Vorrichtung und Verfahren zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf Flächengebilde sowie Verwendung der Vorrichtung

Die Erfindung betrifft eine Vorrichtung zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf ein oder mehrere Flächengebilde (5), die mindestens ein zusammenhängendes Element (2) mit einer Vielzahl von Kammern, eine Abdeckung (1) zum Verschließen dieser Kammern und einen Antrieb zum Bewegen des Elements (2) umfaßt und die dadurch gekennzeichnet ist, daß eine gleichmäßige Erwärmung der Flüssigkeiten in den Kammern mit mehr als 1 DEG C/s erreicht werden kann.

Vorrichtung und Verfahren zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf Flächengebilde sowie Verwendung der Vorrichtung

Vorrichtung zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf ein oder mehrere Flächengebilde (5) umfassend mindestens ein zusammenhängendes Element (2) mit einer Vielzahl von Kammern, eine Abdeckung (1) zum Verschließen dieser Kammern und einen Antrieb zum Bewegen des Elements (2), wobei das Flächengebilde (5) zwischen dem Element (2) und der Abdeckung (1) im Bereich der Vertiefungen (3, 4) anzuordnen ist, dadurch gekennzeichnet, daß die Abdeckung (1) und das Element (2) aus einem mit Mikrowellenstrahlung aufheizbaren Werkstoff bestehen, so daß unter Verwendung eines Mikrowellengeräts eine gleichmäßige Erwärmung der Flüssigkeiten in den Kammern mit mehr als 1°C/s erreicht werden kann ...

SIMPLIFIED FABRIC PROCESSING

CONTAINER FOR THE EXECUTION OF MICROWAVE-SUPPORTED OF CHEMICAL REACTIONS WITH SMALL TEST VOLUMES

METHOD AND REACTOR FOR THE AMPLIFICATION OF DNA

COMPOSITIONS AND METHODS FOR PERFORMING BIOLOGICAL REACTIONS

The present invention relates to an apparatus for performing biological reactions. Specifically, the invention relates to an apparatus for performing nucleic acid hybridization reactions on a substrate layer having a multiplicity of oligonucleotide binding sites disposed thereon, using substrates with flexible covers.

Method and system for determining biological properties of samples.

Bei einem Verfahren zur Ermittlung biologischer oder anderer Eigenschaften von Proben mittels eines Mikroplatten-Lesegeräts sind die Proben in Näpfchen (112) einer Mikrotiterplatte (110) angeordnet. Das Verfahren umfasst ein Füllen von Näpfchen einer Mikrotiterplatte mit Proben, die mindestens eine Probensubstanz in einem flüssigen Medium enthalten, ein Aufheizen von Proben mittels einer Heizeinrichtung, ein Anordnen der Mikrotiterplatte in einer Messposition innerhalb einer Messkammer des Mikroplatten-Lesegeräts sowie eine Untersuchung von Proben während und/oder nach dem Aufheizen in mindestens einem Betriebsmodus des Mikroplatten-Lesegeräts durch Messung von aus den Proben emittierter Strahlung. Das Verfahren ist dadurch gekennzeichnet, dass zum Aufheizen der Proben Mikrowellenstrahlung verwendet wird. Ein zur Durchführung des Verfahrens geeignetes System (100) hat eine Heizeinrichtung (190) zum Aufheizen von Proben, die sich in Näpfchen (112) einer Mikrotitierplatte (110) befinden.

Verfahren und System zur Ermittlung biologischer Eigenschaften von Proben.

Bei einem Verfahren zur Ermittlung biologischer oder anderer Eigenschaften von Proben mittels eines Mikroplatten-Lesegeräts sind die Proben in Näpfchen (112) einer Mikrotiterplatte (110) angeordnet. Das Verfahren umfasst ein Füllen von Näpfchen einer Mikrotiterplatte mit Proben, die mindestens eine Probensubstanz in einem flüssigen Medium enthalten, ein Aufheizen von Proben mittels einer Heizeinrichtung, ein Anordnen der Mikrotiterplatte in einer Messposition innerhalb einer Messkammer des Mikroplatten-Lesegeräts sowie eine Untersuchung von Proben während und/oder nach dem Aufheizen in mindestens einem Betriebsmodus des Mikroplatten-Lesegeräts durch Messung von aus den Proben emittierter Strahlung. Das Verfahren ist dadurch gekennzeichnet, dass zum Aufheizen der Proben Mikrowellenstrahlung verwendet wird. Ein zur Durchführung des Verfahrens geeignetes System (100) hat eine Heizeinrichtung (190) zum Aufheizen von Proben, die sich in Näpfchen (112) einer Mikrotitierplatte (110) befinden.

SIMPLIFIED TISSUE PROCESSING

Improved systems and methods for tissue processing are described here. The chemical process and the construction of the apparatus are simplified by using only two different solutions in two separate reaction modules. They are compatible with processing of tissue specimens for genetic analysis, histology, in situantibody binding and hybridization, archival preservation of morphology and nucleic acids, and combinations thereof.

MICROWAVE HEATING-PURPOSE SAMPLE DECOMPOSITION REACTION VESSEL

An object of he invention is to provide a decomposition reaction vessel adapted for microwave decomposition method and allowing visual observation of a sample in the process of reaction from outside and being capable of being used for every microwave generating device without being deformed. As a solving means therefor, it comprises an outer vessel of metal consisting of an outer sleeve having a plurality of slits cut therein and an outer lid removably threadedly fitted to the top of the outer sleeve, and an inner vessel consisting of a bottomed semitransparent inner sleeve and an inner lid seated on the top of the inner sleeve, wherein the inner sleeve is stored in the outer vessel with its outer peripheral surface being in close contact with the inner peripheral surface of the outer sleeve, the inner sleeve and inner lid being pressed by a pressing means so that the interior of the inner vessel is held in a hermetically closed state.

PROCEDURE FOR THE ACTIVATION OF CHEMICAL OR CHEMICO-PHYSICAL PROCESSES BY SIMULTANEOUS USE OF MICROWAVES AND ULTRASONIC PULSES AND CHEMICAL REACTOR FOR IT

Microfluidic apparatus and method for DNA extraction, amplification and analysis

FILTRATION APPARATUS AND ASSOCIATED METHOD FOR MICROWAVE-ASSISTED CHEMISTRY

A method and apparatus are disclosed that are useful in bench top sample preparation and filtration techniques, including those related to microwave assisted extraction and partial digestion. In one aspect the method includes the steps of positioning a microwave-transparent matrix removal tool in a microwave transparent reaction vessel adding a matrix-based composition to the microwave reaction vessel containing the removal tool, applying microwave radiation to the reaction vessel, the matrix-based composition, and the matrix removal tool, and removing the matrix based composition from the reaction vessel using the matrix removal tool. In another aspect the method includes the steps of positioning a matrix based composition that includes at least some liquid in a filter vessel, and applying a moderate over-pressure to the composition upstream of the filter to accelerate the movement of liquid through the filter.

MULTIPLE HOLLOW PLATE WITH EXTENT HEAT ACCUMULATOR

CONTROLLING AND MODERATING MICROWAVE ENERGY IN CONCURRENT MULTIPLE SAMPLE WELL APPLICATIONS

A system for microwave assisted high-throughput chemical processes is disclosed. The system includes a source of microwave radiation, a microwave cavity in communication with the source, and a microtiter plate in the cavity. The microtiter plate is formed of a microwave transparent material and includes a plurality of sample wells. A fluid chamber is defined by the interior of the four walls, the base, the profiles of the wells, and by the top surface of the microtiter plate. A microwave-moderatin g fluid in the chamber helps produce a more uniform microwave field among the individua l wells in the microtiter plate when microwaves are applied in the cavity from the source.

MICROFLUIDIC DEVICE

A microfluidic device, for analysis, comprising: a chip (1) comprising a cavity (3); a light emitting diode or a laser which emits a first electromagnetic radiation (4) for heating a sample placed in the cavity in use; and an analytical assembly (7) configured to record a change in the sample arising from an interaction of the sample with the first source of electromagnetic radiation.

Heater-evaporator

Heater-evaporator has a hollow refrigerator fluid evaporator body with which is incorporated a heater. The body can enclose the heater, which is separate from the body, or the body and the heater can be part of one another. The heater can be heated by any suitable means, to include electrical resistance and/or induction. The invention can be utilized in refrigeration and temperature control, to include, for example, in laboratory test devices requiring refrigeration and temperature control.

MEHRFACHMULDENPLATTE MIT UMFANGSWÄRMESPEICHER

VEREINFACHTE GEWEBEVERARBEITUNG

Simplified tissue processing

Enclosed reaction container and method of use

SIMPLIFIED TISSUE PROCESSING

... ²²²Improved systems and methods for tissue processing are described here. The ²chemical process and the construction of the apparatus are simplified by using ²only two different solutions in two separate reaction modules. They are ²compatible with processing of tissue specimens for genetic analysis, ²histology, in situantibody binding and hybridization, archival preservation of ²morphology and nucleic acids, and combinations thereof.² ...

Apparatus and method for processing a sample using microwave

An apparatus for processing a sample using microwaves comprises: a reaction vessel comprising a chamber which accommodates a refrigerant and a sample and an injection port through which a gas is injected; a microwave source which irradiates microwaves into the chamber; a connector which carries the gas injected through the injection port; and a gas supplier which is located in the chamber and injects the gas carried by the connector to a refrigerant in the chamber. The connector may comprise a gas carrying portion located above the level of the refrigerant in the chamber.

PLATE WITH RECEIVING MULTIPLES WITH RESERVOIR OF HEAT IN THE PERIMETER

COMPOSITIONS AND METHODS FOR PERFORMING BIOLOGICAL REACTIONS

The present invention relates to an apparatus for performing biological reactions. Specifically, the invention relates to an apparatus for performing nucleic acid hybridization reactions on a substrate layer having a multiplicity of oligonucleotide binding sites disposed thereon, using substrates with flexible covers.

Chemical process apparatus and methods

An apparatus 100 for conducting chemical process steps comprising at least two vessels 120 connected by a conduit 110 for passage of fluid between the vessels, wherein said at least two vessels comprise openings 111 to the exterior of the apparatus for introducing chemicals into the apparatus and for removing products from the apparatus and wherein the apparatus comprises electromagnetic heating means 221 for heating the contents of the at least two vessels. Preferably, the apparatus comprises a glass substrate which comprises the vessels or wells wherein each vessel is connected to at least one secondary channel 112 which links with the conduit. The heating means is ideally electromagnetic heating coils wherein a coil is associated with each well. The vessels may be positioned in series or parallel.

DEVICE AND METHOD FOR SIMULTANEOUSLY TESTING THE ACTION OF LIQUIDS ON SURFACE STRUCTURES AND USE OF SAID DEVICE

VESSEL FOR PERFORMING MICROWAVE-ASSISTED CHEMISTRY ON SMALL VOLUMES OF REAGENTS

Improved cycling device and method

COMPOSITIONS AND METHODS FOR PERFORMING BIOLOGICAL REACTIONS

The present invention relates to an apparatus for performing biological reactions. Specifically, the invention relates to an apparatus for performing nucleic acid hybridization reactions on a substrate layer having a multiplicity of oligonucleotide binding sites disposed thereon, using substrates with flexible covers.

METHOD AND REACTOR FOR THE AMPLIFICATION OF DNA

The invention relates to a particular DNA amplification carried out by the principle of the polymerase chain reaction in one or a number of preferably miniaturised reactors and optionally the arrangement thereof as an array. The above permits the continuous production of larger amounts of DNA, for example, for the production of DNA vaccines under the invention conditions of an essentially approximate flow equilibrium. The above also finds application for influencing the specificity or error rate of the DNA amplification by the principle of an in-vitro evolution, for example, in the development of DNA-aptamers.

Method and apparatus for microwave assisted chemical reactions

A method of microwave assisted chemical reaction includes providing a microwavable reaction vessel which contains at least one material in a sample. The sample is heated by microwave energy to elevate the temperature of the reagent and at least partially volatilize the sample to establish a gas phase within the vessel followed by positive cooling of the gas phase to reduce the temperature and responsively reduce the pressure of the gas phase without effecting substantial cooling of the liquid phase. The method may involve employing cooling exteriorly of and adjacent to the gas phase containing portion of the vessel or cooling by means of a coolant flowing within coils disposed in the interior of the vessel or both. The process is preferably a continuous process. The apparatus may be a vessel transparent to microwave energy for receiving the sample. The vessel has space overlying the liquid phase containing portion for a gas phase. Structures for cooling means for positively cooling the ...

SAMPLE TEMPERATURE CONTROLLER

PROBLEM TO BE SOLVED: To provide a sample temperature controller designed to compose a cooling means for carrying out cooling at a high speed so as to sufficiently utilize advantages of microwave heating performing high-speed heating for a sample and further raising the efficiency of temperature control. SOLUTION: This sample temperature controller is designed to control the temperature of the sample 6. The sample temperature controller is composed of each sample container 5 for housing the sample 6 and the cooling means for applying a liquid to the surface of the sample container and cooling the sample with heat of vaporization of the applied liquid. In the process, the cooling means is composed of a jetting means 4 for jetting the liquid onto the surface of the sample container and applying the liquid and a controlling means 7 for controlling the amount of the applied liquid. COPYRIGHT: (C)2006,JPO&NCIPI ...

Enclosed reaction container

Compositions and methods for performing biological reactions

MULTI-WELL PLATE WITH PERIMETRAL HEAT RESERVOIR

A multi-well plate (10) is disclosed for holding chemical compounds for heating in a microwave oven. The plate (10) includes a substantially planar member (12) with wells (18) arranged in an array of rows and columns (24) in its upper surface (16). The plate has heat reservoirs in the form of fluid filled channels or elongated solid bodies (22) positioned lengthwise along the side portions (14) of the plate (10) to reduce temperature gradients among the wells across the length and width of the plate (10). The heat reservoirs prevent or mitigate heat loss from the wells (18) near the side portions (14) of the plate (10) and ensure that the compounds in all of the wells (18) have a uniform temperature.

FILTRATION APPARATUS AND ASSOCIATED METHOD FOR MICROWAVE-ASSISTED CHEMISTRY

A method and apparatus are disclosed that are useful in bench top sample preparation and filtration techniques, including those related to microwave assisted extraction and partial digestion. In one aspect the method includes the steps of positioning a microwave-transparent matrix removal tool in a microwave transparent reaction vessel adding a matrix-based composition to the microwave reaction vessel containing the removal tool, applying microwave radiation to the reaction vessel, the matrix-based composition, and the matrix removal tool, and removing the matrix based composition from the reaction vessel using the matrix removal tool. In another aspect the method includes the steps of positioning a matrix based composition that includes at least some liquid in a filter vessel, and applying a moderate over-pressure to the composition upstream of the filter to accelerate the movement of liquid through the filter.

MICROFLUIDIC APPARATUS AND METHOD FOR DNA EXTRACTION, AMPLIFICATION AND ANALYSIS

An integrated gel based microfluidic sample processing device An integrated gel based microfluidic sample processing device, suitable for forensic investigations at the scene of a crime,comprising a substrate having a plurality of micro-channels to form at least a DNA extraction chamber in fluidic cooperation with an amplification chamber which in turn is in fluidic cooperation with a separation and detection channel; said micro-channels containing a DNA extraction material and gel based reaction reagents necessary for processing said sample; the device further comprising electrical contacts for coupling to an external power source and capable of inducing electro-kinetic manipulation of said gel based reagents and DNA extracted from said sample throughout the device.

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

Microwave heating device for lab on a chip

A microfluidic device for polymerase chain reaction (PCR) processing includes a platform with a microstructure with at least one reaction chamber, and a heating element. The heating element heats at least a part of the at least one reaction chamber. The heating element includes at least one spin torque oscillator (STO) configured to heat the at least a part of the at least one reaction chamber to one or more temperatures for PCR processing.

Pressure vessel

Die vorliegende Erfindung betrifft einen Druckbehälter (1) zur Aufnahme von zu beheizenden Proben (P), wobei der Druckbehälter (1) eine verschließbare Reaktionskammer (2) als Druckraum zum Auslösen und/oder Fördern chemischer und/oder physikalischer Druckreaktionen aufweist, und wobei der Druckbehälter (1) eine Koppelöffnung sowie einen Mikrowellen-durchlässigen Bereich (80) zwischen der Koppelöffnung (K) und der Reaktionskammer (2) aufweist, über die Mikrowellen in die Reaktionskammer (2) eingekoppelt werden können. Der Mikrowellen-durchlässigen Bereich (80) weist ein Druckaufnahmeteil (71) auf, um den Druck der Reaktionskammer (2) gegenüber der Koppelöffnung (K) abzustützen. Der Mikrowellen-durchlässigen Bereich (80) weist alternativ ein Druckaufnahmeteil (71) aus einem keramischen und chemikalienbeständigen Material auf, welches derart ausgebildet ist, dass die physikalischen Eigenschaften des Mikrowellen-durchlässigen Bereichs (80) bezüglich Mikrowellentransparenz und Stabilität während ...

METHOD OF CONTROLLING A FLOW

The present invention relates to a method of controlling a flow in a micro conduit system, and particularly a micro conduit system comprising one or more capillary- stop valves (6,6') wherein the stopping ability of the capillary stop valve is overcome with the help of an acoustic wave source.

Verfahren zur Amplifikation von DNA

Die Erfindung betrifft die Amplifikation von DNA mit einer semikontinuierlich durchgeführten Polymerase-Chain-Reaction (PCR) in einem oder einer Vielzahl von speziellen, bevorzugt miniaturisierten Reaktoren und deren Anordnung als Array. Die Erfindung ermöglicht die Produktion größerer Mengen von DNA, beispielsweise für die Herstellung von DNA-Vakzinen, innerhalb eines weitgehend angenäherten Fließgleichgewichtes. Sie kann für eine Optimierung und zur Beeinflussung der Spezifität bzw. der Fehlerrate bei der DNA-Amplifikation eingesetzt werden.

Compositions and methods for performing biological reactions

CONTROLLING AND MODERATING MICROWAVE ENERGY IN CONCURRENT MULTIPLE SAMPLE WELL APPLICATIONS

A system for microwave assisted high-throughput chemical processes is disclosed. The system includes a source of microwave radiation, a microwave cavity in communication with the source, and a microtiter plate in the cavity. The microtiter plate is formed of a microwave transparent material and includes a plurality of sample wells. A fluid chamber is defined by the interior of the four walls, the base, the profiles of the wells, and by the top surface of the microtiter plate. A microwave-moderating fluid in the chamber helps produce a more uniform microwave field among the individual wells in the microtiter plate when microwaves are applied in the cavity from the source.

DEVICE AND METHOD FOR SIMULTANEOUSLY TESTING THE ACTION OF LIQUIDS (E.G. DETERGENTS OR ANTI-CORROSIVE AGENTS ON SURFACE STRUCTURES (E.G. TEXTILES OR SHEET METAL) AND USE OF SAID DEVICE

The invention relates to a device for simultaneously testing the action of liquids on one or more surface structures (5). Said device comprises at least one continuous element (2) with a plurality of chambers, a cover (1) for closing said chambers and a drive for displacing the element (2). The invention is characterised in that the liquids in the chambers can be heated uniformly at a rate of more than 1 °C/s.

Simplified tissue processing

Improved systems and methods for tissue processing are described here. The chemical process and the construction of the apparatus are simplified by using only two different solutions in two separate reaction modules. They are compatible with processing of tissue specimens for genetic analysis, histology, in situ antibody binding and hybridization, archival preservation of morphology and nucleic acids, and combinations thereof.

System and method of isothermal DNA amplification devices with mobile devices for the cloud service

A portable isothermal amplification device with a portable heat source and a thermal sensor linking to a cloud environment via mobile devices with a mobile application of the present invention for determining the DNA information of samples with a given primer in a manner of point-of-collection (in the field). The cloud environment with mobile devices can provide isothermal amplification information that may include isothermal amplification related data (e.g., genetic information of organism, cancer cells or viruses of interest), analysis methods, solutions provided by experts for access, storage, analysis and consulting. The information may also include gene expression levels of interest, DNA identity of samples, as well as treatment suggestion and professional lists for consulting. In addition, the cloud environment can provide further solution, which is based on the sequence presence information generated from the devices for the end users.

生物学的反応を行う合成物および方法

... 本発明は生物学的反応を行う装置に関する。特に、本発明は、可撓カバーを備えた基板を使用して、多数のオリゴヌクレオチド結合部位を有する基板層上で核酸ハイブリダイゼーション反応を行う装置に関する。 ...

apparatus and method

Device and method for simultaneously testing the action of liquids (e.g. detergents or anti-corrosive agents on surface structures (e.g. textiles or sheet metal) and use of said device

SIMPLIFIED TISSUE PROCESSING

Simplified tissue processing

Improved systems and methods for tissue processing are described here. The chemical process and the construction of the apparatus are simplified by using only two different solutions in two separate reaction modules. They are compatible with processing of tissue specimens for genetic analysis, histology, in situ antibody binding and hybridization, archival preservation of morphology and nucleic acids, and combinations thereof.

Enclosed reaction container and method of use

Method and reactor for the amplification of dna

Method and apparatus for performing biological reactions on a substrate surface

The present invention provides an apparatus for performing biological reactions on a substrate layer having a multiplicity of oligonucleotide binding sites disposed thereon. The invention provides a hybridization chamber wherein nucleic acid hybridization is performed by reacting biological material on a biochip comprising a substrate having an array of oligonucleotide binding sites. The binding sites are associated with an array of 3-dimensional polyacrylamide pads for anchoring the reactants. The arrays are covered with a flexible layer that permits mixing of the hybridization solution on the biochip and detection of hybridization in situ. Fluid inlet and outlet ports in the chamber provide for control of fluid flow into and out of the chamber.

Method of heating liquid medium using microwaves and anions

Provided is a method comprising adding anions having a high charge density to a liquid medium; the liquid medium comprising molecules that hydrogen bond with one another; the anions interacting with the molecules of the liquid medium with a force that is stronger than the forces that produce hydrogen bonding between the molecules of the medium; and heating the liquid medium by irradiating it with microwaves.

FILTRATION APPARATUS AND ASSOCIATED METHOD FOR MICROWAVE-ASSISTED CHEMISTRY

【課題】抽出処理の速度を早くする濾過方法を提供する。 【解決手段】マイクロ波透過マトリクス除去工具をマイクロ波透過反応容器内に配置する段階と、マトリクスベースの組成物を、除去工具の入ったマイクロ波反応容器に加える段階と、マイクロ波を、反応容器、マトリクスベースの組成物、及びマトリクス除去工具に照射する段階と、マトリクスベースの組成物を、反応容器からマトリクス除去工具を使って取り出す段階を含んでいる。別の態様では、少なくとも幾らかの液体を含んでいるマトリクスベースの組成物をフィルター容器内に配置する段階と、フィルターの上流で、組成物に中程度の過剰圧力を掛けて、液体がフィルターを通って移動するのを加速する段階を含んでいる。 【選択図】図１

Druckbehälter mit Magnetscheibe zum Rühren

Druckbehälter (1) zur Aufnahme von zu beheizenden Proben (P), aufweisendeine Reaktionskammer (2) als Druckraum zum Auslösen und/oder Fördern chemischer und/oder physikalischer Druckreaktionen, wobei die Reaktionskammer (2) zur Aufnahme einer Flüssigkeit (5) ausgebildet ist,eine um eine Rotationsachse drehbar in der Reaktionskammer (2) gelagerte Magnetscheibe (8), undeine außerhalb der Reaktionskammer (2) vorgesehene Magnetanordnung (10) zur Erzeugung eines sich rotierenden Magnetfeldes zum rotatorischen Antrieb der Magnetscheibe (8) um ihre Rotationsachse,wobei die Magnetscheibe (8) wenigstens eine sich quer zur Rotationsachse erstreckende Durchgangsbohrung (13) aufweist, welche derart vorgesehen ist, dass in der Reaktionskammer (2) aufgenommene Flüssigkeit (5) durch Rotation der Magnetscheibe (8) durch die Durchgangsöffnung (13) getrieben wird, um die Flüssigkeit (5) zu rühren.

Microfluidic device

Method and apparatus for large-scale spheroid generation

Methods and apparatus for easily and reproducibly producing large numbers of uniform spheroids including spheroid co-cultures by combining vibrational droplet generation with one or more temperature-controlled transition zones.

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

CORE SAMPLE HOLDER FOR MICROWAVE HEATING OF A CORE SAMPLE

The core sample holder for microwave heating of a core sample includes a hollow housing having opposed open first and second ends. A resilient sleeve is disposed within the hollow housing. An annular bladder is disposed within the hollow housing and surrounds the resilient sleeve. The annular bladder is adapted for receiving a liquid. An annular cavity is defined between the outer surface of the annular bladder and the inner surface of the hollow housing and is adapted for receiving a pressurized fluid through at least one pressurized fluid port. First and second caps releasably cover and seal the first and second ends of the hollow housing, respectively. A microwave waveguide passes through the wall of the hollow housing and the annular cavity for transmitting microwave radiation from an external microwave source into the liquid contained within the annular bladder to heat the liquid.

Simplified tissue processing

METHOD AND DEVICE FOR RAPID HOMOGENISATION AND MASS TRANSPORT

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

COVER MEMBER, METHOD AND TREATMENT MODULE FOR TREATING A BIOLOGICAL SAMPLE ON A SUBSTRATE

A cover member for a substrate supporting a biological sample comprises first and second opposing ends, first and second opposing surfaces, a void in the second surface which, when juxtaposed with a substrate, forms a chamber, and a fluid inlet toward the first end and in fluid communication with the void. The void is bounded by void walls having one or more contoured regions for enhancing fluid movement within the chamber. A treatment module for a biological sample comprises the cover member, a support surface for a substrate bearing the biological sample and clamp means operable to releasably retain the cover member in juxtaposition with the substrate for an incubation period. A method for incubating the biological sample with one or more reagents uses the cover member.

Sublimation screening test and apparatus

Disclosed is an apparatus for rapidly screening chemicals for their ability to sublime. The apparatus consists of a heated well plate, where the chemicals are deposited, and a cooled finger plate, holding fingers that penetrate each well. The two plates touch at only the perimeter in order to facilitate a vacuum seal and to reduce any thermal transfer.

FILTRATION APPARATUS AND ASSOCIATED METHOD FOR MICROWAVE-ASSISTED CHEMISTRY

PROBLEM TO BE SOLVED: To meet the need for filtration techniques that are rapid and are easily incorporated with techniques such as extraction and partial digestion. SOLUTION: This method includes steps of: positioning a microwave-transparent matrix removal tool in a microwave transparent reaction vessel 13; adding a matrix-based composition to the microwave transparent reaction vessel containing the removal tool; applying microwave radiation to the reaction vessel, the matrix-based composition and the matrix removal tool; and removing the matrix based composition from the reaction vessel using the matrix removal tool. In another aspect, the method includes steps of: positioning a matrix-based composition including at least some liquid in a filter vessel; and applying a moderate over-pressure to the composition upstream of the filter to accelerate the movement of liquid through the filter. COPYRIGHT: (C)2010,JPO&INPIT ...

Cover member, method and treatment module for treating a biological sample on a substrate

A cover member for a substrate supporting a biological sample comprises first and second opposing ends, first and second opposing surfaces, a void in the second surface which, when juxtaposed with a substrate, forms a chamber, and a fluid inlet toward the first end and in fluid communication with the void. The void is bounded by void walls having one or more contoured regions for enhancing fluid movement within the chamber. A treatment module for a biological sample comprises the cover member, a support surface for a substrate bearing the biological sample and clamp means operable to releasably retain the cover member in juxtaposition with the substrate for an incubation period. A method for incubating the biological sample with one or more reagents uses the cover member.

Microfluidic apparatus and method for DNA extraction, amplification and analysis

CHEMICAL REACTOR WITH PRESSURE RELEASE

The disclosed invention relates to a reaction bottle comprising a container with a container opening and a container interior, a septum associated with the container and configured to releasably seal the container opening, a needle holder associated with the container, the needle holder defining a holder cavity, a needle associated with the needle holder, the needle disposed at least partially within the holder cavity, wherein the septum is deformable between a sealing rest state and a punctured state, and the septum is deformable into puncturable impingement with an end of the needle when the septum is in the punctured state.

Microfluidic apparatus and method for DNA extraction, amplification and analysis

An integrated gel based microfluidic sample processing device An integrated gel based microfluidic sample processing device, suitable for forensic investigations at the scene of a crime,comprising a substrate having a plurality of micro-channels to form at least a DNA extraction chamber in fluidic cooperation with an amplification chamber which in turn is in fluidic cooperation with a separation and detection channel; said micro-channels containing a DNA extraction material and gel based reaction reagents necessary for processing said sample; the device further comprising electrical contacts for coupling to an external power source and capable of inducing electro-kinetic manipulation of said gel based reagents and DNA extracted from said sample throughout the device.

Cover member, method and treatment module for treating a biological sample on a substrate

A cover member for a substrate supporting a biological sample comprises first and second opposing ends, first and second opposing surfaces, a void in the second surface which, when juxtaposed with a substrate, forms a chamber, and a fluid inlet toward the first end and in fluid communication with the void. The void is bounded by void walls having one or more contoured regions for enhancing fluid movement within the chamber. A treatment module for a biological sample comprises the cover member, a support surface for a substrate bearing the biological sample and clamp means operable to releasably retain the cover member in juxtaposition with the substrate for an incubation period. A method for incubating the biological sample with one or more reagents uses the cover member.

MICROFLUIDIC DEVICE

A method for producing a microfluidic device, for analysis, comprising: a chip comprising a cavity; a light emitting diode or a laser which emits a first source of electromagnetic radiation for heating a sample placed in the cavity in use; and an analytical assembly configured to record a change in the sample arising from an interaction of the sample with the first source of electromagnetic radiation.

MICRO-FLUIDIC DEVICE

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

Automated microwave sample digestion system

There is described an automated heating system for heating a sample contained in a sample holder, comprising: a heating chamber having at least one opening and adapted to receive the sample holder; a microwave generator in microwave transfer communication with the heating chamber; a conveyor extending into the heating chamber and adapted to receive and transport the sample holder into and out of the heating chamber; at least one microwave barrier movable between an open position to allow access to the heating chamber and a closed position to prevent microwaves from leaking out of the heating chamber when the microwave generator is activated; and a control unit for controlling the microwave generator and the conveyor.

MULTI-WELL PLATE WITH PERIMETERAL HEAT RESERVOIR

Verfahren und System zur Ermittlung biologischer Eigenschaften von Proben

Bei einem Verfahren zur Ermittlung biologischer oder anderer Eigenschaften von Proben mittels eines Mikroplatten-Lesegeräts sind die Proben in Näpfchen (112) einer Mikrotiterplatte (110) angeordnet. Das Verfahren umfasst ein Füllen von Näpfchen einer Mikrotiterplatte mit Proben, die mindestens eine Probensubstanz in einem flüssigen Medium enthalten, ein Aufheizen von Proben mittels einer Heizeinrichtung, ein Anordnen der Mikrotiterplatte in einer Messposition innerhalb einer Messkammer des Mikroplatten-Lesegeräts sowie eine Untersuchung von Proben während und/oder nach dem Aufheizen in mindestens einem Betriebsmodus des Mikroplatten-Lesegeräts durch Messung von aus den Proben emittierter Strahlung. Das Verfahren ist dadurch gekennzeichnet, dass zum Aufheizen der Proben Mikrowellenstrahlung verwendet wird. Ein zur Durchführung des Verfahrens geeignetes System (100) hat eine Heizeinrichtung (190) zum Aufheizen von Proben, die sich in Näpfchen (112) einer Mikrotitierplatte (110) befinden.

SIMPLIFIED TISSUE PROCESSING

Thermal processing, laboratory device for e.g. exothermic chemical reactions, comprises enclosure with sample holder, microwave heating unit, cooling air supply duct and extraction fan

Vorrichtung zum Behandeln wenigstens eines Stoffes in wenigstens einem Probenbehälter (2), aufweisend – ein Gehäuse (3), das einen mittels Mikrowellen beheizbaren Behandlungsraum (4) umschließt, wobei das Gehäuse (3) eine Einlassöffnung (5) zum Einströmen von Kühlluft von außen aufweist, und wobei die Einlassöffnung (5) das Gehäuse (3) des Behandlungsraums (4), in den die Mikrowellen einkoppelbar sind, nach außen hin öffnet, – einen in dem Behandlungsraum (4) positionierbaren Aufnahmebehälter (6), in dem der wenigstens eine Probenbehälter (2) wenigstens teilweise einsetzbar ist, – Mittel (8) zur Erzeugung eines Unterdrucks in dem Volumen des Behandlungsraums (4), und – Luftleitmittel (9, 13, 10), die bezüglich des wenigstens einen Probenbehälters (2) derart angeordnet sind, dass sie bei Unterdruck die Kühlluft von außen kommend gezielt in den Aufnahmebehälter (6) und an dem darin befindlichen wenigstens einen Probenbehälter (2) vorbei leiten, wobei die Luftleitmittel (9, 13, 10) ein Verbindungselement (9) umfassen, durch...

Constant temperature water bath pot based on microwave heating

MULTI-SAMPLE HOLDER FOR DECOMPOSITION OR EXTRACTION

There is described a sample holder for decomposition or extraction of a sample material, the sample holder comprising: a frame adapted to receive at least two open-ended sample recipients having sealing caps thereon for holding the sample material; and a rack cover releasably securable to the frame and comprising at least two compression caps to be positioned on the sealing caps in order to exert a pressure thereon, thereby forming a pressure-relief valve for each one of the at least two open-ended sample recipients.

A method for heating and temperature controlling test samples with a microwave oven has a concentric heat transfer block and microwave transparent screen and viewing holes for temperature measurement

Mikrowellen-Heizelement, aufweisend: – einer Mikrowellen-transparenten Isolatorabschirmung, und – einen von dem Isolatorabschirmung umgebenes, im wesentlichen rohr- oder tiegelförmiges Heizelement, das wenigstens teilweise aus einem elektrisch leitenden oder einem Halbleitermaterial besteht und zum Beheizen von Probenmaterial bei Einstrahlung von Mikrowellenenergie dient.

MICROFLUIDIC APPARATUS AND METHOD FOR DNA EXTRACTION, AMPLIFICATION AND ANALYSIS

An integrated gel based microfluidic sample processing device An integrated gel based microfluidic sample processing device, suitable for forensic investigations at the scene of a crime,comprising a substrate having a plurality of micro-channels to form at least a DNA extraction chamber in fluidic cooperation with an amplification chamber which in turn is in fluidic cooperation with a separation and detection channel; said micro-channels containing a DNA extraction material and gel based reaction reagents necessary for processing said sample; the device further comprising electrical contacts for coupling to an external power source and capable of inducing electro-kinetic manipulation of said gel based reagents and DNA extracted from said sample throughout the device.

INDEPENDANT HEATING OF SAMPLES IN A SAMPLE HOLDER

There is described a method for heating a sample material in a sample holder, the method comprising receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein; dynamically forming an individual mini microwave cavity around the sample recipient; and applying microwaves generated by at least one microwave generator directly to the sample. 1. A method for heating a sample material in a sample holder , the method comprising:receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein;dynamically forming an individual mini microwave cavity around the at least one sample recipient inside the heating chamber; andapplying microwaves generated by at least one microwave generator directly to the sample in the at least one sample recipient within the mini microwave cavity.2. The method of claim 1 , wherein dynamically forming the mini microwave cavities comprises mating a set of first partial mini cavities with a set of complementary second partial mini cavities.3. The method of claim 2 , wherein the set of first partial mini cavities are in the heating chamber and the set of complementary second partial mini cavities are on the sample holder.4. The method of claim 1 , further comprising mechanically positioning the sample holder in the heating chamber to align the set of first partial mini cavities with the set of complementary second partial mini cavities.5. The method of claim 1 , wherein dynamically forming an individual mini microwave cavity comprises forming a plurality of individual mini microwave cavities around each one of a plurality of sample recipients in the sample holder.6. The method of claim 5 , wherein applying microwaves comprises applying microwaves generated using at least two separate microwave generators.7. The method of claim 6 , wherein applying microwaves ...

PRESSURE-STEPPED CHEMICAL REACTIONS AND ASSOCIATED METHODS, SYSTEMS, APPARATUSES, AND ARTICLES

In an acid digestion reaction performed in a vessel, an elastic lid or septum can be mounted to the mouth of the vessel with a flanged band. In response to determining that a predetermined pressure has been reached in the vessel, a closing force being applied to the lid or septum can be reduced to initiate venting from the vessel. One or more parameters, including the closing force, can be monitored during the venting, and the closing force can be responsively adjusted to optimize the venting. The closing force can be provided by a clamping apparatus that may or may not include a hydraulic system. The clamping apparatus can be configured to increase the closing force in response to increased hydraulic pressure in the hydraulic system. The clamping apparatus can include an engager having an upwardly extending recess extending at least partially around a downwardly protruding portion of the engager.

Thermal processing, laboratory device for e.g. exothermic chemical reactions, comprises enclosure with sample holder, microwave heating unit, cooling air supply duct and extraction fan

A laboratory device for thermal processes, e.g. chemical reactions, comprises an enclosure (3) with a holder (6) for sample containers (2), a microwave heating unit, a cooling air supply duct (9), and an extraction fan (8). Independent claims are also included for the following: (i) a holding frame (6, 7) for mounting sample containers (2) for microwave heating and forced convection cooling, within a thermal enclosure (3, 4); and (ii) successively heating a sample to induce reaction, and then cooling the sample with cooling air or gas, while it remains in the enclosure.

MULTI-WELL PLATE WITH PERIMETERAL HEAT RESERVOIR

A multi-well plate (10) is disclosed for holding chemical compounds for heating in a microwave oven. The plate (10) includes a substantially planar member (12) with wells (18) arranged in an array of rows and columns (24) in its upper surface (16). The plate has heat reservoirs in the form of fluid filled channels or elongated solid bodies (22) positioned lengthwise along the side portions (14) of the plate (10) to reduce temperature gradients among the wells across the length and width of the plate (10). The heat reservoirs prevent or mitigate heat loss from the wells (18) near the side portions (14) of the plate (10) and ensure that the compounds in all of the wells (18) have a uniform temperature. © KIPO & WIPO 2007 ...

DEVICE AND METHOD FOR SIMULTANEOUSLY TESTING THE ACTION OF LIQUIDS ON SURFACE STRUCTURES AND USE OF SAID DEVICE

The invention relates to a device for simultaneously testing the action of liquids on one or more surface structures (5). Said device comprises at least one continuous element (2) with a plurality of chambers, a cover (1) for closing said chambers and a drive for displacing the element (2). The invention is characterised in that the liquids in the chambers can be heated uniformly at a rate of more than 1 °C/s.

METHOD AND DEVICE FOR RAPID HOMOGENISATION AND MASS TRANSPORT

Rapid mixing and homogenisation of reaction mixtures with respect to temperature and chemical concentrations, as well as greatly enhanced mass transport is achieved when the reaction mixture placed in a vassel suitable for centrifugation, and subjected to asymmetric heating, cooling and simultaneous centrifugation at conditions for creating an enhanced flow within said reaction mixture, wherein said flow ensures practically total mixing and homogenisation of the reaction mixture. The invention makes available an improved reaction vessel based on this rapid mixing and homogenisation, as well as their use in different applications where rapid mixing, homogenisation, and enhanced mass transport is desired, such as chemical synthesis, assays etc.

AUTOMATED MICROWAVE SAMPLE DIGESTION SYSTEM

There is described an automated heating system for heating a sample contained in a sample holder, comprising: a heating chamber having at least one opening and adapted to receive the sample holder; a microwave generator in microwave transfer communication with the heating chamber; a conveyor extending into the heating chamber and adapted to receive and transport the sample holder into and out of the heating chamber; at least one microwave barrier movable between an open position to allow access to the heating chamber and a closed position to prevent microwaves from leaking out of the heating chamber when the microwave generator is activated; and a control unit for controlling the microwave generator and the conveyor.

FAST PCR HEATING

Provided herein is a microplate for polymerase chain reaction (PCR), comprising a substrate formed of a material that is susceptible to heating PCR samples upon the application of an electromagnetic field and/or electromagnetic energy to said substrate. The substrate provides a PCR ramp rate of at least 5° C./second upon the application of an electromagnetic field and/or electromagnetic energy to said substrate.

Ofen mit feuerfestem Einsatz

Mikrowellenofen (1) mit feuerfestem und Mikrowellen-durchlässigem Muffelofeneinsatz (2), aufweisend: einen Mikrowellenraum (10), in welchem der einen Probenraum (20) umgebende Muffelofeneinsatz (2) vorgesehen ist, eine Vorrichtung zum Einkoppeln von Mikrowellen in den Mikrowellenraum (10), und eine Ofentür (11) zum Verschließen des Mikrowellenraums (10), welche zwischen einer eine Ofenöffnung (13) des Mikrowellenraums (10) verschließenden Betriebsposition und einer die Ofenöffnung (13) freigebenden Öffnungsposition bewegbar ist, wobei eine Muffelofentür (3) derart mit der Ofentür (11) vorzugsweise elastisch verbunden ist, dass die Muffelofentür (3) eine Probenraumöffnung (22) des Muffelofeneinsatzes (2) in der Betriebsposition der Ofentür (11) verschließt und in der Öffnungsposition der Ofentür (11) freigibt.

MICROFLUIDIC DEVICE

AUTOMATED MICROWAVE SAMPLE DIGESTION SYSTEM

METHODS AND APPARATUS FOR NEAR FIELD IRRADIATION

Irradiation methods and apparatus configured to deliver power, via electromagnetic fields at a variety of frequencies and power levels, in a localized fashion to a target area. In one example, an electromagnetic field generator is disposed on a substrate and configured to deliver power via electromagnetic energy to a thin region proximate to (above) a surface of the substrate, wherein electromagnetic field intensity decreases significantly beyond the thin region. Such methods and apparatus are particularly useful in a wide variety of processes involving chemical and/or physical interactions in connection with a sample of interest located in the thin region. In different aspects, irradiator apparatus may be configured as disposable devices, and/or used in combination with one or more microfluidic or sensing components, for a variety of medical / laboratory / diagnostic methods and instrumentation implementations.

COVER MEMBER, METHOD AND TREATMENT MODULE FOR TREATING A BIOLOGICAL SAMPLE ON A SUBSTRATE

A cover member for a substrate supporting a biological sample comprises first and second opposing ends, first and second opposing surfaces, a void in the second surface which, when juxtaposed with a substrate, forms a chamber, and a fluid inlet toward the first end and in fluid communication with the void. The void is bounded by void walls having one or more contoured regions for enhancing fluid movement within the chamber. A treatment module for a biological sample comprises the cover member, a support surface for a substrate bearing the biological sample and clamp means operable to releasably retain the cover member in juxtaposition with the substrate for an incubation period. A method for incubating the biological sample with one or more reagents uses the cover member.

Pressure vessel

Die vorliegende Erfindung betrifft einen Druckbehälter (1) zur Aufnahme von zu beheizenden Proben (P), wobei der Druckbehälter (1) eine verschließbare Reaktionskammer (2) als Druckraum zum Auslösen und/oder Fördern chemischer und/oder physikalischer Druckreaktionen aufweist, und wobei der Druckbehälter (1) eine Koppelöffnung sowie einen Mikrowellen-durchlässigen Bereich (80) zwischen der Koppelöffnung (K) und der Reaktionskammer (2) aufweist, über die Mikrowellen in die Reaktionskammer (2) eingekoppelt werden können. Der Mikrowellen-durchlässigen Bereich (80) weist ein Druckaufnahmeteil (71) auf, um den Druck der Reaktionskammer (2) gegenüber der Koppelöffnung (K) abzustützen. Der Mikrowellen-durchlässigen Bereich (80) weist alternativ ein Druckaufnahmeteil (71) aus einem keramischen und chemikalienbeständigen Material auf, welches derart ausgebildet ist, dass die physikalischen Eigenschaften des Mikrowellen-durchlässigen Bereichs (80) bezüglich Mikrowellentransparenz und Stabilität während ...

INDEPENDENT HEATING OF SAMPLES IN A SAMPLE HOLDER

There is described a heating system for independently heating at least one sample recipient containing a sample, the sample recipient provided in a sample holder, comprising: a heating chamber having at least one opening and adapted to receive the sample holder; at least one microwave generator for generating microwaves; at least one microwave applicator inside the heating chamber connected to the at least one microwave generator, the at least one microwave applicator comprising an oven cavity portion for creating a mini microwave cavity around a single sample recipient in the sample holder, and for applying microwaves generated by the at least one microwave generator directly to the single sample recipient; and a control unit for controlling the at least one microwave generator.

MICROWAVE HEATING DEVICE FOR LAB ON A CHIP

A microfluidic device for polymerase chain reaction (PCR) processing includes a platform with a microstructure with at least one reaction chamber, and a heating element. The heating element heats at least a part of the at least one reaction chamber. The heating element includes at least one spin torque oscillator (STO) configured to heat the at least a part of the at least one reaction chamber to one or more temperatures for PCR processing. 1. A microfluidic device for polymerase chain reaction (PCR) processing , comprising:a platform with a microstructure with at least one reaction chamber; anda heating element for at least a part of the at least one reaction chamber, the heating element comprising at least one spin torque oscillator (STO) configured to heat the at least a part of the at least one reaction chamber to one or more temperatures for PCR processing.2. The microfluidic device of claim 1 , wherein the heating element comprises an array of STOs.3. The microfluidic device of claim 2 , wherein the array of STOs comprises an array of individual STOs.4. The microfluidic device of claim 2 , wherein the array of STOs comprises and array of stacked STOs.5. The microfluidic device of claim 1 , wherein the heating element comprises a stack of STOs.6. The microfluidic device of claim 1 , wherein the reaction chamber comprises a plurality of zones claim 1 , each zone heated by an individual STO.7. The microfluidic device of claim 1 , wherein the reaction chamber comprises a plurality of zones claim 1 , each zone heated by an array of STOs.8. The microfluidic device of claim 1 , wherein the reaction chamber comprises a plurality of zones claim 1 , each zone heated by a stack of STOs.9. A method of heating a polymerase chain reaction (PCR) solution in a lab on a chip (LOC) claim 1 , comprising:providing a reaction chamber on the LOC;providing the PCR solution to the reaction chamber; andheating at least one part of the reaction chamber of the LOC using at least one ...

Instrument and method for energizing molecules in charged droplets

An instrument for energizing molecules contained in a sample solution may include a droplet generator configured to generate droplets of the sample solution. The droplet generator illustratively has an elongated nozzle defining an orifice at one end thereof from which the droplets exit the droplet generator, and the orifice illustratively defines a first longitudinal axis centrally therethrough. A molecule energizing source is configured to produce a molecule energizing field, and is positioned relative to the nozzle orifice such that the molecule energizing field extends into at least some of the generated droplets along a direction non-parallel with the first longitudinal axis. The molecule energizing field illustratively carries energy which heats at least one of the generated droplets sufficiently to induce structural changes in at least one molecule contained in the at least one of the generated droplets.

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

Fast pcr heating

Provided herein is a microplate for polymerase chain reaction (PCR), comprising a substrate formed of a material that is susceptible to heating PCR samples upon the application of an electromagnetic field and/or electromagnetic energy to said substrate. The substrate provides a PCR ramp rate of at least 5° C./second upon the application of an electromagnetic field and/or electromagnetic energy to said substrate.

INDEPENDANT HEATING OF SAMPLES IN A SAMPLE HOLDER

There is described a method for heating a sample material in a sample holder, the method comprising receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein; dynamically forming an individual mini microwave cavity around the sample recipient; and applying microwaves generated by at least one microwave generator directly to the sample. 1. A method for heating a sample material in a sample holder , the method comprising:receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein;dynamically forming an individual mini microwave cavity around the sample recipient; andapplying microwaves generated by at least one microwave generator directly to the sample.2. The method of claim 1 , wherein dynamically forming the mini microwave cavities comprises mating a set of first partial mini cavities with a set of complementary second partial mini cavities.3. The method of claim 2 , wherein the set of first partial mini cavities are in the heating chamber and the set of complementary second partial mini cavities are on the sample holder.4. The method of claim 1 , further comprising mechanically positioning the sample holder in the heating chamber to align the set of first partial mini cavities with the set of complementary second partial mini cavities.5. The method of claim 1 , wherein dynamically forming an individual mini microwave cavity comprises forming a plurality of individual mini microwave cavities around each one of a plurality of sample recipients in the sample holder.6. The method of claim 5 , wherein applying microwaves comprises applying microwaves generated using at least two separate microwave generators.7. The method of claim 6 , wherein applying microwaves comprises applying microwaves according to an independent heating schedule with sample specific parameters for ...

Specimen test apparatus

According to one embodiment, a specimen test apparatus includes a resonator, a detector, a radiator, and a dielectric. The resonator is configured to house a test container filled with a test solution. The detector detects a detection target substance contained in the test solution in the test container. The radiator is arranged in the resonator and emits electromagnetic waves, which resonate in a specific resonance direction in the resonator, into the resonator. The dielectric is arranged in the resonator at a position near the test container when the test container is placed in the resonator.

Sample temperature controller

Apparatus and method for processing sample using microwave

PURPOSE: A sample processing apparatus using microwave and a method thereof are provided to enable rapid sample treatment of enzyme reaction by continuous output of uniform microwave. CONSTITUTION: A sample processing apparatus using microwave comprises a reaction container(1), a microwave source, a connector(6), and a gas feeding unit(7). The reaction container comprises a chamber(10) and an inlet port(13). The chamber accepts refrigerant and sample. Gas is injected into the inlet port. The microwave source examines microwave within the chamber. The connector transports the gas inserted through the inlet port. The gas feeding unit is located in the chamber and injects gas into the refrigerant in the chamber.

Container for performing microwave-assisted chemistry with small volumes of reagents

Cover member, method and treatment module for treating biological sample on substrate

本发明公开了一种用于支撑生物样本的基板的盖元件，其包括：相对的第一端部和第二端部；相对的第一表面和第二表面；位于第二表面中的空隙，当空隙与基板并置时形成腔室；以及流体入口，朝向第一端部并与空隙流体连通。空隙由空隙壁界定，空隙壁具有用于增强腔室内的流体移动的一个或多个成型区域。本发明还提供了一种用于生物样本的处理模块，其包括：盖元件；支撑表面，用于其上具有生物样本的基板；以及夹持装置，用于将盖元件以与基板并置的方式能释放地保持持续一温育时间段。本发明还提供一种通过一种或多种试剂温育生物样本的方法，该方法使用所述盖元件。

Method and reactor for the amplification of dna

The invention relates to a particular DNA amplification carried out by the principle of the polymerase chain reaction in one or a number of preferably miniaturised reactors and optionally the arrangement thereof as an array. The above permits the continuous production of larger amounts of DNA, for example, for the production of DNA vaccines under the invention conditions of an essentially approximate flow equilibrium. The above also finds application for influencing the specificity or error rate of the DNA amplification by the principle of an in-vitro evolution, for example, in the development of DNA-aptamers.

cover element, method and treatment module to treat a biological sample on a substrate

ELEMENTO DE COBERTURA, MÉTODO E MÓDULO DE TRATAMENTO PARA TRATAR UMA AMOSTRA BIOLÓGICA SOBRE UM SUBSTRATO. Elemento de cobertura para um substrato que sustenta uma amostra biológica, o qual compreende a primeira e segunda extremidades opostas, a primeira e segunda superfícies opostas, um espaço na segunda superfície que, quando justaposta com um substrato, forma uma câmara e uma entrada de fluido na direção da primeira extremidade e em comunicação de fluido com o espaço. O espaço é limitado pelas paredes do espaço apresentando uma ou mais regiões de contorno para melhorar o movimento do fluido dentro da câmara. Um módulo de tratamento para uma amostra biológica compreende o elemento de cobertura, uma superfície de suporte para um substrato portando a amostra biológica e dispositivo de grampo operável para reter de forma amovível o elemento de cobertura em justaposição com o substrato por um período de incubação. Um método para incubar a amostra biológica com um ou mais reagentes emprega o elemento de cobertura. COVERAGE ELEMENT, METHOD AND TREATMENT MODULE TO TREAT A BIOLOGICAL SAMPLE ON A SUBSTRATE. Covering element for a substrate that supports a biological sample, which comprises the first and second opposite ends, the first and second opposite surfaces, a space on the second surface which, when juxtaposed with a substrate, forms a chamber and a fluid inlet towards the first end and in fluid communication with space. The space is limited by the walls of the space presenting one or more contour regions to improve the movement of the fluid within the chamber. A treatment module for a biological sample comprises the covering element, a support surface for a substrate carrying the biological sample and operable clamping device to removably retain the covering element in juxtaposition with the substrate for an incubation period. A method for incubating the biological sample with one or more reagents employs the cover element.

Microfluidic apparatus and method for DNA extraction, amplification and analysis

An integrated gel based microfluidic sample processing device, suitable for forensic investigations at the scene of a crime, including a substrate having a plurality of micro-channels to form at least a DNA extraction chamber in fluidic cooperation with an amplification chamber which in turn is in fluidic cooperation with a separation and detection channel. The micro-channels containing a DNA extraction material and gel based reaction reagents necessary for processing the sample. The device further having electrical contacts for coupling to an external power source and capable of inducing electro-kinetic manipulation of the gel based reagents and DNA extracted from the sample throughout the device.

Method and system for determining biological properties of samples

Verfahren zur Ermittlung von Eigenschaften von Proben mittels eines Mikroplatten-Lesegeräts, wobei die Proben in Näpfchen einer Mikrotiterplatte angeordnet sind, mit folgenden Schritten: Füllen der Näpfchen einer Mikrotiterplatte mit Proben, die mindestens eine Probensubstanz in einem flüssigen Medium enthalten;Aufheizen von Proben mittels einer Heizeinrichtung, wobei zum Aufheizen der Proben Mikrowellenstrahlung verwendet wird;Anordnen der Mikrotiterplatte in einer Messposition innerhalb einer Messkammer des Mikroplatten-Lesegeräts;Untersuchen der Proben vor, während und/oder nach dem Aufheizen in mindestens einem Betriebsmodus des Mikroplatten-Lesegeräts durch Messung von aus den Proben emittierter Strahlung;dadurch gekennzeichnet, dassein nicht-propagierendes Mikrowellen-Evaneszenzfeld erzeugt wird, welches eine räumliche Ausdehnung hat, die eine selektive Aufheizung einzelner Proben erlaubt. A method for determining properties of samples by means of a microplate reader, the samples being arranged in wells of a microtiter plate, comprising the following steps: filling the wells of a microtiter plate with samples which contain at least one sample substance in a liquid medium; heating samples by means of a Heating device, with microwave radiation being used to heat the samples; placing the microtiter plate in a measuring position within a measuring chamber of the microplate reader; examining the samples before, during and / or after heating in at least one operating mode of the microplate reader by measuring from the Samples of emitted radiation; characterized in that a non-propagating microwave evanescence field is generated which has a spatial extent that allows selective heating of individual samples.

Methods and apparatus for near field irradiation

Irradiation methods and apparatus configured to deliver power, via electromagnetic fields at a variety of frequencies and power levels, in a localized fashion to a target area. In one example, an electromagnetic field generator is disposed on a substrate and configured to deliver power via electromagnetic energy to a thin region proximate to (above) a surface of the substrate, wherein electromagnetic field intensity decreases significantly beyond the thin region. Such methods and apparatus are particularly useful in a wide variety of processes involving chemical and/or physical interactions in connection with a sample of interest located in the thin region. In different aspects, irradiator apparatus may be configured as disposable devices, and/or used in combination with one or more microfluidic or sensing components, for a variety of medical / laboratory / diagnostic methods and instrumentation implementations.

Automated microwave sample digestion system

There is described an automated heating system for heating a sample contained in a sample holder, comprising: a heating chamber having at least one opening and adapted to receive the sample holder; a microwave generator in microwave transfer communication with the heating chamber; a conveyor extending into the heating chamber and adapted to receive and transport the sample holder into and out of the heating chamber; at least one microwave barrier movable between an open position to allow access to the heating chamber and a closed position to prevent microwaves from leaking out of the heating chamber when the microwave generator is activated; and a control unit for controlling the microwave generator and the conveyor.

Vessel for performing microwave-assisted chemistry on small volumes of reagents

A micro vial assembly for performing microwave-assisted chemical reactions on small reaction mixture volumes is disclosed, wherein a reaction vessel ( 10 ) is sealed through a diaphragm ( 30 ) that is capped over an open end of the reaction vessel. The reaction vessel mouths in an end plane of a sleeve ( 20 ) surrounding the reaction vessel, the diaphragm being clamped for sealing the open end of the vessel by means of a cap ( 40 ) which is secured to the sleeve. The sleeve provides a radial extension of the reaction vessel in order to bridge the radial distance between a wall of the reaction vessel and other components in a system for performing microwave-assisted chemical reactions.

Amplification/detection kit, photothermal PCR amplification method and microorganism detection method using the same

증폭/검출 키트는, PCR 반응액이 놓이는 샘플 패드, 상기 샘플 패드에 접촉하여 위치하는 전달 패드, 상기 샘플 패드로부터 상기 전달 패드를 통해 흐르는 상기 PCR 반응액이 유입되고, 제1 면에 증착된 금 박막을 포함하는 반응 패드, 및 상기 전달 패드의 제1 면 및 상기 반응 패드의 제1 면을 덮어 밀봉하는 밀봉 테이프를 포함한다. The amplification/detection kit includes a sample pad on which the PCR reaction solution is placed, a transfer pad positioned in contact with the sample pad, the PCR reaction solution flowing from the sample pad through the transfer pad, and gold deposited on the first surface. A reaction pad including a thin film, and a first surface of the transfer pad and a sealing tape covering and sealing the first surface of the reaction pad.

Method and apparatus for microwave assisted chemical reactions

A method of microwave assisted chemical reaction includes providing a microwavable reaction vessel which contains at least one material in a sample. The sample is heated by microwave energy to elevate the temperature of the reagent and at least partially volatilize the sample to establish a gas phase within the vessel followed by positive cooling of the gas phase to reduce the temperature and responsively reduce the pressure of the gas phase without effecting substantial cooling of the liquid phase. The method may involve employing cooling exteriorly of and adjacent to the gas phase containing portion of the vessel or cooling by means of a coolant flowing within coils disposed in the interior of the vessel or both. The process is preferably a continuous process. The apparatus may be a vessel transparent to microwave energy for receiving the sample. The vessel has space overlying the liquid phase containing portion for a gas phase. Structures for cooling means for positively cooling the gas phase to reduce the pressure of the gas phase without effecting substantial cooling of the reagent are provided. These structures for cooling may be contained within the vessel, exteriorly of the vessel or modification of the vessel configuration to facilitate gas phase cooling or combinations thereof. The invention also provides a method and associated apparatus for employing microwave heating and cooling of gases evolving from the heated sample in performing chemical analysis of samples. The invention further contemplates using such a system to purify samples.

Utilize equipment and the method for microwave treatment sample

一种利用微波处理样品的设备，包括：反应容器，包括容纳制冷剂和样品的室、以及通过其注入气体的注入口；微波源，辐射微波到所述室内；连接器，输送通过注入口注入的气体；以及气体供给器，位于所述室内，并且将由连接器输送的气体注入到所述室内的制冷剂。所述连接器可以包括位于所述室内制冷剂的水平面上方的气体输送部。

Simplified tissue processing

Multi-well plate with perimetral heat reservoir

本发明公开了一种用于盛放在微波炉内加热的化合物的多孔板(10)。该板(10)包括基本平的部件(12)，它具有在其上表面以行和列(24)的阵列排列的孔(18)。该板具有蓄热体，蓄热体的形式为沿着板(10)的侧部(14)纵向设置的流体填充的通道或长形实心体(22)，以降低在板(10)的长度方向和宽度方向的孔中的温度梯度。蓄热体可以防止或者减少从靠近板(10)的所述侧部(14)的孔(18)中损失热量，并确保所有孔(18)中的化合物具有相同的温度。

DISHES WITH MULTIPLE PERFORATIONS WITH HEAT RESERVATION

Un plato con perforaciones múltiples para contener un compuesto para ensayos, dicho plato que comprende:un elemento sustancialmente plano limitado por porciones laterales dependientes hacia abajo y con una superficie superior;una pluralidad de perforaciones colocadas en dicho elemento plano y adaptadas para contener muestras de dicho compuesto, cada una de dichas perforaciones con una abertura localizada en dicha superficie superior, el primer grupo de dichas perforaciones en un arreglo adyacente a una de dichas porciones laterales y una primera cámara alongada que se extiende dentro de dicho elemento plano a lo largo de dicha porción lateral y colocado entre dicha porción lateral y dicho primer grupo de perforaciones, dicha primera cámara estando adaptada para contener un fluido con la capacidad de actuar como una reserva de calor y resistir cambios de temperatura de dichas muestras en dicho primer grupo de perforaciones relativo a las muestras en otras dichas perforaciones. Un plato con múltiples perforaciones de acuerdo con la Reivindicación 1, donde dicho elemento plano está hecho de un material transparente para las microondas.<EMI FILE="02046941_1" ID="1" IMF=JPEG >  A plate with multiple perforations to contain a test compound, said plate comprising: a substantially flat element limited by downwardly dependent side portions and with an upper surface; a plurality of perforations placed in said flat element and adapted to contain samples of said composed, each of said perforations with an opening located in said upper surface, the first group of said perforations in an arrangement adjacent to one of said lateral portions and a first elongated chamber extending within said flat element along said lateral portion and placed between said lateral portion and said first perforation group, said first chamber being adapted to contain a fluid with the ability to act as a heat reserve and resist temperature changes of said samples in said first ...

Method and apparatus for performing biological reactions on a substrate surface

The present invention provides an apparatus for performing biological reactions on a substrate layer having a multiplicity of oligonucleotide binding sites disposed thereon. The invention provides a hybridization chamber wherein nucleic acid hybridization is performed by reacting biological material on a biochip comprising a substrate having an array of oligonucleotide binding sites. The binding sites are associated with an array of 3-dimensional polyacrylamide pads for anchoring the reactants. The arrays are covered with a flexible layer that permits mixing of the hybridization solution on the biochip and detection of hybridization in situ. Fluid inlet and outlet ports in the chamber provide for control of fluid flow into and out of the chamber.

Carbon nanotube-coated microwave vessel to improve microwave energy efficiency through hot spot formation and hybrid heating and manufacturing method thereof

다양한 실시예들은, 핫 스팟 형성 및 하이브리드 가열을 통해 마이크로웨이브 에너지 효율을 향상시키는 탄소 나노 튜브로 코팅된 마이크로웨이브 용기 및 그의 제조 방법에 관한 것으로, 탄소 나노 튜브를 포함하는 서셉터가 용기의 표면에 코팅되어, 마이크로웨이브 에너지를 흡수하고, 마이크로웨이브 에너지를 기반으로 열 에너지를 방출함으로써, 시료가 열 에너지에 의해 가열될 수 있다. Various embodiments relate to a microwave container coated with carbon nanotubes for improving microwave energy efficiency through hot spot formation and hybrid heating, and a method for manufacturing the same, wherein a susceptor including carbon nanotubes is applied to the surface of the container. By being coated, absorbing microwave energy and emitting thermal energy based on the microwave energy, the sample can be heated by thermal energy.

Method and apparatus for large-scale spheroid generation

Methods and apparatus for easily and reproducibly producing large numbers of uniform spheroids including spheroid co-cultures by combining vibrational droplet generation with one or more temperature-controlled transition zones.

Multi-sample holder for decomposition or extraction

There is described a sample holder for decomposition or extraction of a sample material, the sample holder comprising: a frame adapted to receive at least two open-ended sample recipients having sealing caps thereon for holding the sample material; and a rack cover releasably securable to the frame and comprising at least two compression caps to be positioned on the sealing caps in order to exert a pressure thereon, thereby forming a pressure-relief valve for each one of the at least two open-ended sample recipients.

Cover member, method for processing biological sample on substrate, and processing module

Probenbehältersystem

Probenbehältersystem (1) zum Einsatz in einem Druckbehälter (100), aufweisend: einen Probenbehälter (2) mit - einem Probenbehälterraum (3) zur Aufnahme einer Probe (P) und - einem Rand (21), der eine Öffnung (22) definiert; und einen Deckel (4) zum Verschließen der Öffnung (22), wobei der Deckel (4) aufweist: - einen Steckabschnitt (41), der über die Öffnung (22) in den Probenbehälter (2) steckbar ist und über den der Deckel (4) relativ zum Probenbehälter (2) axial verfahrbar ist, - einen Flansch (43), der sich ausgehend von dem Steckabschnitt (41) radial nach außen erstreckt und, wenn der Steckabschnitt (41) in dem Probenbehälter (2) eingesteckt ist, mit einer Auflageseite (44) auf dem Rand (21) aufliegen kann, und - eine Aussparung (46), die in einer Außenfläche (42) des Steckabschnitts (41) axial und anschließend in der Auflageseite (44) des Flanschs radial nach außen verläuft, sodass, wenn der Steckabschnitt (41) in den Probenbehälter (2) eingesteckt ist und der Flansch (43) mit der Auflageseite (44) auf dem Rand (21) aufliegt, der Probenbehälterraum (3) über die Aussparung (46) mit der Umgebung (U) des Probenbehältersystems (1) fluidisch verbunden ist.

Verfahren zur aktivierung von chemischen oder chemisch-physikalischen prozessen durch gleichzeitige verwendung von mikrowellen und ultraschallpulsen und chemischer reaktor dafür

Core sample holder for microwave heating of a core sample

The core sample holder for microwave heating of a core sample includes a hollow housing having opposed open first and second ends. A resilient sleeve is disposed within the hollow housing. An annular bladder is disposed within the hollow housing and surrounds the resilient sleeve. The annular bladder is adapted for receiving a liquid. An annular cavity is defined between the outer surface of the annular bladder and the inner surface of the hollow housing and is adapted for receiving a pressurized fluid through at least one pressurized fluid port. First and second caps releasably cover and seal the first and second ends of the hollow housing, respectively. A microwave waveguide passes through the wall of the hollow housing and the annular cavity for transmitting microwave radiation from an external microwave source into the liquid contained within the annular bladder to heat the liquid.

Method of controlling a flow

The present invention relates to a method of controlling a flow in a micro conduit system, and particularly a micro conduit system comprising one or more capillary-stop valves ( 6,6 ′) wherein the stopping ability of the capillary stop valve is overcome with the help of an acoustic wave source.

Vereinfachte gewebeverarbeitung

Pressure-Stepped Chemical Reactions and Associated Methods, Systems, Apparatuses, and Articles

In an acid digestion reaction performed in a vessel, an elastic lid or septum can be mounted to the mouth of the vessel with a flanged band. In response to determining that a predetermined pressure has been reached in the vessel, a closing force being applied to the lid or septum can be reduced to initiate venting from the vessel. One or more parameters, including the closing force, can be monitored during the venting, and the closing force can be responsively adjusted to optimize the venting. The closing force can be provided by a clamping apparatus that may or may not include a hydraulic system. The clamping apparatus can be configured to increase the closing force in response to increased hydraulic pressure in the hydraulic system. The clamping apparatus can include an engager having an upwardly extending recess extending at least partially around a downwardly protruding portion of the engager.

Probenbehältersystem

Die vorliegende Erfindung betrifft ein Probenbehältersystem (1) zum Einsatz in einem Druckbehälter (100), aufweisend einen Probenbehälter (2) mit einem Probenbehälterraum (3) zur Aufnahme einer Probe (P) und einem Rand (21), der eine Öffnung (22) definiert; sowie einen Deckel (4) zum Verschließen der Öffnung (22). Der Deckel (4) wiederum weist auf: einen Steckabschnitt (41), der über die Öffnung (22) in den Probenbehälter (2) steckbar ist und über den der Deckel (4) relativ zum Probenbehälter (2) axial verfahrbar ist, einen Flansch (43), der sich ausgehend von dem Steckabschnitt (41) radial nach außen erstreckt und, wenn der Steckabschnitt (41) in dem Probenbehälter (2) eingesteckt ist, mit einer Auflageseite (44) auf dem Rand (21) aufliegen kann, und eine Aussparung (46), die in einer Außenfläche (42) des Steckabschnitts (41) axial und anschließend in der Auflageseite (44) des Flanschs radial nach außen verläuft, sodass, wenn der Steckabschnitt (41) in den Probenbehälter (2) eingesteckt ist und der Flansch (43) mit der Auflageseite (44) auf dem Rand (21) aufliegt, der Probenbehälterraum (3) über die Aussparung (46) mit der Umgebung (U) des Probenbehältersystems (1) fluidisch verbunden ist. Die Erfindung betrifft ferner ein Druckbehälter (100) aufweisend ein erfindungsgemäßes Probenbehältersystem (1).

Instrument and method for energizing molecules in charged droplets

An instrument for energizing molecules contained in a sample solution may include a droplet generator configured to generate droplets of the sample solution. The droplet generator illustratively has an elongated nozzle defining an orifice at one end thereof from which the droplets exit the droplet generator, and the orifice illustratively defines a first longitudinal axis centrally therethrough. A molecule energizing source is configured to produce a molecule energizing field, and is positioned relative to the nozzle orifice such that the molecule energizing field extends into at least some of the generated droplets along a direction non-parallel with the first longitudinal axis. The molecule energizing field illustratively carries energy which heats at least one of the generated droplets sufficiently to induce structural changes in at least one molecule contained in the at least one of the generated droplets.

Device for the amplification of dna, comprising a microwave energy source

Method and apparatus for large-scale spheroid generation

교반을 위한 자기 디스크를 갖는 압력 용기

본 발명은 가열될 시료(P)를 수용하기 위한 압력 용기(1)에 관한 것으로서, 화학적 및/또는 물리적 압력 반응의 개시 및/또는 촉진을 위한 압력 공간으로서의 반응 챔버(2)로서, 반응 챔버(2)는 액체(5)를 수용하도록 설계되는, 반응 챔버(2); 반응 챔버(2)에서 회전축을 중심으로 회전 가능하게 장착된 자기 디스크(8); 및 이의 회전축을 중심으로 자기 디스크(8)를 회전식으로 구동하기 위한 회전 자기장을 생성하기 위해, 반응 챔버(2)의 외부에 제공된 자석 장치(10)를 가지며, 자기 디스크(8)는 회전축에 대해 횡방향으로 연장되는 적어도 하나의 통로 보어(13)를 갖고, 적어도 하나의 통로 보어(13)는 액체(5)를 교반하기 위해, 반응 챔버(2)에 수용된 액체(5)가 자기 디스크(8)의 회전에 의해 통로 개구부(13)를 통과하는 방식으로 제공된다.

Mehrfachmuldenplatte mit umfangswärmespeicher

Method for activation of chemical or chemical-physical processes by a simultaneous use of microwaves and ultrasonic pulses and chemical reactor that carries out this method

An apparatus (1) for activation of chemical-physical processes comprising a microwave source (40), for example a magnetron or a klystron or a solid state oscillator (FET transistor), operatively connected at one end of a microwave antenna, for example a co-axial antenna (3), at a connector (13). The apparatus, furthermore, comprises a generator of electric current (45) at high frequency connected to an ultrasonic transducer (2) for example by a shielded cable, not shown in the figure. In operative conditions, the antenna (3) and the ultrasonic transducer (2) are arranged substantially at right angles and at direct contact with a reacting mass (15) contained container (10) in order to activate it and then to complete a desired chemical and/or physical process. More in detail, the reacting mass (15) is subject contemporaneously, or alternatively, to an electromagnetic field generated by the antenna (3), diagrammatically indicated (30), and from the ultrasonic field (20) generated by the ultrasonic transducer (2). By arranging the microwave antenna (3) and the ultrasonic transducer (2) in the reacting mass (15) the efficiency of the process is optimized achieving a synergistic effect thanks to the combination of the electromagnetic field and the ultrasonic field.

Method for activation of chemical or chemical-physical processes by a simultaneous use of microwaves and ultrasonic pulses and chemical reactor that carries out this method

An apparatus (1) for activation of chemical-physical processes comprising a microwave source (40), for example a magnetron or a klystron or a solid state oscillator (FET transistor), operatively connected at one end of a microwave antenna, for example a co-axial antenna (3), at a connector (13). The apparatus, furthermore, comprises a generator of electric current (45) at high frequency connected to an ultrasonic transducer (2) for example by a shielded cable, not shown in the FIG. In operative conditions, the antenna (3) and the ultrasonic transducer (2) are arranged substantially at right angles and at direct contact with a reacting mass (15) contained container (10) in order to activate it and then to complete a desired chemical and/or physical process. More in detail, the reacting mass (15) is subject contemporaneously, or alternatively, to an electromagnetic field generated by the antenna (3), diagrammatically indicated (30), and from the ultrasonic field (20) generated by the ultrasonic transducer (2). By arranging the microwave antenna (3) and the ultrasonic transducer (2) in the reacting mass (15) the efficiency of the process is optimized achieving a synergistic effect thanks to the combination of the electromagnetic field and the ultrasonic field.

Flow control and processing cartridge

A flow control and processing cartridge used in a nucleic acid analysis apparatus includes a cartridge body and a reaction chip. The cartridge body includes plural chambers for storing at least one sample and plural biochemical reagents and buffers, and plural channels connected with the plural chambers. The reaction chip is in conjunction with the cartridge body and includes plural detection wells, at least one main fluid channel connected with the detection wells and adapted to dispense the sample into the detection wells, and at least one gas releasing channel connected with the detection wells and adapted to release gas from the detection wells.

Behälter zur durchführung mikrowellenunterstützter chemischer reaktionen bei kleinen reagensvolumen

Method of controlling a flow

The present invention relates to a method of controlling a flow in a micro conduit system, and particularly a micro conduit system comprising one or more capillary- stop valves (6,6') wherein the stopping ability of the capillary stop valve is overcome with the help of an acoustic wave source.

圧力段階的な化学反応ならびに関連する方法、システム、装置および物品

【課題】圧力段階的な化学反応ならびに関連する方法、システム、装置および物品の提供。【解決手段】容器内で行われる酸分解反応において、弾性蓋または隔壁は、フランジ付きバンドによって容器の口に取り付けられることができる。容器内で所定の圧力に達したとの決定に応答して、容器からの通気を開始するために、蓋または隔壁に加えられている閉塞力を減少させることができる。閉塞力を含む１つ以上のパラメータは、通気中に監視されることができ、閉塞力は、通気を最適化するように応答的に調整されることができる。閉塞力は、油圧システムを含んでも含まなくてもよいクランプ装置によって提供されることができる。クランプ装置は、油圧システム内の油圧の増大に応答して閉鎖力を増大させるように構成されることができる。【選択図】なし

Pressure-stepped chemical reactions and associated methods, systems, apparatuses, and articles

In an acid digestion reaction performed in a vessel, an elastic lid or septum can be mounted to the mouth of the vessel with a flanged band. In response to determining that a predetermined pressure has been reached in the vessel, a closing force being applied to the lid or septum can be reduced to initiate venting from the vessel. One or more parameters, including the closing force, can be monitored during the venting, and the closing force can be responsively adjusted to optimize the venting. The closing force can be provided by a clamping apparatus that may or may not include a hydraulic system. The clamping apparatus can be configured to increase the closing force in response to increased hydraulic pressure in the hydraulic system. The clamping apparatus can include an engager having an upwardly extending recess extending at least partially around a downwardly protruding portion of the engager.

Pressure-stepped chemical reactions and associated methods, systems, apparatuses, and articles

In an acid digestion reaction performed in a vessel, an elastic lid or septum can be mounted to the mouth of the vessel with a flanged band. In response to determining that a predetennined pressure has been reached in the vessel, a closing force being applied to the lid or septum can be reduced to initiate venting from the vessel. One or more parameters, including the closing force, can be monitored during the venting, and the closing force can be responsively adjusted to optimize the venting. The closing force can be provided by a clamping apparatus that may or may not include a hydraulic system. The clamping apparatus can be configured to increase the closing force in response to increased hydraulic pressure in the hydraulic system. The clamping apparatus can include an engager having an upwardly extending recess extending at least partially around a downwardly protruding portion of the engager.

マイクロ波加熱用試料分解反応容器

本願発明は、マイクロ波分解法に対応し、反応過程中の試料が外から目視することができ、変形することなく、あらゆるマイクロ波発生装置にも使用できる分解反応容器を提供することを目的とする。その解決手段として、複数のスリットが穿設された外筒と該外筒の上部に着脱可能に螺合される外蓋とからなる金属製の外容器と、有底の半透明の内筒と該内筒の上部に着座する内蓋とからなる内容器とから構成され、前記内筒の外周面は前記外筒の内周面と密着状態で前記外容器に収納され、前記内筒と前記内蓋が押圧手段により押圧されて前記内容器の内部が密閉状態を保持する構造とした。

Device for the amplification of dna, comprising a microwave energy source

The invention relates to a device for the amplification of DNA in a reaction mixture, the device (1) comprising a heated chamber (2) including a rotor (3) for holding a plurality of reaction vessels for reaction mixtures, a drive means for the rotor, a microwave energy source (8) with means for controlled delivery of said energy to the reaction mixtures, and a system (14-16) for determining denaturation of double-stranded DNA. The invention also provides a method for the amplification of a nucleic acid strand. In the first step of the method, a reaction mixture is formed comprising the target nucleic acid strand, nucleotides, a primer, a thermostable nucleic acid polymerase, and, if necessary, a reagent for the detection of denaturation of double-stranded DNA. In the second step, the mixture is incubated at a temperature which allows synthesis of a nucleic acid strand complementary to the target nucleic acid strand. The third step comprises denaturing double-stranded DNA formed in the second step by microwave energisation of the reaction mixture with monitoring of the mixture to determine the denaturation end point. The reaction mixture is allowed to cool to a temperature at which primer anneals to the target nucleic acid strand in the fourth step. The second to fourth steps are repeated until a desired level of amplification is achieved.

Vorrichtung und verfahren zum gleichzeitigen testen der einwirkung von flüssigkeiten (z.b. waschmitteln bzw. korrosionsinhibitoren) auf flächengebilde (z.b. textilien bzw. stahlblech) sowie verwendung der vorrichtung

Die Erfindung betrifft eine Vorrichtung gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf ein oder mehrere Flächengebilde (5) die mindestens ein zusammenhängendes Element (2) mit einer Vielzahl von Kammern, eine Abdeckung (1) zum Verschliessen dieser Kammern und einen Antrieb zum Bewegen des Elements (2) umfasst und die dadurch gekennzeichnet ist, dass eine gleichmässige Erwärmung der Flüssigkeiten in den Kammern mit mehr als 1°C/s erreicht werden kann.

带有搅拌用磁体板的压力容器

本发明涉及带有搅拌用磁体板的压力容器，其用于容纳待加热样本(P)的压力容器(1)，具有：作为压力室的用于引发和/或促成化学和/或物理压力反应的反应室(2)，其中该反应室(2)设计用于容纳液体(5)；可绕转动轴线转动地安装在反应室(2)内的磁体板(8)，和设于反应室(2)外的用于产生旋转磁场以驱动磁体板(8)绕其转动轴线旋转的磁体组件(10)，其中该磁体板(8)具有至少一个横向于转动轴线延伸的如此设置的通孔(13)，容纳在反应室(2)中的液体(5)通过磁体板(8)转动被驱赶经过通孔(13)以搅动液体(5)。

带有搅拌用磁体板的压力容器

本发明涉及带有搅拌用磁体板的压力容器，其用于容纳待加热样本(P)的压力容器(1)，具有：作为压力室的用于引发和/或促成化学和/或物理压力反应的反应室(2)，其中该反应室(2)设计用于容纳液体(5)；可绕转动轴线转动地安装在反应室(2)内的磁体板(8)，和设于反应室(2)外的用于产生旋转磁场以驱动磁体板(8)绕其转动轴线旋转的磁体组件(10)，其中该磁体板(8)具有至少一个横向于转动轴线延伸的如此设置的通孔(13)，容纳在反应室(2)中的液体(5)通过磁体板(8)转动被驱赶经过通孔(13)以搅动液体(5)。

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

Method for activation of chemical or chemical-physical processes by a simultaneous use of microwaves and ultrasonic pulses and chemical reactor that carries out this method

An apparatus (1) for activation of chemical-physical processes comprising a microwave source (40), for example a magnetron or a klystron or a solid state oscillator (FET transistor), operatively connected at one end of a microwave antenna, for example a co-axial antenna (3), at a connector (13). The apparatus, furthermore, comprises a generator of electric current (45) at high frequency connected to an ultrasonic transducer (2) for example by a shielded cable, not shown in the FIG. In operative conditions, the antenna (3) and the ultrasonic transducer (2) are arranged substantially at right angles and at direct contact with a reacting mass (15) contained container (10) in order to activate it and then to complete a desired chemical and/or physical process. More in detail, the reacting mass (15) is subject contemporaneously, or alternatively, to an electromagnetic field generated by the antenna (3), diagrammatically indicated (30), and from the ultrasonic field (20) generated by the ultrasonic transducer (2). By arranging the microwave antenna (3) and the ultrasonic transducer (2) in the reacting mass (15) the efficiency of the process is optimized achieving a synergistic effect thanks to the combination of the electromagnetic field and the ultrasonic field.

Methods and apparatus for incubating droplets

Biopolymers are synthesized on a substrate in a manner that that reduces droplet evaporation including at elevated temperatures. The substrate is enclosed by a lid that form an incubation chamber that is effective to impair evaporation of droplets being dispensed and to provide vapor and temperature adjustments. The biopolymer synthesis may be implemented, for example, as part of the fabrication of a microarray which may be the product of DNA or RNA array printing and for oligonucleotide library synthesis, by chemical and enzymatic procedures.

带有搅拌用磁体板的压力容器

本发明涉及带有搅拌用磁体板的压力容器，其用于容纳待加热样本(P)的压力容器(1)，具有：作为压力室的用于引发和/或促成化学和/或物理压力反应的反应室(2)，其中该反应室(2)设计用于容纳液体(5)；可绕转动轴线转动地安装在反应室(2)内的磁体板(8)，和设于反应室(2)外的用于产生旋转磁场以驱动磁体板(8)绕其转动轴线旋转的磁体组件(10)，其中该磁体板(8)具有至少一个横向于转动轴线延伸的如此设置的通孔(13)，容纳在反应室(2)中的液体(5)通过磁体板(8)转动被驱赶经过通孔(13)以搅动液体(5)。

一种基于微波加热的恒温水浴锅

本发明提供了一种基于微波加热的恒温水浴锅，包括由上部的水浴腔和下部的加热腔构成的壳体，水浴腔内设有导热介质和用于检测导热介质的温度的温度传感器，壳体的侧部设有控制箱，加热腔内设有用于对导热介质进行加热的加热装置，温度传感器和加热装置分别与控制箱连接，加热装置为微波加热部件，加热腔内设有介质导管，介质导管的两端分别与水浴腔连通，介质导管上设有促使导热介质在介质导管内流动的动力装置，动力装置和控制箱连接，微波加热部件设置在介质导管上并对流经介质导管的导热介质进行加热。本发明的基于微波加热的恒温水浴锅，由于加热装置为微波加热部件，能够使得恒温水浴锅内的温度更均匀，恒温控制反应更灵敏。

Independent heating of samples in a sample holder

There is described a method for heating a sample material in a sample holder, the method comprising receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein; dynamically forming an individual mini microwave cavity around the sample recipient; and applying microwaves generated by at least one microwave generator directly to the sample.

Micro-fluidic device

Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.

Method of controlling a flow

The present invention relates to a method of controlling a flow in a micro conduit system, and particularly a micro conduit system comprising one or more capillary-stop valves ( 6,6 ′) wherein the stopping ability of the capillary stop valve is overcome with the help of an acoustic wave source.