Assay

Microfluidic arrangements

Microfluidic arrangements

DEVICE FOR MICRO TEST PROCEDURE

PROCEDURE FOR THE TRANSPORT OF FLUID SAMPLES BY A MICRO-FLUID CHANNEL

ELECTRICALKINETIC PIPETTE, AS WELL AS MEANS FOR COMPENSATING ELEKTROPHORETI DIVORCE EFFECTS

THERMAL MICRO VALVES

Electrowetting-driven micropumping

METHODS AND APPARATUS FOR MANIPULATING DROPLETS BY ELECTROWETTING-BASED TECHNIQUES

Method and apparatus for performing microassays

Device for optical and electrochemical measurements in microliter size samples

METHODS AND APPARATUS FOR FLOW-CONTROLLED WETTING

Methods of determining a first position at which a dispersed phase droplet wets a surface of a channel are provided herein. The methods include immersing the dispersed phase droplet in a continuous phase fluid, wherein the continuous phase fluid is immiscible with the dispersed phase droplet, subsequently flowing the dispersed phase droplet in the continuous phase through the channel at a dispersed phase droplet velocity, wherein the dispersed phase droplet is separated from the surface by a film of the continuous phase fluid having a film thickness, and reducing the film thickness to rupture the film at the first position, wherein the droplet wets the surface at the first position.

Multiple emulsions created using junctions

DEVICE OF PUMPING BY ELECTROMOUILLAGE AND APPLICATION TO MEASUREMENTS Of ELECTRIC ACTIVITY

L'invention concerne un procédé de pompage, à travers un orifice (30, 31) d'un premier substrat (1), d'un premier volume de liquide (2), en contact avec une première surface hydrophobe dudit substrat, dans lequel une variation de pression entre le premier volume de liquide et un deuxième volume de liquide (2'), situé en contact avec ledit orifice et une deuxième surface hydrophobe dudit substrat, est obtenue par électromouillage.

METHODS AND APPARATUS FOR MANIPULATING DROPLETS BY ELECTROWETTING-BASED TECHNIQUES

An apparatus is provided for manipulating droplets. The apparatus is a single-sided electrode design in which all conductive elements are contained on one surface on which droplets are manipulated. An additional surface can be provided parallel with the first surface for the purpose of containing the droplets to be manipulated. Droplets are manipulated by performing electrowetting-based techniques in which electrodes contained on or embedded in the first surface are sequentially energized and de-energized in a controlled manner. The apparatus enables a number of droplet manipulation processes, including merging and mixing two droplets together, splitting a droplet into two or more droplets, sampling a continuous liquid flow by forming from the flow individually controllable droplets, and iterative binary or digital mixing of droplets to obtain a desired mixing ratio. © KIPO & WIPO 2007 ...

MULTIPLE EMULSIONS CREATED USING JETTING AND OTHER TECHNIQUES

Virtual well plate system

A virtual well plate system includes a base including a base plate having an upper surface with a hydrophobic region which defines hydrophilic domains, each hydrophilic domain adapted to hold a droplet of liquid therein; a movable lid including a lid plate having a lower surface with a hydrophobic region which defines hydrophilic domains, each hydrophilic domain adapted to hold a liquid droplet in a hanging manner; and a resistance arrangement mounted to the base and/or lid and which maintains the base and lid in an assembled condition such that the base plate and lid plate are maintained at a sufficient distance to prevent formation of virtual wells by the droplets, and which permits movement of the lid toward the base upon application of an external force sufficient to overcome a resistance of the resistance arrangement, to form virtual wells by combining the droplets.

Control of emulsions, including multiple emulsions

The present invention generally relates to emulsions, and more particularly, to double and other multiple emulsions. Certain aspects of the present invention are generally directed to the creation of double emulsions and other multiple emulsions at a common junction of microfluidic channels. In some cases, the microfluidic channels at the common junction may have substantially the same hydrophobicity. In one set of embodiments, a device may include a common junction of six or more channels, where a first fluid flows through one channel, a second fluid flows through two channels, and a third or carrying fluid flows through two more channels, such that a double emulsion of a first droplet of the first fluid, contained in a second droplet of the second fluid, contained by the carrying fluid, flows away from the common junction through a sixth channel.

Method for analyzing plurality of liquid samples

Microfluidic arrangements

MICRO-FLUID DEVICE, MIXING PROCESS AND USE OF THE DEVICE

PROCEDURE AND DEVICE FOR THE MICRO TEST PROCEDURE

DEVICE TO THE SEQUENTIAL EXPENDITURE OF FLUID REAGENTS

Apparatus and methods for parallel processing of micro-volume liquid reactions

APPARATUS AND METHODS FOR PARALLEL PROCESSING OF MICRO-VOLUME LIQUID REACTIONS

Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

ASSAY DEVICE AND METHODS

The present invention relates to the use of amphipathic polymers to enhance lateral flow and reagent mixing on assay devices. More specifically, the invention relates to use of an amphipathic polymer in assay methods including a device for determining the concentration of lipids in blood serum or plasma.

METHOD AND APPARATUS FOR PERFORMING MICROASSAYS

A system and method for analyzing a plurality of liquid samples. The system has a platen having two substantially parallel planar surfaces and a plurality of through-holes dimensioned so as to maintain a liquid sample in each throughhole by means of surface tension. A source of optical radiation illuminates the through-holes, and an optical arrangement analyzes the light emanating from the through-holes. The through-holes may be individually addressable, and may have volumes less than 100 nanoliters. Samples may be drawn from a planar surface by capillary action and may be accurately dispensed, diluted and mixed in accordance with embodiments of the invention.

MOVING AND MIXING OF MICRODROPLETS THROUGH MICROCHANNELS

The movement and mixing of microdroplets through microchannels is described employing microscale device. Sample and reagent are injected into the device through entry ports (A) and they are transported as discrete droplets throug h channels (B) to a reaction chamber, such as a thermally controlled reactor where mixing and reactions (e.g., restriction enzyme digestion or nucleic ac id amplification) occur (C). The biochemical products are then moved by the sam e method to an electrophoresis module (D) where migration data is collected by a detector (E) and transmitted to a recording instrument. Importantly, the fluidic and electronic components are designed to be fully compatible in function and construction with the biological reactions and reagents. ...

THERMAL MICROVALVES

Thermal microvalves and their method of use include a fusible material (40) positioned in a microchannel (44). A heater (42) melts the material (40) which is then forced by air pressure (46) into a second channel (48) where it cools and solidifies, blocking flow. Alternatively, the melted and solidified material (40) can hold a diaphragm of a diaphragm valve in place, eliminating the need for continued applied force on the diaphragm.

Apparatus for preparing cosmetic composition containing emulsion substance formed by instant emulsification using microfluidic channel

An apparatus for preparing a cosmetic composition containing an emulsion substance formed by instant emulsification through a microfluidic channel is provided. The apparatus includes a housing defining the appearance of the apparatus and installed with a pump on one side, the pump being operated by a user; an external-phase chamber provided in the housing, and storing an external-phase fluid forming the external phase of the emulsion substance; a dispersed-phase chamber provided in the housing, and storing a dispersed-phase fluid forming the dispersed phase of the emulsion substance. The apparatus further includes a microfluidic channel providing a path for the external-phase fluid and the dispersed-phase fluid to flow for forming the emulsion substance by combining the external-phase fluid with the dispersed-phase fluid; and a tube for discharging the emulsion substance from the microfluidic channel.

MIXING OF FLUIDS IN FLUIDIC SYSTEMS

Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment (5) of a fluidic device. The fluids may be in the form of, for example, at least first (10-1), second (20-2) and third (10-3) fluid plugs, composed of first (10-1A), second (20-2A), and third (10-3A) fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids.

Thermal microvalves in a fluid flow method

The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, including microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

Apparatus and methods for parallel processing of micro-volume liquid reactions

Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

Apparatus and methods for parallel processing of micro-volume liquid reactions

Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

Apparatus for photometric analyses

DEVICE FOR PHOTOMETRIC ANALYSES.

Thermal microvalves

Assay device and methods

Methods and apparatus for manipulating droplets by electrowetting-based techniques

A microfluidic device and a method for provision of double emulsion droplets

A microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets.

A MICROFLUIDIC DEVICE AND A METHOD FOR PROVISION OF DOUBLE EMULSION DROPLETS

A microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets.

METHOD AND APPARATUS FOR PERFORMING MICROASSAYS

A system and method for analyzing a plurality of liquid samples. The system has a platen having two substantially parallel planar surfaces and a plurality of through-holes dimensioned so as to maintain a liquid sample in each through-hole by means of surface tension. A source of optical radiation illuminates the through-holes, and an optical arrangement analyzes the light emanating from the through-holes. The through-holes may be individually addressable, and may have volumes less than 100 nanoliters. Samples may be drawn from a planar surface by capillary action and may be accurately dispensed, diluted and mix ed in accordance with embodiments of the invention.

APPARATUS AND METHOD FOR MASS PRODUCING A MONODISPERSE MICROBUBBLE AGENT

An apparatus (1) for mass producing monodisperse microbubbles, comprising a microfluidic flow focusing device (2) including a dispersed phase fluid supply channel (110) having an outlet (114) that discharges into a flow focusing junction (140); a continuous phase fluid supply channel (120, 120') having an outlet (124) that discharges into the flow focusing junction; and a bubble formation channel (130) having an inlet (132) disposed at the flow focusing junction. The configuration of the flow focusing junction (140) is such that, in operation, a flow of dispersed phase fluid (12) discharging from the outlet (114) of the dispersed phase fluid supply channel (110) is engageable in co-flow by a focusing flow of continuous phase fluid discharging from the outlet of the at least one continuous phase fluid supply channel under formation of a gradually thinning jet of dispersed phase fluid that extends into the inlet of the bubble formation channel.

Multiple emulsions created using jetting and other techniques

The present invention generally relates to emulsions, and more particularly, to multiple emulsions. In one aspect, multiple emulsions are formed by urging a fluid into a channel, e.g., by causing the fluid to enter the channel as a “jet.” Multiple fluids may flow through a channel collinearly before multiple emulsion droplets are formed. The fluidic channels may also, in certain embodiments, include varying degrees of hydrophilicity or hydrophobicity. In some cases, the average cross-sectional dimension may change, e.g., at an intersection. Unexpectedly, systems such as those described herein may be used to encapsulate fluids in single or multiple emulsions that are difficult or impossible to encapsulate using other techniques, such as fluids with low surface tension, viscous fluids, or viscoelastic fluids. Other aspects of the invention are generally directed to methods of making and using such systems, kits involving such systems, emulsions created using such systems, or the like.

THREE-DIMENSIONAL MICROFLUIDIC SYSTEMS

A three-dimensional microfluidic system including: at least one hydrophilic thread along which fluid can be transported through capillary wicking; and at least one hydrophobic substrate for supporting the hydrophilic thread. A method of transporting and mixing a plurality of fluids within a microfluidic system including at least two hydrophilic threads and a hydrophobic substrate having at least two zones, each of the hydrophilic threads supported on a different hydrophobic substrate zone, including: delivering each said fluid to a different hydrophilic thread; and bringing the at least two hydrophilic threads into contact to cause mixing of the fluids.

High Resolution Temperature Profile Creation in a Digital Microfluidic Device

Designs of a digital microfluidic devices are described comprising droplet control electrodes and heating electrodes that have effects in the regions for droplet manipulations. Specifically, the digital microfluidic device comprises a first substrate having liquid control electrodes for droplet control and a second substrate having heating electrodes for temperature control. Shielding electrodes are disposed on the second substrate to ensure that the heating electrodes can control the digital microfluidic device to a desired temperature profile without interfering the droplet operations such as transport, merging/mixing, splitting, particle distribution, etc. 1. An apparatus for droplet manipulations , comprising:a. a first substrate comprising a first substrate surface;b. an array of droplet control electrodes disposed on the first substrate surface;c. a first dielectric layer disposed on the first substrate surface to cover at least some of the droplet control electrodes;d. a second substrate, comprising a second substrate surface facing the first substrate surface, spaced from the first substrate surface by a distance to define a space between the first and the second substrates, wherein the distance is sufficient to contain a droplet disposed in the space;e. one or more heating electrodes disposed on the second substrate surface;f. a second dielectric material layer disposed on the second substrate surface to cover at least some of the thin film heating electrode(s);g. one or more shielding electrodes disposed on the second substrate surface to cover at least a portion of the second dielectric material layer; andh. a third dielectric material layer disposed on the second substrate surface to cover at least some of the shielding electrodes.2. The apparatus of claim 1 , wherein two or more heating electrodes are connected in series.3. The apparatus of claim 1 , wherein two or more heating electrodes are connected in parallel.4. The apparatus of claim 1 , wherein ...

Methods for manipulating droplets by electrowetting-based techniques

Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

METHODS AND APPARATUS FOR MANIPULATING DROPLETS BY ELECTROWETTING-BASED TECHNIQUES

APPARATUS FOR PHOTOMETRIC ANALYSES

VERFAHREN UND VORRICHTUNG ZUR MIKROTESTDURCHFÜHRUNG

Microfluidic arrangements

A method of fabricating a microfluidic arrangement for manipulating fluids comprising arranging a first fluid on an unpatterned surface of a substrate in a desired shape, arranging a second fluid, which is immiscible with the first fluid, to cover the first fluid at least partially, wherein the first fluid is retained in said shape by a fluid interface between the first and second fluids and drying the first fluid to form a residue in said shape on the substrate. The arrangement maybe an array of drops and/or a circuit wherein droplets are connected by fluid conduits. Ideally, the first fluid is an aqueous liquid comprising cell culture medium, trehalose, buffered saline and sugar. The second fluid is a gas, oil or fluorocarbon. A microfluidic arrangement comprising a substrate with a surface and a residue on the surface wherein the residue is from a dried out fluid arranged in a desired shape. Introduction of a first fluid to the residue, rehydrates the residue such that the first fluid ...

ELECTRICALKINETIC PIPETTE, AS WELL AS MEANS FOR COMPENSATING ELEKTROPHORETI DIVORCE EFFECTS

ELECTRICAL WETTING PUMPING DEVICE AND YOUR USE FOR MEASURING ELECTRICAL ACTIVITY

Three-dimensional microfluidic systems

A three-dimensional microfluidic system including: at least one hydrophilic thread along which fluid can be transported through capillary wicking; and at least one hydrophobic substrate for supporting the hydrophilic thread. A method of transporting and mixing a plurality of fluids within a microfluidic system including at least two hydrophilic threads and a hydrophobic substrate having at least two zones, each of the hydrophilic threads supported on a different hydrophobic substrate zone, including: delivering each said fluid to a different hydrophilic thread; and bringing the at least two hydrophilic threads into contact to cause mixing of the fluids.

Electropipettor and compensation means for electrophoretic bias

APPARATUS AND METHODS FOR PARALLEL PROCESSING OF MICRO-VOLUME LIQUID REACTIONS

Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

METHODS FOR SCREENING SUBSTANCES IN A MICROWELL ARRAY

Methods for manufacturing and using an apparatus for manipulating and analyzing a large number of microscopic samples of a liquid or materials, including cells, in liquid suspension. Parallel through-holes are formed in a platen and loaded with a liquid. Loading may be performed in such a way as to create a gradient, with respect to the position of the through-holes, of the concentration of a particular substance or of another quantity. Mixing of the contents of through--holes may be obtained by bringing filled microwell arrays into contact with each other with registration of individuals through-holes.

METHODS FOR SCREENING SUBSTANCES IN A MICROWELL ARRAY

Methods for manufacturing and using an apparatus for manipulating and analyzing a large number of microscopic samples of a liquid or materials, including cells, in liquid suspension. Parallel through-holes (12) are formed in a platen (10) and loaded with a liquid. Loading may be performed in such a way as to create a gradient, with respect to the position of the through- holes, of the concentration of a particular substance or of another quantity. Mixing of the contents of through-holes may be obtained by bringing filled microwell arrays into contact with each other with registration of individuals through-holes.

THERMAL MICROVALVES

Thermal microvalves and their method of use include a fusible material (40) positioned in a microchannel (44). A heater (42) melts the material (40) which is then forced by air pressure (46) into a second channel (48) where it cools and solidifies, blocking flow. Alternatively, the melted and solidified material (40) can hold a diaphragm of a diaphragm valve in place, eliminating the need for continued applied force on the diaphragm.

Apparatus and methods for parallel processing of microvolume liquid reactions

Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

Electrowetting pumping device and application to electric activity measurements

A method for pumping through an orifice of a first substrate, a first volume of liquid in contact with a first hydrophobic surface of said substrate, wherein a pressure variation between the first volume of liquid and a second volume of liquid, located in contact with said orifice and a second hydrophobic surface of said substrate, is achieved by electrowetting.

ELEKTROBENETZUNGS-PUMPVORRICHTUNG UND IHRE VERWENDUNG ZUM MESSEN ELEKTRISCHER AKTIVITÄT

COMBINING CHEMICAL REAGENTS

Moving microdroplets

DEVICE FOR OPTICAL AND ELECTROCHEMICAL MEASUREMENTS IN MICROLITER SIZE SAMPLES

A small liquid sample (24) to be tested is contained within an annulus (18) on the upper surface (14) of a substrate (12). In one embodiment, a reagent diffuses into the sample through a membrane (28) in a junction hole (26). the junction hole connecting upper (14) and lower (16) surfaces of the substrates. Optical measuring equipment (70, 72) detects a measurable change in an optical property of the sample. In an alternate embodiment, a flow of gas (30) directed at a small liquid sample (24), causes the sample to flow in a controlled manner over the surface of an electrode (20), disposed on the substrate surface (14). In another alternate enbodiment, a spacial averaging electrode (20) is disposed in a non-homogeneous sample (24). Highly reproducible and accurate hydrodynamic electrochemical studies and analyses of microliter size samples are thus achievable, without the need for moving mechanical parts.

METHODS FOR SCREENING SUBSTANCES IN A MICROWELL ARRAY

Methods for manufacturing and using an apparatus for manipulating and analyzing a large number of microscopic samples of a liquid or materials, including cells, in liquid suspension. Parallel through-holes (12) are formed in a platen (10) and loaded with a liquid. Loading may be performed in such a way as to create a gradient, with respect to the position of the through- holes, of the concentration of a particular substance or of another quantity. Mixing of the contents of through-holes may be obtained by bringing filled microwell arrays into contact with each other with registration of individuals through-holes.

CHEMICAL DROPLET REACTOR

A method and apparatus for combining chemical reactants wherein the method comprises the steps of: 1) dispensing the reactants into a conduit system in the form of discrete volumes or droplets (5, 15, 21, 22, 36) separated from each other by an inert immiscible liquid (1); and 2) changing the flow of the inert immiscible liquid within the conduit system in a precise sequence in such a manner that predetermined discrete volumes coalesce with each other, thereby combining the reactants; and wherein the apparatus is formed by holding two plates (42, 44) together in face to face contact, where one or both plates possesses a set of indentations (43) on the surface to be mated, thereby forming the conduits for reactant combinations.

Mixing of fluids in fluidic systems

Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment of a fluidic device. The fluids may be in the form of, for example, at least first, second and third fluid plugs, composed of first, second, and third fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active to components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids.

VIRTUAL WELLS FOR USE IN HIGH THROUGHPUT SCREENING ASSAYS

Microtiter-like plates containing virtual wells formed by an arrangement of relatively hydrophilic domains within relatively hydrophobic fields are provided. Assay mixtures are confined to the hydrophilic domains of the virtual wells by the edges of the hydrophobic fields. The use of virtual wells allows one to perform homogeneous and capture and wash high throughput screening assays with assay mixtures having volumes on the order of about 100 nl to 10¿l. Virtual wells also provide a means of easily moving fluids, which is particularly useful for simultaneous additions needed for kinetic studies and flash detection and washing. Methods for controlling evaporation during the dispensing of reagents as well as during incubation of high throughput screening utilizing microtiter-like plates containing virtual wells are also provided. The present invention also provides an inexpensive, disposable device for transferring small volumes of an entire array of compounds from a first microtiter-like ...

Microfluidic arrangements

Microfluidic arrangements

MANIPULATION OF DROPLETS ON HYDROPHILIC OR VARIEGATED-HYDROPHILIC SURFACES

Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet- operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated- hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.

MULTIPLE EMULSIONS CREATED USING JUNCTIONS

The present invention generally relates to emulsions, and more particularly, to multiple emulsions. In one aspect, multiple emulsions are formed using a plurality of channels, such as microfluidic channels, that meet at a common intersection. The multiple emulsions may be created at a single common intersection in some embodiments, unlike other prior art systems where multiple channel intersections are required to create multiple emulsions. For instance, in one set of embodiments, three, four, or more microfluidic channels may intersect at a common intersection, with two or three serving as inlets and one serving as the outlet. In some embodiments, a first fluidic channel may be relatively hydrophobic, while a second fluidic channel is relatively hydrophilic. The third channel, if present, may be relatively hydrophilic or hydrophobic, depending on the application. The outlet channel may be hydrophobic, hydrophilic, or may comprise at least one portion that is relatively hydrophilic and ...

Virtual well plate system

A virtual well plate system includes a base including a base plate having an upper surface with a hydrophobic region which defines hydrophilic domains, each hydrophilic domain adapted to hold a droplet of liquid therein; a movable lid including a lid plate having a lower surface with a hydrophobic region which defines hydrophilic domains, each hydrophilic domain adapted to hold a liquid droplet in a hanging manner; and a resistance arrangement mounted to the base and/or lid and which maintains the base and lid in an assembled condition such that the base plate and lid plate are maintained at a sufficient distance to prevent formation of virtual wells by the droplets, and which permits movement of the lid toward the base upon application of an external force sufficient to overcome a resistance of the resistance arrangement, to form virtual wells by combining the droplets.

FLUIDIC CARTRIDGE FOR CYTOMETRY AND ADDITIONAL ANALYSIS

The disclosure relates to devices and methods for analyzing particles in a sample. In various embodiments, the present disclosure provides devices and methods for cytometry and additional analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and/or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing particles in a sample. 1. A device for analyzing target particles in a sample , comprising: an inlet configured for receiving the sample into the cartridge device;', 'a fluidic structure fluidly connected to the inlet and configured for mixing at least a portion of the sample with at least a portion of a reagent to form one or more sample mixtures;', 'a flow cell fluidly connected to the fluidic structure, wherein the flow cell is configured for forming one or more sample streams from the one or more sample mixtures and for measuring a signal from the one or more sample streams to detect target particles; and', 'a flow sensor fluidly connected to the flow cell and configured for measuring a sensing signal from the sample streams that enter the flow sensor; and, 'a cartridge device, wherein the cartridge device comprisesa reader instrument configured to measure the signal at the flow cell; the reagent comprises a fluorescent labeling agent that selectively labels the target particles in the sample with fluorescence;', 'a fluidic connection between the flow cell and the flow sensor is configured for forming a sample stream to have the same flow rate flowing through the flow cell and the flow sensor; and', 'the reader instrument is configured to measure the signal at the flow cell and the sensing signal from the flow sensor to quantify the target particles in the sample., 'wherein2. The device of claim 1 , wherein the flow cell is configured to form the one or ...

THERMAL MICROVALVES

Thermal microvalves and their method of use include a fusible material (40) positioned in a microchannel (44). A heater (42) melts the material (40) which is then forced by air pressure (46) into a second channel (48) where it cools and solidifies, blocking flow. Alternatively, the melted and solidified material (40) can hold a diaphragm of a diaphragm valve in place, eliminating the need for continued applied force on the diaphragm.

Microfluidic arrangements

Device for optical and electrochemical measurements in microliter size samples

Multi-manifold device for carrying out chemical, biological and/or biochemical analytical methods

Methods and apparatus for manipulating droplets by electrowetting-based techniques

Titration method

DEVICE FOR OPTICAL AND ELECTROCHEMICAL MEASUREMENTS IN MICROLITER SIZE SAMPLES

A small liquid sample (24) to be tested is contained within an annulus (18) on the upper surface (14) of a substrate (12). In one embodiment, a reagent diffuses into the sample through a membrane (28) in a junction hole (26), the junction hole connecting upper (14) and lower (16) surfaces of the substrates. Optical measuring equipment (70, 72) detects a measurable change in an optical property of the sample. In an alternate embodiment, a flow of gas (30) directed at a small liquid sample (24), causes the sample to flow in a controlled manner over the surface of an electrode (20), disposed on the substrate surface (14). In another alternate enbodiment, a spacial averaging electrode (20) is disposed in a non-homogeneous sample (24). Highly reproducible and accurate hydrodynamic electrochemical studies and analyses of microliter size samples are thus achievable, without the need for moving mechanical parts.

MIXING OF FLUIDS IN FLUIDIC SYSTEMS

Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment (5) of a fluidic device. The fluids may be in the form of, for example, at least first (10-1), second (20-2) and third (10-3) fluid plugs, composed of first (10-1A), second (20-2A), and third (10-3A) fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids.

FLUIDIC MIXING STRUCTURE, METHOD FOR FABRICATING SAME, AND MIXING METHOD

A fluidic micromixer compπses a plurality of fluid inlets in communication with a mixing chamber, the plurality of fluid inlets being adapted to introduce a plurality of distinct fluid streams into the chamber The mixing chamber comprises at least one surface patterned to define hydrophobic and hydrophilic regions spaced apart along a pπncipal direction of fluid flow within the chamber from the fluid inlets to a fluid outlet, the regions being adapted to induce fluid flow in a direction transverse to the pnncipal direction of fluid flow to mix the fluid streams At least one of the hydrophobic regions may compnse microstructures patterned on the at least one surface.

TITRATION METHOD

The invention relates to a titration method for small quantities of liquid. According the invention, a drop of an analyte which is maintained by means of the surface tension thereof is disposed on the essentially level surface of a solid body, preferably a solid body chip. An amount of titrant is brought into contact with the analyte drop in order to create a reaction, said amount being smaller than the amount of the analyte drop, and a characteristic variable for the reaction between the titrant and the analyte is measured during and/or after the reaction.

Mixing of fluids in fluidic systems

Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment of a fluidic device. The fluids may be in the form of, for example, at least first, second and third fluid plugs, composed of first, second, and third fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids.

Control of emulsions, including multiple emulsions

The present invention generally relates to emulsions, and more particularly, to double and other multiple emulsions. Certain aspects of the present invention are generally directed to the creation of double emulsions and other multiple emulsions at a common junction of microfluidic channels. In some cases, the microfluidic channels at the common junction may have substantially the same hydrophobicity. In one set of embodiments, a device may include a common junction of six or more channels, where a first fluid flows through one channel, a second fluid flows through two channels, and a third or carrying fluid flows through two more channels, such that a double emulsion of a first droplet of the first fluid, contained in a second droplet of the second fluid, contained by the carrying fluid, flows away from the common junction through a sixth channel. Other aspects of the invention are generally directed to methods of making and using such systems, kits involving such systems, emulsions created using such systems, or the like.

METHODS AND APPARATUS FOR MANIPULATING DROPLETS BY ELECTROWETTING-BASED TECHNIQUES

An apparatus is provided for manipulating droplets. The apparatus is a single-sided electrode design in which all conductive elements are contained on one surface on which droplets are manipulated. An additional surface can be provided parallel with the first surface for the purpose of containing the droplets to be manipulated. Droplets are manipulated by performing electrowetting-based techniques in which electrodes contained on or embedded in the first surface are sequentially energized and de-energized in a controlled manner. The apparatus enables a number of droplet manipulation processes, including merging and mixing two droplets together, splitting a droplet into two or more droplets, sampling a continuous liquid flow by forming from the flow individually controllable droplets, and iterative binary or digital mixing of droplets to obtain a desired mixing ratio.

Elektrokinetische Pipette, sowie Mittel zum Kompensieren von elektrophoretischen Scheidungseffekten

Combining chemical reagents

The invention is a method of combining chemical reagents and comprises the steps of: 1) Dispensing the reagents into a conduit system in the form of discrete volumes or droplets separated from each other by an inert immiscible liquid, and 2) Moving the inert liquid and discrete droplets in such a manner that predetermined discrete volumes coalesce with each other, thereby combining the reagents. The preferred apparatus for carrying out the method comprises a plastic block (2) with indentations (3) which is clamped against a glass plate (4) forming closed conduits which are connected to reservoirs of light mineral oil, or silicon oil, by thin tubing (9) and valves (10). All the conduits are initially filled with the oil, then the second and fourth tubes are used to introduce two reagents, which are separated into two smaller volumes at the 'T' junctions then these smaller volumes are merged at the 'Y' junction by withdrawing oil from the center conduit. ...

Microfluidic arrangements

A method of rehydrating a microfluidic arrangement wherein when hydrated the microfluidic arrangement comprises a first liquid on a surface of a substrate in a desired shape and a second liquid which is immiscible with the first liquid covering the first liquid, wherein the first liquid is retained in the shape by a liquid interface between the first and second liquids, the method comprising providing a dried out residue in the shape on the substrate, overlaying the residue with the second liquid and rehydrating the residue with the first liquid by passing the first liquid through the second liquid to form the microfluidic arrangement. The shape consists of an array of drops in a regularly spaced arrangement with a contact angle between each drop and the surface of the substrate being less than 50% of the advancing contact angle, or at least one liquid conduit in liquid communication with at least one liquid drop. The arrangement may be used for processing fluids or subjecting a fluid to ...

Moving microdroplets

Device for sequential discharge of flowable reagents

CHEMICAL DROPLET REACTOR

THERMAL MICROVALVES

Thermal microvalves and their method of use include a fusible material (40) positioned in a microchannel (44). A heater (42) melts the material (40) which is then forced by air pressure (46) into a second channel (48) where it cools and solidifies, blocking flow. Alternatively, the melted and solidified material (40) can hold a diaphragm of a diaphragm valve in place, eliminating the need for continued applied force on the diaphragm.

DEVICE OF PUMPING BY ELECTROMOUILLAGE AND APPLICATION TO MEASUREMENTS Of ELECTRIC ACTIVITY

Microscale devices and reactions in microscale devices

The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

Electropipettor and compensation means for electrophoretic bias

The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along ...

Methods for performing microfluidic sampling

Methods for sampling a liquid flow are provided. The method includes supplying a continuous liquid flow from a continuous flow fluid input source to a surface along a first flow path. The method additionally includes sampling the continuous liquid flow by performing an electric field-based technique to split off a sample droplet from a portion of the liquid flow, whereby the sample droplet is distinct from the liquid flow and controllable independently of the liquid flow, wherein the electric field-based technique is performed by providing a set of electrodes on the surface and selectively biasing the set of electrodes, whereby the sample droplet is formed on one of the set of electrodes.

Assay device and methods

The present invention relates to the use of amphipathic polymers to enhance lateral flow and reagent mixing on assay devices. More specifically, the invention relates to use of an amphipathic polymer in assay methods including a device for determining the concentration of lipids in blood serum or plasma.

APPARATUS FOR MASS PRODUCING A MONODISPERSE MICROBUBBLE AGENT

An apparatus for mass producing monodisperse microbubbles includes a microfluidic flow focusing device, which includes a dispersed phase fluid supply channel having an outlet that discharges into a flow focusing junction, a continuous phase fluid supply channel having an outlet that discharges into the flow focusing junction, and a bubble formation channel having an inlet disposed at the flow focusing junction. The configuration of the flow focusing junction is such that, in operation, a flow of dispersed phase fluid discharging from the outlet of the dispersed phase fluid supply channel is engageable in co-flow by a focusing flow of continuous phase fluid discharging from the outlet of the at least one continuous phase fluid supply channel under formation of a gradually thinning jet of dispersed phase fluid that extends into the inlet of the bubble formation channel. 1. An apparatus for mass producing monodisperse microbubbles , comprising: a dispersed phase fluid supply channel having an outlet that discharges into a flow focusing junction;', 'at least one continuous phase fluid supply channel having an outlet that discharges into the flow focusing junction; and', 'a bubble formation channel having an inlet disposed at the flow focusing junction,, 'at least one microfluidic flow focusing device including 'wherein said bubble formation channel has a length that is much greater than its hydraulic diameter by a factor of at least ten.', 'the configuration of the flow focusing junction being such that, in operation, a flow of dispersed phase fluid discharging from the outlet of the dispersed phase fluid supply channel is engageable in co-flow by a focusing flow of continuous phase fluid discharging from the outlet of the at least one continuous phase fluid supply channel under formation of a gradually thinning jet of dispersed phase fluid that extends into the inlet of the bubble formation channel,'}2. The apparatus according to claim 1 , further comprising:a source ...

MULTIPLE EMULSIONS CREATED USING JETTING AND OTHER TECHNIQUES

The present invention generally relates to emulsions, and more particularly, to multiple emulsions. In one aspect, multiple emulsions are formed by urging a fluid into a channel, e.g., by causing the fluid to enter the channel as a “jet.” Side channels can be used to encapsulate the fluid with a surrounding fluid. In some cases, multiple fluids may flow through a channel collinearly before multiple emulsion droplets are formed. The fluidic channels may also, in certain embodiments, include varying degrees of hydrophilicity or hydrophobicity. As examples, the fluidic channel may be relatively hydrophilic upstream of an intersection (or other region within the channel) and relatively hydrophobic downstream of the intersection, or vice versa. In some cases, the average cross-sectional dimension may change, e.g., at an intersection. For instance, the average cross-sectional dimension may increase at the intersection. Surprisingly, a relatively small increase in dimension, in combination with a change in hydrophilicity of the fluidic channel, may delay droplet formation of a stream of collinearly-flowing multiple fluids under certain flow conditions; accordingly, the point at which multiple emulsion droplets are formed can be readily controlled within the fluidic channel. In some cases, the multiple droplet may be formed from the collinear flow of fluids at (or near) a single location within the fluidic channel. In addition, unexpectedly, systems such as those described herein may be used to encapsulate fluids in single or multiple emulsions that are difficult or impossible to encapsulate using other techniques, such as fluids with low surface tension, viscous fluids, or viscoelastic fluids. Other aspects of the invention are generally directed to methods of making and using such systems, kits involving such systems, emulsions created using such systems, or the like. 160-. (canceled)61. A method , comprising:providing a first aqueous fluid in a main microfluidic channel; ...

APPARATUS AND METHOD FOR MASS PRODUCING A MONODISPERSE MICROBUBBLE AGENT

An apparatus for mass producing monodisperse microbubbles contains a microfluidic flow focusing device. The microfluidic flow focusing device includes a dispersed phase fluid supply channel having an outlet that discharges into a flow focusing junction, a continuous phase fluid supply channel having an outlet that discharges into the flow focusing junction, and a bubble formation channel having an inlet disposed at the flow focusing junction. The configuration of the flow focusing junction is such that, in operation, a flow of dispersed phase fluid discharging from the outlet of the dispersed phase fluid supply channel is engageable in co-flow by a focusing flow of continuous phase fluid discharging from the outlet of the at least one continuous phase fluid supply channel under formation of a gradually thinning jet of dispersed phase fluid that extends into the inlet of the bubble formation channel. 1. An apparatus for mass producing monodisperse microbubbles , comprising: a dispersed phase fluid supply channel having an outlet that discharges into a flow focusing junction;', 'at least one continuous phase fluid supply channel having an outlet that discharges into the flow focusing junction; and', 'a bubble formation channel having an inlet disposed at the flow focusing junction,, 'at least one microfluidic flow focusing device comprisinga configuration of the flow focusing junction being such that, in operation, a flow of dispersed phase fluid discharging from the outlet of the dispersed phase fluid supply channel is engageable in co-flow by a focusing flow of continuous phase fluid discharging from the outlet of the at least one continuous phase fluid supply channel under formation of a gradually thinning jet of dispersed phase fluid that extends into the inlet of the bubble formation channel,{'sub': bfc', 'bfc', 'bfc', 'bfc, 'wherein said bubble formation channel has a length Land a hydraulic diameter D, and wherein L/D>>1.'}2. The apparatus according to claim 1 ...

MIXING OF FLUIDS IN FLUIDIC SYSTEMS

Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment of a fluidic device. The fluids may be in the form of, for example, at least first, second and third fluid plugs, composed of first, second, and third fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids. 1flowing in a channel a series of fluid plugs comprising a first fluid plug comprising a first fluid, a second fluid plug comprising a second fluid, and a third fluid plug comprising a third fluid, wherein the first fluid plug has a first volume,wherein the second fluid plug is positioned between the first and third fluid plugs, andwherein the second fluid is immiscible with each of the first and third fluids;reducing the first volume of the first fluid plug by at least 50%; andcombining at least a portion of the first fluid into the third fluid plug so as to mix at least portions of the first and third fluids.. A method, comprising: The present application is a continuation of U.S. patent application Ser. No. 14/175,044, filed Feb. 7, 2014, and entitled “Mixing of Fluids in Fluidic Systems,” which claims priority under 35 U.S.C. §119(e) to U.S. Provisional Patent Application No. 61/778,905, filed Mar. 13, 2013, and entitled “Mixing of Fluids in Fluidic Systems,” each of which is incorporated herein by reference in its entirety.The present embodiments relate generally to methods for flowing fluids in fluidic devices, and more specifically, to methods ...

MAGNETIC DIGITAL MICROFLUIDIC APPARATUS AND METHOD OF MAGNETIC DIGITAL MICROFLUIDIC MANIPULATION

A magnetic digital microfluidic apparatus for manipulating a liquid droplet containing magnetic particles using a magnetic force, the apparatus comprising: a hydrophobic surface on which the liquid droplet containing magnetic particles can be moved using the magnetic force; and at least one surface energy trap provided to retain at least a portion of the liquid droplet thereon, the at least one surface energy trap comprising a layer of polydopamine. A method of magnetic digital microfluidic manipulation, the method comprising the steps of: 1. A magnetic digital microfluidic apparatus for manipulating a liquid droplet containing magnetic particles using a magnetic force , the apparatus comprising:a hydrophobic surface on which the liquid droplet containing magnetic particles can be moved using the magnetic force; andat least one surface energy trap provided to retain at least a portion of the liquid droplet thereon, the at least one surface energy trap comprising a layer of polydopamine.2. The apparatus of claim 1 , wherein in use claim 1 , the at least one surface energy trap retains all of the liquid droplet thereon while allowing the magnetic particles to be moved out of the liquid droplet by the magnetic force.3. The apparatus of claim 1 , wherein in use claim 1 , the at least one surface energy trap retains only a part of the liquid droplet thereon while allowing a remainder of the liquid droplet containing the magnetic particles to be moved away from the droplet manipulator by the magnetic force.4. The apparatus of claim 1 , wherein the at least one surface energy trap is formed on the hydrophobic surface on which the liquid droplet containing magnetic particles can be moved.5. The apparatus of claim 1 , further comprising at least one droplet manipulator provided on a platform claim 1 , wherein the at least one hydrophilic surface energy trap is formed on the at least one droplet manipulator.6. The apparatus of claim 5 , wherein the at least one droplet ...

EWOD SYSTEM AND METHODS TO INCREASE DYNAMIC RANGE FOR DIGITAL NUCLEIC ACID AMPLIFICATION

A method of performing a digital quantification of a species in an electrowetting on dielectric (EWOD) device comprising the steps of: inputting a sample volume into the EWOD device; inputting a diluent volume into the EWOD device; performing an electrowetting operation to generate a first sample droplet from the sample volume; performing an amplification process on the first sample droplet within the EWOD device; measuring a turn-on value for the sample droplet; comparing the measured turn-on value of the sample droplet to a target turn-on value for digital quantification; calculating a dilution factor based on the comparison of the measured turn-on value of the sample droplet to the target turn-on value; performing an electrowetting operation to extract a second sample droplet from the sample volume; performing an electrowetting operation to dilute the second sample droplet with the diluent volume in accordance with the dilution factor to form a diluted second sample droplet; and performing a digital quantification on the diluted second sample droplet to quantify an initial concentration of the species in the sample volume. 1. A method of performing a digital quantification of a species in an electrowetting on dielectric (EWOD) device comprising the steps of:inputting a sample volume into the EWOD device;inputting a diluent volume into the EWOD device;performing an electrowetting operation to generate a first sample droplet from the sample volume;performing an amplification process on the first sample droplet within the EWOD device;measuring a turn-on value for the sample droplet;comparing the measured turn-on value of the sample droplet to a target turn-on value for digital quantification;calculating a dilution factor based on the comparison of the measured turn-on value of the sample droplet to the target turn-on value;performing an electrowetting operation to extract a second sample droplet from the sample volume;performing an electrowetting operation to dilute ...

Adpative droplet operations in an am-ewod device based on test measurement of droplet properties

A control method and related apparatus are disclosed for controlling actuation voltages applied to array elements of an element array on an electrowetting on dielectric (EWOD) device, wherein test metrics are determined and employed for optimizing subsequent droplet manipulation operations. The control method includes the steps of: receiving a liquid droplet onto the element array; applying an electrowetting actuation pattern of actuation voltages to actuate the droplet to modify a footprint of the droplet from a first state having an initial footprint to a second state having a modified footprint; sensing the modified footprint with a sensor; determining a test metric from sensing the modified footprint indicative of one or more droplet properties based on a droplet response of the liquid droplet to the electrowetting actuation pattern; and controlling actuation voltages applied to the array elements based on the test metric. The test metrics may include a transition rate and/or conformance to an actuation pattern.

Fluidic cartridge for cytometry and additional analysis

The disclosure relates to devices and methods for analyzing particles in a sample. In various embodiments, the present disclosure provides devices and methods for cytometry and additional analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and/or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing particles in a sample.

Apparatus and methods for parallel processing of micro-volume liquid reactions

Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

Apparatus and methods for parallel processing of microvolume liquid reactions

Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

Apparatus and methods for parallel processing of micro-volume liquid reactions

Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

Assay test card

The present invention relates to the use of surfaces that exhibit different surface energies wherein the difference in surface energies is configured to disrupt capillary laminar flow of a fluid travelling between the two surfaces. The invention further relates to the use of such surfaces in assay methods including a device utilising same.

Apparatus and method for mass producing a monodisperse microbubble agent

An apparatus for mass producing monodisperse microbubbles contains a microfluidic flow focusing device. The microfluidic flow focusing device includes a dispersed phase fluid supply channel having an outlet that discharges into a flow focusing junction, a continuous phase fluid supply channel having an outlet that discharges into the flow focusing junction, and a bubble formation channel having an inlet disposed at the flow focusing junction. The configuration of the flow focusing junction is such that, in operation, a flow of dispersed phase fluid discharging from the outlet of the dispersed phase fluid supply channel is engageable in co-flow by a focusing flow of continuous phase fluid discharging from the outlet of the at least one continuous phase fluid supply channel under formation of a gradually thinning jet of dispersed phase fluid that extends into the inlet of the bubble formation channel.