинации двух или более из них.4. Способ по п. 1, где смесь содержит разрушенные растительные клетки; соответственно где растение представляет собой растение табака, инфильтрированное молекулами нуклеиновой кислоты, транзиторно ...

Ein mikrofluidischer HPLC-Chip für Glykopeptid-Analyse mit integrierter HILIC-Anreicherung

Ein mikrofluidisches Gerät zur Glykopeptid-Analyse enthält eine Anreicherungssäule, die zum Binden von Kohlenhydraten in der Lage ist; eine Trapping-Säule, die zum Binden von Peptiden in der Lage ist; wobei die Trapping-Säule konfiguriert ist, stromabwärts der Anreicherungssäule angeschlossen zu werden; eine Trennsäule, wobei die Trennsäule konfiguriert ist, stromabwärts der Trapping-Säule angeschlossen zu werden; und eine Mehrzahl von Ports, die zum Zusammenarbeiten mit einem Schaltgerät konfiguriert sind, um eine Mehrzahl von Flusspfaden zu bilden, wobei einer der Mehrzahl von Flusspfaden es erlaubt, dass die Trapping-Säule in Fluidkommunikation mit der Trennsäule ist. Ein Verfahren zur Glykopeptid-Analyse unter Verwendung eines mikrofluidischen Geräts, aufweisend eine Trapping-Säule und eine Trennsäule, wobei das Verfahren aufweist: Applizieren einer Probe von Peptiden an das mikrofluidische Gerät; Trappen der Peptide an der Trapping-Säule; Eluieren der Peptide von der Trapping-Säule ...

Chromatography columns,systems and methods

Continuous moving bed chromatography

A method and apparatus for use in continuous chromatography, the apparatus comprises a column made of a material with magnetic permeability, a bed containing magnetic materials that are free to move inside the column, and a device located outside the column generating magnetic fields which move the bed. In use a buffer solution, a feed solution containing a target substance and a solution containing the magnetic materials may enter the column through dedicated apertures. The feed solution without the target substance may flow out of the column at one end and magnetic materials bound with the target substance flow out at another end of the column. The apparatus enables continuous counter-current moving bed chromatography.

Process for purifying target substances

Method and apparatus for reaction chromatography

Methods and compositions for chromatography

Improved liquid handling for filtration and preparative chromatography

Purification of antibodies using simulated moving bed chromatography

The present invention relates to compositions and methods for the chromatographic purification of antibodies, such as monoclonal antibodies, employing improved simulated moving bed separation strategies and, in certain embodiments, Raman spectroscopy.

CENTRIFUGAL METHOD AND APPARATUS FOR PROCESSING FLUID MATERIALS

CENTRIFUGAL CHROMATOGRAPHY APPARATUS AND SYSTEM

MULTI COLUMN CHROMATOGRAPHY SYSTEM

The present invention relates to a method and apparatus for chromatographically analyzing each of a plurality of samples in detector, comprising an autosampler to contain a plurality of samples for chromatographic analysis and a plurality of chromatographic systems, each system comprising one or more pumps and one or more chromatography columns. A detector is included for detecting compounds in the samples from each of the chromatography systems along with a valve positioned between the detector and the plurality of chromatography systems, the valve permitting each sample to reach the detector in sequence. A computer control device is included which adjusts the introduction of samples from the autosampler into the plurality of chromatography systems as well as the position of the valve to sequentially separate and deliver compounds within the samples to the detector.

IMPROVED LIQUID HANDLING FOR FILTRATION AND PREPARATIVE CHROMATOGRAPHY

A method and system are provided for high-precision separation of pharmaceutical or biotechnology liquids. The separation can be in accordance with direct flow filtration, tangential flow filtration or preparative chromatography. Movement of the pharmaceutical or biotechnology liquid within a flow path is controlled according to a selected pattern. Selected patterns include automatically and progressively increasing the flow rate within the unit, automatically and progressively increasing the pressure within the unit or initially proceeding according to a relatively high constant flow rate and switching to a relatively high constant pressure at a time when a given parameter is attained.

COLUMN GEOMETRY TO MAXIMIZE ELUTION EFFICIENCIES FOR MOLYBDENUM-99

At least one system for eluting a radioactive material and a method of eluting a radioactive material is provided. The system for eluting a radioactive material may include an elution column configured to enclose an radioactive material, a first sealing member sealing a first end of the elution column, a second sealing member sealing a second end of the elution column, an elution supply source connected to the first end of the elution column via a first needle, a collection system connected to the second end of the elution column via a second needle, and a filter in the elution column, the filter being configured to support the radioactive material and prevent the radioactive material from contacting the second needle.

PROCESS AND CHROMATOGRAPHIC SEPARATION DEVICE OF FRACTIONS Of a MIXTURE

L'invention se rapporte à un procédé de séparation de fractions d'un mélange à séparer, dans un dispositif (1) présentant : - plusieurs colonnes (21, 22, 23, ...) de chromatographie montées en série, - une boucle de séparation ouverte et comprenant en entrée un point d'injection (6) d'éluant dans une des colonnes et en sortie un point de soutirage (8) de fraction du mélange , le procédé comprenant les étapes de : - injection discontinue du mélange à séparer dans la boucle de séparation (4) ouverte, - collecte d'au moins deux fractions, - décalage d'au moins une colonne des points d'injection d'éluant et de soutirage de fraction (6, 8) de la boucle de séparation (4). L'invention se rapporte aussi à un dispositif de séparation de fractions d'un mélange à séparer.

Column geometry to maximize elution efficiencies for molybdenum-99

At least one system for eluting a radioactive material and a method of eluting a radioactive material is provided. The system for eluting a radioactive material may include an elution column configured to enclose an radioactive material, a first sealing member sealing a first end of the elution column, a second sealing member sealing a second end of the elution column, an elution supply source connected to the first end of the elution column via a first needle, a collection system connected to the second end of the elution column via a second needle, and a filter in the elution column, the filter being configured to support the radioactive material and prevent the radioactive material from contacting the second needle.

METHOD AND UNIT FOR SEQUENTIAL CENTRIFUGAL STRATIFICATION CHROMATOGRAPHY

The method and plant disclosed combine the advantages of the preparatory centrifugal stratification chromatography, of the analytical horizontal, anticircular and sequential thin layer chromatography. In this new on-line preparatory method the addition of solvent may be varied in parallel, locally and temporally. It is thus possible to improve sections of the integral chromatogram in their separation by selecting for these partial problems the optimum composition of the solvent. The separations are basically obtained in two different ways: 1. The circular separations are effected by means of the centrifugal force by using a sequential solvent supply (20), the separation taking place from the inside to the outside. The separated substances may be brought back to the inner part of the layer by recycling. 2. The anticircular separations are performed by means of the capillary force by using an annular-shaped solvent supply (19), the substance zones being concentrated and/or developed from ...

Liquid chromatograph

A liquid chromatograph has a mobile phase delivery section including, between mobile phase channels connected respectively to a plurality of mobile phase containers and a delivery pump, channel switching valves for selecting one of the mobile phase channels. This liquid chromatograph whose channel switching valves have a hierarchical structure including at least two stages includes a valve connection state setting section that is configured to set the connection state between the channel switching valves and the connection state between the channel switching valves and the mobile phase channels, and further includes a per channel total delivery amount calculation section that is configured to calculate the total delivery amount of mobile phase per mobile phase channel based on a connection state according to the valve connection state setting section, the delivery amount per unit time by the delivery pump, and the operation time of the delivery pump.

REACTOR FOR BIOLOGICAL OR CHEMICAL TRANSFORMATION

СТЕРИЛЬНАЯ ХРОМАТОГРАФИЧЕСКАЯ СМОЛА И ЕЕ ПРИМЕНЕНИЕ В СПОСОБАХ ПРОИЗВОДСТВА

Изобретение относится к способу снижения бионагрузки аффинной хроматографической смолы, включающему подвергание контейнера, включающего композицию, содержащую аффинную хроматографическую смолу и по меньшей мере один антиоксидант, воздействию дозы гамма-излучения между приблизительно 15 кГр и приблизительно 45 кГр, где по меньшей мере один антиоксидант присутствует в количестве, достаточном для снижения утраты связывающей способности хроматографической смолы после воздействия дозы гамма-излучения. Также настоящее изобретение относится к композиции, включающей хроматографическую смолу и по меньшей мере один антиоксидант. 2 н. и 34 з.п. ф-лы, 11 ил., 4 табл., 5 пр.

ОЧИСТКА АНТИТЕЛ С ПОМОЩЬЮ ХРОМАТОГРАФИИ С ПСЕВДОДВИЖУЩИМСЯ СЛОЕМ

... 1. Способ получения препарата белка, являющегося целью выделения, с уменьшенным содержанием белка клетки-хозяина (HCP) из образца смеси, содержащей белок, являющийся целью выделения, и, по меньшей мере, один HCP, где указанный способ содержит:(a) проведение анализа Рамановской спектроскопии указанного образца смеси;(b) приведение указанного образца смеси в контакт с хроматографической смолой так, что смола нагружена до приблизительно 50-100% насыщения ее связующей способности; и(с) сбор хроматографического образца; и(d) проведение анализа Рамановской спектроскопии указанного хроматографического образца для идентификации его как препарата, являющегося целью выделения, с уменьшенным HCP.2. Способ по п.1, где хроматографическая смола выбрана из группы, состоящей из аффинной хроматографической смолы, ионообменной хроматографической смолы и хроматографической смолы гидрофобного взаимодействия.3. Способ по п.1, где белок, являющийся целью выделения, выбран из группы, состоящей из: ферментов; ...

СТЕРИЛЬНАЯ ХРОМАТОГРАФИЧЕСКАЯ СМОЛА И ЕЕ ПРИМЕНЕНИЕ В СПОСОБАХ ПРОИЗВОДСТВА

СТЕРИЛЬНАЯ ХРОМАТОГРАФИЧЕСКАЯ СМОЛА И ЕЕ ПРИМЕНЕНИЕ В СПОСОБАХ ПРОИЗВОДСТВА

Method and installation for regulating the modifier level in chromatography or supercritical extraction with recycling

A chromatography or supercritical extraction method is disclosed, in which the eluent comprises a mixture of a fluid and a modifier and in which the fluid is recycled. One exemplary method comprises an operation consisting in determining at least one quantity linked to the level of modifier that is mixed with the recycled fluid and, if necessary, a correction operation in order to limit variations in the level of modifier in the eluent at the inlet of the column or the extractor. The disclosure also relates to a chromatography or extraction installation.

Media For Membrane Ion Exchange Chromatography Based On Polymeric Primary Amines, Sorption Device Containing That Media, And Chromatography Scheme And Purification Method Using The Same

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (e.g., Protein A or Protein G) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

Elimination of Residual Transfer Line Raffinate from Feed to Increase Normal Paraffin Separation Unit Capacity

A process to increase the capacity of the adsorbent in a normal paraffin adsorption separation system is presented. A tertiary flush stream is used to improve the capacity of the simulated moving bed system by flushing residual raffinate from the feed transfer line. The flushing removes residual raffinate containing desorbent that competes with the adsorption of normal paraffins from the feedstream. The flush stream is a material that will displace fluid in the column, but will not enter the pores of the adsorbent.

Chromatography of polyolefin polymers

The invention provides a method for one-dimensional chromatography of a polyolefin polymer, comprising introducing a solution of the polyolefin polymer into a liquid flowing through a liquid chromatography stationary phase, the liquid chromatography stationary phase comprising graphitic carbon, and wherein the polyolefin polymer emerging from the liquid chromatography stationary phase has a retention factor greater than zero, and wherein the solution introduced into the liquid chromatography stationary phase is subjected to a temperature gradient, and/or the solution is subjected to a solvent gradient. The invention also provides a method for multi-dimensional chromatography of a polyolefin polymer, comprising introducing a solution of the polyolefin polymer into a liquid flowing through a first liquid chromatography stationary phase or a field flow fractionation device, and subsequently flowing the solution through a second liquid chromatography stationary phase, the second liquid chromatography stationary phase comprising graphitic carbon, and wherein the polyolefin polymer emerging from the liquid chromatography stationary phase has a retention factor greater than zero. The invention also provides an apparatus for polyolefin polymer chromatography, comprising a liquid chromatography stationary phase, the liquid chromatography stationary phase comprising graphitic carbon and at least one inert filler.

Product recovery from adsorption-separation purge fluids

Purge fluid from a vessel head in an adsorption process is distributed to recovery processes according to the purity of product contained in the fluid. Extract-rich fluid thus is routed directly to recovery of the extract product. Distribution preferably is determined by internal positioning of feed, desorbent and product streams in the adsorption vessel.

Fractionation of de-asphalted oil of vacuum resid using preparative high performance liquid chromatographic separations

Two quantitative separation approaches to fractionate de-asphalted oils (DAOs) into seven classes of compounds (saturates, 1-4 + ring-aromatics, sulfides, and polars). In the first step (named as “SGS”) of present invention, the DAO of a petroleum vacuum resid is separated in to four classes of compounds, namely saturates, aromatics, and sulfides. In this first step of separation, about 3 grams of a DAO can be separated. Whereas in the second step (named as “ARC” separation) of invention, only less than 300 mg of the aromatic fraction obtained in “SGS” (described above) can be further fractionated at very low temperature (about −40 degrees centigrade) into 4 fractions, namely 1-ring, 2-ring, 3-ring, and 4 + -ring aromatics. The present invention protocol is suitable for a wide range of compositionally different DAOs of petroleum vacuum resids.

Process for separation by selective adsorption on a solid containing a zeolite with a crystalline structure analogous to im-12

A process for adsorption separation uses a solid IM-12 type adsorbent to separate a molecular species from any feed.

Purification of Metals

A solid composition comprises: MnO 2 ; and a compound represented by the general formula (I) wherein: R is a polymer; each Y is independently a hydrogen or a negative charge; Z is either hydrogen or is not present; each n is independently 1, 2, 3, 4, 5 or 6; wherein the MnO 2 is bound to the compound of formula (I) so as to coat the surface thereof. Such a composition may be used for the separation of polyvalent metal species, such as Mo, from one or more accompanying impurities.

SEPARATING COMPONENTS OF MIXED FLUID USING A FUNCTIONALLY GRADED MATERIAL

A system for separating components of a fluid containing at least a first component and a second component includes a device having an inlet for introducing the fluid into the device, a first outlet for directing the first component of the fluid from the device, and a second outlet for directing the second component of the fluid from the device. A material that has a gradient in properties is located in the device between the inlet and the first and second outlets. The material has a first portion with an affinity for the first fluid component and a second portion with an affinity for the second fluid component. The first portion is positioned with relation to the first outlet such that the first component is directed from said device through the first outlet. The second portion is positioned with relation to the second outlet such that the second component is directed from the device through the second outlet. 1. An apparatus for separating components of a fluid containing at least a first component and a second component , comprising:a device;an inlet in said device for introducing the fluid into said device;a first outlet in said device;a second outlet in said device; anda material in said device between said inlet and said first and second outlets that has a gradient in properties, said material having a first portion with an affinity for the first component of the fluid and a second portion with an affinity for the second component of the fluid;wherein said first portion of said material has a location with relation to said first outlet such that the first component of the fluid is directed from said device through said first outlet, andwherein said second portion of said material has a location with relation to said second outlet such that the second component of the fluid is directed from said device through said second outlet.2. The apparatus for separating components of a fluid of claim 1 , wherein said fluid has a direction of flow between said inlet and ...

AN APPARATUS FOR CONTINUOUS SEPARATION OF VALINE AND A METHOD FOR CONTINUOUS SEPARATION OF VALINE USING THE SAME

The present invention relates to an apparatus for continuous separation of valine from the mixture comprising amino acids such as leucine, isoleucine, etc. and a method for continuous separation of valine by using the same, and the present invention can continuously separate valine from the mixture comprising amino acids such as leucine, isoleucine, etc. in a high purity and yield. 1. An apparatus for continuous separation of valine , which comprising:a Desorbent port (D);a Feed port (F);a Raffinate port (R);an Extract port (E);{'b': 10', '20', '30, 'a number of rotary valves (, , ) each selectively connected to the ports (D, F, R, E); and'}{'b': 40', '50', '60', '10', '20', '30, 'a number of chromatography zones (, , ) each equipped with every a number of rotary valves (, , ),'}{'b': 10', '20', '30, 'wherein the number of rotary valves (, , ) are connected to each other,'}{'b': 10', '20', '30', '10', '10', '10', '20', '20', '20', '30', '30', '30, 'i': a,', 'b,', 'c', 'a,', 'b,', 'c', 'a,', 'b,', 'c, 'wherein the number of rotary valves (, , ) are equipped with the number of connection ports () () (), respectively, and'}{'b': 10', '10', '10', '20', '20', '20', '30', '30', '30', '10', '20', '30', '10', '20', '30, 'i': a,', 'b,', 'c', 'a,', 'b,', 'c', 'a,', 'b,', 'c, 'wherein only any one of the number of connection ports () () () is opened, along with the rotation of the number of rotary valves (, , ), and subsequently any one of the rotary valves (, , ) selectively connected to each of the ports (D, F, R, E) are changed.'}2. The apparatus for continuous separation of valine according to claim 1 , wherein:{'b': 10', '20', '30, 'the number of rotary valves (, , ) rotate after a short period of time, and'}{'b': 10', '20', '30', '10', '20', '30, 'the rotary valves (, , ) connected to the ports (D, F, R, E) are changed, along with the rotation of the number of rotary valves (, , ).'}3. The apparatus for continuous separation of valine according to claim 2 , wherein:{'b': ...

SYSTEM AND PROCESS FOR BIOPOLYMER CHROMATOGRAPHY

A chromatography system for separation of a biopolymer is described, comprising at least one feed tank, at least one hold tank, at least one elution buffer tank, at least one eluate tank, at least two packed bed chromatography columns and for each packed bed chromatography column at least one pump and at least one outlet detector both connected to said each packed bed chromatography column, wherein the feed tank, the hold tank(s), the elution buffer tank and the eluate tank are each connected to the packed bed chromatography columns via a system of valves. 11344445678781011781213781478abc. A chromatography system () for separation of a biopolymer , comprising: at least one feed tank () , at least one hold tank (; , , ) , at least one elution buffer tank () , at least one eluate tank () , at least two packed bed chromatography columns ( , ) and for each column ( , ) at least one pump () and at least one outlet detector () both connected to said each column ( , ) , wherein said feed tank , hold tank(s) , elution buffer tank and eluate tank are each connected to said at least two columns via a system of valves () and wherein said hold tank(s) is/are connected to at least one inlet end () of a column ( , ) and at least one outlet end () of a column ( , ).2212. The chromatography system of claim 1 , further comprising at least one control unit () claim 1 , electrically claim 1 , pneumatically or hydraulically connected to said system of valves ().3151617. The chromatography system of claim 1 , further comprising at least one equilibration buffer tank () claim 1 , at least one wash buffer tank () and/or at least one regeneration liquid tank ().4444414781387abc. The chromatography system of claim 1 , wherein said at least one hold tank (; claim 1 , claim 1 , ) is adapted to receive a fluid from an outlet end () of one column ( claim 1 , ) and to convey a fluid to the inlet end () of another column ( claim 1 , ).578. The chromatography system of claim 1 , wherein said ...

Continuous processing methods for biological products

The present invention is directed to the development of continuous processing technology for the purification of biopharmaceuticals and biological products, such as monoclonal antibodies, protein therapeutics, and vaccines. Methods for continuous processing of a biological product in a feed stream toward formulation of a purified bulk product are described.

METHODS AND COMPOSITIONS FOR CHROMATOGRAPHY

The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need for support structures. Chromatography separation techniques, including but not limited to, ion exchange, size separation, affinity chromatography, ion exclusion, ligand exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. Such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications. 1. An apparatus for isolating a target molecule , comprising:at least one rotating chamber with an inlet port and an outlet port, the chamber containing a plurality of chromatography particles in an interior thereof and rotating about a horizontal axis to create a centrifugal force on the particles; anda pressurized liquid source containing a heterogeneous liquid comprising the target molecule, the first pressurized liquid source in fluid communication with the inlet port and configured to flow the heterogeneous liquid through the inlet port to the interior of the chamber such that a liquid flow force is imparted on the chromatography particles inside the chamber, wherein the liquid flow force substantially opposes the centrifugal force, wherein a gravitational force contributes to a resultant vector summation of all forces acting on the chromatography particles, and wherein the gravitational, liquid flow, and centrifugal forces substantially immobilize the chromatography particles in a fluidized chromatography bed inside the chamber;wherein the outlet port is configured to discharge the heterogeneous liquid less the target molecule.2. The apparatus of claim 1 , wherein the chamber is configured to adjustably rotate to affect either the density or shape of the fluidized chromatography bed ...

Methods and compositions for deacidifying fruit juice

The present invention relates to a method for deacidifying fruit juice comprising the steps of providing a resin having a tertiary amine functionality and contacting the fruit juice with said resin to extract the uncharged free organic acids from said juice. Preferably, this is accomplished by using different column processes that are serially connected such that the substance or material exiting one column process is fed to another column process. The term “column process” is defined herein as the chemical or physical process that occurs in the

DISPOSABLE HORIZONTAL OR RADIAL FLOW TYPE CHROMATOGRAPHIC COLUMN

A liquid chromatography column, utilizing horizontal or radial flow of sample material passing there through, preferably in inward direction, comprising: a housing defining a chamber therein and including at least one removable end section, e.g. screw lid; a first and second longitudinally extending porous fits positioned within said chamber of said housing; a bed or packing of, preferably particulate, chromatographic separation material positioned within said chamber of said housing and intermediate said porous frits, the first of said porous frits being adjacent said housing and an inlet channel, the second of said porous frits being positioned adjacent a core member and an outlet channel; distribution means operatively connected to said inlet channel; collector means operatively connected to said outlet channel, said distribution means and said inlet channel being constructed to direct associated material to be separated in said bed evenly across a longitudinal length of said bed in a substantially horizontal direction. 17-. (canceled)8. A liquid chromatography column , utilizing radial flow of sample material passing there through in inward direction , comprising: a sealed housing defining a chamber therein and including opposite , said housing closing , longitudinal end sections of which at least one is removable and provided by a lid , and a cylindrical housing wall longitudinally extending between and connecting to said opposite end sections and closing the house; a first and a second longitudinally extending , cylindrical porous frit positioned within said chamber of said housing; a doughnut shaped packing of particulate chromatographic separation material contained in a longitudinally sealed doughnut shaped packing space positioned within said chamber of said housing and intermediate said porous frits , the first of said porous frits being adjacent said cylindrical housing wall and a sample material inlet channel of the housing , the second of said porous ...

Downstream bioprocessing device

Large-scale downstream processing of secreted recombinant proteins is provided in a single device, wherein the contents of a plurality of bioreactors are combined simultaneous to their harvesting and purification resulting in significant savings of time and the cost of manufacturing.

CAGED BAGS OF POROUS MATERIALS

Systems and methods employing beds of bagged and caged absorbent and adsorbent materials are disclosed. These inventions are useful in the area of solid phase extraction. 1. A caged porous bag comprising a bed of solid phase material within a porous meshed cloth bag , at least a first part of the bag being locked in a cage , wherein the bed has a minimum width that is defined as the minimum distance through the bed along a straight line that intersects at least one porous meshed side of the bed at a right angle and passes through a point at the center of mass of the bed , said straight line passing sequentially through a porous meshed side of the bag , the bed , and a second porous meshed side of the bag with said sides of the bag contacting the bed where said line passes through , and wherein the bed has a maximum length which is the distance between the two ends of the bed that are farthest apart , and the ratio of said length to the said width is at least 2.2. The caged porous bag of claim 1 , wherein the ratio of said length to said width of the bed is at least 5.3. The caged porous bag of claim 1 , wherein at least 90% of the volume of the meshed cloth bag is occupied by said bed.4. The caged porous bag of claim 1 , wherein at least one part of the meshed cloth bag is locked in contact with the cage by mechanical stress claim 1 , embedding or covalent binding.5. The caged porous bag of claim 1 , wherein the cage is external to the meshed cloth bag.6. The caged porous bag of claim 1 , wherein the cage comprises a container claim 1 , wherein at least part of the cage is in contact with the inside wall of the container.7. The caged porous bag of any of claim 1 , wherein the pores in the porous meshed cloth are selected from the range of about 1 to about 100 microns.8. The caged porous bag of claim 1 , wherein the cage comprises one or more rigid or semi-rigid meshes.9. The caged porous bag of claim 1 , wherein the cage closes an opening of the meshed cloth bag.10. ...

High purity chromatographic materials comprising an ionizable modifier

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.

Separation System

A process for separating a product from a multicomponent feedstream to an adsorption apparatus or system is described. The apparatus or system may comprise a moving-bed or a simulated moving-bed adsorption means. The product comprises at least one organic compound, such as an aryl compound with alkyl substitutes. In embodiments the conduits used to supply the feedstream to the apparatus or system are flushed with media of multiple grades. The improvement is more efficient use of the desorbent. In embodiments the process achieves improvements in one or more of efficiency of adsorption separation, capacity of adsorption apparatus systems, and purity of product attainable by adsorption process. 114-. (canceled)15201204205. A simulated moving bed adsorption apparatus , the improvement characterized by a fluid connection between the flush out conduit and the secondary flush input and/or , whereby primary flush out may be routed directly for use as secondary flush in , without being routed through intervening distillation apparatus. This application claims the benefit of U.S. Provisional Application No. 61/319,080, filed Mar. 30, 2010 and U.S. application Ser. No. 12/774,319, filed May 5, 2010, the disclosures of which are incorporated herein by reference in their entireties.The invention relates to a process for separating one or more of the components from two or more multicomponent fluid mixtures, and more particularly to a process for separating organic compounds from such a fluid mixture by means of adsorption apparatus, such as moving-bed or simulated moving-bed adsorption apparatus, and a system comprising such apparatus.Various means are currently available to separate the components of a multicomponent fluid mixture. If the densities of the components differ sufficiently, the effects of gravity over time may be adequate to separate the components. Depending on the quantities of the components involved, a centrifuge may be used to more rapidly separate components ...

CLASS OF HDAC INHIBITORS EXPANDS THE RENAL PROGENITOR CELLS POPULATION AND IMPROVES THE RATE OF RECOVERY FROM ACUTE KIDNEY INJURY

The invention concerns separation methods and systems including those comprising a continuous chromatographic simulated moving bed integrated with vapor compression distillation to create a high efficiency separations platform applicable to a broad range of separation functions. 1. A process for separation and purification of compounds in a liquid mixture with low energy input , the process comprising:passing a feed mixture comprising a first compound and a second compound into a simulated moving bed chromatography apparatus;the first compound acting as a solvent for the second compound which forms a precipitate at concentrations above a solubility limit;passing an eluent solvent into the simulated moving bed chromatography apparatus to separate the feed into a first stream and a second stream, wherein the first stream has an elevated concentration of the first compound in eluent and the second stream has an elevated concentration of the second compound in eluent, as compared to the feed;passing the first stream to a vapor compression distillation unit to generate a high purity stream of the first compound;vaporizing at least a portion of the eluent from the first stream at a first temperature to form a vapor, compressing the vapor to form an eluent condensate at a second temperature, such that the second temperature is greater than the first temperature, and the eluent condensate has a thermal energy content; andtransferring at least a portion of the thermal energy content of the eluent condensate into the first stream to be used in vaporizing the eluent in the first stream.2. The process of claim 1 , wherein less thermal energy is added to the process than would be needed to vaporize the high purity stream of the first compound.3. The process of further comprising:passing the second stream to a vapor compression distillation unit to recover additional eluent and to generate a concentrated second stream with less eluent.4. A process for separation and purification ...

Countercurrent Chromatography Rotor

A method for constructing a spiral tube support apparatus used in countercurrent chromatography, improvements to countercurrent chromatography tube support design, and methods of using the improved countercurrent chromatography apparatus are described. The spiral tube support apparatus may be constructed by a shape forming process such as a three dimensional printing process that in turn uses a laser sintering technique, and can be made out of any easily formed material. Shape changes on both the tube support and the top improve the performance of the tube support and ease of manufacturing. The improved tube support and new method of creation permit use in both micro or macro scale preparations and use in small or large molecule preparations. In particular, specific solvent systems are described that permit purification of proteins. 1. A method for manufacturing a countercurrent chromatography rotor comprising forming a first surface which comprises a plurality of interweaved spiral channels; one or more tubing access ports , and one or more curved radial channels , to obtain said rotor.2. The method of claim 1 , wherein said first surface is formed from one or more materials selected from the group consisting of a nylon polymer claim 1 , a plastic claim 1 , a polytetrafluoroethylene claim 1 , a polyvinyl chloride claim 1 , a polystyrene claim 1 , a polyamide PA-220 claim 1 , a photopolymer claim 1 , a FullCure® material claim 1 , a Polyjet 3D Printer® material claim 1 , a monomeric powder claim 1 , and a particulate comprising a metal or a metal composite.3. The method of claim 1 , wherein said forming is a process selected from the group consisting of laser sintering claim 1 , ultraviolet irradiation claim 1 , molding claim 1 , prototyping claim 1 , drilling and machining.4. The method of claim 1 , wherein said first surface further comprises a space in which weights may be placed claim 1 , a loop to hold tubing claim 1 , a retainer support claim 1 , a snap lock ...

Novel multimodal oscillatory chromatographic purification system

The present invention comprises a novel multimodal chromatography sequence of short length alternating adsorption and size exclusion media operating with gradient elution. The novel multimodal chromatography in an oscillating series utilizes the alternating solvent exchange media to reposition the active region of separation back in phase with the target solutes. Each solvent exchange column bed length in the sequence is designed to achieve a subtle decrease or increase in the solvent gradient (or salt gradient) concentration associated with the two solutes of interest which results in an extension of the active separation or increasing differences in solute velocity for two solutes of interest. The novel oscillatory chronographic system demonstrates much improved separation capability as shown by a one dimensional model.

A Method in Continuous Chromatography

The present invention relates to a method for purifying a target product in a flow-through chromatography system comprises at least a first column loaded with feed material from a feed source. The at least first column is purged after binding of impurities and wherein the outlet of purged material from the column is subsequently passed to the feed source. 1. A method for purifying a target product in a flow-through chromatography system comprising at least a first column loaded with feed material from a feed source , wherein the at least first column is purged after binding of impurities to produce purged material and wherein the purged material from the column is passed upstream of the at least one column to be re-purified.2. The method according to claim 1 , wherein said upstream passing of the purged material is passing to the feed source.3. The method according to further including the steps of:providing an outlet sensor arranged downstream of at least the first column;loading the first column with feed material from the feed source,detecting impurity breakthrough based on a signal detected by the outlet sensor of the first column,when a predetermined impurity breakthrough is detected, disconnecting the first column from the feed source,purging partly purified feed material from the first column using a purging buffer, andpassing the purged partly purified feed material to the feed source.4. The method according to claim 3 , wherein the or each outlet sensor is a UV sensor and the predetermined impurity breakthrough corresponds to a predetermined percentage of impurities in a target product downstream of the or each column.5. The method according to claim 4 , wherein the predetermined percentage of impurities in the target product is about 1% claim 4 , 10% claim 4 , 20% claim 4 , 50% or 70%.6. The method according to or claim 4 , wherein a UV sensor is provided upstream each column claim 4 , and the method further comprises dynamically controlling the impurity ...

Process for the Recovering of Paraxylene

Disclosed herein are processes for recovering paraxylene in which a first simulated moving bed adsorption unit is used to produce a paraxylene-rich extract stream that also contains a significant amount of the ethylbenzene and a paraxylene-depleted raffinate stream. Because a significant amount of the ethylbenzene is removed in the paraxylene-rich extract stream (at least enough to limit buildup in the isomerization loop), the paraxylene-depleted raffinate stream may be isomerized in the liquid phase. Avoiding vapor phase isomerization saves energy and capital, as liquid phase isomerization requires less energy and capital than the vapor phase isomerization process due to the requirement of vaporizing the paraxylene-depleted stream and the use of hydrogen, which requires an energy- and capital-intensive hydrogen recycle loop. 1. A process for recovering paraxylene , the process comprising:(a) introducing a hydrocarbon feed stream into a first simulated moving bed adsorption unit, wherein the hydrocarbon feed stream comprises a mixture of paraxylene (PX), metaxylene (MX), orthoxylene (OX), and ethylbenzene (EB);(b) introducing a desorbent stream into the first simulated moving bed adsorption unit, wherein the desorbent stream comprises desorbent;(c) withdrawing a first PX-rich extract stream from the first simulated moving bed adsorption unit, wherein the first PX-rich extract stream comprises desorbent, PX, and EB;(d) withdrawing a PX-depleted raffinate stream from the first simulated moving bed adsorption unit, wherein the PX-depleted raffinate stream comprises desorbent, MX, OX, and EB;(e) isomerizing at least a portion of the first PX-depleted raffinate stream at least partially in the liquid phase to produce an isomerized stream having a higher PX concentration than the first PX-depleted raffinate stream;(f) recycling at least a portion of the first isomerized stream to the first simulated moving bed adsorption unit; and(g) introducing the first PX-rich extract ...

VITAMIN E PRODUCTION METHOD AND VITAMIN E PRODUCTION DEVICE

A vitamin E production method and a vitamin E production device which can highly purify vitamin E in a vitamin E concentrated fraction are provided. A raw oil supply section supplies a raw oil to a series column in which two or more columns including a strongly basic anion exchanger are coupled in series to adsorb vitamin E included in the raw oil on the strongly basic anion exchanger of at least one column from among the series column. A desorption solution supply section supplies a desorption solution to a column on which vitamin E has been adsorbed to desorb vitamin E from the strongly basic anion exchanger of the column. 1. A vitamin E production method for recovering vitamin E included in a raw oil , the method having:an adsorption step of supplying the raw oil to a series column in which two or more columns comprising a strongly basic anion exchanger are coupled in series, thereby adsorbing vitamin E included in the raw oil on the strongly basic anion exchanger of at least one column from among the series column, anda desorption step of supplying a desorption solution to a column on which vitamin E has been adsorbed in the adsorption step, thereby desorbing the vitamin E from the strongly basic anion exchanger of the column.2. The vitamin E production method according to claim 1 , wherein in the desorption step claim 1 , the vitamin E is desorbed from the strongly basic anion exchanger of at least one column from among the series column claim 1 , except for a column through which the raw oil flows first.3. The vitamin E production method according to claim 1 , wherein in the desorption step claim 1 , the vitamin E is desorbed from the strongly basic anion exchanger of claim 1 , from among the series column claim 1 , at least a column through which the raw oil flows last.4. The vitamin E production method according to claim 1 , wherein in the series column claim 1 , the lengths of columns along a direction through which the raw oil flows are the same claim 1 , ...

SYSTEMS AND METHODS FOR PREPARING A POLYPEPTIDE FROM A MIXTURE

Embodiments of the present disclosure are directed to methods for preparing a target polypeptide from a mixture including the target polypeptide. The method may include contacting the mixture to a hydrophobic interaction chromatography (HIC) apparatus including multiple chromatographic zones. The method may further include passing the target polypeptide through the outlets of at least a first zone and a second zone of the HIC apparatus. A residence time for the mixture including the target polypeptide in a first zone may be approximately the same as a residence time of one or more mobile phases in the second zone. 1. A method for preparing a target polypeptide from a mixture including the target polypeptide , the method comprising:contacting the mixture including the target polypeptide to a first zone of a hydrophobic interaction chromatography (HIC) apparatus, the first zone having one or more chromatographic columns and an outlet;contacting one or more mobile phases to a second zone of the HIC apparatus, the second zone having one or more chromatographic columns and an outlet; andpassing the target polypeptide through the outlets of at least the first and second zones of the HIC apparatus;wherein a residence time for the mixture including the target polypeptide in the first zone is configured to be approximately the same as a residence time of the one or more mobile phases in the second zone.2. The method of claim 1 , wherein the target polypeptide is a monoclonal antibody.3. The method of claim 1 , wherein the one or more mobile phases comprises an equilibration buffer and a wash buffer.4. The method of claim 1 , further comprising passing an effluent including the target polypeptide from the first zone of the HIC apparatus to the second zone of the HIC apparatus.5. The method of claim 1 , wherein contacting the one or more mobile phases to the second zone of the HIC apparatus includes:contacting a wash buffer to the second zone of the HIC apparatus; and ...

LIQUID CHROMATOGRAPHY SYSTEMS

A liquid chromatographic (LC) system is introduced which comprises at least one fluidic stream, the fluidic stream comprising a sample-injection valve, a trap-bypass-selection valve, a column-bypass valve, a load-elute valve and a trap-selection valve. Also, a liquid chromatographic (LC) system is introduced which comprises at least one fluidic stream. The fluidic stream comprises a first substream and a second substream. The first substream comprises a first sample-injection valve, a load-elute valve and a trap-selection valve. The second substream comprises a second sample-injection valve and a column-bypass valve. The fluidic stream further comprises a trap-LC substream transfer valve and a substream-selection valve. The LC systems provide a broad choice of chromatographic options and modes and enable to flexibly and rapidly switch between them. 1. A liquid chromatographic (LC) system comprising at least one fluidic stream , the fluidic stream comprising:a sample-injection valve comprising a plurality of ports and a multi-way switch to switch fluidic connections between ports, including a sample input port fluidically connected to a sample input nozzle, an aspiration/dispensing-pump port fluidically connected to a sample aspiration pump, a sample-loop-input port and a sample-loop-output port interconnected by a sample loop, an LC-pump port fluidically connected to an LC pump and a sample-injection-to-trap-bypass-selection port;a trap-bypass-selection valve comprising a plurality of ports and a multi-way switch to switch fluidic connections between ports, including a trap-bypass-selection-to-sample-injection port fluidically connected to the sample-injection-to-trap-bypass-selection port of the sample injection valve, two bypass ports interconnected by a bypass fluidic path, a trap-bypass-selection-to-column-bypass port and two trap-bypass-selection-to-load-elute ports;a column-bypass valve comprising a plurality of ports and a multi-way switch to switch fluidic ...

MODULAR AUTOMATED CHROMATOGRAPHY SYSTEM

Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform may recognize the modules and their locations, coordinate fluid connections between the modules, and provide a variety of control, monitoring, data generating and data processing functions to generate chromatographic data. The software platform may also provide graphical tools for designing chromatographic methods from a library of phases. 1. A system for joining a plurality of fluid manipulation components into a flow scheme for directing fluids to and from a chromatographic separation device and for operating said joined fluid manipulation components according to a selected protocol , said system comprising:a plurality of modules, each said module comprising (a) one of said fluid manipulation components, and (b) a microcontroller that transmits operational signals to said fluid manipulation component in response to commands received from outside said module;a mounting frame comprising a plurality of bays, each of the plurality of bays constructed to receive a single module, and each of the plurality of bays comprising a signal connector that couples to and communicates with the microcontroller of a module mounted in said bay, wherein at least some of the bays are constructed to receive single modules interchangeably with other said modules; anda software platform in communication with the microcontroller of each module, said software being programmed to (1) receive from each of the plurality of modules ...

METHOD FOR PREPARING AT LEAST ONE GENERATOR WITH A HIGH RADIUM-228 CONTENT

A method for preparing one or more generators with a high radium-228 content from an aqueous solution comprising thorium-232 and radium-228. The generator(s) can be used, in particular, for producing thorium-228, from which radium-224, then lead-212 and bismuth-212 can be obtained. The method and the generator(s) that it can be used to prepare are therefore applicable, in particular, in the manufacture of radiopharmaceuticals made from lead-212 or bismuth-212, which can be used in nuclear medicine and, in particular, in targeted alpha radiotherapy for the treatment of cancers. 1. A method for preparing at least one generator comprising radium-228 from an aqueous solution A1 comprising thorium-232 and radium-228 , comprising at least the steps of:a) circulating in a first chromatography column a volume V1 of the aqueous solution A1, the first chromatography column comprising a first stationary phase consisting of a solid material which selectively retains radium with respect to thorium;b) washing at least once the first stationary phase with an aqueous solution A2;c) eluting the radium-228 from the first stationary phase with a volume V3 of an aqueous solution A3 comprising an agent complexing radium-228, the volume V3 being between 0.005% and 1% of the volume V1 of the aqueous solution A1 having circulated in the first chromatography column, whereby an aqueous solution A4 which comprises radium-228 complexes is obtained;d) dissociating the radium-228 complexes present in the aqueous solution A4 by modifying a pH of the aqueous solution A4, whereby an aqueous solution A5 comprising the radium-228 in a decomplexed form is obtained;e) loading a second chromatography column with the aqueous solution A5, the second chromatography column comprising a second stationary phase consisting of a same material as the first stationary phase; andf) washing at least once the second stationary phase with an aqueous solution A6, whereby the at least one generator is obtained.2. The ...

PNEUMATICALLY/HYDRAULICALLY ACTUATED FLUOROPOLYMER-HPLC CHROMATOGRAPHIC SYSTEM FOR USE WITH HARSH REAGENTS

The invention provides a high-performance liquid chromatography system, said system is controlled in temperature by running a fluid in sleeves that surround the different parts of the system. All parts in contact with the fluid are made in fluoropolymer, carbon-filled fluoropolymer, or carbon-fiber fluoropolymer. The system comprises at least one reagent reservoir; at least one mixing chamber, wherein the contents of the at least one reagent reservoir are combined; at least one pump that transfers the contents of the at least one reservoir to the mixing chamber; and at least one modular elution column, wherein the at least one modular elution column contains a temperature control means; a sample injection system connected to an injection loop or 3-way valve to inject the sample solutions in the modular elution columns, at least one manifold or X-Y moving stage to distribute the eluted volumes in different receptacles; at least one return line to automatically reinject selected elution fractions at the top of the column; wherein all moving components of the said system are fluid actuated. 1. A high-performance liquid chromatography system wherein all moving components of that said system are actuated by fluid pressure , said system comprising:a. at least one reagent reservoir, wherein the at least one reservoir contains a temperature control means;b. a mixing chamber, wherein the contents of the at least one reagent reservoir are combined, and wherein the mixing chamber contains a temperature control means;c. a pump that transfers the contents of the at least one reservoir to the mixing chamber;d. a modular elution column, wherein the column contains a temperature control means;e. a means for injecting one or a plurality of sample solutions in the modular elution column;f. a means for distributing eluted volumes to different receptacles; wherein all moving components of the said system are pneumatically actuated andg. a return line with a pump to automatically reload ...

Purification and Separation System and Method

A purification system for separating a molecule of interest from a solution is provided and generally includes a plurality of resin processing sections, wherein each of the resin processing sections is controllably separated from each other via a configurable gate valve, wherein the configurable gate valve is configured to introduce or evacuate a fluid/resin into/out of a proximately located processing section, a plurality of inlets configured to introduce fluid and/or resin into one or more of the plurality of resin processing sections. 1a configurable gate valve;a plurality of inlets; anda plurality of resin processing sections, wherein each of the resin processing sections is controllably separated from each other via the configurable gate valve, wherein the configurable gate valve is configured to introduce or evacuate a fluid/resin into/out of a proximately located processing section, and wherein the plurality of inlets are configured to introduce fluid and/or resin into one or more of the plurality of resin processing sections.. A purification system for separating a molecule of interest from a solution, the system comprising: This application claims the benefit of the filing date of U.S. Provisional Patent Application Ser. No. 61/810,826 filed Apr. 11, 2013 and titled “Purification and Separation System and Method,” the contents of which are incorporated by reference herein in its entirety.This invention relates generally to molecular purification and more particularly to a system for biologically produced molecular purification using a mass transfer technique and article.Molecular purification devices for purifying polypeptides, proteins, polysaccharides, nucleic acids and molecules are known and typically use a traditional chromatography or other established approach. These devices include chromatography columns and include a fixed bed of particles typically consisting of chemically active ligands that are covalently bonded to matrices of polysaccharides, ...

Purification Process

A process is described for removing halogen compounds, particularly chlorine compounds, from a process fluid, comprising the steps of (i) passing a process fluid containing hydrogen halide over a first sorbent to remove hydrogen halide and generate a hydrogen halide depleted process fluid and then, (ii) passing the hydrogen halide depleted process fluid over a second different sorbent to remove organic halide compounds therefrom. A purification system suitable for removing hydrogen halide and organic halide compounds from process fluids is also described. 1. A process for removing a halogen compound from a process fluid , comprising the steps of:(i) passing a process fluid containing hydrogen halide over a first sorbent comprising an alkalized alumina to remove hydrogen halide and generate a hydrogen halide depleted process fluid; and(ii) passing the hydrogen halide depleted process fluid over a second sorbent comprising zeolite 13X to remove an organic halide compound.2. The process of claim 1 , wherein the process fluid is a hydrogen gas stream comprising 50% vol or greater of hydrogen.3. The process of claim 1 , wherein the process fluid is a liquid stream comprising a hydrocarbon or a gas stream comprising a hydrocarbon.4. The process of claim 1 , wherein the process fluid is a liquid stream comprising a hydrocarbon.5. The process of claim 1 , wherein the halogen compound is a bromine compound or a chlorine compound.6. The process of claim 5 , wherein the halogen compound is a chlorine compound.7. The process of claim 1 , wherein the hydrogen halide content of the process fluid fed to the first sorbent is in the range of from 0.1 to 20 ppm.8. The process of claim 1 , wherein the first sorbent comprises acidic sites that form one or more organic halide compounds.9. The process of claim 1 , wherein the process fluid from step (ii) is passed over a third sorbent to remove residual or formed hydrogen halide.10. The process of claim 9 , wherein the third sorbent is ...

ISOTOPE PREPARATION METHOD

The present invention comprises a method for the generation of Th of pharmaceutically tolerable purity comprising i) preparing a generator mixture comprising Ac, Th and Ra; ii) loading said generator mixture onto a strong base anion exchange resin; iii) eluting a mixture of said Ra and Ac from said strong base anion exchange resin using a first mineral acid in an aqueous solution; iv) eluting Th from said strong base anion exchange resin using a second mineral acid in an aqueous solution whereby to generate a first Th solution containing contaminant Ra and Ac; v) loading the first Th solution onto a strong acid cation exchange resin; vi) eluting at least a part of the contaminant Ra and Ac from said strong acid cation exchange resin using a third mineral acid in aqueous solution; and vii) eluting the Th from said strong acid cation exchange resin using a first aqueous buffer solution to provide a second Th solution. Purified thorium-227 of pharmaceutical purity and a pharmaceutical composition comprising the same are also provided. 1) A method for the generation of Th of pharmaceutically tolerable purity comprising the steps of:{'sup': 227', '227', '223, 'i) preparing a generator mixture comprising Ac, Th and Ra;'}ii) loading said generator mixture onto a strong base anion exchange resin;{'sup': 223', '227, 'iii) eluting a mixture of said Ra and Ac from said strong base anion exchange resin using a first mineral acid in an aqueous solution;'}{'sup': 227', '227', '223', '227, 'iv) eluting Th from said strong base anion exchange resin using a second mineral acid in an aqueous solution whereby to generate a first Th solution containing contaminant Ra and Ac;'}{'sup': '227', 'v) loading the first Th solution onto a strong acid cation exchange resin;'}{'sup': 223', '227, 'vi) eluting at least a part of the contaminant Ra and Ac from said strong acid cation exchange resin using a third mineral acid in aqueous solution; and'}{'sup': 227', '227, 'vii) eluting the Th from ...

Xylene separation process

A process is described for separating paraxylene from a multicomponent fluid mixture of C8 aromatics. A mixture of C8 aromatics is fed to a simulated moving-bed adsorptive apparatus. The location of the feed to the apparatus is moved at set intervals. The rate of flow of feed to the apparatus is varied during each interval to enhance the separation of paraxylene from the multicomponent mixture.

MULTIPLE COLUMN CHROMATOGRAPHIC SYSTEM AND METHODS OF USE

A chromatographic apparatus includes two chromatographic columns configured to perform chromatography. The apparatus further includes a primary valve disposed upstream of the first chromatographic column and the second chromatographic column. The primary valve selectively switches between a first position in which the pump is in fluid communication with a first path that includes the first chromatographic column and a second position in which the pump is in fluid communication with a second path that includes the second chromatographic column. The apparatus also includes a first and a second sample injection port associated with said first and second columns, respectively, and at least one detector. The apparatus is configured such that flow from the column in the selected path goes to said at least one detector without passing through the other column. 1. A chromatographic apparatus comprising:a first chromatographic column configured to perform a first type of chromatography;a second chromatographic column configured to perform a second type of chromatography;a pump configured to be in selective fluid communication with either the first chromatographic column or the second chromatographic column, the pump being upstream of the columns; a first position in which the pump is in fluid communication with a first path that includes the first chromatographic column; and', 'a second position in which the pump is in fluid communication with a second path that includes the second chromatographic column;, 'a primary valve disposed upstream of the first chromatographic column and the second chromatographic column and downstream of the pump, the primary valve configured to selectively switch between'}a first and a second sample injection port associated with said first and second columns, respectively; andat least one detector downstream of said columns;wherein the apparatus is configured such that flow from the column in the selected path goes to said at least one detector ...

PROCESS FOR PURIFICATION AND SEPARATION OF CANNABINOIDS, FROM DRIED HEMP AND CANNABIS LEAVES

Disclosed is a method for purification and separation of cannabinoids, specifically cannabidiol and tetrahydrocannabinol, from the dried hemp and leaves using a combination of a sequence of purification steps including: filtration, decolorization, activation or decarboxylation, dewaxing, a continuous simulated moving bed process, polishing, and crystallization to separate cannabinoids from tetrahydrocannabinol and to provide phytocannabinoid rich oil and cannabidiol isolate. The cannabinoid products can be used in various pharmaceutical and nutraceutical applications. 1cannabis. A process for the purification of cannabidiol (CBD) in a crude extract stream to provide at least one high purity cannabidiol product selected from the group consisting of a high purity cannabinoid oil stream , a phytocannabinoid rich oil , a solid CBD aggregate and mixtures thereof being essentially free of tetrahydrocannabinol , said process comprising:{'i': 'cannabis', 'a) passing the crude extract stream comprising debris and small particles, cannabidiol, tetrahydrocannabinol, cannabidiolic acid, tetrahydrocannabinolic acid, other cannabinols, chlorophylls, color bodies, sugars and carbohydrates, lipids, plant waxes, impurities, and ethanol to a first filtration zone comprising a series of successive filters of decreasing pore size, starting at a pore size of 100 microns and reducing to about 10 microns in 3 or more stages to remove debris and small particles in a progressive filtration step to provide a filtered crude cannabinoid stream;'}b) passing the filtered crude cannabinoid stream comprising cannabidiol, tetrahydrocannabinol, cannabidiolic acid, tetrahydrocannabinolic acid, other cannabinols, chlorophylls, color bodies, sugars and carbohydrates, lipids, plant waxes, impurities, and ethanol to a decolorization zone comprising a 10 μm filter and a decolorization chromatographic column containing a modified activated carbon adsorbent which was heat treated to provide a highly ...

PROCESS FOR PURIFICATION AND SEPARATION OF CANNABINOIDS, FROM DRIED HEMP AND CANNABIS LEAVES

A method for purification and separation of cannabinoids, such as cannabidiol and tetrahydrocannabinol, e.g., from the dried hemp and leaves can use a continuous simulated moving bed process and a combination of one or more of a sequence of purification steps including: filtration, decolorization, activation or decarboxylation, dewaxing, polishing, and crystallization to separate a cannabinoid from the plant and to provide various cannabinoid products. The cannabinoid products can be used in various pharmaceutical and nutraceutical applications. 1cannabiscannabis. A method of separating a cannabinoid from a plant , the plant including the cannabinoid and at least one impurity , the method comprising:{'i': cannabis', 'cannabis, 'combining the plant and a solvent to form a crude extract stream;'}{'i': cannabis', 'cannabis, 'processing the crude extract stream into a simulated moving bed (SMB) feedstock stream by removing at least a portion of at least one impurity in the crude extract stream; and'}passing the SMB feedstock stream through a SMB zone to provide a primary raffinate stream having a higher purity of the cannabinoid than in the SMB feedstock stream as measured by weight percentage of the solid content and a SMB extract stream having a lower purity of the cannabinoid than in the SMB feedstock stream as measured by weight percentage of the solid content.2cannabisCannabis sativa, Cannabis indica, Cannabis rudralis. The method of claim 1 , wherein the plant is selected from claim 1 , and mixtures thereof.3cannabiscannabis. The method of claim 1 , wherein the plant comprises dried hemp claim 1 , leaves claim 1 , or a mixture thereof.4. The method of claim 1 , wherein the solvent comprises ethanol.5cannabiscannabiscannabiscannabiscannabiscannabiscannabiscannabiscannabis. The method of claim 1 , wherein said at least one impurity comprises at least one of color bodies claim 1 , acidic components claim 1 , lipids claim 1 , and plant waxes claim 1 , and wherein ...

PROCESS FOR SEPARATING A CONSTITUENT/CANNABINOID USING A CHROMATOGRAPHIC RESIN

A method for purification and separation of cannabinoids, such as cannabidiol and tetrahydrocannabinol, e.g., from dried hemp and leaves can use a continuous simulated moving bed process, a batch column chromatography method, or a single column, and a combination of one or more of a sequence of purification steps including: filtration, decolorization, activation or decarboxylation, dewaxing, polishing, and crystallization to separate a cannabinoid from the plant and to provide various cannabinoid products. The cannabinoid products can be used in various pharmaceutical and nutraceutical applications. 1cannabiscannabis. A method of separating a cannabinoid of a plant , the plant including the cannabinoid and at least one impurity , the method comprising:{'i': 'cannabis', 'preparing a feedstock stream, the feedstock stream including the plant and a solvent;'} a first resin, the first resin comprising a modified activated carbon adsorbent having an average particle size range of from about 45 to about 1700 microns,', 'a second resin, the second resin comprising a modified hydrophobic adsorbent having an average bulk density of from about 0.4 g/mL to about 0.6 g/mL, the modified hydrophobic adsorbent comprising at least one of a styrene-divinylbenzene (DVB) resin or a poly(methyl methacrylate) (PMMA) resin,', 'a third resin, the third resin comprising a hydrophobic resin having an average bulk density of from about 0.75 g/mL to about 0.85 g/mL,', 'a fourth resin, the fourth resin comprising a hydrophobic polystyrene-divinylbenzene adsorbent having a water content of from about 55% to about 65%, and', 'any mixture thereof., 'passing the feedstock stream through a chromatographic resin to provide an eluate stream, the eluate stream having a higher purity of the cannabinoid than in the feedstock stream as measured by weight percentage of the solid content, wherein the chromatographic resin comprises at least one of2. The method of claim 1 , wherein the cannabinoid is CBD ...

A PROCESS FOR PURIFICATION OF POLYETHER BLOCK COPOLYMERS

Process for providing purified polyether block copolymers comprising polyoxyethylene (PEO) and polyoxypropylene (PPO) moieties wherein the purified product is obtained by an ultrafiltration step of a solution of the polyether block copolymers and wherein the block copolymers are depleted in lower molecular impurities. 1. A process for providing purified polyether block copolymers comprising polyoxyethylene (PEO) and polyoxypropylene (PPO) moieties wherein the purified polyether block copolymer is obtained by an ultrafiltration step of a solution of the polyether block copolymer.2. The process according to claim 1 , wherein the polyether block copolymers comprising polyoxyethylene and polyoxypropylene moieties are selected from the group consisting of polyethylene oxide-block-polypropylene oxide copolymers and polyethylene oxide-polypropylene oxide random copolymers.3. The process according to claim 1 , wherein the polyether block copolymers comprising polyoxyethylene and polyoxypropylene moieties are triblock (PEO-PPO-PEO)-copolymers4. The process according to wherein the triblock (PEO-PPO-PEO)-copolymers are poloxamer 188 or poloxamer 407.5. The process according to claim 1 , wherein the solution of the polyether block copolymers treated by ultrafiltration is an aqueous solution or an organic solution or an aqueous-organic solvent mixture.6. The process according to claim 1 , wherein the solution of the polyether block copolymers treated by ultrafiltration is an aqueous solution.7. The process according to claim 1 , wherein a concentration of the polymer in the solution is 2-20% b.w.8. The process according to claim 1 , wherein a concentration of the polymer in the solution is 5-15% b.w.9. The process according to claim 1 , wherein the ultrafiltration is carried out using a membrane separating layer which is a ceramic or polymer or carbon material.10. The process according to claim 1 , wherein the ultrafiltration is carried out using a membrane separating layer ...

SIMULATED MOVING-BED TYPE CHROMATOGRAPHIC SEPARATION METHOD AND SIMULATED MOVING-BED TYPE CHROMATOGRAPHIC SEPARATION SYSTEM

A simulated moving-bed type chromatographic separation method separating a weakly adsorptive component, a strongly adsorptive component, and an intermediately adsorptive component, with eluents by using a circulation system in which a plurality of unit packed columns packed with an adsorbent are connected in series and in an endless form via pipes in which a feed solution supply port F, two or more eluent supply ports D corresponding to the eluents, an extraction port A of a fraction containing the weakly adsorptive component, an extraction port B of a fraction containing the intermediately adsorptive component, and an extraction port C of a fraction containing the strongly adsorptive component are provided in the pipes of the circulation system, and positions of the ports F, A, B, and C are set to have a specified relationship. 1. A simulated moving-bed type chromatographic separation method comprising separating a weakly adsorptive component , a strongly adsorptive component , and an intermediately adsorptive component that has intermediate adsorptive property in relation to both components , with respect to an adsorbent , with two or more kinds of eluents by using a circulation system in which a plurality of unit packed columns packed with the adsorbent are connected in series and in an endless form via pipes , the weakly adsorptive component , the strongly adsorptive component , and the intermediately adsorptive component being contained in a feed solution , wherein a feed solution supply port F , two or more eluent supply ports D corresponding to the two or more kinds of eluents , an extraction port A of a weakly adsorptive fraction containing the weakly adsorptive component , an extraction port B of an intermediately adsorptive fraction containing the intermediately adsorptive component , and an extraction port C of a strongly adsorptive fraction containing the strongly adsorptive component are provided in the pipes of the circulation system , and positions of ...

Quantum Fluid Operation: Technology for Effective Mixing, Reacting, and Separating Fluids

A continuous chemical process is modified to allow parts thereto to be processed one quantum of matter at a time. This offers precision and efficiency beyond what is possible with the continuous mode. This Quantum Fluid Operation (QFO) is applied to basic unit operations: mixing, reacting, separating. 1. A system called “Quantum Fluid Operation” (QFO) for secluding a quantum of fluid in a continuous industrial flow , and treating this quantum as batch operation without affecting the control flow before and after the QFO; the QFO comprising:(i) input fluid capacity tanks, (A tanks),(ii) output fluid capacity tanks, (C tanks),(iii) a quantum fluid container, (B),(iv) operational implements,{'sub': 1', '2', 'f', 'a', 'c', 'a', 'in, 'the total flow of all the fluids a, a, . . . athrough the f A tanks is at a constant flow rate q, and the constant flow rate to the output fluid capacity tank is q=q; a quantum of fluid of measure Q is taken out of the f A tanks during time interval t, and is accumulated in the quantum fluid container, (B), which is big enough to contain the quantum fluid;'}{'sub': 'q', 'next the operational implements operate (treat) on the quantum Q contained in B for a period of time t;'}{'sub': 'out', 'claim-text': {'br': None, 'i': Q=q', 't', '+t', '+t', 'q', 't', '+t', '+t, 'sub': a', 'in', 'q', 'out', 'c', 'in', 'q', 'out, '*()=*()'}, 'next the quantum Q is pumped out of the container B to output fluid capacity tanks, (C), over a period of time t, the identified flow rates and timings comply with the following equation{'sub': 'q', 'and where the operational implements operating on the quantum of fluid Q, change the state of the quantum to a desired state, S=S(Q), and where the quantum treating time, t, is extended as needed to insure that the quantum of fluid, Q, leaves the quantum fluid container, (B) at the desired state, S(Q).'}2. The system in where the desired state of Q claim 1 , S(Q) is expressed as the desired temperature of Q claim 1 , (T) ...

Simulated Moving Bed Chromatographic Separation Process

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product, from a feed mixture, which process comprises introducing the feed mixture to a simulated or actual moving bed chromatography apparatus having a plurality of linked chromatography columns containing, as eluent, an aqueous alcohol, wherein the apparatus has a plurality of zones comprising at least a first zone and a second zone, each zone having an extract stream and a raffinate stream from which liquid can be collected from said plurality of linked chromatography columns, and wherein (a) a raffinate stream containing the PUFA product together with more polar components is collected from a column in the first zone and introduced to a nonadjacent column in the second zone, and/or (b) an extract stream containing the PUFA product together with less polar components is collected from a column in the second zone and introduced to a nonadjacent column in the first zone, said PUFA product being separated from different components of the feed mixture in each zone. 1. A composition comprising a PUFA product , wherein the PUFA product is EPA present in an amount greater than 93 wt % , wherein the total content of ω-6 polyunsaturated fatty acids is up to 0.40 wt % , and wherein the total content of eicosatetraenoic acid is up to 1 wt %.2. The composition according to claim 1 , wherein the total content of eicosatetraenoic acid is 0.05 wt % or greater.3. The composition according to claim 1 , wherein the composition (a) comprises greater than 96.5 wt % EPA claim 1 , up to 1 wt % DHA claim 1 , up to 1 wt % α-linolenic acid claim 1 , up to 1 wt % stearidonic acid claim 1 , up to 1 wt % eicosatetraenoic acid claim 1 , up to 1 wt % docosapentaenoic acid claim 1 , and up to 0.25 wt % arachidonic acid; or(b) comprises greater than 96.5 wt % EPA, up to 0.2 wt % DHA, up to 0.3 wt % α-linolenic acid, up to 0.4 wt % stearidonic acid, up to 0.5 wt % ...

Planetary Countercurrent Chromatography Centrifuge and Mixer-Settler Rotor

A mixer-settler countercurrent chromatography centrifuge and rotor of increased capacity is described. 2. The centrifuge of wherein said shafts comprise a sealed bearing.3. The centrifuge of wherein said shafts comprise a terminal flared portion.4. The centrifuge of claim 1 , comprising at least six disks.5. The centrifuge of claim 1 , comprising at least eight discs.6. The centrifuge of wherein said discs comprise a raised lip about an outer edge of said disk.7. The centrifuge of wherein said discs comprise a raised lip about an inner edge of said disk.8. The centrifuge of wherein said gaskets seat within said raised lip.9. The centrifuge of wherein said gaskets seat within said raised lip.10. The centrifuge of wherein said rotor comprises ribbed endplates.11. The centrifuge of wherein said rotor comprises a tubing protector.12. The centrifuge of wherein said tubing protector comprises a sleeve.13. The centrifuge of wherein said sleeve is corrugated.14. The centrifuge of wherein said tubing protector comprises a foam claim 11 , a sponge or a hydrogel. Portions of the research described herein were supported in part by NIH grant no. R43AT008296-01 to CC Biotech, Rockville, Md.Carbon nanotubes (CNT), carbon-carbon extended polymers with fused sp2 orbitals, have aromatic properties [1,2]. Sheets are known as graphenes and can form tubes, nanotubes. Tubular forms are of various diameter from about 1 rim to a few hundred urn and have lengths of about 500 nm to several thousands of nanometers. Nanotubes are single-walled or multi-walled, having anisotropic structures. Nanotubes exhibit conductive or semiconductor properties, and are chiral. The hexagonal array of the atoms is in a left-handed or a right-handed spiral pattern. Dimensions of patterns of a hexagonal honeycomb lattice are described by vectors, m and n, where nanotubes of certain values of m and n being semiconducting. A, ‘zig-zag,’ pattern where m=0 and an, ‘arm chair,’ pattern where n=m (metallic) are non- ...

Multi-Vessel Filtration System for Hazardous or Radioactive Waste Water

Surface or submersible sluiceable systems are disclosed for use in removing hazardous contaminants or radioactive isotopes from a fluid stream, such as a fluid stream from the primary coolant loop or secondary loop of a nuclear reactor system, or a fluid stream from a spent-fuel pool or pond or hazardous or radioactive contaminants in ground water. Generally, this surface or submersible sluiceable system is adapted to be utilized in a surface skid or submersed in the fluid stream, and additionally the vessels are adapted to be sluiced and reused after use, resulting in a potentially stabilized, non-leaching final waste product with a substantially reduced volume for storage or disposal. The system can be utilized with standard ion exchange beads or preferably with inorganic granular media. 1. A surface or submersible sluiceable lead-lag system to remove selected hazardous contaminants or radioactive isotopes from fluid waste materials , the system comprising: a vessel body having an interior volume,', 'a fill head having a plurality of ports giving access to the interior of said vessel body, including a fluid waste material input port, a treated fluid waste material output port, a sluice-in port to facilitate delivery of media to the interior of said vessel body, a sluice-out port to facilitate removal of spent media from the interior of said vessel body, and a sluice water input port,', 'internal media containment screens within the vessel body,', 'an internal waste fluid line to deliver fluid waste materials from said fluid waste material input port to a location within the interior of the vessel body, said location being placed such that fluid waste materials exiting the internal waste fluid line pass through said media before exiting the interior of the vessel body through said treated fluid waste material output port,', 'a sluice-in tube to deliver media into the vessel during filling, and', 'a sluice-out tube to remove media from the vessel, said sluice-out ...

ANTIBODIES COMPRISING SEQUENCES FROM CAMELIDAE TO HIGHLY CONSERVED TARGETS

The present invention is concerned with antigen binding polypeptides, and in particular conventional antibodies derived from camelid species, that specifically bind to target antigens that are either self-antigens or highly conserved antigens. The present invention also relates to anti-idiotype antigen binding peptides which bind to a target antigen comprising the variable region of an antibody obtained from a species within the family Camelidae. 117-. (canceled)18. A method for preparing a recombinant antigen binding polypeptide that specifically binds to a target antigen , said antigen binding polypeptide comprising a VH domain and a VL domain , wherein at least one hypervariable loop or complementarity determining region (CDR) in the VH domain or the VL domain is obtained from a species in the family Camelidae , wherein the target antigen is a polypeptide antigen that exhibits at least 90% amino acid sequence identity with a protein from said species in the family Camelidae over a sequence comparison window , said method comprising the step of:(a) immunizing a species in the family Camelidae with the target antigen, thereby raising a conventional antibody to said target antigen.19. (canceled)20. (canceled)21. The method of claim 18 , wherein the species in the family Camelidae is selected from the group consisting of camel claim 18 , llama claim 18 , dromedary claim 18 , vicuna claim 18 , guanaco claim 18 , and alpaca.2233-. (canceled)34. The method of claim 18 , wherein the sequence comparison window includes at least the epitope for the antigen binding polypeptide.35. The method of claim 18 , wherein the sequence comparison window comprises at least one domain of the target antigen claim 18 , including the epitope for the antigen binding polypeptide.36. The method of claim 18 , wherein the sequence comparison window includes the full length of the target antigen.37. The method of claim 18 , further comprising the steps of:(b) isolating Camelidae nucleic acid ...

METHOD FOR TREATING LIGNOCELLULOSIC MATERIALS

A method of generating a refined sugar stream that comprises xylose from a biomass hydrolysis solution, including contacting a biomass hydrolysis solution that includes a population of mixed sugars comprising xylose, an acid, and impurities, with a thermally-phase separable solvent such as a glycol solvent to form an extraction mixture; and separating from said extraction mixture a first stream including the thermally-phase separable solvent, acid, and impurities and a second, refined sugar stream that comprises xylose. The thermally-phase separable solvent is an ethylene glycol or a propylene glycol ether, such as 2-butoxyethanol or 1-propoxy-propanol or any combination thereof. 1. A method of generating a refined a sugar stream that comprises xylose from a biomass hydrolysis solution , comprising:(i) contacting a biomass hydrolysis solution that comprises a population of mixed sugars comprising xylose, an acid, and impurities, with a thermally-phase separable solvent to form an extraction mixture; and(ii) separating from said extraction mixture a first stream comprising the thermally-phase separable solvent, acid, and impurities and a second, refined sugar stream that comprises xylose.2. The method of claim 1 , further comprising claim 1 , contacting a stream from said biomass hydrolysis solution claim 1 , which comprises said population of mixed sugars comprising xylose with a strong acid cation exchange resin prior to step (i).3. The method of claim 2 , further comprising claim 2 , contacting a stream from said biomass hydrolysis solution claim 2 , which comprises said population of mixed sugars comprising xylose with a weak base anion exchange resin after said stream is contacted with said strong acid cation exchange resin and prior to step (i).4. The method of claim 1 , further comprising heating said extraction mixture to a temperature of 30-100° C.5. The method of claim 1 , further comprising separating said second claim 1 , refined sugar stream that ...

MULTI-STEP SEPARATION PROCESS

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which comprises: (a) purifying the feed mixture in a first chromatographic separation step using an eluent a mixture of water and a first organic solvent, to obtain an intermediate product; and (b) purifying the intermediate product in a second chromatographic separation step using as eluent a mixture of water and a second organic solvent, to obtain the PUFA product, wherein the second organic solvent is different from the first organic solvent and has a polarity index which differs from the polarity index of the first organic solvent by between 0.1 and 2.0, wherein the PUFA product is other than alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), linoleic acid, an ALA mono- di- or triglyceride, a GLA mono- di- or triglyceride, a linoleic acid mono- di- or triglyceride, an ALA C-Calkyl ester, a GLA C-Calkyl ester or a linoleic acid C-Calkyl ester or a mixture thereof. 2. The process according to claim 1 , wherein the first chromatographic separation step consists of two chromatographic separations claim 1 , wherein each separation uses as eluent a mixture of water and the first organic solvent.3. The process according to claim 1 , wherein the second chromatographic separation step consists of two chromatographic separations claim 1 , wherein each separation uses as eluent a mixture of water and the second organic solvent.4. The process according to claim 1 , wherein the first and second organic solvents are chosen from alcohols claim 1 , ethers claim 1 , esters claim 1 , ketones and nitriles.5. The process according to claim 4 , wherein the ketone is acetone claim 4 , methylethylketone or methyl isobutyl ketone (MIBK) claim 4 , preferably acetone.6. The process according to claim 1 , wherein one of the first and second organic solvents is methanol.7. The process according to claim 1 , wherein the second organic ...

Method for producing lead-212 from an aqueous solution comprising thorium-228 and daughters thereof

A method for producing lead-212 of very high radiological purity from an aqueous solution comprising thorium-228 and daughters thereof. Manufacture of radiopharmaceuticals based on lead-212, which are useful in nuclear medicine and, in particular, in targeted alpha radiation therapy for the treatment of cancers.

Continuous processing methods for biological products

The present invention is directed to the development of continuous processing technology for the purification of biopharmaceuticals and biological products, such as monoclonal antibodies, protein therapeutics, and vaccines. Methods for continuous processing of a biological product in a feed stream toward formulation of a purified bulk product are described.

ION EXCHANGE EXOSKELETON AND FILTER ASSEMBLY

An ion exchange filter for a coolant may include a porous ion exchange filter exoskeleton and ion exchange resin beads. The exoskeleton may be adapted for receiving a coolant flow and may define a first set of channels. The ion exchange resin beads may be located within the first set of channels. 1. An ion exchange exoskeleton for an ion exchange filter for a coolant comprising:a porous sheet comprising a plurality of pleats;a non-porous sheet overlaid and contacting the porous sheet, wherein the non-porous sheet is not pleated;wherein the non-porous sheet overlaid onto the porous sheet forms a multi-layer exoskeleton sheet;wherein the ion exchange exoskeleton is a spiral wrapping of the exoskeleton sheet about a central axis, the exoskeleton sheet encircling the central axis multiple times forming a plurality of spirally wrapped radially stacked layers of the exoskeleton sheet radially overlaid directly onto each other around the central axis, wherein axial herein is a direction parallel to the central axis; an inlet face for receiving coolant flow;', 'an outlet face for discharging coolant;', 'wherein the inlet and outlet faces are arranged on opposite axial end faces of the ion exchange exoskeleton;, 'wherein the ion exchange skeleton includeswherein the porous sheet has a plurality of axial pleats, each pleat extending axially from the inlet face to the outlet face of the of the ion exchange exoskeleton, the pleats forming a first set of flow channels extending axially from the inlet face to the outlet, each flow channel opening at the inlet face to receive flow into the ion exchange exoskeleton and opening at the outlet face to discharge flow from the ion exchange exoskeleton;a first set of flow channels formed by the plurality of axial pleats, the first set of flow channels extending axially completely through an interior of the ion exchange exoskeleton, each having a channel cross section that is fully open to an exterior at the inlet face and the outlet face ...

METHODS AND SYSTEMS FOR DETERMINING MULTI-COLUMN CHROMATOGRAPHY PROCESS CONFIGURATION

The disclosure generally relates to methods and systems for determining multi-column chromatography process configuration for capturing antibodies. Conventional approaches for design of MCC configuration are limited to rule based, either driven by UV spectroscopic measurements or by performing number of experiments, which involves a lot of material costs and time utilization. The present disclosure solves the technical problem of identifying the operational conditions that optimized the MCC process and the MCC configuration. A multi-objective optimization function defined with one or more decision variables associated with the operating conditions is considered to determine the optimal MCC configuration, while satisfying purification goals. The one or more key performance measures of the MCC process comprises a productivity, a capacity utilization, a product yield, and a product purity. The significant amount of time and the material cost invested for designing the optimum MCC configuration is decreased by the present disclosure. 1. A processor-implemented method for determining an optimum multi-column chromatography (MCC) configuration of an MCC process , the method comprising the steps of:receiving, via one or more hardware processors, one or more of: feed characteristics, targets for some of one or more key performance measures of the MCC process, one or more MCC configurations of the MCC process, one or more column characteristics of each column of one or more columns present in each MCC configuration, and media characteristics of one or more chromatography media for use in each column of one or more columns;selecting, via the one or more hardware processors, a chromatography column (CC) process model set from a plurality of CC process model sets, based on the media characteristics of the one or more chromatography media for use in each column of the one or more columns and one or more components present in the feed, wherein each CC process model set comprises ( ...

Chromatography columns and processes

A separation column for expanded bed adsorption comprises a column tube ( 2 ), a base ( 15 ) carrying an inlet rotor structure ( 6 ) for pumping in process liquid, and a top cap ( 3 ). The top cap ( 3 ) is conical in form, and has a peripheral flange 31 by which it is rigidly fixed to the top edge flange ( 22 ) of the column tube ( 2 ). The angle of the convergent interior surface ( 35 ) of the conical top cap ( 3 ) may be between 10 and 25°. A vortex-inhibitor component ( 8 ) projects down below the outlet structure ( 4 ) at the top of the cap, projecting into the operating space ( 15 ) of the column to inhibit rotation of liquid in the column interior. An expanded bed adsorption process is done with upward flow of liquid in the column through a bed of media particles.

Regulated Method For Separating A Mixture

The invention relates to a method for separating a mixture in a system comprising a plurality of chromatography columns, the method successively comprising, in a cyclical manner, in a given part of the system: a step of collecting a raffinate; a step of injecting the mixture to be separated; a step of collecting an extract; and a step of injecting a mobile phase; the method further comprising: determining, in a node of the system, the history of a variable representing the concentration of at least one species contained in the mixture to be separated; detecting, within said history, a characteristic point between the beginning of a step of collecting the extract and the end of the following step of collecting the raffinate; comparing the position of the characteristic point in relation to a target position; and adjusting the carrier volume of the characteristic point, modifying the position of the characteristic point in order to bring the position of the characteristic point closer to the target position; the volume of the mobile phase injected per cycle being maintained higher than, or equal to, a minimum threshold and/or lower than, or equal to, a maximum threshold. The invention also relates to a computer program for carrying out the steps of such a method, to a storage medium on which such a program is recorded, and to a system comprising a processor coupled to a memory on which such a program is recorded. 1. Method of separating a mixture in a system comprising a plurality of chromatography columns , the method successively comprising , in a cyclic manner , in a given part of the system:a step of collecting a raffinate, a step of injecting the mixture to be separated, astep of collecting an extract, and a step of injecting a mobile phase;wherein the method further comprises:the determination, in a node of the system, of the history of a variable that is representative of the concentration of one or more species contained in the mixture to be separated;the ...

Systems and Methods for Separating Radium from Lead, Bismuth, and Thorium

Systems for separating Ra from a mixture comprising at least Ra, Pb, Bi, and Th are provided. The systems can include: a first vessel housing a first media and Th or Bi; a second vessel in fluid communication with the first vessel, the second vessel housing a second media and Pb; and a third vessel in fluid communication with the second vessel, the third vessel housing a third media and Ra, wherein at least one of the first, second, or third medias are different from the other media. 1. A system for separating Ra from a mixture comprising at least Ra , Pb , Bi , and Th , the system comprising:a first vessel housing a first media and either Pb or Bi and/or Th; anda second vessel in fluid communication with the first vessel, the second vessel housing a second media and Ra, wherein the first media is different from the second media.2. The system of wherein the first media is associated with Bi and/or Th and comprises a quaternary amine on a polystyrene divinylbenzene copolymer.3. The system of wherein the second media is associated with Ra and comprises a silica support.4. The system of wherein the first media is associated with Pb and comprises 18-crown-6 and 1-octanol on Amberchrom CG-71 polymer support.5. The system of wherein the second media is associated with Ra comprises a on silica support.6. The system of wherein the first media size is less than 100 μm.7. The system of wherein the second media size is greater than 100 μm.8. A system for separating Ra from a mixture comprising at least Ra claim 1 , Pb claim 1 , Bi claim 1 , and Th claim 1 , the system comprising:a first vessel housing a first media and Th and/or Bi; anda second vessel in fluid communication with the first vessel, the second vessel housing a first media and Pb, wherein the first media is different from the second media.9. The system of wherein the first vessel is in fluid communication with raw material supply.10. The system of wherein the first vessel is in fluid communication with a wash ...

METHOD FOR SEPARATING STEVIOL GLYCOSIDE, METHOD FOR PRODUCING REBAUDIOSIDE A, AND DEVICE FOR SEPARATING STEVIOL GLYCOSIDE

A method for separating steviol glycoside, including: a separating step of performing a continuous liquid chromatography for continuously separating at least one type of steviol glycoside by allowing a liquid to be separated containing plural types of steviol glycosides to pass through a separating agent in which polyethylene imine is immobilized to a carrier. 1. A method for separating steviol glycoside , comprising:a separating step of performing a continuous liquid chromatography for continuously separating at least one type of steviol glycoside by allowing a liquid to be separated containing a plurality of types of steviol glycosides to pass through a separating agent in which polyethylene imine is immobilized to a carrier.2. The method for separating steviol glycoside according to claim 1 ,wherein the carrier is a macromolecular carrier.3. The method for separating steviol glycoside according to claim 1 ,wherein the liquid to be separated contains rebaudioside A as the steviol glycoside, andthe rebaudioside A is separated from the liquid to be separated.4. The method for separating steviol glycoside according to claim 3 ,wherein the liquid to be separated further contains stevioside as the steviol glycoside, andeach of the rebaudioside A and the stevioside is separated from the liquid to be separated.5. The method for separating steviol glycoside according to claim 3 ,wherein the liquid to be separated contains lower alcohol having carbon atoms of lower than or equal to 4 as a solvent.6. The method for separating steviol glycoside according to claim 1 ,wherein the continuous liquid chromatography is performed by using a simulated moving bed type device.7. A method for producing rebaudioside A claim 1 , comprising:a separating step of performing a continuous liquid chromatography for continuously separating rebaudioside A by allowing a liquid to be separated containing a plurality of types of steviol glycosides containing the rebaudioside A to pass through a ...

Phosphonic acid catalyst in dehydrative cyclization of 5 and 6 carbon polyols with improved color and product accountability

A process for preparing cyclic dehydration products from sugar alcohols is described. The process involve using a mixed-acid catalyst reaction mixture containing a reducing acid, having a pKa of about 1.0-1.5, and at least a strong Brønsted acid or a Lewis acid, having a pKa≦0, or both acids in a solution to dehydrate and ring close said sugar alcohol. Synergistically, the mixed-acid catalysis can produce greater amounts of the desired product at similar levels of compositional accountability than either of the component acid catalysts acting alone.

SYSTEMS, APPARATUS AND METHODS FOR SEPARATING ACTINIUM, RADIUM, AND THORIUM

A method of separating actinium and/or radium from proton-irradiated thorium metal. The thorium metal is irradiated to produce isotopes including thorium, actinium and/or radium. The resultant product is dissolved in solution and a selective precipitant is used to precipitate a bulk portion of the thorium. The precipitated thorium can be recovered. Chromatography is carried out on the remaining solution to remove residual thorium and to separate the actinium from the radium. 1. A method of separating thorium from actinium and/or radium , the method comprising:placing the thorium and the actinium and/or radium in a weak acid solution;adding a selective precipitant to the weak acid solution and precipitating a bulk portion of the dissolved thorium under precipitation conditions while leaving the actinium and/or radium in the solution; andfiltering to separate the precipitated bulk portion of the thorium from the actinium and/or radium in the solution.2. A method of separating actinium and/or radium from thorium , the method comprising the steps of:placing the thorium and the actinium and/or radium in a weak acid to yield a first solution;adding a selective precipitant and precipitating a bulk portion of the thorium under precipitation conditions while retaining the actinium and/or radium and a residual portion of the thorium in a second solution;filtering to separate the precipitated bulk portion of the thorium from the second solution; andconducting chromatographic purification of the second solution to separate the actinium and/or radium from the residual thorium.3. A method as defined in claim 2 , wherein the thorium and the actinium and/or radium are produced by irradiating thorium metal claim 2 , and wherein the irradiated thorium metal is dissolved in the weak acid to yield the first solution.4. A method of producing thorium radioisotopes claim 2 , the method comprising:irradiating thorium metal to produce thorium radioisotopes;dissolving the irradiated thorium ...

CONTINUOUS COUNTERCURRENT SPIRAL CHROMATOGRAPHY

A system, module and method for continuous countercurrent spiral chromatography are disclosed. The module includes an input port for receiving an input solution, a first mixer for mixing the input solution with a recycled solution to produce a first mixed output, a stage I separator for concentrating the first mixed output to produce a stage I solid fraction, a second mixer for mixing the stage I solid fraction from the stage I separator and an optional buffer solution to produce a second mixed output, and a stage II separator for concentrating the second mixed output to produce a stage II solid fraction which exits the module. At least one separator is a spiral separator. The system includes a plurality of modules, and at least one of the plurality of modules includes a spiral separator. The method includes purifying an unpurified solution with the plurality of modules. 1. A continuous countercurrent spiral chromatography module , comprising:a first input port for receiving an input solution;a first mixer for mixing the input solution with a recycled solution from a second input port to produce a first mixed output;a stage I separator for concentrating the first mixed output to produce a stage I solid fraction, wherein a stage I liquid fraction exits the stage I separator via a first output port;a second mixer for mixing the stage I solid fraction from the stage I separator and an optional buffer solution from a third input port to produce a second mixed output;a stage II separator for concentrating the second mixed output to produce a stage II solid fraction which exits the module from the stage II separator via a second output port, wherein a stage II liquid fraction exits the module from the stage II separator via a third output port; andat least one pump,wherein the recycled solution from the third output port flows countercurrent to the input solution into the second input port, and at least one inlet;', 'a curvilinear channel arranged and disposed to generate ...

DEVICE AND METHOD FOR EXTRACTING SOLUBLE SUBSTANCES DISSOLVED IN AN AQUEOUS SOLUTION

A device for extracting soluble substances dissolved in an aqueous solution, the extraction device including a peripheral wall and an adsorbent agent contained in the peripheral wall, the adsorbent agent being capable of extracting at least one portion of the soluble substances by contact with the aqueous solution, the extraction device also including a maze, defining a circulation path for the aqueous solution within the peripheral wall, the maze including a main inlet and a main outlet of aqueous solution, in fluid communication with one another via the circulation path, the adsorbent agent being arranged inside the maze so as to be placed in contact with the aqueous solution during the circulation of the latter along the circulation path. 1. An extraction device for extracting soluble substances dissolved in an aqueous solution , the extraction device comprising:a peripheral wall and an adsorbent agent contained in this peripheral wall, the adsorbent agent being capable of extracting at least one portion of the soluble substances by contact with the aqueous solution; anda maze, defining a circulation path for the aqueous solution within the peripheral wall, the maze comprising a main inlet and a main outlet for the aqueous solution that are fluidly connected to one another by the circulation path, said adsorbent agent being arranged inside the maze so as to be placed in contact with the aqueous solution during the circulation of the latter along the circulation path.2. The extraction device claim 1 , wherein said maze further comprises a modular assembly of several removable modules claim 1 , each removable module being able to be assembled to claim 1 , or disassembled from claim 1 , the modular assembly in order to modify the circulation path.3. The extraction device according to claim 2 , wherein a first portion of the removable modules are wall modules claim 2 , together forming a portion at least of said peripheral wall when the wall modules are mounted ...

MULTI-DIMENSIONAL CHROMATOGRAPH SYSTEM

In a two-dimensional LC system configured to introduce components trapped in a trap column during a fractionation period T into a second-dimension column, separate components, and then detect the components using a mass spectrometer, a data collection unit receives a signal which indicates timing to delimit the fractionation period T, determines the first data item in the fractionation period T, adds measurement start point identification information to the first data item, and stores all the data in a data storage unit. A two-dimensional chromatogram creation unit recognizes the first data item in each fractionation period T from the read data, and create a two-dimensional chromatogram by aligning data so that the first data items will be aligned at the top along an abscissa. 1. A multi-dimensional chromatograph system comprising: an (n−1)th-dimension column (where n is an integer equal to or larger than 2) configured to chronologically separate a plurality of components contained in a sample; a holding unit configured to hold components of the sample , the components being obtained from the (n−1)th-dimension column within a predetermined time period; an nth-dimension column configured to further chronologically separate components of the sample held by the holding unit; and a detection unit configured to detect the components of the sample in sequence , the components being obtained from the nth-dimension column , wherein the components of the sample obtained from the (n−1)th-dimension column are fractionated at every predetermined time period and held by the holding unit , and an operation in which the components held by the holding unit are separated in the nth-dimension column and detected is performed repeatedly , the multi-dimensional chromatograph system further comprising:a) data collection means for collecting data items obtained in sequence by the detection unit and recording the data items by adding information to the data items or by associating the ...

MODULAR, INTEGRATED PROCESS AND APPARATUS FOR EXTRACTING, REFINING AND REMEDIATING ACTIVE SUBSTANCES FROM PLANT MATERIAL

The present disclosure relates to scalable processes for extracting, refining and remediating extracts of natural products, such as plant material and for providing well controlled refined extract. 1. A system for processing a biomass feed , comprising:a sequential simulated moving bed (SSMB) chromatography unit configured to (1) receive a first desorbent (D1), a second desorbent (D2), and one or more stream derived from said biomass feed, said one or more stream comprising one or more cannabinoid constituent and one or more constituent different from said one or more cannabinoid constituent and (2) generate from said one or more stream, with the aid of said D1 and said D2, a first stream comprising at least a portion of said one or more cannabinoid constituent and a second stream comprising at least a portion of said one or more constituent different from said one or more cannabinoid constituent.2. The system of claim 1 , wherein said D1 comprises ethanol and ethyl acetate and said D2 comprises water.3. The system of claim 1 , further comprising a processing unit in fluid connection with and operably coupled to said SSMB chromatography unit claim 1 , said processing unit configured to recycle said first stream claim 1 , said second stream claim 1 , or a combination thereof to provide a recycled phase of said D1 claim 1 , a recycled phase of said D2 claim 1 , or a combination thereof.4. The system of claim 1 , wherein said SSMB chromatography unit comprises less than or equal to 14 packed bed columns.5. The system of claim 4 , wherein an individual packed bed column of said packed bed columns comprises a reverse phase adsorbent.6. The system of claim 1 , wherein said SSMB chromatography unit comprises 6 or 8 packed bed columns.7. The system of claim 1 , wherein said SSMB chromatography unit is configured to generate and collect said first stream and said second stream for at least 1 day.8. The system of claim 1 , wherein said SSMB chromatography unit is configured ...

PURIFICATION METHOD AND USES THEREOF

A cyclic chromatographic purification method for the isolation of a product from a feed mixture consisting of the product and at least one further component representing impurities, which impurities bind stronger to the chromatographic stationary phase than the product is given. The method uses at least two chromatographic adsorbers as chromatographic stationary phase, grouped into only one first adsorber section () and one second adsorber section (), wherein if an adsorber section comprises more than one chromatographic adsorber these are permanently connected in series, wherein the first adsorber section () has a first adsorber section inlet and a first adsorber section outlet, and the second adsorber section () has a second adsorber section inlet and a second adsorber section outlet. 212. A method according to wherein each adsorber section ( claim 1 ,) consists of only one adsorber such the total number of adsorbers in the process is two.312112. A method according to any of the preceding claims claim 1 , wherein before for the first time carrying out the first interconnected step a start-up step (BSU) is carried out claim 1 , in which during a batch timespan (t) said adsorber sections ( claim 1 ,) are disconnected and an equilibrated adsorber section () to be taking the place of the first adsorber section () in the subsequent first interconnected step is loaded with feed mixture via the first adsorber section inlet and product is collected from first adsorber section outlet claim 1 , while the second adsorber section () is either being equilibrated or already equilibrated and inactive;4122. A method according to any of the preceding claims claim 1 , wherein after termination of the desired cycles of steps a.-f. the procedure is followed by a shut-down sequence (BSD) claim 1 , wherein during a first batch timespan (t) said adsorber sections ( claim 1 ,) are disconnected and the adsorber section having been subjected to washing in the preceding second batch step (B ...

METHOD FOR TREATING LIGNOCELLULOSIC MATERIALS

A method of generating a refined a sugar stream that comprises xylose from a biomass hydrolysis solution, including contacting a biomass hydrolysis solution that includes a population of mixed sugars comprising xylose, an acid, and impurities, with a thermally-phase separable solvent such as a glycol solvent to form an extraction mixture; and separating from said extraction mixture a first stream including the thermally-phase separable solvent, acid, and impurities and a second, refined sugar stream that comprises xylose. 1. An extraction mixture comprising:a biomass hydrolysis solution that comprises a population of mixed sugars comprising xylose, an acid, and impurities; anda thermally-phase separable solvent,wherein the extraction mixture is configured to separate into two phases with an increased temperature.2. The extraction mixture of claim 1 , wherein the thermally-phase separable solvent includes ethylene glycol claim 1 , propylene glycol claim 1 , or any combination thereof.3. The extraction mixture of claim 1 , further comprising an alkanol.4. The extraction mixture of claim 3 , wherein said alkanol is hexanol.5. The extraction mixture of claim 3 , wherein said alkanol is 2-ethylhexanol.6. The extraction mixture of claim 1 , wherein the extraction mixture comprises a 2-butoxyethanol.7. The extraction mixture of claim 1 , wherein the extraction mixture comprises a 1-propoxy-2-propanol.8. The extraction mixture of claim 1 , wherein the thermally-phase separable solvent is a glycol solvent. This application is divisional of U.S. application Ser. No. 15/548,166 filed on Aug. 2, 2017, which is a U.S. National Stage under 35 U.S.C. § 371 of International Application No. PCT/IB2016/050496, filed Feb. 1, 2016, which claims priority to Swedish Patent Application No. 1550109-1, filed Feb. 3, 2015, the entireties of which are incorporated herein by reference.Currently, sugar solutions are purified with extraction and/or chromatographic techniques or combinations ...

Method For Chromatographic Purification Of Viscous Loads

The invention relates to a method for purifying a mixture to be separated, in a multicolumn chromatography system, the method comprising successively and cyclically: a step of collecting a raffinate, a step of injecting the mixture to be separated, a step of collecting an extract and an eluent injection step, at an operating temperature; wherein the mixture to be separated has a viscosity at 20° C. greater than or equal to 3 mPa·s; and wherein the dry matter mass concentration of the mixture to be separated is equal, within 5%, to a threshold concentration, said threshold concentration is such that: the viscosity of the mixture to be treated at a dry matter mass concentration equal to the threshold concentration and at the operating temperature, is equal to twice the viscosity of the mixture to be treated, at a dry matter mass concentration equal to 85% of the threshold concentration and at the operating temperature.

CPC DISTRIBUTION CHROMATOGRAPHY OF CANNABINOIDS

The invention relates to cannabinoids and their isolation and purification and to obtaining them by means of centrifugal partition chromatography. 111.-. (canceled)12. A method for separating and/or purifying cannabinoids from a cannabis extract , including at least one liquid-liquid centrifugal partition chromatography step , wherein a solvent from the group of cyclohexane , heptane , or octane is selected and is kept stationary by centrifugal force , including and a second non-miscible liquid phase.13. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 12 , wherein the first phase is n-heptane.14. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 12 , wherein the second phase is acetonitrile claim 12 , methanol claim 12 , ethylacetate or water.15. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 12 , selected from the group consisting of:n-heptane/acetonitrile, n-heptane/ethylacetate/acetonitrile, n-heptane/ethylacetate/t-butyl methyl ether/acetonitrile,n-heptane/ethylacetate/methanol/water,n-heptane/methanol/water, n-heptane/ethanol/water,n-heptane/acetone/water, n-heptane/methanol/acetonitrile,n-heptane/ethanol/acetonitrile,n-heptane/acetone/acetonitrile,n-heptane/chloroform/acetonitrile,n-heptane/chloroform/methanol, n-heptane/methanol,n-heptane/ethanol/methanol,n-heptane/n-butanol/acetonitrile, n-heptane/2-propanol/water, n-heptane/n-propanol/water, n-heptane/2-propanol/acetonitrile, n-heptane/n-propanol/acetonitrile, n-heptane/dichloromethane/acetonitrile,n-heptane/dichloromethane/methanol,n-heptane/tetrahydrofuran/acetonitrile,n-heptane/benzotrifluoride/acetonitrile, Cyclohexane/methanol/water,Cyclohexane/methanol/acetonitrile,Cyclohexane/t-butyl methyl ether/water, Cyclohexane/acetonitrile/water, andIsooctane/methanol, isooctane/methanol/water, Isooctane/ethyl acetate/methanol/water.16. The method for separating ...

Xylene Separation Process and Apparatus

A simulated moving bed process using dual, parallel rotary valves configured or plumbed to be operated independently in which the step times of the rotary valves are staggered. In embodiments, the second rotary valve is programmed to step about halfway through the step time of the first rotary valve. Staggering the step time of the parallel rotary valves, rather than utilizing simultaneous stepping, results in increased net composite paraxylene concentration of the extract stream, allowing for increased capacity of the simulated moving bed process and/or reduced energy consumption. 114.-. (canceled)15. An apparatus for the continuous simulated countercurrent adsorptive separation of para-xylene comprising plural adsorptive separation chambers fluidly connected with plural bedlines Athrough A , wherein n>2 and n+j is the total number of bedlines , which in turn are fluidly connected with a matrix of conduits to distribute plural input streams , including desorbent , a primary flush input and a secondary flush input , and at least two multicomponent feeds differing in the concentration of para-xylene , to said plural bedlines , and plural output streams , including a primary flush output and a secondary flush output , an extract comprising a para-xylene-enriched product , and raffinate , from said plural adsorptive separation chambers , wherein the plural input streams and plural output streams are distributed by a first and second rotary valve in fluid communication with the conduits , the improvement comprising staggering the step time of the first rotary valve from the step time of the second rotary valve.16. The apparatus of claim 15 , wherein the second rotary valve steps about halfway through the step time of the first rotary valve.17. The apparatus of claim 15 , wherein the second rotary valve steps prior to about halfway through the step time of the first rotary valve.1810. The apparatus of claim 15 , wherein the second rotary valve steps within about seconds ...

PARALLEL ASSEMBLY OF CHROMATOGRAPHY COLUMN MODULES

A parallel assembly () of chromatography column modules (), the assembly having one common assembly inlet () and one common assembly outlet (), each column module comprising a bed space () filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space () of the column module with the assembly inlet () and the assembly outlet (), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly. 12115131353901555175729333583852915551757a,b,c;a,b,ca,b,ca, ba,ba,b;aiai. A parallel assembly (; ; ) of chromatography column modules (; , ) , the assembly having one common assembly inlet (; ) and one common assembly outlet (; ) , each chromatography column module comprising a bed space () filled with chromatography medium and each chromatography column module comprises integrated fluid conduits ( , - , -) which when the chromatography column module is connected with other chromatography column modules are adapted to connect the bed space () of the chromatography column module with the assembly inlet (; ) and the assembly outlet (; ) , wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.231353a,b,ca,b,ca,b,c. The parallel assembly of claim 1 , wherein said chromatography column modules (; ; ) are stacked together.3. The parallel assembly of claim 1 , wherein a compression force is applied to the chromatography column modules to achieve and/or secure fluid tight ...

PARALLEL MODULES FOR IN-LINE RECHARGING OF SORBENTS USING ALTERNATE DUTY CYCLES

Parallel modules for in-line recharging of sorbent materials using alternate duty cycles for a sorbent cartridge. The sorbent cartridge can have two or more modules contained therein having connectors connecting each of the modules. One or more of the modules can be reusable and the sorbent materials therein can be recharged. 1. (canceled)2. A method of recharging a sorbent , comprising the steps of:connecting at least a first module containing at least one sorbent material to a recharger; wherein the recharger contains at least one fluid capable of recharging the at least one sorbent material; andpassing the at least one fluid capable of recharging the at least one sorbent material through the first module.3. The method of claim 2 , wherein the first module is fluidly connected to at least a second module containing at least a second sorbent material.4. The method of claim 2 , wherein the first module contains zirconium phosphate claim 2 , and wherein the at least one fluid contains sodium and hydrogen ions.5. The method of claim 2 , wherein the first module contains zirconium oxide and wherein the at least one fluid contains sodium and hydrogen ions.6. The method of claim 2 , wherein the first module contains activated carbon claim 2 , and wherein the at least one fluid contains heated water.7. The method of claim 2 , wherein the first module contains alumina and urease claim 2 , and wherein the at least one fluid contains urease.8. The method of claim 3 , wherein one of the first and second modules contains zirconium phosphate and wherein the other one of the first and second modules contains zirconium oxide.9. The method of claim 3 , wherein at least one of the at least one fluids capable of recharging the at least one sorbent material is not passed through the second module.10. The method of claim 3 , wherein the first module and second module are connected in parallel.11. The method of claim 8 , further comprising the step of connecting the second module to a ...

CHROMATOGRAPHIC SEPARATION OF AMMONIUM SULFATE AND 2-HYDROXY-2-METHYLPROPIONIC ACID

The invention relates to a process for the chromatographic purification of a starting stream containing ammonium sulfate and 2-hydroxy-2-methylpropionic acid, comprising: 1. A process for the chromatographic purification of a starting stream containing ammonium sulfate and 2-hydroxy-2-methylpropionic acid , comprising:passing said starting stream through a bed of stationary phase;elution of a raffinate enriched in ammonium sulfate and depleted in 2-hydroxy-2-methylpropionic acid; andelution of an extract enriched in 2-hydroxy-2-methylpropionic acid and depleted in ammonium sulfate.2. The process as claimed in claim 1 , wherein the purification is performed by ion-exclusion chromatography.3. The process as claimed in claim 1 , wherein the stationary phase is a cationic resin.4. The process as claimed in claim 1 , wherein the stationary phase consists of particles with a size Dv50 of between 200 and 350 μm.5. The process as claimed in claim 1 , wherein the stationary phase is pre-equilibrated with a solution containing ammonium ions.6. The process as claimed in claim 1 , wherein the starting stream is at a pH of from 1 to 4 during its passage through the bed of stationary phase.7. The process as claimed in claim 1 , wherein an eluent is passed through the bed of stationary phase for elution of the raffinate and elution of the extract claim 1 , said eluent being demineralized water or acidified demineralized water claim 1 , at a pH of from 3 to 5 claim 1 , the acidified demineralized water having an acid content of from 0.0005 to 5 g/L.885. The process as claimed in claim 1 , which is performed at a temperature of from 20 to °.9. The process as claimed in claim 1 , performed on a multi-column chromatography system.1044. The process as claimed in claim 9 , wherein the chromatography system comprises a zone located between a raffinate collection line and an eluent injection line claim 9 , zone being traversed by a volume of mobile phase of between 0.3 and 0.55 BV.11. The ...

PARALLEL ASSEMBLY OF CHROMATOGRAPHY COLUMN MODULES

A parallel assembly () of chromatography column modules (), the assembly having one common assembly inlet () and one common assembly outlet (), each column module comprising a bed space () filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space () of the column module with the assembly inlet () and the assembly outlet (), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly. 1. A parallel assembly of chromatography column modules , the assembly having one common assembly inlet connected to a first end plate and one common assembly outlet connected to a second end plate , wherein the first and second end plates have no direct fluid contact with each other , a bed space filled with chromatography medium; and', 'integrated fluid conduits comprising a first integrated fluid conduit including a first tube fluid conduit connecting with the common assembly inlet and integrally combined with a first fluid conduit substantially perpendicular to the first tube fluid conduit and connected to an inlet of the bed space at a first end of the bed space to form a first distribution system, and a second integrated fluid conduit including a second tube fluid conduit connected with the common assembly outlet and integrally combined with a second fluid conduit substantially perpendicular to the second tube fluid conduit and connected to an outlet of the bed space at a top of the bed space to form a second distribution system,', 'wherein when said chromatography column modules are connected together the first and second integrated fluid conduits of each respective module are configured to connect their ...

MODULAR AUTOMATED CHROMATOGRAPHY SYSTEM

Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform may recognize the modules and their locations, coordinate fluid connections between the modules, and provide a variety of control, monitoring, data generating and data processing functions to generate chromatographic data. The software platform may also provide graphical tools for designing chromatographic methods from a library of phases. 1. A method of operating a chromatography system , the method comprising:receiving a plurality of modules into a mounting frame, wherein each of the plurality of modules comprises a fluid manipulation component and a microcontroller that transmits operational signals to the fluid manipulation component in response to commands received from outside the module, and wherein the mounting frame further includes a plurality of bays, each of the plurality of bays constructed to receive a single module, and each of the plurality of bays comprising a signal connector that couples to and communicates with the microcontroller of a module mounted in the bay, wherein at least some of the bays are constructed to receive single modules interchangeably with other modules, and wherein the fluid manipulation components are connected together by fluid lines in an arrangement for implementing a flow scheme;displaying a depiction of the flow scheme in the user interface, wherein the depiction of the flow scheme is arranged generally linearly with flow from left to right, regardless of the physical ...

METHODS FOR THE PURIFICATION OF PROTEINS USING CAPRYLIC ACID

A protein purification process with virus inactivation or removal uses caprylic acid (octanoic acid) at acidic pH. The method comprises caprylic acid treatment as part of the chromatographic step so as to perform viral inactivation without a discontinuous process and without the requirement of attention by personnel, particularly when the pH adjustment of the eluate is performed automatically by eluting into buffer. The method of the invention further results in mycoplasmas being inactivated, and reduced impurities like Host Cell Protein (HCP). 1. A method for the purification of a target protein from a sample comprising the steps ofi. loading a target protein-containing sample onto a stationary phase, so as to bind the target protein to said stationary phase;ii. subjecting the solid phase with the bound target protein to a caprylic acid solution; wherein the caprylic acid solution is at a pH so as to form free caprylic acid, and wherein the caprylic acid solution is a caprylic acid buffer at a concentration of 1 to 50 mM, as measured in terms of free caprylic acid; andiii. eluting the target protein.2. The method according to claim 1 , wherein purification of a target protein comprises inactivation of virus in said sample.3. A method for the preparation of a virus-free protein solution claim 1 , comprising the steps ofi. loading a target protein-containing sample onto a stationary phase, so as to bind the target protein to said stationary phase;ii. subjecting the solid phase with the bound target protein to a caprylic acid solution; wherein the caprylic acid solution is at a pH so as to form free caprylic acid, and wherein the caprylic acid solution is a caprylic acid buffer at a concentration of 1 to 50 mM, as measured in terms of free caprylic acid; andiii. eluting the target protein.4. The method according to claim 1 , wherein the caprylic acid solution comprises a combination i) a caprylate salt and ii) an inorganic or organic acid in proportions so provide a ...

Separation Method

The invention relates to a method of isolating an immunoglobulin, comprising the steps of: 1. A method of isolating an immunoglobulin , comprising the steps of:a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein said porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml;b) contacting a liquid sample comprising an immunoglobulin with said separation matrix;c) washing said separation matrix with a washing liquid;d) eluting the immunoglobulin from the separation matrix with an elution liquid; ande) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.2. The method of claim 1 , wherein said cross-linked polymer particles have a pore size corresponding to an inverse gel filtration chromatography Kd value of 0.69-0.85 for dextran of Mw 110 kDa.3. The method of claim 1 , wherein said separation matrix has a max pressure of at least 0.58 claim 1 , such as at least 0.60 claim 1 , MPa when packed at 300 +/−10 mm bed height in a FineLine™ 35 column.4. The method of claim 1 , wherein said immunoglobulin comprises an Fc fusion protein.5. The method of claim 1 , wherein said immunoglobulin has a hydrodynamic radius of at least 6.0 nm.6. The method of claim 1 , wherein said immunoglobulin comprises a bispecific claim 1 , trispecific or polyspecific antibody.7. The method of claim 6 , wherein said method separates half antibodies or homodimeric antibodies from said bispecific claim 6 , trispecific or polyspecific antibody.8. The method of claim 1 , wherein said immunoglobulin comprises a conjugated antibody.9. The method of claim 1 , wherein said immunoglobulin comprises an antibody fragment.10. The method of claim 1 , wherein the separation matrix is Mabselect™ PrismA.11. A method of isolating an immunoglobulin ...

RAPID SOLID PHASE EXTRACTION DEVICE AND METHODS

A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body. 1. A method for extracting a compound of interest from a sample matrix , comprising the steps of:a. drawing a sample matrix into a syringe having a plunger;b. contacting the sample with a sorbent to bind the compound of interest to the sorbent;c. drawing elution buffer into the syringe containing the sorbent;d. expressing the elution buffer containing the compound of interest through a desalting column.2. The method of claim 1 , further comprising the step of mixing the sample matrix with one or more buffer.3. The method of claim 2 , wherein the syringe is prefilled with one or more buffer solutions before the sample is drawn into the syringe.4. The method of claim 2 , wherein at least one buffer solution is placed in the syringe before the sample matrix is drawn into the syringe.5. The method of claim 2 , wherein at least one buffer solution is placed in the syringe after the sample matrix is drawn into the syringe.6. The method of wherein said one or more buffer comprises a plurality of buffers.7. The method of wherein at least two of the plurality of buffers are placed into the syringe sequentially.8. The method of wherein at least two of the plurality of buffers are placed into the syringe premixed.9. The method of claim 2 , wherein at least one buffer is a lysis buffer.10. The method of further comprising the step of allowing the sample matrix and buffer mixture to incubate for a suitable period of time.11. The method of wherein the sorbent comprises at least one material selected from the group consisting of: silica claim 1 , acid-washed silica claim 1 , glass beads claim 1 , acid-washed glass beads claim 1 , zeolite claim 1 , silica gel claim 1 , filters embedded with silica particles ...

A novel affinity matrix and devices for isolation and purification of rna and dna for point of care molecular devices

The present disclosure relates to nucleic acid extraction and purification methods and devices to accomplish the same. The present disclosure proposes a novel approach to this problem wherein cell isolation and nucleic acid purification can be integrated in a single “step,” by using the same solid phase for both cell adsorption and nucleic acid purification. This is achieved by binding the cells to a solid support as a first step. The same solid support is then used under conditions that lyse the bound cells, and then subsequently enable the nucleic acid to bind to the support. Methods of the present disclosure relate to the isolation of nucleic acid, and especially to a method for isolating DNA from cells, biological or environmental samples using antibiotics, which bind nucleic acids.

ISOIDIDE MANUFACTURE AND PURIFICATION

Methods are provided for the purification of isoidide. The methods comprise subjecting an at least partially deionized isoidide composition comprising isoidide and other isohexides to chromatographic separation of isoidide from other isohexides, removal of solvent from the isoidide, and crystallization of the isoidide. Isoidide of monomer-grade purity is obtained. 1. A method of preparing an isoidide enriched composition comprising , at least partially removing ionic species from an epimerization product comprising isoidide to obtain an at least partially deionized isoidide composition comprising isoidide and other isohexides; and ,chromatographically separating the isoidide from other isohexides to obtain a stream enriched in isoidide and a stream enriched in the other isohexides.2. The method of claim 1 , wherein the chromatographic separation is carried out by contacting the at least partially deionized isoidide composition with a resin comprising strong acid ion exchange resin in the calcium 2 form;contacting the at least partially deionized isoidide composition comprising isoidide and other isohexides and the resin with a solvent; and,eluting a stream enriched in isoidide and a second stream enriched in the other isohexides.3. The method according to claim 1 , wherein the amount of ions in the at least partially deionized isoidide composition is below detection limits as determined by inductively coupled plasma atomic emission spectroscopy.4. The method according to claim 2 , wherein the resin is contained on chromatographic beds claim 2 , columns or parts thereof arranged in a simulated moving bed chromatographic array.5. The method according to claim 1 , wherein the solvent comprises water.6. A method according to claim 1 , wherein upon removing solvent the stream enriched in isoidide claim 1 , the proportion of isoidide in the stream enriched in isoidide is greater than 40 claim 1 , 45 claim 1 , 50 claim 1 , 55 claim 1 , 60 claim 1 , 65 claim 1 , 70 claim 1 ...

HIGH EFFICIENCY CONTINUOUS COUNTERCURRENT TANGENTIAL CHROMATOGRAPHY

A system, module and method for continuous or batch single-pass countercurrent tangential chromatography are disclosed for bind/elute and negative chromatography applications. The system includes binding, washing, elution (for bind/elute), regeneration, and equilibration single-pass modules. The resin slurry flows in a continuous single pass at steady-state through each module, while corresponding buffers flow countercurrent to the slurry facilitating efficient product and impurity extraction. The module and system include retentate pumps for better process robustness and control. A resin tank configured to be reversibly isolated from the single-pass modules facilitates a closed and disposable system. The method includes receiving unpurified product solution and resin slurry, isolating the resin tank, binding product (bind/elute) or impurities (negative) to the resin slurry, washing impurities from the resin slurry, eluting and capturing pure product from the resin slurry (bind/elute), regenerating the resin slurry following elution, and providing buffer solutions to all of the single-pass steps. 1. A system for continuous , single-pass countercurrent tangential chromatography having multiple single-pass modules , comprising:a single-pass binding step module for binding product from an unpurified product solution with a resin slurry;a single-pass washing step module for washing impurities from the resin slurry;a single-pass elution step module for eluting an output of the washing stage module as purified product solution;a single-pass regeneration step module for regenerating the resin slurry;a single-pass equilibration step module for equilibrating the resin slurry; anda resin tank for containing the resin slurry, the resin tank being configured to be reversibly isolated from the multiple single-pass modules following discharge of the resin slurry from the resin tank into the multiple single-pass modules,{'b': '112', 'wherein each single-pass module includes at ...

METHOD FOR PURIFYING SOLID-PHASE SYNTHETIC CRUDE LIRAGLUTIDE

The present invention relates to the field of biomedicine, and in particular, to a method for purifying solid-phase synthetic crude liraglutide. The method comprises: dissolving solid-phase synthetic crude liraglutide in an aqueous acetonitrile solution to obtain a crude peptide solution; and obtaining liraglutide with high purity and high yield through four-step HPLC purification. 1. A method for purifying crude liraglutide obtained from solid-phase synthesis , which is characterized by comprising the following steps:(a) a solution of crude liraglutide is obtained by dissolving crude liraglutide obtained from solid-phase synthesis in aqueous acetonitrile solution;(b) the solution of crude liraglutide is subjected to a first HPLC purification using octylsilane bonded silica as stationary phase, and using aqueous isopropanol solution containing 0.1-0.2% trifluoroacetic acid as mobile phase A and acetonitrile containing 0.1-0.2% trifluoroacetic acid as mobile phase B eluting at a linear gradient from 20-40% B to 40-60% B, and target peak is collected as the first fraction;(c) the first fraction is subjected to a second HPLC purification using cyanosilane bonded silica as stationary phase, and using 0.05-0.15% (mass concentration) aqueous perchloric acid solution as mobile phase A and 0.05-0.15% (mass concentration) perchloric acid in acetonitrile as mobile phase B eluting at a linear gradient from 40% B to 70% B, and target peak is collected as the second fraction;(d) the second fraction is subjected to a third HPLC purification using octylsilane bonded silica as stationary phase, and using 0.01-0.06% (mass concentration) aqueous ammonia solution as mobile phase A and acetonitrile of chromatographic grade as mobile phase B eluting at a linear gradient from 30% B to 60% B, and target peak is collected as the third fraction; and(e) purified liraglutide is obtained from the third fraction by rotatory evaporation under reduced presser and lyophilization.2. The ...

Multi-dimensional liquid chromotography with second dimension having a variable flow rate

A multi-dimensional liquid chromatography system includes first and second liquid chromatography systems. The first system is configured for providing a first chromatographic separation of a sample fluid comprised in a first mobile phase and to provide a first effluent including at least a portion of the separated sample fluid. The second system is configured for providing a second chromatographic separation of at least a portion of the first effluent comprised in a second mobile phase. A control unit is configured to operate the first liquid chromatography system by maintaining a first flow rate of the first mobile phase substantially constant during the first chromatographic separation, and to operate the second liquid chromatography system during the second chromatographic separation according to a control value different from the second flow rate, so that a variation in the control value can lead to a variation in the second flow rate.

Elevated multi-stage chilled filter system

Refrigerated solvent is fed through a cooling jacket around an essential element extraction vessel. After circulating through the cooling jacket, the solvent is re-chilled and at least some of the solvent is passed into the extraction vessel, in which essential elements dissolve into the solvent. Downstream of the extraction vessel, after adsorbent media treatment, the extracted oil and solvent mixture is filtered, in a chilled state, through one or more filtration units. A filtration unit may be a system of vertically oriented filters of decreasing pore size sealed and insulated from the atmosphere. Pressurized gas is used to force the oil and solvent through the filters. Each filter stage has a removable lid, which provides convenient access for replacing the filter cartridge without disturbing the thermally insulated sidewalls of the filter stage.

METHOD FOR PRODUCING PURIFIED STEVIOL PRODUCT USING SIMULATED MOVING BED CHROMATOGRAPHY

Disclosed is a continuous process for the purification of steviol glycosides such as Rebaudioside D and/or Rebaudioside M extracted from the dried leaves or extracted from a fermentation broth using continuous simulated moving bed processes and nanofiltration without the addition of organic solvents to obtain a purified steviol product comprising sweet steviol glycosides. The sweet steviol glycosides can be used as substitutes for caloric sweeteners in beverages and in other food items. 1. A method of purifying one or more steviol glycosides from a mixture , the mixture including the one or more steviol glycosides and at least one impurity , the method comprising:passing the mixture through a first adsorbent with a first solvent, the first adsorbent comprising one or more hydrophobic interaction resins or one or more ion exchange resins to provide a first eluate stream, the first eluate stream having the first solvent and a higher purity of the one or more steviol glycosides than in the mixture as measured by weight percentage of the solid content, andoptionally removing at least a portion of the first solvent from the first eluate stream to provide a reduced first eluate stream.2. The method of claim 1 , the method further comprising:passing the first eluate stream or the reduced first eluate stream through a second adsorbent with a second solvent, the second adsorbent comprising one or more hydrophobic interaction resins or one or more ion exchange resins to provide a second eluate stream, the second eluate stream having the second solvent and a higher purity of the one or more steviol glycosides than in the first eluate stream or the reduced first eluate stream as measured by weight percentage of the solid content, andoptionally removing at least a portion of the second solvent from the second eluate stream to provide a reduced second eluate stream.3. The method of claim 1 , wherein the one or more steviol glycosides are selected from the group consisting of ...

METHOD FOR PREPARING NATURAL L-CYSTEINE CRYSTALS BY CONTINUOUS CHROMATOGRAPHY

The present disclosure relates to a method for preparing L-cysteine crystals, and L-cysteine crystals prepared by the method. Through the method for preparing L-cysteine crystals of the present disclosure, L-cysteine crystals can be obtained from a natural L-cysteine fermentation broth with a high recovery rate and/or purity without a chemical reaction or the use of an artificial synthetic compound. 1: A method for preparing L-cysteine crystals , comprising:(a) obtaining a separated liquid after introducing a fermentation broth in a pH range of 3.0 to 9.0 containing L-cysteine into a continuous chromatography apparatus having a strongly acidic cation-exchange resin as a stationary phase;(b) concentrating the separated liquid; and(c) recovering L-cysteine crystals from the concentrate.2: The method of claim 1 , further comprising adjusting the fermentation broth containing L-cysteine to a pH of 3.5 to 7.5 prior to step (a).3: The method of claim 1 , further comprising concentrating the fermentation broth in a pH range of 3.0 to 9.0 containing L-cysteine prior to step (a).4: The method of claim 1 , wherein the strongly acidic cation-exchange resin in step (a) has a sulfuric acid functional group.5: The method of claim 1 , wherein the strongly acidic cation-exchange resin in step (a) is a styrene-divinylbenzene copolymer having a sulfuric acid functional group.6: The method of claim 1 , wherein the continuous chromatography apparatus in step (a) is a simulated moving bed (SMB) chromatography apparatus.7: The method of claim 1 , wherein the separated liquid in step (a) has a solid content of L-cysteine excluding moisture of 85% (w/w) or more.8: The method of claim 1 , wherein the yield of continuous chromatography in step (a) claim 1 , as a ratio of L-cysteine in the separated liquid obtained relative to the fermentation broth introduced claim 1 , is 50% (w/w).9: The method of claim 1 , wherein step (b) is carried out such that the concentration of L-cysteine in the ...

Process and Apparatus for the Production of Para-xylene

The present invention is an improved process and apparatus for producing para-xylene, particularly with respect to a process that involves the methylation of toluene and/or benzene to selectively produce para-xylene, wherein streams having differing amounts of ethylbenzene are separately treated in the recovery of para-xylene. A first hydrocarbon feed comprising xylenes and ethylbenzene is provided to a first para-xylene adsorption section, and a second hydrocarbon feed comprising xylenes and less EB than the first hydrocarbon feed is provided to a second para-xylene adsorption section. Segregating the feeds with differing ethylbenzene contents increases the overall efficiency of the adsorption of para-xylene by the adsorption units. Efficiency and energy savings may be further improved by subjecting the lower-content ethylbenzene stream to liquid phase isomerization. 115.-. (canceled)16. An apparatus for the production of para-xylene comprising:a first para-xylene adsorption section, which produces a first para-xylene-rich stream and a first para-xylene-depleted stream from a first hydrocarbon feed, and a second para-xylene adsorption section, which produces a second para-xylene-rich stream and a second para-xylene-depleted stream from a second hydrocarbon feed, the first and second para-xylene adsorption sections fluidly connected to a divided wall raffinate column in which the first and second para-xylene-depleted streams are provided to opposite sides of the dividing wall and separated into an ethylbenzene-rich stream, which contains a majority portion of the ethylbenzene from the first and second para-xylene-depleted streams, and an ethylbenzene-depleted stream, which contains a minor portion of the ethylbenzene from the first and second para-xylene-depleted streams;a vapor phase isomerization unit fluidly connected to the divided wall raffinate column to isomerize meta-xylene, ortho-xylene, and ethylbenzene in the ethylbenzene-rich stream at least partially in ...

TECHNOLOGY FOR EXTRACTING AND PREPARING HIGH-PURITY RAFFINOSE FROM DEFATTED WHEAT GERM

The present invention discloses a process for preparing high-purity raffinose from defatted wheat germ comprising the steps of percolate extraction of raffinose from defatted wheat germ, decoloration by extraction from the abstraction liquid, electrodialysis desalination, impurity removal by simulated moving bed, concentration and crystallization, with the absolute purity of raffinose as high as 98% and the recovery up to 75%. The process is not only reliable and easy to operate, but also easy to realize industrial production and control the parameters. 1. A process for preparing high-purity raffinose from defatted wheat germ , the method comprising the follows steps:(1) conducting percolation extraction of the defatted wheat germ, and collecting percolate containing raffinose;(2) concentrating percolate of Step (1) to remove alcohol, dissolving solid substance followed by filtering to insoluble substance, extracting the filtrate with an organic solvent and concentrating the aqueous phase, obtaining a decolored solution;(3) processing the decolored solution of Step (2) with a microporous membrane of a drainage, diluting the solution with water to obtain a pretreatment liquid with 50˜150 mg/mL solid concentration, and desalinating the pretreatment liquid with electrodialysis to obtain a desalination solution;(4) separating the desalination solution of Step (3) with a simulated moving bed, and collecting flow containing raffinose, obtaining a supersaturated syrup by concentrating; and(5) crystallizing the supersaturated syrup and obtaining white crystallization raffinose after drying.2. The process for preparing high-purity raffinose from defatted wheat germ claim 1 , according to claim 1 , characterized in that the volume of solution with dissolving solid substance of step (2) is 20%˜35% of the percolate volume.3. The process for preparing high-purity raffinose from defatted wheat germ claim 1 , according to claim 1 , characterized in that organic solvent of Step (2) ...

METHODS OF PROCESSING A FLUID INCLUDING A RECOMBINANT THERAPEUTIC PROTEIN AND USE THEREOF

Provided herein are methods of processing a fluid that include the use of one or both of a circuit system including a tangential flow filtration (TFF) unit and a circuit system including a tangential flow virus filtration (TFVF) unit. Also provided are integrated and continuous processes for manufacturing a therapeutic protein drug substance that include a step of processing a fluid including the recombinant therapeutic protein using any of the methods provided herein. 1. A method of processing a fluid comprising a recombinant therapeutic protein , the method comprising:(a) providing a circuit system comprising (i) a tangential flow filtration (TFF) unit having first and second inlets, and (ii) a conduit in fluid communication between the first and second inlets, comprising at least one port for flowing fluid into or out of, or both, of the system, wherein the system is configured such that fluid can be flowed through the conduit and the TFF unit, and filtrate not comprising the recombinant therapeutic protein can be collected from the TFF unit;(b) continuously flowing a fluid comprising a recombinant therapeutic protein into the circuit system through one of the at least one port, and discarding filtrate not comprising the recombinant therapeutic protein for a first period of time;(c) continuously flowing a diafiltration medium into the circuit system through one of the at least one port, and discarding filtrate not comprising the recombinant therapeutic protein for a second period of time; and(d) collecting a fluid comprising the recombinant therapeutic protein that is present in the circuit system after the first and second time periods through one of the at least one port.2. The method of claim 1 , wherein step (b) comprises continuously flowing the fluid into the circuit system at a rate of about 0.1 mL/minute to about 100 L/minute.35.-. (canceled)6. The method of claim 1 , wherein the flowing of the fluid into the circuit system in step (b) occurs ...

HIGH-FLOW FLUID VALVE BLOCK

An illustrative valve block includes a plate, a fluid transfer block, and a diaphragm. The plate includes a channel configured to receive a first fluid and a recess connected to the channel. The fluid transfer block includes an inlet connection configured to receive a second fluid and an outlet connection. The fluid transfer block also includes a plurality of valve inlet bores connected to the inlet connection. The plurality of valve inlet bores are distributed along at least part of a first curved shape. The fluid transfer block further includes a plurality of valve outlet bores each fluidly connected to the outlet connection. The plurality of valve outlet bores are distributed along at least part of a second curved shape. The diaphragm is between the pressure plate and the fluid transfer block. The plurality of valve inlet bores and the plurality of valve outlet bores adjoin the recess. 1. A valve block comprising: a channel configured to receive a first fluid; and', 'a recess connected to the channel;, 'a plate comprising an inlet connection configured to receive a second fluid;', 'an outlet connection;', 'a plurality of valve inlet bores connected to the inlet connection, wherein the plurality of valve inlet bores is distributed along at least part of a first shape; and', 'a plurality of valve outlet bores connected to the outlet connection, wherein the plurality of valve outlet bores is distributed along at least part of a second shape, wherein one of the first or second shapes is within the other of the first or second shapes; and, 'a fluid transfer block comprisinga diaphragm between the plate and the fluid transfer block, wherein the plurality of valve inlet bores and the plurality of valve outlet bores adjoin the recess.2. The valve block of claim 1 , wherein:in a first state in which a pressure within the recess is above a threshold pressure, the diaphragm is pressed against a surface of the fluid transfer block in which the plurality of valve inlet bores ...

DIRECT CAPTURE USING LARGE BEAD CHROMATOGRAPHY MEDIA

Disclosed is a continuous process in which a subset of a number of mutually identical columns, are connected in series. The process liquid, e.g. crude cell culture harvest, is supplied to the most upstream column of the subset. It flows successively through the in series connected columns and leaves the subset through the most downstream and flows into the downstream collection vessel. As soon as the packed bed of the most upstream column is become saturated with product, this column is disconnected from the subset. It is removed from the series connection. A replacement, identical, column is added such that it is connected in series downstream from the most downstream column of the subset. This process is repeated. 115-. (canceled)16. Method of processing a process liquid using a set of mutually identical liquid chromatography columns , each column is for liquid chromatography comprising a torus shaped packed bed of beads , the process liquid containing biologics that are captured by the beads wherein as the first processing step downstream from the process liquid creating source , being a cell culture vessel , the process liquid is fed to a subset of at least two of the set of identical columns directly from the source without any intermediate filtration or other clarification equipment , nor any harvest holding-step or holding vessel and the process liquid , depleted by the subset of columns from the product of interest , is leaving said subset of columns after having flown radially through each of the columns of the subset of columns ,whereinthe process liquid is concentrated crude CHO cell culture harvest, the CHO cell diameter is approximately 0,1-10% of bead diameter, the bead diameter is approximately 120-500 micrometer, the beads are hydrophilic;each liquid chromatography column of the set is designed to be radially flown through by the process liquid and comprising the packed bed of beads designed to capture product from the process liquid, the packed bed ...

Perfusion Bioreactor With Filtration Systems

The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.

PROTEIN PURIFICATION DEVICE

A device for purifying protein from a protein-containing fluid includes a prefilter unit, which has at least one size exclusion filter, a membrane adsorber unit, which has at least one porous membrane with functional constituents which adsorb the protein to be purified, and a collecting container. The collecting container, the membrane adsorber unit and the prefilter unit are configured such that the collecting container is suitable for receiving the membrane adsorber unit; and the membrane adsorber unit is suitable for receiving the prefilter unit. An associated method for purifying protein from a protein-containing fluid using the device is also disclosed. 1. A device for purifying protein from a protein-containing fluid , comprising:a cylindrically shaped prefilter unit, which has at least one size exclusion filter;a cylindrically shaped membrane adsorber unit, which has at least one porous membrane with functional constituents that adsorb the protein to be purified; anda cylindrically shaped collecting container;wherein the collecting container is suitable for receiving the membrane adsorber unit and the membrane adsorber unit is suitable for receiving the prefilter unit.2. The device of claim 1 , wherein the prefilter unit is suitable for receiving about 0.5 to about 25 ml of the fluid claim 1 , and the collecting container is suitable for receiving about 1 to about 50 ml of the fluid.3. The device of claim 1 , wherein the prefilter comprises three filters claim 1 , including an upper glass fiber filter having a pore size ranging from about 1.5 μm to about 3.25 μm claim 1 , a middle glass fiber filter having a pore size ranging from about 0.8 μm to about 1.4 μm claim 1 , and a lower asymmetric PES-membrane filter having a pore size of about 0.22 μm.4. The device of claim 1 , wherein the size exclusion filter is arranged at one end of the cylindrically shaped prefilter unit.5. The device of claim 1 , wherein the functional constituents of the porous membrane of ...

Recovery and precipitation of various elements and compounds

Systems and methods are disclosed for extracting a plurality of materials from a solution. These include a plurality of extraction devices. The extraction devices use a resin suspended above at least one screen, and the resin is used to extract at least one material from a fluid. A liquid is forced through the plurality of extraction devices and a separate material is extracted in each of the extraction devices. The resin is selected for each of the extraction devices and is based upon the material for which that extraction device is designed to remove from the fluid. Each of the extraction devices operate in series to remove at least one material from the fluid.

HIGH-FLOW FLUID VALVE BLOCK

An illustrative valve block includes a plate, a fluid transfer block, and a diaphragm. The plate includes a channel configured to receive a first fluid and a recess connected to the channel. The fluid transfer block includes an inlet connection configured to receive a second fluid and an outlet connection. The fluid transfer block also includes a plurality of valve inlet bores connected to the inlet connection. The plurality of valve inlet bores are distributed along at least part of a first curved shape. The fluid transfer block further includes a plurality of valve outlet bores each fluidly connected to the outlet connection. The plurality of valve outlet bores are distributed along at least part of a second curved shape. The diaphragm is between the pressure plate and the fluid transfer block. The plurality of valve inlet bores and the plurality of valve outlet bores adjoin the recess. 1. A valve block comprising:an inlet connection configured to receive a first fluid;an outlet connection;a plurality of valve inlet bores connected to the inlet connection, wherein the plurality of valve inlet bores is distributed along at least part of a first shape;a plurality of valve outlet bores connected to the outlet connection, wherein the plurality of valve outlet bores is distributed along at least part of a second shape, and wherein one of the first or second shapes is within the other of the first or second shapes; anda diaphragm configured to control flow of the first fluid from the plurality of inlet bores to the plurality of outlet bores in response to application of a pressurized material.2. The valve block of claim 1 , further comprising a plate including:a channel configured to receive a first fluid; anda recess connected to the channel.3. The valve block of claim 2 , further comprising a fluid transfer block claim 2 , wherein the fluid transfer block comprises the inlet connection claim 2 , the outlet connection claim 2 , the plurality valve inlet bores claim 2 , ...

Method for Adapting UV Cell Pathlength in a Chromatography System

The present invention relates to a method for determining operational status of a chromatography column (1; 39, 47, 59; 107, 109, 111, 113), comprising detecting a feed signal (21; 201) representative of the composition of a feed material provided to the inlet of the column; detecting the UV absorbance in the feed material, detecting an effluent signal (23; 203, 205, 207, 209) representative of the composition of the effluent from the column; and using the feed signal and the effluent signal to determine operational status of the column. The feed signal is generated using a first UV detector having a first UV cell pathlength operating at a first UV wavelength and in the effluent signal is generated using a second UV detector having a second UV cell pathlength operating at a second UV wavelength. The method further comprising determining a first threshold value based on the detected UV absorbance in the feed material, and selecting the first UV cell pathlength and/or first UV wavelength based on the first threshold value.

Alternating Flow Column Chromatography Apparatus and Method of Use

An alternating flow column chromatography apparatus comprising a ‘U’ shaped or T shaped separation column including at least one loading port for loading of components for separation, a first purification column in fluid communication with one end of the separation column and a second purification column in fluid communication with another end of the separation column, at least one eluent input port, an eluate output port and an alternating flow valve in fluid communication with the primary eluent input port, the eluate output port, the first purification column and the second purification column wherein, when operated, the alternating flow valve reverses the flow of eluent through the purification columns and the separation column. Also a method of using the apparatus. A benefit of the apparatus and method is more efficient operation compared to existing direct flow column chromatography apparatuses. 1. An alternating flow column chromatography apparatus for chromatographic separation and chromatographic purification of ionic radionuclide components and chemical components soluble in a solution comprising:a chromatographic separation column of ‘U’ shape or modified ‘I’ shape, including at least one loading port for loading of components for separation;a first purification column in fluid communication with one end of the separation column and a second purification column in fluid communication with another end of the separation column;at least one eluent input port;an eluate output port; andan alternating flow valve in fluid communication with the at least one eluent input port, the eluate output port, the first purification column and the second purification column wherein, when operated, the alternating flow valve reverses the flow of eluent through the purification columns and the separation column.2. The apparatus of wherein one of the at least one eluent input port is a primary eluent input port and there are one or more specific eluent input ports.3. The ...

ION EXCHANGER

An ion exchanger includes a case having an inflow hole and an outflow hole. The case accommodates a tube. A first passage is defined between the case and the tube. A second passage is defined in the tube. A first end of the first passage and a first end of the second passage are connected to each other. The first passage includes a lower portion defining a lower accommodation portion that is filled with an anion exchange resin. The first passage includes an upper accommodation portion located above the lower accommodation portion. The upper accommodation portion is filled with a cation exchange resin. The upper accommodation portion has a smaller volume and a smaller refrigerant flow area than the lower accommodation portion. 1. An ion exchanger comprising:a case including an inflow hole into which a refrigerant flows and an outflow hole out of which the refrigerant flows; andan ion exchange resin arranged in the case to remove ions from the refrigerant, whereinthe inflow hole and the outflow hole are located in one of an upper portion and a lower portion of the case,the case accommodates a tube extending in a vertical direction,a first passage is defined between an inner wall of the case and an outer wall of the tube,a second passage is defined in the tube,the first passage and the second passage each include a first end and a second end,the first end of the first passage and the first end of the second passage are connected to each other,the second end of the first passage and the second end of the second passage are not connected to each other,one of the second end of the first passage and the second end of the second passage is connected to the inflow hole and the other one of the second end of the first passage and the second end of the second passage is connected to the outflow hole,the first passage includes a lower portion defining a lower accommodation portion that is filled with an anion exchange resin serving as the ion exchange resin,the first passage ...

Process for purifying a hydrocarbon feed

A process for purifying a hydrocarbon feed, using a first adsorption unit with first and second adsorption columns respectively filled with first and second adsorbent solids by simultaneously: a) treating the liquid phase hydrocarbon feed in the first adsorption column by contact with the first adsorbent solid to adsorb at least a portion of impurities present and to produce hydrocarbon effluent which is depleted in impurities; b) treating a secondary liquid hydrocarbon feed constituted either by a fraction of the hydrocarbon feed or by a fraction of the hydrocarbon effluent and depleted in impurities to purify the secondary liquid hydrocarbon feed; c) heating the treated secondary liquid hydrocarbon feed from step b); d) regenerating the second adsorbent solid of the second adsorption column which comprises impurities with the secondary hydrocarbon feed heated in step c) to desorb the impurities to produce an effluent with impurities.

PURIFICATION OF LONG CHAIN DIACIDS

The present disclosure relates to methods for separating and purifying a long chain diacid from other long chain diacids, monocarboxylic acids, hydroxyl acids or alkanes by simulated or actual moving bed chromatography. 1. A method for separating a C6- to C18-carbon diacid from at least one impurity in a solution , comprising:(a) introducing a feed stream comprising a solution comprising at least one 6- to 18-carbon diacid and at least one impurity, the at least one impurity comprising a component more polar than the diacid, a component less polar than the diacid, or both, into a first zone of moving bed chromatography apparatus (MBCA) having one or more zones;(b) collecting a raffinate stream or an extract stream from the first zone of the MBCA, the raffinate stream comprising the diacid and one or more components more polar than the diacid, and the extract stream comprising the diacid and one or more components less polar than the diacid;(c) introducing the raffinate stream or the extract stream into a second zone of the MBCA;(d) collecting a second raffinate stream or a second extract stream from the second zone of the MBCA, the raffinate stream comprising the diacid and components more polar than the diacid, and the extract stream comprising the diacid and components less polar than the diacid;(e) introducing the second raffinate stream or the second extract stream into the first zone or the second zone of the MBCA;(f) optionally repeating steps (d) and (e) until a desired degree of separation is achieved; and(g) collecting a final raffinate stream or a final extract stream from a zone of the MBCA, the final raffinate stream or the extract stream comprising the diacid, thereby separating a C6- to C18-carbon diacid from the at least one impurity in the solution.2. A method for separating a C6- to C18-carbon diacid from at least one impurity in a solution , comprising:(a) introducing a feed stream comprising a solution comprising at least one 6- to 18-carbon ...

PROCESS TECHNOLOGY FOR BIOLOGICAL PRODUCT MANUFACTURING AND DOWNSTREAM PURIFICATION

Provided herein are, inter alia, biological manufacturing and downstream purification processes. 1. A process for purifying a biological product , comprising:receiving, via an input line, a heterogeneous mixture containing the biological product;removing impurities from the heterogeneous mixture by filtration in a dynamic filtration module by feeding the biological product from at least one output head in fluid communication with the input line to the dynamic filtration module under negative pressure, thereby producing a filtrate comprising the biological product, the dynamic filtration module comprising a dynamic filtration apparatus having a filter membrane extending between a feed reel and a collection reel with at least one support member having a substantially smooth contact surface, a target region of the filter membrane that is configured to receive the heterogeneous mixture from at least one output head, and a membrane support member with a substantially smooth contact surface that is in communication with a vacuum collection system that is positioned between the feed reel and the collection reel;transferring the filtrate to a first module capable of separating the solution into two or more fractions wherein at least one fraction contains the biological product, the first module comprising an affinity-based purification apparatus, wherein the first module has at least one first inlet and at least one first outlet configured to permit fluid flow between the at least one first inlet and the at least one first outlet via a mechanical rotary system comprising a vessel carousel containing at least one discrete vessel comprising a suspension of beads;transferring the fraction containing the biological product from the at least one outlet of the first module to a second module having at least one inlet for receiving flow from the at least one first outlet of the first module, the second module comprises at least one free-flow electrophoresis apparatus, wherein the ...

TWO-BED PARAFFIN TO OLEFIN ENHANCEMENT PROCESS

A process is presented for the purification of an olefins feed stream to a benzene alkylation unit. The process removes heavy aromatics in an adsorbent system comprising at least two adsorbent units. The unit passes the olefins feed stream to a first adsorbent unit, while the second adsorbent unit is either in regeneration mode, or standby mode. The process switches the feed stream to the second adsorbent unit and displaces the fluid in the second adsorbent unit, while maintaining the flow of the purified feed stream to the benzene alkylation unit. 1. A process for the removal of heavy aromatics from an olefins stream comprising:passing an olefins feed stream to a first adsorbent unit in an adsorbent system comprising at least two adsorbent units to generate a first adsorbent effluent stream with reduced heavy aromatics content;running the first adsorbent unit until breakthrough;equalizing pressure in a second adsorbent unit to the pressure of the first adsorbent unit;passing the olefins feed stream to the second adsorbent unit to generate a second adsorbent unit effluent stream with reduced heavy aromatics content;passing the second adsorbent unit effluent stream to the first adsorbent unit, and displacing the first adsorbent unit fluid;discontinuing the first adsorbent unit displacement;passing regenerant to the first adsorbent unit to regenerate the first adsorbent unit, wherein the regenerant flows in a counter-current direction relative to the olefin feed stream through the first adsorbent unit;equalizing the in the first adsorbent unit to the pressure of the second adsorbent unit;passing the olefins feed stream to the first adsorbent unit to generate the first adsorbent unit effluent stream;passing the first adsorbent unit effluent stream to the second adsorbent unit, wherein the first adsorbent unit effluent stream to the second adsorbent unit flows in a counter-current direction relative to the olefin feed stream through the second adsorbent unit, and ...

Expanded Bed Affinity Selection

Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

CONTINUOUS PROCESS FOR SEPARATION OF PROTEINS

Disclosed is a continuous process for separating or extracting proteins from a low grade mixture of a protein of interest, other proteins, impurities, and salts in a continuous simulated moving bed separation process. The invention provides for direct extraction of heme protein and plant protein from a crude mixture of such proteins, other proteins, impurities and salts using the chromatographic technique of simulated moving bed (SMB) continuous chromatography. The SMB process combines the steps of feed loading, adsorbent washing, product elution, adsorbent regeneration, and adsorbent equilibration. The novel strong anion exchange resin adsorbent is a quaternary amine cross-linked microcellulose wherein the microcellulose is cross-linked with epichlorohydrin and the quaternary amine is 2,3-epoxypropyltrimethyl-ammonium chloride which exhibits selective adsorption of proteins and complete regeneration. Purified protein separated in this manner may provide human health benefits by providing greater medicinal and nutrition opportunities from low quality protein sources. 1. A process for the continuous extraction of at least one protein of interest from a crude protein in a continuous simulated moving bed (SMB) extraction zone , said process comprising:a) admixing a crude protein comprising the at least one protein of interest, other proteins, and impurities with an equilibration buffer stream comprising a phosphate salt of sodium or potassium and water to provide an SMB feed stream: i) concurrently passing the SMB feed stream at SMB feed conditions to the top of the loading zone containing a first adsorbent bed to load the SMB feed mixture on the adsorbent in the loading zone and withdrawing a first waste stream comprising water and impurities from the bottom of the loading zone;', 'ii) concurrently passing the wash buffer stream comprising phosphate salt of sodium or potassium to the top of the washing zone to wash the adsorbent in the wash zone to provide a wash ...