КОНТЕЙНЕР ДЛЯ ИЗОЛЯЦИИ И ИДЕНТИФИКАЦИИ МИКРООРГАНИЗМА

Изобретение относится к биотехнологии. Предложен контейнер для изоляции и идентификации микроорганизма. Контейнер включает верхнюю часть, имеющую широкий внутренний диаметр, и нижнюю часть, имеющую капиллярную трубку, среднюю коническую часть, соединяющую указанные верхнюю и нижнюю части, оптическое окно на дне и/или на одной или более чем одной стенке контейнера. Оптическое окно имеет толщину менее 0,1 дюйма (2,54 мм) и является прозрачным для длин волн ближнего инфракрасного, видимого и/или ультрафиолетового светового спектра. Окно содержит кварц, кварцевое стекло, сапфир, акриловую смолу, метакрилат, сополимер циклического олефина, полимер циклоолефина или любую их комбинацию. Капиллярная трубка имеет внутренний диаметр от 0,001 дюйма (0,03 мм) до 0,04 дюйма (1,02 мм). Контейнер обеспечивает изоляцию из гемокультуры и других комплексных образцов микроорганизмов, которые свободны от интерферирующих материалов и совместимы с технологиями быстрой идентификации. 7 з.п. ф-лы, 12 ил.,1 пр.

Устройства детектирования света с защитной облицовкой и относящиеся к ним способы

Изобретение относится к области измерительной техники и касается устройства детектирования света. Устройство содержит реакционную структуру, образующую множество реакционных углублений для вмещения реакционного раствора и реакционного центра, основу устройства, расположенную под реакционной структурой и содержащую множество датчиков света, схему устройства, электрически соединенную с датчиками света, облицовочный слой, проходящий вокруг каждого световода и расположенный между каждым световодом и схемой устройства и защитный слой, проходящий вокруг каждого световода и расположенный между каждым световодом и облицовочным слоем. Реакционный центр выполнен с возможностью генерирования световых излучений под действием света возбуждения после обработки реакционным раствором. Световоды проходят в основу устройства от вводных областей в направлении соответствующего датчика света. Защитный слой предотвращает взаимодействие между реакционным раствором, проходящим через реакционную структуру и световод ...

КОНТРОЛЬ КАЧЕСТВА/РАБОТЫ УСТРОЙСТВА ЛАТЕРАЛЬНОГО ПРОТОЧНОГО АНАЛИЗА НА ОСНОВАНИИ МОНИТОРИНГА ПОТОКА

... 1. Способ обеспечения контроля качества работы устройства латерального проточного анализа, причем упомянутое устройство содержит подложку, содержащую множество отдельных зон, включая по меньшей мере одну зону для нанесения образца, по меньшей мере одну зону обнаружения ниже по потоку от упомянутой по меньшей мере одной зоны для нанесения образца, и по меньшей мере одну отводящую зону ниже по потоку от упомянутой по меньшей мере одной зоны обнаружения, причем каждая из упомянутых зон соединена по текучей среде с остальными зонами вдоль пути потока жидкости, по которому образец течет под действием капиллярных силиз упомянутой зоны для нанесения образца к упомянутой отводящей зоне, причем упомянутый способ включает этапы:нанесения образца на зону для нанесения образца;смешивания образца и реагента, причем образец и реагент могут смешиваться перед нанесением образца на зону для нанесения образца или на устройство анализа, причем упомянутый реагент содержит по меньшей мере одно детекторное вещество ...

СЕПАРАТОР ДЛЯ РАЗДЕЛЕНИЯ, ХАРАКТЕРИСТИКИ И(ИЛИ) ИДЕНТИФИКАЦИИ МИКРООРГАНИЗМОВ

... 1. Контейнер для изоляции и идентификации микроорганизма, включающий:(a) верхнюю часть, имеющую широкий внутренний диаметр;(b) нижнюю часть, имеющую узкий внутренний диаметр; и(c) оптическое окно на дне, сверху и/или на одной или более чем одной стенке контейнера, которое является прозрачным по меньшей мере для части ближнего инфракрасного, видимого и/или ультрафиолетового светового спектра.2. Контейнер по п.1, который имеет объем, достаточный, чтобы удержать достаточное количество образца для получения осадка микроорганизмов.3. Контейнер по п.1, где верхняя часть и нижняя часть соединены средней конической частью.4. Контейнер по п.1, где нижняя часть составляет менее половины общей высоты контейнера.5. Контейнер по п.1, который включает устройство для закрывания или имеет резьбу для присоединения устройства для закрывания, так чтобы контейнер мог быть герметичным.6. Контейнер по п.1, где оптическое окно состоит из кварца, кварцевого стекла, сапфира, акриловой смолы, метакрилата, сополимера ...

TESTGERÄT ZUR DURCHFÜHRUNG VON NUKLEINSÄURE-AMPLIFIKATIONSREAKTIONEN

Kontinuierliches, kapazitätsgestütztes Überwachen von Flüssigkeitsströmen in einer mikrofluidischen Einheit

Ein mikrofluidischer Chip (10) weist einen Mikrokanal (11, 11a), der mit einer Flüssigkeit (5) füllbar ist, wobei der Mikrokanal (11, 11a) ein Elektrodenpaar (21, 22, 21a, 22a) und einen Flüssigkeitsströmungsweg (12) aufweist, der zwischen den Elektroden (21, 22, 21a, 22a) definiert ist, wobei sich jede der Elektroden (21, 22, 21a, 22a) entlang des Strömungsweges (12) und parallel zu einer Richtung einer Flüssigkeit (5) erstreckt, die im Betrieb jeden der Mikrokanäle (11, 11a) füllt, und eine elektrische Schaltungsanordnung auf, die mit jeder der Elektroden (21, 22, 21a, 22a) verbunden ist und konfiguriert ist, um im Betrieb über die Elektroden (21, 22, 21a, 22a) eine Kapazität der Elektroden, die mit einer Flüssigkeit (5) benetzt sind, die den Strömungsweg (12) kontinuierlich füllt, als eine Funktion der Zeit zu messen.

ELASTOMERISCHE MIKROPUMPEN- UND MIKROVENTILSYSTEME

Screening-Testsystem mit hohem Durchfluss durch mikrofluidische Vorrichtungen

Flüssigkeitsströmungskontrolle in gebogenen Kapillarkanälen

Device fo9r use in fluid array

Fluidic separation and detection

Cell migration assay

Capillary polymerase chain reaction

A reactor for the polymerase chain reaction (pcr) comprises a capillary, preferably a plurality of capillaries having a common entrance and common exit. A reaction solution is passed therethrough and subject to the steps of melting, primer attachment and amplification (construction) at three different temperatures in either three separate zones or in one zone. The flow rate of the reactants in the construction zone is maintained below ten capillary diameters per second. Preferably, the silicon microfabricated capillaries are coated with conventional chromatographic coatings such a carbowaxes, lipoproteins or glycoproteins and contain high thermal conductivity metal powders such as silver. High frequency AC heating elements combined with cooling plates allow rapid temperature changes; each temperature stage may be passed through in 0.25 of a second. It is envisaged that 30 cycles are completed within 2.5 minutes.

Reaction plate

Fluid flow control in a microfluidic device

The invention relates to controlling a flow of fluids in a microfluidic device (10), the microfluidic device (10) having a fluid channel (140), comprising applying a magnetic field to at least a part of the fluid channel (141, 142) to force at least a portion of a first fluid to retain in at least a portion of the fluid channel (140).

Amplifying nucleic acids using microfluidic device to perform PRC

The amplification, of for example DNA, occurs within micro-fluidic device 10 having a space 32 filled with a gel 54. Reagents for carrying out a polymerase chain reaction (PCR) are supported within the gel matrix 54. A nucleic acid containing sample is brought into contact with the gel 54, by being placed in sample collection well 34. The sample comprises whole cells or cell lysate without any prior separation of the nucleic acid from other components of the cells. PCR amplification of nucleic acid from the sample is performed in the space 32 using the PCR reagents. A liquid receiving well 24 extends from an opening 46 to the gel 50 containing channel 40. A liquid contactable electrode 48 is receivable within the liquid receiving well 24. The micro-fluidic device is held and located by a detection apparatus.

A device for carrying out cell lysis and nucleic acid extraction

Microfluidic probe head for providing a sequence of separate liquid volumes separated by spacers

Effects of space travel on human brain cells

Liquid handling

Droplet actuation

Effects of space travel on human brain cells

A method of perfusing cells comprising: a) providing a fluid source, said fluid source in fluidic communication with a microfluidic device having at least one microchannel comprising surfaces, wherein at least one surface comprise living vascular cells; and b) contacting said vascular cells with said fluid at a flow rate of between 300 and 1500 μl per hour; and optionally, c) further comprising collecting at least a portion of the fluid that exits said microfluidic device; and optionally said flow rate of step b) is applied for at least 6 days. The cells may be brain endothelial cells, derived from fibroblasts and be human cells. A method of seeding such vascular cells in a microfluidic device is further claimed.

Apparatus for determining the presence of a contaminant in a sample of water or other fluid.

NANO-PCR: Methods and devices for nucleic acid amplification and detection

Apparatus for determining the presence of a contaminant in a sample of water or other fluid

Nano-pcr: methods and devices for nucleic acid amplification and detection

Apparatus for determining the presence of a contaminant in a sample of water or other fluid

Apparatus for determining the presence of a contaminant in a sample of water or other fluid

Nano-pcr: methods and devices for nucleic acid amplification and detection

Nano-pcr: methods and devices for nucleic acid amplification and detection

ANALYSIS DEVICE

LIQUID FLOW CONTROL IN CURVED CAPILLARY CHANNELS

MICRO-FLUID ARTICLE

PROCEDURE FOR THE MOVEMENT OF SMALL LIQUID QUANTITIES IN MICRO-CHANNELS BY ACOUSTIC WAVES

BIOCHEMISTRY ON DROPLET BASIS

MICRO FLUIDIC DEVICE AND PROCEDURE FOR THE DETERMINATION OF THE LIQUID COAGULATING TIME

PYROSEQUENZIERUNG ON DROPLET BASIS

VOLUME DURCHFLUSSZYTOMETRIE ONE AND CELL ASSORTMENT

GENERAL-PURPOSE REACTION PLATE FOR ANALYSIS, IN PARTICULAR FOR ANALYSIS OF FULL BLOOD OR OF SOLID PARTICLE CONTAINING FLUIDS

ANALYSIS DEVICE

ANALYSIS DEVICE

ANALYSIS DEVICE

ANALYSIS DEVICE

ANALYSIS DEVICE

ANALYSIS DEVICE

Microfabricated elastomeric valve and pump systems

Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor

A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Container and calibration standard plate

Provided is a container (10) including a base material (1) including a plurality of concave portions (2); a recognition unit (3) disposed on the base material and configured to recognize the base material; and a storage unit (4) disposed in a position other than a measurement region of the base material and configured to store information on biomaterials contained in the plurality of concave portions, wherein the recognition unit and the storage unit are allowed to correspond to each other.

DEVICES, SYSTEMS AND METHODS FOR EVALUATION OF HEMOSTASIS

Provided are devices, systems and methods for evaluation of hemostasis. Also provided are sound focusing assemblies.

System and methods for selective effluent collection

A system may include a flow cell through which a plurality of reagents may be pumped during a genetic sequencing operation, an effluent line that, in operation, is to conduct a used reagent, a used reagent valve to receive the used reagent from the effluent line and controllable to select one of a plurality of disposal paths for the used reagent, and control circuitry coupled to the used reagent valve that, in operation, is to control the used reagent valve to select a desired one of the disposal paths depending upon which reagent is being pumped though the flow cell.

Liquid handling system and method

A liquid handling system (1) and method, e.g., for testing blood samples. The system comprises a cartridge (3), and a transfer device (5) couplable to a liquid reservoir (7). The cartridge (3) comprises compartments (11) with an inlet (15), closed by a seal, and an outlet (17), closed by a gas- permeable liquid-tight filter. Keying portions (3A, 5A) define a relative position and orientation of the cartridge and the transfer device. Penetrating elements (27) of the transfer device (5) penetrate the seal of each compartment (11), the penetrating elements having lumina for fluidly connecting the reservoir (7) and each compartment cavity (13) of the cartridge.

DEVICES, SYSTEMS AND METHODS FOR EVALUATION OF HEMOSTASIS

Provided are devices, systems and methods for evaluation of hemostasis. Also provided are sound focusing assemblies. WO 2013/105986 PCT/US2012/025270 __~~ ~FG IF '~1y_ Bi il 116 114 112 110 FI.1 -ill.

Microfluidic protein crystallography

Nano-PCR: methods and devices for nucleic acid amplification and detection

Method for producing an analytical magazine

A method for producing an analytical magazine (110) is proposed. The analytical magazine (110) is designed for receiving a plurality of analytical aids (134) in a plurality of chambers (122). The method comprises the following steps: - providing at least a first component (112) of the analytical magazine (110), wherein the first component (112) comprises a plurality of receptacles (120), - providing a plurality of analytical aids (134), wherein the analytical aids (134) are connected to one another, and preferably aligned in relation to one another, by at least one holding element (144), - introducing the analytical aids (134) into the receptacles (120); and - separating the analytical aids (134) from the holding element (144).

Separation device for use in the separation, characterization and/or identification of microorganisms

EFFICIENT MMICROFLUIDIC DEVICES

Methods and devices for high throughput fluid delivery

Open substrate platforms suitable for analysis of biomolecules

DEVICE FOR HANDLING LIQUIDS CONTAINED IN A PLURALITY OF CHANNELS

Plurality of reaction chambers in a test cartridge

PLURALITY OF REACTION CHAMBERS IN A TEST CARTRIDGE A fluidic testing system (100), comprising a plurality of test chambers (116), each having a wall that defines a longest side of a given test chamber (116), wherein each of the plurality of test chambers (116) has only one opening (304) being through the wall of a corresponding test chamber (116), and wherein the wall is aligned substantially parallel to a gravity vector; a plurality of inlet channels (210), wherein each of the plurality of test chambers (116) is coupled via its respective opening (304) to only one of the plurality of inlet channels (302); and a fluidic network configured to connect the plurality of inlet channels (210) to one or more other chambers (116).

Microfluidics sorter for cell detection and isolation

A microfluidic device is disclosed. The device comprises at least one inlet for receiving circulating tumor cells and other cells in a sample; at least one curvilinear and/or spiral channel through which the sample is caused to undergo partial or complete Dean cycles to isolate the circulating tumor cells from the other cells; and at least one outlet configured to communicate with the channel for providing the isolated circulating tumor cells. The channel is configured to provide a predetermined Force ratio based on a desired threshold cell size of the circulating tumor cells. A corresponding method of manufacturing of the device, and a related diagnostic system are also disclosed.

Sample holder and method of using the same

Thermal cycling reaction block and continuous real-time monitoring apparatus using the same

Provided is a real-time monitoring apparatus, including a thermal cycling reaction block having heating block which is formed of a hollow part and divided by an insulating layer, and a capillary tube through which a sample is flowed in and/or out and which is wound on the heating block so that different temperatures are transferred and thus reaction cycle is repeatedly performed a light source; a band pass filter; a condensing lens; a beam splitter a reflecting mirror which is rotatably connected with a motor so as to transfer the excitation light reflected from the beam splitter to the capillary tube and reflect the fluorescence generated from the sample in the capillary tube; and a fluorescence detecting part.

Microfluidic devices and fabrication

Methods for mass production of new microfluidic devices are described. The microfluidic devices may include an array of micro-needles with open channels in fluid communication with multiple reservoirs located within a substrate that supports the micro-needles. The micro-needles are configured so as to sufficiently penetrate the skin in order to collect or sample bodily fluids and transfer the fluids to the reservoirs. The micro-needles may also deliver medicaments into or below the skin.

System and method for movement and timing control

The present invention relates to fluidic systems for controlling one or more fluids and/or one or more reagents. These systems can be used in combination with one or more devices for assaying, processing, and/or storing samples. In particular, the systems and related methods can allow for dispensing fluid in a controlled manner and/or introducing pause(s) when implementing assays or processes.

Open-top microfluidic devices and methods for simulating a function of a tissue

A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

Test card with well shelf

Provided herein are a test card, method of manufacturing the same, and mold for manufacturing the same. The test card may include a body and a well. The body may include a first surface and a second surface opposite the first surface. The well may define an opening extending from the first surface to the second surface and configured to receive a sample therein. The well may further include a shelf extending into the opening, such that the opening is narrower between the shelf and the second surface than between the shelf and the first surface.

Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use

A microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same

LIQUID TRANSFER DEVICE

Methods and apparatuses for analyzing polynucleotide sequences

STRUCTURAL UNITS THAT DEFINE FLUIDIC FUNCTIONS

A microfluidic device that comprises several microchannel structures in which there are an inlet port, an outlet port and therebetween a substructure comprising a fluidic function. The device has an axis of symmetry around which the microchannel structures are arranged as two or more concentric annular zones. for an inlet port and an outlet port of the same microchannel structure the inlet port is typically closer to the axis of symmetry than the outlet port. Each microchannel structure comprises a substructure that can retain liquid while the disc is spun around the axis and/or the inlet ports are positioned separate from the paths waste liquid leaving open waste outlet ports will follow across the surface of the disc when it is spun. For the microchannel structures of an annular zones the corresponding substructures are at essentially at the same radial distance while corresponding substructures in microchannel structures of different annular zones are at different radial distances. The ...

SLIP CHIP DEVICE AND METHODS

A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

MICROFLUIDIC LARGE SCALE INTEGRATION

High-density microfluidic chips contain plumbing networks with thousands of micromechanical valves and hundreds of individually addressable chambers. These fluidic devices are analogous to electronic integrated circuits fabricated using large scale integration (LSI). A component of these networks is the fluidic multiplexor, which is a combinatorial array of binary valve patterns that exponentially increases the processing power of a network by allowing complex fluid manipulations with a minimal number of inputs. These integrated microfluidic networks can be used to construct a variety of highly complex microfluidic devices, for example the microfluidic analog of a comparator array, and a microfluidic memory storage device resembling electronic random access memories.

CHARACTERIZATION OF REACTION VARIABLES

A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized ...

MICROFLUIDIC SYSTEMS INCLUDING THREE-DIMENSIONALLY ARRAYED CHANNEL NETWORKS

The present invention provides, in certain embodiments, improved microfluidic systems and methods for fabricating improved microfluidic systems, which contain one or more levels of microfluidic channels. The inventive methods can provide a convenient route to topologically complex and improved microfluidic systems. The microfluidic systems provided according to the invention can include three-dimensionally arrayed networks of fluid flow paths therein including channels that cross over or under other channels of the network without physical intersection at the points of cross over. The microfluidic networks of the invention can be fabricated via replica molding processes, also provided by the invention, utilizing mold masters including surfaces having topological features formed by photolithography. The microfluidic networks of the invention are, in some cases, comprised of a single replica molded layer, and, in other cases, are comprised of two, three, or more replica molded layers that ...

DETECTION ARTICLE HAVING FLUID CONTROL FILM

A detection article including at least one fluid control film layer having at least one microstructured major surface with a plurality of microchannels therein. The microchannels configured for uninterrupted fluid flow of a fluid sample throughout the article. The film layer including an acquisition zone wherein portions of the plurality of microchannels draw the fluid sample into the plurality of microchannels through openings in the microchannels at least by spontaneous fluid transport. The film layer also including a detection zone in uninterrupted fluid communication with the acquisition zone along the microchannels with the detection zone including at least one detection element that facilitates detection of a characteristic of the fluid sample within at least one microchannel of the detection zone. The detection article may be formed from a plurality of film layers that are stacked to form a three- dimensional article. The detection zone may include a plurality of detection elements ...

NANOCHANNEL ARRAYS AND THEIR PREPARATION AND USE FOR HIGH THROUGHPUT MACROMOLECULAR ANALYSIS

Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

DEVICES FOR THE ANALYSIS OF FLUIDS AND CONTROLLED TRANSPORT OF FLUIDS

The invention relates to devices for the analysis of fluids and for other applications, comprising at least a filling section, an analysis section and a system of channels. It enables the efficient transport, transfer and movement of fluids. In particular, the invention provides fluidic microstructures for controlled transport and movement of fluids in said devices. Devices of the invention include one or more features that can enhance performance of the fluid transfer, referred to as a pre-shooter stop structure, a butterfly structure, a cascade structure, a waste chamber inlet, a capillary driven sample inlet chamber, a capillary stop structure, a bifurcation flow-through structure, and a hydrophobic vent.

SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS

A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: distributing a population of cells and a population of non-cell particles across the array of wells through the fluid reservoir to increase capture efficiency of individual cell-particle pairs within the array of wells, and processing the captured cell-particle pairs at the set of wells.

SYSTEM FOR MICROBIAL SPECIES DETECTION, QUANTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY IDENTIFICATION

Several microfluidic chips are used to significantly accelerate the time to identify and quantify microbes in a biological sample and test them for antibiotic resistance, particularly for urinary tract infections. A first microfluidic chip uses antibody or similar probes to identify and quantify any microbes present. The same or a similar chip uses antibody or similar probes to identify microbes with DNA or RNA known to indicate antibiotic resistance. Another microfluidic chip tests for antibiotic susceptibility of any microbes by growing them in very small wells in the presence of antibiotics, reducing the time required for such testing by as much as 95%. Another microfluidic chip runs traditional urinalysis or similar tests.

CONTAINER AND CALIBRATION STANDARD PLATE

Provided is a container (10) including a base material (1) including a plurality of concave portions (2); a recognition unit (3) disposed on the base material and configured to recognize the base material; and a storage unit (4) disposed in a position other than a measurement region of the base material and configured to store information on biomaterials contained in the plurality of concave portions, wherein the recognition unit and the storage unit are allowed to correspond to each other.

ASSAY DEVICE AND METHOD FOR ASSESSING BLOOD CELLS

The present invention relates to an assay device and its use in medical analytics, in particular a method for assessing blood or blood cells.

DISPOSABLE FLUIDIC CARTRIDGE AND COMPONENTS

Disclosed are cartridge components, cartridges, systems, and methods for isolating analytes from biological samples. In various aspects, the cartridge components, cartridges, systems, and methods may allow for a rapid procedure that requires a minimal amount of material from complex fluids.

EMBOSSED FILM BIOPROCESSING CONTAINERS AND INTEGRITY TESTING OF BIOPROCESSING CONTAINERS

A testing method that includes the steps of evacuating air from a container to a negative atmospheric pressure, the container being a collapsible, flexible container, and comprising at least two opposing flexible walls, wherein a surface of at least one of the walls internal to the container comprises a plurality of channels or recessed features on said at least one wall and monitoring a mass flow or a state of vacuum so as to determine the integrity of the container. The container can be of any size or conformation, with or without attached fittings.

INTEGRATED MICROFLUIDIC DEVICES

Integrated microfluidic devices comprising at least an enrichment channel (10) and a main electrophoretic flowpath (12) are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet (6). The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.

DEVICES AND METHODS FOR PROGRAMMABLE MICROSCALE MANIPULATION OF FLUIDS

The present invention is directed generally to devices and methods for controlling fluid flow in meso-scale fluidic components in a programmable manner. Specifically, the present invention is directed to an apparatus and method for placing two microfluidic components in fluid communication at an arbitrary position and time, both of which are externally defined. The inventive apparatus uses electromagnetic radiation to perforate a material layer having selected adsorptive properties. The perforation of the material layer allows the fluid communication between microfluidic components. Other aspects of this invention include an apparatus and method to perform volumetric quantitation of fluids, an apparatus to program arbitrary connections between a set of input capillaries and a set of output capillaries, and a method to transport fluid in centripetal device from a larger to a smaller radius. In addition, the present invention also is directed to a method to determine the radial and polar ...

DETECTING MULTIPLE TYPES OF LEUKOCYTES

Methods, systems, and apparatus for detecting multiple types of leukocyte s in a blood sample material. A fluid or gas sample may pass through a sieve -based detection system of a cartridge. Detection and analysis techniques ma y be applied to determine the relative distribution of multiple types of whi te blood cells in the sample.

FLUIDIC ANALYSIS AND SEPARATION

A method for analysing a component is provided. The method comprises the steps of: (iii) providing the electrophoretic or thermophoretic movement of the component into a second fluid flow; (iv) diverting a part of a first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, wherein the diverted part is a third fluid flow which comprises the component; (v) contacting the third fluid flow with a fourth fluid flow, such as to form a laminar flow; (vi) providing the diffusion of the component into the fourth fluid flows.

WASH THROUGH PIPETTOR

A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.

SYSTEMS, DEVICES, AND METHODS FOR BODILY FLUID SAMPLE COLLECTION

Bodily fluid sample collection systems, devices, and method are provided. The device may comprise a first portion comprising at least a sample collection channel configured to draw the fluid sample into the sample collection channel via a first type of motive force. The sample collection device may include a second portion comprising a sample container for receiving the bodily fluid sample collected in the sample collection channel, the sample container operably engagable to be in fluid communication with the collection channel, whereupon when fluid communication is established, the container provides a second motive force different from the first motive force to move a majority of the bodily fluid sample from the channel into the container.

METHODS OF MACROMOLECULAR ANALYSIS USING NANOCHANNEL ARRAYS

... ²Methods of analyzing features such as the physical size of macromolecules or ²biomarkers along large ²genomic DNA molecules were disclosed as well as the devices for carrying out ²such high throughput ²analysis in a massively parallel fashion. Methods of fabricating such devices ²are also disclosed. The ²sample embodiment shown in the figure illustrates detection of a macromolecule ²flowing through a ²nanochannel device where passage of the macromolecule through the nanochannel ²is recorded by ²exciting features of interest to fluoresce with an excitation source, and then ²sensing the fluorescence ²with a photon detection device, the resulting fluorescent signal being ²correlated along the length of the ²macromolecule.² ...

TEST CHIP WITH PLUG FOR MEASURING THE CONCENTRATION OF AN ANALYTE IN A LIQUID, HOUSING FOR TEST CHIP AND SOCKET FOR PLUG

Measurement device (10) for taking a liquid sample, comprising: a measure ment portion (15) with a measurement surface (20), for being in use contacte d with the liquid surface and a plug portion (40) having a plurality of elec trical contacts (50), wherein the plug portion (40) is mountable to a socket (110) of a measurement evaluation apparatus (100).

METHODS AND APPARATUS FOR FLOW-CONTROLLED WETTING

Methods of determining a first position at which a dispersed phase droplet wets a surface of a channel are provided herein. The methods include immersing the dispersed phase droplet in a continuous phase fluid, wherein the continuous phase fluid is immiscible with the dispersed phase droplet, subsequently flowing the dispersed phase droplet in the continuous phase through the channel at a dispersed phase droplet velocity, wherein the dispersed phase droplet is separated from the surface by a film of the continuous phase fluid having a film thickness, and reducing the film thickness to rupture the film at the first position, wherein the droplet wets the surface at the first position.

HIGH-THROUGHPUT SCREENING ASSAY SYSTEMS IN MICROSCALE FLUIDIC DEVICES

The present invention provides microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems. The device includes a series of channels (110, 112), and optional reagent channel (114), fabricated into the surface of the substrate. At least one of these channels will typically have very small cross-sectional dimensions, e.g. in the range of from about 0.1 hem to about 500 .mu.m. The device also includes reservoirs (104, 106 and 108), disposed and fluidly connected at the ends of the channels (110 and 114). As shown, sample channel (112) is used to introduce the plurality of different test compounds into the device. As such, this channel will generally be fluidly connected to a source of large numbers of separate test compounds that will be individually introduced ...

MICROFLUIDIC DEVICES INCORPORATING IMPROVED CHANNEL GEOMETRIES

A microfluidic device comprising: a main analysis channel (100); a sample loading channel (102), sample loading channel (102) forming at intersection (108) with main analysis channel (100), sample introduction channels (104, 106) in fluid communication with sample loading channel (102) on opposite sides of intersection (108); first and second sample source (110 and 112) in fluid communication with sample introduction channels (104, 106); load/waste channels (114, 116) in fluid communication with sample loading channel (102) on opposite sides of intersection (108); and load/waste reservoirs (118, 120) in fluid communication with load/waste channels (114, 116).

Probebehälteraufnahmevorrichtung und Kalorimeter.

Eine erfindungsgemässe Probebehälteraufnahmevorrichtung (30) enthält ein Grundelement (31), welches zur Aufnahme eines Probebehälters ausgebildet ist, wobei das Grundelement (31) eine Ausnehmung (32) enthält, welche zur Aufnahme des Probebehälters ausgebildet ist. Das Grundelement (31) umfasst eine Grundfläche (33) und eine Deckfläche (36), wobei die Grundfläche (33) gegenüberliegend zur Deckfläche (36) angeordnet ist. Die Ausnehmung (32) erstreckt sich von der Grundfläche (33) zur Deckfläche (36), wobei die Grundfläche (33) und die Deckfläche (36) in einem Abstand (H) zueinander angeordnet sind. Die Ausnehmung (32) weist eine Ausnehmungsbreite (AB) und eine Ausnehmungslänge (AL) auf. Die Ausnehmungsbreite AB der Ausnehmung (32) auf einer der Grundflächen (33) oder Deckflächen (36) ist kleiner als in einer Zwischenfläche (37), welche sich zwischen der Grundfläche (33) und der Deckfläche (36) erstreckt. Die Erfindung betrifft auch ein Kalorimeter mit einer Aufnahme für Probebehälter.

Sample container receiving device and calorimeter.

Eine erfindungsgemässe Probebehälteraufnahmevorrichtung (30) enthält ein Grundelement (31), welches zur Aufnahme eines Probebehälters ausgebildet ist, wobei das Grundelement (31) eine Ausnehmung (32) enthält, welche zur Aufnahme des Probebehälters ausgebildet ist. Das Grundelement (31) umfasst eine Grundfläche (33) und eine Deckfläche (36), wobei die Grundfläche (33) gegenüberliegend zur Deckfläche (36) angeordnet ist. Die Ausnehmung (32) erstreckt sich von der Grundfläche (33) zur Deckfläche (36), wobei die Grundfläche (33) und die Deckfläche (36) in einem Abstand H zueinander angeordnet sind. Die Ausnehmung (32) weist eine Ausnehmungsbreite AB und eine Ausnehmungslänge AL auf. Die Ausnehmungsbreite AB der Ausnehmung (32) auf einer der Grundflächen (33) oder Deckflächen (36) ist kleiner als in einer Zwischenfläche (37), welche sich zwischen der Grundfläche (33) und der Deckfläche (36) erstreckt. Die Erfindung betrifft auch ein Kalorimeter mit einer Aufnahme für Probebehälter.

ИЗМЕРИТЕЛЬНЫЙ МИКРОЧИП С ВИЛКОЙ ДЛЯ ИЗМЕРЕНИЯ КОНЦЕНТРАЦИИ АНАЛИТА В ЖИДКОСТИ, КОРПУС ДЛЯ ИЗМЕРИТЕЛЬНОГО МИКРОЧИПА И РОЗЕТКА ДЛЯ ВИЛКИ

В изобретении предлагается измерительное устройство (10) для отбора жидкой пробы, которое содержит измерительный участок (15) с измерительной поверхностью (20), которая при использовании входит в контакт с поверхностью жидкости, и участок (40) вилки, имеющий множество электрических контактов (50), причем участок (40) вилки выполнен с возможностью установки в розетку (110) измерительного оценочного устройства(100).

Multi-task immunoaffinity device secured to a peripheral box and integrated to a capillary electrophoresis apparatus

The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as a modular, multi-task immunoaffinity device secured to a peripheral box and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. In one embodiment, two devices are coupled in-tandem to perform separately and sequentially microreactions and concentrations of proteins. In this embodiment, biological samples containing proteins can be subjected to proteolytic processing in the first device, and the resulting peptides subjected to selective purification and concentration in the second device.

Analysis reagent and analysis device having the analysis reagent carried therein

A reagent including a combination of a polyanionic compound and a bivalent cationic compound contains one substance selected from the group consisting of succinic acid, gluconic acid, alanine, glycine, valine, histidine, maltitol, and mannitol or at least one compound of the substance. A dry state of the reagent and deliquescence can be improved.

System and method for microfluidic flow control

A system and method for controlling fluid flow within a microchannel includes a fluid circuit comprising a fluid outlet well and one or more fluid inlet wells, all in communication with a microchannel. A negative pressure differential is applied to the outlet well and fluid flow from an inlet well into the microchannel is controlled by opening or closing the inlet well to atmospheric pressure. To stop fluid flow from the inlet well, a negative pressure differential may be applied to the inlet well to equalize pressure between the inlet and outlet wells. By sequentially opening and closing different inlet wells to atmosphere, controlled amounts of different reagents can be serially introduced into the microchannel.

Selective bond reduction in microfluidic devices

The invention overcomes the limitations described for the bonding of structured layers by providing a method for selectively reducing the bonding of materials. In its most generic form, the invention uses a bonding technique in combination with a printing method for modifying or covering at least one portion of a surface to either fully or partially prevent localised bonding. The structuring process may act upon the layers either before or after the bonding of the layers. The invention overcomes the limitations described in the application of affinity chromatography by providing a planar substrate with discrete optical detection flow cells that contain porous material and have connecting microchannels for fluid delivery and/or removal, and a method for making the same.

Three-dimensional (3d) hydrodynamic focusing using a microfluidic device

A microfluidic device comprises inlets for a sample flow and an out-of-plane focusing sheath flow, and a curved channel section configured to receive the sample flow and out-of-plane focusing sheath and to provide hydrodynamic focusing of the sample flow in an out-of-plane direction, the out-of-plane direction being normal to a plane including the curved channel. Examples of the invention also include improved flow cytometers.

Structures for controlling light interaction with microfluidic devices

Systems and methods for improved measurement of absorbance/transmission through fluidic systems are described. Specifically, in one set of embodiments, optical elements are fabricated on one side of a transparent fluidic device opposite a series of fluidic channels. The optical elements may guide incident light passing through the device such that most of the light is dispersed away from specific areas of the device, such as intervening portions between the fluidic channels. By decreasing the amount of light incident upon these intervening portions, the amount of noise in the detection signal can be decreased when using certain optical detection systems.

Microfabricated elastomeric valve and pump systems

A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Fluid controlling apparatus and method of controlling fluid by using the same

A fluid controlling apparatus including at least one sample chamber for holding a fluid containing target materials; a cleaning chamber for holding a cleaning solution; a first multi-port connected to the at least one sample chamber through a first channel and connected to the cleaning chamber through a second channel; a filter portion, connected to the first multi-port through a third channel, for filtering the target materials; and a first pump, connected to the filter portion, for applying a pressure; and a method of controlling a fluid using the fluid controlling apparatus, which comprises passing the fluid containing the target materials from the at least one sample chamber to the filter portion; and cleaning a path of the fluid by passing the cleaning solution through the path.

Microfluidic devices

The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

Microfluidic chip comprising several cylinder-piston arrangements

A microfluidic arrangement including a substrate in which a mircofluidic structure having several adjacent channels and at least one common supply line, into which the adjacent channels merge, is formed. Each of the adjacent channels form the cylinder of a cylinder-piston arrangement for receiving an associated piston. A method for producing the type of microfluidic-arrangement is also disclosed.

Microfluidic cartridge for processing and detecting nucleic acids

A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

Methods and apparatus for amplifying nucleic acids

A method of amplifying nucleic acid includes providing a microfluidic device having a space therein. The space is filled with a gel medium. Reagents for carrying out a PCR reaction are supported within the matrix of the gel medium within the space. A nucleic acid containing sample is brought into contact with the gel medium. The sample having whole cells or cell lysate without any prior separation of the nucleic acid from other components of the cells. PCR amplification of nucleic acid from the sample is performed in the space using the PCR reagents.

Suction Device for a Matrix Array of Pipette Tips

A suction device for a matrix array of pipette tips includes a tray configured to hold a liquid dispensed by the pipette tips. The tray includes an open-pore tray bottom. A suction tray cooperates with the open-pore tray bottom so as to form a vacuum chamber with the open-pore tray bottom forming a constituent of the vacuum chamber. A suction element is indirectly connected to the tray via the vacuum chamber so as to be configured to actively draw liquid over an entire surface into the vacuum chamber by suction via the tray bottom.

Mems based filter and catalizer

The present invention provides a filter for separating particles and/or catalyzer for particle reaction in a fluid. The device comprising array of passageways fabricated on a die wherein the passageway size is controlled by actuators. The passageway size is monitored and the actuators controlling the passageway size are activated conditionally upon the passageway size monitoring. Using movable actuators the passageway can achieve passageway size that is less then the fabrication minimal resolution. Proper locating, setting and/or activation of the actuators create passageways that can perform filtration of particles, trapping of particles and catalyzing particles reaction.

Microdroplet/bubble-producing device

The invention provides a microdroplet- or bubble-producing device that does not require separate through-holes for different liquid droplet/air bubble-producing flow channels. The droplet-producing flow channels are configured in a three-dimensional manner unlike in a conventional device where they are configured in a two-dimensional plane, and therefore the flow channels can be provided in a more high-density configuration than the prior art. In the microdroplet/bubble-producing device comprising slit(s) and the row of the plurality of microflow channels, the slit(s) is/are a continuous phase supply slit, a dispersion phase supply slit and a discharge slit, the plurality of microflow channels are configured so that the ends of the slit(s) and the two supply ports on both sides or the supply port and discharge port on either side are mutually connected, and at the sites of connection between the microflow channels and the slit(s), the dispersion phase undergoes shear with the continuous phase flow as the driving force, producing droplets or air bubbles of the dispersion phase, which are recovered from the discharge port.

Specimen Delivery Apparatus

A specimen delivery apparatus includes a housing having a backplane. The backplane includes at least one fluid communication port. The housing has an open state and a closed state. A midplane has a cavity for holding a sample. The midplane is sealed within the housing when the housing is in the closed state. A first actuator is disposed to move fluid within the housing when the housing is in a closed state and the first bulb is actuated. Various embodiments provide for caching of the fluid as well as staging to permit further preparation of the specimen prior to delivery. Various features roil the fluid to assist in extraction, mixing, and transport of the sample with the fluid. A destructible seal prevents fluid communication through the fluid communication port while the seal is intact. Communication of fluid through the fluid communication port is enabled only when the destructible seal is not intact. 1. A specimen delivery apparatus comprising:a housing including a backplane, wherein the backplane includes at least one fluid communication port, the housing having an open state and a closed state;a midplane having a cavity for holding a sample, wherein the midplane is sealed within the housing when the housing is in the closed state;a first bulb disposed to move fluid within the apparatus when the housing is in a closed state and the first bulb is actuated; anda destructible seal preventing fluid communication through the fluid communication port while the seal is intact, wherein actuation of the first bulb communicates fluid through the fluid communication port when the destructible seal is not intact.2. The apparatus of further comprising at least one attachment point for mechanically coupling the apparatus to another apparatus claim 1 , wherein the fluid communication port of the apparatus is aligned with the fluid communication port of the another apparatus to enable fluid communication between the apparatus and the another apparatus when coupled to each ...

SYSTEM AND METHOD FOR PERFORMING DROPLET INFLATION

The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet. 1. A system for performing droplet inflation , comprising at least one microfluidic channel , one or more fluid reservoirs , one or more inflators associated with each fluid reservoir , one or more inflator nozzles associated with each inflator , and a mechanism for disrupting an interface between a droplet and a fluid , wherein the at least one microfluidic channel comprises one or more droplets flowing therein , and wherein each fluid reservoir , inflator and inflator nozzle comprise at least one fluid therein (inflation fluid).2. A system according to claim 1 , wherein the at least one microfluidic channel intersects with each inflator nozzle at a region referred to as an inflation interface.3. A system according to claim 1 , wherein each fluid reservoir is associated with a series of in-line inflators.4. A system according to claim 3 , wherein the series of in-line inflators comprises at least one inflator nozzle associated with each inflator in the series.5. A system according to claim 2 , wherein a relatively controlled volume of fluid is inflated into one or more droplets at an inflation interface.6. A system according to claim 5 , wherein one or more droplets are inflated multiple times.7. A system according to claim 1 , wherein the volume of a ...

MICROFLUIDIC BASED INTEGRATED SAMPLE ANALYSIS SYSTEM

A portable microfluidic system capable of rapid diagnosis is described, which is able to analyze genetic, protein and cell composition of a sample in parallel for specific diseases from a relatively small sample. The method uses a single microfluidic chip integrated into a unique portable microfluidic platform and provides improved diagnostic accuracy, allows for frequent monitoring and is suitable for easy use in clinical settings. 1. A sample analysis system comprising: a sample input for applying a sample to the chip;', 'a separator connected to the sample input by a separation channel;', 'one or more reaction channels, each reaction channel being connected to the separator and being of varying length,', 'each said reaction channel being further connected to a respective outlet; and', 'a reagent inlet;, 'a microfluidic chip comprisinga thermoelectric semiconductor arranged to heat or cool one or more of said channels;an LED array arranged to emit radiation at a specific wavelength to the microfluidic chip;a detector arranged to detect fluorescence from said microfluidic chip; anda control unit arranged to control the valves and analyze results from the detector.2. The system according to wherein flow of the sample through the microfluidic chip is regulated by differential pressure.3. The system according to wherein the differential pressure is generated by the respective channels being of different lengths claim 2 , at least one of the channels including a valve for regulating flow along said one of the channels.4. The system according to wherein the separator is a gravity trap for separating blood into cells and plasma.5. The system according to including a cell reaction channel.6. The system according to including a PCR reaction chamber.7. The system according to including a protein detection channel.8. A microfluidic chip which comprises:a sample input for applying a sample to the microfluidic chip;a separator connected to the sample input by a separator ...

MAGNETIC SEPARATION DEVICE AND METHOD OF USE

The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation. 1. A device for separating biological entities comprising:a magnetic field source having a first surface and a second surface;a soft magnetic center pole having a first end contacting said first surface, and a tip end tapering away from said first end, thereby concentrating magnetic flux from said first surface towards said tip end;a first soft magnetic side pole having a first base end and a second end;a second soft magnetic side pole having a second base end and a third end;a channel contacting said tip end contains a fluidic sample comprising said biological entities, thereby causing separation of said biological entities bound with magnetic labels towards said tip end;wherein said first and second base ends contact said second surface;wherein said second and third ends are located on opposing sides of said tip end;wherein said magnetic flux from said tip end divides into each of said second and third ends, thereby forming a flux closure within said center pole and said first and second side poles; andwherein said biological entities bound with magnetic labels are removed from said channel after separating said channel from said tip end.2. The device according to claim 1 , wherein said magnetic field source comprises a permanent magnet having a first polarization surface contacting a top surface of a soft magnetic shield claim 1 , and a second polarization surface opposing said first polarization surface claim 1 , wherein said first surface is said second polarization surface and said second surface is said top surface.3. The ...

FLUIDIC DEVICE, SYSTEM, AND METHOD

A fluidic device includes: 1. A fluidic device , comprising:a first flow path in which two or more solutions are mixed; anda second circulation flow path in which a solution mixed in the first flow path is circulated and which has a capture part configured to capture a sample substance included in the solution and/or a detection part configured to detect a sample substance included in the solution.2. The fluidic device according to claim 1 , comprising:a connection flow path that directly or indirectly connects the first flow path and the second circulation flow path.3. The fluidic device according to claim 2 , whereinthe second circulation flow path has two or more circulation flow path valves, andthe connection flow path is connected to the circulation flow path at one partition, which is partitioned by closing any two of the circulation flow path valves, of the second circulation flow path.4. The fluidic device according to claim 1 , whereinthe second circulation flow path has two or more circulation flow path valves, and each of the circulation flow path valves is arranged such that each of circulation flow path partitions compartmentalized by a circulation flow path valve has a predetermined volume, and whereinat least one introduction flow path and at least one discharge flow path are connected to the second circulation flow path, and the introduction flow path and the discharge flow path are configured such that a different solution is capable of being introduced to each of the second circulation flow path partitions.5. The fluidic device according to claim 1 , whereina volume inside the second circulation flow path is smaller than a volume inside the first flow path.6. The fluidic device according to claim 1 , whereinthe first flow path is a first circulation flow path.7. The fluidic device according to claim 6 , whereinvolumes of a plurality of solutions are quantitatively determined in the first circulation flow path, and the quantitatively determined ...

MICROFLUIDIC CHIP FOR ANALYSIS OF CELL MOTILITY AND METHODS FOR USING SAME

The present invention describes an integrated apparatus that enables identification of migratory cells directly from a specimen. The apparatus only requires a small number of cells to perform an assay and includes novel topographic features which can reliably differentiate between migratory and non-migratory cell populations in a sample. Both the spontaneous and chemotactic migration of cancer cells may be measured to distinguish between subpopulations within a tumor sample. The migratory cells identified using the apparatus and methods of the present invention may be separated and further analyzed to distinguish factors promoting metastasis within the population. Cells in the apparatus can be treated with chemotherapeutic or other agents to determine drug strategies to most strongly inhibit migration. The use of optically transparent materials in some embodiments allows a wide range of imaging techniques to be used for in situ imaging of migratory and non-migratory cells in the apparatus. The apparatus and methods of the present invention are useful for predicting the metastatic propensity of tumor cells and selecting optimal drugs for personalized therapies. 1. A method for analysis of the motility of a population of cells in a sample comprising:a) adding to the inlet reservoir of the second channel of microchannel migration device a suspension of a population of cells from the sample;b) incubating the cells for a period time to allow the cells to fill the second channel;c) washing the inlet of the second channel;d) adding cell media to the one or more reservoirs of the one or more inlets of the first channel;e) imaging the cells in the apparatus for a period of time; andf) comparing the images of the cells in the device over time and identifying a cell or subpopulation of cells in the sample as migratory when the cell or subpopulation of cells migrates to the bifurcation of any of the migratory channels and/or enters one or more of the branch channels, and/or ...

Manipulation of Beads in Droplets and Methods for Manipulating Droplets

The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

DEVICES AND METHODS FOR SAMPLE CHARACTERIZATION

Devices and methods for characterization of analyte mixtures are provided. Some methods described herein include performing enrichment steps on a device before expelling enriched analyte fractions from the device for subsequent analysis. Also included are devices for performing these enrichment steps. 1. A method , comprising:introducing an analyte mixture into a microfluidic device containing a separation channel;applying an electric field across the separation channel to effect a separation of the analyte mixture;illuminating a first side of the microfluidic device through an optical slit;detecting light passing through the optical slit and the separation channel to monitor separation of the analyte mixture; andexpelling a fraction of the analyte mixture from an orifice in fluid communication with the separation channel.2. The method of claim 1 , wherein the microfluidic device includes an opaque substrate that defines the separation channel and the optical slit.3. The method of claim 1 , wherein the fraction of the analyte mixture is expelled via electrospray ionization claim 1 ,4. The method of claim 1 , wherein the orifice is disposed within a recess defined by the microfluidic device claim 1 , such that a Taylor cone formed by electrospray ionization is disposed entirely within the recess.5. The method of claim 1 , wherein monitoring separation of the analytes includes imaging the entire separation channel.6. The method of claim 1 , wherein:the microfluidic device is illuminated with ultraviolet light;the microfluidic device includes a substrate constructed of a cyclic olefin copolymer opaque to ultraviolet light; andthe detected light is only ultraviolet light that transits the separation channel.7. The method of claim 1 , wherein the separation channel is a first separation channel claim 1 , the method further comprising:enriching a fraction of the analyte mixture in a second separation channel of the microfluidic device.8. The method of claim 1 , wherein: ...

MULTI-CHAMBER TISSUE SAMPLE CUP FOR BIOPSY DEVICE

A biopsy device includes a body, a needle, a cutter, and a tissue sample holder. The needle extends distally from the body. The cutter is longitudinally translatable relative to the needle and defines a cutter lumen. The tissue sample holder includes a rotatable member, an individual sample tray, and one or more bulk sample trays. The rotatable member defines a single chamber partially divided by a plurality of tray protrusions extending radially inwardly from a cylindrical wall of the rotatable member. The individual sample tray includes a single sample chamber that is configured to receive a single tissue sample. The bulk sample tray is configured to receive a plurality of tissue samples. 1. A biopsy device comprising:(a) a body;(b) a needle extending distally from the body;(c) a cutter longitudinally translatable relative to the needle, wherein the cutter defines a cutter lumen; and (i) a rotatable member, wherein at least a portion of the rotatable member defines an inner chamber partially divided by a plurality of tray protrusions extending radially inwardly from a cylindrical wall of the rotatable member,', '(ii) an individual sample tray including a single sample chamber, wherein the single sample chamber is configured to receive only a single tissue sample, and', '(iii) one or more bulk sample trays, wherein the bulk sample tray is configured to receive a plurality of tissue samples., '(d) a tissue sample holder including,'}2. The biopsy device of claim 1 , wherein the rotatable member includes a plurality of tray walls that define a plurality of discrete tray chambers within the inner chamber defined by at least a portion of the rotatable member.3. The biopsy device of claim 2 , wherein the plurality of discrete tray chambers includes an individual tray chamber and a bulk tray chamber claim 2 , wherein the individual tray chamber is configured to receive the individual sample tray claim 2 , wherein the bulk tray chamber is configured to receive a single ...

Multichannel air displacement pipettor

A pipetting system is disclosed that includes a pipette head including a multichannel air displacement system for aspirating and dispensing liquids, a pipetting system base that includes a linear guide system along which the pipette head may be moved vertically with respect to the pipetting system base, and counterbalance means for counterbalancing at least a portion of a weight of the pipette head.

Cell capture in microfluidic devices

A capturing of target cells from a biological sample is achieved by inducing a flow of the biological sample in a flow channel (30, 60) of an upstream microfluidic device (1). Target cells present in the biological sample are captured in cell channels (20) of the upstream microfluidic device(1). Once at least a minimum number of target cells are captured in the cell channels (20), the flow of the biological sample in the flow channel is reduced and are verse flow is applied at the upstream microfluidic device (1) to release the target cells captured in the cell channels (20) of the upstream microfluidic device (1) and enable transfer the target cells into cell channels (120) of a downstream microfluidic device (100).

COMPACT MICROFLUIDIC STRUCTURES FOR MANIPULATING FLUIDS

Disclosed is a method and apparatus for manipulating fluids. The apparatus may include a microfluidic structure including inlet channels ( and ) and outlet channels (, and ) oriented among bifurcated (), trifurcated () and merging junctions ( and ). The apparatus splits and merges fluids flowing in the channels to produce successive dilutions of the fluids within the outlet channels. Multiple apparatus may be combined in serial, parallel, combined serial and parallel and/or stacked configurations. One or more apparatus may be used alone or to provide various devices or chambers with the diluted fluids. 1. A method of mixing fluids , comprising:introducing a first fluid into a first inlet channel;introducing a second fluid into a second inlet channel;splitting the first fluid and the second fluid into two channels through a bifurcated junction;merging a first channel of the first fluid with a first channel of the second fluid, thereby forming a mixture of the first and second fluids;splitting the first fluid and the second fluid into a plurality of additional channels through a plurality of bifurcated and trifurcated junctions;merging the first fluid, the second fluid and mixtures thereof into a plurality of additional channels through a plurality of mixing junctions.2. The method of further comprising:causing the first fluid, the second fluid, and mixtures thereof to flow into a gradient chamber.3. The method of claim 2 , further comprising:causing the first fluid, the second fluid, and mixtures thereof to flow into a gradient chamber in a spatial order of decreasing concentration of the first fluid and increasing concentration of the second fluid.4. The method of claim 3 , further comprising:causing the first fluid, the second fluid, and mixtures thereof to flow into a gradient chamber in a spatial order of substantially linearly decreasing concentration of the first fluid and increasing concentration of the second fluid.5. The method of claim 3 , further ...

Tools and methods for isolation and analysis of individual components from a biological sample

The present invention describes a device(s) and assay(s) for the isolation and analysis of individual components from a sample. The invention provides a means of both isolating a multitude of individual components into an organized array and the subsequent analysis of such components by various detection and analysis methodologies. The invention provides a significant advancement in both the number of individual components that can be individually analyzed as well as enabling the quality and number of analytical methodologies that can be applied to them.

MANIPULATION OF FLUIDS, FLUID COMPONENTS AND REACTIONS IN MICROFLUIDIC SYSTEMS

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply. 120-. (canceled)21. A method for partitioning a fluid sample , the method comprising:providing a microfluidic device having a substrate comprising a plurality of microwells in fluid communication with an inlet;introducing a first fluid into the inlet of the microfluidic device, the first fluid comprising a biological sample and a plurality of beads, each bead comprising a reactive component for binding a target molecule from the biological sample to the bead; a subvolume of the first fluid,', 'a portion of the biological sample, and', 'more than one bead; and, 'introducing a second fluid immiscible with the first fluid into the microfluidic device and flowing the second fluid towards each of the plurality of microwells so as to form partitions of fluid in corresponding microwells, wherein at least some of the partitions include'}maintaining each partition of fluid in corresponding microwells for detection of contents in each partition of fluid.22. The method of claim 21 , wherein a subset of the partitions do not include any beads and a second subset of the partitions each include a single bead.23. The method of claim 21 , wherein each partition of fluid is separated from the others by the second fluid.24. The method of claim 21 , wherein the second fluid is an oil.25. The method of claim 21 , further ...

QUALITY/PROCESS CONTROL OF A LATERAL FLOW ASSAY DEVICE BASED ON FLOW MONITORING

A method for providing quality control on a lateral flow assay device or for triggering a process-related step, the device including a substrate having at least one sample receiving area, at least one reagent zone downstream and in fluid communication with the at least one sample receiving area, at least one detection zone downstream and in fluid communication with the at least one reagent zone and at least one wicking zone downstream of the at least one detection zone, each fluidly interconnected therewith along at least one fluid flow path. The detection material provided in the at least one reagent zone produces a detectable signal that can be tracked and monitored prior to the completion of at least one test being performed on the lateral flow assay device. 1. A lateral flow assay device comprising:a substrate;at least one sample addition zone;at least one detection zone downstream and fluidly connected with the at least one sample addition zone, the at least one detection zone being disposed along a linear detection path that enables a detection instrument to determine the presence of at least one analyte of interest in the at least one detection zone;a wicking zone downstream of the at least one detection zone, each of the zones being fluidly interconnected to form a flow path in which sample flows under capillary action from the at least one sample receiving zone to the wicking zone and in which sample is combined with a reagent, the reagent including at least one detection material that produces a detectable signal; andat least one capillary channel for diverting a portion of sample, the at least one capillary channel extending from a portion of the flow path and further extending through the linear detection path of the assay device to permit in situ detection thereof.2. A device as recited in claim 1 , including at least one reagent zone disposed downstream of the at least one sample addition zone claim 1 , the at least one reagent zone retaining said at ...

Chiral separation and analysis by molecular propeller effect

A method and a device for separation and analysis of chiral molecules are described. The method relies on using a fluid shear to induce molecular rotation and the molecular propeller effect to transform rotational motion into translation motion of opposite direction for counterpart enantiomers. The direction of motion of each enantiomers is used to determine its absolute configuration by comparing the direction value with theoretically calculated one. The device uses multiple moving surfaces or pressure induced flows to induce shear flow condition in the solution to separate enantiomers.

Bead incubation and washing on a droplet actuator

Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.

METHODS FOR ASSAYING CELLULAR BINDING INTERACTIONS

There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule. 1. A method of assaying for a binding interaction between a protein produced by a cell and a biomolecule:(a) retaining the cell within a chamber having an inlet and an outlet;(b) exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell;(c) flowing a first fluid volume comprising the biomolecule through the inlet into the chamber and out the outlet, wherein the first fluid volume is in fluid communication with the capture substrate; and(d) determining binding interactions between the protein produced by a cell and the biomolecule.2. The method of claim 1 , wherein the cell is an antibody producing cell (APC) claim 1 , the protein produced by the cell is an antibody and the biomolecule is an antigen.3. The method of or claim 1 , wherein the biomolecule is a fluorescently labeled antigen.4. The method of claim 1 , wherein the determining binding interactions is a measure of antigen-antibody binding kinetics.5. The method of claim 4 , wherein the measure of antigen-antibody binding kinetics is any one or both of: a Krate; and a Krate.6 ...

Wafer level sequencing flow cell fabrication

A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.

Wash through pipettor

A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.

MICROFLUIDIC DEVICES FOR THE RAPID AND AUTOMATED PROCESSING OF SAMPLE POPULATIONS

Microfluidic devices for the rapid and automated processing of sample populations are provided. Described are multiplexer tiplexer microfluidic devices configured to serially deliver a plurality of distinct sample populations to a sample processing element rapidly and automatically, without cross-contaminating the distinct sample populations. Also provided are microfluidic sample processing elements that can be used to rapidly and automatically manipulate and/or interrogate members of a sample population. The microfluidic devices can be used to improve the throughput and quality of experiments involving model organisms, such as

MULTI-STAGE, MULTIPLEXED TARGET ISOLATION AND PROCESSING FROM HETEROGENEOUS POPULATIONS

A system and method for isolating target substrates includes a microfluidic chip, comprising a plurality of processing units, each processing unit comprising: an inlet port, a plurality of first chambers connected to the inlet port by a fluid channel, the fluid channel comprising a plurality of valves, a plurality of second chambers, each of the second chambers connected to a respective first chamber by a fluid channel, each fluid channel including a controllable blocking valve, and a plurality of respective outlet ports, each outlet port in fluid communication with a respective one of said second chambers and each outlet port including a blocking valve. A magnet is adjacent the microfluidic chip and is movable relative to the microfluidic chip. A valve control is capable of actuating certain ones of the controllable blocking valves in response to a control signal. 161-. (canceled)62. A substrate-isolation platform , comprising:a microfluidic chip, comprising a plurality of processing units, each processing unit comprising: an inlet port, a plurality of first chambers connected to the inlet port by a fluid channel, the fluid channel comprising a plurality of valves, a plurality of second chambers, each of the second chambers connected to a respective first chamber by a fluid channel, each fluid channel including a controllable blocking valve, and a plurality of respective outlet ports, each outlet port in fluid communication with a respective one of said second chambers and each outlet port including a blocking valve;a magnet adjacent the microfluidic chip, wherein relative positioning of the magnet and the microfluidic chip is variable; anda valve control capable of actuating certain ones of the controllable blocking valves in response to a control signal.63. The platform of claim 62 , wherein at least one of the first chambers and the second chambers are ring chambers.64. The platform of claim 62 , wherein the microfluidic chip comprises a volume of about 10 nl to ...

Sieve Valves, Microfluidic Circuits, Microfluidic Devices, Kits, and Methods for Isolating an Analyte

The invention generally provides a sieve valve including: a substrate defining a channel; a flexible membrane adapted and configured for deployment at an intersection with the channel; and one or more protrusions extending into the channel from the substrate or the flexible membrane. The one or more protrusions define a plurality of recesses extending beyond the intersection between the channel and the flexible membrane; 1. A method of isolating an analyte , the method comprising:loading a sample into a holding chamber of a microfluidic circuit, the microfluidic circuit including one or more sieve valves, wherein the holding chamber is in fluid communication with an input port of a mixing circuit,capturing an analyte on a capture substrate in the mixing circuit;washing the capture substrate to remove uncaptured components; and a substrate defining a channel;', 'a flexible membrane adapted and configured for deployment at an intersection with the channel; and', 'one or more protrusions extending into the channel from the substrate or the flexible membrane, the one or more protrusions defining a plurality of recesses extending beyond the intersection between the channel and the flexible membrane; and, 'releasing the analyte from the capture substrate, wherein the sieve valve comprises'}passing the sample into the mixing circuit.2. The method of claim 1 , wherein the capture substrate is loaded into the microfluidic circuit claim 1 , the holding chamber claim 1 , and/or the mixing circuit.3. The method of claim 1 , wherein the capture substrate comprises a bead claim 1 , microbead claim 1 , surface of the microfluidic circuit claim 1 , or a capture reagent.4. The method of claim 1 , wherein the mixing circuit comprises a plurality of chambers in fluid communication with one or more holding chambers claim 1 , wherein the one or more holding chambers and/or the mixing circuit comprises the capture substrate for isolating the analyte.5. The method of claim 4 , wherein the ...

Device and method for unit use sensor testing

An analyte testing material web, method of making the analyte testing material web, and an analyzer are disclosed. The analyte testing material web has a material web and a plurality of distinct sample testing devices. The material web has a first surface, a second surface opposite the first surface, a first side, and a second side. The plurality of sample testing devices are positioned on the first surface of the material web. Each of the plurality of sample testing devices has an inlet, an outlet, a fluid channel, and one or more testing elements within the fluid channel and configured to analyze one or more analyte within a sample applied to the inlet of one of the plurality of sample testing devices.

TARGET SUBSTANCE DETECTION DEVICE AND TARGET SUBSTANCE DETECTION METHOD

To detect a target substance accurately and effectively, a target substance detection device includes: a liquid-sample introducing plate formed from a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and enabling propagating transmitted light of light irradiated from the rear face upward of the surface as propagated light; a rear face light irradiation unit configured to be able to irradiate the liquid-sample introducing plate with light from the rear face; a first magnetic field application unit disposed on the side of the surface of the liquid-sample introducing plate , and configured to apply a magnetic field to move the conjugate in the liquid sample that is introduced on the surface of the liquid-sample introducing plate in the direction away from the liquid-sample introducing plate ; and an optical-signal detection unit disposed on the side of the surface of the liquid-sample introducing plate , and enabling detection of a change in optical signal based on the propagated light between before and after application of the magnetic field by the first magnetic field application unit 1. A target substance detection device comprising:a liquid-sample holding unit including a liquid-sample introducing plate introducing any one of: a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and capable of propagating transmitted light of light irradiated from a rear face or the surface to the face on an opposite of the irradiated face as propagated light; a reflective plate having a surface on which the liquid sample is introduced and capable of propagating reflected light of light irradiated from the surface upward of the surface as propagated light; and an introducing plate having a surface on which the liquid sample ...

TRANSMISSION-LINE-COUPLED MICROFLUIDIC-CHIP TECHNOLOGY FOR ELECTROMAGNETIC SENSING OF BIOMOLECULES AND BIOPARTICLES

A coplanar waveguide transmission line for use in detecting biomolecules and bioparticles is provided that includes a signal conductor disposed on a top surface of the dielectric substrate, a ground conductor disposed on the top surface of the dielectric substrate on each side of the signal conductor, a continuous gap defined between the signal conductor and each of the ground conductors, micro-channels disposed below a top surface of the dielectric substrate, and reservoirs disposed below the top surface of the substrate.

Centripetal microfluidic platform for lal reactive substances testing

A centripetal microfluidic platform comprised of a microfluidics disc and a reader for testing LAL-reactive substances in fluid samples is provided. The microfluidic disc may comprise at least two testing areas wherein each testing area includes a reservoir portion for receiving at least one fluid sample. The disc may comprise a distribution network portion in fluid communication with the reservoir portion. Each distribution network portion may comprise a distribution network of at least four (4) channels, wherein each channel has a metering portion and at least one analysis chamber portion. The analysis chamber portion may comprise a mixing chamber for mixing samples and reagents and an optical chamber portion that is compatible with an optical reader. The metering portion may be sized to meter an aliquot of the fluid sample for analysis in the analysis chamber portion. At least one analysis chamber portion has at least one reagent isolated therein. The centripetal microfluidic platform further includes a reader for testing fluid samples within a microfluidic disc comprising an enclosure, an optical bench, a centripetal disc drive, and a controller. A method for testing at least one fluid sample for LAL-reactive substances is also provided.

ASSAY DEVICES, METHODS FOR CARRYING OUT ASSAYS, ASSAY KITS AND METHOD FOR MANUFACTURING ASSAY DEVICES

The present invention provides assay devices having a unitary body with an exterior surface, the unitary body being substantially transparent to visible light and formed from a material having a refractive index in the range 1.26 to 1.40, the refractive index being measured at 20° C. with light of wavelength 589 nm, and wherein the unitary body is formed from a hydrophobic material, and at least two capillary bores extending internally along the unitary body, wherein at least a portion of the surface of each capillary bore includes a hydrophilic layer for retaining an assay reagent, and wherein the hydrophilic layer is also substantially transparent to visible light to allow optical interrogation of the capillary bores through the capillary wall. The present invention also provides assay systems including such assay devices, methods of performing an assay using such assay devices and method of method for manufacturing such assay devices. 1. An assay device having:a unitary body with an exterior surface, the unitary body being substantially transparent to visible light and formed from a material having a refractive index in the range 1.26 to 1.40, the refractive index being measured at 20° C. with light of wavelength 589 nm, and wherein the unitary body is formed from a hydrophobic material, andat least two capillary bores extending internally along the unitary body, wherein at least a portion of the surface of each capillary bore includes a hydrophilic layer for retaining an assay reagent, andwherein the hydrophilic layer is also substantially transparent to visible light to allow optical interrogation of the capillary bores through the capillary wall.2. The assay device according to claim 1 , wherein the hydrophilic layer is coated onto the surface of one or more capillary bores.3. The assay device according to claim 1 , wherein an assay reagent is retained at least at a portion of the hydrophilic layer of one or more capillary bores.4. The assay device according ...

VERTICAL MICROFLUIDIC PROBE HEAD WITH LARGE SCALE SURFACE PROCESSING APERTURES

One or more embodiments of the present invention are directed to a method for processing a surface with a vertical microfluidic probe head. The method includes positioning the microfluidic probe head so as for the edge surface to face a surface to be processed. Next, the method dispenses processing liquid via each orifice of the first one of the sets of n orifices, so as for the dispensed processing liquid to process the surface; and aspirates liquid via each orifice of the second one of the sets of n orifices. 1. A method for processing a surface with a vertical microfluidic probe head , the method comprising:positioning the microfluidic probe head so as for the edge surface to face a surface to be processed;dispensing processing liquid via each orifice of the first one of the sets of n orifices, so as for the dispensed processing liquid to process the surface; andaspirating liquid via each orifice of the second one of the sets of n orifices.2. The method of claim 1 , wherein aspirating liquid comprises aspirating claim 1 , via an orifice of the second one of the sets of n orifices claim 1 , at least some of the processing liquid dispensed through an orifice of the first one of the sets of n orifices.3. The method of claim 1 , wherein the method further comprises:providing an immersion liquid on the surface to be processed;wherein the microfluidic probe head is positioned so as to subsequently dispense processing liquid within the immersion liquid.4. The method of claim 3 , wherein the two main surfaces of the chip are claim 3 , each claim 3 , multiply grooved up to the edge surface claim 3 , such that n≥2 claim 3 , and wherein the processing liquid is dispensed so as to form laminar flows of processing liquid from each orifice of the first set of orifices.5. The method of claim 1 , wherein the method further comprises moving the microfluidic probe head opposite to the surface to be processed while dispensing processing liquid.6. The method of claim 5 , wherein the ...

Tagged ligands for enrichment of rare analytes from a mixed sample

Method of enriching specific cells from cellular samples are disclosed, comprising contacting in solution a cellular sample with affinity-tagged ligands (ATLs) each comprising a first ligand linked to an affinity tag, wherein the ligand selectively binds a cellular marker of the rare cells and the affinity tag can be selectively captured by a capture moiety, wherein the affinity tags do not comprise a magnetic particle; and flowing the sample through a microfluidic device comprising the capture moiety to selectively retain ATL-bound cells. Methods for enriching circulating tumor cells, and devices for enriching specific cells from cellular samples are also disclosed.

DIAGNOSTIC CHIP

A microfluidic diagnostic chip may comprise a substrate, a plurality of fluidic slots extending through the substrate, a plurality of microfluidic channels each coupled to a respective one of the plurality of fluidic slots to receive a plurality of fluids from the plurality of fluidic slots, and a mixing region in fluid communication with the plurality of fluidic slots to receive the plurality of fluids such that the plurality of fluids are to mix. A diagnostic chip may comprise a number of fluid slots defined through a substrate and a plurality of microfluidic channels coupled to the fluid slots to receive from the fluid slots a plurality of different fluids wherein the microfluidic channels combine and mix the plurality of different fluids. 1. A microfluidic diagnostic chip , comprising:a substrate;a plurality of fluidic slots extending through the substrate;a plurality of microfluidic channels each coupled to a respective one of the plurality of fluidic slots to receive a plurality of fluids from the plurality of fluidic slots; anda mixing region in fluid communication with the plurality of fluidic slots to receive the plurality of fluids such that the plurality of fluids are to mix.2. The microfluidic diagnostic chip of claim 1 , wherein each of the plurality of microfluidic channels comprise at least one micropump.3. The microfluidic diagnostic chip of claim 2 , wherein the micropumps cooperate to cause the plurality of fluids to pass through the respective microfluidic channels to mix the plurality of fluids to produce a specified composition of the plurality of fluids.4. The microfluidic diagnostic chip of claim 3 , wherein a number of the plurality of microfluidic channels comprise a number of sensors to detect properties of the fluids.5. The microfluidic diagnostic chip of claim 4 , wherein the number of sensors are to detect the properties of the fluids before mixing.6. The microfluidic diagnostic chip of claim 4 , wherein the number of sensors are to ...

DEVICE AND METHOD FOR GENERATING DROPLETS

The invention relates to a device () for generating droplets () comprising a plurality of channels (), wherein each channel () extends from an inlet () along a respective longitudinal axis (L) to an outlet (), wherein said device () comprises a plurality of layers () of a substrate material arranged in a stack (), wherein each layer () comprises a first side () and a second side () facing away from each other, and wherein said first side () of each layer () comprises a plurality of grooves (), wherein said channels () are formed by said grooves () of said first side () of a respective layer () of said stack () and said second side () of a respective adjacent layer () of said stack (). The invention further relates to a method for generating droplets () and a fabrication method of the device (). 113020202012022020120230202201202202. A device () for generating droplets () of a dispersed phase (D) in a continuous phase (C) , comprising a plurality of channels () , wherein each channel () comprises an inlet () and an outlet () , and wherein each channel () extends from said inlet () along a respective longitudinal axis (L) to said outlet () , so that droplets () of a dispersed phase (D) can be generated in a continuous phase (C) at said outlets () when a flow of said dispersed phase (D) from said inlets () to said outlets () is provided and said outlets () are in flow connection with a reservoir or conduit containing said continuous phase (C) , characterized in that{'b': 1', '10', '100', '10', '101', '102', '101', '102', '101', '10', '103', '103', '101', '102', '10', '20', '201', '104', '100', '202', '105', '100, 'said device () comprises a plurality of layers () of a substrate material arranged in a stack (), wherein each layer () comprises a first side () and a second side (), wherein the first side () faces away from the second side (), and wherein the first side () of each layer () comprises a plurality of grooves (), wherein the grooves () of each first side () are ...

OPTICAL REACTION WELL FOR ASSAY DEVICE

This disclosure relates to an apparatus for simultaneously filling a plurality of sample chambers. In one aspect, the apparatus comprises a common fluid source and a plurality of independent, continuous fluidic pathways. Each independent, continuous fluidic pathway comprises a sample chamber and a pneumatic compartment. The sample chamber is connected to the common fluid source, and the pneumatic compartment is connected to the sample chamber. The sample chamber comprises, in part, an assay chamber. The assay chamber comprises a monolithic substrate and a plug having optically transmissive properties. In some embodiments, the assay chamber contains a magnetic mixing element. In some embodiments, the assay chamber is a double tapered chamber. In some embodiments, a ratio of a volume of the sample chamber to a volume of the pneumatic compartment is substantially equivalent for each fluidic pathway of the plurality of fluidic pathways. 1. A plug comprising:A body with a bottom surface:A central opening in the body; andA dried reagent on the bottom surface, wherein the body is formed from a material transmissive to excitation wavelengths and emission wavelengths in at least one of a red spectrum, a blue spectrum and a green spectrum.2. The plug of wherein the dried reagent is on a portion of the bottom surface wider than a width of the central opening in the body.3. The plug of wherein a width of the central opening is wider than a portion of the bottom surface containing the dried reagent.4. The plug of further comprising a cavity in the bottom surface with the dried reagent within the cavity.5. The plug of further comprising a plug thickness between a central opening bottom and the plug body bottom wherein a depth of the cavity is less than 90% of the plug thickness.6. The plug of further comprising a plug thickness between a central opening bottom and the plug body bottom wherein a depth of the cavity is less than 70% of the plug thickness.7. The plug of further ...

Sample Supply System and Methods of Supplying Samples

Disclosed are high-throughput vessel supply systems and methods of supplying sample vessels, such as samples stored in test tubes. A system for supplying a plurality of individual vessels that each contains a sample is disclosed. 1. A system for supplying a plurality of vessels , comprising: (i) a feed bin comprising a bottom surface, a substantially open top, a front side, a back side, an A-side, and a B-side, and configured to feed each of the plurality of vessels from an entry position at the top of the feed bin to at least one edge position of the bottom surface wherein the feed;', '(ii) at least one transporter positioned on at least one of the A-side or the B-side of the feed bin and configured to receive each of the plurality of vessels from the at least one edge position and transport each of the vessels to at least one transfer point; and', '(iii) at least one transfer belt positioned at the exit of the at least one transporter and at or near the top of the feed bin, and configured to receive each of the plurality of vessels from at least one transfer point and individually transfer individual vessels from the at least one transfer point to a bin exit position within the system., 'a feeding unit configured to supply a plurality of vessels to at least one exit position, wherein each of the plurality of vessels is configured to hold a sample, and wherein the feeding unit comprises2. The system of claim 1 , wherein the plurality of vessels comprise a biological sample tube that is cylindrical in shape and comprises a cap.3. The system of claim 1 , wherein each of the plurality of vessels ranges from 75 to about 125 mm in length and about 8 to about 16 mm in diameter.4. The system of claim 1 , wherein an operating path of the at least one transfer belt is substantially perpendicular to a direction of advancement of the at least one transporter.5. The system of claim 1 , wherein the feed bin further comprises an interior slope at a convergence of the A-side and ...

MULTICHANNEL PIPETTING SYSTEM COMPRISING TWO ASPIRATION CHAMBERS THAT ARE IMBRICATED IN ONE ANOTHER

The invention relates to a device () for the bottom part of a multichannel pipetting system, comprising two sampling-cone-holding end pieces () and also two aspiration chambers () that engage respectively with the two end pieces, the two aspiration chambers () being concentric. 1. A device forming a bottom part of a multichannel pipetting system , the device comprising;two sampling-cone-holding end pieces as well as two aspiration chambers respectively engaging with said two sampling-cone-holding end pieces,wherein both of said two aspiration chambers are concentric.2. The device according to claim 1 , further comprising two pistons respectively engaging with said two aspiration chambers claim 1 , both of said two pistons being imbricated in one another.3. The device according to claim 2 , wherein both of said two pistons are each translationally secured claim 2 , along their sliding direction in the aspiration chambers.4. The device according to claim 2 , wherein both of said two aspiration chambers are arranged one around the other.5. The device according to claim 2 , wherein a first of said two aspiration chambers has a solid transverse cross-section claim 2 , and a second of said two aspiration chambers has a recessed transverse cross-section.6. The device according to claim 5 , wherein the first aspiration chamber has a substantially disc-shaped transverse cross-section claim 5 , and the second aspiration chamber has a substantially annular shaped transverse cross-section.7. The device according to claim 1 , further cornprising:a first aspiration chamber and a second aspiration chamber, as well as first and second pistons respectively engaging with said first and second aspiration chambers, said second aspiration chamber having a substantially annular shaped transverse cross-section and being defined between an internal body and an external body, said second piston being accommodated in the second chamber which is provided in the form of a sealed ring at one of ...

MICROCHANNEL CHIP, PCR METHOD, AND HEATING/COOLING CONTROL APPARATUS

A method for performing a polymerase chain reaction (PCR) process on a treatment target liquid using a microchannel chip and a heating/cooling control apparatus is provided. The microchannel chip includes: a first substrate layer that has an introducing channel and a discharging channel; and a second substrate layer that is disposed on the first substrate layer and has a second substrate layer body and a metal film, the second substrate layer body having a microchannel connected to the two channels, the metal film structuring an upper surface of the microchannel. The heating/cooling control apparatus includes: a temperature regulator; a power source that supplies voltage to the temperature regulator; and a heating/cooling controller that controls heating or cooling of the temperature regulator by controlling the voltage. The heating/cooling controller controls the voltage to change the temperature of the temperature regulator. The heat of the temperature regulator is conducted to the liquid in the microchannel via the metal film, thereby achieving the PCR process. 1. A polymerase chain reaction (PCR) method for performing a PCR process on a treatment target liquid using a microchannel apparatus including a microchannel chip and a heating/cooling control apparatus , the PCR method comprising: the microchannel chip including:', 'a first substrate layer comprising an introducing channel and a discharging channel; and', 'a second substrate layer that is disposed on the first substrate layer and comprises a second substrate layer body and a metal film, the second substrate layer body comprising a microchannel connected to the introducing channel and the discharging channel, the microchannel being filled with the treatment target liquid, the metal film structuring an upper surface of the microchannel,', 'the heating/cooling control apparatus including:', 'a temperature regulator that is disposed so as to be in contact with the metal film of the second substrate layer and ...

Device for standard preparation of urinary sediment for urine analysis (Mono System)

A System for use in sample processing for urine sediment analysis that is composed of a main tube and a thinner inner tube (without capillarity), which is attached to the wall of the main tube and has the volume equal to one twelfth of the main tube. Near an upper end of both tubes, a small connected aperture on each tube allows evacuation of air from the inner tube when urine enters this tube from an open end. After urine centrifugation, the small opening is closed by operator's finger and the supernatant is poured out from the main tube. This allows retention of the urine in the inner tube, which will be added to the sediment after unblocking of the aperture. 1. A Mono System for urine sediment analysis comprising: a main tube comprising a closed distal end and an open proximal end , having a specific volume; an inner tube , located inside said main tube and comprises a connecting point with said main tube; wherein said inner tube comprises an open proximal end and an open distal end , where said open distal end is located near said closed distal end of said main tube; and wherein said main tube and said inner tube are parallel through their respective axles and where said inner tube does not have capillarity properties and is very thin in diameter in comparison to said main tube , wherein said inner tube comprises a small aperture at said connecting point; wherein said aperture allows outside air to flow directly inside said inner tube; wherein said inner tube comprises a maximum volume of 1/12of said volume of said main tube; wherein when a fluid sample is poured inside said main tube said proximal open end of said inner tube is blocked from outside air via an index finger and therefore 1/12of said fluid sample will be trapped/retained inside said inner tube; then remaining fluid inside said main tube is discarded by tilting said main tube while said index finger is still blocking said proximal end of said inner tube , when said fluid is completely discarded ...

METHODS AND APPARATUS TO AGITATE A LIQUID

Method and apparatus to agitate a liquid are disclosed herein. An example apparatus includes a carrier having a base that includes a ridge extending from the base and a collar extending from the base. The example apparatus also includes a container supported on the base, the container movable between (A) a locked positon in which the ridge fixedly engages the container to non-rotatably couple the container to the base and (B) an unlocked position in which the container is disengaged from the ridge and the container is rotatable about the collar. 1. A method comprising:lifting a container, coupled at a top and a bottom of the container to a carrier, from a first position in which the container is rotatably coupled to the carrier and a second position in which the container is non-rotatably coupled to the carrier; androtating the container, when the container is the second position, about an axis of rotation to mix contents of the container.2. The method of claim 1 , wherein the container is a first container claim 1 , further including non-rotatably supporting a second container on the carrier while rotating the first container.3. The method of claim 1 , further including lowering the container to the first position to non-rotatably couple the container to the carrier.4. The method of claim 1 , further including removing a cap from the container to enable the container to move from the first position to the second position.5. The method of claim 1 , wherein the axis of rotation is a first axis of rotation claim 1 , further including rotating the carrier on a carousel about a second axis of rotation while the container is rotating about the first axis of rotation.6. The method of claim 1 , wherein lifting the container includes engaging a shaft with the bottom of the container.7. The method of claim 6 , wherein a base of the carrier includes an opening claim 6 , and wherein lifting the container includes inserting the shaft through the opening to engage the bottom of ...

Separation device for use in the separation, characterization and/or identification of microorganisms

The present invention is directed to a separation device or container that can be used in the separation, isolation or pelleting of microorganisms from a test samples known to contain or suspected of containing said microorganisms. Subsequently, the separated, isolated or pelleted microorganism sample can undergo one or more interrogation steps to provide measurements useful for the characterization and/or identification of microorganism. In one aspect of the present invention, the interrogation steps can occur in situ in the separation device or container described herein.

SYSTEMS, DEVICES AND METHODS FOR MICROFLUIDIC ANALYSIS

Described herein are various inventions and embodiments thereof, directed to systems, devices, and methods for analysis of a biofluid, as well as controlling a biofluid analysis system using a microfluidic device. Embodiments of biofluid analysis systems disclosed herein may provide analysis of a biofluid to identify and characterize one or more analytes. An apparatus may include a first layer defining a first opening and a second opening. The first layer may be substantially transparent. A second layer may be coupled to the first layer and define a microfluidic channel that establishes a fluid communication path between the first opening and the second opening. At least a portion of the second layer may be substantially opaque. 1. An apparatus , comprising:a first layer defining a first opening and a second opening, the first layer being substantially transparent; anda second layer coupled to the first layer and defining a microfluidic channel that establishes a fluid communication path between the first opening and the second opening, at least a portion of the second layer being substantially opaque.2. The apparatus of claim 1 , wherein the first layer is substantially transparent to at least one of ultraviolet light claim 1 , visible light claim 1 , and near-infrared light.3. The apparatus of claim 1 , wherein the microfluidic channel is linear relative to a longitudinal axis of the apparatus.4. The apparatus of claim 1 , wherein the microfluidic channel is curved relative to a longitudinal axis of the apparatus.5. The apparatus of claim 1 , wherein the microfluidic channel is parallel and offset from a central longitudinal plane of the apparatus.6. The apparatus of claim 1 , wherein the microfluidic channel is defined along a central longitudinal plane of the apparatus.7. The apparatus of claim 1 , wherein a height of the microfluidic channel decreases continuously from the first opening to the second opening.8. The apparatus of claim 1 , wherein a side of the ...

DISSOLVING DROPLET MICROFLUIDIC PARTICLE ASSEMBLY DEVICES AND METHODS, AND PARTICLE ASSEMBLIES OBTAINED THEREFROM

Some variations provide a device for assembling a plurality of particles into particle assemblies, comprising: (a) a microfluidic droplet-generating region; (b) a first inlet to the droplet-generating region, configured to feed a first fluid containing particles and a solvent for the particles; (c) a second inlet to the droplet-generating region, configured to feed a second fluid that is not fully miscible with the first fluid; (d) a droplet outlet from the droplet-generating region, configured to withdraw droplets of the first fluid dispersed in the second fluid; and (e) a droplet-dissolving region configured to remove solvent from the droplets, thereby forming particle assemblies. Some variations also provide an assembly of nanoparticles, wherein the assembly has a volume from 1 μmto 1 mm, a packing fraction from 20% to 100%, and/or an average relative surface roughness less than 1%, wherein the assembly is not disposed on a substrate. 1. A device for assembling a plurality of particles into particle assemblies , said device comprising:(a) a microfluidic droplet-generating region;(b) a first inlet to said droplet-generating region, wherein said first inlet is configured to feed a first fluid containing particles and a solvent for said particles;(c) a second inlet to said droplet-generating region, wherein said second inlet is configured to feed a second fluid that is not fully miscible with said first fluid;(d) a droplet outlet from said droplet-generating region, wherein said droplet outlet is configured to withdraw droplets of said first fluid dispersed in said second fluid; and(e) a droplet-dissolving region configured to receive said droplets from said droplet outlet, wherein said droplet-dissolving region is configured to remove said solvent from said droplets, thereby forming particle assemblies.2. The device of claim 1 , said device further comprising a third inlet to said droplet-generating region claim 1 , wherein said third inlet is configured to feed ...

BLOOD VESSEL-MIMICKING MICROFLUIDIC CHIP FOR CELL CO-CULTURE AND USE THEREOF

The present disclosure provides a blood vessel-mimicking microfluidic chip for cell co-culture and a use thereof, wherein the microfluidic chip of the present disclosure is a microfluidic chip capable of co-culturing vascular endothelial cells and cancer cells, and can mimic normal vascular tissue, cancer tissue, and cancer-metastatic vascular tissue, and therefore can be widely used in studies associated with cancer, and especially, is suitable in studies on cancer metastasis, intravenous injection environments for cancer treatment, photothermal therapeutic effects on cancer cell, and the like. 1. A blood vessel-mimicking microfluidic chip for cell co-culture , the microfluidic chip comprising:(a) a first cell culture channel, a second cell culture channel, and a cell co-culture channel, as cell culture sections; and(b) bridge channels connected to the cell culture channels,wherein the cell co-culture channel is disposed between the first cell culture channel and the second cell culture channel and the first cell culture channel, the second culture channel, and the cell co-culture channel are connected through hollow tubular bridge channels.2. The microfluidic chip of claim 1 , wherein different types of cells selected from the group consisting of cancer cells and vascular endothelial cells are cultured in the first cell culture channel and the second cell culture channel claim 1 , respectively.3. The microfluidic chip of claim 1 , wherein cancer cells and vascular endothelial cells are co-cultured in the cell co-culture channel.4. The microfluidic chip of claim 1 , wherein the microfluidic chip is manufactured of a polymer material selected from the group consisting of poly(dimethylsiloxane) (PDMS) claim 1 , polymethylmethacrylate (PMMA) claim 1 , polyacrylates claim 1 , polycarbonates claim 1 , polycyclic olefins claim 1 , polyimides claim 1 , and polyurethanes.5. The microfluidic chip of claim 1 , wherein the microfluidic chip is bonded onto a plate facilitating ...

METHODS FOR ASSAYING CELLULAR BINDING INTERACTIONS

There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule. 145.-. (canceled)46. A method of assaying for a binding interaction between an antibody secreted by a single antibody secreting cell (ASC) and an antigen , the method comprising:retaining the single ASC within a chamber having a volume of from 100 μL to 100 nL, a solid wall, and an aperture that defines an opening of the chamber;incubating the single ASC within the chamber to produce a secreted antibody;bringing a first fluid volume comprising the antigen in fluid communication with the secreted antibody;exposing the secreted antibody to a removeable capture substrate, wherein the removeable capture substrate is in fluid communication with the secreted antibody and wherein the removeable capture substrate is operable to bind the secreted antibody;incubating the secreted antibody with the removeable capture substrate to produce a bound antibody; andmeasuring a binding interaction between the secreted antibody and the antigen.47. The method of claim 46 , wherein the single APC is a single primary plasma cell.48. The method of claim 46 , wherein the single APC is an activated B cell.49. The method of claim 46 , wherein the antigen is a cell or a cell fragment.50. The method of claim 46 , wherein the antigen is a virus or ...

Methods for High-Content Drug Screening

Provided herein are methods and systems for screening a candidate agent to determine whether the candidate agent modulates an activity of cultured cells. Compositions for screening a candidate agent are also provided herein. 1. A method of screening a candidate agent to determine whether the candidate agent modulates an activity of cultured cells , the method comprising:positioning a hydrogel comprising at least one candidate agent in a lumen of a hollow micropillar, wherein the hollow micropillar comprises a first surface having an open end and a second surface having a closed end in contact with a first substrate, wherein the hollow micropillar is orthogonal to the first substrate;bringing the first surface of the hollow micropillar into communication with a surface of cultured cells on a second substrate to provide an interaction gap between the first surface of the hollow micropillar and the surface of the cultured cells, wherein the first surface of the hollow micropillar is in a spaced apart and in facing relationship with the surface of cultured cells;adding a solution to the interaction gap such that the at least one candidate agent is released from the hydrogel onto the surface of cultured cells; andmeasuring a signal from the cultured cells, wherein the signal indicates whether the at least one candidate agent modulates the activity of the cultured cells.2. The method of claim 1 , wherein the hydrogel comprises alginate.3. The method of or claim 1 , wherein the at least one candidate agent comprises at least two candidate agents.4. The method of or claim 1 , wherein the at least one candidate agent comprises at least one thousand candidate agents.5. The method of any one of - claim 1 , wherein the positioning comprises filling the lumen of the hollow micropillar such that a surface of the hydrogel in the lumen is coplanar with the first surface of the hollow micropillar.6. The method of any one of - claim 1 , wherein the positioning comprises filling a ...

Device, System And Method For Processing A Sample

A device for the processing of a sample comprises a location apparatus, a processing chamber for receiving the sample and a plurality of reagent chambers. The reagent chambers have openings defined in the location apparatus. The processing chamber is movable relative to the reagent chambers to enable sequential communication with each reagent chamber in turn.

Nanofluidic Device for Isolating, Growing, and Characterizing Microbial Cells

Nanofluidic devices and methods of the invention are capable of autonomously isolating individual microbial cells using constrictive channels and growing monocultures of the cells for automated characterization of their biochemical properties and interactions with mammalian cells. Single microbial cells, such as bacterial cells, are isolated directly from environmental sources and cultured using chemical factors from their native environment. 1. A method of isolating and culturing a single microbial cell to obtain a monoculture of microbial cells , the method comprising the steps of:(a) exposing a fluid sample containing a mixture of microbial cells to a device comprising a food chamber in fluid communication with an opening in the device, wherein the food chamber contains a culture medium containing a chemoattractant for at least one microbial cell in the sample and capable of supporting the growth and reproduction of said microbial cell, and wherein the opening allows only partial entry of a single microbial cell;(b) allowing said microbial cell to migrate into the opening of the device;(c) maintaining the device under conditions suitable for allowing said microbial cell to divide while residing in the opening and produce progeny, whereby the progeny eventually enter the food chamber;(d) maintaining the device under conditions suitable for the progeny entering the food chamber to multiply in the food chamber, forming a monoculture of microbial cells.2. The method of claim 1 , wherein the device comprises a plurality of microfluidic food chambers claim 1 , each fluidically coupled to a separate opening in the device; and wherein a plurality of monocultures are obtained claim 1 , each grown in a distinct food chamber and derived from a distinct single microbial cell of the sample.3. The method of claim 2 , wherein the device comprises 100 or more food chambers claim 2 , each fluidically coupled to a separate opening in the device.4. The method of claim 2 , whereby ...

DIGITAL PROTEIN SENSING CHIP AND METHODS FOR DETECTION OF LOW CONCENTRATIONS OF MOLECULES

A sensing device is provided that includes a tunnel junction created by forming a hole in a layered tunnel junction (for example). A chemically, well-defined surface may be formed by coupling affinity reagents to the electrodes, which, by these means, the surface may be configured to be selective for a particular analyte. 1. A device for detecting the binding of a molecule to a cognate ligand , comprising:a first planar electrode having a length, a width and a thickness;an insulating layer having a length, a width and a thickness, the insulating layer covering a substantial portion of the first electrode;a second planar electrode having a length, a width and a thickness, and arranged adjacent the insulating layer, wherein the insulating layer is sandwiched between the first and second electrodes, the second electrode configured with a width that is less than the width of the first electrode;an opening through the electrodes configured to expose the gap between them and establishing an electrodes-insulating layer junction, wherein the opening is spaced away from the perimeter of each electrode;one or more recognition molecules comprising a cognate ligand;a chemical group configured to couple the cognate ligand to at least one of the electrodes; anda reference electrode in communication with at least one of the electrodes.2. The device of claim 1 , wherein the reference electrode is configured with a substantially constant potential difference with respect to at least one of the electrodes.3. The device of claim 1 , wherein the cognate ligand comprises at least one of an antibody claim 1 , a Fab fragment claim 1 , an aptamer and a peptide configured to bind to one or more protein targets.4. The device of claim 1 , wherein the device is configured to detect single molecular binding events corresponding to low concentration sample solutions.5. The device of claim 1 , wherein the device is configured for sequencing a peptide chain.6. A system for detecting the binding of ...

Self-Contained Nucleic Acid Processing

Instruments and methods for amplifying nucleic acids in a sample provided in a flexible, self-contained, substantially closed sample container. 1. An instrument for processing a sample , comprisinga first support member and a second support member; a loading attachment,', 'a plurality of reaction zones, and', 'one or more sealable flow paths, each having a first side connecting the loading attachment to one or more fill ports, at least one of which is configured for receiving the sample, and a second side connected to at least one of the plurality of reaction zones, the sealable flow paths providing the only access from the one or more fill ports to the plurality of reagent zones such that when the first side of all of the one or more sealable paths are sealed, the container is fully closed;, 'an opening for receiving a flexible reaction container between the first support member and the second support member, the container comprisinga plurality of heat seal elements provided on at least one of the support members so as to contact the loading attachment and the one or more sealable flow paths, wherein the heat seal elements include a number of individually controlled heater elements associated with each of the first side of the one or more sealable flow paths.2. The instrument of claim 1 , wherein the individually controlled heater elements are ceramic heater elements.3. The instrument of claim 2 , wherein the ceramic heater elements are self-regulating heater elements.4. The instrument of claim 3 , wherein the self-regulating heater elements include positive thermal coefficient (PTC) heating elements.5. The instrument of claim 3 , wherein the PTC heating elements are self-limiting.6. The instrument of claim 1 , wherein the individually controlled heater elements are selected from the group consisting of resistance wire heating elements claim 1 , ceramic heating elements claim 1 , etched foil heating elements claim 1 , and combinations thereof.7. The instrument of ...

PRECISION FABRICATION OF NANOSIEVES

An exemplary method includes forming a sacrificial layer along sidewalls of an array of trenches that are indented into a substrate, depositing a fill layer over the sacrificial layer, and then creating an array of gaps between the fill layer and the substrate by removing the sacrificial layer along the sidewalls of the trenches, while maintaining a structural connection between the substrate and the fill layer at the floors of the trenches. The method further includes covering the substrate, the fill layer, and the gaps with a cap layer that seal fluid-tight against the substrate and the fill layer. The method further includes indenting a first reservoir and a second reservoir through the cap layer, and into the substrate and the fill layer, across the lengths of the array of gaps, so that the array of gaps connects the first reservoir in fluid communication with the second reservoir. 1. An apparatus comprising:a substrate;an array of first trenches formed in the substrate;a fill layer deposited into the array of first trenches, with an array of gaps between the fill layer and side walls of the array of first trenches;first and second reservoirs indented through the fill layer and into the substrate across the array of first trenches, wherein the array of gaps connect the first reservoir in fluid communication with the second reservoir; anda cap layer deposited over the substrate and the fill layer to fluidly seal the array of gaps.2. The apparatus of wherein the sacrificial layer is formed with thickness variation less than 5 nm.3. The apparatus of wherein the sacrificial layer is formed with thickness variation less than 5 atoms.4. An apparatus comprising:a substrate;an array of first trenches formed in the substrate;a fill layer deposited into the array of first trenches, with an array of gaps between the fill layer and side walls of the array of first trenches;first and second reservoirs indented through the fill layer and into the substrate across the array of ...

MANIPULATION OF FLUIDS, FLUID COMPONENTS AND REACTIONS IN MICROFLUIDIC SYSTEMS

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply. 179.-. (canceled)80. A method for partitioning a fluid sample , the method comprising:providing a microfluidic device having a substrate comprising a plurality of microwells in fluid communication with an inlet;introducing a first fluid into the inlet of the microfluidic device, the first fluid comprising a biological sample and a plurality of beads, each bead comprising a reactive component for binding a target molecule from the biological sample to the bead;introducing a second fluid immiscible with the first fluid into the microfluidic device and flowing the second fluid towards each of the plurality of microwells so as to form partitions of fluid in corresponding microwells, each partition of fluid comprising a subvolume of the first fluid, including at least the biological sample and a single bead, wherein each partition of fluid is separated from one another by the second fluid; andmaintaining each partition of fluid in corresponding microwells for detection of contents in each partition of fluid.81. The method of claim 80 , wherein each partition of fluid is at least partially surrounded by the second fluid.82. The method of claim 80 , wherein the second fluid is an oil.83. The method of claim 80 , further comprising monitoring each partition of fluid and detecting claim 80 , with a detector claim 80 ...

MICROREACTOR CHIP AND MANUFACTURING METHOD FOR SAME

A microreactor chip includes a substrate and a hydrophobic layer that is a layer provided on the substrate and made of a hydrophobic substance and is formed so that openings of a plurality of chambers are arranged regularly on a main surface of the layer. Each chamber is provided with a first lipid bilayer membrane and a second lipid bilayer membrane that are disposed with a gap therebetween in a depth direction so as to fractionate the chamber in the depth direction. 1. A microreactor chip comprising:a substrate; anda hydrophobic layer that is a layer provided on the substrate and made of a hydrophobic substance and is formed so that openings of a plurality of chambers are arranged regularly on a main surface of the layer, whereineach chamber is provided with a first lipid bilayer membrane and a second lipid bilayer membrane that are disposed with a gap therebetween in a depth direction so as to fractionate the chamber in the depth direction.2. The microreactor chip according to claim 1 , wherein{'sup': −18', '3, 'a capacity of each chamber is 4000×10mor less.'}3. The microreactor chip according to claim 1 , whereinan interval between the first lipid bilayer membrane and the second lipid bilayer membrane is 10 μm or less.4. The microreactor chip according to claim 1 , whereinat least one of the first lipid bilayer membrane and the second lipid bilayer membrane holds a membrane protein.5. The microreactor chip according to claim 1 , whereineach chamber is provided with a third lipid bilayer membrane that is disposed with a gap in the depth direction with respect to the first lipid bilayer membrane and the second lipid bilayer membrane so as to further fractionate the chamber in the depth direction.6. A method for manufacturing a microreactor chip claim 1 , the method comprising:a step of preparing the microreactor chip before lipid bilayer membrane formation, the microreactor chip including a substrate and a hydrophobic layer that is a layer provided on the ...

IMPROVEMENTS IN AND RELATING TO MAGAZINES FOR HOLDING PLURAL FLAT CARDS

Disclosed is a lidded magazine () for holding plural generally flat sample collection cards () in spaced parallel relation, the magazine is open at its top and includes upstanding opposed side walls (), each wall () further including plural opposed pairs of inwardly extending wall ribs () spaced along each wall () to provide therebetween card accepting slots, a first slot dimension (W) being defined by the spacing between an interior face () of each wall, the magazine being characterised in that the walls () at the top () of each slot () are tapered outwardly such that the first dimension (W) is greatest at the top of the slot (). 110010110120110120112122110120154110120152150150. A magazine () for holding plural generally flat sample collection cards () in spaced parallel relation at their edges only , the magazine open at its top and including upstanding opposed side walls ( ,) , each wall ( ,) further including plural opposed pairs of inwardly extending wall ribs ( ,) spaced along each wall ( ,) to provide therebetween card accepting slots , a first slot dimension (W) being defined by the spacing between an interior face () of each wall , the magazine being characterised in that the walls ( ,) at the top () of each slot () are tapered outwardly such that the first dimension (W) is greatest at the top of the slot ().2152. A magazine as claimed in claim 1 , wherein the side walls have a thickness (D) which tapers toward the top () of the walls claim 1 , to provide said enlarged first dimension (W).3112122130110120134132150. A magazine as claimed in or claim 1 , wherein the wall ribs ( claim 1 ,) have a cross piece () extending generally in the same direction as a respective wall ( claim 1 ,) claim 1 , wherein claim 1 , adjacent ends () of neighbouring cross pieces are spaced to define a gap which gap defines a second slot dimension (T) said cross pieces having a narrowing upper end () such that the second dimension (T) is enlarged at the top of the slot ().4. A ...

MODULAR FLUIDIC CHIP AND FLUIDIC FLOW SYSTEM COMPRISING SAME

A modular fluidic chip according to an embodiment of the present disclosure includes a body configured to have at least one flow channel formed in an inside thereof and be connected to another modular fluidic chip to allow the at least one flow channel to communicate with a flow channel provided in the other modular fluidic chip. 1. A modular fluidic chip comprising:a body configured to have at least one flow channel formed in an inside thereof, and be connected to another modular fluidic chip to allow the at least one flow channel to communicate with a flow channel provided in the other modular fluidic chip.2. The modular fluidic chip of claim 1 , wherein the body includes claim 1 ,a core member in which the at least one flow channel is formed; andat least one connection member provided in the core member so as to be coupled with the other modular fluidic chip.3. The modular fluidic chip of claim 2 , wherein the connection members is configured to be provided integrally with the core member or coupled to and separable from the core member.4. The modular fluidic chip of claim 2 , wherein the connection member is configured to open the flow channel provided at an inside thereof when coupled to the other modular fluidic chip and close the flow channel when separated from the other modular fluidic chip.5. The modular fluidic chip of claim 4 , wherein the connection member is formed of an elastic material claim 4 , and is configured to open the flow channel by being compressed in an axial direction and at the same time claim 4 , expanded in a direction perpendicular to the axial direction when the connection member is subjected to pressure in the axial direction through the other modular fluidic chip coupled to one side thereof claim 4 , and configured to close the flow channel by being restored by an elastic force when the pressure is released.6. The modular fluidic chip of claim 5 , wherein claim 5 , on an inner surface of the connection member claim 5 , opening and ...

SYSTEMS, DEVICES, AND METHODS FOR BODILY FLUID SAMPLE COLLECTION

Bodily fluid sample collection systems, devices, and method are provided. The device may comprise a first portion comprising at least a sample collection channel configured to draw the fluid sample into the sample collection channel via a first type of motive force. The sample collection device may include a second portion comprising a sample container for receiving the bodily fluid sample collected in the sample collection channel, the sample container operably engagable to be in fluid communication with the collection channel, whereupon when fluid communication is established, the container provides a second motive force different from the first motive force to move a majority of the bodily fluid sample from the channel into the container. 1. A device for collecting a bodily fluid sample , the device comprising:a first portion comprising a plurality of capillary sample collection channels;a second portion comprising a plurality of sample containers for receiving the bodily fluid sample collected in the sample collection channels, wherein the sample containers have gas permeable and penetration re-sealable enclosure, wherein the gas permeable closure allows for sample to be transferred from the capillary channels to the containers without having to use a separate motive force.2. A device for collecting a bodily fluid sample , the device comprising:a first portion comprising a plurality of capillary sample collection channels;a second portion comprising a plurality of sample containers for receiving the bodily fluid sample collected in the sample collection channels, wherein the sample containers have gas permeable and penetration re-sealable enclosure, wherein the gas permeable closure allows for sample to be transferred from the capillary channels to the containers without having to use a separate motive force, wherein a flow facilitator to provide a favorable pathway to from the channels into the container.369-. (canceled)70. The device of claim 1 , wherein an ...

Microfluidic Temperature Control

A device includes a microfluidic channel structure on a substrate and a first resistive structure on the substrate to control the temperature of at least the substrate. The first resistive structure is separate from, and independent of the, microfluidic channel structure. In some instances, the device includes a second resistive structure. 1. A biologic test chip comprising:a substrate;a microfluidic channel structure on the substrate;a first resistive structure on the substrate to selectively heat the substrate and to selectively sense a temperature of the substrate to control the temperature of at least the substrate, wherein the first resistive structure is separate from and independent of the microfluidic channel structure.2. The chip of claim 1 , comprising:a plurality of second resistive structures located in the microfluidic channel structure, at least some of the second resistive structures to selectively pump fluid and to selectively apply heat within the microfluidic channel structure,wherein the first resistive structure is electrically independent from the second resistive structures.3. The chip of claim 1 , wherein the first resistive structure has a size claim 1 , a shape claim 1 , a resistivity claim 1 , and a thermal coefficient of resistance that is substantially different than a size claim 1 , a shape claim 1 , a resistivity claim 1 , and a thermal coefficient of resistance of the second resistive structure.4. The chip of claim 1 , wherein the first resistive structure has a length and a shape substantially dissimilar from a shape and length of the microfluidic channel structure.5. The chip of claim 1 , wherein the first resistive structure comprises a plurality of first resistive structures claim 1 , and wherein a first one of the respective first resistive structures has a shape and size generally corresponding to a shape and size of a periphery of the substrate claim 1 , andwherein a second one of the respective first resistive structures has a ...

Screening kit and method

Methods are provided for the large-scale high-content analysis of biological samples. In some embodiments, the methods are implemented in a reversed open microwell system that includes an array of open microwells, at least one microchannel, at least one input port and at least one output port In certain embodiments, the reversed open microwell system can be inserted in an automated management system which includes an incubator at controlled temperature, humidity and CO2 levels, a fluid dispensing system, and is capable of phase-contrast and fluorescence image acquisition. 1. A kit comprising:a tip;a microfluidic device comprising a reversed open microwell system, the reversed open microwell system comprising an array of open microwells, at least one microchannel, at least one input port for at least one reagent and/or for one or more biological samples, and at least one output port for the at least one reagent and/or one or more biological samples, said input and output ports being in microfluidic communication with one or more of said at least one microchannels, wherein said at least one microchannel has a cross-section area of micrometric dimensions and is configured to provide a fluid to microwells of said array of open microwells;{'b': 3', '4', '2', '3', '2', '3', '2', '1', '1, 'wherein said tip comprises a proximal portion intended to cooperate with a fluid dispensing system and a distal portion, said proximal portion of generally tubular configuration and said distal portion is open tapered wherein the terminal base of said distal portion has an outer diameter of dimension d, and the upper base of said distal portion has an outer diameter of dimension d, wherein a distance between said upper base and said terminal base of said distal portion is h, the half-opening of a truncated cone formed by said distal portion is (90°-β), and said proximal portion has a height of h; wherein said input port comprises a vertical channel leading into said at least one ...

RESONATOR AND PROCESS FOR PERFORMING BIOLOGICAL ASSAY

A process for assaying a biological sample includes: receiving a reference sample by an acoustic article including: a resonator including: a substrate; a piezoelectric member; and a phase noise detector; disposing the reference sample on the piezoelectric member; producing a reference phase noise signal; detecting the reference phase noise signal; disposing a biological sample on the piezoelectric member; producing a first biological phase noise signal; detecting the first biological phase noise signal; contacting the biological sample disposed on the piezoelectric member with an antimicrobial agent; producing a second biological phase noise signal; detecting the second biological phase noise signal; and analyzing the first biological phase noise signal, the second biological phase noise signal, and the reference phase noise signal to assay the biological sample. 1. An acoustic article for assaying a biological sample , the article comprising: a substrate comprising an inverted mesa structure;', 'a piezoelectric member disposed on the substrate to receive the biological sample and to produce a phase noise signal, the phase noise signal indicating activity of the biological sample; and, 'a resonator comprisinga phase noise detector in electrical communication with the piezoelectric member to receive the phase noise signal from the piezoelectric member and to produce a phase noise spectrum in response to receiving the phase noise signal from the piezoelectric member.2. The acoustic article of claim 1 , further comprising:a plurality of electrodes disposed on the piezoelectric member to provide excitation of vibration of the piezoelectric member.3. The acoustic article of claim 1 , further comprising:a first fluid channel disposed on the substrate to communicate a fluid comprising the biological sample to the piezoelectric member.4. The acoustic article of claim 3 , further comprising:a second fluid channel disposed on the substrate to communicate the fluid from the ...

Microparticle measurement device

In a microparticle measurement device, a sample is passed through each channel in a multi-flow channel, and a predetermined linear area is illuminated with light. Measurement light originating from a microparticle in the sample, such as scattered or fluorescent light, is shaped into a parallel beam by an objective lens and passes through a first and second transmission portions. The beams transmitted through these two portions are converged as first and second measurement beams onto the same straight line by a cylindrical lens. The intensity of the interference light formed by these beams is detected with a detector. Meanwhile, the light emitted from the light source and passing through the multi-flow channel without hitting the microparticle falls through the objective lens onto a non-reflection portion and does not travel toward the cylindrical lens. Accordingly, only the interference light formed by the measurement beams is allowed to fall onto the detector.

LIGHT-DIGITAL PCR CHAMBER AND LIGHT-DIGITAL PCR DEVICE USING SAME

The present invention relates to a light-digital PCR chamber and a light-digital PCR device. The light-digital PCR chamber comprises: a transparent substrate; a metal thin film layer formed on the transparent substrate; a light shielding layer formed on the metal thin film layer; and a microchannel structure formed on the light shielding layer. The light-digital PCR device comprises a laminate comprising a transparent substrate, a metal thin film layer formed on the transparent substrate, and a light shielding layer formed on the metal thin film layer.

Multiplexed testing strip device

A multiplexed testing strip testing device includes a hydrophobic paper-based strip housing element configured for placement of one or more testing strips. One or more alignment markers on the hydrophobic paper-based housing element are marked to facilitate placement of the one or more testing strips. A cover element is arranged above the strip housing element and includes a machine-readable test identifier on an upper surface. A removably attached filtering element is arranged on the hydrophobic paper-based strip housing element.

Methods, Systems, and Devices Relating to Open Microfluidic Channels

The various embodiments described herein relate to fabricating and using open microfluidic networks according to methods, systems, and devices that can be used in applications ranging from home-testing, diagnosis, and research laboratories. Open microfluidic networks allow the input, handling, and extraction of fluids or components of the fluid into or out of the open microfluidic network. Fluids can be inserted into an open microfluidic channel by using open sections of the open microfluidic network. Passive valves can be created in the microfluidic network, allowing the creation of logic circuits and conditional flow and volume valves. The fluid can be presented via the microfluidic network to diagnostic and analysis components. Fluids and components of the fluid can be extracted from the open microfluidic network via functional open sections that are easily interfaced with other microfluidic networks or common laboratory tools.

EQUAL-LIQUID-LEVEL RESERVOIR AND A MICROFLUIDIC BIOCHIP

A microfluidic biochip includes an equal-liquid-level reservoir disposed on a cover. The equal-liquid-level reservoir includes some tanks that have a substantially same liquid level. Each tank has an opening on a bottom surface, each opening communicating with a corresponding microfluidic channel. 1. An equal-liquid-level reservoir , adapted to a microfluidic biochip , the equal-liquid-level reservoir comprising:a plurality of tanks that have a substantially same liquid level, each said tank having an opening on a bottom surface thereof, and each said opening communicating with a corresponding microfluidic channel.2. The equal-liquid-level reservoir of claim 1 , comprising:a container; andat least one partition wall disposed in the container to partition the container into the plurality of tanks, in a manner that a liquid surface of the plurality of tanks is not blocked by the partition wall such that liquid levels of the plurality of tanks are substantially equal.3. The equal-liquid-level reservoir of claim 1 , comprising:a plurality of containers acting as the plurality of tanks respectively; anda leveling tube disposed below and near a liquid surface of the plurality of tanks such that liquid levels of the plurality of tanks are substantially equal.4. The equal-liquid-level reservoir of claim 1 , further comprising:a blocking wall disposed in one of the plurality of tanks, a top of the blocking wall blocking a liquid surface in said tank, and a bottom of the blocking wall not completely blocking liquid in said tank.5. A microfluidic biochip claim 1 , comprising:a substrate with microfluidic channels formed therein;a cover disposed above the substrate; andan equal-liquid-level reservoir disposed on the cover, the equal-liquid-level reservoir comprising a plurality of tanks that have a substantially same liquid level, each said tank having an opening on a bottom surface thereof, and each said opening communicating with a corresponding microfluidic channel.6. The ...

MICROFLUIDIC DEVICE AND METHOD FOR OPERATING THEREOF

The present invention provides a microfluidic device which comprises a drive module and a microfluidic platform. The drive module further comprises a rotary unit and a vibration unit for driving the microfluidic platform, and the microfluidic platform further comprises multiple microfluidic elements for performing tests. The present invention also provides a method for operating a microfluidic device. The method comprises steps using the rotary unit and steps using the vibration unit to distribute sample in a microfluidic structure. 1. A microfluidic device , comprising:a drive module, containing a rotary unit and a vibration unit; and an injection chamber, for accommodating a sample;', 'a metering chamber, connected with the injection chamber; and', 'a reaction chamber, connected with the measuring chamber, for accommodating a test strip., 'a microfluidic platform, mounting on the drive module, controlled by the rotary unit and the vibration unit, wherein the microfluidic platform contains a center of rotation and at least one microfluidic element, and wherein each microfluidic element further comprises2. The microfluidic device according to claim 1 , wherein the microfluidic platform contains multiple microfluidic elements claim 1 , and wherein at least two injection chamber are integrated with each other.3. The microfluidic device according to claim 1 , wherein each microfluidic element further comprises:an overflow chamber; anda microfluidic channel, connected between the metering chamber and the overflow chamber.4. The microfluidic device according to claim 3 , wherein the metering chamber and the reaction chamber are connected at a first access claim 3 , and the microfluidic channel and the overflow chamber are connected at a second access claim 3 , and wherein the distance from the center of rotation to the first access is equal to or smaller than the distance from the center of rotation to the second access.5. The microfluidic device according to claim 3 , ...

Improved cartridge for use in in-vitro diagnostics and method of use thereof

A cartridge for use in in-vitro diagnostics, the cartridge including a cartridge housing, a cartridge element, disposed within the cartridge housing and defining a plurality of operational volumes, at least some of the plurality of operational volumes being mutually linearly aligned, a fluid solution transporter operative to transfer fluid solutions from at least one of the plurality of operational volumes to at least another of the plurality of operational volumes, the fluid solution transporter including a linearly displaceable transport element operative to sequentially communicate with interiors of the at least some of the plurality of operational volumes and a venter, including a linearly displaceable venting element, operative in coordination with the fluid solution transporter to vent at least one of the plurality of operational volumes.

System and Method for Introducing Sample Material

A system for introducing sample material includes a microfluidic chip and a sample feeding zone disposed on or in the microfluidic chip. The sample feeding zone is configured to introduce the sample material into the microfluidic chip. 1. A system for introducing sample material , comprising:a microfluidic chip; anda sample input region disposed on or in the microfluidic chip, the sample input region configured to introduce the sample material.2. The system as claimed in claim 1 , further comprising a sample removal device.3. The system as claimed in claim 2 , wherein the sample input region on the microfluidic chip comprises an input channel for a part of the sample removal device with the sample material located thereon.4. The system as claimed in claim 3 , wherein the input channel is connected to a microfluidic network at at least one connection site.5. The system as claimed in claim 3 , wherein a sealing element is mounted on the sample removal device claim 3 , the sealing element configured to seal off one or more of an attachment port and the input channel from the environment of the microfluidic chip after the sample removal device has been inserted into the input channel.6. The system as claimed in claim 2 , further comprising a sample input device that receives a part of the sample removal device with the sample material located thereon.7. The system as claimed in claim 6 , wherein the sample input device comprises a receiving body or a receiving space configured to receive a part of the sample removal device with the sample material located thereon.8. The system as claimed in claim 6 , wherein the sample input region on the microfluidic chip comprises a recess configured to apply the sample input device with the part of the sample removal device and with the sample material located thereon.9. The system as claimed in claim 6 , wherein the sample input device comprises a hollow cylinder with an attachment configured for the microfluidic chip.10. The system ...

CENTRIPETAL MICROFLUIDIC PLATFORM FOR LAL REACTIVE SUBSTANCES TESTING

A centripetal microfluidic platform comprised of a microfluidics disc and a reader for testing LAL-reactive substances in fluid samples is provided. The microfluidic disc may comprise at least two testing areas wherein each testing area includes a reservoir portion for receiving at least one fluid sample. The disc may comprise a distribution network portion in fluid communication with the reservoir portion. Each distribution network portion may comprise a distribution network of at least four (4) channels, wherein each channel has a metering portion and at least one analysis chamber portion. The analysis chamber portion may comprise a mixing chamber for mixing samples and reagents and an optical chamber portion that is compatible with an optical reader. The metering portion may be sized to meter an aliquot of the fluid sample for analysis in the analysis chamber portion. At least one analysis chamber portion has at least one reagent isolated therein. The centripetal microfluidic platform further includes a reader for testing fluid samples within a microfluidic disc comprising an enclosure, an optical bench, a centripetal disc drive, and a controller. A method for testing at least one fluid sample for LAL-reactive substances is also provided. 1. A method for testing at least one fluid sample for LAL-reactive substances , said method comprising: [ a reservoir portion for receiving at least one fluid sample, said reservoir portion comprising a reservoir and a reservoir outlet;', 'and a distribution network portion in fluid communication with said reservoir portion;', 'wherein each distribution network portion comprises a distribution network of at least four (4) testing channels, wherein each channel has a metering portion and at least one analysis chamber portion comprising an optical chamber, said metering portion being sized to meter an aliquot of said fluid sample for analysis in said optical chamber; and, 'said microfluidic disc comprises at least two testing ...

Spatial Separation of Particles in a Particle Containing Solution for Biomedical Sensing and Detection

A device and method for analyte detection and analytes in a particulate bearing fluid such as whole blood having an instrument for partitioning the particles from the fluid that is integrated with a detector for analyses of one or more particulate bearing fluid analytes while the particles in the particulate bearing fluid are partitioned. 1) A device for analyzing analytes in a complex particulate fluid , said complex particulate fluid comprising a fluid portion comprising an analyte , and a particulate portion , said device comprising:a microchannel capable of housing a column of the complex particulate fluid, the microchannel comprising at least one analyte detection region;an acoustic transducer capable of generating acoustic waves, said acoustic tranducer aligned with the at least one analyte detection region, said acoustic waves partitioning said particulate portion from said fluid portion in said analyte detection region of said microchannel; and,a detector of said analyte in said fluid portion aligned with the analyte detection region of the microchannel.2) The device of further comprising a fluid flow arrestor for arresting flow of the fluid at the analyte detection region.3) The device of wherein said complex fluid comprises whole blood and said fluid portion comprises plasma.4) The device of wherein said acoustic waves are selected from the group consisting of ultrasonic standing waves claim 1 , surface acoustic waves claim 1 , bulk acoustic waves claim 1 , and acoustic waves with frequency preferably in the range of 2 kHz to 2 GHz.5) The device of further comprising a plurality of acoustic transducers claim 1 , a plurality of analyte detection regions claim 1 , and a plurality of analyte detectors.6) The device of further comprising a fluid collector for collecting fluid after analysis of the analyte.7) The device of wherein said complex fluid collector comprises a microchannel claim 6 , a pocket claim 6 , a dilatation claim 6 , a chamber claim 6 , or a ...

CONTAINER HOLDING MEMBER AND MEDICAL CONTAINER SET

The object of the present invention is to provide a container holding member that is capable of simply, surely, and stably holding a plurality of medical containers, and that is usable efficiently for medical containers. The present invention is a container holding member capable of holding a plurality of bottomed tubular medical containers. The container holding member is provided with a flat plate-like substrate section having a plurality of through holes penetrating one surface and the other surface and a plurality of tubular housing sections each having an upper side opening end on a side of the one surface of the substrate section and a lower side opening end on a side of the other surface and each protruding upward or downward from a hole edge of each of the through holes. The tubular housing sections are each configured to be capable of holding each of the medical containers in a state where an outer bottom surface of each of the medical containers protrudes downward from the lower side opening end when the medical containers are housed in the tubular housing sections. 1. A container holding member capable of holding a plurality of bottomed tubular medical containers , whereinthe container holding member comprises:a flat plate-like substrate section having a plurality of through holes each penetrating one surface and the other surface; anda plurality of tubular housing sections each having an upper side opening end on a side of the one surface of the substrate section and a lower side opening end on a side of the other surface and each protruding upward or downward from a hole edge of each of the through holes, andthe tubular housing sections are each configured to be capable of holding each of the medical containers in a state where an outer bottom surface of each of the medical containers protrudes downward from the lower side opening end when the medical containers are housed in the tubular housing sections.212-. (canceled) The present invention relates to ...

MULTI-FACTOR URINE TEST SYSTEM THAT ADJUSTS FOR LIGHTING AND TIMING

System that enables urine testing in a home environment. A user may apply a urine sample to a card containing multiple tests, and capture an image of the card using a phone; an analysis system executing on the phone or in the cloud may analyze the image and determine test results. The test card and analysis system may compensate for variability in lighting conditions and time of exposure to the urine sample, which are difficult to control in a home environment. The test card may contain fiducial markers of known colors; the analysis system may adjust colors in the captured image based on appearance of these markers. Color adjustments may also compensate for nonuniform lighting across the card. The card may also contain time indicators that change appearance over time after urine is applied, and the analysis system may use these indicators to calculate the time of exposure. 1. A multi-factor urine test system that adjusts for lighting and timing , comprising:{'claim-text': [{'claim-text': ['wherein said plurality of test regions comprise either lateral flow assay regions or colorimetric test regions or both;', {'claim-text': ['react with one or more substances that may be present in a urine sample to which each test region is exposed; and', 'change appearance based on a presence of or a quantity of the one or more substances in the urine sample;'], '#text': 'wherein each test region comprises one or more reagents configured to'}], '#text': 'a plurality of test regions,'}, {'claim-text': 'corner fiducial markers located at a boundary of an area of the test card within which the plurality of test regions are contained;', '#text': 'a plurality of fiducial markers, each fiducial marker of the plurality of fiducial markers comprising a reference color that is measured under a reference lighting condition, and wherein the plurality of fiducial markers comprises'}], '#text': 'a test card comprising'}{'claim-text': ['receive an image of the test card captured after the test ...

Microfluidic Chip for Acoustic Separation of Biological Objects

A microfluidic device for sorting biological objects includes a microfluidic chip including a planar substrate having first and second planar surfaces, the planar substrate including first and second networks of channels recessed respectively from the first and second planar surfaces and fluidically connected by way of at least a through-hole in the planar substrate; a first lid attached to the first planar surface of the planar substrate and substantially covering the first network of channels; and a second lid attached to the second planar surface of the planar substrate and substantially covering the second network of channels; and one or more piezoelectric transducers attached to the first lid and/or the second lid and configured to generate first and second acoustic standing waves in a first linear channel of the first network of channels and a second linear channel of the second network of channels, respectively. 1. A microfluidic device for sorting biological objects comprising:{'claim-text': ['a planar substrate having first and second planar surfaces, the planar substrate including first and second networks of channels recessed respectively from the first and second planar surfaces and fluidically connected in series or parallel by way of at least a through-hole in the planar substrate;', 'a first lid attached to the first planar surface of the planar substrate and substantially covering the first network of channels; and', 'a second lid attached to the second planar surface of the planar substrate and substantially covering the second network of channels; and'], '#text': 'a microfluidic chip including:'}one or more first piezoelectric transducers attached to an exterior of the first lid and configured to generate a first acoustic standing wave having a first wavelength in a first linear channel of the first network of channels.2. The microfluidic device of further comprising one or more second piezoelectric transducers attached to an exterior of the second ...

SAMPLE COLLECTION CHIP

A sample collection chip is disclosed in this application, which includes a chip body. The chip body is provided therein with a collection channel and a sample quantitation cell. The collection channel is in communication with the outside via a sample inlet located in a surface of the chip body, the collection channel has a sample-philic property; and the sample quantitation cell and the collection channel are in communication with each other via a first capillary channel, the first capillary channel has a flow section smaller than a flow section of the collection channel, the first capillary channel has a sample-phobic property, and the sample quantitation cell is in communication with an air outlet located in a surface of the chip body via a second capillary channel. 1. A sample collection chip , comprising a chip body , and the chip body being provided therein with:a collection channel, wherein the collection channel is in communication with an outside via a sample inlet located in a surface of the chip body, and the collection channel has a sample-philic property; anda sample quantitation cell, wherein the sample quantitation cell and the collection channel are in communication with each other via a first capillary channel, the first capillary channel has a flow section smaller than a flow section of the collection channel, the first capillary channel has a sample-phobic property, and the sample quantitation cell is in communication with an air outlet located in the surface of the chip body via a second capillary channel.2. The sample collection chip according to claim 1 , wherein the first capillary channel and the collection channel are in an arc-shaped convergent transition.3. The sample collection chip according to claim 1 , wherein an end claim 1 , in communication with the sample quantitation cell claim 1 , of the first capillary channel is a flared end expanding gradually toward the sample quantitation cell.4. The sample collection chip according to claim ...

SYSTEMS AND METHODS FOR AMPLIFYING NUCLEIC ACIDS

An apparatus for performing a thermocyclic process, such as amplifying DNA, includes a microfluidic chip with a channel formed therein and one or more thermal distribution elements disposed over portions of the chip. Each thermal distribution element is configured to distribute thermal energy from an external thermal energy source substantially uniformly over the portion of the chip covered by the thermal distribution element. The portion of the chip covered by the thermal distribution element thereby comprises a discrete temperature zone. Other temperature zones can be defined by other thermal distribution elements or by portions of the chip not covered by a thermal distribution element. The channel is configured so that a fluid flowing through the channel would enter and exit the different temperature zones a plurality of times, thereby alternately exposing the fluid to the temperature of each zone for a period of time required for the fluid to traverse the zone. 1. A DNA amplification method , comprising: a microfluidic chip having a channel;', 'a first thermal distribution element covering only a first portion of the microfluidic chip; and', 'a second thermal distribution element covering only a second portion of the microfluidic chip that does not over lap with the first portion of the microfluidic chip, wherein the first and second thermal distribution elements are arranged such that there is a gap between the first and second thermal distribution elements, the gap corresponding to a third portion of the microfluidic chip,', 'and wherein the channel is configured such that a fluid flowing through the channel would enter and exit the first, second and third portions of the microfluidic chip a plurality of times;, 'providing an apparatus for amplifying DNA, the apparatus comprisingapplying thermal energy to the first thermal distribution element to generate a first temperature in the first portion of the microfluidic chip;applying thermal energy to the second ...

MICROFLUIDIC CARTRIDGE

The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent. 1. (canceled)2. A microfluidic cartridge comprising: an inlet;', 'a reaction chamber downstream of the inlet;', 'a first channel in fluid communication with the inlet and the reaction chamber; and', 'a second channel downstream of the reaction chamber and in fluid communication with the reaction chamber, wherein the plurality of sample lanes comprise a first bank of sample lanes and wherein a first axis intersects the reaction chambers of the first bank, wherein the plurality of sample lanes comprise a second bank of sample lanes, wherein the reaction chambers of the first bank are a different distance from their respective inlets than the reaction chambers of the second bank are from their respective inlets, wherein a second axis intersects a reaction chamber of the first bank and a reaction chamber of the second bank, wherein the second axis is transverse to the first axis., 'a plurality of sample lanes, each of the sample lanes comprising3. The microfluidic cartridge of claim 2 , wherein the inlets of the sample lanes of the first bank and the inlets of the sample lanes of the second bank alternate.4. The microfluidic cartridge of claim 2 , wherein the inlets are aligned along a third axis.5. The microfluidic cartridge of claim 2 , wherein the inlets of the plurality of sample lanes are aligned.6. The microfluidic cartridge of claim 2 , wherein the inlets ...

FILTRATION ASSEMBLY AND METHOD FOR MICROBIOLOGICAL TESTING

The invention concerns a filtration assembly () for microbiological testing and a method of using the filtration assembly for that purpose. The filtration assembly () comprises a ring-like membrane support () holding a filtration membrane (), a cylindrical reservoir () of which opposite axial ends have openings and one axial opening is removably and fluid-tightly attachable to the membrane support () to define a sample volume adjacent to the filtration membrane () on one axial side of the membrane support (); and a drain member () removably and fluid tightly attachable to the membrane support () to define a drain channel space adjacent to the filtration membrane () on an opposite axial side of the membrane support (). 11. A filtration assembly () for microbiological testing , comprising:{'b': 10', '11, 'a ring-like membrane support () holding a filtration membrane ();'}{'b': 20', '10', '11', '10, 'a cylindrical reservoir () of which opposite axial ends have openings and one axial opening is removably and fluid-tightly attachable to the membrane support () to define a sample volume adjacent to the filtration membrane () on one axial side of the membrane support (); and'}{'b': 30', '10', '11', '10, 'a drain member () removably and fluid tightly attachable to the membrane support () to define a drain channel space adjacent to the filtration membrane () on an opposite axial side of the membrane support ().'}21. The filtration assembly () according to claim 1 , further comprising:{'b': 40', '20', '40', '10', '10, 'a lid device () removably and fluid tightly attachable to the other axial opening of the reservoir () to close the opening, wherein the lid device () is removably and fluid tightly attachable to the membrane support () so as to seal the one axial side of the membrane support () from the environment,'}{'b': 40', '41', '40', '40, 'i': 'a', 'wherein the lid device () has a hinge () for supporting a lid portion () so as to allow selective opening of the lid device ...

SYSTEM FOR GENERATING DROPLETS WITH PUSH-BACK TO REMOVE OIL

System, including methods, apparatus, and kits, for forming and concentrating emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, a droplet well, and a channel network interconnecting the wells. The system also may comprise an instrument configured to operatively receive the device and to create (i) a first pressure differential to produce an emulsion collected in the droplet well and (ii) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well. 1. A system to form and concentrate an emulsion , comprising:a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, and a droplet well, the device also including a channel network having a first channel, a second channel, and third channel that meet one another in a droplet-generation region; and (a) a first pressure differential to drive sample-containing fluid from the sample well to the droplet-generation region via the first channel, continuous-phase fluid from the continuous-phase well to the droplet-generation region via the second channel, and sample-containing droplets from the droplet-generation region to the droplet well via the third channel, such that the droplet well collects an emulsion including sample-containing droplets disposed in continuous-phase fluid, and', '(b) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well via the third channel., 'an ...

FLUORESCENCE DETECTOR FOR MICROFLUIDIC DIAGNOSTIC SYSTEM

The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently. 1. (canceled)2. A system comprising:a worktable configured to receive a consumable microfluidic cartridge;a plurality of heaters aligned along a heater axis and configured to direct heat to the consumable microfluidic cartridge when the consumable microfluidic cartridge is positioned above the plurality of heaters, the plurality of heaters comprising at least a first heater and a second heater;the consumable microfluidic cartridge comprising a first portion comprising a plurality of detection regions within which amplification and detection of target nucleic acid sequences is carried out, the plurality of detection regions comprising at least a first detection region and a second detection region, the plurality of detection regions aligned along a first cartridge axis, wherein the heater axis and the first cartridge axis are substantially parallel when the consumable microfluidic cartridge is positioned above the plurality of heaters,the consumable microfluidic cartridge comprising a second portion comprising a plurality of inlets comprising at least a first inlet and a second inlet, the plurality of inlets aligned along a second cartridge axis substantially parallel to the first cartridge axis, the first inlet configured to be loaded with a first sample, the second inlet configured to be loaded with a second sample that is different than the first sample,wherein the first heater is configured to direct heat to the first detection region in order to ...

SYSTEMS, DEVICES, AND METHODS FOR AGGLUTINATION ASSAYS USING SEDIMENTATION

Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays. 1. A microfluidic device for conducting an agglutination assay , the microfluidic device comprising: a mixing chamber containing coated particles, wherein the coated particles are configured to bind to an analyte of interest;', 'a reservoir containing a density medium, wherein the density medium has a density smaller than a density of at least some of the coated particles;, 'a substrate at least partially defining or supportingwherein the mixing chamber and the reservoir are in fluid communication with each other and arranged such that aggregates of the analyte of interest and the coated particles travel through the density medium responsive to sedimentation forces.2. The microfluidic device of claim 1 , wherein the substrate comprises a disk configured to rotate to generate the sedimentation forces.3. The microfluidic device of claim 1 , wherein the coated particles comprise affinity reagents configured to bind the analyte of interest at multiple locations.4. The microfluidic device of claim 1 , further comprising a valve between the mixing chamber and the reservoir.5. The microfluidic device of claim 1 , further comprising a detection area in fluid communication with the reservoir claim 1 , wherein the detection area is configured for detection of a signal from the aggregates of the analyte of interest and the coated particles.6. The microfluidic device of claim 1 , wherein the substrate at least partially defines a plurality of assay areas for conducting parallel assays. This application is a divisional application of, and discloses subject matter that is related to subject matters disclosed in, co-pending ...

IN VITRO TISSUE PLATE

An in vitro tissue plate may include a well plate, a fluidic plate disposed on a bottom surface of the well plate, and a media manifold disposed on a bottom surface of the fluidic plate. The well plate may have at least two wells, including a tissue well and a waste well. The fluid plate may include a fluid channel extending between and fluidly connecting the tissue well to the waste well. The media manifold may include a one or more media outlets fluidly connected to the fluid channel. A tissue layer may be deposited in the tissue well. The tissue layer may include human cells such as neurovascular cells. 1. An in vitro tissue plate , comprising:a well plate including at least two wells, wherein the at least two wells include a tissue well and a waste well; anda fluidic plate disposed on a bottom surface of the well plate, wherein the fluidic plate includes a fluid channel extending between and fluidly connected to the tissue well and the waste well.2. The in vitro tissue plate of claim 1 , wherein the fluid channel is connected to the tissue well via a plurality of tissue well pores claim 1 , and wherein the fluid channel is connected to the waste well via one or more waste well pores.3. (canceled)4. The in vitro tissue plate of claim 2 , wherein the plurality of tissue well pores and the one or more waste well pores are formed in a pore plate disposed between the well plate and the fluidic plate.5. The in vitro tissue plate of claim 2 , wherein the plurality of tissue well pores and the one or more waste well pores are formed in the well plate.6. The in vitro tissue plate of claim 2 , further comprising a media manifold disposed on a bottom surface of the fluidic plate opposite the well plate claim 2 , wherein the media manifold includes one or more media outlets fluidly connected to one or more media supply pores formed in the fluidic plate and fluidly connected to the fluid channel.7. The in vitro tissue plate of claim 6 , wherein the fluidic plate includes at ...

MICROFLUIDIC ROTOR DEVICE

Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. An apparatus may include a first layer defining a channel, a set of wells, and a cavity. A second layer may be coupled to the first layer. The second layer may include a protrusion extending towards the first layer. The second layer may define an opening configured to receive a fluid. The channel may establish a fluid communication path between the opening and the set of wells. A container may be slidable within the cavity during use. The protrusion may be configured to penetrate a wall of the container. 1. An apparatus , comprising:a first layer defining a channel, a set of wells, and a cavity;a second layer coupled to the first layer, the second layer including at least one protrusion extending towards the first layer, and the second layer defining an opening configured to receive a fluid, wherein the channel establishes a fluid communication path between the opening and the set of wells; anda container slidable within the cavity during use, wherein the protrusion is configured to penetrate a wall of the container.2. The apparatus of claim 1 , wherein the container is configured to hold one or more of a fluid claim 1 , diluent claim 1 , and a reagent.3. The apparatus of claim 2 , wherein the protrusion is tapered.4. The apparatus of claim 1 , wherein the wall of the container includes a membrane claim 1 , and the at least one protrusion is configured to penetrate the membrane of the container as the container is advanced towards the second layer.5. The apparatus of claim 1 , wherein the first layer includes a metering chamber claim 1 , wherein the cavity is in fluid communication with the metering chamber.6. The apparatus of claim 1 , wherein the apparatus is configured to receive a substance including one or more of blood claim 1 , serum claim 1 , plasma claim 1 , and urine. This ...

Systems and methods for performing immunoassays

Provided herein are systems and methods for assays. In particular, provided herein are systems and methods for performing high throughput immunoassays.

METHODS AND APPARATUS FOR POINT-OF-CARE NUCLEIC ACID AMPLIFICATION AND DETECTION

Methods and apparatus are provided for point-of-care nucleic acid amplification and detection. One embodiment of the invention comprises a fully integrated, sample-to-answer molecular diagnostic instrument that optionally may be used in a multiplexed fashion to detect multiple target nucleic acid sequences of interest and that optionally may be configured for disposal after one-time use. The instrument preferable utilizes an isothermal nucleic acid amplification technique, such as loop-mediated isothermal amplification (LAMP), to reduce the instrumentation requirements associated with nucleic acid amplification. Detection of target amplification may be achieved, for example, via detection of a color shift or fluorescence in a dye added to the amplification reaction. Such detection may be performed visually by an operator or may be achieved utilizing an imaging technique, e.g., spectrophotometric imaging. 1. Apparatus for point-of-care amplification and detection of a target nucleic acid sequence , the apparatus comprising:a sample collector configured to collect a sample matrix;a device having at least one microfluidic channel, at least one reaction chamber with nucleic acid amplification reagents, and at least one valve; anda heating element,wherein the at least one microfluidic channel is configured to transport the sample matrix from the sample collector to the at least one reaction chamber, wherein the heating element is configured to heat the nucleic acid amplification reagents after transport of the sample matrix to the at least one reaction chamber in order to amplify the target nucleic acid sequence when contained in the sample matrix, and wherein the at least one valve is configured to prevent backflow of the sample matrix from the at least one reaction chamber through the at least one microfluidic channel during heating.2. The apparatus of claim 1 , wherein the device further comprises at least one sequestered chamber for venting of overflow from the at ...

MICROFLUIDIC CARTRIDGE FOR PROCESSING AND DETECTING NUCLEIC ACIDS

A system, configured to facilitate processing and detection of nucleic acids, the system comprising a process fluid container and a cartridge comprising: a top layer, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a detection chamber, comprises a portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region. 1. A system for processing a sample , the system comprising a cartridge comprising:a first layer comprising a sample port;an intermediate substrate that is coupled to the first layer, partially separated from the first layer by a film layer, and configured to form a sealed waste chamber and a waste port into the sealed waste chamber, wherein the sealed waste chamber directly opposes the first layer across the film layer and defines an external void; anda fluidic pathway, superior to the intermediate substrate and at least partially separated from the sealed waste chamber by a deformable layer, wherein the fluidic pathway is fluidly coupled to the sample port, and wherein the fluidic pathway operates in an occluded mode in which the deformable layer is deformed by an occluder passing through the external void defined by the waste chamber, the occluded mode allowing waste fluid of the sample to be transmitted through the waste port into an interior portion of the sealed waste chamber.2. The cartridge of claim 1 , wherein the fluidic pathway is defined between the ...

FLOW CHANNEL DEVICE AND DETECTION METHOD USING SAME

A flow channel device includes a flow channel section and an introduction channel section. The flow channel section includes a flow channel in which a detection object flows and a wall surface surrounding the flow channel. The introduction channel section includes an introduction channel having a first end connected to the flow channel and a second end connected to an introduction port, and a wall surface surrounding the introduction channel. At least a part of the wall surface surrounding the introduction channel is a curved surface protruding toward the introduction channel. 1. A flow channel device comprising:a flow channel section including a flow channel in which a detection object flows, and a wall surface surrounding the flow channel; andan introduction channel section including an introduction channel having a first end connected to the flow channel and a second end connected to an introduction port, and a wall surface surrounding the introduction channel,wherein in a shortest direction among directions perpendicular to a direction in which the detection object flows in the flow channel, when wall surfaces facing each other with the flow channel sandwiched therebetween, of the wall surface surrounding the flow channel, are defined as a first wall surface and a second wall surface, and a wall surface adjacent to the first wall surface and a wall surface adjacent to the second wall surface, of the wall surface surrounding the introduction channel, are defined as a third wall surface and a fourth wall surface,at least a part of the third wall surface is a curved surface protruding toward the introduction channel, anda distance between the third wall surface and the fourth wall surface is larger at the second end of the introduction channel than at the first end of the introduction channel.2. The flow channel device of claim 1 , wherein the distance between the third wall surface and the fourth wall surface is increased from the first end toward the second end ...

MICRODISPENSING DEVICE AND AUTOMATIC MICRODISPENSING PROCESS

Means and methods for dispensing small amounts of liquid from multi-channel microdispensing devices, suitable for use in automatic processing in biological assays and for the cultivation of cells and tissues, by means of optical control of the dosed liquid by specific light barrier units. 1. A device for the optical control of liquid drops or spurts intermittently dispensed from a microdispensing device having a plurality of dosing channels , the device comprising:at least one light barrier unit assigned to an associated dosing channel of the microdispensing device, each light barrier unit arranged directly with the associated dosing channel on a common carrier, each light barrier unit including:a light source having an inhomogeneous beam profile; anda light sensor having a sensor surface onto which a profiled light beam of the light source projects,wherein each profiled light beam runs transversely with respect to a propagation direction of the dispensed liquid drops or spurts and is wider than the liquid droplet or spurt to be controlled.2. The device according to claim 1 , wherein each one light barrier unit is assigned to each dosing channel.3. The device according to claim 1 , wherein two light barrier units are assigned to each dosing channel claim 1 , each light beams being at an angle of 30° to 150°.4. The device according to claim 1 , further comprising:a programmed evaluation unit is at least connected to a respective light sensor for determining an intensity of the profiled light beam as falling on the sensor surface, anda storage unit and a computing unit for determining a temporal course of intensity and a difference in intensity at a time the liquid droplet or spurt passes and at an instance immediately before or after passing of the liquid droplet or spurt.5. The device according to claim 4 , further comprising:signal and control means for signaling one of deviation from a predeterminable temporal course of intensity and from the intensity difference. ...

Nanochannel Arrays and Their Preparation and Use for High Throughput Macromolecular Analysis

Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.