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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 1235. Отображено 100.
12-01-2012 дата публикации

Multilayer Hydrodynamic Sheath Flow Structure

Номер: US20120009025A1
Автор: Bernard Bunner, John R. Gilbert, Manish Deshpande
Принадлежит: Cytonome ST LLC

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

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Номер записи: 1
19-01-2012 дата публикации

Microfluidic system including a bubble valve for regulating fluid flow through a microchannel

Номер: US20120015442A1
Автор: John Richard Gilbert, Manish Deshpande, Sebastian Böhm
Принадлежит: Cytonome ST LLC

A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.

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Номер записи: 2
08-03-2012 дата публикации

Platelet aggregation using a microfluidics device

Номер: US20120058500A1
Автор: Arnan Deane Mitchell, Francisco Javier Tovar Lopez, Josie Carberry, Shaun Phillip Jackson, Warwick Scott Nesbitt
Принадлежит: MONASH UNIVERSITY

A microfluidics device to provide real time monitoring of platelet aggregation of a biological sample obtained from a subject. The device comprises a channel configured for passage of the biological sample, the channel comprising a protrusion configured to induce an upstream region of shear acceleration coupled to a downstream region of shear deceleration and defining there-between a region of peak rate of shear, the downstream region of shear deceleration defining a zone of platelet aggregation. The device further comprises a platelet detection means for detecting aggregation of platelets in the zone of aggregation as a result of passage of the biological sample through the channel. Methods to assess real time platelet aggregation of a biological sample obtained from a subject are further described.

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Номер записи: 3
02-08-2012 дата публикации

Three-dimensional (3d) hydrodynamic focusing using a microfluidic device

Номер: US20120196314A1
Автор: Ahmad Ahsan Nawaz, Tony Jun Huang, Xiaole Mao
Принадлежит: PENN STATE RESEARCH FOUNDATION

A microfluidic device comprises inlets for a sample flow and an out-of-plane focusing sheath flow, and a curved channel section configured to receive the sample flow and out-of-plane focusing sheath and to provide hydrodynamic focusing of the sample flow in an out-of-plane direction, the out-of-plane direction being normal to a plane including the curved channel. Examples of the invention also include improved flow cytometers.

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Номер записи: 4
29-11-2012 дата публикации

Sheath flow devices and methods

Номер: US20120301883A1
Автор: Andre' De Fusco, Hyongsok Tom Soh, Jiangrong Karen Qian, Paul Pagano, Paul W. Dempsey, Yanting Zhang
Принадлежит: CYNVENIO BIOSYSTEMS Inc

The invention relates generally to fluid processing and, in particular aspects, processing fluids for detection, selection, trapping and/or sorting of particulate moieties. Sheath flow devices described allow isolation of target species from fluid samples while avoiding non-specific binding of unwanted species to the surfaces of the separation device. Biological fluid processing, detection, sorting or selection of cells, proteins, and nucleic acids is described. The invention finds particular use in diagnostic settings, analyzing a patient's medical condition, monitoring and/or adjusting a therapeutic regimen and producing cell based products.

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Номер записи: 5
03-01-2013 дата публикации

Method and system for transferring and/or concentrating a sample

Номер: US20130000738A1
Автор: Jeffrey R. Krogmeier, Jonathan W. Larson, Robert H. Meltzer
Принадлежит: PathoGenetix Inc

A method of controlling the concentration of a sample flowing through a microchannel is provided. The method includes flowing a sample carrier fluid from a sample carrier channel and into an upstream portion of a microchannel such that the sample carrier fluid flows from the upstream portion and toward a downstream portion of the microchannel, and flowing a sheathing fluid from a sheathing fluid channel and into the upstream portion of the microchannel such that the sheathing fluid flows from the upstream portion and toward the downstream portion of the microchannel, and generally parallel to the sample carrier fluid. The method further includes detecting a concentration of a sample in the sample carrier fluid in the downstream portion of the microchannel, and adjusting a flow rate of at least one of the sample carrier fluid and the sheathing fluid based upon the detected concentration of the sample.

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Номер записи: 6
18-07-2013 дата публикации

Microfluidic device, microfluidic dosing system and method for microfluidic flow measurement and dosing

Номер: US20130183209A1
Автор: Martin Richter, Sebastian KIBLER
Принадлежит: Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV

A microfluidic device for detecting a flow parameter, includes a channel configured within a base body, the channel including a first inlet for feeding a first fluid and a second inlet for feeding a second fluid so as to form a fluid stream having the first and second fluids within the channel, and further including an output for providing the fluid stream on the output side, a first feeder including a micropump associated with the first inlet for selectively feeding the first fluid to the channel, a second feeder associated with the second inlet for feeding the second fluid to the channel; and a detector for detecting, on the basis of a different physical property of the first fluid and the second fluid within the channel, a measurement value dependent on a current flow parameter of the first or second fluid.

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Номер записи: 7
22-08-2013 дата публикации

Acoustic waves in microfluidics

Номер: US20130213488A1
Автор: Achim Wixforth, Adam R. Abate, David A. Weitz, Jeremy Agresti, Lothar Schmid, Thomas Franke
Принадлежит: Harvard College

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.

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Номер записи: 8
05-09-2013 дата публикации

Method and device for high-throughput solution exchange for cell and particle suspension

Номер: US20130228530A1
Автор: Daniel R. Gossett, Dino Di Carlo, Henry T.K. Tse
Принадлежит: UNIVERSITY OF CALIFORNIA

A method of exchanging fluids with suspended particles includes providing a microfluidic device with a first inlet channel operatively coupled to a source of particles and a second inlet channel operatively coupled to an exchange fluid. A transfer channel is connected at a proximal end to the first inlet channel and the second inlet channel. First and second outlet channels are connected to a distal end of the transfer channel. The source of particles is flowed at a first flow rate into the first inlet channel while the exchange fluid is flowed at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5. Particles are collected in one of the first and second outlet channels while fluid substantially free of particles is collected in the other of the first and second outlet channels.

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Номер записи: 9
19-12-2013 дата публикации

Magnetic flow cytometry for high sample throughput

Номер: US20130337497A1
Автор: Mathias Reisbeck, Michael Johannes Helou, Oliver Hayden, Sandro Francesco Tedde
Принадлежит: SIEMENS AG

In a measuring device, a production thereof, and a use thereof for magnetic flow cytometry, a microfluidic channel is disposed along an enrichment route such that a magnetically marked cell sample flowing through the microfluidic channel is aligned to magnetic guide strips, enriched by the magnetic field of a magnet at the floor of the channel, and guided past a sensor. The enrichment route is thereby implemented with the microfluidic channel on the packaging of the semiconductor chip carrying the sensor. This construction ensures a long enrichment route for high throughput of large sample volumes.

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Номер записи: 10
14-01-2021 дата публикации

VASCULAR DEVELOPMENT MONITORING SYSTEMS AND USES THEREOF

Номер: US20210008555A1
Автор: DANIELE Michael, ERB Patrick D.
Принадлежит:

Described herein are devices and systems that can be configured to and/or capable of monitoring and/or controlling fluid flow within microchannels and/or vessels in a microphysiological model. Described herein are aspects of a system configured to monitor and/or control fluid flow in a microfluidic device that can include a manifold device, where the manifold device can include an inlet reservoir, an outlet reservoir, a pressure jumper, a sensor, and a scaffold block that can have a plurality of microfluidic channels and/or vessels. Also described herein are methods of making and using the systems and devices described herein. 1. A system configured to monitor , control , or monitor and control fluid flow in a microfluidic device , the system comprising: an inlet reservoir, wherein the inlet reservoir has an inlet configured to receive a fluid flow;', 'an outlet reservoir, wherein the outlet reservoir has an outlet configured to drain a fluid flow from the manifold device;', 'a pressure jumper; wherein the pressure jumper is coupled to the inlet reservoir and the outlet reservoir;', 'a sensor, wherein the sensor is coupled to the jumper; and', 'a scaffold block having a plurality of microfluidic channels, vessels, or both, configured to transport a fluid, wherein one or more of the plurality of microfluidic channels, vessels, or both, is coupled to the inlet reservoir and/or the outlet reservoir; and, 'a manifold device comprisinga pump, wherein the pump is coupled to the inlet and the outlet of the manifold device.2. The system of claim 1 , further comprising a computer configured to receive and analyze data from the sensor; wherein the computer is coupled to the sensor of the manifold device.3. The system of claim 2 , wherein the computer is coupled to the pump claim 2 , wherein the computer can be further configured to transmit a signal to the pump claim 2 , wherein the computer can be further configured to control a flow rate of fluid through the manifold device ...

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Номер записи: 11
10-01-2019 дата публикации

COMPACT MICROFLUIDIC STRUCTURES FOR MANIPULATING FLUIDS

Номер: US20190009273A1
Автор: AMARIE Dragos, GLAZIER James A., JACOBSON Stephen C.
Принадлежит:

Disclosed is a method and apparatus for manipulating fluids. The apparatus may include a microfluidic structure including inlet channels ( and ) and outlet channels (, and ) oriented among bifurcated (), trifurcated () and merging junctions ( and ). The apparatus splits and merges fluids flowing in the channels to produce successive dilutions of the fluids within the outlet channels. Multiple apparatus may be combined in serial, parallel, combined serial and parallel and/or stacked configurations. One or more apparatus may be used alone or to provide various devices or chambers with the diluted fluids. 1. A method of mixing fluids , comprising:introducing a first fluid into a first inlet channel;introducing a second fluid into a second inlet channel;splitting the first fluid and the second fluid into two channels through a bifurcated junction;merging a first channel of the first fluid with a first channel of the second fluid, thereby forming a mixture of the first and second fluids;splitting the first fluid and the second fluid into a plurality of additional channels through a plurality of bifurcated and trifurcated junctions;merging the first fluid, the second fluid and mixtures thereof into a plurality of additional channels through a plurality of mixing junctions.2. The method of further comprising:causing the first fluid, the second fluid, and mixtures thereof to flow into a gradient chamber.3. The method of claim 2 , further comprising:causing the first fluid, the second fluid, and mixtures thereof to flow into a gradient chamber in a spatial order of decreasing concentration of the first fluid and increasing concentration of the second fluid.4. The method of claim 3 , further comprising:causing the first fluid, the second fluid, and mixtures thereof to flow into a gradient chamber in a spatial order of substantially linearly decreasing concentration of the first fluid and increasing concentration of the second fluid.5. The method of claim 3 , further ...

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Номер записи: 12
09-01-2020 дата публикации

MICROFLUIDIC DEVICE FOR ACCOMMODATING, ISOLATING, TREATING, AND/OR PROCESSING CELLS HAVING AN INLET CHAMBER WITH A CHUTE STRUCTURE

Номер: US20200009568A1
Автор: PODENPHANT JENSEN Marie, Van Der Zaag Pieter Jan, VULDERS ROLAND CORNELIS MARTINUS
Принадлежит:

The present invention relates to a microfluidic device suitable for accommodating, isolating, treating and/or processing cells. The device comprises an inlet chamber in fluid communication with at least one feeding channel being arranged in an essentially horizontal direction. The inlet chamber is suitable for receiving a volume of a liquid sample comprising at least one cell, and has an opening at its upward facing end which has a circular, ellipsoidal or polygonal cross-section. Further, the microfluidic device has a chute structure which defines a flow path for guiding the cells from the inlet chamber into the at least one feeding channel. 2. The microfluidic device according to claim 1 , wherein the sloped surface has a convex curvature suitable for focusing and/or directing the flow path towards the inlet of the at least one feeding channel.3. The microfluidic device according to claim 1 , wherein the chute structure is arranged in the transition section between the inlet chamber and at least one feeding channel claim 1 , and wherein the chute structure further comprises a gradual or stepwise slope in the feeding channel's lower surface.4. The microfluidic device according to claim 1 , wherein the horizontal feeding channel has a circular claim 1 , ellipsoidal or polygonal cross section.5. The microfluidic device according to claim 1 , wherein the chute structure is arranged in the transition section between the inlet chamber and at least one feeding channel claim 1 , and wherein claim 1 , in the transition section claim 1 , the horizontal feeding channel has an initial height of between ≥50 μm and ≤200 μm claim 1 , which height gradually or stepwise tapers to between ≥8 μm and ≤50 μm.6. The microfluidic device according to claim 5 , wherein at least stretches of the inlet chamber and/or the feeding channel comprise an anti-adhesive coating.7. The microfluidic device according to claim 6 , wherein the diameter of the inlet chamber is between ≤4 mm claim 6 , ...

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Номер записи: 13
21-01-2021 дата публикации

METHODS AND APPARATUS FOR SINGLE BIOLOGICAL NANOPARTICLE ANALYSIS

Номер: US20210016276A1
Автор: Chiu Daniel T., FUJIMOTO Bryant S., JUNG Seung Ryoung, WANG Jingang
Принадлежит: UNIVERSITY OF WASHINGTON

The present disclosure relates to methods, systems, and devices for performing analyses of biological nanoparticles. More specifically, the present disclosure relates to methods, systems, and devices for performing single biological nanoparticle size determination on a sample while the biological nanoparticle is in transit through a microfluidic chip. In other aspects, the present disclosure relates to methods, systems, and devices for selectively capturing biological nanoparticles on a coated planar surface, the capturing being facilitated by centrifugation. 1. A method for determining the size of a biological nanoparticle in a fluid sample , the method comprising:providing a microfluidic chip comprising at least one microfluidic channel; introducing the fluid sample into the microfluidic chip, the fluid sample comprising a plurality of biological nanoparticles;flowing a portion of the plurality of biological nanoparticles through the at least one microfluidic channel;illuminating in the at least one microfluidic channel at least one biological nanoparticle from the portion of the plurality of biological nanoparticles on a particle-by-particle basis;detecting a light intensity emitted from the at least one biological nanoparticle; andassigning a size value to the at least one illuminated biological nanoparticle, wherein the biological nanoparticle has a hydrodynamic diameter of less than 1 μm.28-. (canceled)9. The method of claim 1 , wherein the at least one microfluidic channel comprises at least one constriction.10. The method of claim 9 , wherein the detection region is within the at least one constriction claim 9 , and wherein the assigning of the size value comprises the use of the measured light intensity.1117-. (canceled)3. The method of claim 1 , wherein the illuminating of the least one biological nanoparticle comprises using an illumination source having a beam width of less than 2 μm within the at least one microfluidic channel claim 1 , and wherein the ...

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Номер записи: 14
25-01-2018 дата публикации

SYSTEM AND METHOD FOR A BIOMIMETIC FLUID PROCESSING

Номер: US20180021780A1
Автор: Italiano Joseph, Mazutis Linas, Thon Jonathan N., Weitz David A.
Принадлежит:

A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range. 1. A biomimetic microfluidic system comprising:a substrate having a first portion and a second portion;a first channel formed in the first portion of the substrate, the first channel extending from a first input to a first output along a longitudinal dimension, the first channel configured to selectively receive at least one first biological composition at a first channel flow rate, the at least one first biological composition including a biological source material;a second channel formed in the second portion of the substrate, the second channel extending from a second input to a second output along the longitudinal dimension, the second channel configured to selectively receive at least one second biological composition at a second channel flow rate, wherein at least a portion of the first and second channels are substantially parallel;microchannels extending between the first channel and the second channel to create a fluid communication path between the first and second channels, wherein the ...

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Номер записи: 15
23-01-2020 дата публикации

Fluid Flow Device

Номер: US20200023361A1
Автор: David John Brockwell, Nikil KAPUR, Sheena Elizabeth Radford
Принадлежит: UNIVERSITY OF LEEDS

A micro-fluidic flow device and method. The device includes a conduit having an inlet and an outlet distal from the inlet. The conduit further includes a plurality of constrictions each having a reduction in a cross-sectional area of the conduit in a direction from the inlet to the outlet. The constrictions are arranged in series and the reduction in cross-sectional area at each of the constrictions is sufficient to induce extensional flow in a fluid travelling therethrough, such that the maximum strain rate in the extensional flow region is at least 500 s −1 .

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Номер записи: 16
28-01-2021 дата публикации

METHOD FOR MICROBIAL SPECIES DETECTION, QUANTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY IDENTIFICATION

Номер: US20210024973A1
Автор: FINCH Michael D., FONG Kee Onn, KOTIAN Saurabh, Lindborg Beth A., MAHAJAN Advitiya, Munshi Cyrus B.
Принадлежит: Urinary Technologies, Inc.

A method of using microfluidic chips to significantly accelerate the time to identify and quantify microbes in a biological sample and test them for antibiotic resistance, particularly for urinary tract infections. A first microfluidic chip uses antibody or similar probes to identify and quantify any microbes present. The same or a similar chip uses antibody or similar probes to identify microbes with DNA or RNA known to indicate antibiotic resistance. Another microfluidic chip tests for antibiotic susceptibility of any microbes by growing them in very small wells in the presence of antibiotics, reducing the time required for such testing by as much as 95%. Another microfluidic chip runs traditional urinalysis or similar tests. 1. A method for analyzing a biological sample , comprising: i. a plurality of microfluidic channels;', 'ii. at least one inlet connected to each of said microfluidic channels for receiving the biological sample and delivering it to said plurality of microfluidic channels;, 'a. providing a microfluidic microbe detection chip (MDC), the MDC comprisingb. providing a plurality of probe reservoirs, each said reservoir containing a supply of probes which recognize microbial surface molecules, microbial intracellular proteins or microbial DNA or RNA and bind thereto, and wherein each said reservoir is connected to at least one of said microfluidic channels on the MDC to deliver said probes in said reservoir to said at least one of said microfluidic channels;c. providing a biological sample to said at least one inlet of the MDC, which in turn provides the sample to said plurality of microfluidic channels;d. causing the probes to engage said biological sample in said plurality of microfluidic channels, whereby the probes can react and bind to said microbial surface molecules, microbial intracellular proteins or microbial DNA or RNA recognized by the probes, if such are present in said biological sample;e. measuring the presence of any of said probes ...

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Номер записи: 17
23-01-2020 дата публикации

METHODS AND DEVICES FOR MULTI-STEP CELL PURIFICATION AND CONCENTRATION

Номер: US20200025669A1
Автор: "DSILVA Joseph", AURICH Lee, Austin Robert H., CAMPOS-GONZALEZ Roberto, CIVIN Curt, GANDHI Khushroo, GRISHAM Michael, SKELLEY Alison, STURM James C., WARD Anthony
Принадлежит:

Described herein are microfluidic devices and methods that can separate and concentrate particles in a sample. 1. A system for separating particles in a sample , comprising:a) one or more microfluidic channels for containing fluid flow in a first direction comprising an array of obstacles arranged in rows and columns, wherein the array of obstacles is configured to differentially separate particles based on size; andb) a dispense module in communication with the one or more microfluidic channels configured to dispense a droplet containing one or more particles, wherein the one or more particles are separated by the array of obstacles in the one or more microfluidic channels.2. The system of claim 1 , wherein the rows define an array direction that differs from the first direction by a tilt angle (ε) of 1/10 radian and wherein the rows repeat periodically and have a periodocity equal to 10 (1/ε).3. The system of claim 1 , wherein the array has a critical size of 4-6 μm.4. The system of claim 1 , wherein the obstacles are circular in shape.5. The system of claim 1 , wherein the one or more microfluidic channels have at least two inlets.6. The system of claim 1 , wherein the dispense module is configured to dispense one particle per droplet.7. The system of claim 1 , further comprising a polymerase chain reaction (PCR) device downstream of the dispense module.8. A method for separating particles in a fluid sample comprising passing said fluid sample through the system of .9. The method of claim 8 , wherein the fluid sample comprises T cells.10. The method of claim 9 , wherein the T cells are CD8 cells.11. The method of claim 9 , wherein at least some of the T cells are bound to magnetic particles.12. The method of claim 11 , wherein the magnetic particles have a diameter of 500 nm.13. The method of claim 8 , further comprising passing a buffer stream in parallel with the fluid sample through the system.14. The method of claim 8 , further comprising performing reverse ...

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Номер записи: 18
02-02-2017 дата публикации

CHIP DEVICE AND A PARTICLE ANALYZING APPARATUS

Номер: US20170028398A1
Автор: Akiyama Shoji, AKIYAMA Yuji, Yamasaki Takeshi
Принадлежит:

A chip device is provided. The chip device includes a flow channel configured to pass a fluid therein; an ejection portion including an opening toward an end face of a substrate layer including at least one layer, the ejection portion is configured to provide the fluid from the flow channel, and a cavity provided between the opening of the ejection portion and the end face of the substrate layer, wherein at least a portion of the cavity is provided at the end face of the substrate layer. 1. A chip device comprising:a flow channel configured to pass a fluid therein;an ejection portion including an opening toward an end face of a substrate layer including at least one layer, the ejection portion is configured to provide the fluid from the flow channel, anda cavity provided between the opening of the ejection portion and the end face of the substrate layer,wherein at least a portion of the cavity is provided at the end face of the substrate layer.2. The chip device according to claim 1 , further comprising a connection portion having a linear shape claim 1 , wherein the connection portion is configured to connect the flow channel with the ejection portion.3. The chip device according to claim 2 , wherein the substrate layer is formed by injection molding.4. The chip device according to claim 3 , wherein the substrate layer is formed by thermocompression bonding.5. The chip device according to claim 4 , whereinthe cavity has a width of 0.2 millimeters or more in corresponding with a distance between the opening of the ejection portion and the end face of the substrate layer.6. The chip device according to claim 5 , whereinthe chip device is configured to be used in a particle analyzing apparatus.7. The chip device according to claim 1 , whereinthe cavity is broader than the opening of the ejection portion.8. The chip device according to claim 1 , wherein the cavity includes an octagonal shape.9. The chip device according to claim 1 , further comprising a suction channel ...

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Номер записи: 19
01-02-2018 дата публикации

Microfluidic Metering of Fluids

Номер: US20180029037A1
Автор: Oppenheimer Aaron, Weber Lutz, Zamir Lee
Принадлежит: Daktari Diagnostics, Inc.

This document provides methods and devices for metering fluids. In some cases, the methods and devices include intersecting channels that include capillary-stop geometries at each intersection point that guides the fluids on a desired path, which is controlled by the opening and closing of valves. For example, a metering channel can intersect a loading channel and intersect an outflow channel and a metering portion can be defined by the geometry of the metering channel between the intersection points. 1. A device for metering fluids , comprising:(a) a metering channel having a metering inlet and a metering outlet;(b) an outflow channel intersecting the metering channel at a metering-outflow intersection point, the outflow channel having an outflow outlet; and(c) a loading channel intersecting the metering channel at a loading-metering intersection point, the loading channel having a loading inlet,the metering channel defining a volume of fluid to be metered between the metering-outflow intersection point and the loading-metering intersection point.2. The device of claim 1 , wherein one or more of said inlets comprise a valve.3. The device of claim 1 , wherein one or more of said outlets comprise a valve.4. The device of claim 1 , wherein one or more of said intersection points comprises capillary-stop geometry.5. The device of claim 1 , wherein the metering-outflow intersection point comprises capillary-stop geometry.6. The device of claim 1 , wherein the metering inlet comprises a valve claim 1 , the metering outlet comprises a valve claim 1 , the loading channel comprises a valve claim 1 , and the metering-outflow intersection point comprises capillary-stop geometry.7. The device of claim 1 , further comprising a controller configured to meter a first predetermined volume of a metered fluid through said outflow channel claim 1 , wherein the controller is configured to:(a) deliver a volume of a loading fluid through the loading channel to fill the loading channel ...

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Номер записи: 20
02-02-2017 дата публикации

DEVICES AND METHODS FOR SEPARATING PARTICLES

Номер: US20170029782A1
Автор: Mao Leidong, Schroeder Carsten, Zhao Wujun
Принадлежит:

Devices for non-invasive, label-free separation of particles in liquid, including circulating tumors cells in blood, are provided. Embodiments of the disclosure provide for devices employing magnetic fluids and magnets for separation of circulating tumor cells from blood. Methods for separation of particles including circulating tumor cells are also provided. 1. A device , comprising:a serpentine microfluidic channel having a first end and a second end,a first inlet at the first end of the serpentine microfluidic channel, wherein the first inlet is configured to flow a first fluid into the serpentine microfluidic channel, wherein the fluid includes a plurality of components,a filtration region disposed between the first end and the second end, wherein the filtration region includes at least one filter in the serpentine microfluidic channel, wherein the filter removes one or more of the plurality of components,a second inlet located after the first inlet and after the filtration region, wherein the second inlet is configured to combine a second fluid with the first fluid to create a third fluid, and to hydrodynamically focus the components of the third fluid into a stream by sheath flow, wherein the second fluid includes a magnetic fluid,one or more permanent magnets positioned adjacent and along the length of an area of the serpentine microfluidic channel after the second inlet, wherein the permanent magnets are positioned so that the magnetic field produces a magnetization direction perpendicular to the flow of fluid in the serpentine microfluidic channel, andtwo or more outlet channels positioned after the one or more permanent magnets at the second end.2. The device of claim 1 , wherein the first fluid includes whole blood claim 1 , wherein the whole blood includes a plurality of components.3. The device of claim 2 , wherein the whole blood includes unlabeled rare cells claim 2 , wherein the device is configured to separate the unlabeled rare cells from other ...

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Номер записи: 21
17-02-2022 дата публикации

SYSTEMS FOR ISOLATING AND TRANSPLANTING PANCREATIC ISLETS

Номер: US20220048030A1
Автор: Brayman Kenneth, Langman Linda W., Peirce-Cottler Shayn, Swami Nathan, VARHUE Walter
Принадлежит:

A microfluidic device can include an upstream passage, a sample passage, a bifurcating passage, and a combining passage. The upstream passage can be configured to provide a focusing stream. The sample passage can be configured to provide a sample stream. The bifurcating passage can include a specified bifurcating flow resistance. The combining passage can be configured to create a combined stream from the focusing stream and the sample stream, where the focusing stream can direct the sample stream away from the upstream passage and toward the bifurcating passage. A first portion of the combined stream can be discharged through the bifurcating passage. The main discharge can be configured to discharge a second portion of the combined stream. The main discharge can include a main discharge resistance that is selectable to vary the main discharge resistance relative to the bifurcating flow resistance. 120-. (canceled)21. A method of operating microfluidic device to separate acinar cells from islet cells , the method comprising:providing a sample stream into a combining passage from an upstream sampling passage;providing a focusing stream from an upstream focusing passage to the combining passage to, together with the sample stream, form a combined stream, the focusing stream directing the sample stream away from the upstream passage and toward a bifurcating passage that defines a bifurcating flow resistance and that defines a passage width and depth;discharging a first portion of the combined stream through the bifurcating passage;discharging a second portion of the combined stream out of a main discharge, the main discharge including a main discharge resistance;varying the main discharge resistance using a main discharge multiplexer; andestablishing a threshold occlusion level to selectably accept particles in a specified deformability range for a specified size range in the bifurcating passage, the width or depth of the bifurcating passage, the main discharge ...

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Номер записи: 22
30-01-2020 дата публикации

FLUID PROCESSING MICRO-FEATURE DEVICES AND METHODS

Номер: US20200033239A1
Автор: Lee Ka Man, McPeak Daniel R.
Принадлежит:

The present description provides, in some embodiments, an apparatus for mixing a fluid in a circuit having an inlet channel defining a flow path for a fluid including particulate matter, a first reagent channel in fluid communication with the inlet channel and defining a first reagent flow path for a first reagent, the inlet channel and first reagent channel configured to shear the fluid entering the first reagent channel from the inlet channel at a first junction, a shearing channel in fluid communication with the inlet channel and first reagent channel at the first junction, and a diffusion channel in fluid communication with the shearing channel at a second junction, the sheared fluid collectable into the diffusion channel such that the fluid is compressed at least in part by the first reagent to have a thickness close to a diameter of the particulate matter in the fluid. 1. A method of mixing a fluid , comprising:injecting a fluid containing particulate matter into an inlet channel of a circuit;injecting a reagent into first and second reagent channels, the first and second reagent channels defining first and second reagent flow paths;shearing the fluid by the reagent at a first junction at which the fluid inlet channel and the first reagent channel merge into a shearing channel, a top wall of the inlet channel and a top wall of the shearing channel coplanar proximate the first junction; andcompressing the sheared fluid by the reagent at a second junction at which the shearing channel and second reagent channel merge into a diffusion channel,wherein the compressed fluid has a thickness less than a diameter of the particulate matter in the fluid, and the diffusion channel provides a length for at least a portion of the particulate matter extending into the reagent to react with the reagent.2. The method of claim 1 , wherein the compressed fluid has a thickness less than 8 μm.3. The method of claim 1 , wherein the fluid exhibits a flow rate (V) through the ...

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Номер записи: 23
08-02-2018 дата публикации

METHOD AND DEVICE FOR HIGH-THROUGHPUT SOLUTION EXCHANGE FOR CELL AND PARTICLE SUSPENSIONS

Номер: US20180036732A1
Автор: Di Carlo Dino, Gossett Daniel R., Tse Henry T.K.
Принадлежит: The Regents of the University of California

A method of exchanging fluids with suspended particles includes providing a microfluidic device with a first inlet channel operatively coupled to a source of particles and a second inlet channel operatively coupled to an exchange fluid. A transfer channel is connected at a proximal end to the first inlet channel and the second inlet channel. First and second outlet channels are connected to a distal end of the transfer channel. The source of particles is flowed at a first flow rate into the first inlet channel while the exchange fluid is flowed at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5. Particles are collected in one of the first and second outlet channels while fluid substantially free of particles is collected in the other of the first and second outlet channels. 133-. (canceled)34. A method of migrating particles suspended in a fluid comprising:providing a microfluidic device comprising a first inlet channel operatively coupled to a fluid having particles suspended therein; a second inlet channel operatively coupled to a buffer solution; a transfer channel having a proximal end and a distal end, the proximal end of the transfer channel connected to the first inlet channel and the second inlet channel; and first and second outlet channels connected to a distal end of the transfer channel;flowing the fluid containing the particles at a first flow rate into the first inlet channel;{'sub': eq', 'eq', 'eq, 'flowing the buffer solution at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5, wherein the particles migrate toward an equilibrium position (X) located within the transfer channel, the equilibrium position (X) contained within the buffer solution, wherein inertial lift forces in the transfer channel direct the particles toward the equilibrium position (X);'}collecting the particles that have ...

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Номер записи: 24
08-02-2018 дата публикации

FLOW CYTOMETRY USING HYDRODYNAMICALLY PLANAR FLOW

Номер: US20180038783A1
Автор: Kayou Tsukasa, Masuda Yuji, Robinson J. Paul, YAMAMOTO Masanobu
Принадлежит:

According to various aspects, a flow system for transporting microparticulate samples in a hydrodynamically planar flow in a selected flow direction includes a flow chamber extending in the flow direction, having first and second apertures on opposed surfaces of the flow chamber. A sheath-fluid channel has first and second branches to carry the sheath fluid into the flow chamber through the first aperture and having orientations separated by less than about 15° at the first aperture; and third and fourth branches to carry the sheath fluid through the second aperture and having orientations separated by less than about 15° at the second aperture. In some examples, guide channels extend from the apertures substantially perpendicular to the flow chamber at the apertures, and sheath-fluid channel supply sheath fluid to the guide channels. Flow systems can be used in image flow cytometers for observing microparticulate samples, e.g., using scanning irradiation. 1. A flow system for transporting microparticulate samples in a hydrodynamically planar flow in a flow direction , the flow system comprising:a flow chamber extending in the flow direction, the flow chamber including first and second apertures through opposed walls of the flow chamber, the flow chamber configured to receive a carrier fluid including the microparticulate samples at a location upstream of the first and second apertures; and first and second branches, each of the first and second branches configured to carry the sheath fluid into the flow chamber through the first aperture and having a respective longitudinal axes, wherein the respective longitudinal axes have a relative angle between them of less than about 15° at the first aperture; and', 'third and fourth branches, each of the third and fourth branches configured to carry the sheath fluid into the flow chamber through the second aperture and having respective longitudinal axes, wherein the respective longitudinal axes have a relative angle between ...

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Номер записи: 25
18-02-2021 дата публикации

Multichannel isoelectric focusing devices and high voltage power supplies

Номер: US20210046480A1
Автор: Eric Gwerder, Erik Gentalen, Scott Mack
Принадлежит: Intabio LLC

Methods, devices, and systems for performing a plurality of isoelectric focusing reactions in parallel are described. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.

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Номер записи: 26
18-02-2021 дата публикации

Microfluidic Device

Номер: US20210046481A1
Автор: Frank Tino
Принадлежит:

A microfluidic device includes a chamber, on two sides of which lying opposite each other in a first direction, a respective first distributor is provided in order to produce a laminar flow in the first direction. Each of the first distributors has at least one branching point, at which a channel is divided into at least two channels. The at least one branching point of the first distributor is arranged in such a way that a first connection channel is connected to a plurality of first connection points of the chamber by means of the first distributor. 1. A microfluidic device comprising:a chamber comprising a first side and a second side, which are opposite one another in a first direction; anda respective first distributor located on each of the first and second sides, the respective first distributors configured to generate a laminar flow in the first direction,wherein each of the respective first distributors includes at least one first branching site at which a channel divides into at least two channels, andwherein the at least one first branching site of each respective first distributor is arranged such that a first connection channel is connected to a plurality of first connection points of the chamber via the respective first distributor.2. The microfluidic device as claimed in claim 1 , wherein:the chamber further comprises a third side and a fourth side, which are opposite one another in a second direction that is different from the first direction;the microfluidic device further comprises a respective second distributor arranged on each of the third and fourth sides and configured to generate a laminar flow in the second direction;each of the second distributors has respectively at least one second branching site at which a channel divides into at least two channels; andthe at least one second branching site of each respective second distributor is arranged such that a second connection channel is connected to a plurality of second connection points of ...

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Номер записи: 27
18-02-2016 дата публикации

Microchip and particulate analyzing device

Номер: US20160045916A1
Автор: Masaya Kakuta, Shoji Akiyama, Takeshi Yamasaki, Tatsumi Ito
Принадлежит: Sony Corp

A microchip is provided, which includes a substrate including a fluid channel structure. The fluid channel structure includes a first fluid introduction channel and a second fluid introduction channel configured to meet so as to allow merging of a first fluid introduced from the first fluid introduction channel and a second fluid introduced from the second fluid introduction channel. A tapered portion is configured to be positioned after merging the first fluid and the second fluid so as to suppress a spiral flow field generated after the merging.

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Номер записи: 28
06-02-2020 дата публикации

Microfluidic System and Method for Real-Time Measurement of Antibody-Antigen Binding and Analyte Detection

Номер: US20200041501A1
Автор: KONRY Tania, Yarmush Martin L.
Принадлежит:

Microfluidic devices for use with reagents bound to microspheres for determination of the concentration of an analyte in a liquid sample are provided. The devices include two sequential mixing channels that promote rapid binding of microsphere-bound reagents with reagents in solution and a means for detecting labeled microsphere-bound reaction products. Also provided are methods for using the devices with microsphere-bound reagents to determine the concentration of an analyte in a liquid sample and to measure the binding affinity of antibody for an antigen. 1. A microfluidic device for continuous flow optical detection of an analyte in a sample , the device comprising:(a) first and second inlets;(b) a first microscale laminar flow channel fluidically connected to the first and second inlets such that liquids entering from the first and second inlets flow in a laminar manner through said first laminar flow channel;(c) a first microscale mixing channel fluidically connected to the first laminar flow channel such that liquid entering the first mixing channel from the first laminar flow channel is converted from laminar flow to non-laminar flow in said first mixing channel;(d) a second microscale laminar flow channel fluidically connected to the first mixing channel;(e) a third inlet fluidically connected to the second laminar flow channel such that liquids entering the second laminar flow channel from the third inlet and the first mixing channel flow in a laminar manner in said second laminar flow channel;(f) a second microscale mixing channel fluidically connected to the second laminar flow channel such that liquid entering the second mixing channel from the second laminar flow channel is converted from laminar flow to non-laminar flow in said second mixing channel;(g) an outlet fluidically connected to the second mixing channel; and (1) comprises at least a portion of the second mixing channel; and/or', '(2) is disposed between the second mixing channel and the ...

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Номер записи: 29
15-02-2018 дата публикации

PARTICLE SEPARATION AND CONCENTRATION USING SPIRAL INERTIAL FILTRATION

Номер: US20180045626A1
Автор: Burke Jeffrey M., White Ian M.
Принадлежит: University of Maryland

A spiral inertial filtration device is capable of high-throughput (1 mL/min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude. Large fractions of sample fluid are removed from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the device for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were achieved by maintaining either average fluid velocity or Dean number throughout the entire length of the channel during the incremental removal of sample fluid. Also provided is the ability to simultaneously separate more than one particle from the same sample. 1. A method of filtering and/or concentrating particles of a fluid containing particles of one or more particle sizes , said method comprising:moving the fluid in a spiral path under conditions that cause differential migration of particles within the moving fluid into unique equilibrium positions according to the size of the particle thereby forming one or more particle streams and a particle-free region within the moving fluid;drawing at least a portion of the fluid from the particle-free region of the moving fluid to increase the concentration of particles within the remainder of the moving fluid; andcollecting particles from one or more of the particle streams.2. The method of claim 1 , wherein the collecting step is performed at the end of the spiral path.3. The method of claim 1 , wherein the particles comprise one or more components of interest within a sample fluid.4. The method of claim 3 , further comprising increasing the concentration of each component of interest.5. The method of claim 1 , wherein the particles of each of the one or more ...

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Номер записи: 30
15-02-2018 дата публикации

PARTICLE SORTING APPARATUS, PARTICLE SORTING METHOD, AND NON-TRANSITORY COMPUTER-READABLE STORAGE MEDIUM STORING PROGRAM

Номер: US20180045638A1
Автор: HATAMOTO KOUHEI, KASAI HIROTO, Kato Takayuki, MIYATA Takashi, OTSUKA FUMITAKA
Принадлежит:

The present disclosure provides a particle sorting apparatus, a particle sorting method, and a non-transitory computer-readable storage medium storing program that enable sorting object particles to be sorted with high precision, even when the sorting object particles are large. In the particle sorting apparatus, a charging unit that applies charges to at least a part of liquid droplets ejected from an orifice to generate a fluid stream and a charging control unit that adjusts a charge application end time in the charging unit according to sizes of particles included in the liquid droplets are provided. 1. A particle sorting apparatus , comprising:a charging unit configured to apply charges to at least a portion of a plurality of liquid droplets ejected from an orifice; and wherein the mode is selected from one of a first mode or a second mode, based on a size of particles included in the plurality of liquid droplets, and', 'wherein at least one of the charge application end time or a charge application duration of the first mode is different from the second mode., 'a charging control unit configured to adjust a charge application end time for the application of the charges, based on a mode of the particle sorting apparatus,'}2. The particle sorting apparatus according to claim 1 , wherein the first mode is a normal mode and the second mode is a large diameter particle mode.3. The particle sorting apparatus according to claim 1 , wherein the charging control unit is further configured to change a start time for the application of the charges based on sizes of the plurality of particles.4. The particle sorting apparatus according to claim 1 , wherein the charging control unit is further configured to change the charge application duration based on sizes of the plurality of particles.5. The particle sorting apparatus according to claim 1 ,wherein the orifice is present in an exchangeable microchip, andwherein the charging unit includes a charging electrode to contact ...

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Номер записи: 31
14-02-2019 дата публикации

ARRANGEMENT FOR INDIVIDUALIZED PATIENT BLOOD ANALYSIS

Номер: US20190049359A1
Автор: Bauer Michael, Henkel Thomas, NEUGEBAUER Ute, POPP Juergen
Принадлежит:

An arrangement for the individualized in-vitro patient blood analysis includes a holography module, a Raman spectroscopy module, a biomarker module and a flow controller which are connected in a data and information transmitting manner to a central control and computer unit, which has an information transmitting connection to a database, wherein the modules are fluidically connected to a common blood sample supply and supplies for fluid other than blood via the flow controller. 111.-. (canceled)12. An arrangement for the individualized in-vitro patient blood analysis comprising a holography module , a Raman spectroscopy module , a biomarker module and an integrated flow controller which have a data and information transmitting connection to a central control and computer unit which has an information transmitting connection to a database , wherein;the holography module comprises a holography sample preparation cartridge and a holography sample measurement unit which are microfluidically connected to each other;the Raman spectroscopy module comprises a Raman spectroscopy sample preparation cartridge and a Raman spectroscopy sample measurement unit which are microfluidically connected to each other,the biomarker module comprises a biomarker sample preparation cartridge and biomarker sample measurement unit which are microfluidically connected to each other;the holography, Raman spectroscopy and biomarker modules are fluidically connected to a common blood sample supply and supplies for fluid other than blood via the flow controller,the holography sample preparation cartridge comprises microstructured microfluidic lysis channels or dielectrophoresis channels provided with microelectrodes, the channels of the holography sample preparation cartridge being microfluidically connected to the flow controller, and the holography sample measurement unit includes a lensless inline reflected-light microscope with a coherent illumination source and a detector array,the Raman ...

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Номер записи: 32
25-02-2021 дата публикации

MICROCHIP IMMUNOASSAY DEVICE HAVING PRECISE INCUBATION TIME CONTROL AND SIGNAL SCALING AND RELATED METHODS

Номер: US20210055284A1
Автор: Ding Zhong
Принадлежит:

A lateral flow (immunoassay) device retains at least one assay chip inside a solid frame. Each assay chip includes a sample zone, a conjugate zone, capture zone(s) and a waste zone. The sample and waste zones each include a hydrophilic pad sitting above the chip. The frame is hydrophobic, having a fluid metering window and optional air vent in the top and a scan window provided at the bottom. A sample with analyte is dispensed with the volume of sample such that fluid flow stops when solution with dissolved conjugate reaches a designated location in the chip, and at least a portion of the waste zone is still dry. A second fluid is subsequently added to wash off unbound conjugate in the capture zone after the device with first fluid has been incubated for an assay specified time period. During incubation and after wash, multiple optical scans are performed to obtain conjugate signal profiles along the chip. The features of the signal are used to define an assay signal read location. The bound conjugate signal after wash is then scaled by the total conjugate signal prior to wash at the read area. This scaled signal is defined as assay response. A single device can perform single test for one sample as well as multiple tests for a single or multiple samples. 1. An immunoassay device comprising:a frame defining an interior;an assay chip disposed within the interior;a sample zone including a sample pad adjacent one end of the assay chip, in which the sample zone that is configured to receive a first sample fluid having at least one analyte of interest, the assay chip being made from a porous hydrophilic material that enables fluid transport;at least one conjugate zone downstream of the sample zone, the conjugate zone comprising a conjugate material;at least one capture zone downstream of the at least one conjugate zone having at least one capture antibody; andat least one waste zone downstream of the at least one capture zone, wherein the assay chip comprises a ...

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Номер записи: 33
23-02-2017 дата публикации

METHOD AND DEVICE FOR HIGH-THROUGHPUT SOLUTION EXCHANGE FOR CELL AND PARTICLE SUSPENSIONS

Номер: US20170052107A1
Автор: Di Carlo Dino, Gossett Daniel R., Tse Henry T.K.
Принадлежит: The Regents of the University of California

A method of exchanging fluids with suspended particles includes providing a microfluidic device with a first inlet channel operatively coupled to a source of particles and a second inlet channel operatively coupled to an exchange fluid. A transfer channel is connected at a proximal end to the first inlet channel and the second inlet channel. First and second outlet channels are connected to a distal end of the transfer channel. The source of particles is flowed at a first flow rate into the first inlet channel while the exchange fluid is flowed at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5. Particles are collected in one of the first and second outlet channels while fluid substantially free of particles is collected in the other of the first and second outlet channels. 133-. (canceled)34. A microfluidic system for solution exchange comprising:a first inlet channel operatively coupled to a source of particles suspended in a fluid;a second inlet channel operatively coupled to an exchange fluid;a linear and longitudinally symmetrical transfer channel having a proximal end and a distal end, the proximal end of the transfer channel connected to the first inlet channel and the second inlet channel;a plurality of outlet channels connected to a distal end of the transfer channel; and;{'sub': 'eq', 'a first pump configured to pump the source of particles suspended in a fluid at a first flow rate and a second pump configured to pump the exchange fluid at a second flow rate, wherein the ratio of the second flow rate to the first flow rate is at least 1.5 and wherein the exchange fluid occupies more than half of the transfer channel and wherein inertial lift forces in the transfer channel direct the particles from the source of particles to an equilibrium position (X) located within a center of the transfer channel and within the exchange fluid.'}35. The microfluidic system of claim 34 , ...

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Номер записи: 34
23-02-2017 дата публикации

FLUIDIC ANALYSIS AND SEPARATION

Номер: US20170052147A1
Автор: Herling Therese, KNOWLES Tuomas, Mueller Thomas
Принадлежит: Cambridge Enterprise Limited

A method for analyzing a component is provided. The method includes the steps of: (iii) providing the electrophoretic or thermophoretic movement of the component into a second fluid flow; (iv) diverting a part of a first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, wherein the diverted part is a third fluid flow which includes, the component; (v) contacting the third fluid flow with a fourth fluid flow, such as to form a laminar flow; (vi) providing the diffusion of the component into the fourth fluid flows. 1. A method for analyzing a component , the method comprising the steps of:(i) providing the component in a first fluid flow; and(ii) contacting the fluid flow with a second fluid flow, such as to generate a laminar flow,(iii) providing electrophoretic or thermophoretic movement of the component into the second fluid flow;(iv) diverting a part of a the first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, wherein the diverted part is a third fluid flow which comprises the component;(v) contacting the third fluid flow with a fourth fluid flow, such as to generate said laminar flow;(vi) providing diffusion of the component into the fourth fluid flows,(vii) optionally diverting a part of the third fluid flow, a part of the fourth fluid flow, or parts of the third fluid flow and fourth fluid flow; wherein the diverted part is a fifth fluid flow which comprises the component; and subsequently(viii) labeling the component in a diverted flow, such as the third fluid flow or the fifth fluid flow.224.-. (canceled)25. The method of claim 1 , further comprising the subsequent step of:(ix) contacting the fifth fluid flow with a sixth fluid flow, such as to form a laminar flow;(x) providing a distribution of the component across contacting fifth and sixth fluid flows, such as laminar fluid flows.26. The method of claim 25 , further comprising the subsequent ...

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Номер записи: 35
21-02-2019 дата публикации

APPARATUS FOR SEPARATING MICRO-PARTICLES USING TRIANGULAR MICROCHANNEL

Номер: US20190054464A1
Автор: Kim Jeong-Ah, Lee Wonhee
Принадлежит:

A micro-particle separation apparatus includes a triangular microchannel of which a cross-section is formed in the shape of a triangle and through which a fluid including a plurality of particles flows by a predetermined length; and an outlet that separates particles that have been arranged at different focusing positions in the triangular microchannel, and outputs the separated particles. The triangular microchannel has different focusing positions depending on particle size. 1. A micro-particle separation apparatus comprising:a triangular microchannel of which a cross-section is formed in the shape of a triangle and through which a fluid including particles with a plurality of sizes flows by a predetermined length; andan outlet that separates the particles that have been arranged at different focusing positions in the triangular microchannel, and outputs the separated particles ,wherein the triangular microchannel makes different focusing positions depending on particle size.2. The micro-particle separation apparatus of claim 1 , wherein claim 1 , in the triangular microchannel claim 1 , focusing positions shift along two side walls from the top corner of the cross-section of the channel as the particle size is decreased.3. The micro-particle separation apparatus of claim 2 , wherein claim 2 , in the triangular microchannel claim 2 , the particles are focused above and below the center of the cross-section of the channel depending on particle size or focused near each side wall of the triangular microchannel.4. The micro-particle separation apparatus of claim 1 , wherein claim 1 , in the triangular microchannel claim 1 , focusing position of particles with a specific size shifts along two side walls from the top corner of the cross-section of the channel as a Reynolds number is increased.5. The micro-particle separation apparatus of claim 4 , wherein the Reynolds number is changed by adjusting at least one of velocity of the fluid having flowed into the triangular ...

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Номер записи: 36
20-02-2020 дата публикации

OPTOFLUIDIC DEVICE AND METHOD FOR DETECTING CIRCULATING TUMOUR CELLS

Номер: US20200055048A1
Автор: Alvarez-Puebla Ramon Angel, Diaz Francisco, Fernandez Eduardo Garcia-Rico, Garcia Algar Manuel, Massons Jaume, Nazarenus Moritz Julian, Pedrol Eric
Принадлежит:

The present invention relates to a microfluidic device for the detection of circulating tumour cells (CTCs) in a fluid sample. The invention also relates to an in vitro method for the detection and/or quantification of circulating tumour cells (CTCs) in a fluid sample. Further, the invention relates to in vitro methods for (a) diagnosing a tumour and/or metastasis in a subject, and (b) determining the prognosis of a subject suffering from a tumour and/or metastasis. Finally, the invention refers to a kit comprising (a) a microfluidic device according to the first aspect of the invention, and (b) labelled probes targeting a first CTCs surface marker and, optionally, at least a second CTCs surface marker. 117.-. (canceled)18. A microfluidic device for the detection of circulating tumour cells (CTCs) in a fluid sample comprising: the hydrodynamic focusing element comprises at least three inlets, a first central inlet for the entrance of a labelled fluid sample, and a second and third inlets for the entrance of a focusing fluid for flow focusing of the labelled fluid sample in a first plane, wherein the second and third inlets are situated at opposite sides of the first central inlet, and optionally, a fourth and fifth inlets for the entrance of a focusing fluid for flow focusing of the labelled fluid sample in a second plane perpendicular to the first plane, wherein the fourth and fifth inlets are situated at opposite sides of the first central inlet, all the inlets converging into a single microfluidic channel; and wherein', 'the inertial focusing element comprises a functional microchannel selected from the group consisting of a straight channel, a straight channel with pillar arrays, a straight channel with expansion-contraction arrays, a spiral channel, a serpentine channel, and combinations thereof;, '(a) a cell focusing element selected from a hydrodynamic focusing element and an inertial focusing element, wherein'}(b) a single microfluidic channel comprising at ...

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Номер записи: 37
05-03-2015 дата публикации

Multidimensional hydrodynamic focusing chamber

Номер: US20150064694A1
Автор: Amir M. Sadri, Nenad Kircanski, Tal Rosenzweig
Принадлежит: Bio Rad Laboratories Inc

Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads. The systems may include a shaped focusing chamber and/or a branched diverting mechanism.

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Номер записи: 38
27-02-2020 дата публикации

DEVICES AND METHODS FOR NUCLEIC ACID IDENTIFICATION

Номер: US20200061617A1
Автор: Patil Vishal A.
Принадлежит:

Provided herein are devices and methods for obtaining nucleotide sequence information from nucleic acid and nucleic acid samples. The device includes a microfluidic channel that aids in manipulating a sample as the sample flows through various regions of the channel. The devices and methods are sensitive enough to detect signal from and thus interrogate single nucleic acids on an individual basis rather than as a bulk population. 1. A device for manipulating a polymer in a fluid sample , the device comprising:a sample fluid inlet;a channel in fluid communication with the sample fluid inlet, the channel having a focusing region, a cutting region, a relaxation region, and a detection region,the focusing region including a pair of supplementary inlets in fluid communication with the channel, the pair of supplementary inlets opposing one another on either side of the channel,the cutting region having a converging width shape.2. The device of claim 1 , wherein the relaxation region has a first portion having a converging width shape and a second portion having a diverging width shape.3. The device of claim 2 , wherein the converging width shape of the first portion of the relaxation region has a sharper rate of convergence than the converging width shape of the cutting region.4. The device of claim 1 , wherein at least a portion of the focusing region has a converging width shape.5. (canceled)6. The device of claim 4 , wherein the converging width shape of the focusing region has a sharper rate of convergence than the converging width shape of the cutting region.7. The device of claim 2 , wherein at least a portion of the focusing region has a converging width shape claim 2 , and wherein the converging width shape of the first portion of the relaxation region is a different shape than the converging width shape of the focusing region.8. The device of claim 1 , wherein the supplementary inlets are connected to the channel at the focusing region of the channel.9. (canceled ...

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Номер записи: 39
28-02-2019 дата публикации

SAMPLE ISOLATION KIT, SAMPLE ISOLATION DEVICE

Номер: US20190064049A1
Автор: Katsumoto Yoichi, Matsumoto Masahiro, TAKAHASHI Kazuya
Принадлежит: SONY CORPORATION

According to some aspects, a sample isolation kit is provided. The sample isolation kit includes a housing configured to detachably couple to a sample fluid channel of a microchip and provide a sample to the microchip. The housing and the microchip are coupled using a hermetic seal. The sample isolation kit further includes a storage housing configured to detachably couple to an isolation channel of the microchip and receive a target biological sample isolated from the sample by the microchip. The storage housing and the microchip are coupled using a hermetic seal. 1. A sample isolation kit comprising:a housing configured to detachably couple to a sample fluid channel of a microchip and provide a sample to the microchip, wherein the housing and the microchip are coupled using a hermetic seal; anda storage housing configured to detachably couple to an isolation channel of the microchip and receive a target biological sample isolated from the sample by the microchip, wherein the storage housing and the microchip are coupled using a hermetic seal.2. The sample isolation kit of claim 1 , further comprising:a sheath container configured to couple to at least one sheath fluid channel of the microchip and provide sheath fluid to the microchip.3. The sample isolation kit of claim 2 , further comprising:a pressure regulation device coupled to the sheath container and configured to regulate the pressure of the sheath container.4. The sample isolation kit of claim 3 , wherein the pressure regulation device is coupled to the housing and is configured to regulate the pressure of the housing.5. The sample isolation kit of claim 3 , wherein the pressure regulation device is coupled to the storage housing and is further configured to generate a negative pressure at the storage housing.6. The sample isolation kit of claim 1 , further comprising:a labeling unit configured to label the sample with the at least one fluorescent dye.7. The sample isolation kit of claim 6 , wherein the ...

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Номер записи: 40
09-03-2017 дата публикации

MULTILAYER HYDRODYNAMIC SHEATH FLOW STRUCTURE

Номер: US20170066605A1
Автор: Bunner Bernard, Deshpande Manish, Gilbert John R.
Принадлежит:

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip. 114-. (canceled)15. A microfluidic system comprising:a substrate;a sample fluid inlet provided on a surface of the substrate;a sample fluid channel configured to receive sample fluid from the sample fluid inlet;a first sheath fluid inlet;first and second sheath fluid branch channels configured to receive sheath fluid from the first sheath fluid inlet;a second sheath fluid inlet;third and fourth sheath fluid branch channels configured to receive sheath fluid from the second sheath fluid inlet;a first focusing region configured to receive sample fluid from the sample fluid channel and sheath fluid from the first and second sheath fluid branch channels; anda second focusing region downstream of the first focusing region and configured to receive sample fluid and sheath fluid from the first focusing region and to receive sheath fluid from the third and fourth sheath fluid branch channels.16. The system of claim 15 , wherein the first focusing region is configured to symmetrically receive sheath fluid from the first and second sheath fluid branch channels relative to a centerline of the sample fluid flowing in the first focusing region.17. The system of claim 15 , wherein the second focusing region is configured to symmetrically receive sheath fluid ...

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Номер записи: 41
11-03-2021 дата публикации

Systems and Methods for Electronic Surface Antigen Expression Analysis Using Magnetophoresis

Номер: US20210069713A1
Автор: Civelekoglu Ozgun, SARIOGLU Ali Fatih
Принадлежит:

Embodiments of the present disclosure relate generally to systems and methods for sorting and analyzing cells and, more particularly, to systems and methods for sorting and analyzing cells using magnetophoresis in a microfluidic platform. Some embodiments of a microfluidic device comprise an inlet for receiving a plurality of magnetically-labeled cells, a flow chamber, a magnet positioned alongside the flow chamber, and a plurality of bins having a sensor for detecting the magnetically-labeled cells. In some embodiments, the magnetic flux of the magnet causes the magnetically-labeled cells to be deflected to a particular bin. The sensors of each bin can be used to calculate the surface antigen expression and/or size of the cells within a sample of magnetically-labeled cells. 1. A microfluidic device comprising:a first inlet configured to receive a first fluid comprising a plurality of magnetically-labeled cells;a first flow chamber having a first end and a second end, the first end in fluidic communication with the first inlet;a plurality of bins, each bin having a first end and a second end, the first end of each bin in fluidic communication with the second end of the first flow chamber;a first magnet disposed adjacent to the first flow chamber, the first magnet configured to attract the magnetically-labeled cells towards a bin of the plurality of bins; anda plurality of sensors, each sensor disposed at the second end of a corresponding bin of the plurality of bins, each sensor configured to produce a unique signal in response to a cell of the plurality of magnetically-labeled cells passing through the bin corresponding to the sensor.2. The microfluidic device of further comprising:a second flow chamber disposed between the first inlet and the first flow chamber, the second flow chamber comprising a first outlet and a second outlet, the first outlet exiting into the first flow chamber, and the second outlet not exiting into the first flow chamber;a second magnet ...

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Номер записи: 42
24-03-2022 дата публикации

Microfluidic chip and device

Номер: US20220088604A1
Автор: Ching-Chih Chang, Hui-Min Yu, Jui-Lin Chen, Wei-Feng Fang
Принадлежит: Micareo Taiwan Co ltd

A microfluidic device and a microfluidic chip are provided. The microfluidic device includes the microfluidic chip, a pouring element, a flow adjustment element and a processor. The microfluidic chip includes a sorting assembly, a sample outlet channel, a pouring channel, a collection channel and a waste channel. The sorting assembly includes a sample inlet channel and a sorting chamber. The pouring element is connected to the pouring channel. The flow adjustment element is connected to a distal end of the sample outlet channel. The processor is configured to control the pouring element to pour a guiding fluid into the pouring channel entering the sample outlet channel and control the flow adjustment element to adjust a flow resistance of a drain section of the sample outlet channel.

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Номер записи: 43
24-03-2022 дата публикации

Isoelectric focusing devices and fixtures

Номер: US20220088605A1
Автор: Don Wesley Arnold, Eric Gwerder, Erik Gentalen, Luc Bousse, Scott Mack
Принадлежит: Intabio LLC

Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.

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Номер записи: 44
07-03-2019 дата публикации

SYSTEMS, DEVICES, AND METHODS FOR ISOTACHOPHORESIS

Номер: US20190071661A1
Автор: ARIN Sean, Hiddessen Amy L., HOVERTER Nathan P., Lee Andrew, Marshall Lewis A., Mok Janine, MUNSON Matthew S., QU Yatian, Rose Klint A., Santiago Juan G.
Принадлежит:

The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing. 1. A fluidic device comprising an isotachophoresis (ITP) circuit comprising:a first channel comprising first and second capillary barriers that are spaced apart;a first loading reservoir in fluid communication with said first channel via a first aperture in said first channel, wherein said first aperture is positioned between said first and second capillary barriers to permit a liquid entering said first channel via said first aperture to flow in one direction along said first channel and arrest at said first capillary barrier and to flow in another direction along said first channel and arrest at said second capillary barrier.2. The fluidic device of claim 1 , wherein said liquid entering said first channel via said aperture flows along a path to said first or second capillary barrier that is longer than a width of said first channel.3. The fluidic device of claim 1 , wherein said liquid entering said first channel via said aperture flows such that a meniscus of said first liquid arrests at said first capillary barrier or at said second capillary barrier claim 1 , or at both said first and said second capillary barrier.4. The fluidic device of claim 1 , wherein said first capillary barrier is configured and arranged to be breached by a liquid when a first burst pressure is applied to said one or more branched fluidic circuits and said second capillary barrier is configured and arranged to be breached by said liquid when a second burst pressure is applied to said one or more ...

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Номер записи: 45
05-03-2020 дата публикации

Microfluidic device with array of chambers for encoding detectable information

Номер: US20200070170A1
Автор: Cristina G. M. Mercandetti, Emmanuel Delamarche, Onur Gökçe
Принадлежит: International Business Machines Corp

Embodiments of the invention are directed to a microfluidic device. The device comprises a flow path structure that includes an inlet microchannel and chambers. The flow path structure is configured as an arborescence extending from the inlet microchannel to the chambers. Thus, liquid introduced in said inlet microchannel can potentially enter the chambers via respective flow paths to remain essentially confined in the chambers, in operation. The device further comprises substances in selected ones of the chambers. That is, a subset of the chambers is loaded with substances adapted for interacting with liquid to yield a detectable change in a property of the liquid and/or the substance in each of the chambers of said subset, in operation. The invention is further directed to related devices, and methods of operation and conditioning.

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Номер записи: 46
15-03-2018 дата публикации

UNITARY CARTRIDGE FOR PARTICLE PROCESSING

Номер: US20180074060A1
Автор: Beaupre Derek, Deshpande Manish, Gilbert John R., Lewis Hugh, Trikha Jaishree
Принадлежит:

A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles. 1. A particle sorting system comprising: a particle sorting component configured to receive a stream of particles and to individually sort on a particle-by-particle basis the stream of particles into selected particles having a predetermined characteristic and unselected particles not having the predetermined characteristic;', 'a particle source upstream of and in fluid communication with the particle sorting component for providing particles to be sorted to the particle sorting component;', 'a first chamber downstream of and in fluid communication with the particle sorting component for collecting the selected particles;', 'a second chamber downstream of the particle sorting component for collecting unselected particles; and', 'one or more sealing elements configured to completely seal the cartridge during a particle sorting operation so as to prevent liquid transfer to and from the interior of the cartridge., 'a cartridge including2. The particle sorting system of claim 1 , wherein the particle source chamber is configured to provide particles to be sorted to the particle sorting component when an external source of pressure is applied to the particle source chamber.3. The particle sorting system of claim 1 , further comprising:a sealable loading port formed in the cartridge for providing particles to the particle source chamber; andan extraction port formed in the cartridge for removing selected particles from the first chamber.4. The particle sorting system of claim 1 , further ...

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Номер записи: 47
16-03-2017 дата публикации

Microfluidic System and Method for Real-Time Measurement of Antibody-Antigen Binding and Analyte Detection

Номер: US20170074870A1
Автор: KONRY Tania, Yarmush Martin L.
Принадлежит:

Microfluidic devices for use with reagents bound to microspheres for determination of the concentration of an analyte in a liquid sample are provided. The devices include two sequential mixing channels that promote rapid binding of microsphere-bound reagents with reagents in solution and a means for detecting labeled microsphere-bound reaction products. Also provided are methods for using the devices with microsphere-bound reagents to determine the concentration of an analyte in a liquid sample and to measure the binding affinity of antibody for an antigen. 1. A microfluidic device for continuous flow optical detection of an analyte in a sample , the device comprising:(a) first and second inlets;(b) a first microscale laminar flow channel fluidically connected to the first and second inlets such that liquids entering from the first and second inlets flow in a laminar manner through said first laminar flow channel;(c) a first microscale mixing channel fluidically connected to the first laminar flow channel such that liquid entering the first mixing channel from the first laminar flow channel is converted from laminar flow to non-laminar flow in said first mixing channel;(d) a second microscale laminar flow channel fluidically connected to the first mixing channel;(e) a third inlet fluidically connected to the second laminar flow channel such that liquids entering the second laminar flow channel from the third inlet and the first mixing channel flow in a laminar manner in said second laminar flow channel;(f) a second microscale mixing channel fluidically connected to the second laminar flow channel such that liquid entering the second mixing channel from the second laminar flow channel is converted from laminar flow to non-laminar flow in said second mixing channel;(g) an outlet fluidically connected to the second mixing channel; and (1) comprises at least a portion of the second mixing channel; and/or', '(2) is disposed between the second mixing channel and the ...

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Номер записи: 48
05-03-2020 дата публикации

SYSTEM FOR MICROBIAL SPECIES DETECTION, QUANTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY IDENTIFICATION

Номер: US20200071740A1
Автор: FINCH Michael D., FONG Kee Onn, KOTIAN Saurabh, Lindborg Beth A., MAHAJAN Advitiya, Munshi Cyrus B.
Принадлежит: Urinary Technologies, Inc.

Several microfluidic chips are used to significantly accelerate the time to identify and quantify microbes in a biological sample and test them for antibiotic resistance, particularly for urinary tract infections. A first microfluidic chip uses antibody or similar probes to identify and quantify any microbes present. The same or a similar chip uses antibody or similar probes to identify microbes with DNA or RNA known to indicate antibiotic resistance. Another microfluidic chip tests for antibiotic susceptibility of any microbes by growing them in very small wells in the presence of antibiotics, reducing the time required for such testing by as much as 95%. Another microfluidic chip runs traditional urinalysis or similar tests. 1. An apparatus for analyzing a biological sample , comprising: i. a plurality of microfluidic branches, an internal surface of each branch comprising a substance to which microbes will adhere;', 'ii. inlets connected to each said microfluidic branch for receiving the biological sample and delivering it to said microfluidic branches,', 'iii. a plurality of probe reservoirs, each said reservoir containing a probe supply and connected to a subset of said microfluidic branches to deliver said probe to said subset of microfluidic branches, and', 'iv. outlets connected to said microfluidic branches for removing fluid therefrom;, 'a. a microfluidic microbe detection chip (MDC), the MDC comprisingb. a sample transfer mechanism for receiving the biological sample and delivering it to said inlets of said MDC;c. at least one MDC solution reservoir, each said reservoir containing a solution and connected to said microfluidic branches to deliver said solution to said microfluidic branches;d. at least one MDC sensor adjacent to said microfluidic branches to measure the presence of said probes therein;e. a programmed control system for operating the apparatus, receiving measurements from said at least one MDC sensor and reporting the results.2. The ...

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Номер записи: 49
23-03-2017 дата публикации

Device for the Separation of Particles Using a Bulk Acoustic Wave Field

Номер: US20170080423A1
Автор: Dauson Erin R., Gregory Kelvin B., Greve David W., Healy Gregory P., Oppenheim Irving J.
Принадлежит: CARNEGIE MELLON UNIVERSITY, a Pennsylvania Non-Profit Corporation

The disclosure describes a prism containing a microfluidic channel. By coupling bulk acoustic wave generators to opposing sides of the prism, a standing bulk acoustic wave field can be excited in the prism and in the microfluidic channel. Because the microfluidic channel is titled with respect to the nodes of the bulk acoustic wave field, the prism microfluidic channel device can be used to separate microparticles and biological cells by size, compressibility, density, shape, or mass distribution. This technology enables high throughput cell sorting for biotechnology applications such as cancer cell detection. 1. A particle separation device comprising:a prism;a microchannel disposed within an interior portion of the prism;a first bulk acoustic wave generator acoustically coupled to a first side of the prism; wherein the first side is opposite the second side', 'wherein the bulk acoustic wave generators create at least one standing wave within the microchannel,', 'wherein the at least one standing wave is not parallel to a side of the microchannel., 'a second bulk acoustic wave generator acoustically coupled to a second side of the prism,'}2. The separation device of claim 1 , wherein the prism comprises poly (methyl methacrylate).3. The separation device of claim 1 , wherein the prism comprises a rigid polymer.4. The separation of device of claim 1 , wherein the prism comprises:a first part having a groove disposed on the surface of the first part; 'wherein the surface of the first part is mated to the surface of the second part forming the microchannel.', 'a second part substantially matching the shape of the first part and having a surface,'}5. The separation device of claim 4 , further comprising:an alignment mechanism on the surface of the first part;a complementary alignment mechanism on the surface of the second part, wherein the alignment mechanism of the first part engages the alignment mechanism of the second part when the first part is mated to the second ...

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Номер записи: 50
23-03-2017 дата публикации

SYSTEMS AND METHODS FOR PARTICLE FOCUSING IN MICROCHANNELS

Номер: US20170080425A1
Автор: Dicarlo Dino, Edd Jon F., Irimia Daniel, Toner Mehmet
Принадлежит:

Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines. 1. A system for focusing and ordering particles suspended within a moving fluid into one or more localized stream lines , comprising:a substrate;at least one expanding spiral-shaped channel having a rectangular cross-section provided on the substrate, wherein the at least one expanding spiral-shaped channel comprises an inlet at or near an inner end of the expanding spiral-shaped channel and at least two outlets at or near an outer end of the expanding spiral-shaped channel, and wherein a radius of curvature of the spiral-shaped channel increases from the inner end to the outer end; anda pumping element configured to move the fluid and particles along the at least one expanding spiral-shaped channel in one direction and in a laminar flow;wherein the fluid, the channel, and the pumping element are configured to cause inertial forces to act on the particles to focus the particles into one or more stream lines and to order the particles with a uniform spacing in the direction of the flow.2196.-. (canceled)197. The system of claim 1 , wherein the expanding spiral-shaped channel comprises three or more outlets.198. The system of claim 1 , wherein the expanding spiral-shaped channel comprises two or more inlets.199. The system of claim 1 , wherein the pumping element is controlled to achieve a flow rate of the fluid of about 0.1 to 5.5 ml/minute.200. The system of claim 1 , ...

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Номер записи: 51
14-03-2019 дата публикации

DISPOSABLE CHIP-TYPE FLOW CELL AND CELL SORTER USING THE SAME

Номер: US20190078995A1
Автор: Takeda Kazuo
Принадлежит: ON-CHIP BIOTECHNOLOGIES CO., LTD.

The object of the present invention is to provide 1a flow cell wherein a flow path is formed in a flat substrate,a illumination unit configured to illuminate particles in a sample liquid flowing through the flow path,a detection unit configured to detect particles of interest by detecting a scattered light or fluorescence generated from the particle when the particle is illuminated, and identifying the particle based on its signal intensity,a pair of oppositely-branched flow paths connected to the flow path for applying a pulse flow to the particles flowing through the flow path in the flow cell from the lateral side,a means for generating the pulse flow from one to the other of the pair of oppositely-branched flow paths, which is connected to the branched flows path via an electromagnetic valve,a means for separating the particle into the branched flow on the side for incorporating the pulse flow, anda control unit configured to control a movement of the electromagnetic valve based on the signal from the detection unit;wherein the flow cell comprises:a flow path for introducing the sample liquid, and a pair of flow paths for introducing a sheath solution arranged on both sides of the flow path to introduce the sample liquid,a joining flow path for joining the flow path to introduce the sample liquid and the pair of flow paths to introduce the sheath liquid and flowing the sheath liquids on either side of the sample liquid,an illumination region on the joining flow path, anda pair of oppositely-branched flow paths connected to the joining flow path, downstream of the illumination region,and wherein the detection unit detects a light signal generated when the particles pass through the illumination region;the control unit judges whether or not the particle is to be separated based on the light signal from the detection unit, and if it is judged that the particle is to be separated, the particle of interest is separated into the branched flow on the side for ...

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Номер записи: 52
12-06-2014 дата публикации

Method and Device for Generating a Tunable Array of Fluid Gradients

Номер: US20140162262A1
Автор: Estes Matthew D., HURTH Cedric M., Zenhausern Frederic
Принадлежит: The Arizona Board of Regents on Behalf of the University of Arizona

Provided herein are devices and methods for generating microfluidic gradients, including an array of unique microfluidic gradients within an array of microchannels. Fluids within conduits are mixed in an intersection region to generate a mixed flow stream in a source reservoir channel that provides a gradient that varies with axial distance from the intersection region. Microchannels having an inlet connected to the source reservoir channel are configured to provide a microfluidic gradient in the microchannel. An outlet end of the microchannel is connected to a sink reservoir channel. By varying the ratio of fluid flow rates from the fluid conduits, the microchannel gradients are tuned. In this manner, a large number of unique gradients or array of microfluidic gradients is provided, wherein the gradient can be any number of physical or chemical parameters, including concentrations and physical fluid properties. 1. A microfluidic gradient generator for tuning dynamic components of fluid comprising:a first fluid conduit;a second fluid conduit,an intersection region that fluidically connects the first fluid conduit and the second fluid conduit, the intersection region comprising an intersection opening between the first fluid conduit and the second fluid conduit and a flow-divider that extends in a downstream direction from the intersection opening;a source reservoir channel fluidically connected to the intersection region and extending downstream from the intersection opening;a sink reservoir channel fluidically connected to the intersection region and extending downstream from the intersection opening;a microchannel array comprising a plurality of microchannels, each microchannel having an inlet end connected to the source reservoir channel and an outlet end connected to the sink reservoir channel, wherein adjacent microchannels are separated from each other by a separation distance, wherein the microchannel array traverses an axial distance along the source ...

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Номер записи: 53
12-03-2020 дата публикации

MICROFLUIDIC CHIP

Номер: US20200080927A1
Автор: Faust Marjorie, Larsen John, PETERSON Shane, Shao Guocheng, Xia Zheng, Zhou Yu
Принадлежит:

A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels. 143-. (canceled)44. An apparatus for identifying components in a fluid mixture , comprising: [ (i) a first intersection; and', '(ii) a second intersection;, '(a) a sample input channel disposed in a first structural layer, said sample input channel for receiving a fluid mixture containing a plurality of components, wherein said sample input channel comprises, '(b) a first plurality of sheath fluid channels disposed in said first structural layer, wherein said first plurality of sheath fluid channels intersect said sample input channel at said first intersection;', '(c) a second plurality of sheath fluid channels disposed in a second structural layer, wherein said second plurality of sheath fluid channels intersect said sample input channel at said second intersection; and', '(d) a plurality of output channels fluidly connected to and branching from said sample input channel, wherein each of said plurality of output channels is disposed between a pair of recessed portions at an end of said first ...

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Номер записи: 54
05-05-2022 дата публикации

FLUIDIC DEVICE FOR THE DETECTION, CAPTURE, OR REMOVAL OF A DISEASE MATERIAL

Номер: US20220134341A1
Автор: BELL Charleson S., COOK Andrew L., GIORGIO Todd D., MILLER Sinead E.
Принадлежит:

The present disclosure relates to a fluidic device to detect, capture, and/or remove disease material in a biological fluid. The present invention also relates to methods for the treatment/prevention of sepsis through the use of the claimed device.

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Номер записи: 55
19-03-2020 дата публикации

FLUIDICS SYSTEM FOR SEQUENTIAL DELIVERY OF REAGENTS

Номер: US20200086317A1
Автор: Marran David, SCHULTZ Jonathan
Принадлежит:

The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion. The invention is particularly advantageous in apparatus for performing sensitive multistep reactions, such as pH-based DNA sequencing reactions. 1. An apparatus comprising:a reagent source;a sensor device defining a plurality of separate flow chambers disposed over a sensor array, each flow chamber having an inlet and an outlet, the inlet in fluid communication with the reagent source and the outlet connected to waste.2. The apparatus of claim 1 , wherein claim 1 , for the each flow chamber claim 1 , the outlet is disposed diagonally opposite the inlet.3. The apparatus of claim 1 , further comprising a wash solution source in fluid communication with the inlet.4. The apparatus of claim 3 , further comprising for each flow chamber a valve disposed between the wash solution source and the inlet.5. The apparatus of claim 3 , further comprising a passage to waste in fluid communication with the reagent source and the wash solution source by passing the flow chamber.6. The apparatus of claim 1 , wherein a single large flow cell defines the plurality of flow chambers over the sensor array.7. The apparatus of claim 1 , further comprising a ...

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Номер записи: 56
19-03-2020 дата публикации

Method and Device for Encapsulating Cell in Liquid Droplet for Single-Cell Analysis

Номер: US20200086321A1
Автор: Je Kwanghwi, Kim Shin-Hyun, MOON Hui-sung, Park Woong-Yang
Принадлежит:

Provided are a method and a device for encapsulating a cell in droplet for single-cell analysis, or a method and a device for forming droplet for single-cell analysis. According to the method and the device of one aspect, by using the effects of inertial ordering, not only a ratio at which one cell is encapsulated in one droplet is increased, but also a yield of generating droplet is improved. 1. A method of forming droplet for single-cell analysis , the method comprising:providing cells;providing one or more distinctly barcoded RNA capture beads; andencapsulating in one droplet a single cell of the cells and a single bead of the one or more distinctly barcoded RNA capture beads,wherein the providing of the cells or the providing of the one or more distinctly barcoded RNA capture beads is provided through inertial ordering before the single cell or the single bead are encapsulated in the one droplet.2. The method of claim 1 , wherein the inertial ordering occurs through a spiral channel of a microfluidic device to which the cells or the one or more distinctly barcoded RNA capture beads are provided.3. The method of claim 1 , wherein the inertial ordering improves a ratio at which the single cell and the single bead are encapsulated in the one droplet.4. The method of claim 1 , wherein the one or more distinctly barcoded RNA capture beads are provided with a cell lysate.5. The method of claim 1 , wherein the one or more distinctly barcoded RNA capture beads include multiple nucleotides or oligonucleotides claim 1 , each molecularly barcoded on a surface of the one or more distinctly barcoded RNA capture beads.6. The method of claim 5 , wherein the multiple nucleotides or oligonucleotides include a nucleotide sequence for capture of mRNAs in the single cell.7. The method of claim 5 , wherein a sequence the multiple nucleotides or oligonucleotides includes an oligo-dT sequence or a primer sequence.8. A microfluidic device for forming droplet for single-cell analysis ...

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Номер записи: 57
19-03-2020 дата публикации

Inertial droplet generation and particle encapsulation

Номер: US20200086322A1
Автор: Arash Jamshidi, Foad Mashayekhi, Hamed AMINI, Tarun Kumar Khurana, Yir-Shyuan WU
Принадлежит: Illumina Inc

Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.

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Номер записи: 58
06-04-2017 дата публикации

Inlet and outlet geometries for a vertical three-stream microfluidic device

Номер: US20170096632A1
Автор: Allison Hubel, Jacob Hanna
Принадлежит: Allison Hubel, Jacob Hanna

An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet.

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Номер записи: 59
14-04-2016 дата публикации

Microfluidics separation method and system thereof

Номер: US20160101419A1
Автор: Cheuk-Wing Li, Guodong Yu, Jingyun JIANG, Ming Yuen LEE
Принадлежит: UNIVERSITY OF MACAU

A microfluidic system for separating an analyte from a sample fluid including a series of fluidic channels including at least one first region and at least one second region. The first region includes a plurality of L-nodes, which connects to each other in series. The second region includes a plurality of R-nodes, which connects to each other in series. The first region is configured to trigger at least about one lamination process cycle for both the sample fluid and the buffer fluid and the second region is configured to trigger at least about one reverse lamination process cycle for both the sample fluid and the buffer fluid, whereby the lamination process cycle and the reverse lamination process cycle causes the analyte to diffuse to the buffer fluid from the sample fluid. A method for separating the analyte is also disclosed.

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Номер записи: 60
12-04-2018 дата публикации

SYSTEM AND METHODS OF CONCENTRATING AIRBORNE PARTICLES

Номер: US20180099283A1
Автор: MAHDAVIPOUR Omid, Paprotny Igor
Принадлежит:

The present disclosure provides for a system and method of concentrating airborne particles, specifically toward the center of one or more intake channels of PM sensors. The invention provides a simple and cost effective device that, when used in conjunction with a MEMS PM sensor or the like, can increase the sensitivity of said device by orders of magnitude. An in-line MEMS PM concentrator uses a converging optical intensity field to concentrate particulate matter along the center of a longitudinal axis of a microchannel. More specifically, the concentrator is designed to bring in ambient air containing PM through a microchannel and concentrate the PM in the center of the microchannel using a converging optical intensity field within a confocal optical cavity. 1. A micro-electro mechanical system (MEMS) particulate matter concentrator comprising:at least one microchannel; andat least one optical cavity disposed within the microchannel wherein the particulate matter is directed toward the center of the microchannel using an in-plane or out-of-plane optical intensity field; andan optical light source providing photons for a converging optical intensity field inside the optical cavity.2. The MEMS particulate matter concentrator of claim 1 , wherein the optical light source is a laser or laser diode.3. The MEMS particulate matter concentrator of claim 1 , wherein the optical light cavity comprises at least two mirrors disposed within the microchannel.4. The MEMS particulate matter concentrator of claim 3 , wherein the two mirrors are concave cylindrical mirrors.5. The MEMS particulate matter concentrator of claim 1 , wherein the optical light cavity comprises a plurality of mirrors disposed along at least a portion of a length of the microchannel.6. The MEMS particulate matter concentrator of claim 1 , wherein the optical light cavity comprises at least as first continuous mirror and a second continuous mirror positioned substantially parallel to one another and ...

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Номер записи: 61
08-04-2021 дата публикации

ISOELECTRIC FOCUSING DEVICES AND FIXTURES

Номер: US20210101152A1
Автор: Arnold Don Wesley, Bousse Luc, Gentalen Erik, Gwerder Eric, MACK Scott
Принадлежит:

Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point. 1. A fixture comprising:an electrode reservoir; anda membrane disposed within the electrode reservoir, wherein the membrane is disposed at a surface of the electrode reservoir,wherein the membrane provides an electrical connection between an electrode positioned within the electrode reservoir and a fluid contained within at least one fluid channel in fluid communication with the electrode reservoir;wherein the electrode reservoir further comprises an insert disposed within the electrode reservoir and positioned at or adjacent to the membrane.2. The fixture of claim 1 , wherein the insert comprises an inlet fluid path arm and an outlet fluid path arm that facilitate substantially bubble-free wetting of the surface of the membrane when the electrode reservoir is filled with a buffer solution.3. The fixture of claim 1 , wherein the membrane comprises a first surface facing the electrode reservoir and a second surface facing the intersection of the inlet fluid channel and the outlet fluid channel claim 1 , wherein a hydrodynamic resistance between the first surface and the second surface is greater than 1 ((N/mm)/(mm/sec)).4. The fixture of claim 3 , wherein an electrical resistance between the first surface and the second surface is less than 10 claim 3 ,000 claim 3 ,000 ohms.5. The fixture of claim 1 , wherein the at least one fluid channel is in fluid communication with a separation channel.6. The fixture of ...

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Номер записи: 62
13-04-2017 дата публикации

Cartridge for a Magnetic Flow Cytometer, a Magnetic Flow Cytometer, and Method for Analysing a Sample With Such a Cartridge

Номер: US20170102313A1
Автор: Hayden Oliver, Helou Michael Johannes, Reisbeck Mathias, Richter Lukas
Принадлежит:

The invention relates to a cartridge () for a magnetic flow cytometer, mainly extending in a x-y-plane, with an inlet () for injecting a sample () into the cartridge (), a blister () for a buffer solution () with magnetic markers to mark pregiven particles (′) of the sample (), an outlet, and a fluid channel (), the fluid channel () comprising a first part that connects the inlet () with the blister () and a second part that connects the first part with the outlet, wherein the second part of the fluid channel () comprises an enrichment zone () with mechanical guiding structures to focus marked particles (′) of the sample () in a predetermined subsection of the fluid channel () and a measuring zone () between the enrichment zone () and the outlet, the measuring zone () comprising a magnetic field sensor () in the predetermined subsection of the fluid channel () in order to provide simplified and accelerated means for measuring particles, in particular concentrations of particles, of a sample. 21. The cartridge () of claim 1 ,characterized by{'b': 4', '4', '9', '15, 'a fluid chamber (), in particular a fluid chamber () in the first part of the fluid channel (), for mixing the sample () with the markers.'}31. The cartridge () of any of the precedent claims claim 1 ,characterized by{'b': 9', '5', '9', '6, 'sub': 1', '2', '3', '4, 'the mechanical guiding structures comprising the fluid channel () decreasing, in particular decreasing stepwise, in its extension (e, e, e, e) in the z-direction in the enrichment zone () as the fluid channel () gets closer to the measuring zone ().'}41. The cartridge () of any of the precedent claims claim 1 ,characterized by{'b': 13', '9', '13', '16', '16, 'the mechanical guiding structures comprising a multitude of elevations (), in particular walls, extending on a surface of the fluid channel () in the x-y-plane, in particular with the elevations () having an extension in the z-direction of more than half of the diameter of the particles ...

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Номер записи: 63
23-04-2015 дата публикации

MICROFLUIDIC DEVICE AND RELATED METHODS

Номер: US20150111239A1
Автор: Collins Scott D., Hock Janet M., Smith Rosemary L.
Принадлежит:

A combinatorial microenvironment generator is configured for the generation of arbitrary, user-defined, steady-state, concentration gradients with negligible to no flow through the growth medium to perturb diffusion gradients or cellular growth. More importantly, the absolute concentrations and/or gradients can be dynamically altered upon request both spatially and temporally to impose tailored concentration fields for in-situ stimulus studies. Here, diffusion occurs via an array of ports, each of which can be an independently controlled source/sink. Together, the array of ports establishes a user-defined, 3D concentration profile. Useful methods related to this device are also provided. 1168-. (canceled)168. A microfluidic device comprisingat least one cell culture chamber configured to receive and retain media;at least three diffusion ports in fluid communication with said cell culture chamber;at least three flow channels functionally connected to said diffusion ports;at least one regulator functionally connected to said flow channels and said diffusion ports to regulate the circulation of at least one material into said flow channels and said diffusion ports to affect a concentration gradient of said at least one material in said cell culture chamber to evaluate the effect of said at least one material on a cell culture in said cell culture chamber,wherein at least one said diffusion port acts as an inlet port for said at least one material and at least one diffusion port acts as an outlet port for said at least one material.169. The microfluidic device of claim 168 , wherein said at least one material is circulated via passive diffusion.170. The microfluidic device of claim 168 , wherein the device further comprises one or more waste channels that are in fluid communication with said cell culture chamber.171. The microfluidic device of claim 168 , wherein the device comprises between 3 and 100 diffusion ports.172. The microfluidic device of claim 168 , wherein ...

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Номер записи: 64
23-04-2015 дата публикации

Inlet and outlet geometries for a vertical three-stream microfluidic device

Номер: US20150111241A1
Автор: Allison Hubel, Jacob Hanna
Принадлежит: University of Minnesota

An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet.

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Номер записи: 65
13-05-2021 дата публикации

Object focusing

Номер: US20210138467A1
Автор: Alexander N. Govyadinov, Pavel Kornilovich, Viktor Shkolnikov
Принадлежит: Hewlett Packard Development Co LP

An object focuser may include a substrate, a sample fluid passage supported by the substrate, a first inertial pump supported by the substrate to pump a sample fluid entraining an object through the sample fluid passage, a first sheath fluid passage, a second inertial pump supported by the substrate to pump a first sheath fluid through the first sheath fluid passage, a second sheath fluid passage and a second inertial pump supported by the substrate to pump a second sheath fluid through the second sheath fluid passage. The first sheath fluid passage and the second sheath fluid passage are connected to the sample fluid passage at a convergence on opposite sides of the sample fluid passage.

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Номер записи: 66
03-05-2018 дата публикации

ELECTRONIC CONTROL OF FLUIDIC SPECIES

Номер: US20180117585A1
Автор: Ahn Keunho, Cheng Zhengdong, Cristobal-Azkarate Galder, Link Darren Roy, Weitz David A.
Принадлежит:

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. In some cases, the droplets may each have a substantially uniform number of entities therein. For example, 95% or more of the droplets may each contain the same number of entities of a particular species. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction. In a related aspect of the invention, systems and methods for allowing fluid mixing within droplets to occur are also provided. In still another aspect, the invention relates to systems and methods for sorting droplets, e.g., by causing droplets to move to certain regions within a fluidic system. Examples include using electrical interactions (e.g., charges, dipoles, etc.) or mechanical systems (e.g., fluid displacement) to sort the droplets. In some cases, the fluidic droplets can be sorted at relatively high rates, e.g., at about 10 droplets per second or more. Another aspect of the invention provides the ability to determine droplets, or a component thereof, for example, using fluorescence and/or other optical techniques (e.g., microscopy), or electric sensing techniques such as dielectric sensing. 1273-. (canceled)274. A method of sorting droplets in a microfluidic system , the method comprising:providing a series of droplets of fluid surrounded by a liquid and flowing in a ...

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Номер записи: 67
03-05-2018 дата публикации

SYSTEMS AND METHODS FOR PARTICLE FOCUSING IN MICROCHANNELS

Номер: US20180117593A1
Автор: Dicarlo Dino, Edd Jon F., Irimia Daniel, Toner Mehmet
Принадлежит:

Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines. 1. A system for focusing particles suspended within a moving fluid into one or more localized stream lines , comprising:a substrate;at least one channel provided on the substrate, the at least one channel having an inlet and an outlet;a fluid moving along the at least one channel in a laminar flow and including suspended particles;a pumping element driving the laminar flow of the fluid;wherein the fluid, the channel, and the pumping element are configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.2196.-. (canceled) The present application claims priority to U.S. Provisional Application No. 60/923,609 filed on Apr. 16, 2007 and entitled “Methods and Devices for Separating and Focusing Particles,” U.S. Provisional Application No. 60/923,837 filed on Apr. 17, 2007 and entitled “Methods and Devices for Separating and Focusing Particles,” and U.S. Provisional Application No. 60/999,131 filed on Oct. 16, 2007 and entitled “Methods and Devices for Separating and Focusing Particles,” all three of which are expressly incorporated herein by reference in their entireties.Particle separation and filtration has been applied for numerous technological solutions in industry, medicine, and research. Industrial applications include chemical process and fermentation filtration, water purification for the microelectronics ...

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Номер записи: 68
25-04-2019 дата публикации

MICROFLUIDIC DEVICE FOR FULL BLOOD COUNT

Номер: US20190120840A1
Автор: GWYER JAMES D., PETTIGREW DAVID M.
Принадлежит:

A microfluidic device () for full blood count includes a first measurement channel () and a second measurement channel () separated from the first measurement channel (). The microfluidic device () furthermore includes a first inlet () for providing a whole blood sample to the first and second measurement channel (), a second inlet () for providing a lysis agent for white blood cell count in to the first channel (), a third inlet () for providing a quench solution to the first channel (), and a fourth inlet () for providing a lysis agent for hemoglobin measurement to the second channel (). A method for forming such a microfluidic device () and a method for performing a full blood count test using such a microfluidic device () are described. 1. A method for manufacturing a microfluidic device for full blood count , the method comprising:providing a first measurement channel,providing a second measurement channel the second measurement channel separated from the first measurement channel,providing a first inlet for providing a whole blood sample to the first and second measurement channel,providing a second inlet at the first measurement channel for providing a lysis agent for white blood cell count in to the first channel,providing a third inlet at the first measurement channel for providing a quench solution to the first channel, andproviding a fourth inlet at the second measurement channel for providing a lysis agent for hemoglobin measurement to the second channel.2. A method for performing full blood count , the method comprising:providing a blood sample to a first and a second measurement channel of a microfluidic device, the first and second measurement channel being separated from each other,providing a lysis agent suitable for white blood cells to the blood sample in the first channel,providing a quench solution to the blood sample in the first channel,providing a lysis agent for hemoglobin to the blood sample in the second channel,at the end of the first ...

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Номер записи: 69
16-04-2020 дата публикации

PARTICLE SENSOR AND PARTICLE SENSING METHOD

Номер: US20200116604A1
Автор: Chen Shuang, Kelly Declan Patrick, Ronda Cornelis Reinder, SCHEJA MICHAEL MARTIN
Принадлежит:

A particle sensing system is for sensing particles entrained in a fluid. The system comprises a flow channel having a longitudinal direction along which the fluid is to be passed, a heating arrangement for heating the fluid and thereby applying a positive thermophoretic force on the fluid in a direction perpendicular to the longitudinal direction of the flow channel and a first sensor for sensing the particles in the fluid after heating by the heating arrangement. The thermophoretic force increases the concentration of the particles at the first sensor. 1. A particle sensing system for sensing particles entrained in a fluid , comprising:a detection volume located within a flow channel through which the fluid is to be passed in a longitudinal direction;a heating arrangement for generating a thermal gradient perpendicular to the fluid flow direction and thereby applying a positive thermophoretic force to particles entrained in the fluid; anda sensor for sensing the particles in the fluid at the detection volume, wherein the thermophoretic force is used to constrict the space occupied by the particles within the flow and thereby concentrate the particles within the detection volume,wherein the heating arrangement is adjustable to provide different levels of thermophoretic force on the fluid, andwherein the system further comprises a controller which is adapted to:obtain an initial particle concentration measurement with no heating; andcontrol the heating arrangement in dependence on the initial particle concentration measurement.2. A system as claimed in claim 1 , wherein the heating arrangement comprises a plurality of independently controllable heating elements.3. A system as claimed in claim 1 , wherein the detection volume has a smaller cross sectional area than that of the flow channel.4. A system as claimed in claim 1 , wherein the heating arrangement comprises a coil or set of coils surrounding the flow channel.5. A system as claimed in claim 1 , wherein the ...

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Номер записи: 70
27-05-2021 дата публикации

METHODS AND DEVICES FOR MULTI-STEP CELL PURIFICATION AND CONCENTRATION

Номер: US20210156787A1
Автор: "DSILVA Joseph", AURICH Lee, Austin Robert H., CIVIN Curt, GANDHI Khushroo, GRISHAM Michael, SKELLEY Alison, STURM James C., WARD Anthony
Принадлежит:

Described herein are microfluidic devices and methods that can separate and concentrate particles in a sample. 1. A method for separating white blood cells or stem cells from platelets in a sample , comprising passing the sample through a system , wherein , prior to being exposed to the magnetic separator , white blood cells or stem cells are labelled magnetically and wherein the system comprises:a) a microfluidic channel comprising a first array of obstacles wherein said obstacles are configured to separate cells by deterministic lateral displacement (DLD) based on their sizes, such that white blood cells or stem cells flow in a first direction and platelets flow in a second direction different from the first direction;b) a magnetic separator comprising configured to separate particles or cells with magnetically susceptible labels from particles or cells without magnetically susceptible labels, wherein the first array of obstacles is fluidically connected with the magnetic separator;c) a particle sensor which is fluidically connected to the DLD array or magnetic separator and which, in response to particles or cells with magnetically susceptible labels arriving in a sensing zone, generates an actuation signal to create an actuation event; ii) the particle sensor of paragraph c); and', 'iii) a switch configured to receive the actuation signal., 'd) a particle dispenser fluidically connected to the DLD array or magnetic separator for dispensing particles to a particle collector, wherein the particle dispenser comprises2. The method of claim 1 , wherein the obstacles in the microfluidic channel have a polygonal cross-section.3. The method of claim 2 , wherein: a vertex of each of two adjacent obstacles points toward each other in a direction substantially perpendicular to a flow direction of the sample through the array of obstacles.4. The method of claim 2 , wherein the DLD array comprises an array of obstacles that are diamond-shaped claim 2 , tear drop shaped or ...

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Номер записи: 71
10-05-2018 дата публикации

METHOD AND DEVICE FOR HIGH THROUGHPUT CELL DEFORMABILITY MEASUREMENTS

Номер: US20180128735A1
Автор: Di Carlo Dino, Gossett Daniel R., Tse Henry T.K.
Принадлежит: The Regents of the University of California

A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures. 131-. (canceled)32. A system for measuring a change in a morphological parameter of a cell comprising:a substrate containing an inlet microfluidic channel and one or more outlet microfluidic channels;an extensional region comprising an intersection of the inlet microfluidic channel and the one or more outlet microfluidic channels, wherein the inlet microfluidic channel and the one or more outlet microfluidic channels intersect in a T-shaped intersection;a focusing region located in the inlet microfluidic channel and upstream of the extensional region, wherein the focusing region focuses cells passing there through into a stream of cells located along one or more lateral streamlines;an optical collector configured to capture multiple records of diffracted or refracted light from each cell passing through the extensional region;a pump configured to flow a plurality of cells into the inlet microfluidic channel and through the extensional region, wherein the plurality of cells flow ...

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Номер записи: 72
02-05-2019 дата публикации

Microfluidic system and method for arranging objects

Номер: US20190126277A1
Автор: Lucas Frenz, Lung-Hsin HUNG, Robert MELTZER
Принадлежит: Bio Rad Laboratories Inc

Microfluidic systems and methods for arranging a set of objects. In an exemplary method, the set of objects may be transported in carrier fluid along a microfluidic channel structure having a reformatting zone including an object-accessible region and at least one object-excluding region. A portion of the carrier fluid may be moved from the object-accessible region to the at least one object-excluding region in an upstream section of the reformatting zone, to reduce a spacing of objects of the set. The portion of the carrier fluid may be directed into the object-accessible region from the at least one object-excluding region in a downstream section of the reformatting zone, to increase a spacing of objects of the set. The steps of moving and directing in combination may increase the spacing between objects disproportionately for a subset of the objects that are closest to one another.

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Номер записи: 73
23-04-2020 дата публикации

METHOD AND DEVICE FOR HIGH-THROUGHPUT SOLUTION EXCHANGE FOR CELL AND PARTICLE SUSPENSIONS

Номер: US20200122056A1
Автор: Di Carlo Dino, Gossett Daniel R., Tse Henry T.K.
Принадлежит: The Regents of the University of California

A method of exchanging fluids with suspended particles includes providing a microfluidic device with a first inlet channel operatively coupled to a source of particles and a second inlet channel operatively coupled to an exchange fluid. A transfer channel is connected at a proximal end to the first inlet channel and the second inlet channel. First and second outlet channels are connected to a distal end of the transfer channel. The source of particles is flowed at a first flow rate into the first inlet channel while the exchange fluid is flowed at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5. Particles are collected in one of the first and second outlet channels while fluid substantially free of particles is collected in the other of the first and second outlet channels. 133-. (canceled)34. A method of transferring particles across fluid streamlines comprising:providing a microfluidic device comprising a plurality of proximal channels and a plurality of distal channels connected to a transfer channel disposed between the plurality of proximal channels and the plurality of distal channels, wherein at least one of the proximal channels is operatively coupled to a source of fluid containing particles suspended therein, at least one of the proximal channels is operatively coupled to a source of exchange fluid;flowing the fluid containing the particles in the at least one proximal channel;{'sub': 'eq', 'flowing the exchange fluid into the at least one proximal channel operatively coupled to the source of exchange fluid, wherein a co-flow state of the fluid containing the particles suspended therein and the exchange fluid is maintained along substantially all of the transfer channel, wherein at least some of the particles of the fluid containing the particles suspended therein migrate in response to inertial lift forces toward an equilibrium position (X) located within the transfer ...

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Номер записи: 74
23-04-2020 дата публикации

MULTILAYER HYDRODYNAMIC SHEATH FLOW STRUCTURE

Номер: US20200122937A1
Автор: Bunner Bernard, Deshpande Manish, Gilbert John R.
Принадлежит:

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip. 1. A microfabricated sheath flow structure for suspending a stream of particles in a sheath fluid , comprising:a primary sheath flow channel for conveying a sheath fluid, wherein the primary sheath flow channel has a width and a height;a sample inlet intersecting the primary sheath flow channel at a sample injection site for injecting a stream of particles into the sheath fluid conveyed through the primary sheath flow channel;a primary focusing region downstream of the sample injection site that focuses the stream of particles in at least a vertical direction; anda secondary focusing region downstream of the primary focusing region that focuses the stream particles in at least a horizontal direction.2. The microfabricated sheath flow structure of claim 1 , wherein the primary focusing region further focuses in a horizontal direction.3. The microfabricated sheath flow structure of claim 1 , wherein the secondary focusing region further focuses in a vertical direction.4. The microfabricated sheath flow structure of claim 1 , wherein a height of the primary sheath flow channel is reduced in the primary focusing region to produce the vertical focusing.5. The microfabricated sheath flow structure of claim 3 , wherein a height of the primary sheath ...

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Номер записи: 75
23-04-2020 дата публикации

METHOD OF OPTIMIZING MICROPARTICLE SUCTION CONDITION AND MICROPARTICLE SEPARATION DEVICE

Номер: US20200124517A1
Автор: Hashimoto Gakuji, ITO Tatsumi, Kajihara Junji, TAKAHASHI Kazuya
Принадлежит:

In the present technology, the timing at which suction is performed is optimized in order to enhance the microparticle separation performance in a technology for separating target microparticles in a microchip. For this purpose, the present technology provides a method of optimizing a microparticle suction condition, and the like, using a microchip having a main flow channel through which a liquid containing a microparticle flows, a microparticle suction flow channel arranged coaxially with the main flow channel, and a branch flow channel branching from the main flow channel. The method includes: a branch point specifying process of specifying a branch point at which the branch flow channel branches from the main flow channel; and a time assignment process of assigning a time Tto be applied to suction of the microparticle, on the basis of a distance between a position of the microparticle and the branch point at a time point when a predetermined time Thas elapsed from a time when the microparticle passed through a predetermined position on the main flow channel. 1. A method of optimizing a microparticle suction condition ,using a microchip having a main flow channel through which a liquid containing a microparticle flows, a microparticle suction flow channel arranged coaxially with the main flow channel, and a branch flow channel branching from the main flow channel, the method comprising:a branch point specifying process of specifying a branch point at which the branch flow channel branches from the main flow channel; and{'sub': 1', 'X, 'a time assignment process of assigning a time Tto be applied to suction of the microparticle, on a basis of a distance between a position of the microparticle and the branch point at a time point when a predetermined time Thas elapsed from a time when the microparticle passed through a predetermined position on the main flow channel.'}2. The method of optimizing the microparticle suction condition according to claim 1 , further ...

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Номер записи: 76
17-05-2018 дата публикации

SYSTEMS FOR HANDLING MICROFLUIDIC DROPLETS

Номер: US20180135117A1
Автор: Link Darren Roy
Принадлежит:

The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel 1. A method for analyzing a nucleic acid , the method comprising:flowing an aqueous fluid comprising nucleic acids and loci-specific primers through a microfluidic channel into a counter-propagating stream of oil to form a plurality of aqueous droplets, wherein each of the plurality of aqueous droplets comprises a single nucleic acid and a plurality of the loci-specific primers;conducting an amplification reaction in each of the plurality of aqueous droplets to produce amplicons corresponding to two or more loci of the single nucleic acid in each of the plurality of aqueous droplets;releasing the amplicons from the plurality of aqueous droplets; andanalyzing the ...

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Номер записи: 77
08-09-2022 дата публикации

Apparatus And System For Biofluid Sample Dispensing And/Or Assay

Номер: US20220280944A1
Автор: Cheng Yuen Hsia, Liu Feng, Lynn Charm Nyein, Navawongse Rapeechai, Pulido Roland Galang, Thaveeprungsriporn Visit, Wang Ying Song
Принадлежит: NITTO DENKO CORPORATION

An apparatus for assaying biofluid includes a receptacle for removably housing a set of cuvettes of a biofluid sample dispensing apparatus, each cuvette of the set of cuvettes containing an assay sample having a reagent and a sample of the biofluid; a receptacle sealing element disposed around the receptacle for providing sealing engagement between the receptacle and the biofluid sample dispensing apparatus for sealing the set of cuvettes within the receptacle; a vacuum source for evacuating the receptacle; and an automated assay system connected to the receptacle for performing an assay process on the assay samples in the set of cuvettes, while maintaining the set of cuvettes within the receptacle. 1. An apparatus for assaying biofluid , the apparatus comprising:a receptacle for removably housing a set of cuvettes of a biofluid sample dispensing apparatus, each cuvette of the set of cuvettes containing an assay sample comprising a reagent and a sample of the biofluid;a receptacle sealing element disposed around the receptacle for providing sealing engagement between the receptacle and the biofluid sample dispensing apparatus for sealing the set of cuvettes within the receptacle;a vacuum source for evacuating the receptacle; andan automated assay system connected to the receptacle for performing an assay process on the assay samples in the set of cuvettes, while maintaining the set of cuvettes within the receptacle.2. The apparatus according to claim 1 , wherein the receptacle comprises a set of receptacle sockets claim 1 , each receptacle socket of the set of receptacle sockets for receiving a respective cuvette of the set of cuvettes.3. The apparatus according to claim 2 , wherein the set of receptacle sockets are arranged such that the biofluid sample dispensing apparatus is receivable by the receptacle in only one orientation.4. The apparatus according to claim 2 , wherein the set of receptacle sockets are arranged such that the assay process is performable on ...

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Номер записи: 78
09-05-2019 дата публикации

Microfluidic systems and methods of use

Номер: US20190134633A1
Автор: Kevin Ness, Rajiv Bharadwaj, Tobias Daniel Wheeler
Принадлежит: 10X Genomics Inc

Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

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Номер записи: 79
30-04-2020 дата публикации

DEVICES AND METHODS FOR SEPARATING PARTICLES

Номер: US20200129981A1
Автор: Mao Leidong, Zhao Wujun
Принадлежит:

Methods and devices for non-invasive, label-free separation of circulating tumors cells in blood are provided. Embodiments of the disclosure provide for devices employing magnetic fluids and magnets for separation of viable circulating tumor cells from blood. Also described are systems for separation and collection of components of fluid including blood. 1. A method for separating circulating tumor cells from blood cells in a sample of whole blood , comprising:lysing red blood cells from the sample to form a first fluid comprising a cell mixture;introducing the first fluid to a device having a microfluidic channel having a first end and a second end, where the first fluid is introduced into the microfluidic channel through a first inlet, and flowing the first fluid through the microfluidic channel;introducing a second fluid comprising a magnetic fluid into the microfluidic channel through a second inlet located after the first inlet to combine the second fluid with the first fluid to form a third fluid, and hydrodynamically focusing the third fluid into a sheath flow, wherein the third fluid includes components of the first fluid and the second fluid;exposing the third fluid to a magnetic field produced by one or more magnets positioned adjacent and along a length of an area of the microfluidic channel after the second inlet, wherein the magnets have a flux density of about 0 T to about 10 T and a magnetic gradient of about 0 to about 1000 T/m, and wherein the magnetic field produces a magnetization direction substantially perpendicular to the flow of the third fluid in the microfluidic channel;separating the components of the third fluid as a function of component size and width of the microfluidic channel; andcollecting portions of the components of the third fluid in two or more outlet channels positioned after the one or more permanent magnets at the second end of the microfluidic channel.2. The method of claim 1 , wherein the magnetic fluid is a ferrofluid ...

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Номер записи: 80
10-06-2021 дата публикации

MICROPARTICLE SORTING MICROCHIP AND MICROPARTICLE SORTING APPARATUS

Номер: US20210170402A1
Автор: ITO Tatsumi, Matsumoto Masahiro, TAKAHASHI Kazuya
Принадлежит: SONY CORPORATION

A microparticle sorting microchip for a flow cytometer is provided to enable sorting of microparticles at higher speed, higher purity, and higher acquisition rate. The microparticle sorting microchip includes a main channel through which a microparticle-containing fluid flows, a trap channel coaxially communicating with the main channel, a trap chamber communicating with the trap channel, and a gate channel intersecting the trap channel. The trap channel has an opening intersecting the gate channel. The trap channel has a smaller cross-sectional area upstream of the opening than downstream of the opening along a direction in which the microparticle-containing fluid flows. 1. A microparticle sorting microchip comprising:a main channel through which a microparticle-containing fluid flows;a trap channel coaxially communicating with the main channel;a trap chamber communicating with the trap channel; anda gate channel intersecting the trap channel, whereinthe trap channel has an opening portion intersecting the gate channel, andan upstream cross-sectional area of the opening portion is smaller than a downstream cross-sectional area of the opening portion, along a direction in which the microparticle-containing fluid flows.2. The microparticle sorting microchip of claim 1 , whereinthe gate channel includes plural gate channels, and the plural gate channels are connected to an upstream end of the downstream-stage trap channel so that the plural gate channels are symmetrical to each other with respect to a center of a flow of the microparticle-containing fluid from an opening at a downstream end of the upstream-stage trap channel.3. The microparticle sorting microchip of claim 1 , whereinthe gate channel is configured to allow a gate flow fluid to always flow at a constant flow rate.4. The microparticle sorting microchip of claim 3 , whereinin the downstream-stage trap channel, the gate flow fluid is branched to flow from the gate channel in a direction toward the upstream ...

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Номер записи: 81
15-09-2022 дата публикации

ANALYTE DEPLETION FOR SENSOR EQUILIBRATION

Номер: US20220288582A1
Автор: Harmon Ian
Принадлежит:

Methods include treating a portion of a sample composition to be tested for presence of an analyte by depleting or blocking the target analyte. The treated composition may be used to equilibrate an acoustic wave sensor prior to exposing the sensor to the untreated sample composition for analysis. By using the treated sample composition, in which the analyte is depleted or blocked, to equilibrate the sensor, the sensor may be equilibrated with a composition having a similar viscosity and non-specific binding characteristics to the untreated sample composition, which should result in improved accuracy when analyzing the analyte in the untreated sample composition. 116-. (canceled)17. A method comprising:providing a composition to be tested for the presence of an analyte;contacting a first portion of the composition with a material configured to remove or block the analyte from the composition;flowing the first portion of the composition over a surface of an acoustic wave sensor, wherein an analyte capture ligand is bound to the surface of the acoustic wave sensor; andflowing a second portion of the composition over the surface of the acoustic wave sensor, wherein the second portion of the composition has not been contacted with the material configured to remove or block the analyte.18. The method of claim 17 , further comprising driving the acoustic wave sensor into oscillating motion and monitoring an output wave propagation characteristic from the acoustic wave sensor while the first portion of the composition is flowing over the surface of the acoustic wave sensor.19. The method of claim 18 , further comprising:flowing the second portion of the composition over the surface of the acoustic wave sensor after the first portion of the composition has been flowed over the surface of the acoustic wave sensor;monitoring the wave propagation characteristic of the acoustic wave sensor while the second portion of the composition is flowing over the surface of the acoustic ...

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Номер записи: 82
21-08-2014 дата публикации

Devices and methods for programming fluid flow using sequenced microstructures

Номер: US20140230909A1
Автор: Dino Di Carlo, Elodie Sollier, Hamed AMINI
Принадлежит: UNIVERSITY OF CALIFORNIA

A microfluidic platform is disclosed that uses obstacles placed at particular location(s) within the channel cross-section to turn and stretch fluid. The asymmetric flow behavior upstream and downstream of the obstacle(s) due to fluid inertia manifests itself as a total deformation of the topology of streamlines that effectively creates a tunable net secondary flow. The system and methods passively creates strong secondary flows at moderate to high flow rates in microchannels. These flows can be accurately controlled by the numbers and particular geometric placement of the obstacle(s) within the channel.

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Номер записи: 83
07-05-2020 дата публикации

SYSTEMS AND METHODS FOR PARTICLE FOCUSING IN MICROCHANNELS

Номер: US20200139372A1
Автор: Dicarlo Dino, Edd Jon F., Irimia Daniel, Toner Mehmet
Принадлежит:

Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines. 1196-. (canceled)198. The method of claim 197 , wherein the tags comprise beads.199. The method of claim 197 , wherein the tags comprise antibodies.200. The method of claim 197 , wherein the tags comprise magnetic beads.201. The method of claim 197 , wherein the tags comprise quantum dots.202. The method of claim 197 , further comprising focusing the tagged particles into a particular stream line based on the changed size.203. The method of claim 197 , wherein the system further comprises a channel branch and wherein the method further comprises focusing the tagged particles into the channel branch based on the changed size.204. The method of claim 197 , wherein the one or more localized stream lines each define a width that is substantially equal to or greater than the diameter of the tagged particles claim 197 , and the tagged particles suspended in the fluid are focused into the one or more localized stream lines claim 197 , and wherein the number and relative cross-sectional position of the one or more localized stream lines are uniquely defined by the aspect ratio and the particle Reynolds number.205. The method of claim 197 , wherein the tagged particles suspended in the fluid are focused into each of at least two localized stream lines.206. The method of claim 197 , further comprising a curved channel connected to the outlet of the at least one channel claim 197 , ...

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Номер записи: 84
07-05-2020 дата публикации

MICROPARTICLE SORTING METHOD AND MICROCHIP FOR SORTING MICROPARTICLES

Номер: US20200139408A1
Автор: ITO Tatsumi
Принадлежит:

There is provided a microparticle sorting method including a procedure of collecting a microparticle in a fluid that flows through a main channel in a branch channel that is in communication with the main channel by generating a negative pressure in the branch channel. In the procedure, a flow of a fluid is formed that flows toward a side of the main channel from a side of the branch channel at a communication opening between the main channel and the branch channel. 1. A microchip , comprising:a first fluid channel through which a first fluid flows, wherein the first fluid comprises of a plurality of microparticles;a branch channel in communication with the first fluid channel via a first communication opening;a switching portion communicated with an actuator to generate a pressure to deflect a selected microparticle of the plurality of microparticles into the branch channel; and a second fluid flows from the second fluid channel to the second communication opening, and', 'the second fluid channel is perpendicular to the branch channel., 'a second fluid channel in communication with the branch channel via a second communication opening in the branch channel, wherein'}2. The microchip according to claim 1 , wherein the switching portion is further configured to:generate a force based on a change in a voltage applied to the actuator; andchange the pressure in the branch channel based on the force.3. The microchip according to claim 2 , whereinthe actuator is configured to apply a displacement on a surface of the microchip based on the applied voltage, andthe actuator is at a position, corresponding to the branch channel, on the surface of the microchip.4. The microchip according to claim 1 , whereinthe switching portion comprising a pressure chamber configured to communicate with the branch channel, andthe pressure chamber is configured to produce a change in volume of the branch channel.5. The microchip according to claim 1 , whereinthe first fluid flows in a first ...

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Номер записи: 85
22-09-2022 дата публикации

APPARATUS AND METHOD FOR SORTING MICROFLUIDIC PARTICLES

Номер: US20220297124A1
Автор: BASHTANOV Mikhail, Gold Richard, HAYES Calum, HUSSAIN Fred, PRITCHARD Robyn, ROGERS Salman Samson, VARELAS Nuno, ZHUKOV Alexander
Принадлежит:

A single junction sorter for a microfluidic particle sorter, the single-junction sorter comprising: an input channel, configured to receive a fluid containing particles; an output sort channel and an output waste channel, each connected to the input channel for receiving the fluid therefrom; a bubble generator, operable to selectively displace the fluid around a particle to be sorted and thereby to create a transient flow of the fluid in the input channel; and a vortex element, configured to cause a vortex in the transient flow in order to direct the particle to be sorted into the output sort channel. 1. A method for sorting particles in a particle sorter , the method comprising:receiving, at an input channel, an input particle suspension comprising a fluid and a plurality of particles;aligning the plurality of particles in a streamline of the fluid;evaluating the plurality of particles at a predetermined location and assigning a sort designation or a reject designation for each particle of the plurality of particles;responsive to a sort designation, causing a creation of a transient flow in the fluid,wherein the transient flow is created at a position downstream of the predetermined location;responsive to the transient flow, causing formation of a sorting vortex in the fluid; anddirecting particles assigned with the sort designation to an output sort channel via the sorting vortex.2. The method of claim 1 , wherein the input particle suspension is an aqueous suspension of lymphocytes having a diameter of approximately 8 μm and a density of up to approximately 4×10cells/ml.3. The method of claim 2 , wherein the plurality of particles in the streamline is aligned via at least one of inertial focusing claim 2 , hydrodynamic focusing claim 2 , acoustic focusing claim 2 , or dielectrophoretic focusing.4. The method of claim 3 , wherein the streamline comprises a flow velocity of approximately 1 m/s to approximately 4 m/s.5. The method of claim 4 , wherein the flow ...

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Номер записи: 86
24-06-2021 дата публикации

SYSTEMS AND METHODS FOR PARTICULATE ENCAPSULATION IN MICRODROPLETS

Номер: US20210187508A1
Автор: Brenan Colin J.H., Brenan Michael J., Reichen Marcel, SCHERR Steven
Принадлежит:

The present invention generally relates to microfluidic droplets and, in particular, to multiple emulsion microfluidic droplets. Provided are methods and a device of ordering, sorting and/or focusing particles, the method comprising leading the particles through a microfluidic channel comprising a channel height (H) in the range of (1.8) D to (1.2) D and a channel width (W) in the range of (1.33) D to 1 D, wherein D is the particle diameter. 1. A method of ordering , sorting and/or focusing particles , the method comprising leading the particles through a microfluidic channel comprising a channel height (H) in the range of 1.8 D to 1.2 D , wherein D is the particle diameter.2. The method of claim 1 , wherein the microfluidic channel comprises a channel width (W) in the range of 1.33 D to 1 D.3. The method of claim 1 , wherein the microfluidic channel comprises a chamber for a particle reservoir claim 1 , wherein the chamber height requires 1.2 to 1.8 times the particle diameter and the chamber width is at least greater than twice the particle diameter.4. The method of claim 1 , wherein the particles are packed in the chamber before entering the microfluidic channel.5. The method of claim 3 , wherein the chamber comprises tapered lines leading to the microchannel.6. The method of claim 1 , wherein the microfluidic channel height is decreased at the exit.7. The method of claim 1 , wherein the inner wall of the microfluidic channel is hydrophobic.8. The method of claim 1 , wherein the particles are composed of a polymer material with an elastic modulus.9. The method of claim 1 , wherein the particles are hydrogel beads.10. The method of claim 1 , wherein the particles comprise capture molecules.11. The method of wherein the capture molecules may be selected from the group comprising claim 10 , an antigen claim 10 , an antibody or fragments thereof claim 10 , nucleic acids claim 10 , magnetic particles claim 10 , colloidal particles claim 10 , nanoparticles claim 10 , ...

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Номер записи: 87
23-05-2019 дата публикации

METHOD FOR SORTING CELL PARTICLE IN SOLUTION

Номер: US20190154563A1
Автор: Takeda Kazuo
Принадлежит: ON-CHIP BIOTECHNOLOGIES CO., LTD.

A method for sorting a particle of interest in a solution includes storing a sample solution in a sample solution reservoir and applying a constant pressure to the sample solution; illuminating the sample solution flowing in an illumination region of the sample solution flow path upstream of a branched flow path region, and detecting the particle of interest contained in the sample solution; and upon detecting the particle of interest, opening an electromagnetic valve or electromagnetic valves that are disposed between an air pressure pump and the branched flow path region of the sample solution flow path at a timing that the detected particle of interest reaches the branched flow path region such that a pulse pressure is applied to the branched flow path region to separate the detected particle of interest from the sample solution flow path. 1. A method for sorting a particle of interest in a solution , comprising:storing a sample solution in a sample solution reservoir and applying a constant pressure to the sample solution such that the sample solution stored in the sample solution reservoir flows through a sample solution flow path at a predetermined velocity;illuminating the sample solution flowing in an illumination region of the sample solution flow path upstream of a branched flow path region where a pair of branched flow paths are connected to the sample solution flow path, and detecting the particle of interest contained in the sample solution by identifying a scattered light or fluorescence generated from the particle of interest; andupon detecting the particle of interest, opening an electromagnetic valve or electromagnetic valves that are disposed between an air pressure pump and the branched flow path region of the sample solution flow path at a timing that the detected particle of interest reaches the branched flow path region such that a pulse pressure is applied to the branched flow path region to separate the detected particle of interest from the ...

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Номер записи: 88
14-06-2018 дата публикации

Sorting particles in a microfluidic device

Номер: US20180161775A1
Автор: Kyle C. Smith, Mehmet Toner, Ravi Kapur
Принадлежит: General Hospital Corp

A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

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Номер записи: 89
30-05-2019 дата публикации

SYSTEMS AND METHODS FOR PARTICLE FOCUSING IN MICROCHANNELS

Номер: US20190160465A1
Автор: Dicarlo Dino, Edd Jon F., Irimia Daniel, Toner Mehmet
Принадлежит:

Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines. 1196-. (canceled)198. The system of claim 197 , wherein the one or more localized stream lines each define a width that is substantially equal to or greater than the diameter of the particles claim 197 , and the particles suspended in the fluid are focused into the one or more localized stream lines claim 197 , and wherein the number and relative cross-sectional position of the one or more localized stream lines are uniquely defined by the aspect ratio and the particle Reynolds number.199. The system of claim 197 , wherein the particles suspended in the fluid are focused into each of at least two localized stream lines.200. The system of claim 197 , further comprising a curved channel connected to the outlet of the at least one channel claim 197 , wherein when the fluid is flowed through the at least one channel claim 197 , the particles are focused into a single localized stream line formed in the curved channel.201. The system of claim 200 , wherein the curved channel is sigmoidal.202. The system of claim 197 , wherein a ratio of the particle diameter to the at least one channel hydraulic diameter is greater than or equal to about 0.07 and less than or equal to about 0.5.203. The system of claim 197 , wherein the at least one channel is dimensioned and configured to focus particles having a particle diameter in a range of 0.01 micrometers to 40 micrometers.204. The ...

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Номер записи: 90
30-05-2019 дата публикации

ANALYSIS CELL, ANALYSIS DEVICE, ANALYSIS APPARATUS, AND ANALYSIS SYSTEM

Номер: US20190160466A1
Автор: Aoki Hiroyoshi, MATSUYAMA Norihiro, MITA Kanji, Tanaka Yuki, Usui Kengo, Yamagata Yutaka
Принадлежит:

The present invention provides a tool that can analyze a target in a sample with simple operations and can be downsized, and an analysis method using the same. The analysis cell of the present invention includes: a main substrate: a sample inlet cover member; and a gas outlet cover member. The main substrate includes a flow path, an inlet for a sample, and a gas outlet, and the inlet and the gas outlet communicate with an outside. The inlet communicates with an upstream end portion of the flow path and the gas outlet communicates with a downstream end portion of the flow path. The flow path has a shape that expands from an upstream side toward a downstream side of the flow path. The sample inlet cover member is a liquid-tight member and can be fixed to the inlet when the sample inlet cover member is in use. The gas outlet cover member is a liquid-tight and gas-permeable member and can be fixed to the gas outlet when the gas outlet cover member is in use. 1. An analysis cell comprising:a main substrate;a sample inlet cover member; anda gas outlet cover member,wherein the main substrate comprises a flow path, an inlet for a sample, and a gas outlet, and the inlet and the gas outlet communicate with an outside,the inlet communicates with an upstream end portion of the flow path and the gas outlet communicates with a downstream end portion of the flow path,the flow path has a shape that expands from an upstream side toward a downstream side of the flow path,the sample inlet cover member is a liquid-tight member and can be fixed to the inlet when the sample inlet cover member is in use, andthe gas outlet cover member is a liquid-tight and gas-permeable member and can be fixed to the gas outlet when the gas outlet cover member is in use.2. The analysis cell according to claim 1 , whereinthe main substrate has at least two gas outlets, andthe two gas outlets are disposed in a direction perpendicular to a flow direction of the flow path.3. The analysis cell according to ...

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Номер записи: 91
21-05-2020 дата публикации

A SAMPLE CARTRIDGE FOR INCUBATING AND/OR ANALYZING A DISPERSION OF PARTICLES, CELLS OR DROPLETS

Номер: US20200156077A1
Автор: Ermantraut Eugen, Schulz Torsten, STEINMETZER KATRIN, WOLF ALRIK
Принадлежит:

The present invention relates to a sample cartridge for incubating and/or analyzing a dispersion of particles, cells or droplets and/or for performing biochemical reactions with or in such dispersion. The present invention furthermore relates to a device for incubating a dispersion of particles, cells or droplets and/or for performing a biochemical reaction therewith. Moreover, the present invention also relates to the use of a sample cartridge or of a device for generating and/or processing a dispersion of particles, cells or droplets. Moreover, the present invention relates to a method of processing a dispersion of particles, cells or droplets. Furthermore, the present invention relates to a method of generating a dispersion of droplets and to a method of generating a dispersion of solid or semi-solid particles. 1. A sample cartridge for incubating and/or analyzing a dispersion of particles , cells or droplets , and/or for performing biochemical reactions with or in such dispersion , said cartridge comprising: 'two oppositely located open ends serving as an inlet and an outlet, respectively,', 'a deformable transparent tube havingsaid tube being adapted to receive a dispersion of particles, cells or droplets, in an interior space of said tube, said interior space being lined by one or several walls of said tube, wherein the tube is configured such that the interior space of said tube, when having received said dispersion, has a circular or oval cross-section, such that the interior space, when the tube is pressed against a surface, has a flat, noncircular cross-section,said cartridge further comprising means to withhold said particles, cells or droplets in said deformable transparent tube, said means to withhold said particles, cells or droplets being located at one or both ends of said tube, said means allowing the passage of liquid through it whilst retaining said particles, cells or droplets.2. The sample cartridge according to claim 1 , wherein said deformable ...

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Номер записи: 92
13-06-2019 дата публикации

CONCENTRATING PARTICLES IN A MICROFLUIDIC DEVICE

Номер: US20190176150A1
Автор: Kapur Ravi, Smith Kyle C., Toner Mehmet
Принадлежит:

A microfluidic device includes: a first microfluidic channel; a second microfluidic channel extending along the first microfluidic channel; and a first array of islands separating the first microfluidic channel from the second microfluidic channel, in which each island is separated from an adjacent island in the array by an opening that fluidly couples the first microfluidic channel to the second microfluidic channel, in which the first microfluidic channel, the second microfluidic channel, and the islands are arranged so that a fluidic resistance of the first microfluidic channel increases relative to a fluidic resistance of the second microfluidic channel along a longitudinal direction of the first microfluidic channel such that, during use of the microfluidic device, a portion of a fluid sample flowing through the first microfluidic channel passes through one or more of the openings between adjacent islands into the second microfluidic channel. 121.-. (canceled)22. A method of changing a concentration of particles within a fluid sample , the method comprising: flowing a fluid sample containing a plurality of a first type of particle into a microfluidic device , wherein the microfluidic device comprises a first microfluidic channel , a second microfluidic channel extending along the first microfluidic channel , and a first array of islands separating the first microfluidic channel from the second microfluidic channel ,wherein the first microfluidic channel, the second microfluidic channel, and the islands are arranged so that a fluidic resistance of the first microfluidic channel increases relative to a fluidic resistance of the second microfluidic channel along a longitudinal direction of the first microfluidic channel such that a portion of the fluid sample flowing through the first microfluidic channel passes through one or more of the openings between adjacent islands into the second microfluidic channel without the first type of particle, andwherein a width ...

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Номер записи: 93
29-06-2017 дата публикации

RAPID AND CONTINUOUS ANALYTE PROCESSING IN DROPLET MICROFLUIDIC DEVICES

Номер: US20170182495A1
Автор: Bakowski Tomasz, Kimmerling Robert, STREY Helmut
Принадлежит:

The compositions and methods described herein are designed to introduce functionalized microparticles into droplets that can be manipulated in microfluidic devices by fields, including electric (dielectrophoretic) or magnetic fields, and extracted by splitting a droplet to separate the portion of the droplet that contains the majority of the microparticles from the part that is largely devoid of the microparticles. Within the device, channels are variously configured at Y- or T junctions that facilitate continuous, serial isolation and dilution of analytes in solution. The devices can be limited in the sense that they can be designed to output purified analytes that are then further analyzed in separate machines or they can include additional channels through which purified analytes can be further processed and analyzed. 117.-. (canceled)18. A method of analyzing an analyte in a sample , the method comprising introducing the sample into a microfluidic device comprising:(a) a substrate comprising a series of contiguous channels and;(b) a field generator,wherein the series of contiguous channels comprises a first channel having an initial segment configured to receive a tagged droplet comprising an analyte bound to a functionalized particle, a middle segment through which the tagged droplet travels, and a terminal segment that bifurcates into a second channel and a third channel,wherein the field generator is positioned adjacent to the first channel and marginalizes the analyte toward a side of the tagged droplet such that, upon reaching the bifurcation, a portion of the tagged droplet that includes the majority of the analyte enters the second channel and the remainder of the tagged droplet enters the third channel, thereby producing, in the second channel, a smaller droplet that contains the majority of the analyte while excluding at least some of the complex mixture.19. A method of manipulating an analyte within a sample , the method comprising:providing the sample ...

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Номер записи: 94
04-06-2020 дата публикации

MIXED-PHASE FLUIDS FOR NUCLEIC ACID SEQUENCING AND OTHER ANALYTICAL ASSAYS

Номер: US20200171498A1
Автор: ALBERNI Julian Sean, ERICKSTAD Michael John, FLEISCHER Chad, MCGINLEY Rebecca, Nemiroski Alex, Oliphant Arnold, VILLARREAL Eric
Принадлежит: OMNIOME, INC.

An analytical system that includes a flow cell, a liquid delivery component, a gas delivery component and a bubble generator component, wherein the liquid delivery component is configured to deliver liquid from one or more reservoirs to the bubble generator component, wherein the gas delivery component is configured to deliver gas from one or more source to the bubble generator component, and wherein the bubble generator component is configured to mix liquids from the liquid delivery component with gas from the gas delivery component to deliver a fluid foam to the inside of the flow cell, wherein the fluid foam includes bubbles of the gas in the liquid. 1. A method for sequencing a nucleic acid , comprising (i) a flow cell comprising a nucleic acid immobilized therein,', '(ii) a bubble generator component that delivers gas bubbles to a liquid at a predefined rate;, '(a) providing a sequencing system comprising'}(b) delivering a series of fluids to the inside of the flow cell to perform a cycle of a sequencing process, wherein at least one of the fluids is a fluid foam produced by the bubble generator component comprising gas bubbles in the liquid; and(c) repeating step (b), thereby determining the sequence for the nucleic acid.2. The method of claim 1 , wherein the nucleic acid is immobilized on a surface inside the flow cell.3. The method of claim 2 , wherein the nucleic acid is present at a site in an array of nucleic acids immobilized on the surface.4. The sequencing system of claim 3 , wherein the sequencing process comprises optically resolving the site from other sites in the array.5. The method of claim 1 , wherein at least one of the fluids that is delivered to the flow cell is fluid foam containing a reversibly terminated nucleotide.6. The method of claim 5 , wherein at least one of the fluids that is delivered to the flow cell is fluid foam containing a deblocking reagent.7. The method of claim 1 , wherein at least one of the fluids that is delivered to ...

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Номер записи: 95
29-06-2017 дата публикации

SYSTEMS FOR HANDLING MICROFLUIDIC DROPLETS

Номер: US20170183722A1
Автор: Link Darren Roy
Принадлежит:

The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel. 118-. (canceled)19. A method of reducing contamination associated with sample handling , comprising:providing an aqueous fluid comprising a sample through a sample inlet;providing an immiscible fluid flowing through a main channel that is in fluidic communication with the sample inlet;partitioning the aqueous fluid with the immiscible fluid to form a plurality of droplets in the main channel, wherein at least one droplet comprises a sample;flowing the droplets toward a downstream separation chamber that is in fluidic communication with the main channel, wherein the separation chamber has a wider cross-section than the main channel cross-section; andseparating the ...

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Номер записи: 96
04-06-2020 дата публикации

INTRACELLULAR DELIVERY USING MICROFLUIDICS-ASSISTED CELL SCREENING (MACS)

Номер: US20200172845A1
Автор: BAKER Charles James, LAI Ghee Chuan, Landgraf Dirk, Okumus Burak, Paulsson Johan
Принадлежит: President and Fellows of Harvard College

Described herein are methods inducing the uptake of an agent by a cell. Aspects of the invention relate to physically compressing the cell to induce perturbations (e.g., holes) in the cell membrane or wall. An agent is taken up by the cell through induced perturbations. 1. A method for uptake of an agent into a cell or population thereof using a microfluidic device , the method comprising:passing a first flow fluid through a flow channel of the microfluidic device, the flow channel being at least partially bounded on a first side by a flexible layer of the microfluidic device and being at least partially bounded on an opposing second side by a rigid layer of the microfluidic device, the first flow fluid including a plurality of a first type of cell therein and a first agent;pressurizing a control channel formed in the flexible layer of the microfluidic device such that the flexible layer physically compresses at least one of the first type of cell in the first flow fluid against the rigid layer to induce at least one temporary perturbation in a membrane of the at least one of the first type of cell; andcausing a portion of the first agent to be taken up by the at least one of the first type of cell through the at least one temporary perturbation in the membrane of the at least one of the first type of cell.2. The method of claim 1 , wherein the causing the portion of the first agent to be taken up includes at least one of:continuing to pass the first flow fluid through the flow channel with the control channel pressurized;adjusting a flow rate of the first flow fluid through the flow channel with the control channel pressurized; orstopping the flow of the first flow fluid through the flow channel with the control channel pressurized.35.-. (canceled)6. The method of claim 1 , wherein the agent is selected from the group consisting of: a DNA staining dye claim 1 , a fluorescent molecule claim 1 , a plasmid claim 1 , a vector claim 1 , a protein claim 1 , a nucleic ...

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Номер записи: 97
04-06-2020 дата публикации

METHOD OF DETERMINING QUALITY OF CELL SEPARATION, PARTICLE DETECTION METHOD, AND PARTICLE SEPARATION APPARATUS

Номер: US20200173980A1
Автор: IJIRI Yuichi, IWANAGA Shigeki, SHIRAI Kentaro, Takahashi Yusuke, YANAGIDA Masatoshi, YOSHIKAWA Keiko
Принадлежит: SYSMEX CORPORATION

A method of determining a quality of a cell separation, according to an aspect, includes: separating, by running a specimen containing a first particle and a second particle in a flow path, the specimen to a first specimen containing the first particle and a second specimen containing the second particle, based on a difference in magnitude of a force exerted on each particle contained in the specimen; measuring first information on an amount of the first specimen and second information on an amount of the second specimen; and determining a quality of the separating based on the first information and the second information. 1. A method of determining a quality of a cell separation , comprising:separating, by running a specimen containing a first particle and a second particle in a flow path, the specimen to a first specimen containing the first particle and a second specimen containing the second particle, based on a difference in magnitude of a force exerted on each particle contained in the specimen;measuring first information on an amount of the first specimen and second information on an amount of the second specimen; anddetermining a quality of the separating based on the first information and the second information.2. The method according to claim 1 , wherein the first specimen in which a presence ratio of the first particle is higher than in the specimen; and', 'the second specimen in which a presence ratio of the first particle is lower than in the specimen., 'the separating comprises separating the specimen to3. The method according to claim 1 , whereinthe separating comprises separating the specimen to the first specimen and the second specimen by running the specimen in a micro flow path.4. The method according to claim 1 , whereinthe separating comprises separating the specimen to the first specimen and the second specimen based on a speed distribution in the flow path.5. The method according to claim 1 , whereinthe separating comprising running the ...

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Номер записи: 98
04-06-2020 дата публикации

METHODS, COMPOSITIONS AND SYSTEMS FOR MICROFLUIDIC ASSAYS

Номер: US20200174005A1
Автор: Chiu Daniel T., NELSON Wyatt, Schiro Perry G., ZHAO Mengxia
Принадлежит:

Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment. 1110-. (canceled)111. A method for identifying a plurality of markers present on an analyte , wherein the method comprises:(a) partitioning a plurality of analytes by flowing the plurality of analytes over a substrate comprising a plurality of micro-cavities or micro-patches, wherein a majority of micro-cavities or micro-patches are configured to contain not more than one analyte and wherein the plurality of micro-cavities or micro-patches are disposed in a microfluidic device;(b) in the plurality of micro-cavities or micro-patches, contacting an analyte of the plurality of analytes with a first structure configured to bind to a first marker, wherein the first structure is connected to a first tag;(c) detecting a signal from the first tag;(d) reducing a level of the signal of the first tag;(e) contacting the analyte with a second structure that binds to a second marker, wherein the second structure is connected to a second tag; and(f) detecting the second tag.112. The method of claim 111 , wherein the contacting of step (b) comprises flowing a fluid comprising the first structure through a channel that is in fluid communication with the plurality of micro-cavities or micro-patches.113. The method of claim 111 , wherein claim 111 , following the contacting step of step (b) claim 111 , the method further comprises: contacting the analyte with a wash buffer.114. The method of claim 112 , wherein the method is performed on a plurality of ...

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Номер записи: 99
15-07-2021 дата публикации

PARTICLE SEPARATION

Номер: US20210213451A1
Автор: Chen Chien-Hua, Shkolnikov Viktor
Принадлежит: Hewlett-Packard Development Company, L.P.

A fluid entrained particle separator may include an inlet passage to direct particles entrained in a fluid, a first separation passage branching from the inlet passage, a second separation passage branching from the inlet passage and electrodes to create electric field exerting a dielectrophoretic force on the particles to direct the particles to the first separation passage or the second separation passage, wherein the first separation passage, the second separation passage, the electric field and the dielectrophoretic force extend in a plane. 1. A fluid entrained particle separator comprising:an inlet passage to direct particles entrained in a fluid;a first separation passage branching from the inlet passage;a second separation passage branching from the inlet passage;electrodes to create electric field exerting a dielectrophoretic force on the particles to direct the particles to the first separation passage or the second separation passage, wherein the first separation passage, the second separation passage, the electric field and the dielectrophoretic force extend in a plane.2. The fluid entrained particle separator of further comprising a particle focuser to focus the particles into a laminar flow within the inlet passage.3. The fluid entrained particle separator of claim 2 , wherein the particle focuser comprises a hydrodynamic focuser.4. The fluid entrained particle separator of claim 3 , wherein the hydrodynamic particle focuser comprises a first sheath flow passage leading to the inlet passage and a second sheath flow passage leading to the inlet passage and a particle flow passage leading to the inlet passage claim 3 , the particle flow passage to supply particles between sheath fluid from the first sheath flow passage and the second sheath flow passage.5. The fluid entrained particle separator of claim 2 , wherein the particle focuser is selected from a group of particle focusers consisting of a free flow negative dielectrophoresis particle focuser and a ...

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Номер записи: 100