27-02-2020 дата публикации
Номер: US20200062719A1
Автор:
Xinlai Cheng,
Stefan Wolfl,
Ralf Mrowka,
James Adjaye,
CHENG XINLAI,
WOLFL STEFAN,
MROWKA RALF,
ADJAYE JAMES,
Cheng, Xinlai,
Wolfl, Stefan,
Mrowka, Ralf,
Adjaye, James
A method of producing a pluripotent stem cell is provided. The method is comprising contacting a non-pluripotent donor cell obtained from a mammalian donor with a compound characterized by general formulas (1) and (3). Furthermore, methods for inducing OCT4 and NANOG, increasing histone 3 lysine methylation and the maintenance of pluripotency are provided. 3. The method according to claim 1 , wherein{'sup': I', 'a', '1', '2', '3', '4', 'o', 'm', 'p', 'm′', 'o′, 'sub': 3', '2, 'each R, R, R, R, R, R, R, R, R, R and R is independently selected from —H, —CH, —F, —Cl, —Br, —I, —OH, —NO, —CN or —OMe.'}4. The method according to claim 1 , wherein{'sup': '1', 'sub': '3', 'Ris selected from —CH, and —H;'}{'sup': '2', 'sub': '3', 'Ris selected from —CH, and —H;'}{'sup': '3', 'sub': '3', 'Ris selected from —CH, —H, —F, —Cl, and —Br;'}{'sup': '4', 'sub': '3', 'Ris selected from —CH, and —H;'}{'sup': o', 'o′, 'sub': '2', 'Rand R are selected from —NO, —F, and —H;'}{'sup': 'p', 'sub': 3', '2, 'Ris selected from —CH, —F, —Cl, —Br, —NO, —OMe, —OH, —CN, and —H;'}{'sup': m', 'm′, 'sub': '2', 'Rand R are selected from —Cl, —H, —F, —OH, —NO, and —OMe, and/or'}{'sup': 'z', 'Ris selected from —H and formula (2).'}5. The method according to claim 1 , wherein the compound according to formula (1) isa) N,N-dimethyl-2-(6-methyl-2-p-tolylimidazo[1,2-a]pyridine-3-yl)acetamid (100),b) 6-methyl-2-(p-tolyl)imidazo[1,2-a]pyridine (101),c) 2-(4-fluorophenyl)-6-methyl-imidazo[1,2-a]pyridine (102),d) 2-(4-chlorophenyl)-6-methyl-imidazo[1,2-a]pyridine (103),e) 2-(4-bromophenyl)-6-methyl-imidazo[1,2-a]pyridine (104),f) 6-methyl-2-(4-nitrophenyl)imidazo[1,2-a]pyridine (105),g) 2-(4-methoxyphenyl)-6-methyl-imidazo[1,2-a]pyridine (106),h) 4-(6-methylimidazo[1,2-a]pyridin-2-yl)phenol (107),i) 4-(6-methylimidazo[1,2-a]pyridin-2-yl)benzonitrile (108),j) 6-methyl-2-phenyl-imidazo[1,2-a]pyridine (109),k) 6-methyl-2-(3-nitrophenyl)imidazo[1,2-a]pyridine (110),l) 6-methyl-2-(2-nitrophenyl)imidazo[1,2-a]pyridine ...
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