Fluid injection

The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling ...

Fluid injection

The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling ...

FLUID INJECTION

The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field. 135-. (canceled)36. A method , comprising:providing a microfluidic system comprising a first microfluidic channel and a second microfluidic channel contacting the first microfluidic channel at an intersection, wherein the microfluidic system further comprises first and second electrodes positioned to create an electric field within the intersection;providing a first fluidic droplet in the first microfluidic channel and a second fluid in the second microfluidic channel, wherein the first fluidic droplet and the second fluid contact each other forming a fluidic interface at least partially within the intersection andflowing a predetermined volume of the second fluid into the first fluidic droplet by applying a voltage to the first and second electrodes, wherein the volume of the second fluid flowing into the first fluidic droplet is partially controlled by controlling the voltage applied to the first and second electrodes.37. The method of claim 36 , further ...

Thermal Microvalves

The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

Apparatus and Process for Producing Patterned, Micron and Nanometer Size Reaction and Mixing Zones for Fluids Deposited on Smooth, Rough and Porous Surfaces and Applications of that Process

Process for producing patterned, micron and nanometer scale features by reacting or mixing in the small volumes at the intersections of coalescing drops, at the fronts of colliding thin films in front of spreading drops, or in the pore space of a porous medium under the drop. The process can be implemented on smooth, rough or porous surfaces and embodiments include multiplexed, single drop chemical or biochemical sensors and encryption of information of a printed page.

Stabilized droplets for calibration and testing

Provided herein, are droplet mixture compositions and systems and methods for forming mixtures of droplets. The system may comprise two or more droplet generation units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The combined droplet populations can be mixed, heated, and collected for multiple uses, such as for use as calibration standards for instrument testing and analysis.

Droplet-based analysis method

Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.

Thermal microvalves in a fluid flow method

The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, including microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

Microanalysis apparatus with constant pressure pump system

Micro-analysis system for analysing species with a fluid medium, said system containing sensing means for collecting species from the medium, said sensing means having an inlet and an outlet, analysing means comprising a channel, said channel defining at least one part for mixing, at least one part for reacting and at least one part for measuring detecting arrangement for determining the concentration of species at said part for measuring, a first fluid reservoir holding carrier fluid and at least one second fluid reservoir holding reagent fluid, connecting means, comprising first connecting means for fluid connection between the first fluid reservoir and the analysing means, second connecting means for fluid connection between the second fluid reservoir and the inlet of the sensing means and third connecting means for fluid connection between the outlet of the sensing means and the analysing means, said first connecting means comprising at least one first flow restricting means and said second connecting means comprising at least one second flow restricting means characterised by said micro-analysis system further comprising storage means containing said first fluid reservoir and said second fluid reservoir, said storage means being in downstream flow connection with means for pressurizing said fluid reservoirs, said storage means and said pressurizing means being separated from said analysing means.

Droplet generator based on high aspect ratio induced droplet self-breakup

A droplet generator apparatus and droplet generation method based on high aspect ratio induced droplet self-breakup are provided. The droplet generator apparatus includes a channel (1) and a nozzle (2) connected to the channel(1), and the aspect ratio of the channel (1) can be 3.0 or greater. The apparatus may further include a blocking rail (10) that is positioned in front of the nozzle (2), a supplying rail(9) that is positioned in front of the nozzle (2), and a supplying trench (8) formed in a space between the nozzle (2) and the supplying rail (9).

Integrated active flux microfluidic devices and methods

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

Integrated active flux microfluidic devices and methods

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

Integrated active flux microfluidic devices and methods

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

Integrated active flux microfluidic devices and methods

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

Integrated active flux microfluidic devices and methods

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e. Polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene.

Droplet-based analysis method

Droplet-based methods of analysis. In an exemplary method, a device having a port connected to a chamber may be selected. A sample-containing fluid may be placed into the port. A pressure differential may be created that drives the sample-containing fluid from the port to the chamber and separates the sample-containing fluid into droplets. A two-dimensional monolayer of the droplets may be formed in the chamber. At least a portion of the monolayer may be imaged.

Stabilized droplets for calibration and testing

Provided herein, are droplet mixture compositions and systems and methods for forming mixtures of droplets. The system may comprise two or more droplet generation units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The combined droplet populations can be mixed, heated, and collected for multiple uses, such as for use as calibration standards for instrument testing and analysis.

Capillary reactor distribution device and method

A capillary reactor distribution device comprising first and second capillary pathways ( 2, 3 ) which meet at a junction ( 5 ) and a third capillary pathway ( 4 ) which leads away from the junction ( 5 ), the capillary pathways ( 2, 3, 4 ) being dimensioned such that, when first and second immiscible fluids ( 14, 15 ) are fed along respectively the first and second capillary pathways ( 2, 3 ) under predetermined laminar flow conditions, the first and second fluids ( 14, 15 ) chop each other into discrete slugs ( 16, 17 ) which pass along the third capillary pathway ( 4 ). Molecular mixing between the fluids ( 14, 15 ) takes place by way of axial diffusion between adjacent slugs ( 16, 17 ) and by way of internal circulation within each slug ( 16, 17 ) as the slugs ( 16, 17 ) progress along the third capillary pathway ( 4 ).

Integrated active flux microfluidic devices and methods

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

Fluid injection

The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.

Integrated active flux microfluidic devices and methods

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

Fluid injection

The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.

Systems for encapsulation of actives within droplets and other compartments

The present invention generally relates to microparticles and, in particular, to systems and methods for encapsulation within microparticles. In one aspect, the present invention is generally directed to microparticles containing entities therein, where the entities contain an agent that can be released from the microparticles, e.g., via diffusion. In some cases, the agent may be released from the microparticles without disruption of the microparticles. The entities may be, for instance, polymeric particles, hydrogel particles, droplets of fluid, etc. The entities may be contained within a fluid that is, in turn, encapsulated within the microparticle. The agent may be released from the entity into the fluid, and then from the fluid through the microparticle. In such fashion, the release of agent from the microparticle may be controlled, e.g., over relatively long time scales. Other embodiments of the present invention are generally directed to methods of making such microparticles, methods of using such microparticles, microfluidic devices for making such microparticles, and the like.

Compositions comprising encapsulated actives within droplets and other compartments

The present invention generally relates to microparticles and, in particular, to systems and methods for encapsulation within microparticles. In one aspect, the present invention is generally directed to microparticles containing entities therein, where the entities contain an agent that can be released from the microparticles, e.g., via diffusion. In some cases, the agent may be released from the microparticles without disruption of the microparticles. The entities may be, for instance, polymeric particles, hydrogel particles, droplets of fluid, etc. The entities may be contained within a fluid that is, in turn, encapsulated within the microparticle. The agent may be released from the entity into the fluid, and then from the fluid through the microparticle. In such fashion, the release of agent from the microparticle may be controlled, e.g., over relatively long time scales. Other embodiments of the present invention are generally directed to methods of making such microparticles, methods of using such microparticles, microfluidic devices for making such microparticles, and the like.

Patent RU2017114553A3

РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2017 114 553 A (51) МПК A61K 9/50 (2006.01) A61K 8/11 (2006.01) A61Q 13/00 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2017114553, 19.11.2015 (71) Заявитель(и): ДЗЕ ПРОКТЕР ЭНД ГЭМБЛ КОМПАНИ (US), ПРЕЗИДЕНТ ЭНД ФЕЛЛОУС ОФ ХАРВАРД КОЛЛЕДЖ (US) Приоритет(ы): (30) Конвенционный приоритет: 24.11.2014 US 62/083,729 36 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 26.06.2017 US 2015/061482 (19.11.2015) (87) Публикация заявки PCT: WO 2016/085740 (02.06.2016) R U (54) Композиции, содержащие активные агенты, инкапсулированные в капли и другие полости (57) Формула изобретения 1. Композиция, характеризующаяся тем, что содержит множество микрочастиц, при этом каждая микрочастица содержит одну сердцевину, содержащую жидкую эмульсию, содержащую непрерывную фазу и диспергированную фазу; и оболочку, окружающую одну сердцевину, причем оболочка содержит стенку, имеющую среднюю толщину стенки от приблизительно 0,1 мкм до приблизительно 10 мкм. 2. Композиция по п. 1, в которой непрерывная фаза микрокапсул содержит гидрогель. 3. Композиция по п. 2, в которой доля удержания диспергированной фазы составляет более чем приблизительно 40%. 4. Композиция по п. 1, в которой диспергированная фаза содержит вещество, выбранное из группы, состоящей из проп-2-енил 3-циклогексилпропаноата, (4aR,5R,7aS,9R)-октагидро-2,2,5,8,8,9а-гексаметил-4h-4а,9-метаноазулено(5,6-d)-1,3диоксола, (3aR,5aS,9aS,9bR)-3а,6,6,9а-тетраметил-2,4,5,5а,7,8,9,9b-октагидро-1Н-бензо [е][1]бензофурана, 4-метоксибензальдегида, бензил-2-гидроксибензоата, 2метоксинафталина, 3-(4-трет-бутилфенил)пропаналя, 3а,6,6,9а-тетраметил2,4,5,5а,7,8,9,9b-октагидро-1Н-бензо[е][1]бензофурана, 3,7-диметилокт-6-ен-1-ола, 3,7диметилокт-6-еннитрила, 3-(4-трет-бутилфенил)бутаналя, 3-(4-пропан-2-илфенил) бутаналя, (Е)-1-(2,6,6-триметил-1-циклогекса-1,3-диенил)бут-2-ен-1-она, деканаля, (Е) -1-(2,6,6-триметил-1-циклогекс-3-енил)бут-2- ...

Crystal forming apparatus and method for using same

A crystal forming apparatus and method for using the apparatus, the method including depositing a precipitant solution in a site, incubating the site, during which time volatile vapor evaporates from the precipitant solution and accumulates in the site, and pumping the accumulated volatile vapor away from the site. An exemplary apparatus includes a sealed site except for a vent on the sealed site. In one embodiment, the vent is a passive vent that inhibits vapor diffusion out of the site. In another embodiment, the vent is an active vent that opens in response to a pressure differential. The present invention accelerates and controls the crystal growth process by pumping volatile vapor away from the sealed site.

For generating the micro-fluidic chip of spherical microbubble

本发明公开了一种用于生成球状微气泡的微流控芯片。本发明由两片内微通道结构不同的芯片键合而成，两片芯片的键合面均采用光刻蚀技术刻蚀出一定厚度与宽度的微通道。其中第一芯片内有两条液体微通道，一条气体微通道、微气泡生成通道和微气泡输出通道，两条液体微通道和气体微通道交界处存在一定角度的夹角；第二芯片内有与第一芯片结构尺寸相同的液体微通道、气体微通道和微气泡输出通道，但没有微气泡生成微通道。两片芯片键合而成后，同时向微通道内注入液体和气体，通过调节液体流量，控制气体压强，生成不同直径大小的微气泡。本发明突破了微流元件生成的微气泡在结构形状方面的局限性。

Systems for encapsulation of actives within droplets and other compartments

The present invention generally relates to microparticles and, in particular, to systems and methods for encapsulation within microparticles. In one aspect, the present invention is generally directed to microparticles containing entities therein, where the entities contain an agent that can be released from the microparticles, e.g., via diffusion. In some cases, the agent may be released from the microparticles without disruption of the microparticles. The entities may be, for instance, polymeric particles, hydrogel particles, droplets of fluid, etc. The entities may be contained within a fluid that is, in turn, encapsulated within the microparticle. The agent may be released from the entity into the fluid, and then from the fluid through the microparticle. In such fashion, the release of agent from the microparticle may be controlled, e.g., over relatively long time scales. Other embodiments of the present invention are generally directed to methods of making such microparticles, methods of using such microparticles, microfluidic devices for making such microparticles, and the like.

Compositions containing active agents encapsulated in drops and other cavities

РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2017 114 553 A (51) МПК A61K 9/50 (2006.01) A61K 8/11 (2006.01) A61Q 13/00 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2017114553, 19.11.2015 (71) Заявитель(и): ДЗЕ ПРОКТЕР ЭНД ГЭМБЛ КОМПАНИ (US), ПРЕЗИДЕНТ ЭНД ФЕЛЛОУС ОФ ХАРВАРД КОЛЛЕДЖ (US) Приоритет(ы): (30) Конвенционный приоритет: 24.11.2014 US 62/083,729 36 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 26.06.2017 US 2015/061482 (19.11.2015) (87) Публикация заявки PCT: WO 2016/085740 (02.06.2016) R U (54) Композиции, содержащие активные агенты, инкапсулированные в капли и другие полости (57) Формула изобретения 1. Композиция, характеризующаяся тем, что содержит множество микрочастиц, при этом каждая микрочастица содержит одну сердцевину, содержащую жидкую эмульсию, содержащую непрерывную фазу и диспергированную фазу; и оболочку, окружающую одну сердцевину, причем оболочка содержит стенку, имеющую среднюю толщину стенки от приблизительно 0,1 мкм до приблизительно 10 мкм. 2. Композиция по п. 1, в которой непрерывная фаза микрокапсул содержит гидрогель. 3. Композиция по п. 2, в которой доля удержания диспергированной фазы составляет более чем приблизительно 40%. 4. Композиция по п. 1, в которой диспергированная фаза содержит вещество, выбранное из группы, состоящей из проп-2-енил 3-циклогексилпропаноата, (4aR,5R,7aS,9R)-октагидро-2,2,5,8,8,9а-гексаметил-4h-4а,9-метаноазулено(5,6-d)-1,3диоксола, (3aR,5aS,9aS,9bR)-3а,6,6,9а-тетраметил-2,4,5,5а,7,8,9,9b-октагидро-1Н-бензо [е][1]бензофурана, 4-метоксибензальдегида, бензил-2-гидроксибензоата, 2метоксинафталина, 3-(4-трет-бутилфенил)пропаналя, 3а,6,6,9а-тетраметил2,4,5,5а,7,8,9,9b-октагидро-1Н-бензо[е][1]бензофурана, 3,7-диметилокт-6-ен-1-ола, 3,7диметилокт-6-еннитрила, 3-(4-трет-бутилфенил)бутаналя, 3-(4-пропан-2-илфенил) бутаналя, (Е)-1-(2,6,6-триметил-1-циклогекса-1,3-диенил)бут-2-ен-1-она, деканаля, (Е) -1-(2,6,6-триметил-1-циклогекс-3-енил)бут-2- ...

Stabilized droplets for calibration and testing

Provided herein, are droplet mixture compositions and systems and methods for forming mixtures of droplets. The system may comprise two or more droplet generation units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The combined droplet populations can be mixed, heated, and collected for multiple uses, such as for use as calibration standards for instrument testing and analysis.

Moving micro valve

(57)【要約】 ミクロチャンネルを通してのミクロ液滴の移動及び混合がミクロ規模装置を用いて記述される。サンプル及び試薬を流入口（A）を通して装置に注入し、それらを別々の液滴としてチャンネル（B）を通して反応チャンバ、例えば、混合及び反応（例えば、制限酵素消化又は核酸増幅）が行われる熱的に制御された反応器（C）に輸送する。次に、それらの生化学的生成物を同じ方法によって電気泳動モジュール（D）に移動させ、そこで移動データを検出器（E）によって集めて記録器機に送信する。重要なことには、これらの流体及び電気構成成分は機能及び構成が生物学的反応及び試薬に完全に適合するように設計されている。

System and methodology for chemical constituent sensing and analysis

A technique facilitates detection and analysis of constituents, e.g. chemicals, which may be found in formation fluids and/or other types of fluids. The technique comprises intermittently introducing a first fluid and a second fluid into a channel in a manner which forms slugs of the first fluid separated by the second fluid. The intermittent fluids are flowed through the channel to create a mixing action which mixes the fluid in the slugs. The mixing increases the exchange, e.g. transfer, of the chemical constituent between the second fluid and the first fluid. The exchange aids in sensing an amount of the chemical or chemicals for analysis. In many applications, the intermittent introduction, mixing, and measuring can be performed in a subterranean environment.

Thermal microvalves

The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

Fluid injection

The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.

Thermal microvalves

Thermal microvalves and their method of use include a fusible material (40) positioned in a microchannel (44). A heater (42) melts the material (40) which is then forced by air pressure (46) into a second channel (48) where it cools and solidifies, blocking flow. Alternatively, the melted and solidified material (40) can hold a diaphragm of a diaphragm valve in place, eliminating the need for continued applied force on the diaphragm.

Thermal microvalves

System for the encapsulating active substance in drop and other compartments

本发明整体涉及微粒，尤其涉及用于在微粒内包封的体系和方法。在一个方面，本发明整体涉及其中包含实体的微粒，其中所述实体包含可通过例如扩散从微粒释放的试剂。在一些情况下，可在不破坏微粒的情况下将试剂从微粒释放。所述实体可为例如聚合物颗粒、水凝胶颗粒、流体液滴等。所述实体可被包含在流体内，所述流体继而被包封在微粒内。所述试剂可从实体释放到流体中，然后从流体通过微粒释放。以此类方式，可在例如相对长的时间尺度上控制试剂从微粒的释放。本发明的其它实施方案整体涉及制备此类微粒的方法，使用此类微粒的方法，用于制备此类微粒的微流体装置等。

Microchannel device and method pertaining thereto

Verteilungsvorrichtung für einen kapillarreaktor und verfahren

Apparatus and process for producing patterned, micron and nanometer size reaction and mixing zones for fluids deposited on smooth, rough and porous surfaces and applications of that process

Process for producing patterned, micron and nanometer scale features by reacting or mixing in the small volumes at the intersections of coalescing drops, at the fronts of colliding thin films in front of spreading drops, or in the pore space of a porous medium under the drop. The process can be implemented on smooth, rough or porous surfaces and embodiments include multiplexed, single drop chemical or biochemical sensors and encryption of information of a printed page.

Thermische mikroventile

マイクロ流路装置及びこれに関する方法

【課題】微量液体の効果的な操作を実現するマイクロ流路装置及びこれに関する方法を提供する。 【解決手段】本発明に係るマイクロ流路装置１は、上流液体プラグ及び気体を流すための上流流路部（１０）と、下流液体プラグ及び気体を流すための下流流路部（２０）と、前記上流流路部の下流端部と前記下流流路部の上流端部との間に設けられ主液体プラグを保持するための液体保持部（３０）と、前記上流流路部の前記下流端部から前記下流流路部の前記上流端部まで前記液体保持部を迂回するように設けられ、前記液体保持部が前記主液体プラグを保持した状態で気体を流すための気体バイパス流路部（４０）と、を備えている。 【選択図】図２Ａ

Microchannel device and method pertaining thereto

A microchannel device includes: an upstream channel portion configured to allow an upstream liquid plug and a gas to flow therethrough; a downstream channel portion configured to allow a downstream liquid plug and a gas to flow therethrough; a liquid holding portion provided between a downstream end portion of the upstream channel portion and an upstream end portion of the downstream channel portion, the liquid holding portion being configured to hold a main liquid plug therein; and a gas bypass channel portion provided so as to bypass the liquid holding portion from the downstream end portion of the upstream channel portion to the upstream end portion of the downstream channel portion, the gas bypass channel portion being configured to allow the gas to flow therethrough in a state in which the liquid holding portion holds the main liquid plug.

Dispositivo de microcanal y procedimiento relacionado con el mismo

Un procedimiento que comprende lo siguiente (a) a (g): (a) proporcionar un dispositivo de microcanal que comprende una porción de canal corriente arriba, una porción de canal corriente abajo, una porción de retención de líquidos provista entre una porción final corriente abajo de la porción de canal corriente arriba y una porción final corriente arriba de la porción de canal corriente abajo, y una porción de canal de derivación de gas que se proporciona para derivar la porción de retención de líquido desde la porción final corriente abajo de la porción de canal corriente arriba a la porción final corriente arriba de la porción de canal corriente abajo; (b) llenar la porción de canal de derivación de gas con un gas y mantener un tapón de líquido principal en la porción de retención de líquido; (c) hacer que un primer tapón de líquido corriente arriba y un segundo tapón de líquido corriente arriba fluyan secuencialmente a través de la porción de canal corriente arriba hacia la porción de retención de líquido; (d) hacer que el primer tapón de líquido corriente arriba fluya hacia la porción de retención de líquido para fusionar el primer tapón de líquido corriente arriba con el tapón de líquido principal, y empujar una parte del tapón de líquido principal después de la fusión a la porción de canal corriente abajo; (e) hacer que un gas que sigue al primer tapón de líquido corriente arriba fluya hacia la porción de canal de derivación de gas para expulsar una parte del gas en la porción de canal de derivación de gas a la porción final corriente arriba de la porción de canal corriente abajo, para cortar la parte del tapón de líquido principal, que se empuja en el ítem (d), para formar un primer tapón de líquido corriente abajo; (f) hacer que el segundo tapón de líquido corriente arriba fluya hacia la porción de retención de líquido para fusionar el segundo tapón de líquido corriente arriba con el tapón de líquido principal y empujar una parte del tapón de líquido principal ...

マイクロ流路装置及びこれに関する方法

　微量液体の効果的な操作を実現するマイクロ流路装置及びこれに関する方法を提供する。本発明に係るマイクロ流路装置（１）は、上流液体プラグ及び気体を流すための上流流路部（１０）と、下流液体プラグ及び気体を流すための下流流路部（２０）と、前記上流流路部の下流端部と前記下流流路部の上流端部との間に設けられ主液体プラグを保持するための液体保持部（３０）と、前記上流流路部の前記下流端部から前記下流流路部の前記上流端部まで前記液体保持部を迂回するように設けられ、前記液体保持部が前記主液体プラグを保持した状態で気体を流すための気体バイパス流路部（４０）と、を備えている。

一种电化学发光微乳液及生成方法、生成装置与应用

本发明公开了一种电化学发光微乳液及生成方法、生成装置与应用，包括外相溶液；所述外相溶液包裹中间相溶液；所述中间相溶液包裹内相溶液；所述外相溶液、中间相溶液和内相溶液的体积比为4～20：1.25：1；所述外相溶液为含5wt％PVA的水溶液、矿物油或含2wt％tween 20的水溶液；所述中间相溶液为含2wt％氟表面活性剂的FC 40、含2wt％氟表面活性剂的HFE 7500或含15wt％甘油的水溶液；所述内相溶液为藻蓝素、胆绿素、中胆绿素或尿胆素。本发明的生成装置可以对流速进行调节，生成不同直径的电化学发光微乳液，直径80μm‑200μm可调，且电化学发光微乳液在电致化学发光平台中检测出较强的电流信号；并且定量检测甲胎蛋白检测的检测范围是0.05‑500ng/ml。

一种双层液滴生成装置及制造方法

本发明公开了一种双层液滴生成装置及制造方法，双层液滴生成装置包括内层液滴生成管、第一进样管、第二进样管、外层液滴生成管、第三进样管和注射部件，第一进样管穿设于内层管腔，第二进样管对接内层液滴生成管的第一上端口，内层液滴生成管穿设于外层管腔，第三进样管对接外层液滴生成管的第二上端口；注射部件分别与第一进样管、第二进样管、第三进样管连接；通过控制第一进样管、第二进样管或第三进样管的数量、内径或位置，以控制双层液滴的内液滴数量与尺寸。利用管道相互嵌套，从而在管道内实现了双层液滴的生成，此外，通过控制管道数量、利用不同内径的管道相互之间排列方式的不同，从而实现可以控制液滴的生成尺寸的效果。

Microchannel device and method pertaining thereto

A microchannel device includes: an upstream channel portion configured to allow an upstream liquid plug and a gas to flow therethrough; a downstream channel portion configured to allow a downstream liquid plug and a gas to flow therethrough; a liquid holding portion provided between a downstream end portion of the upstream channel portion and an upstream end portion of the downstream channel portion, the liquid holding portion being configured to hold a main liquid plug therein; and a gas bypass channel portion provided so as to bypass the liquid holding portion from the downstream end portion of the upstream channel portion to the upstream end portion of the downstream channel portion, the gas bypass channel portion being configured to allow the gas to flow therethrough in a state in which the liquid holding portion holds the main liquid plug.

一种基于不锈钢毛细管的流体交汇型微流控装置及液滴的制备方法

本发明涉及一种基于不锈钢毛细管的流体交汇型微流控装置及液滴的制备方法，属于微流控技术领域，该装置包括：连续相模块，其进液处设有与其连通的第一微量注射泵，第一微量注射泵用于注入连续相；设置于连续相模块内的不锈钢毛细管流道模块，其包括相连通的第一分散相毛细管和第二分散相毛细管，二者连通处为液滴形成部；第二微量注射泵，其与第一分散相毛细管或第二分散相毛细管的进液处相连通，第二微量注射泵用于注入分散相。与现有技术相比，本发明通过不锈钢毛细管的嵌套配合使用实现了微流控芯片三维同轴的快速组装；且使用不锈钢毛细管作为微流控装置的核心器件，极大降低了微流控装置的制造难度与制备成本。

一种基于不锈钢毛细管的流体交汇型微流控装置及液滴的制备方法

本发明涉及一种基于不锈钢毛细管的流体交汇型微流控装置及液滴的制备方法，属于微流控技术领域，该装置包括：连续相模块，其进液处设有与其连通的第一微量注射泵，第一微量注射泵用于注入连续相；设置于连续相模块内的不锈钢毛细管流道模块，其包括相连通的第一分散相毛细管和第二分散相毛细管，二者连通处为液滴形成部；第二微量注射泵，其与第一分散相毛细管或第二分散相毛细管的进液处相连通，第二微量注射泵用于注入分散相。与现有技术相比，本发明通过不锈钢毛细管的嵌套配合使用实现了微流控芯片三维同轴的快速组装；且使用不锈钢毛细管作为微流控装置的核心器件，极大降低了微流控装置的制造难度与制备成本。

一种双层液滴生成装置及制造方法

本发明公开了一种双层液滴生成装置及制造方法，双层液滴生成装置包括内层液滴生成管、第一进样管、第二进样管、外层液滴生成管、第三进样管和注射部件，第一进样管穿设于内层管腔，第二进样管对接内层液滴生成管的第一上端口，内层液滴生成管穿设于外层管腔，第三进样管对接外层液滴生成管的第二上端口；注射部件分别与第一进样管、第二进样管、第三进样管连接；通过控制第一进样管、第二进样管或第三进样管的数量、内径或位置，以控制双层液滴的内液滴数量与尺寸。利用管道相互嵌套，从而在管道内实现了双层液滴的生成，此外，通过控制管道数量、利用不同内径的管道相互之间排列方式的不同，从而实现可以控制液滴的生成尺寸的效果。

一种电化学发光微乳液及生成方法、生成装置与应用

本发明公开了一种电化学发光微乳液及生成方法、生成装置与应用，包括外相溶液；所述外相溶液包裹中间相溶液；所述中间相溶液包裹内相溶液；所述外相溶液、中间相溶液和内相溶液的体积比为4～20：1.25：1；所述外相溶液为含5wt％PVA的水溶液、矿物油或含2wt％tween 20的水溶液；所述中间相溶液为含2wt％氟表面活性剂的FC 40、含2wt％氟表面活性剂的HFE 7500或含15wt％甘油的水溶液；所述内相溶液为藻蓝素、胆绿素、中胆绿素或尿胆素。本发明的生成装置可以对流速进行调节，生成不同直径的电化学发光微乳液，直径80μm‑200μm可调，且电化学发光微乳液在电致化学发光平台中检测出较强的电流信号；并且定量检测甲胎蛋白检测的检测范围是0.05‑500ng/ml。

Methods for encapsulation of actives within droplets and other compartments

The present invention generally relates to microparticles and, in particular, to systems and methods for encapsulation within microparticles. In one aspect, the present invention is generally directed to microparticles containing entities therein, where the entities contain an agent that can be released from the microparticles, e.g., via diffusion. In some cases, the agent may be released from the microparticles without disruption of the microparticles. The entities may be, for instance, polymeric particles, hydrogel particles, droplets of fluid, etc. The entities may be contained within a fluid that is, in turn, encapsulated within the microparticle. The agent may be released from the entity into the fluid, and then from the fluid through the microparticle. In such fashion, the release of agent from the microparticle may be controlled, e.g., over relatively long time scales. Other embodiments of the present invention are generally directed to methods of making such microparticles, methods of using such microparticles, microfluidic devices for making such microparticles, and the like.

Fluid injection

The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.

Compositions comprising encapsulated actives within droplets and other compartments

The present invention generally relates to microparticles and, in particular, to systems and methods for encapsulation within microparticles. In one aspect, the present invention is generally directed to microparticles containing entities therein, where the entities contain an agent that can be released from the microparticles, e.g., via diffusion. In some cases, the agent may be released from the microparticles without disruption of the microparticles. The entities may be, for instance, polymeric particles, hydrogel particles, droplets of fluid, etc. The entities may be contained within a fluid that is, in turn, encapsulated within the microparticle. The agent may be released from the entity into the fluid, and then from the fluid through the microparticle. In such fashion, the release of agent from the microparticle may be controlled, e.g., over relatively long time scales. Other embodiments of the present invention are generally directed to methods of making such microparticles, methods of using such microparticles, microfluidic devices for making such microparticles, and the like.