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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 2729. Отображено 100.
23-02-2012 дата публикации

Conjugates of hydroxyalkyl starch and a protein, prepared by native chemical ligation

Номер: US20120046235A9
Автор: Nobert Zander, Ronald Frank
Принадлежит: Individual

Conjugates of an active substance and hydroxyalkyl starch (HAS) are provided herein. The active substance and the HAS are linked by a chemical moiety having a structure according to formula (I) wherein Y is O or S, and X is SH or (F). The conjugate has a structure according to formula (IV) wherein HAS′ is a residue of HAS or a derivative thereof linked to the thioester group, and AS′ is a residue of the active substance or derivative thereof linked to the alpha-X beta-amino group; or a structure according to formula (V) wherein HAS′ is a residue of HAS or derivative thereof linked to the alpha-X beta-amino group, and AS′ is a residue of the active substance or derivative thereof linked to the thioester group, and wherein the group —(C═Y) is derived from the thioester group —(C═Y)—S—R′ and the group HN—CH—CH 2 —X is derived from the alpha-X beta amino group.

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Номер записи: 1
23-02-2012 дата публикации

Coating Comprising An Elastin-Based Copolymer

Номер: US20120046640A1
Автор: Lothar W. Kleiner, Syed F.A. Hossainy, Yiwen Tang
Принадлежит: Abbott Cardiovascular Systems Inc

The present invention is directed to medical devices including coatings. The coatings include a topcoat which includes a copolymer comprising a block of an elastin pentapeptide. The topcoat is over a layer of poly(vinyl alcohol) on a hydrophobic coating or over a porous coating comprising pores or depots that include a bioactive agent.

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Номер записи: 2
15-03-2012 дата публикации

Methods of processing recombinant procollagen

Номер: US20120065376A1
Автор: Amit Yaari, Hanan Stein, Michal Rosenthal, Oded Shoseyov, Or Dgany, Tamar Haya Tal
Принадлежит: Collplant Ltd

A method of generating atelocollagen is disclosed. The method comprises contacting a human telopeptide-comprising collagen with a protease selected from the group consisting of neutrase, subtilisin, ficin recombinant human trypsin and recombinant human pepsin, wherein said human telopeptide-comprising collagen is expressed in a non-animal cell, thereby generating the atelocollagen. Compositions comprising the atelocollagen generated thereby are also disclosed.

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Номер записи: 3
07-06-2012 дата публикации

Implantable material and a method for the preparation thereof

Номер: US20120142898A1
Автор: Andrew Michael Collins, Christopher Holland, David Philip Knight, Nicholas James Vavasour Skaer, Tom Louis Dirk Gheysens
Принадлежит: Individual

A method for the preparation of a regenerated silk fibroin solution comprises the steps of: treating silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C.; and subsequently degumming the treated silk or silk cocoons; or alternatively, degumming silk or silk cocoons; and subsequently treating the degummed silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C. The invention also extends to fibroin solution, a fibroin material and an implant useful for cartilage repair.

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Номер записи: 4
26-07-2012 дата публикации

Macroporous Microcarrier Specific to Liver Cell, Preparation Method and Use Thereof

Номер: US20120190113A1
Автор: Duhui Gong, Huancheng Zhou, Mingxin Pan, YI Gao, Zhi Zhang, Zhiwei Hu
Принадлежит: Individual

The present invention provides a macroporous microcarrier specific to hepatocytes using silk fibroin and galactosylated chitosan as main raw material, a preparation method thereof, and application for hepatocyte culture under the culture condition of microgravity rotation. The macroporous microcarrier s a sphere prepared from silk fibroin and galactosylated chitosan under the effect of crosslinker, wherein based on the total weight of the sphere, the content of silk fibroin is 50-80 wt % and the content of galactosylated chitosan is 15-40 wt %. The diameter of the microcarrier is 200-500 μm, and the aperture of the microcarrier is 40-80 μm. Compared with normal solid scaffold material, the microcarrier provided by the present invention has larger surface area/volume ratio and, a sinus gap structure extremely similar with in-vivo liver sinus structure, therefore it is more conducive to adhering of the hepatocytes on the scaffold material, contacting between cells, transporting oxygen and nutrient components and excreting metabolic products.

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Номер записи: 5
04-10-2012 дата публикации

Methods of Modifying Agricultural Co-Products and Products Made Therefrom

Номер: US20120252935A1
Автор: Barry L. Mcgraw, Ramanathan S. Lalgudi, Robert J. Cain
Принадлежит: Battelle Memorial Institute Inc

In a method of producing a polymer composite, a polymer is provided in a liquid state such as a molten state. A plant material, such as soymeal, is provided that includes protein and carbohydrate. A reactive protein denaturant is also provided. A dispersion of the plant material and the reactive protein denaturant is formed in a matrix of the liquid polymer. The plant material is reacted to bond with the reactive protein denaturant, and the reactive protein denaturant is reacted to bond with the polymer. The polymer is solidified to produce the polymer composite.

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Номер записи: 6
18-10-2012 дата публикации

Coating Comprising An Elastin-Based Copolymer

Номер: US20120263759A1
Автор: Lothar W. Kleiner, Syed F.A. Hossainy, Yiwen Tang
Принадлежит: Abbott Cardiovascular Systems Inc

A copolymer comprising a block of an elastin pentapeptide and method of making and using the copolymer are provided. The copolymer may be used as a coating on a stent. Methods of using a stent coated with the copolymer are also provided.

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Номер записи: 7
01-11-2012 дата публикации

Metal-polysaccharide conjugates: compositions, synthesis and methods for cancer therapy

Номер: US20120277409A1
Автор: Chih-Wei Hsu, David J. Yang, Hsien-Fan Chen, Ning Tsao, Yen-Shian Wu, Yun-Chi Lo
Принадлежит: Taiwan Hopax Chemicals Manufacturing Co Ltd, University of Texas System

The current disclosure, in one embodiment, includes a polysaccharide conjugate. This conjugate has a polysaccharide and at least one liner covalently bound to the polysaccharide. The conjugate also has at least one metal conjugated by said linker. According to another embodiment, the disclosure provides a method of synthesizing a polysaccharide conjugate by covalently bonding a linker to a polysaccharide to obtain an intermediate and by conjugating said intermediate to a metal to form a polysaccharide conjugate. This conjugate has a higher relaxivity, so it is suitable to be used as a contrast medium for hybrid camera.

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Номер записи: 8
20-12-2012 дата публикации

Crosslinked Hydrogels and Related Method of Preparation

Номер: US20120321585A1
Автор: Fengfu Li, Jae-Il Ahn, May Griffith
Принадлежит: OTTAWA HEALTH RESEARCH INSTITUTE, UNIVERSITY OF OTTAWA

The present invention provides a method of manufacturing a hydrogel comprising the step of crosslinking a biopolymer using a carbodiimide crosslinker of Formula I wherein at least one of R 1 and R 2 is a functional group that is a bulky organic functional group. R 1 and R 2 can each independently be an optionally substituted saturated or unsaturated functional group selected from the group consisting of an alkyl, a cycloalkyl, a heterocyclic, and an aryl. The bulky organic functional group will slow down the crosslinking reaction of carbodiimide due to the steric effects and/or electronic effects, in comparison to a crosslinking reaction using EDC. Also provided are the hydrogels and ophthalmic devices prepared using the method of the invention and uses thereof.

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Номер записи: 9
20-12-2012 дата публикации

Modified recombinant factor viii and von willebrand factor and methods of use

Номер: US20120322737A1
Автор: Hans-Peter Schwarz, Peter Turecek
Принадлежит: Baxter Healthcare SA, Baxter International Inc

The present invention provides novel methods of increasing the survival of a coagulation protein by inhibiting the interaction with a clearance receptor. The invention also provides methods of preparing compositions that inhibit coagulation protein clearance receptors. Conjugated coagulation proteins, including compositions and formulations thereof, are also provided by the present invention.

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Номер записи: 10
27-12-2012 дата публикации

Cross-linked compositions

Номер: US20120329132A1
Автор: Chagai Komlos, Ishay Attar, Meir Haber, Natalie Iram, Orahn Preiss Bloom
Принадлежит: Lifebond Ltd

Improved compositions comprising a cross-linkable protein or polypeptide, and a non-toxic material which induces cross-linking of the cross-linkable protein. The compositions are optionally and preferably prepared in a non-phosphate buffer solvent. Optionally and preferably, the cross-linkable protein includes gelatin and any gelatin variant or variant protein as described herein. Optionally and preferably, the non-toxic material comprises transglutaminase (TG), which may optionally comprise any type of calcium dependent or independent transglutaminase, which may for example optionally be a microbial transglutaminase (mTG).

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Номер записи: 11
31-01-2013 дата публикации

Cosmetic sponge and method for providing a cosmetic sponge

Номер: US20130028949A1
Автор: Wayne Celia
Принадлежит: DIVERSIFIED GLOBAL TECHNOLOGIES LLC

This invention relates to a cosmetic sponge and a method of providing a cosmetic sponge incorporating a non-denatured collagen and other additives for skin care, cleaning, and cosmetic application.

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Номер записи: 12
14-02-2013 дата публикации

Phase separated composite

Номер: US20130041044A1
Автор: Joo Eun Chung, Li Shan Wang, Motoichi Kurisawa
Принадлежит: Agency for Science Technology and Research Singapore

A composite is disclosed. The composite comprises a first conjugate of a polymer and a first phenol-containing moiety, and a second conjugate of a gelatin or collagen and a second phenol-containing moiety, wherein the polymer is selected so that the first conjugate is less cell-adhesive than the second conjugate, at least one of the first and second conjugates is crosslinked to form a matrix, and the composite comprises discrete regions that are rich in one of said first and second conjugates. A method of forming such composite is also disclosed. The method comprises mixing precursors for the first and second conjugates in a solution for forming said composite, and dispersing a catalyst in the solution to catalyze crosslinking of at least one of the first and second conjugates to form the matrix. The composite may be used to grow cells.

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Номер записи: 13
09-05-2013 дата публикации

Methods of making hyaluronic acid/collagen compositions

Номер: US20130116411A1
Автор: Jacob F. Pollock, Nicholas J. Manesis, Xiaojie Yu
Принадлежит: Allergan Inc

Hyaluronic acid and collagen may be crosslinked in aqueous solution as described herein. The crosslinked macromolecular matrices obtained in this process may be used as a hydrogel for implants and fillers for human aesthetic and therapeutic products.

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Номер записи: 14
04-07-2013 дата публикации

Isolation and deglycosylation of glycoproteins

Номер: US20130171658A1
Автор: Scott P. Fulton, Steven M. Murphy
Принадлежит: ProZyme Inc

The invention provides more rapid and cost-effective methods of deglycosylating target glycoproteins. In methods of the invention, the target glycoprotein is isolated from initial samples, which may contain multiple other glycoproteins, by subjecting the initial sample to a solid phase containing an affinity ligand, such as a deglycosylated antibody, that interacts specifically with the target glycoprotein. Once separated from the sample, the target glycoprotein can be deglycosylated in situ, or eluted from the solid phase, quantitated, and then deglycosylated.

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Номер записи: 15
08-08-2013 дата публикации

CONVERSION OF CORN GLUTEN MEAL INTO A SOLID ARTICLE THROUGH THE USE OF A NON-TOXIC ADDITIVE

Номер: US20130203881A1
Автор: Maron Odelia, WOERDEMAN Dara L.
Принадлежит:

Disclosed are fast-curing, inexpensive corn-gluten resin compositions, methods for making them, methods for forming them into solid articles. In some embodiments, the resin composition includes corn meal gluten and a non-toxic organic acid. 1. A resin composition for molding formed articles comprising: about 70% to about 99% w/w corn meal gluten,', 'about 1% to about 18% organic acid, and', 'an optional solvent., 'a resin component itself comprising by weight of the resin component2. The resin composition of further comprising a reinforcement/filler component claim 1 , the reinforcement/filler component comprising up to about 40% by weight of the resin composition.3. The composition of claim 1 , wherein the organic acid is selected from lactic acid claim 1 , citric acid claim 1 , sorbic acid claim 1 , malic acid claim 1 , succinic acid claim 1 , and maleic acid.4. The resin composition of claim 1 , comprising at least about 98% corn meal gluten by weight of the resin component.5. The resin composition of claim 1 , comprising at least about 97% corn meal gluten by weight of the resin component.6. The resin composition of claim 1 , comprising at least about 92.5% corn meal gluten by weight of the resin component.7. The resin composition of claim 1 , comprising at least about 90% corn meal gluten by weight of the resin component.8. The resin composition of claim 1 , comprising at least about 87.5% corn meal gluten by weight of the resin component.9. The composition of claim 1 , wherein said reinforcement/filler component is selected from natural fibers claim 1 , sawdust claim 1 , inorganic fibers claim 1 , inorganic particles claim 1 , foaming agents claim 1 , clays claim 1 , zeolites claim 1 , and combinations thereof.10. A formed article comprising: about 70% to about 99% w/w corn meal gluten,', 'about 1% to about 18% organic acid, and', 'an optional solvent., 'a resin component itself comprising by weight of the resin component, 'a resin composition11. The formed ...

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Номер записи: 16
22-08-2013 дата публикации

METHODS FOR PREPARING PEG-HEMOGLOBIN CONJUGATES USING REDUCED REACTANT RATIOS

Номер: US20130217860A1
Автор: Malavalli Ashok, Vandegriff Kim D.
Принадлежит: SANGART, INC.

The present invention relates generally to methods for preparing polyethylene glycol (“PEG”) conjugated hemoglobin (“Hb”) using reduced reactant ratios. More specifically, the present invention relates to methods for preparing PEG conjugated Hb (“PEG-Hb”) with enhanced yield and purity. 1. A method for preparing polyethylene glycol conjugated hemoglobin (PEG-Hb) comprising the steps of:a) mixing hemoglobin (Hb) with 2-iminothiolane (2-IT) in an aqueous diluent, wherein the 2-IT is at a concentration of between 7 and 8 molar excess in the diluent over the Hb concentration, to form thiolated Hb; andb) adding polyethylene glycol (PEG)-maleimide (Mal) to the thiolated Hb in the aqueous diluent, wherein the PEG-Mal is at a concentration of between 9 and 15 molar excess in the diluent over the Hb concentration to form a PEG-Hb conjugate, wherein the PEG-Mal has an average molecular weight of between 4,000 and 6,000 daltons (Da);wherein the PEG-Hb conjugate contains an average of between 7.1 and 8.9 PEG molecules per Hb; andwherein the PEG-Hb conjugate has a tighter molecular weight distribution than a PEG-Hb conjugate prepared using a higher molar ratio of 2-IT or PEG-Mal.2. The method according to claim 1 , wherein the 2-IT is at a concentration of 7.5 molar excess in the diluent over the Hb concentration.3. The method according to claim 1 , wherein the PEG-Mal is at a concentration of 12 molar excess in the diluent over the Hb concentration.4. The method according to claim 1 , wherein the PEG-Mal has an average molecular weight of 5 claim 1 ,000 Da.5. The method according to claim 1 , wherein the PEG-Hb conjugate has a partial pressure of oxygen at which the Hb is 50% saturated (p50) less than native stroma free hemoglobin from an equivalent source when measured under essentially identical conditions.6. The method according to claim 5 , wherein the p50 of the PEG-Hb conjugate is less than 10 millimeters of mercury (mmHg).7. The method according to claim 5 , wherein the ...

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Номер записи: 17
20-03-2014 дата публикации

Silk Protein Composite Coating Solution and its Preparation Method and Application

Номер: US20140076195A1
Автор: Yaoqi Jin
Принадлежит: Individual

Silk protein composite coating solution and its preparation method and application. In weight percent, silk protein complex coating liquid is constituted by the following substances: 2 to 10% of the crosslinking agent, 0.5 to 2% acetic acid, 10 to 20% of the silk protein, the remainder being water. The method for preparation of silk protein composite coating solution has these steps: (1) preparation of the silk protein; (2) silk protein composite coating solution was prepared. Silk protein of the present invention has a natural emollient, antibacterial, and anti-allergic effects, and can be used on daily sanitary supplies for women, infants, and elderlies.

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Номер записи: 18
06-01-2022 дата публикации

Bioadhesive for Soft Tissue Repair

Номер: US20220001074A1
Автор: Ahmad KHEIRKHAH, Ehsan Shirzaei Sani, Nasim Annabi, Reza Dana
Принадлежит: Northeastern University Boston, Schepens Eye Research Institute Inc

The present invention provides compositions and methods for repair and reconstruction of defects and injuries to soft tissues. Some aspects of the invention provide tissue adhesives comprising a hybrid hydrogel by using a naturally derived polymer, gelatin and a synthetic polymer, polyethylene glycol, wherein the hydrogel is biocompatible, biodegradable, transparent, strongly adhesive to corneal tissue, and have a smooth surface and biomechanical properties similar to the cornea.

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Номер записи: 19
05-01-2017 дата публикации

MODIFIED BIOPOLYMERS AND METHODS OF PRODUCING AND USING THE SAME

Номер: US20170002098A1
Автор: Ayoub Ali, Bray James Charles, Chan Ryan Nicholas
Принадлежит:

Modified biopolymers, such as, charge-modified biopolymers, cross-linked biopolymers, and cross-linked, charge-modified biopolymers are provided along with methods of producing and using the same. 1. A method for producing a cross-linked , charge-modified biopolymer comprising:combining a biopolymer and at least one charge-modifying agent to form a homogenous reaction blend;reacting the biopolymer and the at least one charge-modifying agent in the homogenous reaction blend; andcross-linking the biopolymer in the homogeneous reaction blend to form a cross-linked, charge-modified biopolymer.2. The method of claim 1 , wherein the combining step further comprises combining a plasticizer and optionally a catalyst with the biopolymer and the at least one charge-modifying agent to form the homogenous reaction blend.3. The method of claim 1 , wherein the cross-linking step further comprises reacting the charge-modified biopolymer with at least one cross-linking agent claim 1 , optionally in the presence of an initiator.4. The method of claim 1 , wherein the reacting and cross-linking steps occur simultaneously.5. The method of any one of claim 1 , further comprising foaming the cross-linked claim 1 , charge-modified biopolymer.6. The method of claim 1 , wherein the cross-linked claim 1 , charge-modified biopolymer comprises a plurality of void spaces formed therein having an average diameter of about 0.1 to about 500 microns.7. The method of claim 1 , wherein the biopolymer comprises at least two different biopolymers claim 1 , optionally wherein one of the at least two different biopolymers is a charge-modified biopolymer.8. The method of claim 1 , wherein the cross-linked claim 1 , charge-modified biopolymer has a net positive charge or a net negative charge.9. The method of claim 1 , wherein the cross-linked claim 1 , charge-modified biopolymer is a polyampholyte.10. The method of claim 1 , wherein the combining step comprises melting blending the biopolymer and the at ...

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Номер записи: 20
03-01-2019 дата публикации

Silk Performance Apparel and Products and Methods of Preparing the Same

Номер: US20190003113A1
Автор: Altman Gregory H., Mortarino Enrico
Принадлежит:

Silk infused performance apparel and methods of preparing the same are disclosed herein. In some embodiments, silk performance apparel includes textiles, fabrics, consumer products, leather, and other materials that are coated with aqueous solutions of pure silk fibroin-based protein fragments. In some embodiments, coated apparel products, textiles, and upholstery, as well as other materials, exhibit surprisingly improved moisture management properties, resistance to microbial growth, increased abrasion resistance, and flame resistance. 1. A method of coating a material with silk fibroin that comprises silk-based proteins or fragments thereof to provide a silk fibroin coated material , wherein the silk fibroin coated upon the silk fibroin coated material is heat resistant to a selected temperature , the method comprising:(a) preparing a silk fibroin solution comprising a concentration of one or more of low molecular weight silk fibroin, medium molecular weight silk fibroin, and high molecular weight silk fibroin at less than about 1% by volume (v/v);(b) coating a surface of the material with the silk fibroin solution; and(c) drying the surface of the material that has been coated with the silk fibroin solution to provide the silk fibroin coated material, wherein drying the surface of the material comprises heating the surface of the material without substantially modifying silk fibroin coating performance.2. The method of claim 1 , wherein the silk fibroin solution comprises low molecular weight silk fibroin.3. The method of claim 1 , wherein the silk fibroin solution comprises medium molecular weight silk fibroin.4. The method of claim 1 , wherein the step of preparing the silk fibroin solution comprises adding a chemical fabric softener to the silk fibroin solution.5. The method of claim 1 , wherein the silk fibroin solution comprises a comprises a Bronsted acid.6. The method of claim 1 , wherein the silk fibroin solution comprises one or more of citric acid and ...

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Номер записи: 21
15-01-2015 дата публикации

Temperature-responsive polymer particles in protein separation applications

Номер: US20150018440A1
Автор: Brad William Woonton, Kirthi De Silva, Milton Thomas William Hearn, Pankaj Maharjan, William Roy Jackson
Принадлежит: Commonwealth Scientific and Industrial Research Organization CSIRO, MONASH UNIVERSITY

The present invention relates to a method for isolating proteins from a solution containing the proteins. The invention also relates to a method for the chromatographic separation of proteins. The present invention also relates to crosslinked hydroxylic polymer particles functionalized with temperature-responsive copolymer, and to methods of preparing such particles.

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Номер записи: 22
10-02-2022 дата публикации

Photoactivated crosslinking of a protein or peptide

Номер: US20220040371A1
Автор: Alan George Brownlee, Charles Mark Lindall, Christopher Malcolm Elvin, Jerome Anthony Werkmeister, John Alan Maurice Ramshaw
Принадлежит: Cook Medical Technologies LLC

A method of crosslinking a protein or peptide for use as a biomaterial, the method comprising the step of irradiating a photoactivatable metal-ligand complex and an electron acceptor in the presence of the protein or peptide, thereby initiating a cross-linking reaction to form a 3-dimensional matrix of the biomaterial.

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Номер записи: 23
30-01-2020 дата публикации

Adhesive compositions made from plant protein and phenolics

Номер: US20200032121A1
Автор: Gudrun Schmidt, Jonathan James Wilker
Принадлежит: PURDUE RESEARCH FOUNDATION

The present disclosure relates to adhesive compositions made from the reaction product of plant protein and phenolics, and method of making and using the adhesive compositions. A maximum bonding strength of 7.8±1.5 MPa was obtained using only 1 weight % tannic acid in a zein matrix of pH=7 and cured at 120° C.

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Номер записи: 24
04-02-2021 дата публикации

SYNTHETIC, MUCUS-LIKE HYDROGEL AND METHOD OF PREPARATION, AND SYSTEM AND METHOD FOR PERFORMING MICRORHEOLOGY ON HYDROGELS AND OTHER COMPLEX FLUIDS

Номер: US20210032411A1
Автор: DUNCAN Gregg, Hawkins Robert, Joyner Katherine, Song Bongsub Daniel
Принадлежит:

A synthetic hydrogel is described, including hydrated mucin glycoproteins cross-linked with multi-arm thiol functional cross-linker, which can be prepared to model viscoelastic and micro-rheological properties of natural mucus. Such synthetic hydrogel can be prepared from a wide variety of mucin raw materials. Also described is a method of microrheologically characterizing mucus, by dispersing in the mucus muco-inert particles (MIP), irradiating the mucus containing MIP with polarized light, and measuring fluorescence polarization (FP) resulting from rotational diffusion of the MIP in the mucus in response to such irradiating, as a microrheological characteristic of the mucus. This method can be carried out using a plate reader equipped with a spectrofluorometer and polarized filter set, and therefore can be readily carried out in clinical settings without the necessity of specialized microrheological equipment. 1. A synthetic hydrogel , comprising hydrated mucin glycoproteins cross-linked with multi-aim thiol functional cross-linker.2. The synthetic hydrogel of claim 1 , wherein the multi-arm thiol functional cross-linker comprises thiol functionality at the termini of multiple ones of its arms.3. The synthetic hydrogel of claim 1 , wherein the multi-arm thiol functional cross-linker has four arms claim 1 , each of which is linked at a central organic core structure and extends outwardly therefrom claim 1 , and comprises polyalkyloxy linear segments and a terminal thiol functionality.4. The synthetic hydrogel of claim 1 , wherein each arm of the multi-armor thiol functional cross-linker comprises a chain structure of the formula —O(CHCHO)CHCHSH wherein n is in a range of from 1 to 1000.5. The synthetic hydrogel of claim 1 , wherein the mucin glycoproteins are porcine mucin glycoproteins claim 1 , bovine mucin glycoproteins claim 1 , or human mucin glycoproteins.7. The synthetic hydrogel of claim 6 , wherein the mucin glycoproteins are porcine mucin glycoproteins or ...

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Номер записи: 25
24-02-2022 дата публикации

PROGRAMMING PROTEIN POLYMERIZATION WITH DNA

Номер: US20220056220A1
Автор: Hayes Oliver G., McMillan Janet R., Mirkin Chad A.
Принадлежит:

The present disclosure is generally directed to methods for making protein polymers. The methods comprise utilizing oligonucleotides for controlling the association pathway of oligonucleotide-functionalized proteins into oligomeric/polymeric materials. 1. A method of making a protein polymer comprising contacting:(a) a first protein monomer comprising a first protein to which a first oligonucleotide is attached, the first oligonucleotide comprising a first domain (V) and a second domain (W); and(b) a second protein monomer comprising a second protein to which a second oligonucleotide is attached, the second oligonucleotide comprising a first domain (V′) and a second domain (W′),wherein (i) V is sufficiently complementary to V′ to hybridize under appropriate conditions and (ii) W is sufficiently complementary to W′ to hybridize under appropriate conditions, and wherein the contacting results in V hybridizing to V′,thereby making the protein polymer.2. The method of claim 1 , wherein the contacting allows W to hybridize to W′.3. The method of or claim 1 , wherein the first protein and the second protein are the same.4. The method of or claim 1 , wherein the first protein and the second protein are different.5. The method of any one of - claim 1 , wherein the first protein and the second protein are subunits of a multimeric protein.6. The method of any one of - claim 1 , wherein the first oligonucleotide is attached to the first protein via a lysine or cysteine on the surface of the first protein.7. The method of any one of - claim 1 , wherein the first oligonucleotide is DNA claim 1 , RNA claim 1 , a combination thereof claim 1 , or a modified form thereof.8. The method of any one of - claim 1 , wherein V is from about 10-100 nucleotides in length.9. The method of any one of - claim 1 , wherein W is from about 10-100 nucleotides in length.10. The method of any one of - claim 1 , wherein the second oligonucleotide is attached to the second protein via a lysine or ...

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Номер записи: 26
03-03-2022 дата публикации

Methods and Compositions for Maintaining the Conformation and Structural Integrity of Biomolecules

Номер: US20220062370A1
Автор: Patel Alpeshkumar P., Seifert Jennifer, Sood Paul R., Zarzycki Ryszard
Принадлежит:

A liquid ink composition includes a liquid phase and particles suspended in the liquid phase, the particles containing a target pharmaceutical or biological agent. The biological activity of the target pharmaceutical or biological agent is preserved upon suspension of the particles in the liquid phase. The liquid phase is capable of solidifying via a solidification process. 1. A liquid ink composition comprising:a liquid phase andparticles suspended in the liquid phase, the particles containing a pharmaceutical or biological agent,wherein the biological activity of the pharmaceutical or biological agent is preserved upon suspension of the particles in the liquid phase,wherein the liquid phase is capable of solidifying to form three dimensional structures.2. The liquid ink composition of claim 1 , the particles being formed by a process comprising: a pharmaceutical or a biological agent; and', 'a substrate that is soluble in the solution, comprising one or more chemical species;, 'preparing a solution, formed from water as a solvent, comprisingcombining the solution with an oil phase to form a water-in-oil emulsion in which the solution is dispersed in the oil phase;lyophilizing the emulsion,wherein the particles are formed prior to or simultaneously with the lyophilizing,wherein the pharmaceutical or biological agent is entrapped by the formed particles,wherein the substrate composition and the oil phase are selected so that the particles formed are suspendable in the liquid ink composition and the biological activity of the pharmaceutical or biological agent is preserved upon suspension of the particles in the liquid ink composition, andwherein one or more substances selected from the group consisting of a surfactant, a stabilizer, an emulsifier, and combinations thereof are incorporated as part of the substrate.3. The liquid ink composition of claim 1 , wherein the biological activity of the pharmaceutical or biological agent is preserved upon solidification of ...

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Номер записи: 27
23-02-2017 дата публикации

PRODUCTION OF PROTEIN-POLYSACCHARIDE CONJUGATES

Номер: US20170049141A1
Автор: Damodaran Srinivasan, Lucey John A., Zhu Dani
Принадлежит: WISCONSIN ALUMNI RESEARCH FOUNDATION

The present invention provides novel compositions and methods for producing protein-polysaccharide conjugates in aqueous solutions. Also provided are methods for limiting the Maillard reaction to the very initial stage, the formation of the Schiff base. Provided are methods to obtain a simple product of Schiff base with white color, and compositions obtained using the methods of the present invention. 1. A method of preparing a polysaccharide-protein conjugate , comprising reacting a polysaccharide comprising a reducing sugar and a protein hydrolysate in an aqueous solution under temperature conditions of from about 40° C. to about 120° C. , thereby producing a polysaccharide-protein conjugate.2. The method of claim 1 , wherein the solution is acidified to a pH of from about 6.0 to about 8.0.3. The method of claim 1 , wherein the reducing sugar and protein hydrolysate are reacted under temperature conditions of from about 40° C. to about 120° C. for a period of from about 1 hour to about 48 hours.4. The method of claim 1 , further subjecting the solution to a hydrostatic pressure in the amount of 1-20 MPa.5. The method of claim 1 , further comprising the step of recovering the polysaccharide-protein conjugate from the solution.6. The method of claim 1 , wherein the polysaccharide comprises dextran.7. The method of claim 1 , wherein the protein hydrolysate comprises a hydrolysate of whey protein.8. The method of claim 1 , wherein the protein hydrolysate comprises a hydrolysate of whey protein isolate.9. The method of claim 1 , wherein the protein hydrolysate comprises a hydrolysate of β-lactoglobulin.10. A polysaccharide-protein conjugate obtained by reacting a polysaccharide comprising a reducing sugar and a protein hydrolysate in an aqueous solution under temperature conditions of from about 40° C. to about 120° C. claim 1 , thereby producing a polysaccharide-protein conjugate.11. The polysaccharide-protein conjugate of claim 10 , wherein the polysaccharide ...

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Номер записи: 28
20-02-2020 дата публикации

Methods and Compositions for Synthesizing Improved Silk Fibers

Номер: US20200055996A1
Автор: Breslauer David N., Kittleson Joshua, Turner Brendan, Widmaier Daniel M., Wray Lindsay
Принадлежит:

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers. 1. An expression construct encoding a proteinaceous block co-polymer , comprising a polypeptide , the polypeptide comprising from 2 to 8 concatenated repeats of SEQ ID NO: 1396 or circularly permuted variants thereof.2. The expression construct of claim 1 , further comprising a secretion signal operatively linked to the coding sequence for the block co-polymer polypeptide.3. The expression construct of claim 2 , wherein the secretion signal comprises an alpha mating factor leader and pro sequence.4. The expression construct of claim 1 , wherein the polypeptide comprises a property selected from the group consisting of an alanine composition from 12 to 40% claim 1 , a glycine composition from 25 to 50% claim 1 , a proline composition from 9 to 20% claim 1 , a β-turn composition from 15 to 37% claim 1 , a GPG amino acid motif content from 18 to 55% claim 1 , and a poly-alanine amino acid motif content from 9 to 35%.5. The expression construct of claim 1 , the polypeptide consisting of 3 concatenated repeats of SEQ ID NO: 1396.6. A host cell claim 1 , comprising: the expression construct of .7. The host cell of claim 6 , wherein the cell is selected from the group consisting of: a yeast cell claim 6 , a fungal cell claim 6 , and a gram positive bacteria cell.8Trichoderma.. The host cell of claim 7 , wherein the cell is from the genus9. A method of producing a proteinaceous block co-polymer comprising: a polypeptide comprising from 2 to 8 concatenated repeats of SEQ ID NO: 1396 or circularly permuted variants thereof claim 7 , the method ...

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Номер записи: 29
09-03-2017 дата публикации

Green technology for crosslinking protein molecules for various uses

Номер: US20170065049A1
Автор: Anil Netravali, Yidong ZHONG
Принадлежит: CORNELL UNIVERSITY

The present disclosure relates to, inter alia, a green technology for crosslinking protein molecules for various uses, where the protein molecules can be contained in protein fibers such as, but not limited to, human hair, animal fibers, and mixtures thereof. In one aspect, the present disclosure relates to a crosslinking agent comprising an oxidized sugar having at least two aldehyde groups. In another aspect, the present disclosure relates to a method of crosslinking protein fibers. This method involves providing the aforementioned crosslinking agent and infiltrating a plurality of non-crosslinked protein fibers with the crosslinking agent under conditions effective to cause protein molecules contained in the non-crosslinked protein fibers to become crosslinked, thereby yielding a population of crosslinked protein fibers.

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Номер записи: 30
27-02-2020 дата публикации

Dendritic Macroporous Hydrogels Prepared By Crystal Templating

Номер: US20200062919A1
Автор: Schmidt Christine, Zawko Scott
Принадлежит:

The present invention includes a hydrogel and a method of making a porous hydrogel by preparing an aqueous mixture of an uncrosslinked polymer and a crystallizable molecule; casting the mixture into a vessel; allowing the cast mixture to dry to form an amorphous hydrogel film; seeding the cast mixture with a seed crystal of the crystallizable molecule; growing the crystallizable molecule into a crystal structure within the uncrosslinked polymer; crosslinking the polymer around the crystal structure under conditions in which the crystal structure within the crosslinked polymer is maintained; and dissolving the crystals within the crosslinked polymer to form the porous hydrogel. 1. A hydrogel that comprises molecular pores, the hydrogel comprising: one or more crosslinked polymers formed about a plurality of pre-formed molecular crystals, wherein the crystals are formed while the polymer is in an uncrosslinked form and the molecular crystals form a porous structure within the polymers during the crosslinking of the polymers. This application is a continuation of U.S. patent application Ser. No. 15/890,719, filed Feb. 7, 2018, which is a continuation of U.S. patent application Ser. No. 15/135,978, filed Apr. 22, 2016, now U.S. Pat. No. 9,896,561, issued Feb. 20, 2018, which is a continuation of U.S. patent application Ser. No. 14/277,170, filed May 14, 2014, now U.S. Pat. No. 9,320,827, issued Apr. 26, 2016, which is a continuation of U.S. patent application Ser. No. 13/909,707, filed Jun. 4, 2013, now U.S. Pat. No. 8,728,499, issued May 20, 2014, which is a continuation of U.S. patent application Ser. No. 12/919,667, filed Aug. 26, 2010, now U.S. Pat. No. 8,668,863, issued Mar. 11, 2014, which claims priority to Patent Cooperation Treaty Application Serial No. PCT/US2009/035257, filed Feb. 26, 2009, which claims priority to U.S. Provisional Patent Application Ser. No. 61/031,651, filed Feb. 26, 2008 and entitled “DENDRITIC MACROPOROUS HYDROGELS PREPARED BY CRYSTAL ...

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Номер записи: 31
11-03-2021 дата публикации

MODIFIED BIOPOLYMERS AND METHODS OF PRODUCING AND USING THE SAM

Номер: US20210070888A1
Автор: Ayoub Ali, Bray James Charles, Chan Ryan Nicholas
Принадлежит:

Modified biopolymers, such as, charge-modified biopolymers, cross-linked biopolymers, and cross-linked, charged modified biopolymers are provided along with methods of producing and using the same. 1. A method for producing a cross-linked , charge-modified biopolymer comprising:combining a biopolymer and a plasticizer to form a homogenous reaction blend, wherein the homogenous reaction blend comprises a plasticized biopolymer;reacting the plasticized biopolymer and at least one charge-modifying agent in the homogenous reaction blend to form a charge-modified biopolymer; andcross-linking the charge-modified biopolymer to form a cross-linked, charge-modified biopolymer,wherein the cross-linked, charge modified starch is free of crystalline domains.2. The method of claim 1 , wherein the combining step further comprises combining a catalyst with the biopolymer and the plasticizer to form the homogenous reaction blend.3. The method of claim 1 , wherein the cross-linking step further comprises reacting the charge-modified biopolymer with at least one cross-linking agent.4. The method of claim 1 , wherein the biopolymer comprises at least two different biopolymers.5. The method of claim 1 , wherein the cross-linked claim 1 , charge-modified biopolymer has a net positive charge.6. The method of claim 1 , wherein the combining step comprises melt blending the biopolymer and the plasticizer using a reactive extrusion process.7. The method of claim 1 , wherein the method is carried out in an extruder.8. The method of claim 1 , wherein the cross-linked claim 1 , charge-modified biopolymer is in the form of a particle have a diameter in a range of about 10 microns to about 1000 microns.9. The method of claim 1 , wherein the reacting and/or cross-linking step(s) is/are carried out at a temperature in a range of about 80° C. to about 150° C.10. The method of claim 1 , further comprising heating the cross-linked claim 1 , charge-modified biopolymer at a temperature in a range of ...

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Номер записи: 32
12-03-2020 дата публикации

Protein-containing adhesives, and manufacture and use thereof

Номер: US20200079983A1
Автор: Anthony A. Parker, Joseph J. Marcinko
Принадлежит: Evertree SAS

The invention provides protein adhesives and methods of making and using such adhesives. The protein adhesives contain ground plant meal or an isolated polypeptide composition obtained from plant biomass.

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Номер записи: 33
21-03-2019 дата публикации

BINDER CONTAINING WHEY PROTEIN

Номер: US20190085204A1
Автор: Hampson Carl, Pacorel Benedicte
Принадлежит:

The present invention relates to an aqueous binder composition, a method of manufacturing a product comprising said binder composition in a cured state, as well as particle or fibre products comprising the binder composition in a cured state, and a use of said binder composition. 1. A method of manufacturing a product selected from a building product , a mineral wool insulation product , a wood product , an automotive product , a paper product and a refractory product , comprising the steps of:(a) providing a collection of matter,(b) applying an aqueous binder composition to the collection of matter, the aqueous binder composition comprising, by dry weight:i) between 65 wt % and 90 wt % whey protein;ii) between 0.5 wt % and 15 wt % carbohydrate;iii) between 0.5 wt % and 15 wt % fat; andiv) between 0.5 wt % and 15 wt % ash; and(c) curing the binder applied to the collection of matter by applying energy to the collection of matter to form the product.2. The method according to claim 1 , wherein the binder holds the collection of matter together to form the product.3. The method according to claim 1 , wherein the collection of matter comprises matter selected from the group consisting of wood claim 1 , wood particles claim 1 , cellulose fibres and mineral fibres.4. The method according to claim 1 , wherein the aqueous binder composition comprises at least 95% by dry weight of whey protein preparation.5. The method according to claim 1 , wherein the aqueous binder composition comprises at least one additive selected from the group consisting of silanes claim 1 , waxes claim 1 , catalysts claim 1 , surfactants and corrosion inhibitors claim 1 , wherein the total amount of silanes claim 1 , waxes claim 1 , catalysts claim 1 , surfactants and corrosion inhibitors by dry weight in the aqueous binder composition is in the range of 0.2 wt % to 6 wt %.6. The method according to claim 1 , wherein the aqueous binder composition further comprises one or more polymerization ...

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Номер записи: 34
30-03-2017 дата публикации

Methods and Compositions for Synthesizing Improved Silk Fibers

Номер: US20170088675A1
Автор: Breslauer David N., Kittleson Joshua, Turner Brendan, Widmaier Daniel M., Wray Lindsay
Принадлежит:

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers. 1. A fiber spinning solution , comprisinga polypeptide, the polypeptide comprising from 2 to 8 concatenated repeats of SEQ ID NO: 1396 or circularly permuted variants thereof, the polypeptide further comprising a FLAG tag; anda spinning solvent, wherein said polypeptide is dissolved in said spinning solvent.2. The fiber spinning solution of claim 1 , wherein said spinning solvent comprises formic acid.3. The fiber spinning solution of claim 1 , wherein said spinning solvent is formic acid.4. The fiber spinning solution of claim 1 , wherein said fiber spinning solution comprises from 20-30% of said polypeptide by weight.5. The fiber spinning solution of claim 1 , wherein the FLAG tag is on the C-terminal end of the polypeptide.6. The fiber spinning solution of claim 1 , wherein said FLAG tag is a 3×FLAG tag.7. The fiber spinning solution of claim 1 , wherein said FLAG tag is encoded by a polynucleotide comprising SEQ ID NO: 1409.8. The fiber spinning solution of claim 1 , wherein the polypeptide comprises a property selected from the group consisting of an alanine composition from 12 to 40% claim 1 , a glycine composition from 25 to 50% claim 1 , a proline composition from 9 to 20% claim 1 , a β-turn composition from 15 to 37% claim 1 , a GPG amino acid motif content from 18 to 55% claim 1 , and a poly-alanine amino acid motif content from 9 to 35%.9. The fiber spinning solution of claim 1 , wherein the polypeptide comprises exactly 3 concatenated repeats of SEQ ID NO: 1396.10. A method of making a silk fiber claim 1 , comprising:{'claim-ref ...

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Номер записи: 35
05-05-2022 дата публикации

Protein hydrogel, preparation method and use thereof

Номер: US20220135749A1
Автор: Marcin Przemyslaw KRZYKAWSKI, Renata KRZYKAWSKA
Принадлежит: Real Research Sp Z OO

The invention relates to a new protein hydrogel created on the basis of low-concentrated components: reagents A and B, the method of hydrogel preparation and its use.

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Номер записи: 36
05-05-2022 дата публикации

BIOFUNCTIONALIZED HYDROGEL FOR CELL CULTURE

Номер: US20220135953A1
Автор: "OGrady Brian", Balotin Kylie, Bellan Leon M., Lippmann Ethan S.
Принадлежит:

Provided are biomaterials useful for cell culture, method of preparation thereof, and use thereof. The present biomaterial comprises a crosslinked hydrogel and a peptide chemically attached to the hydrogel, wherein the peptide comprises a histidine-alanine-valine (HAV) sequence. In particular, the present biomaterial may be useful for culturing neurons, brain endothelial cells, and/or glial cells, supporting the formation of synaptically connected neural networks, and growing stem cell-derived organoids that more closely resemble human organs.

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Номер записи: 37
05-04-2018 дата публикации

Microcarriers, Matrices And Scaffolds For Culturing Mammalian Cells And Methods Of Manufacture

Номер: US20180094080A1
Автор: Mitchell James W., Yang Dazhi
Принадлежит: HOWARD UNIVERSITY

Microcarriers, matrices and scaffolds for growing mammalian cells are provided which include copolymer particles and matrices comprising of polysaccharide-polyamine copolymers. The copolymeric particles and matrices have a pore size of at least 50 microns and permit the mammalian cells to grow both on an exterior surface of the particles and matrices and within an interior of the particles and matrices. Methods for making such microcarriers, matrices and scaffolds, and compositions are also provided. Methods for growing mammalian cells utilizing such microcarriers, matrices and scaffolds and compositions are also provided. 1. A method of producing polysaccharide-polyamine copolymers or glycoprotein-polyamine copolymers , the method comprising the steps of:providing an oxidized polysaccharide or oxidized glycoprotein having aldehyde moieties;reacting the oxidized polysaccharide or oxidized glycoprotein with an amino polymer to form a polymer containing imine derivatives; andconverting the imine derivatives on the polymer to amines to form the polysaccharide-polyamine copolymers or glycoprotein-polyamine copolymers, the polysaccharide-polyamine copolymers or glycoprotein-polyamine copolymers having an amino functionality which will provide a cationic copolymeric material having a three-dimensional structure with cationic sites when protonated.2. The method of wherein the polysaccharide-polyamine copolymers or glycoprotein-polyamine copolymers are di-block copolymers.3. The method of any one of wherein the aldehyde moieties are generated by selectively oxidizing hydroxyl groups on C2 and C3 of glucose units and the oxidation does not produce more carboxyl groups than aldehyde groups or cause cleavage of a polysaccharide chain.4. The method of further comprising the step of drying the polysaccharide-polyamine copolymers or glycoprotein-polyamine copolymers to form polysaccharide-polyamine copolymer particles or glycoprotein-polyamine copolymer particles.5. The method of ...

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Номер записи: 38
28-03-2019 дата публикации

PROTEIN-CONTAINING ADHESIVES, AND MANUFACTURE AND USE THEREOF

Номер: US20190092988A1
Автор: Marcinko Joseph J., Parker Anthony A.
Принадлежит:

The invention provides protein adhesives containing certain additives and methods of making and using such adhesives. The protein adhesives contain ground plant meal or an isolated polypeptide composition obtained from plant biomass in combination with certain additives, such as an exfoliated clay or partially exfoliated clay. 1. An adhesive composition in the form of an emulsion , comprising:(a) from about 5% to about 50% (w/w) of a reactive prepolymer, wherein the reactive prepolymer is an organic polyisocyanate;(b) ground plant meal in an amount sufficient to disperse the reactive prepolymer in an aqueous medium, wherein the ground plant meal is derived from corn, wheat, sunflower, cotton, rapeseed, canola, castor, soy, camelina, flax, jatropha, mallow, peanuts, sugarcane bagasse, tobacco, or a combination thereof; the ground plant meal being present in an amount from about 5% to about 35% (w/w) of the adhesive composition;(c) from about 0.1% to about 5% w/w of at least one first additive selected from the group consisting of a partially exfoliated clay, an exfoliated clay, an intercalated clay, cellulose nanoparticles, and a mixture of a silicone and a terpene compound; and(d) from about 30% (w/w) to about 60% (w/w) water.2. The composition of claim 1 , wherein the ground plant meal has a particle size in the range of from about 1 μm to about 200 μm.37-. (canceled)8. The composition of claim 1 , wherein the ground plant meal is present in an amount from about 10% to about 30% (w/w) of the adhesive composition.9. The composition of claim 1 , wherein the ground plant meal is derived from rapeseed claim 1 , canola claim 1 , castor claim 1 , soy claim 1 , or a combination thereof.10. (canceled)11. (canceled)12. The composition of claim 1 , wherein the first additive is a partially exfoliated clay.13. (canceled)14. The composition of claim 12 , wherein the partially exfoliated clay is a partially exfoliated smectite.15. (canceled)16. The composition of claim 1 , ...

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Номер записи: 39
02-04-2020 дата публикации

Composition for a Molded Body

Номер: US20200102424A1
Автор: BOULET-AUDET Maxime, EL-DIFRAWY Nour Eldien, Guerette Paul Andre, Kittleson Joshua Tyler, RICE Gregory Wilson, Visjager Jeroen, Wray Lindsay
Принадлежит:

The present disclosure relates to a composition for a molded body comprising a recombinant spider silk protein, and a plasticizer. Further, the present disclosure relates to a molded body comprising a recombinant spider silk protein and a plasticizer, and a process for preparing the molded body. 1. A composition for a molded body comprising a recombinant spider silk protein and a plasticizer , wherein the composition is capable of being induced into a flowable state , wherein the recombinant spider silk protein is substantially non-degraded in the flowable state.237.-. (canceled)38. A process for preparing a molded body , comprising the steps of:(a) applying pressure and shear force to a composition comprising a recombinant spider silk protein and a plasticizer to transform the composition to a flowable state, and(b) extruding the composition in the flowable state to form a molded body.39. The process of claim 38 , wherein extruding the composition to form a molded body comprises extruding the composition to form a fiber or extruding the composition into a mold.40. The process of claim 39 , wherein extruding the composition to form a fiber comprises extruding the composition through a spinneret.41. (canceled)42. The process of claim 38 , further comprising:(a) applying pressure and shear force to the molded body to transform the molded body to a composition in a flowable state, and(b) extruding the composition in the flowable state to form a second molded body.43. The process of claim 42 , further comprising repeating steps (a) and (b) to the second molded body at least once.44. The process of claim 38 , wherein said shear force is from 1.5 to 13 Nm.45. (canceled)46. The process of claim 38 , wherein the shear force and pressure are applied to the composition using a capillary rheometer or a twin screw extruder.47. The process of claim 46 , wherein the screw speed of the twin screw extruder ranges from 10 to 300 RPM during application of said pressure and shear ...

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Номер записи: 40
10-07-2014 дата публикации

Silica Compositions

Номер: US20140194341A1
Автор: Barry L. Mcgraw, Phillip DENEN, Ramanathan S. Lalgudi
Принадлежит: Battelle Memorial Institute Inc

Compositions are provided, the compositions comprising: a silica compound containing at least one nitrogen atom; and a carboxylated polymer containing at least one nitrogen atom. The compositions may be useful to control algal growth, including harmful algal blooms (HAB).

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Номер записи: 41
27-04-2017 дата публикации

ARTIFICIAL BIOMEMBRANE USING COCOON AND METHOD FOR MANUFACTURING SAME

Номер: US20170112966A1
Автор: JO You Young, KIM Hyun Bok, KWEON Hae Yong, LEE Heui Sam, LEE Kwang Gill, YEO Joo Hong
Принадлежит: REPUBLIC OF KOREA (MANAGEMENT:RURAL DEVELOPMENT ADMINISTRATION)

Disclosed herein are a cocoon-based artificial biomembrane and a method for manufacturing the same. A cocoon the shell of which has a first thickness is divided into two or more fragments in predetermined form. The cocoon fragments may be used as artificial biomembranes. They can be relatively simply manufactured in a more cost efficient manner than conventional artificial biomembranes and have excellent cell growth potential. Also contemplated are a cocoon-based artificial biomembrane having excellent tensile strength and elongation, and a method for manufacturing the same. 1. A cocoon-based artificial biomembrane , prepared by dividing a cocoon into two or more fragments in a predetermined form , the cocoon having a shell with a first thickness.2. The cocoon-based artificial biomembrane of claim 1 , wherein each of the fragments is delaminated into a lamellar fragment with a second thickness claim 1 , the second thickness being smaller than the first thickness.3. The cocoon-based artificial biomembrane of claim 2 , wherein the lamellar fragment with a second thickness is an inner stratum of the cocoon.4. The cocoon-based artificial biomembrane of claim 2 , wherein the lamellar fragment with a second thickness is a mid stratum of the cocoon.5. The cocoon-based artificial biomembrane of claim 2 , wherein the lamellar fragment with a second thickness is an outer stratum of the cocoon.6. The cocoon-based artificial biomembrane of claim 3 , wherein the lamellar fragment is sterilized.7. The cocoon-based artificial biomembrane of claim 4 , wherein the lamellar fragment is sterilized.8. The cocoon-based artificial biomembrane of claim 5 , wherein the lamellar fragment is sterilized.9. The cocoon-based artificial biomembrane of claim 6 , wherein the lamellar fragment is packed.10. A method for manufacturing a cocoon-based artificial biomembrane claim 6 , comprising a first step of dividing a cocoon into two or more fragments in a predetermined form claim 6 , the cocoon ...

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Номер записи: 42
03-05-2018 дата публикации

Corn protein isolate and methods of manufacturing same

Номер: US20180118780A1
Автор: Craig A. Wilson, Eugene Max Peters, Jr., Guo-Hua Zheng, Michael A. Porter, Yumin Chen
Принадлежит: Cargill Inc

Aspects of the present invention provide a corn protein isolate, comprising at least about 85 wt % corn protein on a dry basis; an “a*” color value ranging from about −0.5 and 1.5, and a “b” color value ranging from about 10 and 25; and less than about 1.5% oil on a dry basis. Further aspects include methods of making the same.

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Номер записи: 43
27-05-2021 дата публикации

DEGRADABLE THIOL-ENE POLYMERS AND METHODS OF MAKING THEREOF

Номер: US20210154306A1
Автор: KAZANTSEV Alexei V., MARINER Peter D., STANTON Martin
Принадлежит:

Provided are methods for linking polypeptides (including peptides and proteins) to other moieties using radical imitated thiol-ene chemistries, for example, modifying a polypeptide by introducing reactive thiol groups and reacting the thiol groups with olefin-containing reagents or alkyne-containing reagents under conditions that support radical thiol-ene or thiol-yne reactions. The reactive thiol groups have greater activity for radical thiol-ene reactions that a cysteine thiol group, including thiol groups that are separated from the peptide backbone by at least two carbon atoms, for example, the thiol group of a homocysteine residue. Also provided are compositions and biomaterials containing the linked polypeptides, for example, peptide and protein conjugates, and thiol-ene based biocompatible hydrogel polymers, and their uses in the medical field. 1. A method for selectively linking a polypeptide in the presence of one or more cysteine residues ,wherein the polypeptide comprises a peptide backbone comprising a homocysteine residue having a reactive thiol group or a 2-amino-5-mercaptopentanoic acid residue having a reactive thiol group,wherein the method comprises reacting the reactive thiol group of the homocysteine residue or the 2-amino-5-mercaptopentanoic acid residue of the polypeptide with an ene compound comprising one or more reactive ene groups under conditions that promote a radical-mediated thiol-ene reaction.2. The method according to claim 1 , wherein the radical-mediated thiol-ene reaction is initiated with a radical initiator.3. (canceled)4. The method according to claim 1 , wherein the polypeptide comprises a homocysteine residue.5. (canceled)6. The method according to claim 2 , wherein the radical initiator is a photoinitiator selected from the group consisting of lithium phenyl-2 claim 2 ,4 claim 2 ,6-trimethylbenzoylphosphinate (LAP) claim 2 , sodium phenyl-2 claim 2 ,4 claim 2 ,6-trimethylbenzoylphosphinate (NAP) claim 2 , 2-hydroxy-1-[4-(2- ...

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Номер записи: 44
08-09-2022 дата публикации

METHOD FOR PRODUCING BONE GELATINE, AND PRODUCED BONE GELATINE

Номер: US20220282123A1
Автор: PÖRSCHKE Ralf
Принадлежит:

The present invention relates to a method for producing bone gelatine having an isoelectric point of less than 6, comprising the following steps: a) providing bones of vertebrates; b) mechanically crushing the bones to a particle size of less than 1 500 μm, preferably less than 500 μm, more preferably less than 300 μm; c) extracting the crushed bones using an aqueous medium at a temperature of from 100 to 140° C., preferably from 120 to 130° C., for a period of from 0.5 to 10 min, preferably 1 to 5 min, more preferably 1 to 3 min; d) separating off the aqueous gelatine solution from the crushed bones; and e) drying the aqueous gelatine solution in order to obtain the bone gelatine having an isoelectric point of less than 6, wherein the method does not comprise liming of the bones with a base, and wherein the bones provided in step a) have not undergone liming. The invention further relates to bone gelatine having an isoelectric point of less than 6, produced by this method. 2. The method according to claim 1 , wherein the method does not comprise maceration of the bones with an acid claim 1 , and wherein the bones provided in step a) have not undergone maceration.3. The method according to claim 1 , wherein the bones come from mammals.4. The method according to claim 1 , wherein the bones are cleaned before being crushed.5. The method according to claim 4 ,wherein the cleaning of the bones comprises a treatment with one or more enzymes.6. The method according to claim 1 , wherein claim 1 , before crushing claim 1 , the bones have a fat content of less than 4 weight %.7. The method according to claim 1 , wherein the mechanical crushing comprises dry grinding or wet grinding of the bones.8. The method according to claim 1 , wherein the extraction is carried out with a content of crushed bones by weight in the aqueous medium of from 0.05 to 0.5 kg/1.9. The method according to claim 1 , wherein the extraction is carried out at a pH value in the range of from 7.5 to 9.10 ...

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Номер записи: 45
31-05-2018 дата публикации

Affinity-assisted protein modification and recycling

Номер: US20180148472A1
Автор: Christian B. Rosen, Matthew B. Francis, Meera Rao, Richard L. Kwant
Принадлежит: UNIVERSITY OF CALIFORNIA

Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.

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Номер записи: 46
09-06-2016 дата публикации

Polymers and plastics derived from animal proteins

Номер: US20160160048A1
Автор: David Curtis BRESSLER, Phillip CHOI
Принадлежит: University of Alberta

The invention is directed to a method for preparing a polymer derived from an animal protein, such as in a feedstock derived from animal by-products. The method involves hydrolyzing proteins present in a feedstock to obtain hydrolyzed proteins, wherein hydrolysis is conducted under conditions sufficient to digest the proteins and destroy pathogens; extracting a protein fraction from the hydrolyzed proteins; and treating the protein fraction with a crosslinking reagent to form the polymer. The crosslinking reagents may include epoxies. The polymer may be further processed to form a thermoset plastic. The polymer may be used as an adhesive, or in the preparation of a natural fiber composite material.

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Номер записи: 47
14-06-2018 дата публикации

Dendritic Macroporous Hydrogels Prepared By Crystal Templating

Номер: US20180163012A1
Автор: Schmidt Christine, Zawko Scott
Принадлежит:

The present invention includes a hydrogel and a method of making a porous hydrogel by preparing an aqueous mixture of an uncrosslinked polymer and a crystallizable molecule; casting the mixture into a vessel; allowing the cast mixture to dry to form an amorphous hydrogel film; seeding the cast mixture with a seed crystal of the crystallizable molecule; growing the crystallizable molecule into a crystal structure within the uncrosslinked polymer; crosslinking the polymer around the crystal structure under conditions in which the crystal structure within the crosslinked polymer is maintained; and dissolving the crystals within the crosslinked polymer to form the porous hydrogel. 1. (canceled)2. An apparatus comprising:a hydrogel film having first and second opposing ends;wherein the hydrogel film includes (a)(i) uncrosslinked hyaluronic acid, (a)(ii) crosslinked alginate, and (a)(iii) at least one of a protein and a biomineral;wherein (b)(i) the alginate is crosslinked with calcium, and (b)(ii) the alginate is crosslinked around the uncrosslinked hyaluronic acid.3. The apparatus of wherein the hydrogel film includes the biomineral.4. The apparatus of wherein the hydrogel film includes the protein.5. The apparatus of wherein the protein includes a cell adhesive protein.6. The apparatus of wherein the hydrogel film includes a drug.7. The apparatus of included in a kit claim 4 , the kit comprising a calcium chelator.8. The apparatus of claim 4 , wherein the hydrogel film consists essentially of the uncrosslinked hyaluronic acid and the crosslinked alginate.9. The apparatus of wherein the hydrogel film includes chitosan.10. The apparatus of wherein the hydrogel film includes urea.11. The apparatus of wherein the hydrogel film includes at least one crystal.121. The apparatus of wherein the at least one crystal is (a) dendritically branched claim 11 , and (b) at least micron in diameter.13. The apparatus of wherein the at least one crystal comprises cyclodextrin.14. An ...

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Номер записи: 48
01-07-2021 дата публикации

BIFUNCTIONAL MODIFIED BIOPOLYMER BASED POLYMERS AND HYDROGELS OBTAINABLE FROM SUCH BIFUNCTIONAL MODIFIED BIOPOLYMER BASED POLYMERS

Номер: US20210198433A1
Автор: Van Hoorick Jasper, Van Vlierberghe Sandra
Принадлежит:

The invention relates to a bifunctional modified biopolymer based polymer, comprising at least one polymer chain comprising n first functional groups and m second functional groups. The first functional groups comprise groups able of being radically cross-linked following a free radical chain- growth polymerisation. The second functional groups comprise groups able to thiol-ene crosslinking. Preferred bifunctional modified biopolymer based polymers comprise bifunctional modified gelatin and bifunctional modified collagen. The invention further relates to a method to prepare such a bifunctional modified biopolymer based polymer and to a method to prepare a hydrogel starting from such bifunctional modified biopolymer based polymer. Furthermore the invention relates to hydrogels obtainable starting from such bifunctional modified biopolymer based polymers and to the use of such hydrogels. 115-. (canceled)16. A bifunctional modified biopolymer based polymer comprising at least one polymer chain , wherein:the at least one polymer chain comprises n first functional groups and m second functional groups, with both n and m not being zero;the first functional groups comprise groups that are radically cross-linkable following a free radical chain-growth polymerization; andthe second functional groups comprise thiol-ene cross-linkable groups that remain unreacted during free radical chain-growth polymerization of the first functional groups.17. The bifunctional modified biopolymer based polymer of claim 16 , wherein the biopolymer based polymer is selected from the group consisting of polypeptides claim 16 , proteins claim 16 , polysaccharides claim 16 , nucleic acids claim 16 , gelatins claim 16 , collagens claim 16 , alginates claim 16 , dextrans claim 16 , agarose claim 16 , glycosaminoglycans claim 16 , chitosans and carrageenans claim 16 , derivates thereof claim 16 , recombinant analogues thereof claim 16 , and synthetic analogues thereof.18. The bifunctional modified ...

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Номер записи: 49
21-06-2018 дата публикации

CYCLIC RGD CELL-BINDING MOTIF AND USES THEREOF

Номер: US20180170977A1
Автор: Hedhammar My
Принадлежит: SPIBER TECHNOLOGIES AB

A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for α5β1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface. 120.-. (canceled)22. A recombinant fusion protein according to claim 21 , wherein Xis G or A.23. A recombinant fusion protein according to claim 21 , wherein Xis G.24. A recombinant fusion protein according to claim 21 , wherein Xis S.25. A recombinant fusion protein according to claim 21 , wherein Xis G or P.26. A recombinant fusion protein according to claim 25 , wherein Xis P.27. A recombinant fusion protein according to claim 21 , wherein Xis G or A.28. A recombinant fusion protein according to claim 27 , wherein Xis A.29. A recombinant fusion protein according to wherein the cell-binding motif is comprising the amino acid sequence CTGRGDSPAC (SEQ ID NO: 10).30. A recombinant fusion protein according to claim 21 , wherein the cell-binding motif has selectivity for α5β1 integrins.31. A recombinant fusion protein according to claim 21 , wherein the spidroin fragment is comprising the protein moiety CT claim 21 , whereinCT is a fragment of from 70 to 120 amino acid residues, having at least 70% identity to any one of SEQ ID NO: 3 and 29-59.32. A recombinant fusion protein according to claim 31 , wherein CT has at least 80% identity to any one of SEQ ID NO: 3 and 29-59.33. A recombinant fusion protein according to claim 31 , wherein CT has at least 80% identity to SEQ ID NO: 3.34. A recombinant fusion protein according to claim 21 , wherein the spidroin fragment is further comprising the protein moiety REP claim 21 , wherein{'sub': n', 'n', 'n', 'n, 'REP is a repetitive fragment of from 70 to 300 amino acid residues, selected from the group consisting of L(AG)L, L(AG)AL, L(GA)L, and L(GA)GL, wherein'}n is an integer from 2 to 10;each individual A segment is an amino acid ...

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Номер записи: 50
15-07-2021 дата публикации

EPOXIDIZED NATURAL RUBBER COMPOSITE AND PREPARATION PROCESS THEREOF

Номер: US20210214532A1
Автор: Liao Lusheng, Liao Shuangquan, PENG Zheng, Wei Yanchan, Yang Changjin
Принадлежит: Hainan University

The present disclosure provides an epoxidized natural rubber composite and a preparation process thereof, and relates to the technical field of rubber materials. The epoxidized natural rubber composite provided by the present disclosure comprises the following preparation raw materials in parts by weight: 100 parts of epoxidized natural rubber, 1˜30 parts of peanut meal, 0.05˜0.8 parts of surfactants, 0.1˜4 parts of coagulant and 0.2˜12 parts of vulcanization processing aids. The present disclosure utilizes essential amino acids and non-essential amino acids contained in peanut meal to improve the aging resistance of the epoxidized natural rubber. After hot air aging, ozone aging and ultraviolet aging treatments, both the tensile strength retention rate and the elongation at break retention rate of the epoxidized natural rubber composites of the present disclosure can be kept above 83%. 1. An epoxidized natural rubber composite , wherein , it comprises the following preparation raw materials in parts by weight:100 parts of epoxidized natural rubber, 1˜30 parts of peanut meal, 0.05˜0.8 parts of surfactants, 0.1˜4 parts of coagulant, and 0.2˜12 parts of vulcanization processing aids.2. The epoxidized natural rubber composite according to claim 1 , wherein claim 1 , the epoxidization degree of the epoxidized natural rubber is 2˜50.3. The epoxidized natural rubber composite according to claim 1 , wherein claim 1 , the particle size of the peanut meal is 0.30˜0.45 mm.4. The epoxidized natural rubber composite according to claim 1 , wherein claim 1 , the surfactants include at least one of sodium dodecyl sulfonate claim 1 , sodium dodecyl sulfate claim 1 , sodium lignin sulfonate and calcium lignin sulfonate.5. The epoxidized natural rubber composite according to claim 1 , wherein claim 1 , the coagulant is selected from the group consisting of methanol and ethanol.6. The epoxidized natural rubber composite according to claim 1 , wherein claim 1 , the vulcanization ...

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Номер записи: 51
15-07-2021 дата публикации

WOOD ADHESIVE COMPOSITIONS COMPRISING PROTEINS AND POLY (GLYCIDYL ETHER), AND USES THEREOF

Номер: US20210214595A1
Автор: Anderson Kevin R., WESCOTT James Michael
Принадлежит: Cargill, Incorporated

There is disclosed: a wood adhesive composition comprising a protein and a poly(glycidyl ether); a method for using the wood adhesive composition to make a composite wood product; and composite wood products made using the wood adhesive composition. 1. A wood adhesive composition comprising , a protein a wetting agent and a poly(glycidyl ether) , wherein the wood adhesive composition is an aqueous wood adhesive composition comprising from 20 percent by weight to 60 percent by weight dry solids , and wherein the pH of the wood adhesive composition is from 8 to 13.2. The wood adhesive composition of claim 1 , wherein a weight ratio of protein to poly(glycidyl ether) is from 9.1:1 to 2.8:1.3. The wood adhesive composition of claim 1 , wherein a weight ratio of protein to poly(glycidyl ether) is from 8:1 to 4:1.4. (canceled)5. The wood adhesive composition of claim 4 , wherein the protein is in the form of a protein flour.67.-. (canceled)8. The wood adhesive composition of claim 5 , wherein the protein flour comprises from 50 to 96 percent dry weight of the curable wood adhesive composition claim 5 , and the poly(glycidyl ether) comprises from 4 to 50 percent dry weight of the curable wood adhesive composition.9. The wood adhesive composition of claim 5 , wherein the protein flour comprises from 80 to 96 percent dry weight of the curable wood adhesive composition claim 5 , and the poly(glycidyl ether) comprises from 4 to 20 percent dry weight of the curable wood adhesive composition.10. The wood adhesive composition of claim 5 , wherein the plant protein is derived from soybeans claim 5 , peas claim 5 , or mixtures thereof.11. The wood adhesive composition of claim 1 , wherein the protein is in the form of a soy flour comprising from 45 to 55 percent by weight protein.12. The wood adhesive composition of claim 11 , wherein the soy flour has a PDI from 20 to 90.13. The wood adhesive composition of claim 11 , wherein the soy flour has a PDI from 70 to 90.1418.-. (canceled ...

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Номер записи: 52
20-06-2019 дата публикации

USE OF 2,4-DIHALO-6-SUBSTITUTED-1,3,5-TRIAZINES AND DERIVATIVE THEREOF AS CONDENSATION, CROSS-LINKING, TANNING, GRAFTING AND CURING AGENTS

Номер: US20190185627A1
Автор: AGOSTINIS Lodovico, BEGHETTO Valentina
Принадлежит: CROSSING SRL

Use of 2,4-dihalo-6-substituted-1,3,5-triazines as condensing, cross-linking, tanning, grafting, curing agents for the production of amides, esters, thioesters, and stabilized collagen and leather, CMC (carboxymethyl cellulose), synthetic and natural polymers. The process enables to obtain non-toxic and totally free of heavy metals products characterized by Tg values between 80° C. and 100° C. 122-. (canceled)24. The process according to claim 23 , wherein the 2 claim 23 ,4 claim 23 ,6-substituted-1 claim 23 ,3 claim 23 ,5-triazine of formula (I) is a 2 claim 23 ,4-dihalo-1 claim 23 ,3 claim 23 ,5-triazine claim 23 , wherein Rand Rare independently selected from the group consisting of Cl claim 23 , Br and F.25. The process according to claim 23 , wherein Ris selected from the group consisting of OCH claim 23 , OCHCH claim 23 , OCH(CH) claim 23 , OCHCHCH claim 23 , and OCHCHCHCH.26. The process according to claim 23 , wherein Ris selected from the group consisting of OCH claim 23 , OCHCH claim 23 , OCHCHCH.27. The process according to claim 23 , wherein Ris selected from the group consisting of N(CH) claim 23 , N(CHCH) claim 23 , N[CH(CH)] claim 23 , N(CHCHCHCH)and N(CH).28. The process according to claim 23 , wherein the reacting step (a) is carried out at a temperature between 0° C. and 70° C. for 15 minutes to 48 hours.29. The process according to claim 23 , wherein the solvent is selected from the group consisting of an aliphatic ether claim 23 , a halogenate claim 23 , an alcohol claim 23 , a ketone claim 23 , an ester claim 23 , an aromatic hydrocarbon claim 23 , an aliphatic hydrocarbon claim 23 , an amide claim 23 , a carbonate claim 23 , DMSO and water.30. The process according to claim 23 , wherein claim 23 , when Rand Rare both Cl claim 23 , Br claim 23 , or F in formula (I) claim 23 , step (a) further comprises the presence of a linear or branched alkyl tertiary amine claim 23 , aryl tertiary amine or heterocyclic tertiary amine.31. The process according ...

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Номер записи: 53
20-06-2019 дата публикации

Patterned Silk Inverse Opal Photonic Crystals with Tunable, Geometrically Defined Structural Color

Номер: US20190187331A1
Автор: OMENETTO Fiorenzo G., Wang Yu
Принадлежит:

The present disclosure relates to patterned photonic crystals. Provided photonic crystals are large scale silk inverse opals with tunable, geometrically defined structural color. Provided photonic crystals exhibit structural color or a photonic band gap (“PBG”). Provided photonic crystals are is found to be highly sensitive to water vapor and UV irradiation. Provided multicolored photonic macro- or micro-patterns are shown by selectively applying water vapor or UV irradiation through a shadow mask. The present disclosure also provides methods for making and using the same. 1. An article of manufacture , comprising:a silk inverse opal that exhibits structural color when it is exposed to incident electromagnetic radiation;the silk inverse opal, comprising nanoscale periodic cavities characterized by their lattice constants,wherein a lattice constant for at least some of the nanoscale periodic cavities is smaller in one dimension of its unit cell following exposure to water vapor or ultra violet radiation; andwherein the exhibited structural color of the silk inverse opal is blue shifted following the exposure.2. The article of manufacture of claim 1 , wherein the nanoscale periodic cavities have a spherical shape.3. The article of manufacture of any of the preceding claims claim 1 , wherein the spherical nanoscale periodic cavities have substantially a same diameter.4. The article of manufacture of any of the preceding claims claim 1 , wherein the silk inverse opal has an average lattice constant in a range of between about 100 nm and about 600 nm.5. The article of manufacture of any of the preceding claims claim 1 , wherein the silk inverse opal has a face-centered cubic structure.6. The article of manufacture of any of the preceding claims claim 1 , wherein silk inverse opal exhibits vertical anisotropic shrinkage in the (111) plane of the face-centered cubic structure.7. The article of manufacture of any of the preceding claims claim 1 , wherein at least one ...

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Номер записи: 54
27-06-2019 дата публикации

Cosmetic Sponge And Method For Providing A Cosmetic Sponge

Номер: US20190194398A1
Автор: CELIA Wayne
Принадлежит: Diversified Global Technologies, LLC

This invention relates to a cosmetic sponge and a method of providing a cosmetic sponge incorporating a non-denatured collagen and other additives for skin care, cleaning, and cosmetic application. 1. A cosmetic sponge comprising a polymer foam , a non-denatured collagen , and at least one additive , and wherein said non-denatured collagen comprises a fat content.2. The cosmetic sponge of wherein said non-denatured collagen is prepared under pH conditions ranging from approximately 13 to approximately 3.3. The cosmetic sponge of wherein said at least one additive is selected from the group consisting of: skin care agents claim 1 , hydrocolloid absorptive agents claim 1 , medicaments claim 1 , proteins claim 1 , enzymes claim 1 , nucleic acids claim 1 , vitamins claim 1 , soaps claim 1 , hemostatic agents claim 1 , antibacterial agents claim 1 , antifungal agents claim 1 , surfactants claim 1 , pH buffers claim 1 , mineral salts claim 1 , trace elements claim 1 , plant extracts claim 1 , animal extracts claim 1 , disinfecting and sterilizing agents claim 1 , and combinations thereof.4. The cosmetic sponge of wherein said sponge further comprises an agent from the group consisting of: a cross-linking agent claim 1 , a catalyst claim 1 , a surfactant claim 1 , and combinations thereof.5. The cosmetic sponge of wherein said polymer is a superabsorbent polymer.6. A cosmetic sponge comprising a polymer foam claim 1 , a non-denatured collagen claim 1 , and at least one additive claim 1 , and wherein said non-denatured collagen comprises a protein content.7. The cosmetic sponge of claim 6 , wherein said sponge is a reaction product of a mixture comprising a predetermined amount of non-denatured collagen claim 6 , a predetermined amount of water claim 6 , a predetermined amount of a prepolymer claim 6 , and at least one additive.8. The cosmetic sponge of wherein said sponge further comprises an agent from the group consisting of: a cross-linking agent claim 6 , a catalyst ...

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Номер записи: 55
19-07-2018 дата публикации

MATRIX CONSTRUCTION

Номер: US20180200411A1
Автор: Bills Jess, Cox Derek, Tumey David
Принадлежит:

For making a matrix useable in a wound, matrix construction methods are provided, in which a slurry is formulated and then lyophilized. Usage of collagen and chondroitin sulfate (C6S) in the slurry is favored. 137-. (canceled)38. A method of making a matrix useable in a wound , comprising the steps of:a) containing a slurry in a matrix carrier, which is not a tray, wherein the matrix carrier comprises a wound-shaped cavity having a size and shape duplicative of a wound;b) performing a lyophilization step on the slurry while contained in the matrix carrier.39. The method of claim 38 , wherein in the containing step claim 38 , the slurry comprises collagen and chondroitin sulfate.40. The method of claim 39 , wherein in the containing step claim 39 , the slurry comprises collagen claim 39 , chondroitin sulfate and HA.41. The method of claim 40 , wherein in the containing step claim 40 , the slurry comprises collagen claim 40 , chondroitin sulfate claim 40 , HA and fibronectin.42. The method of claim 38 , wherein in the containing step claim 38 , the slurry comprises acetic acid.431. The method of claim claim 38 , wherein the slurry-formulating step proceeds for a time in a range of about 30-120 minutes claim 38 , at a temperature in a range of about 0° C. to 10° C.441. The method of claim claim 38 , wherein the lyophilizing step proceeds for a time in a range of about 24-72 hours claim 38 , at a temperature in a range of about 0° C. to −80° C.45. The method of claim 38 , wherein the slurry-containing step proceeds for a time in a range of about 30-120 minutes claim 38 , at a temperature in a range of about 0° C. to −80° C.46. The method of claim 38 , wherein the lyophilizing step proceeds for a time in a range of about 24-72 hours claim 38 , at a temperature in a range of about 0° C. to −80° C.471. The method of claim claim 38 , wherein in the slurry-formulating step claim 38 , a ratio of collagen to C6S is in a range of about 0.5% to 0.01%.48. The method of claim 38 , ...

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Номер записи: 56
20-07-2017 дата публикации

Preparation and/or Formulation of Proteins Cross-Linked with Polysaccharides

Номер: US20170203001A1
Автор: Jens Sommer-Knudsen
Принадлежит: Elastagen Pty Ltd

Therapeutic compositions and/or formulations are provided, comprising: at least one cross-linked protein matrix, wherein the at least one cross-linked protein matrix comprises at least one protein residue and at least one saccharide-containing residue, and methods of producing the same. The cross-linked protein matrix may be derived from cross-linking a full length or substantially full length protein, such as tropoelastin, elastin, albumin, collagen, collagen monomers, immunoglobulins, insulin, and/or derivatives or combinations thereof, with a saccharide containing cross-linking agent, such as a polysaccharide cross-linking agent derived from, for example, hyaluronic acid or a cellulose derivative. The therapeutic compositions may be administered topically or by injection. The present disclosure also provides methods, systems, and/or kits for the preparation and/or formulation of the compositions disclosed herein.

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Номер записи: 57
20-07-2017 дата публикации

High density fibrous polymers suitable for implant

Номер: US20170203007A1
Автор: Timothy A. Ringeisen, William Christopher Wattengel
Принадлежит: DSM IP ASSETS BV, Kensey Nash BVF Technology LLC

This invention includes malleable, biodegradable, fibrous compositions for application to a tissue site in order to promote or facilitate new tissue growth. One aspect of this invention is a fibrous component that provides unique mechanical and physical properties. The invention may be created by providing a vessel containing a slurry, said slurry comprising a plurality of natural or synthetic polymer fibers and at least one suspension fluid, wherein the polymer fibers are substantially evenly dispersed and randomly oriented throughout the volume of the suspension fluid; applying a force, e.g., centrifugal, to said vessel containing said slurry, whereupon said force serves to cause said polymer fibers to migrate through the suspension fluid and amass at a furthest extent of the vessel, forming a polymer material, with said polymer material comprising polymer fibers of sufficient length and sufficiently viscous, interlaced, or interlocked to retard dissociation of said polymer fibers.

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Номер записи: 58
19-07-2018 дата публикации

CATIONIC PEPTIDOPOLYSACCHARIDES WITH EXCELLENT BROAD- SPECTRUM ANTIMICROBIAL ACTIVITIES AND HIGH SELECTIVITY

Номер: US20180201652A1
Автор: Chan Bee Eng Mary, Li Peng
Принадлежит: NANYANG TECHNOLOGICAL UNIVERSITY

The disclosure provides cationic peptidopolysaccharides of Formula I: which has a bacterial peptidoglycan-mimetic structure, and shows outstanding broad spectrum activities against clinically significant bacteria and fungi. The structural affinity of these compounds with microbial cell wall constituents promotes its passage to the cytoplasmic membrane resulting in excellent antimicrobial activity and record high selectivity. The disclosure also describes methods of making and using cationic peptidopolysaccharides of Formula I. 2. The method of claim 1 , wherein in the compound of Formula I:{'sub': 1', '6', '2', '6', '2', '6', '3', '8', '6', '10', '6', '10', '1', '6', '3', '10', '3', '10', '1', '6', '2', '2', '2', '2', '2', '2', '2', '2', '2', 'R', '2', '2', '2', '2', '2', '2', 'k', '3', '2', 'k', '3', '2', 'k', '2', 'k, 'sup': 1', '1', '1', '1', '2', '3', '2', '3', '2', '3', '4', '2', '3', '4', '1', '4', '1', '4', '2', '3', '4', '2', '3', '4', '2', '3', '4', '2', '3', '4', '2', '3, 'R is independently selected from substituted or unsubstituted (C-C)alkyl, substituted or unsubstituted (C-C)alkenyl, substituted or unsubstituted (C-C)alkynyl, substituted or unsubstituted (C-C)cycloalkyl, substituted or unsubstituted (C-C)aryl, substituted or unsubstituted (C-C)aryl(C-C)alkyl, substituted or unsubstituted (C-C)heteroaryl, substituted or unsubstituted (C-C)heteroaryl(C-C)alkyl, (CH)OR, (CH)C(O)R, (CH)C(O)OR, (CH)OC(O)R, (CH)NRR, (CH)C(O)NRR, (CH)OC(O)NRR, (CH)C(NR)NRR, (CH)NRC(O), (CH)NRC(O)OR, (CH)NRC(O)NRR, (CH)NRC(O)NRR, (CH)NRC(NH)NRR, (CH)SH, (CH)S(O)CH, (CH)NRS(O)CH, (CH)S(O)NRRand (CH)NRS(O)NRR; and'}{'sup': 1', '2', '3', '4, 'sub': 1', '6', '2', '6', '2', '6', '3', '8', '6', '10', '6', '10', '1', '6', '3', '10', '3', '10', '1', '6, 'R, R, Rand Rare each independently selected from hydrogen, substituted or unsubstituted (C-C)alkyl, substituted or unsubstituted (C-C)alkenyl, substituted or unsubstituted (C-C)alkynyl, substituted or unsubstituted (C-C)cycloalkyl, ...

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Номер записи: 59
26-07-2018 дата публикации

BINDERS AND MATERIALS MADE THEREWITH

Номер: US20180208505A1
Автор: KISSELL Ronald E., SWIFT Brian Lee, XU Ruijian
Принадлежит:

Binders to produce or promote cohesion in non-assembled or loosely assembled matter. 1160.-. (canceled)161. A thermal or acoustical fiberglass insulation material comprising:(a) a collection of mineral fibers; and{'sup': 3', '3, '(b) a binder disposed on the collection of mineral fibers, wherein the binder consists essentially of i) at least one reaction product of a reducing sugar reactant and an amine reactant, ii) a silicon-containing coupling agent, and iii) optionally, a corrosion inhibitor, wherein the fiberglass material comprises less than about 99% by weight and more than about 75% by weight mineral fibers, and wherein the fiberglass material has a density of from about 0.4 lbs/ftto about 6 lbs/ft.'}162. The fiberglass material of claim 161 , wherein the amine reactant is an ammonium salt of a polycarboxylic acid.163. The fiberglass material of claim 161 , wherein the amine reactant is an ammonium salt of a monomeric polycarboxylic acid.164. The fiberglass material of claim 161 , wherein the density is from about 0.75 lbs/ftto about 2.5 lbs/ft.165. The fiberglass material of claim 161 , wherein the density is from about 2.25 lbs/ftto about 4.25 lbs/ft.166. The fiberglass material of claim 161 , wherein the density is from about 0.4 lbs/ftto about 1.5 lbs/ft.167. The fiberglass material of claim 161 , wherein the density is from about 3.75 lbs/ftto about 5.2 lbs/ft.168. A thermal or acoustical fiberglass insulation material comprising:(a) a collection of mineral fibers; and{'sup': 3', '3, '(b) a binder disposed on the collection of mineral fibers, the binder consisting essentially of i) a mixture of Maillard reactants, ii) a silicon-containing coupling agent, and iii) optionally, a corrosion inhibitor, wherein the fiberglass material comprises less than about 99% by weight and more than about 75% by weight mineral fibers, and wherein the fiberglass material has a density in the range from about 0.4 lbs/ftto about 6 lbs/ft.'}169. A thermal or acoustical ...

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Номер записи: 60
25-06-2020 дата публикации

ENGINEERED LEATHER AND METHODS OF MANUFACTURE THEREOF

Номер: US20200199695A1
Автор: FORGACS Gabor, JAKAB Karoly Robert, MARGA Francoise Suzanne
Принадлежит:

Engineered animal skin, hide, and leather comprising a plurality of layers of collagen formed by cultured animal collagen-producing (e.g., skin) cells. Layers may be formed by elongate multicellular bodies comprising a plurality of cultured animal cells that are adhered and/or cohered to one another; wherein the elongate multicellular bodies are arranged to form a substantially planar layer for use in formation of engineered animal skin, hide, and leather. Further described herein are methods of forming engineered animal skin, hide, and leather utilizing said layers of animal collagen-producing cells. 1. A method of producing an engineered leather , the method comprising:culturing one or more types of collagen-producing cells in vitro;forming a plurality of sheets of extracellular matrix including collagen produced by the one or more types of collagen-producing cells;layering the plurality of sheets to form a body having a volume;allowing the layered sheets to fuse; andprocessing the body by tanning to modify the collagen.2. The method of claim 1 , further comprising preparing a plurality of elongate or spherical multicellular bodies comprising said one or more types of collagen-producing cells claim 1 , wherein the collagen-producing cells are cohered to one another.3. The method of claim 1 , wherein forming the plurality of sheets comprises forming a plurality of planar layers comprising adjacently arranging a plurality of elongate multicellular bodies claim 1 , wherein said elongate multicellular bodies are fused to form a planar layer.4. The method of claim 3 , wherein forming comprises automated deposition of multicellular bodies into said layers without a structural scaffold.5. The method of claim 1 , wherein arranging comprises culturing cells on a support to form a substantially planar layer of collagen above and between the cells.6. The method of claim 1 , wherein allowing the layers to fuse takes place over about 2 hours to about 24 hours.7. The method of ...

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Номер записи: 61
05-08-2021 дата публикации

Dendritic Macroporous Hydrogels Prepared By Crystal Templating

Номер: US20210238379A1
Автор: Schmidt Christine, Zawko Scott
Принадлежит:

The present invention includes a hydrogel and a method of making a porous hydrogel by preparing an aqueous mixture of an uncrosslinked polymer and a crystallizable molecule; casting the mixture into a vessel; allowing the cast mixture to dry to form an amorphous hydrogel film; seeding the cast mixture with a seed crystal of the crystallizable molecule; growing the crystallizable molecule into a crystal structure within the uncrosslinked polymer; crosslinking the polymer around the crystal structure under conditions in which the crystal structure within the crosslinked polymer is maintained; and dissolving the crystals within the crosslinked polymer to form the porous hydrogel. 1. A hydrogel that comprises molecular pores, the hydrogel comprising: one or more crosslinked polymers formed about a plurality of pre-formed molecular crystals, wherein the crystals are formed while the polymer is in an uncrosslinked form and the molecular crystals form a porous structure within the polymers during the crosslinking of the polymers. This application is a continuation of U.S. patent application Ser. No. 16/597,106, filed Oct. 9, 2019, which is a continuation of U.S. patent application Ser. No. 15/890,719, filed Feb. 7, 2018, now U.S. Pat. No. 10,442,911, issued Oct. 15, 2019, which is a continuation of U.S. patent application Ser. No. 15/135,978, filed Apr. 22, 2016, now U.S. Pat. No. 9,896,561, issued Feb. 20, 2018, which is a continuation of U.S. patent application Ser. No. 14/277,170, filed May 14, 2014, now U.S. Pat. No. 9,320,827, issued Apr. 26, 2016, which is a continuation of U.S. patent application Ser. No. 13/909,707, filed Jun. 4, 2013, now U.S. Pat. No. 8,728,499, issued May 20, 2014, which is a continuation of U.S. patent application Ser. No. 12/919,667, filed Aug. 26, 2010, now U.S. Pat. No. 8,668,863, issued Mar. 11, 2014, which claims priority to Patent Cooperation Treaty Application Serial No. PCT/US2009/035257, filed Feb. 26, 2009, which claims priority to U. ...

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Номер записи: 62
04-08-2016 дата публикации

Method and Compositions for Synthesizing Improved Silk Fibers

Номер: US20160222174A1
Автор: Breslauer David N., Kittleson Joshua, Turner Brendan, Widmaier Daniel M., Wray Lindsay
Принадлежит:

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers. 1. A proteinaceous block co-polymer , comprising:a polypeptide, the polypeptide comprising from 2 to 8 concatenated repeats of SEQ ID NO: 1396 or circularly permuted variants thereof.2. The proteinaceous block co-polymer of claim 1 , wherein the polypeptide comprises a property selected from the group consisting of an alanine composition from 12 to 40% claim 1 , a glycine composition from 25 to 50% claim 1 , a proline composition from 9 to 20% claim 1 , a β-turn composition from 15 to 37% claim 1 , a GPG amino acid motif content from 18 to 55% claim 1 , and a poly-alanine amino acid motif content from 9 to 35%.3. The proteinaceous block co-polymer of claim 1 , consisting of: 3 concatenated repeats of SEQ ID NO: 1396.4. A fiber comprising the block co-polymer of .5. The fiber of claim 4 , comprising a circular cross-section and a diameter from 4.48 to 12.7 μm.6. The fiber of claim 4 , wherein the fiber has a yield stress from 24 to 172 MPa.7. The fiber of claim 4 , wherein the fiber has a maximum stress from 54 to 310 MPa.8. The fiber of claim 4 , wherein the fiber has a breaking strain from 2 to 200%.9. The fiber of claim 4 , wherein the fiber has an initial modulus from 1617 to 5820 MPa.10. The fiber of claim 4 , wherein the fiber has a toughness value from 0.5 MJ/mto 59.2 MJ/m.11. An expression construct encoding the proteinaceous block co-polymer of .12. The expression construct of claim 11 , further comprising a secretion signal operatively linked to the coding sequence for the block copolymer polypeptide.13. The expression construct of ...

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Номер записи: 63
11-07-2019 дата публикации

Compositions and methods for selectively sequestering metal ions

Номер: US20190210005A1
Автор: Christopher E.R. Richardson, Elizabeth M. Nolan, Lisa S. Cunden, Matthew D. Shoulders, Stephen J. Lippard
Принадлежит: Massachusetts Institute of Technology

Compositions and methods for the selective sequestration of metal ions are generally described.

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Номер записи: 64
02-07-2020 дата публикации

RESIDUAL SOY FLOUR SUGARS AS CROSSLINKERS FOR ENHANCING MECHANICAL PERFORMANCE OF PROTEIN FIBERS

Номер: US20200207924A1
Автор: Netravali Anil N., PATIL Namrata V.
Принадлежит: CORNELL UNIVERSITY

Disclosed is a method of crosslinking protein fibers, including wool fibers, by (i) providing a crosslinking agent including an oxidized sugar mixture having a plurality of different oxidized sugars of different molecular lengths and having at least two aldehyde groups (e.g., oxidized soy flour sugars); and (ii) infiltrating a plurality of non-crosslinked protein fibers with the crosslinking agent under conditions effective to cause protein molecules contained in the non-crosslinked protein fibers to become crosslinked. This method yields a population of crosslinked protein fibers, where the protein molecules of the non-crosslinked protein fibers include amine groups that react with the aldehyde groups of the oxidized sugars to achieve the crosslinking of the protein molecules to yield the crosslinked protein fibers. 1. A method of crosslinking protein fibers , said method comprising:providing a crosslinking agent comprising an oxidized sugar mixture comprising a plurality of different oxidized sugars of different molecular lengths and having at least two aldehyde groups; andinfiltrating a plurality of non-crosslinked protein fibers with the crosslinking agent under conditions effective to cause protein molecules contained in the non-crosslinked protein fibers to become crosslinked, thereby yielding a population of crosslinked protein fibers,wherein the protein molecules of the non-crosslinked protein fibers comprise amine groups that react with the aldehyde groups of the oxidized sugars to achieve the crosslinking of the protein molecules to yield the crosslinked protein fibers.2. The method according to claim 1 , wherein said infiltrating step is carried out at a temperature and for a length of time sufficient to yield crosslinked protein fibers having improved tensile properties selected from the group consisting of increased tensile strength and increased Young's modulus compared to the non-crosslinked protein fibers.3. The method according to claim 2 , wherein ...

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Номер записи: 65
11-07-2019 дата публикации

Hydrolyzed collagen compositions and methods of making thereof

Номер: US20190211078A1
Автор: Ali ELNAJJAR, Ali MOURAD, Christopher Lippelt, Mark Ernst Brandt
Принадлежит: Avicenna Nutraceutical LLC

Disclosed herein are hydrolyzed collagen compositions. The compositions are inexpensive to make and can be produced without the use of proteolytic enzymes, decolorizing agents, antibacterial and antifungal agents, and the like. Further, the compositions are substantially free of odors and are white to light yellow in color and are suitable to be used as dietary supplements. Also disclosed are methods for producing the compositions.

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Номер записи: 66
23-10-2014 дата публикации

MATRICES COMPRISING A MODIFIED POLYSACCHARIDE

Номер: US20140314722A1
Автор: FORGET Aurelien, Shastri V. Prasad
Принадлежит: ALBERT-LUDWIGS UNIVERSITÄT FREIBURG

The present invention discloses a matrix comprising a modified polysaccharide consisting of repeating disaccharide units whereby in at least 11% of the disaccharide units one primary alcohol group is oxidized into a carboxylic acid group. 1. A matrix comprising a modified polysaccharide consisting of repeating disaccharide units , whereby in at least 11% of the disaccharide units one primary alcohol group is oxidized to a carboxylic acid group.2. The matrix according to claim 1 , wherein in 20-99% of the disaccharide repeat units claim 1 , the primary alcohol group is oxidized to the carboxylic acid group.3. The matrix according to claim 2 , wherein in 50-95% of the repeat disaccharide units claim 2 , the primary alcohol group is oxidized to the carboxylic acid group.4. The matrix according to claim 1 , wherein the modified polysaccharide is blended with an unmodified polysaccharide.5. The matrix according to claim 4 , wherein the modified polysaccharide and/or the not modified polysaccharide is agarose.6. The matrix according to claim 1 , wherein the polysaccharide is of natural origin.7. The matrix according to claim 4 , wherein either the modified polysaccharide or the non-modified polysaccharide is selected from the group consisting of a member of the carrageenan family claim 4 , hyaluronic acid claim 4 , heparin sulfate claim 4 , dermatan sulfate claim 4 , chondroitin sulfate claim 4 , alginate claim 4 , chitosan claim 4 , pullulan and agarose.8. The matrix according to claim 1 , wherein the carboxylic group derived from the oxidation of the primary alcohol group is covalently coupled with a peptide sequence.9. The matrix according to claim 8 , wherein the peptide sequence is selected from the group consisting of the cell adhesion sequence arginin-glycin-aspartic acid claim 8 , the peptide sequences IKVAV and YIGSR or a protein selected from collagen claim 8 , collagen fragments claim 8 , fibronectin and mixtures thereof.10. The matrix according to claim 1 , ...

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Номер записи: 67
17-08-2017 дата публикации

Method for making a biofabricated material containing collagen fibrils

Номер: US20170233834A1
Автор: Amy Congdon, Brendan Patrick Purcell, Darryl Miles Cassingham, David Thomas Williamson, Francoise Suzanne Marga, Stephen M. Spinella, Susan J. Schofer
Принадлежит: Modern Meadow Inc

Described herein is a method for producing a biofabricated material from collagen or collagen-like proteins which are recombinantly produced and which contain substantially no 3-hydroxyproline. The collagen or collagen-like proteins are isolated from animal sources, or produced by recombinant DNA techniques or by chemical synthesis. The collagen or collagen-like proteins are fibrillated, crosslinked, dehydrated and lubricated thus forming the biofabricated material having a substantially uniform network of collagen fibrils.

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Номер записи: 68
17-08-2017 дата публикации

Composite biofabricated material

Номер: US20170233943A1
Автор: Amy Congdon, Brendan Patrick Purcell, David Thomas Williamson, Suzanne Lee
Принадлежит: Modern Meadow Inc

The invention is directed to a composite material comprising a biofabricated material and a secondary component. The secondary component may be a porous material, such as a sheet of paper, cellulose, or fabric that has been coated or otherwise contacted with the biofabricated material. The biofabricated material comprises a uniform network of crosslinked collagen fibrilsand provides strength, elasticity and an aesthetic appearance to the composite material.

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Номер записи: 69
26-08-2021 дата публикации

IMMUNOGENIC COMPOSITIONS COMPRISING CONJUGATED CAPSULAR SACCHARIDE ANTIGENS, KITS COMPRISING THE SAME AND USES THEREOF

Номер: US20210260177A1
Автор: Isturiz Raul Enrique, Jodar Martin-Montalvo Luis Pascual, Reinert Ralf Rene, Watson Wendy Jo
Принадлежит: PFIZER INC.

The present invention relates to new immunogenic compositions comprising conjugated capsular saccharide antigens (glycoconjugates), kits comprising said immunogenic compositions and uses thereof. Immunogenic compositions of the present invention will typically comprise at least one glycoconjugate from a serotype not found in PREVNAR®, SYNFLORIX® and/or PREVNAR 13®. The invention also relates to vaccination of human subjects, in particular infants and elderly, against pneumoccocal infections using said novel immunogenic compositions. 1Streptococcus pneumoniaeS. pneumoniaeS. pneumoniaeS. pneumoniaeS. pneumoniaeS. pneumoniaeS. pneumoniaeS. pneumoniae. A kit comprising: (a) a first immunogenic composition comprising at least one glycoconjugate selected from the group consisting of a glycoconjugate from serotype 15B , a glycoconjugate from serotype 22F , a glycoconjugate from serotype 33F , a glycoconjugate from serotype 12F , a glycoconjugate from serotype 10A , a glycoconjugate from serotype 11A and a glycoconjugate from serotype 8 , wherein said composition is a 1 , 2 , 3 , 4 , 5 , 6 or 7-valent pneumococcal conjugate composition; and (b) a second immunogenic composition comprising at least one glycoconjugate from an serotype selected from the group consisting of serotypes 1 , 3 , 4 , 5 , 6A , 6B , 7F , 9V , 14 , 18C , 19A , 19F , and 23F for simultaneous , concurrent , concomitant or sequential administration of the first and second immunogenic compositions.2S. pneumoniaeS. pneumoniaeS. pneumoniaeS. pneumoniaeS. pneumoniaeS. pneumoniaeS. pneumoniae. The kit of claim 1 , wherein said first immunogenic composition comprises a glycoconjugate from serotype 15B claim 1 , a glycoconjugate from serotype 22F claim 1 , a glycoconjugate from serotype 33F claim 1 , a glycoconjugate from serotype 12F claim 1 , a glycoconjugate from serotype 10A claim 1 , a glycoconjugate from serotype 11A and a glycoconjugate from serotype 8 claim 1 , and wherein said composition is a 7-valent ...

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Номер записи: 70
23-08-2018 дата публикации

Cosmetic Sponge and Method for Providing a Cosmetic Sponge

Номер: US20180237592A1
Автор: CELIA Wayne
Принадлежит: Diversified Global Technologies, LLC

This invention relates to a cosmetic sponge and a method of providing a cosmetic sponge incorporating a non-denatured collagen and other additives for skin care, cleaning, and cosmetic application. 1. A cosmetic sponge comprising a polymer foam , a non-denatured collagen , and at least one additive.2. The cosmetic sponge of wherein the non-denatured collagen is prepared under pH conditions ranging from approximately 13 to approximately 3.3. The cosmetic sponge or wherein the non-denatured collagen is prepared under pH conditions ranging from approximately 10 to approximately 4.4. The cosmetic sponge of wherein the non-denatured collagen is prepared under pH conditions ranging from approximately 9 to approximately 5.5. The cosmetic sponge of wherein the at least one additive is selected from the group consisting of skin care agents claim 1 , hydrocolloid absorptive agents claim 1 , medicaments claim 1 , proteins claim 1 , enzymes claim 1 , nucleic acids claim 1 , vitamins claim 1 , soaps claim 1 , hemostatic agents claim 1 , antibacterial agents claim 1 , antifungal agents claim 1 , surfactants claim 1 , pH buffers claim 1 , mineral salts claim 1 , trace elements claim 1 , plant extracts claim 1 , animal extracts claim 1 , disinfecting and sterilizing agents claim 1 , and combinations thereof.6. The cosmetic sponge of claim 1 , wherein the sponge is a reaction product of a mixture comprising:a predetermined amount of non-denatured collagen; a predetermined amount of a prepolymer; and at least one additive.7. The cosmetic sponge of wherein the mixture further comprises an agent from the group consisting of: a cross-linking agent claim 1 , a catalyst claim 1 , a surfactant claim 1 , and combinations thereof.8. A method for providing a cosmetic sponge comprising the steps of: forming a mixture comprising non-denatured collagen and water; mixing a prepolymer with said mixture to form a foam layer.9. The method of claim 8 , wherein the non-denatured collagen is prepared ...

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Номер записи: 71
30-08-2018 дата публикации

METHOD AND DEVICE FOR PRODUCING A CONVERSION PRODUCT, IN PARTICULAR STARCH PASTE

Номер: US20180244961A1
Автор: Abendschön Günter, NOLL Bernhard
Принадлежит: AHK SERVICE & SOLUTIONS GMBH

A method for continuously producing a conversion product, in particular starch glue, fried starch, dissolved gelatin or protein glue, wherein a starch-containing and/or protein-containing, preferably powdery starting material, in particular flour, starch powder, cereal grains, coarse cereal meal, gelatin powder or gluten powder, is fed to a mixing chamber () and the starting material, preferably powder, descending in the mixing chamber () is subjected to a liquid heated to a processing temperature (T) of at least 50° C. for converting the starting material into the conversion product, in particular to at least a gelatinization temperature of the starch-containing starting material, a protein-dissolving and/or denaturing temperature of the protein-containing starting material or a frying temperature, in the form of a pressure jet () and is thereby conveyed against a baffle () preferably formed by an inner wall of the mixing chamber or by an installation in the mixing chamber. 122710. +A method for continuously producing a conversion product , in particular starch glue , fried starch , dissolved gelatin or protein glue , wherein a starch-containing and/or protein-containing , preferably powdery starting material , in particular flour , starch powder , cereal grains , coarse cereal meal , gelatin powder or gluten powder , is fed to a mixing chamber () and the starting material , preferably powder , descending in the mixing chamber () is subjected to a liquid heated to a processing temperature (T) of at least 50° C. for converting the starting material into the conversion product , in particular to at least a gelatinization temperature of the starch-containing starting material , a protein-dissolving and/or denaturing temperature of the protein-containing starting material or a frying temperature , in the form of a pressure jet () and is thereby conveyed against a baffle () preferably formed by an inner wall of the mixing chamber or by an installation in the mixing ...

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Номер записи: 72
31-08-2017 дата публикации

Novel biofabrication techniques for the implementation of intrinsic tissue geometries to an in vitro collagen hydrogel

Номер: US20170247430A1
Автор: Michael John Yost, Veronica Rodriguez-Rivera
Принадлежит: MUSC Foundation for Research Development, UNIVERSITY OF SOUTH CAROLINA

Methods for reaction electrospinning are provided to form collagen fibers. The method can include: acidifying a collagen in an acidic solvent to form an acidic collagen solution; electrospinning the acidic collagen solution within an alkaline atmosphere (e.g., including ammonia vapor) to form collagen fibers; and collecting the collagen fibers within a salt bath (e.g., including ammonium sulfate). The acidic solvent can include water and an alcohol, and can have a pH of about 2 to about 4 (e.g., including a strong acid, such as HCl). An albumin rubber is also provided, which can include albumin crosslinked with glutaraldehyde.

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Номер записи: 73
23-09-2021 дата публикации

GREEN TECHNOLOGY FOR CROSSLINKING PROTEIN MOLECULES FOR VARIOUS USES

Номер: US20210289906A1
Автор: Netravali Anil N., ZHONG Yidong
Принадлежит: CORNELL UNIVERSITY

The present disclosure relates to, inter alia, a green technology for crosslinking protein molecules for various uses, where the protein molecules can be contained in protein fibers such as, but not limited to, human hair, animal fibers, and mixtures thereof. In one aspect, the present disclosure relates to a crosslinking agent comprising an oxidized sugar having at least two aldehyde groups. In another aspect, the present disclosure relates to a method of crosslinking protein fibers. This method involves providing the aforementioned crosslinking agent and infiltrating a plurality of non-crosslinked protein fibers with the crosslinking agent under conditions effective to cause protein molecules contained in the non-crosslinked protein fibers to become crosslinked, thereby yielding a population of crosslinked protein fibers. 1. A formulation for crosslinking keratin-containing fibers of hair comprising:a crosslinking agent comprising an oxidized sugar having at least two aldehyde groups, wherein the sugar is selected from the group consisting of monosaccharides, disaccharides, trisaccharides, and tetrasaccharides,wherein the keratin-containing fibers of hair comprise a plurality of protein molecules comprising amine groups, andwherein the crosslinking agent is formulated so that the aldehyde groups of the oxidized sugar are effective to react with the amine groups of the protein molecules contained in the keratin-containing fibers to yield a population of crosslinked keratin-containing fibers.2. The formulation of claim 1 , further comprising one or more of a preservative claim 1 , a stabilizer claim 1 , a filler claim 1 , a coloring agent claim 1 , a scent agent claim 1 , a cosmetically acceptable carrier claim 1 , an emulsifier claim 1 , or mixtures thereof.3. The formulation of claim 1 , wherein the formulation is in a form of a paste.4. The formulation of claim 1 , wherein the formulation is in a form of an aqueous solution.5. The formulation of claim 4 , wherein ...

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Номер записи: 74
30-07-2020 дата публикации

BISON FIBER INSULATION AND METHOD OF PRODUCING BISON FIBER INSULATION

Номер: US20200239643A1
Автор: Linton Brian Thomas
Принадлежит:

The disclosed embodiments include a blend of bison fibers, another fiber, and adhesives. The bison fibers are sheared from a bison, scoured, dehaired, blended with various other fibers and/or compositions, carded, and manufactured into insulation. The bison hairs can be categorized by diameter into one of four categories: prime, drop A, drop B, or drop C. Furthermore, bison fiber can be categorized based on length, coarseness, weight, and/or where on the bison it was sheared from. The insulation can be batted, woven, knit, loose, and/or other similar types. The insulation can be used for garments, outdoor equipment, bedding products, and/or other products. The weight of the insulation can be between 40 grams per square meter and 500 grams per square meter. The bison fiber can be blended with recycled polyester, bison fiber, wool, bast fiber, cellulose fiber, and/or synthetic fiber. The adhesives can be low-melt poly, or resin. 1. An insulation comprising: a first batch of bison fiber, wherein the first batch of bison fiber is categorized as any of prime, or drop A;', 'a first fiber blended with the first batch of bison fiber; and', 'a first binding agent operable to bind the first batch of bison fiber and the first fiber; and, 'a lightweight insulation including a second batch of bison fiber, wherein the second batch of bison fiber is categorized as any of drop B, or drop C;', 'a second fiber blended with the second batch of bison fiber; and', 'a second binding agent operable to bind the second batch of bison fiber and the second fiber., 'a heavy weight insulation including2. The method of claim 1 , wherein the first fiber and second fiber is any of recycled polyester claim 1 , bison fiber claim 1 , wool claim 1 , bast fiber claim 1 , cellulose fibers or synthetic fiber.3. The method of claim 1 , wherein the insulation has a weight between 40 grams per square meter and 500 grams per square meter.4. The method of claim 1 , wherein the insulation is any of a batted ...

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Номер записи: 75
15-08-2019 дата публикации

WOOD ADHESIVES FOR PRODUCING PARTICLE BOARDS

Номер: US20190249046A1
Автор: LAGEL Marie-Christine, Pizzi Antonio, REDL Andreas
Принадлежит:

A composition based on phenolic resin comprising at least one phenolic resin, a vegetable protein hydrolysate, preferentially a wheat gluten hydrolysate, and an agent for accelerating curing, and also to the process for the production thereof. 1. A composition based on phenolic resin , characterized in that it comprises at least one phenolic resin , a vegetable protein hydrolysate , and an agent for accelerating curing.2. The composition of claim 1 , wherein the vegetable protein hydrolysate is a wheat gluten hydrolysate.3. The composition of claim 2 , wherein the agent for accelerating curing is selected from triacetin claim 2 , butyrolactone claim 2 , caprolactone and propylene carbonate.4. The composition as claimed in claim 1 , characterized in that the phenol and the wheat gluten hydrolysate are mixed in a phenol:wheat gluten hydrolysate ratio of 3.2:1 to 7.2:1 by weight.5. The composition as claimed in claim 1 , characterized in that the content of the agent for accelerating curing is between 4% and 10% by weight of the resin solids.6. The composition as claimed in claim 5 , characterized in that the content of the agent for accelerating curing is between 6% and 8% by weight of the resin solids.7. The composition as claimed in claim 1 , characterized in that the wheat gluten hydrolysate is obtained by acid or enzymatic hydrolysis.8. The composition as claimed in claim 1 , characterized in that the wheat gluten hydrolysate is obtained by enzymatic hydrolysis and has an average molecular weight of between 3 and 20 kDa.9. The composition as claimed in claim 8 , characterized in that the wheat gluten hydrolysate is obtained by enzymatic hydrolysis and has an average molecular weight of between 4 and 15 kDa.10. The composition as claimed in claim 1 , characterized in that the wheat gluten hydrolysate is also deamidated.11. A rigid phenolic foam board comprising a composition as claimed in .12. A particle or fiber board comprising a composition as claimed in .13. ...

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Номер записи: 76
06-08-2020 дата публикации

PREPARATION AND/OR FORMULATION OF PROTEINS CROSS-LINKED WITH POLYSACCHARIDES

Номер: US20200246504A1
Автор: Sommer-Knudsen Jens
Принадлежит:

Therapeutic compositions and/or formulations are provided, comprising: at least one cross-linked protein matrix, wherein the at least one cross-linked protein matrix comprises at least one protein residue and at least one saccharide-containing residue, and methods of producing the same. The cross-linked protein matrix may be derived from cross-linking a full length or substantially full length protein, such as tropoelastin, elastin, albumin, collagen, collagen monomers, immunoglobulins, insulin, and/or derivatives or combinations thereof, with a saccharide containing cross-linking agent, such as a polysaccharide cross-linking agent derived from, for example, hyaluronic acid or a cellulose derivative. The therapeutic compositions may be administered topically or by injection. The present disclosure also provides methods, systems, and/or kits for the preparation and/or formulation of the compositions disclosed herein. 1. A kit comprising:(a) a prefilled syringe, wherein the prefilled syringe is filled with a tissue compatible composition comprising a protein selected from the group consisting of tropoelastin and albumin; a hyaluronic acid cross-linking molecule comprising one or more carboxyl groups; and at least one intermolecular cross-linkage comprising an amide bond between an amine of the protein and a carboxyl group of the hyaluronic acid cross-linking molecule; and(b) instructions for use.2. The kit of claim 1 , further comprising an assortment of appropriate sized needles.3. The kit of claim 2 , wherein the assortment of appropriately sized needles comprise fine gauge needles.4. The kit of claim 3 , wherein the needles range from about 25 gauge to about 31 gauge.5. The kit of claim 2 , wherein the needles range from about 18 gauge to about 31 gauge.6. The kit of claim 1 , further comprising a needle delivery system.7. The kit of claim 6 , wherein the needle delivery system is selected from a needle roller ball type system claim 6 , an automatic injection pen ...

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Номер записи: 77
04-12-2014 дата публикации

Legume and/or oil seed flour-based adhesive composition

Номер: US20140352574A1
Автор: Dan Zhu, Srinivasan Damodaran
Принадлежит: WISCONSIN ALUMNI RESEARCH FOUNDATION

Adhesives made from phosphorylated legume and oil seed flours are described. The adhesive composition includes water and a legume and/or oil seed flour in which at least a portion of ε-amino moieties in lysine residues present in the flour are phosphorylated. An oxidizing agent may also optionally be added to the adhesive composition.

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Номер записи: 78
04-12-2014 дата публикации

Elastin-based copolymers and method of using

Номер: US20140356519A1
Автор: Mikael Trollsas, Syed F.A. Hossainy, Thierry Glauser, Yiwen Tang
Принадлежит: Abbott Cardiovascular Systems Inc

A copolymer comprising a block of an elastin pentapeptide and method of making and using the copolymer are provided.

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Номер записи: 79
22-08-2019 дата публикации

CO-CROSSLINKED HYALURONIC ACID-SILK FIBROIN HYDROGELS FOR IMPROVING TISSUE GRAFT VIABILITY AND FOR SOFT TISSUE AUGMENTATION

Номер: US20190255215A1
Автор: Cui Cunqi, Messina Darin J., Pavlovic Elizabeta, SMITHER Kate M., Yu Xiaojie
Принадлежит:

Hydrogels comprising a macromolecular matrix and water may be used to augment soft tissue of a human being, promote or support cell or tissue viability or proliferation, create space in tissue, and for other purposes. A macromolecular matrix may comprise a hyaluronic acid component crosslinked to a silk fibroin component. 1. A method of improving an aesthetic quality of an anatomic feature of a subject , the method comprising administering a hydrogel composition comprising hyaluronic acid , silk fibroin , and an ex vivo tissue to the subject , wherein the hyaluronic acid is crosslinked to the silk fibroin by a water-soluble coupling agent.2. The method of claim 1 , wherein the hydrogel composition is injected into the anatomic feature of the subject.3. The method of claim 1 , wherein the hydrogel composition is implanted into the anatomic feature of the subject.4. The method of claim 1 , wherein the ex vivo tissue comprises adipose tissue or fat tissue.5. The method of claim 1 , wherein the ex vivo tissue comprises adipose-derived progenitor cells.6. The method of claim 1 , wherein the water-soluble coupling agent comprises a multiamine crosslinker.7. The method of claim 1 , wherein the hydrogel composition has a weight ratio of the hyaluronic acid to the silk fibroin in the range of about 25:1 to about 1:1.8. The method of claim 1 , wherein the hyaluronic acid is present in the hydrogel composition in a concentration of about 16 mg/mL to about 20 mg/mL claim 1 , and wherein the silk fibroin is present in the hydrogel composition in a concentration of about 2 mg/mL to about 5 mg/mL.9. The method of claim 1 , wherein an appearance of the anatomic feature of the subject is improved.10. The method of claim 1 , wherein a tactile sensation of the anatomic feature of the subject is improved.11. The method of claim 1 , wherein the anatomic feature of the subject is augmented.12. A method of augmenting soft tissue of a human being comprising:providing a hydrogel composition ...

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Номер записи: 80
22-08-2019 дата публикации

LINKERS FOR PROTEIN INTERACTION PROFILING AND METHODS OF MAKING AND USING THE SAME

Номер: US20190256667A1
Автор: Bowman Christopher, Chapnick Douglas A., EBMEIER Christopher C., Gong Tao, Mcclure-Begley Tristan D., Old William, WORRELL Brady
Принадлежит:

Crosslinking compounds for effective and efficient cross-linking and identification of intermolecular and intramolecular interactions of proteins, peptides and nucleic acids. 135-. (canceled)36. A method of analyzing at least one protein , peptide , or nucleic acid comprising: {'br': None, 'sub': 'n', 'Z-Y-A-Y-Z'}, 'mixing a composition comprising at least one protein, peptide or nucleic acid with a crosslinker compound having the chemical structure n is an integer between 1 and 5;', 'A is an atom or compound;', 'each Z is independently a Michael acceptor (Z), and,', 'each Y is optional and is independently an organic compound optionally substituted with one or more heteroatoms selected from O, N, S, or P,, 'whereinto form a crosslinked sample;fragmenting the crosslinked sample to form fragments; anddetecting a mass-to-charge ratio of the fragments.37. (canceled)38. (canceled)39. The method of claim 36 , wherein the composition comprises at least one of a cell lysate claim 36 , cell culture claim 36 , and a tissue sample.4042-. (canceled)43. The method of claim 36 , wherein the mixing step is conducted at a pH between pH 5 and pH 10.4446-. (canceled)47. The method of claim 36 , further comprising purifying the crosslinked sample.48. (canceled)49. (canceled)50. The method of claim 36 , further comprising isolating the fragments.51. (canceled)52. (canceled)53. The method of claim 36 , wherein the fragmenting step comprises digesting the crosslinked sample with an enzymatic or chemical cleaving agent.54. The method of claim 53 , wherein the digesting comprises contacting the crosslinked sample with at least one of CNBr at pH 2 claim 53 , trypsin claim 53 , Glu-C endoproteinase claim 53 , pepsin claim 53 , restriction nuclease enzymes claim 53 , DNase I claim 53 , RNase α-sarcin claim 53 , and combinations thereof.55. The method of claim 36 , wherein the crosslinking compound comprises at least one collision-induced dissociation group and the method further comprises ...

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Номер записи: 81
29-08-2019 дата публикации

PROCESS FOR ISOLATING BIOACTIVE BIOMOLECULES FROM ANIMAL BY-PRODUCTS

Номер: US20190263891A1
Автор: Khiari Zied, Mason Beth
Принадлежит:

A process for producing a plurality of biomolecule products from by-products of animal food processing is described. The process includes the steps of mixing the by-products with one or more digestive enzymes in the presence of an acid to promote hydrolysis of the by-product to release the biomolecules, thereby providing a hydrolysis mixture. The hydrolysis mixture is subjected to a density-based fractional separation, thereby providing an oil fraction, a liquid fraction and a solid fraction. Then the liquid fraction is separated from the oil and solid fractions and filtered with a molecular mass cutoff filter, thereby providing a peptide product and a glycosaminoglycan product. The oil fraction may be further refined to provide an oil product and the solid fraction may be further processed to provide bone-derived products such as gelatin, ossein and collagen. 1. A process for producing a plurality of biomolecule products from by-products of animal food processing , the process comprising:mixing the by-products with one or more digestive enzymes in the presence of an acid to promote hydrolysis of the by-product to release the biomolecules, thereby providing a hydrolysis mixture;subjecting the hydrolysis mixture to a density-based fractional separation, thereby providing an oil fraction, a liquid fraction and a solid fraction; andseparating the liquid fraction from the oil and solid fractions and filtering the liquid fraction with a molecular mass cutoff filter, thereby providing a peptide product and a glycosaminoglycan product.2. The process of claim 1 , further comprising processing bone tissue contained in the solid fraction to generate one or more of or a combination of: a collagen product claim 1 , a gelatin product and an ossein product.3. The process of claim 1 , further comprising processing the oil to provide a refined oil product.4. The process of claim 1 , wherein the step of mixing the by-products further includes homogenization of the by-products.5. The ...

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Номер записи: 82
20-08-2020 дата публикации

Photo-coupled synergistically crosslinked hydrogel material and its composition, preparation method, use, product, and preparation kit

Номер: US20200262939A1
Автор: Chunyan BAO, Linyong ZHU, Qiuning LIN, Xuepeng Zhong, Yiqing Zhang, Yujie Hua
Принадлежит: Zhongshan Guanghe Medical Technology Co ltd

This invention provides a preparation, composition, product, and application of a photo-coupled synergistically crosslinked hydrogel material. The preparation includes dissolving Component A including a photosensitive polymer derivative having o-nitrobenzyl phototriggers and Component B including a polymer derivative having amine or alkene (double group) or sulfhydryl group in a biocompatible medium to obtain solution A and solution B, respectively; mixing the solution A and solution B homogeneously to obtain a hydrogel precursor solution; initiating photo-coupled synergistic crosslinking under an irradiation of a UV light to form the hydrogel. The irradiation causes the o-nitrobenzyl phototriggers to generate an aldehyde group/keto group or a nitroso group to initiate photo-coupled synergetic crosslinking. The photo-coupled synergistically crosslinked hydrogel has applications in tissue engineering, regenerative medicine, 3D printing and as a carrier of cell, protein or drug.

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Номер записи: 83
27-09-2018 дата публикации

Methods and Compositions for Synthesizing Improved Silk Fibers

Номер: US20180273693A1
Автор: Brendan TURNER, Daniel M. WIDMAIER, David N. Breslauer, Joshua KITTLESON, Lindsay WRAY
Принадлежит: Bolt Threads Inc

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

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Номер записи: 84
15-10-2015 дата публикации

POLYOLS FROM PROTEIN BIOMASS

Номер: US20150291741A1
Автор: Graiver Daniel, Hablot Elodie, Narayan Ramani
Принадлежит:

A process of producing multi-hydroxy polyols, the process comprising providing a biomass material and transamidating the biomass material with an amine selected from the group consisting essentially of a diamine and an alkanolamine, to provide low molecular weight derived amino-acids or derived oligomers of proteins. Thereafter, reacting the derived amino-acids or derived oligomers with a carbonate to provide hydroxyl terminated urethane oligomers. 2. A process of producing multi-hydroxy polyols as claimed in wherein claim 1 , in addition claim 1 , there is a step of alkoxylating any carbohydrates in the biomass to produce hydroxyl groups.3. A product produced by the process of .4. A product produced by the process of .5. A process as claimed in wherein the biomass material is a meal produced by the extraction of oil from vegetable seed.6. A process of producing multi-hydroxy polyols claim 1 , said process comprising:a. providing a material selected from a group consisting essentially of i. proteins, ii. amino acids derived from proteins, and, iii. mixtures of i. and ii.;b. transamidating any amino acids in said proteins or amino acids derived from said proteins with a material selected from the group consisting of diamine, triamine, an alkanolamine or a diol to produce amino and hydroxyl-terminated monomers;c. reacting said monomers with a carbonate to provide hydroxyl terminated urethane oligomers.7. A product produced by the process of .8. A process of producing multi-hydroxy polyols as claimed in wherein claim 6 , in addition claim 6 , there is a step d. of alkoxylating any carbohydrates in the protein to produce hydroxyl groups.9. A product produced by the process of .10. A polyurethane composition produced using the product claimed in .11. A polyurethane composition produced using the product claimed in .12. A polyurethane composition produced using the product claimed in .13. A polyurethane composition produced using the product claimed in .14. A polyurethane ...

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Номер записи: 85
06-10-2016 дата публикации

Pressure impulse mitigation

Номер: US20160290768A1
Автор: Leonid Tarasoff
Принадлежит: BioDynamic Armor Ltd

A pressure impulse mitigating barrier comprising a cross-linked gel comprising water and gelatin which has been crosslinked using (I) a functional silane compound comprising an electrophilic group (which is not a silane) and a group of formula (A) where R is H, a C 1-10 alkyl group, C 6-10 aryl group, or C 7-12 arylalkyl group; R′ is C 1-10 alkyl group or C 6-10 aryl group, or C 7-12 arylalkyl group; n is 0-2, with the proviso that at least one R is not H; said functional silane having an Mw of 800 g/mol or less; (II) a transition metal, lanthanide or Al compound; or (III) an enzyme such as transglutaminase.

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Номер записи: 86
20-10-2016 дата публикации

Protein-containing foams, manufacture and use thereof

Номер: US20160304688A1
Автор: Anthony A. Parker, Joseph J. Marcinko
Принадлежит: Evertree SAS

The invention relates generally to protein-containing polyurethane foams, methods and compositions for making the polyurethane foams, and articles comprising the polyurethane foams.

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Номер записи: 87
29-10-2015 дата публикации

Blends of oligopeptide terminal polyisobutylene or polystyrene

Номер: US20150307701A1
Автор: Emmanuel Croisier, Holger Frauenrath, SU Liang, Veronique Michaud
Принадлежит: Ecole Polytechnique Federale de Lausanne EPFL

Various blends of polymers are disclosed, comprising oligopeptide functionalised polymers such as polyisobutylene and polystyrene. Mono-functionalised and di-functionalised polymers (each containing 0 to 5 peptide units beyond its terminal amide group) may be blended with each other and/or with non-functionalised polymers to produce blended compositions. Such compositions are of use, for example, in vibrations dampers. Certain blends also exhibit self-healing properties.

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Номер записи: 88
18-10-2018 дата публикации

Methods and Compositions for Synthesizing Improved Silk Fibers

Номер: US20180298151A1
Автор: Breslauer David N., Kittleson Joshua, Turner Brendan, Widmaier Daniel M., Wray Lindsay
Принадлежит:

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers. 1. A fiber spinning solution , comprisinga polypeptide, the polypeptide comprising from 2 to 8 concatenated repeats of a recombinant silk polypeptide or circularly permuted variants thereof, the polypeptide further comprising a FLAG tag; anda spinning solvent, wherein said polypeptide is dissolved in said spinning solvent.2. The fiber spinning solution of claim 1 , wherein said spinning solvent comprises formic acid.3. The fiber spinning solution of claim 1 , wherein said spinning solvent is formic acid.4. The fiber spinning solution of claim 1 , wherein said fiber spinning solution comprises from 20-30% of said polypeptide by weight.5. The fiber spinning solution of claim 1 , wherein the FLAG tag is on the C-terminal end of the polypeptide.6. The fiber spinning solution of claim 1 , wherein said FLAG tag is a 3×FLAG tag.7. The fiber spinning solution of claim 1 , wherein said FLAG tag is encoded by a polynucleotide comprising SEQ ID NO: 1409.8. The fiber spinning solution of claim 1 , wherein the polypeptide comprises a property selected from the group consisting of an alanine composition from 12 to 40% claim 1 , a glycine composition from 25 to 50% claim 1 , a proline composition from 9 to 20% claim 1 , a β-turn composition from 15 to 37% claim 1 , a GPG amino acid motif content from 18 to 55% claim 1 , and a poly-alanine amino acid motif content from 9 to 35%.9. The fiber spinning solution of claim 1 , wherein said concatenated repeats comprise SEQ ID NO: 1396.10. A method of making a silk fiber claim 1 , comprising:{'claim-ref': {'@idref ...

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Номер записи: 89
25-10-2018 дата публикации

CROSS LINKED SILK-HYALURONIC ACID COMPOSITION

Номер: US20180303742A1
Автор: Manesis Nicholas J., Pavlovic Elizabeta, Serban Monica A., Yu Xiaojie
Принадлежит:

Compositions useful as dermal fillers and methods using such compositions to treat various skin and soft tissue conditions. The dermal fillers can comprise silk attached to hyaluronic acid using for example two cross linkers and can be used to treat of facial imperfections, facial defects, facial augmentations, breast imperfections, breast augmentations or breast reconstructions. 1. An injectable composition for treating a soft tissue condition , the composition comprising a gel comprising a silk crosslinked to a hyaluronic acid via amide bonding to a multiamine cross linker.2. The injectable composition of claim 1 , wherein the multiamine cross linker is (i) 3-[3-(3-amino propoxy)-2 claim 1 ,2-bis(3-amino-propoxymethyl)-propoxy]propylamine (4AA) or (ii) a diamine cross linker.3. The injectable composition of claim 2 , wherein the diamine cross linker is a linear diamine cross linker selected from the group consisting of a hexamethylene diamine (NMDA) claim 2 , lysine claim 2 , lysine methyl ester claim 2 , and lysine ethyl ester.4. The injectable composition of claim 1 , wherein the silk is a sericin-depleted silk fibroin having about 0% w/w to about 4% w/w of a native amount of sericin.5. The injectable composition of claim 1 , wherein the composition comprises a saline carrier claim 1 , and the gel is combined with the saline carrier in a formulation comprising one selected from the group consisting of about 1% to about 3% (w/v) gel with about 25% to about 50% (v/v) saline carrier; about 3% to about 5% (w/v) gel with about 20% to about 40% (v/v) saline; about 4% to about 6% (w/v) gel with about 20% to about 40% (v/v) saline; and about 6% to about 8% (w/v) gel with about 20% to about 30% (v/v) saline.6. The injectable composition of claim 1 , wherein the gel has a degree of crosslinking in the range of about 1% to about 15%.7. The injectable composition of claim 1 , wherein the hyaluronic acid is selected from the group consisting of sodium hyaluronan claim 1 , ...

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Номер записи: 90
03-11-2016 дата публикации

Methods of Modifying Agricultural Co-Products and Products Made Therefrom

Номер: US20160319083A1
Автор: Barry L. Mcgraw, Ramanathan S. Lalgudi, Robert J. Cain
Принадлежит: Battelle Memorial Institute Inc

In a method of producing a polymer composite, a polymer is provided in a liquid state such as a molten state. A plant material, such as soymeal, is provided that includes protein and carbohydrate. The plant material has a particle size less than 50 microns. A reactive protein denaturant is also provided. A dispersion of the plant material and the reactive protein denaturant is formed in a matrix of the liquid polymer. The plant material is reacted to bond with the reactive protein denaturant, and the reactive protein denaturant is reacted to bond with the polymer. The polymer is solidified to produce the polymer composite.

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Номер записи: 91
24-09-2020 дата публикации

BINDERS AND MATERIALS MADE THEREWITH

Номер: US20200299187A1
Автор: KISSELL Ronald E., SWIFT Brian Lee, XU Ruijian
Принадлежит:

Binders to produce or promote cohesion in non-assembled or loosely assembled matter. 1160.-. (canceled)161. A thermal or acoustical fiberglass insulation material comprising:(a) a collection of mineral fibers; and{'sup': 3', '3, '(b) a binder disposed on the collection of mineral fibers, wherein the binder consists essentially of i) at least one reaction product of a reducing sugar reactant and an amine reactant, ii) a silicon-containing coupling agent, and iii) optionally, a corrosion inhibitor, wherein the fiberglass material comprises less than about 99% by weight and more than about 75% by weight mineral fibers, and wherein the fiberglass material has a density of from about 0.4 lbs/ftto about 6 lbs/ft.'}162. The fiberglass material of claim 161 , wherein the amine reactant is an ammonium salt of a polycarboxylic acid.163. The fiberglass material of claim 161 , wherein the amine reactant is an ammonium salt of a monomeric polycarboxylic acid.164. The fiberglass material of claim 161 , wherein the density is from about 0.75 lbs/ftto about 2.5 lbs/ft.165. The fiberglass material of claim 161 , wherein the density is from about 2.25 lbs/ftto about 4.25 lbs/ft.166. The fiberglass material of claim 161 , wherein the density is from about 0.4 lbs/ftto about 1.5 lbs/ft.167. The fiberglass material of claim 161 , wherein the density is from about 3.75 lbs/ftto about 5.2 lbs/ft.168. A thermal or acoustical fiberglass insulation material comprising:(a) a collection of mineral fibers; and{'sup': 3', '3, '(b) a binder disposed on the collection of mineral fibers, the binder consisting essentially of i) a mixture of Maillard reactants, ii) a silicon-containing coupling agent, and iii) optionally, a corrosion inhibitor, wherein the fiberglass material comprises less than about 99% by weight and more than about 75% by weight mineral fibers, and wherein the fiberglass material has a density in the range from about 0.4 lbs/ftto about 6 lbs/ft.'}169. A thermal or acoustical ...

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Номер записи: 92
01-11-2018 дата публикации

Gelatin, chemically modified product thereof, aqueous composition and medical laminate containing same, production method for medical laminate, and cell sheet isolation method

Номер: US20180312726A1
Автор: Hirosuke Hatayama, Masataka Oda, Shunji Yunoki, Yoshimi Ohyabu, Yosuke Hiraoka
Принадлежит: Nitta Gelatin Inc, Tokyo Metropolitan Industrial Technology Research Instititute (TIRI)

A gelatin or a chemically modified product thereof contains 10 to 50% by mass of a high-molecular weight component, and a low-molecular weight component in such an amount that a value obtained by subtracting a low-molecular weight component content from a high-molecular weight component content is greater than or equal to 0% by mass.

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Номер записи: 93
09-11-2017 дата публикации

LINKERS FOR PROTEIN INTERACTION PROFILING AND METHODS OF MAKING AND USING THE SAME

Номер: US20170321016A1
Автор: Bowman Christopher, Chapnick Douglas A., EBMEIER Christopher C., Gong Tao, Mcclure-Begley Tristan D., Old William, WORRELL Brady
Принадлежит:

Crosslinking compounds for effective and efficient cross-linking and identification of intermolecular and intramolecular interactions of proteins, peptides and nucleic acids. 2. The crosslinking compound of claim 1 , wherein A is phosphorus (P) claim 1 , sulfur (S) claim 1 , nitrogen (N) claim 1 , oxygen (O) claim 1 , Calkyl optionally substituted with a reactive group or an affinity group claim 1 , cycloalkyl claim 1 , or heteroalkyl or heterocycloalkyl wherein the heteroatoms are selected from at least one of phosphorus (P) claim 1 , sulfur (S) claim 1 , nitrogen (N) claim 1 , or oxygen (O).36-. (canceled)7. The crosslinking compound of claim 2 , wherein A is pentane or hexane.8. The cross-linking compound of claim 1 , wherein the Michael acceptor (Z) is selected from the group consisting of a chemical moiety conjugated to a protein claim 1 , peptide or a nucleic acid claim 1 , an electron-deficient alkyne claim 1 , and a chemical moiety that forms a covalent bond with accessible ε-amino groups on lysine residues present in proteins or peptides.912-. (canceled)13. The cross-linking compound of claim 1 , comprising at least two (Z) and wherein at least one Michael acceptor (Z) is capable of forming a covalent bond with a protein or peptide and at least one Michael acceptor (Z) is capable of forming a covalent bond with a nucleic acid.14. The cross-linking compound of claim 1 , comprising at least two (Z) and wherein each Michael acceptor (Z) is the same chemical moiety.15. The cross-linking compound of claim 1 , comprising at least two (Z) and wherein each Michael acceptor (Z) is a unique chemical moiety.16. The cross-linking compound of claim 1 , wherein each Michael acceptor (Z) is selected from the group consisting of propiolate claim 1 , propiolamide claim 1 , yn-one claim 1 , ynethiolate claim 1 , acrylate claim 1 , vinylsulfone claim 1 , and propargylamide.17. The cross-linking compound of claim 1 , comprising an affinity handle (Q) that is a chemical moiety ...

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Номер записи: 94
24-10-2019 дата публикации

CYCLIC RGD CELL-BINDING MOTIF AND USES THEREOF

Номер: US20190322710A1
Автор: Hedhammar My
Принадлежит: SPIBER TECHNOLOGIES AB

A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for α5β1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface. 1. An immobilized protein or an immobilized peptide comprising: {'br': None, 'sup': 1', '1', '2', '3', '4', '5', '2, 'CXXRGDXXXC'}, 'a cell-binding motif with selectivity for integrins, wherein the cell-binding motif comprises an amino acid sequence as followswherein{'sup': 1', '2', '3', '4', '5, 'each of X, X, X, Xand Xare amino acid residues independently selected from the group consisting of: G, A, V, S, T, D, E, M, P, N and Q; and'}{'sup': 1', '2, 'Cand Care connected via a disulphide bond.'}2. The immobilized protein or the immobilized peptide according to claim 1 , wherein{'sup': 1', '3, 'each of Xand Xare amino acid residues independently selected from the group consisting of: G, S, T, M, N and Q; and'}{'sup': 2', '4', '5, 'each of X, Xand Xare amino acid residues independently selected from the group consisting of: G, A, V, S, T, P, N and Q.'}3. The immobilized protein or the immobilized peptide according to claim 2 , wherein{'sup': '1', 'Xis selected from the group consisting of: G, S, T, N and Q;'}{'sup': '3', 'Xis selected from the group consisting of: S, T and Q; and'}{'sup': 2', '4', '5, 'each of X, Xand Xare amino acid residues independently selected from the group consisting of: G, A, V, S, T, P and N.'}4. The immobilized protein or the immobilized peptide according to claim 3 , wherein claim 3 ,{'sup': '1', 'Xis S or T;'}{'sup': '2', 'Xis G, A or V;'}{'sup': '3', 'Xis S or T;'}{'sup': '4', 'Xis G, A, V or P; and'}{'sup': '5', 'Xis G, A or V.'}5. The immobilized protein or the immobilized peptide according to claim 1 , wherein Xis G or A.6. The immobilized protein or the immobilized peptide according to claim 2 , wherein Xis G.7. The immobilized protein or the ...

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Номер записи: 95
30-11-2017 дата публикации

Process for Preparing Tissue Regeneration Matrix

Номер: US20170342218A1
Автор: Gandhi Ankur, Kemnitzer John E., Kharge Angana, Panas Elaina, Saini Sunil
Принадлежит:

The present invention is directed to a process for making a tissue regeneration matrix. The process comprises providing a collagen-tropoelastin dispersion; freeze-drying the dispersion to provide a porous freeze-dried matrix; and then crosslinking the porous freeze-dried matrix. The present invention is also directed to a tissue regeneration matrix prepared by the process. 1. A process for making a tissue regeneration matrix , comprising:a) providing a collagen-tropoelastin dispersion;(b) freeze-drying the collagen-tropoelastin dispersion to provide a porous freeze-dried matrix; and(c) crosslinking the porous freeze-dried matrix.2. The process of claim 1 , wherein the dispersion comprises about 50 to about 99% collagen and about 1% to about 50% tropoelastin.3. The process of claim 1 , wherein the dispersion comprises about 70 to about 95% collagen and about 5% to about 30% tropoelastin.4. The process of claim 1 , wherein the dispersion comprises about 80 to about 95% collagen and about 5% to about 20% tropoelastin.5. The process of claim 1 , wherein the crosslinking step is performed by subjecting the matrix to an aldehyde solution.6. A tissue regeneration matrix prepared by the process of .7. A tissue regeneration matrix comprising collagen and elastin claim 1 , wherein the elastin is generated by crosslinking tropoelastin in the presence of collagen in vitro.8. A tissue regeneration matrix comprising collagen and tropoelastin claim 1 , prepared by a) providing a mixture of collagen and tropoelastin claim 1 , and b) crosslinking the mixture.9. The process of wherein the crosslinking step is performed by subjecting the matrix to a formaldehyde vapor.10. The process of further comprising the step of sterilizing the tissue regeneration matrix using electron beam irradiation or ethylene oxide.11. The process of further comprising the step of sterilizing the tissue regeneration matrix as a dry matrix using electron beam irradiation or ethylene oxide.12. The process of ...

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Номер записи: 96
14-11-2019 дата публикации

METHODS AND COMPOSITIONS FOR MAINTAINING THE CONFORMATION AND STRUCTURAL INTEGRITY OF BIOMOLECULES

Номер: US20190343912A1
Автор: Crow Brent B., Seifert Jennifer, Sood Paul R.
Принадлежит:

A composition includes a target pharmaceutical or biological agent, a solution containing the target pharmaceutical or biological agent, and substrate that is soluble in the solution. The substrate is capable of being solidified via a solidification process and the solidification process causes the substrate to become physically or chemically cross-linked, vitrified, or crystallized. As a result of the solidification process, particles are formed. The target pharmaceutical or biological agent within the solution retains proper conformation to ultimately produce a desired effect. 1. A method of forming particles that preserves biological activity of a pharmaceutical or biological agent when exposed to an organic solvent , the method comprising: a pharmaceutical or a biological agent; and', 'a substrate that is soluble in the solution, comprising one or more chemical species;, 'preparing a solution comprisingforming particles from the solution, wherein the forming comprises a process of at least one of physical or chemical cross-linking, vitrification, or crystallization of the substrate, or combinations thereof, and wherein the pharmaceutical or biological agent is entrapped by the formed particles; andwherein the biological activity of the pharmaceutical or biological agent is preserved following exposure of the particles to the organic solvent.2. The method according to claim 1 , further comprising:selecting the organic solvent; andmixing or suspending the particles in the organic solvent.3. The method according to claim 1 , wherein one or more of a surfactant claim 1 , a stabilizer claim 1 , or an emulsifier are incorporated into the substrate prior to claim 1 , during claim 1 , or after particle formation.4. The method according to claim 1 , wherein the particles are obtained by a process comprising:forming an emulsion of an aqueous phase dispersed in an organic phase, wherein the substrate and the pharmaceutical or biological agent comprise the dispersed aqueous ...

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Номер записи: 97
14-11-2019 дата публикации

HIGH DENSITY FIBROUS POLYMERS SUITABLE FOR IMPLANT

Номер: US20190343986A1
Автор: Ringeisen Timothy A., Wattengel William Christopher
Принадлежит:

This invention includes malleable, biodegradable, fibrous compositions for application to a tissue site in order to promote or facilitate new tissue growth. One aspect of this invention is a fibrous component that provides unique mechanical and physical properties. The invention may be created by providing a vessel containing a slurry, said slurry comprising a plurality of natural or synthetic polymer fibers and at least one suspension fluid, wherein the polymer fibers are substantially evenly dispersed and randomly oriented throughout the volume of the suspension fluid; applying a force, e.g., centrifugal, to said vessel containing said slurry, whereupon said force serves to cause said polymer fibers to migrate through the suspension fluid and amass at a furthest extent of the vessel, forming a polymer material, with said polymer material comprising polymer fibers of sufficient length and sufficiently viscous, interlaced, or interlocked to retard dissociation of said polymer fibers. 132-. (canceled)33. A composition suitable for implantation into a living being , comprising a network of randomly interlocked collagen fibers , wherein said collagen fibers are arranged with respect to a particulate to maintain said particulate within a spatial conformation within the composition , and retard disassociation of the particulate from the composition.34. The biocompatible composition of claim 33 , wherein said collagen fibers are arranged to support claim 33 , confine or lock said particulate within said spatial conformation and retard disassociation of said particulate from said biocompatible composition.35. The composition of wherein the network of fibers is initially in a dry form claim 33 , and upon being combined with a fluid claim 33 , results in a rehydrated implantable composition claim 33 , wherein the fluid is selected from at least one of blood claim 33 , bone marrow aspirates claim 33 , stem cells claim 33 , or concentrates thereof.36. The composition of claim ...

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Номер записи: 98
05-11-2020 дата публикации

BLENDS OF OLIGOPEPTIDE TERMINAL POLYISOBUTYLENE OR POLYSTYRENE

Номер: US20200347217A1
Автор: Croisier Emmanuel, Frauenrath Holger, Liang Su, Michaud Veronique
Принадлежит:

Various blends of polymers are disclosed, comprising oligopeptide functionalised polymers such as polyisobutylene and polystyrene. Mono-functionalised and di-functionalised polymers (each containing 0 to 5 peptide units beyond its terminal amide group) may be blended with each other and/or with non-functionalised polymers to produce blended compositions. Such compositions are of use, for example, in vibrations dampers. Certain blends also exhibit self-healing properties. 1. A polymer blend , comprising: a hydrophobic, flexible polymer having a glass transition temperature below 20° C. and only one monodisperse oligopeptide end group, the monodisperse oligopeptide end group having 1 to 5 amino acid repeating units; and', 'a hydrophobic, flexible polymer having a glass transition temperature below 20° C. and two monodisperse oligopeptide end groups; and, 'a first oligopeptide-terminal polymer component selected from the group consisting of a hydrophobic, flexible polymer that is different from said first oligopeptide-terminal polymer component and that has a glass transition temperature below 20° C. and only one monodisperse oligopeptide end group, the monodisperse oligopeptide end group having 1 to 5 amino acid repeating units; and', 'a hydrophobic, flexible polymer that is different from said first oligopeptide-terminal polymer component and that has a glass transition temperature below 20° C. and two monodisperse oligopeptide end groups., 'at least one additional polymer component selected from the group consisting of2. The polymer blend according to claim 1 , wherein at least one of said first oligopeptide-terminal polymer component and said at least one additional polymer component comprises repeating units selected from the group consisting of isobutylene claim 1 , butadiene claim 1 , siloxane claim 1 , acrylate claim 1 , and fluoropolymer units.3. The polymer blend according to claim 1 , wherein at least one of said first oligopeptide-terminal polymer component ...

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Номер записи: 99
13-12-2018 дата публикации

MATRICES COMPRISING A MODIFIED POLYSACCHARIDE

Номер: US20180355069A1
Автор: FORGET Aurelien, Shastri V. Prasad
Принадлежит:

The present invention discloses a matrix comprising a modified polysaccharide consisting of repeating disaccharide units whereby in at least 11% of the disaccharide units one primary alcohol group is oxidized into a carboxylic acid group. 119-. (canceled)20. A plastic surgery implant consisting essentially of a matrix comprising:a modified polysaccharide consisting of repeating disaccharide units, whereby in at least 11% of the disaccharide units one primary alcohol group is oxidized to a carboxylic acid group,wherein the matrix further comprises an unmodified polysaccharide component to adjust the mechanical properties of the matrix, and,{'sup': '7', 'wherein the shear modulus G′ of the matrix is in the range of from 10 Pa to 10Pa.'}21. The plastic surgery implant of claim 20 , wherein the modified polysaccharide is selected form the group consisting of a member of the carrageenean family claim 20 , hyaluronic acid claim 20 , heparin sulfate claim 20 , dermatan sulfate claim 20 , chondroitin sulfate claim 20 , alginate claim 20 , chitosan claim 20 , pullulan claim 20 , and agarose.22. The plastic surgery implant of claim 20 , wherein the modified polysaccharide is agarose.23. The plastic surgery implant of claim 20 , wherein the unmodified polysaccharide is selected from the group consisting of a member of the carrageenean family claim 20 , hyaluronic acid claim 20 , heparin sulfate claim 20 , dermatan sulfate claim 20 , chondroitin sulfate claim 20 , alginate claim 20 , chitosan claim 20 , pullulan claim 20 , and agarose.24. The plastic surgery implant of claim 20 , wherein the unmodified polysaccharide is agarose25. The plastic surgery implant of claim 20 , wherein the shear modulus G′ of the matrix is in the range of from 10 Pa to 100 kPa.26. The plastic surgery implant of claim 20 , at least 20-99% of the disaccharide units one primary alcohol group is oxidized to a carboxylic acid group27. The plastic surgery implant of claim 20 , wherein a weight ratio of the ...

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