16-05-2013 дата публикации
Номер: US20130122598A1
Принадлежит:
The invention relates to a process for the specific isotopic labeling of Valine, Leucine and Isoleucine amino acids. The process of the invention uses a 2-alkyl-2-hydroxy-3-oxobutanoic acid in which the alkyl substituent in position 2 is ethyl or methyl. The invention can be used for the analysis of proteins, in particular by NMR. 2. The process of further comprising:b) overexpression of the protein by the bacteria in the medium, andc) purification of the protein.3. The process of wherein:{'sup': '1', 'Ris selected from the group consisting of{'sup': 12', '12', '13', '13', '13', '13, 'sub': 3', '3', '3', '3', '2', '2, 'CH, CD, CH, CD, CHD, and CHD,'}{'sup': '2', 'Ris selected from the group consisting of{'sup': 12', '12', '13', '13', '13', '13', '12', '12', '12', '12', '13', '12', '13', '13', '13', '13', '13', '13', '13', '13', '13', '12', '13', '12, 'sub': 3', '3', '3', '3', '2', '2', '3', '2', '3', '2', '3', '2', '3', '2', '3', '2', '2', '2', '2', '2', '2', '2', '2', '2, 'CH, CD, CH, CD, CHD, CHD, CHCD, CDCD, CHCD, CHCD, CDCD, CHDCD, CHDCD, CHDCD, and CHDCD.'}4. The process of claim 1 , wherein the acetolactate compound is selected from the group consisting of:{'sup': 13', '2, 'sub': '3', '2-hydroxy-2-(C)methyl-3-oxo-4(H)butanoic acid (formula 4),'}{'sup': 2', '13, 'sub': '3', '2-hydroxy-2-(H)methyl-3-oxo-4(C)butanoic acid (formula 5),'}{'sup': 2', '13, 'sub': '5', '2-(H)ethyl-2-hydroxy-3-oxo-4-(C)methylbutanoic acid (formula 6),'}{'sup': 13', '2, 'sub': '5', '1,2,3,4-(C)-2-(H)ethyl-2-hydroxy-3-oxobutanoic acid (formula 9),'}{'sup': 13', '13', '2, 'sub': '3', '1,2,3-(C)-2-(C)methyl-2-hydroxy-3-oxo-4-(H)butanoic acid (formula 21),'}{'sup': 13', '2, 'sub': '3', '1,2,3,4-(C)-2-(H)methyl-2-hydroxy-3-oxobutanoic acid (formula 22),'}{'sup': 13', '2', '13', '2, 'sub': 2', '2', '3, '1,2,3-(C)-2-(1′-(H),C)ethyl)-2-hydroxy-3-oxo-4-(H)butanoic acid (formula 24),'}3,4-(13C)-2-(13C)methyl-2-hydroxy-3-oxo-4-(2H3)butanoic acid (formula 36),3,4-(13C)-2-(2H3,13C)methyl-2-hydroxy-3 ...
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