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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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05-01-2012 дата публикации

Magnetic lysis method and device

Номер: US20120003654A2

Автор: Benjamin Hindson, Philip Belgrader

Принадлежит: Akonni Biosystems Inc, BIO-RAD QL Inc

A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.

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Номер записи: 1

27-04-2016 дата публикации

Составной биологический реактор установки для производства биомассы аэробных микроорганизмов

Номер: RU0000161691U1

Автор: Александр Иванович Сафонов

Принадлежит: Александр Иванович Сафонов

1. Составной биологический реактор установки для производства биомассы аэробных микроорганизмов, характеризующийся наличием ориентированной в вертикальной плоскости осесимметричной ферментационной емкости, оснащенной первой обечайкой, которая делит объем осесимметричной ферментационной емкости на первый подъемный и первый опускной каналы, наличием патрубка подвода в осесимметричную ферментационную емкость жидкой минеральной питательной среды, наличием патрубка подвода в нее же воздуха или кислорода, сопряженного с первым барботером, позиционированным в первом подъемном канале, наличием патрубка отвода наружу из осесимметричной ферментационной емкости накопленной биомассы, наличием патрубка первого подъемного и патрубка первого опускного каналов, наличием патрубка отвода из наджидкостного пространства осесимметричной ферментационной емкости паро-газовых продуктов метаболизма, наличием в первом опускном канале теплообменника, прикрепленного к внутренней стенке первой обечайки, наличием ориентированной в вертикальной плоскости, по крайней мере, одной осесимметричной ферментационной емкости-саттелита, оснащенной второй обечайкой, которая делит объем осесимметричной ферментационной емкости-саттелита на второй подъемный и второй опускной каналы, наличием патрубка подвода в осесимметричную ферментационную емкость-саттелит газообразного субстрата, сопряженного с размещенным во втором подъемном канале второго барботера, наличием продувочного патрубка, позиционированного на верхнем торце осесимметричной ферментационной емкости-саттелита, наличием патрубка второго подьемного и патр РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 161 691 U1 (51) МПК C12M 1/02 (2006.01) C12M 1/36 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ТИТУЛЬНЫЙ (21)(22) Заявка: ЛИСТ ОПИСАНИЯ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ 2014149047/10, 20.01.2016 (24) Дата начала отсчета срока действия патента: 20.01.2016 (72) Автор(ы): Сафонов Александр Иванович (RU) (73) Патентообладатель(и): Сафонов Александр ...

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Номер записи: 2

30-07-2019 дата публикации

Установка для выращивания хлореллы

Номер: RU0000191241U1

Автор: Алексей Юрьевич Торопов, Андрей Евгеньевич Новиков, Виктор Васильевич Мелихов, Людмила Алексеевна Птицына, Мария Васильевна Московец, Мария Викторовна Фролова

Принадлежит: Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт орошаемого земледелия" (ФГБНУ ВНИИОЗ)

Установка для выращивания хлореллы относится к сельскому хозяйству и может быть использована для высокопродуктивного и экологичного агро- и аквахозяйства, в частности для получения кормовых добавок, улучшения показателей гидрологического, гидрогеохимического и экологического состояния водных объектов. Установка для выращивания хлореллы, включающая установленный на каркасе биореактор хлореллы, представляющий собой аквариум и выполненный из светопропускающего материала, где биореактор содержит на боковой поверхности в нижнем основании штуцер для слива готовой суспензии, связанный линией отвода готовой суспензии в емкость готовой суспензии, и в верхнем основании штуцер для приготовления питательной среды, подачи питательного и маточного раствора, а также санитарного обслуживания, связанный линией подвода технологических потоков в аквариум, и систему их обслуживания, содержащую циркуляционные насосы и запорно-регулирующие устройства, отличающаяся тем, что аквариум выполнен открытым в форме желоба высотой 0,45-0,75 м, на боковой поверхности в верхнем основании которого смонтирован тангенциальный штуцер, связанный линией циркуляции со штуцером для слива готовой суспензии, обеспечивающий интенсивное перемешивание суспензии в аквариуме, при этом аквариум расположен под наклоном 3-5° в сторону слива готовой суспензии. В установке для выращивания хлореллы достигается снижение энергозатрат. 2 ил. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 191 241 U1 (51) МПК C12M 1/02 (2006.01) C12M 3/02 (2006.01) C12N 1/12 (2006.01) A01G 33/00 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ (52) СПК A01H 4/001 (2019.05); C12M 1/002 (2019.05); C12M 1/02 (2019.05); C12N 1/12 (2019.05); A01G 33/00 (2019.05) (21)(22) Заявка: 2019106940, 11.03.2019 11.03.2019 Дата регистрации: 30.07.2019 (73) Патентообладатель(и): Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт орошаемого земледелия" (ФГБНУ ...

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Номер записи: 3

12-11-2021 дата публикации

Устройство переработки пивной дробины в биогаз

Номер: RU0000207707U1

Автор: Борис Николаевич Федоренко, Владимир Владимирович Житков

Принадлежит: Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет пищевых производств"

Полезная модель относится к пищевой промышленности, биотехнологии и биоэнергетике, а именно к созданию усовершенствованного реактора переработки биомассы. Предложено устройство переработки отхода пивоваренного производства - пивной дробины в газообразное углеводородное топливо - биогаз. Полезная модель предусматривает создание реактора (ферментера) переработки дробины методом метанового брожения и оборудованного системой излучателей ультразвуковых волн для периодической ультразвуковой обработки субстрата пивной дробины с целью интенсификации процесса анаэробной переработки дробины микроорганизмами. Технический результат достигается оборудованием биореактора (ферментера) каскадом из системы ультразвуковых излучателей. Система размещения ультразвуковых излучателей на стенках внутренней поверхности ферментера представляет собой нисходящую спираль (рис 1), что позволяет при перемешивании субстрата пивной дробины, помещенного в ферментер, осуществлять наиболее полное и интенсивное воздействие энергией ультразвукового гидродинамического излучателя на весь объем обрабатываемого субстрата. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 207 707 U1 (51) МПК C12M 1/02 (2006.01) C12M 1/107 (2006.01) C12M 1/42 (2006.01) C02F 11/04 (2006.01) C12F 3/06 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ (52) СПК C12M 1/02 (2021.08); C12M 1/107 (2021.08); C12M 1/42 (2021.08); C02F 11/04 (2021.08); C12F 3/06 (2021.08) (21)(22) Заявка: 2021107419, 22.03.2021 22.03.2021 12.11.2021 Приоритет(ы): (22) Дата подачи заявки: 22.03.2021 (45) Опубликовано: 12.11.2021 Бюл. № 32 2 0 7 7 0 7 R U (54) УСТРОЙСТВО ПЕРЕРАБОТКИ ПИВНОЙ ДРОБИНЫ В БИОГАЗ (57) Реферат: Полезная модель относится к пищевой анаэробной переработки дробины промышленности, биотехнологии и микроорганизмами. Технический результат биоэнергетике, а именно к созданию достигается оборудованием биореактора усовершенствованного реактора переработки (ферментера) каскадом из системы биомассы. ...

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Номер записи: 4

31-01-2022 дата публикации

Устройство для выделения и очистки нуклеиновых кислот и/или макромолекул из биологического образца

Номер: RU0000209043U1

Автор: Александр Николаевич Неваленный, Анна Леонидовна Ясенявская, Гиоргий Александрович Ростошвили, Игорь Юрьевич Алексанян, Кристина Шотаевна Арнаудова, Любовь Михайловна Титова, Марина Александровна Самотруева, Мария Романовна Копылова, Ольга Александровна Башкина, Юрий Александрович Максименко

Принадлежит: Федеральное государственное бюджетное образовательное учреждение высшего образования "Астраханский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО Астраханский ГМУ Минздрава России), Федеральное государственное бюджетное образовательное учреждение высшего образования Астраханский государственный технический университет, ФГБОУ ВО "АГТУ"

Полезная модель относится к области молекулярной биологии, биотехнологии и биохимии и может быть использована в медицине. Полезная модель представляет собой устройство для выделения и очистки магнитных наночастиц при экстракции и очистке нуклеиновых кислот (ДНК и РНК) сорбентным методом из различного биологического материала.Технический результат - повышение эффективности работы устройства при его использовании.Устройство имеет верхнюю и нижнюю пластины, держатель пробирок с ручками для переноски, расположенный на верхней пластине, который имеет отверстия и оптические окна, неподвижно закрепленные на нижней пластине вертикальные стойки, оснащенные фиксаторами для ограничения движения по ним в вертикальной плоскости верхней пластины, на стойках жестко фиксированы две горизонтальные оси, с горизонтальными осями связана движущаяся по ним направляющая с жестко закрепленными на ней магнитами в форме стержней, каждый магнит снабжен жестко закрепленной на нем защитной крышкой из пластикового коррозионностойкого материала, держатель пробирок выполнен из отдельных сегментов, имеющих один ряд отверстий для пробирок. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 209 043 U1 (51) МПК C12M 1/00 (2006.01) C12N 15/10 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ (52) СПК C12M 1/00 (2021.08); C12N 15/10 (2021.08) (21)(22) Заявка: 2021125218, 25.08.2021 (24) Дата начала отсчета срока действия патента: Дата регистрации: 31.01.2022 (45) Опубликовано: 31.01.2022 Бюл. № 4 2 0 9 0 4 3 R U (73) Патентообладатель(и): Федеральное государственное бюджетное образовательное учреждение высшего образования Астраханский государственный технический университет, ФГБОУ ВО "АГТУ" (RU), Федеральное государственное бюджетное образовательное учреждение высшего образования "Астраханский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО Астраханский ГМУ Минздрава России) (RU) (56) Список документов, ...

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Номер записи: 5

15-03-2012 дата публикации

Energy photoconverter for obtaining biofuels

Номер: US20120064589A1

Автор: Bernard A. J. Stroiazzo-Mougin, Cristian Gomis Catala

Принадлежит: Individual

The present invention relates to the design of energy photoconverters which act in a continuous and closed manner for producing biofuels and other products of interest by means of the mass culturing of phytoplankton.

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Номер записи: 6

03-05-2012 дата публикации

Apparatus for synthesizing oligonucleotides and methods of use

Номер: US20120107181A1

Автор: Francis J. Ring, Isaiah E. Cedillo, Max N. Moore

Принадлежит: ISIS PHARMACEUTICALS INC

The present invention relates to apparatus and methods for synthesizing oligonucleotides wherein a reaction zone has a variable volume based on the position of a piston, and the piston is adjusted to control the amount of headspace above solid support in the reaction zone. Methods and apparatus that limit the size or existence of the headspace are described.

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Номер записи: 7

31-05-2012 дата публикации

Bioreactor Comprising A Silicone Coating

Номер: US20120135514A1

Автор: Christoph MÜLLER-REES, Rupert Pfaller

Принадлежит: Wacker Chemie AG

The invention relates to a bioreactor for cultivating phototrophic organisms in an aqueous culture medium. The reactor parts and/or fittings that come into contact with the culture medium are entirely or partially coated with a silicone layer, and the surface of the silicone layer has a contact angle to the water of at least 100°.

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Номер записи: 8

21-06-2012 дата публикации

Disposable vortex breaker

Номер: US20120155216A1

Автор: David DeCoste, Martin Morrissey

Принадлежит: EMD Millipore Corp

The present invention consists of a closed presterilized bag having a disposable mixing element within it, a drive mechanism outside of the bag for rotating the mixing element without voiding sterility and a vortex breaker in the torm of one or more plastic sheet materials that are attached to various inner surfaces of the bag and disrupt the formation of vortices within the bag. Preferably the sheet(s) are formed of the same material as the bag and are sealed to the bag surfaces. More preferably, the sheet(s) extend across a diameter of the bag. Most preferably, the sheet(s) are perforated with one or more slits or openings to allow for good flow and mixing without a vortex being formed.

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Номер записи: 9

28-06-2012 дата публикации

Renewable chemicals and fuels from oleaginous yeast

Номер: US20120164701A1

Автор: Anna Coragliotti, Anthony G. Day, Chung-Soon Im, Donald E. Trimbur, Harrison F. Dillon, Scott Franklin

Принадлежит: Solazyme Inc

The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.

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Номер записи: 10

19-07-2012 дата публикации

Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner

Номер: US20120184009A1

Автор: Eudes Francois Marie De Crecy

Принадлежит: Individual

A method and device for growing plant, animal or stem cells in a continuous manner.

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Номер записи: 11

18-10-2012 дата публикации

Sugar mixtures and methods for production and use thereof

Номер: US20120264873A1

Автор: Aharon Meir Eyal, Asher Vitner, Revital Mali, Robert P. Jansen

Принадлежит: Individual

A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio ≧0.06 to total saccharides; disaccharides in a ratio to total saccharides ≧0.05; pentose in a ratio to total saccharides ≧0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures.

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Номер записи: 12

02-05-2013 дата публикации

Biological information processing method and device, recording medium and program

Номер: US20130110409A1

Автор: Kazuhiro Sakurada

Принадлежит: Sony Corp

Provided is a biological information processing method and a device, a recording medium and a program that are able to predict and control changes in the state of an organism. The expression level of molecules in an organism is measured over a specific time interval; the measured time-series data is divided into a periodic component, an environmental stimulus response component and a baseline component; constant regions of the time-series data are identified from variations in the baseline component or from the amplitude or periodic variations of the periodic component; and causal relation between the identified constant regions is identified. The relation between the external environment and variations in the internal environment is identified and from the identified causal relation between the constant regions, changes in the state of the organism are inferred.

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Номер записи: 13

09-05-2013 дата публикации

THERMO-CONDUCTIVE CELL CULTURE DISH HOLDER

Номер: US20130115691A1

Автор: Schryver Brian

Принадлежит: BIOCISION, LLC

The present invention describes various devices for holding cell culture dishes in a secure manner and to ensure rapid and uniform heat transfer to and from the cell culture dish. 1. A device for cooling or warming of a cell culture dish , wherein the device is thermo-conductive , wherein the device comprises a top surface and a bottom surface , wherein the top surface comprises one or more recessed areas , wherein the one or more recessed areas engage one or more projections present on an underside of the cell culture dish , and wherein engagement of the one or more recessed areas and the one or more projections stabilizes the cell culture dish on the top surface of the thermo-conductive device and provides an increased area of direct contact between the undersurface of the cell culture dish and the top surface of the device.2. The device of claim 1 , wherein the cell culture dish is a non-circular cell culture dish.3. The device of claim 1 , wherein the cell culture dish is a circular cell culture dish.4. The device of claim 3 , wherein the circular cell culture dish has a diameter selected from the group consisting of 35 mm claim 3 , 50 mm claim 3 , 60 mm claim 3 , 80.5 mm claim 3 , 92 mm claim 3 , 100 mm claim 3 , and 150 mm.5. The device of claim 3 , wherein the circular cell culture dish is a Corning culture dish.6. The device of claim 3 , wherein the circular cell culture dish is a Petri dish.7. The device of claim 6 , wherein the Petri dish is a Corning Petri dish.8. The device of claim 1 , wherein the device comprises a metal.9. The device of claim 8 , wherein the metal is selected from the group consisting of copper and aluminum.10. The device of claim 1 , wherein the device comprises a metal alloy.11. The device of claim 10 , wherein the metal alloy is selected from the group consisting of a copper alloy and an aluminum alloy.12. The device of claim 1 , wherein the one or more recessed areas comprise one or more ring-shaped recess channels.13. The device ...

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Номер записи: 14

16-05-2013 дата публикации

Device for automatically analyzing nucleic acid

Номер: US20130122576A1

Автор: Oh Won Kwon

Принадлежит: Korea Institute of Machinery and Materials KIMM

An apparatus for automatically analyzing a nucleic acid includes: a sample preprocessing device including a plurality of chambers in which reagents mixed with a sample are accommodated according to sample preprocessing process order for extracting a nucleic acid from the sample; and a nucleic amplifying and detecting device connected with the sample preprocessing device to receive the nucleic acid extracted from the sample.

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Номер записи: 15

20-06-2013 дата публикации

Magnetic lysis method and device

Номер: US20130157274A1

Автор: Benjamin Hindson, Phillip Belgrader

Принадлежит: Akonni Biosystems Inc, BIO-RAD QL Inc

A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.

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Номер записи: 16

15-08-2013 дата публикации

Methods and Systems for the Production of Hydrocarbon Products

Номер: US20130210096A1

Автор: James Obern, Michael Anthony Schultz, Sean Dennis Simpson

Принадлежит: Lanzatech New Zealand Ltd

Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.

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Номер записи: 17

12-09-2013 дата публикации

V-Trough Photobioreactor System and Method of Use

Номер: US20130232866A1

Автор: Carl L. White, Jason D. LICAMELE

Принадлежит: Heliae Development LLC

Disclosed herein are photobioreactor systems for high productivity aquaculture or aquafarming for growing of algae or other organisms in an aquatic environment featuring aspects that favor improved growth rates by achieving control over the contents of the growth medium, including carbon source, nitrogen source, and essential trace elements necessary for growth.

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Номер записи: 18

07-11-2013 дата публикации

Downstream bioprocessing device

Номер: US20130296538A1

Автор: Sarfaraz K. Niazi

Принадлежит: Therapeutic Proteins International LLC

Large-scale downstream processing of secreted recombinant proteins is provided in a single device, wherein the contents of a plurality of bioreactors are combined simultaneous to their harvesting and purification resulting in significant savings of time and the cost of manufacturing.

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Номер записи: 19

20-02-2014 дата публикации

Reaction container and method for producing same

Номер: US20140048540A1

Автор: Hideji Tajima

Принадлежит: UNIVERSAL BIO RESEARCH CO LTD

The invention relates to a reaction container and a method for producing the reaction container, and provides a low-cost reaction container which is capable of consistently, quickly, and efficiently performing a process that includes a reaction such as extraction or amplification of a nucleic acid, while saving user's trouble without increasing the scale of the device. The reaction container is configured to comprise: one or two or more housing parts for reactions comprising: a narrow-mouthed piping part in which a reaction reagent or a portion thereof is housed or is housable; a wide-mouthed piping part that is communicated with the narrow-mouthed piping part and provided on an upper side of the narrow-mouthed piping part, and has an aperture that is wider than an aperture of the narrow-mouthed piping part; and a punchable film provided such that it partitions an interval between the wide-mouthed piping part and the narrow-mouthed piping part.

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Номер записи: 20

27-03-2014 дата публикации

Microbial fuel cell comprising a microprobe array

Номер: US20140087213A1

Автор: Jae-Hyoung Park, Seung-Ki Lee

Принадлежит: Industry Academic Cooperation Foundation of Dankook University

Provided is a microbial fuel cell (MFC). The MFC includes a microfluidic element having an inlet portion and an outlet portion for intake and discharge of a culture fluid containing cells and a microchannel portion for capturing the cells and interconnecting the inlet portion and the outlet portion, a microprobe-array element having microprobes as anodes for extracting electrons produced during a metabolic process of the cells, and delivering the extracted electrons to an external circuit outside the cells, and a cathode for delivering the electrons used in the external circuit to an electron acceptor outside the cells. The microprobes penetrate the microfluidic element and are inserted into a plurality of single cells captured by the microchannel portion when the microfluidic element and the microprobe-array element are coupled together. The microprobes are separated from the single cells when the microfluidic element and the microprobe-array element are separated from each other.

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Номер записи: 21

02-01-2020 дата публикации

Droplet digital pcr chip

Номер: US20200001301A1

Автор: Jian Shi, Shuanghong Xiang, Xiaohui Song

Принадлежит: Pilot Gene Technologies (hangzhou) Co Ltd

The present invention discloses a droplet digital PCR chip. The droplet digital PCR chip includes at least one chip unit, each chip unit includes a chip body formed by bonding a top piece and a bottom piece, the chip body is internally provided with an inlet chamber, a droplet storage chamber, and an injection hole. The injection hole connects with the inlet chamber, a plurality of droplet generating channels are disposed between the inlet chamber and the droplet storage chamber, a height of the droplet generating channel is smaller than a height of the droplet storage chamber, an injection fluid is injected into the inlet chamber through the injection hole, and the injection fluid is emulsified and enters the droplet storage chamber at a junction of the droplet generating channels and the droplet storage chamber.

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Номер записи: 22

03-01-2019 дата публикации

Chip and application thereof

Номер: US20190001327A1

Автор: Ping Wu, QIN Yan

Принадлежит: Direct Genomics Co Ltd

Disclosed is a chip. The chip comprises a substrate (1) and a base layer (2) in pressing arrangement with the substrate; the substrate comprises a first surface (1a) and a second surface (1b) in opposite arrangement, reaction tank arrays formed by a plurality of flowing channels (11) are arranged on the first surface of the substrate at intervals, two oppositely arranged side walls (111, 112) of each flowing channel (11) stretch along the length direction of the flowing channel (11) and intersect at two ends of the flowing channel to form two tapered tail ends (113) with included angles, and a fluid inlet hole (12) and a fluid outlet hole (13) which are communicated with the second surface of the substrate are respectively provided on the surfaces of the two tapered tail ends (113); and the base layer (2) comprises a transparent base (21) and a spacing layer (22) arranged on the surface of the transparent base, the spacing layer (22) is in contact with the firs surface (1a) of the substrate, and a corrosion groove is provided on the spacing layer (22) corresponding to a position where the flowing channel (11) is located. The flow field distribution of the chip is good, the deformation rate of a base in the chip is low, and the fluid in the chip can be fully flushed or replaced. Also disclosed is an application of the chip.

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Номер записи: 23

04-01-2018 дата публикации

NUCLEIC ACID AMPLIFICATION METHOD AND NUCLEIC ACID AMPLIFICATION APPARATUS

Номер: US20180002737A1

Автор: UEHARA Masayuki

Принадлежит:

A nucleic acid amplification method includes a step of heating a first region of a container housing a droplet containing a target nucleic acid and a sample necessary for amplification of the target nucleic acid to a denaturation temperature of the target nucleic acid and heating a second region different from the first region to a synthesis temperature of the target nucleic acid, and an amplification step of repeating a cycle through a denaturation stage at which the droplet housed in the container is moved to and retained in the first region and a synthesis stage at which the droplet is moved to and retained in the second region at a plurality of times. At the amplification step, periods of part of cycles of the plurality of cycles are made shorter than periods of the other cycles. 1. A nucleic acid amplification method comprising:a heating step of heating a first region of a container housing a droplet containing a template nucleic acid and a sample necessary for amplification of a target nucleic acid in the template nucleic acid to a denaturation temperature of the target nucleic acid and heating a second region different from the first region to a synthesis temperature of the target nucleic acid; andan amplification step of repeating a cycle through a denaturation stage at which the droplet housed in the container is moved to and retained in the first region and a synthesis stage at which the droplet is moved to and retained in the second region at a plurality of times,wherein, at the amplification step, periods of part of cycles of the plurality of the cycles are made shorter than periods of the other cycles.2. The nucleic acid amplification method according to claim 1 , wherein claim 1 , at the amplification step claim 1 , periods of the respective cycles following the time as a specified-numbered time counted from the first time are made shorter than periods of the respective cycles until the time as the specified-numbered time counted from the first time.3. ...

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Номер записи: 24

07-01-2021 дата публикации

Integrated Continuous Isolation of Fluid Streams From Sterile Process Vessels

Номер: US20210002601A1

Автор: Robert Snow

Принадлежит: Genzyme Corp

Provided herein are isolation processes and the associated hardware to allow fluid streams to be isolated from a sterilized system (e.g., a sterile process vessel) that contains a sterile process. The isolation processes described herein allow for continuous removal of fluid streams (e.g., waste streams, liquid containing recombinant therapeutic proteins) from a sterilized system (e.g., a biological manufacturing system), which provides for less manual manipulation of the sterilized system and a decreased risk of contaminating the sterilized system.

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Номер записи: 25

11-01-2018 дата публикации

ACTIVE SUBSTANCE FOR PREVENTING DEGENERATION OF HEARING, COMPOSITION COMPRISING SAME, AND PREPARATION METHOD THEREOF

Номер: US20180007836A1

Автор: Chan Yin-Ching, CHEN Chin-Chu, Chen Wan-Ping, Lee Li-Ya

Принадлежит: GRAPE KING BIO LTD.

The present invention discloses the active substances for preventing hearing deterioration, its preparation method, the pharmaceutical composition containing the active substances, and the preparation method of the pharmaceutical composition. The preparation method of the active substances is performed by plate cultivation, flask cultivation and fermentation tank cultivation, to obtain the active substances of mycelia in powder form. The powder of mycelia is proved to have the effect of preventing hearing deterioration. 113.-. (canceled)14Hericium erinaceus. A pharmaceutical composition for preventing a hearing deterioration , comprising an active substance of a , and one selected from the group consisting of a biologically acceptable carrier , an excipient , a diluent and an adjuvant , wherein the active substance is prepared by a method comprising:{'i': 'H. erinaceus', '(a) inoculating an mycelium on an agar plate to be incubated;'}(b) inoculating the incubated mycelium in step (a) into a first medium on a small scale to be incubated; and(c) inoculating the incubated mycelium in step (b) into a second medium on a large scale to be incubated to obtain a fermented medium containing the active substance.15. The pharmaceutical composition according to claim 14 , wherein the incubation in step (a) is performed at 15-32° C. for 8-16 days.16. The pharmaceutical composition according to claim 14 , wherein the incubation in step (b) is performed at 20-30° C. claim 14 , pH 4.5-6.5 claim 14 , and a shaking rate of 100-250 rounds per minutes (rpm) for 3-5 days.17. The pharmaceutical composition according to claim 14 , wherein in step (c) claim 14 , the second medium on the large scale is accommodated in a fermentation tank having a tank pressure of 0.8-1.2 kg/cmand a stirring rate of 10-150 rpm claim 14 , and a gas is introduced into the fermentation tank at an aeration rate of 0.5-1 volume per volume per minute (vvm).18. The pharmaceutical composition according to claim 17 , ...

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Номер записи: 26

11-01-2018 дата публикации

Freezing bag container

Номер: US20180007890A1

Автор: Masaki MATSUMURA

Принадлежит: Terumo Corp

A container for containing a freezing bag filled with biological tissue and for cooling and warming the freezing bag. The container includes a main body possessing an inner surface, a first side surface and a second side surface positioned opposite the first side surface. The main body is substantially rectangular parallelepiped shaped. The container includes at least one opening in at least one of the first side surface and the second side surface of the container, and at least two ridges spaced apart from one another on the inner surface of the container to create an air gap between the spaced apart ridges, the inner surface of the container and the outer surface of the freezing bag.

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Номер записи: 27

11-01-2018 дата публикации

Bioreactor With Higher Agitation Rates

Номер: US20180010082A1

Автор: "Costa Rita DOrnelas P. De Barros", Beaney Anthony, Jaques Colin Mark, Khan Mohsan Waseem, VALENTINE David

Принадлежит:

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor. 1. A bioreactor comprising:a bioprocess container having a top, a bottom, and at least one side wall therebetween, the bioprocess container defining a hollow enclosure for receiving a culture medium;a mixing device comprising a rotatable shaft coupled to at least one impeller; anda motor coupled to the rotatable shaft for rotating the shaft and the at least one impeller, the motor being configured to rotate the impeller at a rate of from 80 rpm to 200 rpm.2. A bioreactor as defined in claim 1 , wherein the mixing device comprises at least one top impeller and at least one bottom impeller that extend into the hollow enclosure of the bioprocess container.3. A bioreactor as defined in claim 2 , wherein the top impeller and the bottom impeller are made from a polymer material claim 2 , the top impeller and the bottom impeller having a hydrophilic surface.4. A bioreactor as defined in claim 1 , further comprising at least one baffle being configured to extend adjacent to the side wall of the ...

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Номер записи: 28

11-01-2018 дата публикации

LIGAND-BINDING FIBER AND CELL CULTURE SUBSTRATE USING SAID FIBER

Номер: US20180010115A1

Автор: AIHARA Ayako, Iwamoto Shunsuke, Kishioka Takahiro, Nishino Taito, Sakuma Daisuke, Umezaki Makiko

Принадлежит: NISSAN CHEMICAL INDUSTRIES, LTD.

The invention provides a ligand-bonded fiber in which a ligand having affinity for a cell membrane receptor is immobilized on a fiber precursor, and a cell culture substrate capable of repeating ex vivo amplification of a cell expressing a cell membrane receptor by using the ligand-bonded fiber. 1. A ligand-bonded fiber comprising a ligand having affinity for a cell membrane receptor , and a fiber precursor bonded to the ligand , thus forming a ligand-bonded fiber precursor (hereinafter to be referred to as a fiber precursor).2. The ligand-bonded fiber according to claim 1 , wherein the above-mentioned cell membrane receptor is a thrombopoietin (TPO) receptor.4. The ligand-bonded fiber according to claim 3 , wherein the above-mentioned ligand has an amino group claim 3 , and the amino group and the above-mentioned Qare bonded.6. The ligand-bonded fiber according to claim 3 , wherein the fiber precursor further comprises a crosslinking agent and an acid compound.7. The ligand-bonded fiber according to claim 3 , wherein the above-mentioned fiber precursor is produced by spinning a composition for producing a fiber precursor claim 3 , which composition comprising the above-mentioned polymer compound claim 3 , a crosslinking agent claim 3 , an acid compound and a solvent.8. The ligand-bonded fiber according to claim 7 , wherein the above-mentioned fiber precursor is produced by spinning the above-mentioned composition for producing a fiber precursor claim 7 , on a surface-treated substrate.9. The ligand-bonded fiber according to claim 3 , wherein the above-mentioned polymer compound has a weight average molecular weight of 1 claim 3 ,000-1 claim 3 ,000 claim 3 ,000.10. The ligand-bonded fiber according to claim 1 , wherein the above-mentioned fiber precursor is produced by heating at 70-300° C.12. The ligand-bonded fiber according to claim 11 , wherein Xis a 4-t-butylphenyl group claim 11 , and Xis an amino group.13. A cell culture substrate comprising the ligand-bonded ...

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Номер записи: 29

10-01-2019 дата публикации

Cell sheet embedding agent, cell sheet-containing composition, and kit

Номер: US20190010214A1

Автор: Kentaro Nakamura, Shinya Sano, Tadashi Sameshima, Yosuke Kuruma

Принадлежит: Fujifilm Corp, Terumo Corp

In the formula, X and Y each independently represent an amino acid, m is an integer of 2 to 10, n is an integer of 3 to 100, and A and B each represent any amino acid or any amino acid sequence.

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Номер записи: 30

09-01-2020 дата публикации

Processes and bioreactors for gas fermentation products

Номер: US20200010861A1

Автор: Jian Yu, Pradeep Munasinghe

Принадлежит: UNIVERSITY OF HAWAII

A process for producing at least one polyhydroxyalkanoate through gas fermentation may include: providing at least one gas fermentation vessel having a volume partially filled with a liquid fermentation broth that includes: water, suspended gas-fermenting microorganisms capable of producing the at least one polyhydroxyalkanoate, and nutrients for the gas-fermenting microorganisms, a remaining part of the volume of the at least one gas fermentation vessel being filled with a gas phase; continuously withdrawing an aliquot of the liquid fermentation broth from the at least one gas fermentation vessel; supplying a gaseous substrate comprising CO 2 , H 2 , and O 2 ; cultivating the gas-fermenting microorganisms to form a cell mass containing the at least one polyhydroxyalkanoate; and recovering the at least one polyhydroxyalkanoate from the cell mass. A gas fermentation bioreactor may include: a vessel, at least one effluent circulation conduit communicating with the vessel interior, at least one spraying nozzle, and a feeding system.

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Номер записи: 31

14-01-2021 дата публикации

COMPONENT ANALYSIS DEVICE, DRUG COMPONENT ANALYSIS DEVICE, COMPONENT ANALYSIS METHOD, AND DRUG COMPONENT ANALYSIS METHOD

Номер: US20210011003A1

Автор: Kanda Katsuhiro, Shimizu Yusuke, Takahashi Ryosuke

Принадлежит:

Provided is a component analysis device, which comprises an analysis unit for measuring a component fed to the plurality of containers and analyzing the component thus measured, wherein the plurality of containers are at least a first container and a second container, wherein the first container retains a first solution containing a substance that releases adhesion between multiple cells forming the liver cell tissue, and the second container retains a buffer solution, and wherein the analysis unit measures an amount of the component discharged from the liver cell tissue in the first container into the first solution and an amount of the component discharged from the liver cell tissue in the second container into the buffer solution in the second container, and analyzes an amount of the component to be discharged via a bile duct in the liver cell tissue. 1. A component analysis device , comprisinga retention unit for retaining a plurality of containers for retaining a liver cell tissue, andan analysis unit for measuring a component fed to the plurality of containers and analyzing the component thus measured,wherein the plurality of containers are at least a first container and a second container,wherein the first container retains a first solution containing a substance that releases adhesion between multiple cells forming the liver cell tissue,wherein the second container retains a buffer solution, andwherein the analysis unit:measures an amount of the component discharged from the liver cell tissue in the first container into the first solution and an amount of the component discharged from the liver cell tissue in the second container into the buffer solution in the second container, andanalyzes an amount of the component to be discharged via a bile duct in the liver cell tissue.2. The component analysis device according to claim 1 , further comprising a temperature regulation unit for regulating a temperature of a liquid in the plurality of containers claim 1 , ...

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Номер записи: 32

03-02-2022 дата публикации

DRIVE MECHANISM CAPABLE OF DEALING WITH GAS STERILIZATION

Номер: US20220033201A1

Автор: YAMASAKI Yukito

Принадлежит:

Provided is a drive mechanism capable of withstanding sterilization treatment using a sterilization gas such as hydrogen peroxide and capable of performing positioning operation with heightened accuracy. In a drive mechanism according to the invention, a movable block disposed in the internal space of a partition wall for blocking a sterilization gas is coupled to a movable platform disposed in the external space of the partition wall by means of a magnet coupling mechanism. Further, biasing magnet units are disposed on surfaces, of the movable block and the movable platform, on which no magnet coupling mechanism is disposed, such that the biasing magnet units serve as magnetic poles repelling each other. Repelling force generated by the biasing magnet units serves as biasing force that biases the movable platform toward the partition wall. 1. A drive mechanism comprising:a movable block;a drive unit moving the movable block;a guide mechanism guiding the movable block in a predetermined direction;a partition wall isolating the movable block, the drive unit, and the guide mechanism from an external environment;a movable platform provided outside the partition wall and at a position facing the movable block via the partition wall so as to cover at least a part of the partition wall and be movable along the partition wall;a first magnet coupling mechanism including magnets attracting each other on surfaces (first surfaces) mutually facing the movable block and the movable platform via the partition wall; anda second magnet coupling mechanism including magnets attracting each other and provided on surfaces (second surfaces) of the movable block and the movable platform orthogonal to the first surfaces and facing each other via the partition wall,wherein the movable platform moves on a trajectory guided by the partition wall by following the movement of the movable block.2. The drive mechanism according to claim 1 , comprising a rolling body rollably attached to the ...

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Номер записи: 33

18-01-2018 дата публикации

Thermal cycler for biological material reaction, biological material analysis apparatus, system for controlling rate of temperature change in biological material reaction, method for controlling rate of temperature change in biological material reaction, biological material analysis method, and temperature control program for biological material reaction

Номер: US20180015476A1

Автор: Tatsuo Maeda

Принадлежит: Sony Corp

A thermal cycler for analyzing a biological material is provided that provides excellent temperature control in biological material reaction cycles. A thermal cycler for biological material is provided, including a heating/cooling unit capable of heating and/or cooling a biological material reaction region, and a control unit that controls an output power of the heating/cooling unit, in which the control unit controls the output power of the heating/cooling unit on the basis of a rate of temperature change of the biological material reaction region.

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Номер записи: 34

16-01-2020 дата публикации

Cell culture substrate, culture vessel, method for producing cell culture vessel, method for acquiring cells and method for culturing cells

Номер: US20200017815A1

Автор: Chie KOJIMA, Takeshi Kawano

Принадлежит: Nikon Corp, UNIVERSITY PUBLIC CORPORATION OSAKA

A cell culture substrate includes: a first layer that includes a first gel in which gold nanoparticles dispersed; and a second layer that includes a second gel in which the gold nanoparticles are not present or are present in a lower concentration in comparison with the first layer.

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Номер записи: 35

26-01-2017 дата публикации

Animal cell culture medium and culture container

Номер: US20170022477A1

Автор: Kyohei Ota, SATOSHI Tanaka

Принадлежит: Toyo Seikan Group Holdings Ltd

A culture medium for animal cells for enhancing a metabolism activity of the TCA cycle (tricarboxylic acid cycle) in respiration of the animal cells includes, as effective components, amino acids metabolized to succinyl CoA that is an element constituting the TCA cycle at a concentration of 2.3 mmol/L to 6.0 mmol/L.

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Номер записи: 36

25-01-2018 дата публикации

Visually determinable genetic testing

Номер: US20180023127A1

Автор: Hiroki Yamashita, Kazuhiko Kogoh, Shuichi OMURA

Принадлежит: Kurashiki Spinning Co Ltd

The purpose of the present invention is to provide a DNA detection technique by which testing can be simply and accurately performed at clinical sites or the like. The present invention provides a gene analysis method which employs, as a detection sample, a single strand nucleotide product that is amplified by ligation, and which can be performed visually in a more simple and rapid way.

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Номер записи: 37

24-01-2019 дата публикации

Photobioreactor systems and methods for producing biomass

Номер: US20190024029A1

Автор: Xiaoxi Wu

Принадлежит: Individual

Certain embodiments and aspects relate to a photobioreactor including encapsulating photobioreactor units (EPU) within which a liquid medium and a gas are encapsulated. The liquid medium comprises at least one species of phototrophic or mixotrophic organism therein. The gas comprises a high concentration of carbon dioxide. Certain methods of using the EPUs for algal biomass production use automated function units.

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Номер записи: 38

24-01-2019 дата публикации

Semi-dry bead beating method for microbial lysis and device for performing same

Номер: US20190024076A1

Автор: Beach Ryan, DRISCOLL Mark, Jarvie Thomas, Whynall Jeff

Принадлежит:

Disclosed are methods and devices for lysing cells to release extract genomic DNA (gDNA). The methods use a mixture of microscopic glass beads and cells (for example, spores) that form a semi-dry cake that clings to a larger metal ball and the sides of the tube during bead beating lysis, greatly improving the efficiency of the bead beating process. The devices produce a chaotic motion which ensures that sufficient force is generated to open the cells, and that the metal ball impacts are distributed across the interior surface of the container so that all of the cell mixture is subjected to sufficient impacts to break the cells. As a result, spores and other difficult-to-lyse microbes, can be opened in seconds. The method reduces the number of steps and hands-on time by rapidly opening difficult to lyse cells, while preserving the integrity of the DNA. 1. A device for shaking samples in a non-periodic motion and chaotic motion simultaneously , having a stroke length of from about 1 cm to about 2.5 cm , a stroke height from about 0.2 cm to about 1 cm , and a speed of from about 5 Hz to about 55 Hz.2. The device of claim 1 , wherein the device is configured to hold samples in one or more microtiter plates claim 1 , individual microcentrifuge tubes claim 1 , or other vessels suitable for low throughput or high-throughput analysis.3. The device of claim 2 , wherein the microtiter plate is selected from the group consisting of: 8 claim 2 , 16 claim 2 , 24 claim 2 , 46 claim 2 , 96 claim 2 , 384 or 1536 well plate.4. The device of claim 2 , wherein the number of microcentrifuge tubes that can be processed simultaneously ranges from 1 to 96.5. A method for lysing biologic cells in a sample to release DNA from the cells claim 2 , comprising the sequential steps of:(a) mixing a first aqueous or non-aqueous solution containing biologic cells with (i) a ball having a diameter of about 2 millimeters to about 10 millimeters, and (ii) beads having a diameter of about 20 ...

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Номер записи: 39

25-01-2018 дата публикации

Cell tracking correction method, cell tracking correction device, and storage medium which stores non-transitory computer-readable cell tracking correction program

Номер: US20180025211A1

Автор: Hideya ARAGAKI

Принадлежит: Olympus Corp

A processor of a cell tracking correction apparatus is configured to perform processes comprising: estimating a position of at least one cell in images acquired by time-lapse photography, and tracking the position of the cell; generating nearby area images of a nearby area including the cell from the images of photography time points of the time-lapse photography, based on the tracked position of the cell at each of the photography time points of the time-lapse photography; displaying the nearby area images on a display; accepting, via a user interface, an input of a correction amount for correcting the position of the cell with respect to one of the nearby area images displayed on the display unit; and correcting the tracked position of the cell corresponding to the nearby area image, in accordance with the correction amount.

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Номер записи: 40

23-01-2020 дата публикации

PHOTOBIOREACTOR

Номер: US20200024558A1

Автор: Levin Alexander

Принадлежит:

An invention proposes a photobioreactor with a cultivation chamber in the form of a shallow closed duct that is irradiated by the sun light. The duct is constructed from an inflatable sleeve from transparent polymer film; this shallow duct is sandwiched between a bank of frames with wire nettings from below and glass panes from above, which are secured on the shelves of the longitudinal sides of the frames. 1. A photobioreactor for cultivation of microalgae or cyanobacteria comprising: two parallel rows of posts and two parallel rows of inclined supporting angles installed on said posts; said posts with said supporting angles play a role of a supporting unit of said photobioreactor;said supporting unit comprises as well a bank of frames, which are installed on said supporting angels and abutted in- line; said frame are divided on two terminal frames and non- terminal frames; each said terminal frame comprises two longitudinal Z-profiles joined with two transverse strips; a sheet is installed on the bottom of each said terminal frame; each said non-terminal frame comprises two longitudinal Z-profiles joined with two transverse strips; a wire netting is installed on the bottom of each said non-terminal frame;a flat duct, which fulfills a role of a microalgae or cyanobacteria photo-cultivation chamber; said flat duct is arranged on said supporting unit and said flat duct comprises a longitudinal sleeve from transparent or translucent flexible polymer, which is positioned on said frames, wherein each terminal section of said longitudinal sleeve is provided with a port; abutted transparent or translucent glass panes are positioned on the upper shelves of said Z-profiles of said non-terminal frame with covering from above said longitudinal sleeve; said transparent or translucent glass panes are joined with said upper shelves of said Z-profiles by fastening members; terminal sections of said longitudinal sleeve comprise inlet and outlet ports for delivery of a gaseous ...

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Номер записи: 41

31-01-2019 дата публикации

Micro- and nanocontact printing with aminosilanes: patterning surfaces of microfluidic devices for multi- plexed bioassays

Номер: US20190030530A1

Автор: Amy Shen FRIED, Sebastien Georg Gabriel RICOULT

Принадлежит: Okinawa Institute of Science and Technology School Corp

It is an object of the present invention to achieve rapid surface patterning of biomolecules within microfluidic devices with high reproducibility. In this work, we present a new means of creating micro- and nano-patterns of aminosilanes within microfluidic devices via an aqueous based microcontact printing technique. To minimize the diffusion of molecules into the PDMS stamp, we use water as the inking solvent and enforce short incubation and contact times during the printing process to preserve the predefined resolution of patterned features. These patterns then serve as the building block to couple multiple biomolecules in solution onto a single surface for subsequent bioassays.

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Номер записи: 42

31-01-2019 дата публикации

Cell Culturing Method

Номер: US20190032006A1

Автор: Kinooka Masahiro, Nagamori Eiji

Принадлежит: OSAKA UNIVERSITY

Provided is a cell culturing method in which physical stress is reduced and oxygen can be supplied. The invention relates to a cell culturing method including; arranging cells in a dispersed state in a culture medium which is a plastic fluid; and introducing air bubbles into said culture medium. 1. A cell culturing method , comprising:arranging cells in a culture medium which is a plastic fluid; andintroducing air bubbles into said culture medium.2. The cell culturing method according to claim 1 , wherein the cells can be suspension-cultured.3. The cell culturing method according to claim 1 , wherein the cells are pluripotent stem cells or multipotent stem cells.4. The cell culturing method according to claim 1 , wherein the culture medium which is a plastic fluid is a culture medium that can maintain the cells in the culture medium without allowing them to sediment in a static state.5. The cell culturing method according to claim 1 , comprising culturing the cells using a cell culture vessel or a cell culture bag.6. The cell culturing method according to claim 1 , wherein the air bubbles are introduced intermittently or continuously.7. The cell culturing method according to claim 1 , wherein the air bubbles are microbubbles or submicrobubbles.8. The cell culturing method according to claim 1 , wherein the culture medium has a yield value of at least 0.03 Pa. The present disclosure relates to a cell culturing method using a plastic fluid.In regenerative medicine using human iPS (induced pluripotent stem) cells, utilization of techniques using animal cells such as antibody drug production using CHO (Chinese Hamster Ovary) cells has been promoted. Compared to two-dimensional plate culture, three-dimensional culture in which cells are cultured in a suspension state can increase the cell concentration per unit volume and thereby has advantages such as space saving and a reduction in the amount of the culture medium to be used.On the other hand, in culture in which a ...

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Номер записи: 43

04-02-2021 дата публикации

MULTIPLE INCUBATOR CELL CULTURE SYSTEM WITH ATMOSPHERIC REGULATION OPERATED BY AN INTEGRATED CONTROL SYSTEM

Номер: US20210032587A1

Автор: ERNDT Nicholas George, FETH Brian, LIM James, PHI Janette Trang, RUTHERFORD Becky

Принадлежит:

Embodiments of a cell culture incubator system provided herein have two or more individual incubators and atmospheric regulation system configured to regulate atmospheric conditions within the environmental chamber of each individual incubator. The atmospheric regulation system has a single integrated controller system that controls atmospheric regulation of each of the individual incubators independently of the one or more other individual incubators. Atmospheric conditions within each of the individual incubators include at least the oxygen level and the total gas pressure, which are regulated independently of each other. 1. A cell culture incubator system comprising:two or more individual incubators, wherein each individual incubator comprises an environmental chamber; andan atmospheric regulation system configured to regulate atmospheric conditions within the environmental chamber of each individual incubator, the atmospheric conditions comprising an oxygen level and a total gas pressure,wherein the atmospheric regulation system is configured to regulate the oxygen level and the total gas pressure within each incubator independently of each other, andwherein the atmospheric regulation system comprises a single integrated controller system configured to command atmospheric regulation of each of the individual incubators independently of the one or more other individual incubators.2. The cell culture incubator system of claim 1 , wherein the integrated controller system comprises:a single master controller operably linked to each of the individual incubators; andtwo or more subcontrollers, each subcontroller dedicated, respectively, to one of the two or more individual incubators,wherein the master controller is configured to deliver atmospheric condition set point commands to each of the two or more subcontrollers, and wherein each subcontroller regulates the atmospheric regulation system of the individual incubator to which it is dedicated, in accordance with ...

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Номер записи: 44

07-02-2019 дата публикации

DEVICE AND METHOD FOR SAMPLE ANALYSIS

Номер: US20190040344A1

Автор: Celedon Alfredo A., Pettit John W.

Принадлежит:

The present invention describes a device used to process a sample and detect the presence or absence of certain molecules in the sample. The device includes a pouch having two or more compartments that may be created by sealing two or more films. In a preferred embodiment, the pouch is made by a single sheet of film that has been folded onto itself. In another preferred embodiment, the pouch is made by sealing two films. In other aspects, this invention describes methods to process a sample. 2. The pouch of claim 1 , wherein the pouch is made by heat-sealing one or two films.3. The pouch of claim 1 , wherein at least one of the flow channels has a substantially U-shape claim 1 , and wherein the single device comprises a clamp that exerts pressure on at least two surfaces of the flow channel.4. The pouch of claim 1 , wherein the pouch comprises at least five compartments and at least five flow channels claim 1 , and wherein the single device is associated with the pouch and is capable of closing the at least five flow channels at the same time.6. The pouch of wherein the first and second fluidic connectors are releasable fluidic connectors.7. The pouch of wherein the first and second fluidic connectors are hollow needles.8. The pouch of wherein the first and second fluidic connectors are Luer taper connectors.9. The pouch of claim 5 , wherein the pouch is made by heat-sealing one or two films.10. The pouch of claim 7 , wherein the needles are substantially parallel to each other.11. The pouch of claim 7 , wherein the needles are positioned to be from about 1 to about 30 millimeters apart.12. The pouch of claim 5 , wherein the first and second fluid connections are configured such that they are capable of being closed with a single device that exerts pressure on such fluid connections.13. The pouch of claim 12 , wherein the first and second fluid connections comprise substantially U-shaped channels.15. The pouch of claim 14 , wherein the container comprises a cap that ...

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Номер записи: 45

18-02-2021 дата публикации

MICRO- AND NANOCONTACT PRINTING WITH AMINOSILANES: PATTERNING SURFACES OF MICROFLUIDIC DEVICES FOR MULTI- PLEXED BIOASSAYS

Номер: US20210046476A1

Автор: FRIED Amy Shen, RICOULT Sebastien Georg Gabriel

Принадлежит:

It is an object of the present invention to achieve rapid surface patterning of biomolecules within microfluidic devices with high reproducibility. In this work, we present a new means of creating micro- and nano-patterns of aminosilanes within microfluidic devices via an aqueous based microcontact printing technique. To minimize the diffusion of molecules into the PDMS stamp, we use water as the inking solvent and enforce short incubation and contact times during the printing process to preserve the pre-defined resolution of patterned features. These patterns then serve as the building block to couple multiple biomolecules in solution onto a single surface for subsequent bioassays. 1. A method of micro or nano patterning of biomolecules in a multiplex format comprising the steps ofcovering a silicone wafer (Si Wafer) which has nanoholes with PDMS;separating the PDMS from the Si Wafer;covering the PDMS with a photo-sensitive polymer;exposing the photo-sensitive polymer to light;separating the photo-sensitive polymer replica from the PDMS;contacting the photo-sensitive polymer replica which was plasma activated with a planar PDMS stamp which was incubated with silane;separating the flat PDMS to lift-off silane in the contact areas;printing the flat PDMS with nanoholes of silane onto a glass slide;separating the flat PDMS from the glass slide;incubating the patterned silane with an aqueous PEG silane to block the unpatterned surface; and thenincubating the patterned/blocked APTES silane with desired biomolecule.2. The method of wherein the biomolecule is selected from the group consisting of proteins claim 1 , peptides claim 1 , antibodies claim 1 , nucleic acids claim 1 , carbohydrates and lipids.3. The method of wherein the diameter of nanoholes is patterned from 10 nm to 1000 nm. This application is the Divisional of U.S. patent application Ser. No.: 16,073,791, filed on Jul. 29, 2018, which is the National Stage Entry of PCT/JP2017/003621, filed Feb. 1, 2017, ...

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Номер записи: 46

25-02-2021 дата публикации

Motion controlling mechanism, liquid discharging nozzle, microdroplet generating device and method, liquid driving mechanism and method, microdroplet generating method, and surface processing method of liquid discharging nozzle

Номер: US20210053046A1

Автор: Guang-Ji Sheng

Принадлежит: Sniper Beijing Medical Technologies Co Ltd

Disclosed is a liquid discharging nozzle, including a needle stem having a hollow chamber and an outlet end located at one end of the needle stem, an angle between a normal line of an end surface of the outlet end of the liquid discharging nozzle and an extension direction of the needle stem is equal to or smaller than 90°. Further disclosed are a motion controlling mechanism, a microdroplet generating device and method, a liquid driving mechanism and method, a microdroplet generating method, and a surface processing method of a liquid discharging nozzle.

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Номер записи: 47

25-02-2021 дата публикации

Improved cartridge for use in in-vitro diagnostics and method of use thereof

Номер: US20210053051A1

Автор: Amit Schnell, Ari Tadmor, Asaf LIPSHITZ, Dalibor Hodko, Eran ZAHAVI, Lutz Weber, Mario Schmitt, Paul D. Swanson, Vladimir Hurgin

Принадлежит: Ador Diagnostics Srl

A cartridge for use in in-vitro diagnostics, the cartridge including a cartridge housing, a cartridge element, disposed within the cartridge housing and defining a plurality of operational volumes, at least some of the plurality of operational volumes being mutually linearly aligned, a fluid solution transporter operative to transfer fluid solutions from at least one of the plurality of operational volumes to at least another of the plurality of operational volumes, the fluid solution transporter including a linearly displaceable transport element operative to sequentially communicate with interiors of the at least some of the plurality of operational volumes and a venter, including a linearly displaceable venting element, operative in coordination with the fluid solution transporter to vent at least one of the plurality of operational volumes.

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Номер записи: 48

14-02-2019 дата публикации

CELL CULTURE VESSEL AND JIG FOR FIXING CELL CULTURE VESSEL

Номер: US20190048302A1

Автор: Morimura Takashi, Suzuki Ikumi, Yoshida Takao

Принадлежит: FUKOKU CO., LTD.

The present invention provides a cell culture vessel and a jig for fixing the cell culture vessel in position. More specifically, the cell culture vessel of the present invention is provided with a vessel portion having an annular sealed culture space when viewed from overhead and a port portion connecting the inner portion and outer portion of the culture space. The vessel portion is provided with a first vessel wall serving as the upper side during culturing and a second vessel wall serving as the lower side during culturing. Preferably at least the first vessel wall has flexibility. 1. A cell culture vessel , comprising:a vessel portion having an annular sealed culture space when viewed from overhead; anda port portion connecting the inner portion and outer portion of the culture space; wherein,the vessel portion is provided with a first vessel wall serving as the upper side during culturing and a second vessel wall serving as the lower side during culturing.2. The cell culture vessel according to claim 1 , wherein the first vessel wall has flexibility.3. The cell culture vessel according to claim 1 , wherein both the first vessel wall and the second vessel wall have flexibility.4. The cell culture vessel according to claim 1 , wherein the first vessel wall has flexibility and the second vessel wall has a shape-retaining property.5. The cell culture vessel according to claim 4 , provided with an annular outer peripheral wall rising from the outer periphery of the second vessel wall and an annular inner peripheral wall rising from the inner periphery of the second vessel wall.6. The cell culture vessel according to claim 1 , wherein both the first vessel wall and the second vessel wall have a shape-retaining property.7. The cell culture vessel according to claim 2 , wherein the inner surface of the first vessel wall and the inner surface of the second vessel wall are substantially adhered to each other when contents containing a gas are discharged from inside the ...

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Номер записи: 49

14-02-2019 дата публикации

METHOD OF EVALUATING QUALITY OF miRNA DERIVED FROM BODY FLUID

Номер: US20190048408A1

Автор: Kazue NATORI, Satoko Kozono, Satoshi Kondou

Принадлежит: TORAY INDUSTRIES INC

A method of evaluating quality of miRNA derived from a body fluid sample includes a measuring step; comparing a measured value(s) of the abundance(s) of the one or more reference miRNAs in a body fluid sample or a representative value thereof to a measured value(s) of the abundance(s) of the one or more reference miRNAs in a standard body fluid sample or a representative value thereof, to obtain a difference(s) or a ratio(s) of the measured value(s) of the abundance(s) of the one or more reference miRNAs or the representative value thereof between the body fluid sample and the standard body fluid sample; and judging the quality of the miRNA derived from the body fluid sample based on the difference(s) or the ratio(s) of the measured value(s) of the abundance(s) of the one or more reference miRNAs or the representative value thereof obtained in the comparing step.

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Номер записи: 50

25-02-2021 дата публикации

SWITCHING VALVE AND SUCTION-DISCHARGE DEVICE INCLUDING THE SAME

Номер: US20210054329A1

Автор: Munakata Teruyoshi, Tanioka Atsuyoshi, YAMASAKI Yukito

Принадлежит:

A switching valve includes a rotor having a pair of rollers rotatably mounted on both ends thereof, a rotor drive unit rotationally driving the rotor, a pair of pressing members, each being provided at a position where each of the pair of pressing members cooperates with each of the pair of rollers outside a revolution orbit of each of the pair of rollers revolving by rotation of the rotor, and a pair of tubes, each being disposed between the revolution orbit of each of the pair of rollers and each of the pair of pressing members. A rotation center axis of the rotor is disposed on a straight line connecting centers of rotation of the pair of rollers, and each pressing member has the pair of pressing areas symmetrical with respect to a straight line passing through the center of rotation of the rotor and extending a vertical direction. 1. A switching valve comprising;a rotor that includes a pair of rollers rotatably mounted on both ends thereof;a rotor drive unit that rotationally drives the rotor;a pair of pressing members, each of the pair of pressing members being provided at a position where each of the pair of pressing members cooperates with each of the pair of rollers outside a revolution orbit of each of the pair of rollers revolving by rotation of the rotor; anda pair of tubes, each of the pair of tubes being disposed between the revolution orbit of each of the pair of rollers and each of the pair of pressing members,wherein a gap between each of the pair of pressing members and the revolution orbit of each of the pair of rollers is narrow, and a pair of pressing areas where the pair of rollers and the pair of pressing members cooperate with each other to press and block one or both of the pair of tubes is formed by revolution of the pair of rollers,the pair of rollers is rotationally movable among a position where the pair of tubes is both blocked, a position where only one of the pair of tubes is blocked, and a position where flows in the pair of tubes are ...

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Номер записи: 51

23-02-2017 дата публикации

Culture container

Номер: US20170051241A1

Автор: Atsushi Onishi, Hiromi Yamamoto, Naoko Obi, Tetsuya Nakayama, Yoichi Yamaguchi

Принадлежит: Nissha Printing Co Ltd

A culture container includes a container main body and a filmlike member. The container main body has a recessed part and a bottom part 17 in which a plurality of microwells for containing a cell-culture solution is formed. The filmlike member is a member that is disposed above the plurality of microwells inside the recessed part and limits the movement of each spheroid that has grown inside its corresponding microwell such that the spheroid does not separate from the corresponding microwell. Thus, spheroids can be stably formed in the culture container.

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Номер записи: 52

22-02-2018 дата публикации

Methods of amplifying nucleic acids and compositions and kits for practicing the same

Номер: US20180051330A1

Автор: Eileen Snowden, Frances Poyen Tong, Nicholas Herrmann, Richard Lee Kelley, Terry J. Amiss

Принадлежит: Becton Dickinson and Co

Provided are methods of amplifying nucleic acids. The methods include combining a nucleic acid sample, a known amount of one or more competitive internal standard nucleic acids, and one or more amplification primers adapted to amplify one or more nucleic acids of interest present in the nucleic acid sample and the one or more competitive internal standard nucleic acids. The nucleic acid sample, competitive internal standard nucleic acids, and amplification primers are combined in a reaction mixture under conditions sufficient to amplify the one or more nucleic acids of interest and the one or more competitive internal standard nucleic acids. Aspects of the present disclosure further include compositions and kits that find use in practicing embodiments of the methods.

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Номер записи: 53

21-02-2019 дата публикации

LOW SHEAR, LOW VELOCITY DIFFERENTIAL, IMPELLER HAVING A PROGRESSIVELY TAPERED HUB VOLUME WITH PERIODS FORMED INTO A BOTTOM SURFACE, SYSTEMS AND METHODS FOR SUSPENSION CELL CULTURING

Номер: US20190054432A1

Автор: BACHELLIER Carl R.

Принадлежит:

Disclosed is an impeller for mixing a fluid wherein the impeller is fashioned so as to substantially reduce areas of fluid acceleration and deceleration around a hub region of the impeller during fluid mixing. Additionally, a bioreactor system having microcarriers, kit of parts and cell culturing method are also provided. The impeller has a generally circularly shaped hub having a downwardly directed progressively tapered volume which has plurality of periods formed therein corresponding to the number of increasingly arced blades. Each of the increasingly arced blades flare outwards to a distal end region of the impeller and have an increasing radius to said arc towards a peripheral edge region of said hub. The flare to the increasingly arced blades defines a larger circumference than that of said hub, thereby imparting a generally frusto-conical shape to the impeller. A method mixing a fluid using the impeller disclosed herein is also disclosed. 1) An impeller couplable to a rotatable drive by way of a rotatable drive attachment region coupled to a generally circularly shaped hub , said generally circularly shaped hub including a downwardly directed progressively tapered volume having coupled thereto a proximal end region of one or more arced blades arranged along a periodic pattern formed into said downwardly directed progressively tapered volume wherein the number of periods corresponds to the number of blades;each of said one or more arced blades having an increasing radius to said arc towards a peripheral edge region of said hub;said proximal end region of said one or more increasing radius arced blades extending from said peripheral edge region of said hub along said downwardly directed progressively tapered volume to a predetermined point inward of said peripheral edge region;said one or more arced blades further being flared as extending from said progressively tapered volume such that a distal end region of each of said one or more increasing radius arced ...

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Номер записи: 54

28-02-2019 дата публикации

SAMPLE CONTAINER FOR STABILIZING AND ALIGNING EXCISED BIOLOGICAL TISSUE SAMPLES FOR EX VIVO ANALYSIS

Номер: US20190062681A1

Автор: FAN Chao, Jackson James, MUNRO Elizabeth A., Rempel David

Принадлежит: Perimeter Medical Imaging, Inc.

Embodiments described herein relate to an apparatus for positioning and securing an excised biological tissue specimen for imaging and analysis. In some embodiments, an apparatus includes a sample bag defining an inner volume configured to receive a biological tissue sample, and a sealing member coupled to the sample bag. An imaging window is disposed and configured to be placed in contact with at least a portion of the biological tissue sample, and a positioning member is coupled to the imaging window and is configured to be disposed against the sealing member to substantially seal the inner volume. The positioning member includes a vacuum port disposed and configured to be aligned with a vacuum source to withdraw air from the inner volume of the sample bag. 1. An apparatus for holding a biological tissue sample in a substantially fixed position , the apparatus comprising:a sample bag defining an inner volume configured to receive the biological tissue sample;a sealing member coupled to the sample bag;an imaging window substantially spanning the opening of the sample bag and configured to be placed in contact with at least a portion of the biological tissue sample; anda positioning member coupled to the imaging window and configured to be disposed against the sealing member, the positioning member including a vacuum port disposed and configured to be aligned with a vacuum source to withdraw air from the inner volume of the sample bag.2. The apparatus of claim 1 , wherein the inner volume is substantially sealed when the positioning member is disposed against the sealing member.3. The apparatus of claim 1 , further comprising:a receiving member having a channel to which a portion of the positioning member is configured to be disposed, the channel defined at least in part by a first wall spaced a distance from a second wall.4. The apparatus of claim 3 , further comprising:a locking mechanism configured to retain the portion of the positioning member disposed within ...

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Номер записи: 55

28-02-2019 дата публикации

PROCESS FOR OPTIMIZING THE OPERATION OF A PLUG-FLOW FERMENTER FOR THE ANAEROBIC FERMENTATION OF ORGANIC WASTES

Номер: US20190062682A1

Автор: EUGSTER Marc, SCHATZ Adrian

Принадлежит: HITACHI ZOSEN INOVA AG

A process for optimizing the operation of a plug-flow fermenter for the anaerobic fermentation of organic wastes, wherein the plug-flow fermenter comprises a horizontally oriented fermenter tank and a stirrer, which stirrer comprises a stirrer shaft which traverses the interior of the fermenter tank in an axial manner and multiple paddles which are arranged on the stirrer shaft and protrude radially and also a drive, and the fermentation material is moved in the fermenter tank by means of the stirrer. 1. A process for optimizing the operation of a plug-flow fermenter for the anaerobic fermentation of organic wastes , wherein the plug-flow fermenter comprises a horizontally oriented fermenter tank and a stirrer , which stirrer comprises a stirrer shaft which traverses the interior of the fermenter tank in an axial manner and multiple paddles which are arranged on the stirrer shaft and protrude radially and also a drive , and the fermentation material is moved in the fermenter tank by means of the stirrer , whereina) at least one parameter characteristic of the particular operating state of the plug-flow fermenter is measured,{'sub': actual', 'nominal, 'b) the particular measurement value Aobtained in a) is compared with a predefined nominal value A, and'}{'sub': actual', 'nominal, 'c) depending on the deviation of the measurement value Afrom the nominal value A, the rotational speed of the stirrer shaft, the rotational direction of the stirrer shaft and/or the dry-substance portion of the fermentation material is adjusted.'}2. The process as claimed in claim 1 , wherein the adjustment of the dry-substance portion of the fermentation material is done via the amount of wetting agent introduced into the fermenter tank.3. The process as claimed in claim 1 , wherein the rotational speed of the stirrer shaft claim 1 , the rotational direction of the stirrer shaft and/or the dry-substance portion of the fermentation material is regulated in step c) depending on the ...

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Номер записи: 56

28-02-2019 дата публикации

Reaction container and biochemical analysis method

Номер: US20190062821A1

Автор: Akihiro Hoshino, Masahiro MATSUKAWA, Yoichi Makino

Принадлежит: Toppan Printing Co Ltd

A reaction container including a transparent base having a first surface having at least one region where recessed portions are formed and recessed from the first surface, and a cover member positioned such that the cover member forms a gap from the first surface inside the region and is welded to the transparent base outside the region. The cover member absorbs infrared light and transmits light having a wavelength within a range of visible light.

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Номер записи: 57

29-05-2014 дата публикации

Apparatus and method for cultivating microalgae using effluent from sludge treatment

Номер: US20140144839A1

Автор: Gyobeom Kim, Jae Shik Chung, Kyu Won Seo, Seok Won Hong, Yong Su Choi

Принадлежит: Korea Advanced Institute of Science and Technology KAIST

Disclosed herein are an apparatus and method for cultivating microalgae using effluent from sludge treatment. The apparatus includes an advanced sewage treatment apparatus, a sludge treatment apparatus and a microalgae cultivation apparatus, the sludge treatment apparatus including: a first aerobic reactor which is operated under aerobic conditions and serves to ferment sludge; a second aerobic reactor which is operated in a state in which air is injected in an amount larger than that in the first aerobic reactor, and serves to increase the fermentation activity of microorganisms in the sludge and degrade the sludge; a membrane bio-reactor (MBR) which serves to receive effluent from the second aerobic reactor and biologically remove high-concentration organic matter from the effluent by the action of aerobic microorganisms while removing total suspended solids using a membrane, wherein the effluent discharged from the MBR reactor is supplied to the microalgae cultivation apparatus.

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Номер записи: 58

11-03-2021 дата публикации

CELL CULTURE VESSEL

Номер: US20210071121A1

Автор: KANAMORI Toshiyuki, Matsumoto Junichi, Nishi Norio, SUMARU Kimio, Suzuki Masami

Принадлежит:

A cell culture vessel for use with a laser processing machine, including: a layer containing an ingredient that generates heat upon laser irradiation; the layer kills specific cells from among a group of cells cultured on a first surface of the layer when a second surface of the layer is irradiated with laser light having an output of 5W or less at a wavelength of 380 nm or greater and a relative movement between a second area on the second surface where the laser light is applied and the layer is at a rate of 2000 mm/sec or less such that the second area absorbs energy of the laser light to generate heat that kills the specific cells that are present on a first area of the first surface and the laser light does not instantly kill the specific cells on the first area upon irradiation with the laser light. 1. A cell culture vessel for use with a laser processing machine , the cell culture vessel comprising:a to-be-irradiated layer containing an ingredient configured to generate heat upon laser irradiation;wherein the to-be-irradiated layer is configured to kill specific cells from among a group of cells cultured on a first surface of the to-be-irradiated layer when a second surface of the to-be-irradiated layer is irradiated with laser light having an output of 5W or less at a wavelength of 380 nm or greater and a relative movement between a second area on the second surface of the to-be-irradiated layer where the laser light is applied and the to-be-irradiated layer is at a rate of 2000 mm/sec or less such that the second area of the to-be-irradiated layer irradiated with the laser light absorbs energy of the laser light to generate heat that kills the specific cells that are present on a first area of the first surface opposed to the second area on the second surface of the to-be-irradiated layer and the laser light does not instantly kill the specific cells on the first area upon irradiation with the laser light.2. The cell culture vessel according to claim 1 , ...

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Номер записи: 59

24-03-2022 дата публикации

Method and system for cell operations using the internet of things

Номер: US20220089999A1

Автор: Cannon Howard, Farb Thomas Forest

Принадлежит:

A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation. 151.-. (canceled)52. A cell laboratory data collection hub comprising: a controller configured to receive data from a plurality of sensors including at least one sensor for sensing ambient conditions in a laboratory , at least one sensor for sensing the identity of a cell operation supply material held in a container and used in a cell operation in an instrument , at least one sensor for sensing a state of container of the cell operation supply material , a controller receiving data from the plurality of sensors and cell data relating to the status of cells being operated on; a database for archiving the cell data and the data from the plurality of sensors; and wherein the controller upon determining a difference between the status of cells in a current cell operation from the archived status of cells of a past operation , provides a user with a comparison of data from the plurality of sensors from the past operation and the current operation to account for the difference.53. The data collection hub according to claim 52 , wherein the cell operation is cell culturing and the status of cells comprises at least one of confluence and cell count.54. The data collection hub according to claim 52 , wherein the conditions related to the performance of operations on cells includes alerts.55. The data collection hub according to claim 52 , wherein the conditions related to the performance of operations on cells includes alerts to people.56. The data collection hub according to claim 52 , wherein the conditions related to the performance of operations on cells includes ...

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Номер записи: 60

05-03-2020 дата публикации

Manufacturing Method and Device for Three-Dimensional Engineered Tissue

Номер: US20200071662A1

Автор: ONO Jiro

Принадлежит:

A method for fabricating a cellular structure is disclosed. The method includes feeding cell aggregates onto a plurality of thread or needle shaped members in each of a plurality of supports. Each member forms net or mesh shaped spaces in the respective support. The method also includes layering the supports with the cell aggregates fed therein, culturing the cell aggregates to fuse, and removing the plurality of thread or needle shaped members from the fused cellular structure. 1. A support for fabricating a three-dimensional cellular structure , the support comprising:a first frame placed in an x-direction, a first frame placed in a y-direction, a second frame placed in the x-direction, and a second frame placed in the y-direction,a plurality of string members placed in the x-direction, wherein each string member within the plurality of string members placed in the x-direction is connected to the first frame and/or the second frame placed in the y-direction, and wherein a gap distance (Px) between each string member within the plurality of string members placed in the x-direction is between about 0.01 mm and about 3 mm;a plurality of string members placed in the y-direction, wherein each string member within the plurality of string members placed in the y-direction is connected to the first frame and/or the second frame placed in the x-direction, and wherein a gap distance (Py) between each string member within the plurality of string members placed in the y-direction is between about 0.01 mm and about 3 mm;wherein the plurality of string members placed in the x-direction and the plurality of string members placed in the y-direction are perpendicular to each other in a same plane and form a plurality of rectangular net shaped or matrix shaped spaces in the same plane inside each support of the one or more support;wherein the plurality of net shaped or matrix shaped spaces defines a plurality of physical boundaries capable of holding cell aggregates; andwherein the ...

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Номер записи: 61

18-03-2021 дата публикации

Instrument and method for energizing molecules in charged droplets

Номер: US20210078004A1

Автор: Daniel R. Fuller, Daniel W. WOODALL, David E. Clemmer, David H. Russell, Evan R. Williams, Tarik J. EL-BABA

Принадлежит: Indiana University Research And Technology Corp

An instrument for energizing molecules contained in a sample solution may include a droplet generator configured to generate droplets of the sample solution. The droplet generator illustratively has an elongated nozzle defining an orifice at one end thereof from which the droplets exit the droplet generator, and the orifice illustratively defines a first longitudinal axis centrally therethrough. A molecule energizing source is configured to produce a molecule energizing field, and is positioned relative to the nozzle orifice such that the molecule energizing field extends into at least some of the generated droplets along a direction non-parallel with the first longitudinal axis. The molecule energizing field illustratively carries energy which heats at least one of the generated droplets sufficiently to induce structural changes in at least one molecule contained in the at least one of the generated droplets.

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Номер записи: 62

18-03-2021 дата публикации

Laser processing machine

Номер: US20210079324A1

Автор: Junichi Matsumoto, Kimio Sumaru, Masami Suzuki, Norio Nishi, Toshiyuki Kanamori

Принадлежит: Kataoka Corp, National Institute of Advanced Industrial Science and Technology AIST

A laser processing machine for killing specific cells from a group of cells on a surface of a layer containing an ingredient capable of absorbing laser light, the laser processing machine being configured to: control a laser light source to output laser light at 5 W or less and at a wavelength of 380 nm or greater such that the laser light source is applied to a second area on a second surface of the layer opposed to the first surface; and control an actuator to provide a relative movement between the second area where the laser light is applied and the layer at a rate of 2000 mm/sec or less such that the irradiated second area absorbs energy to generate heat that kills unwanted cells on a first area of the first surface and the laser light does not instantly kill the specific cells on the first area upon irradiation.

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Номер записи: 63

18-03-2021 дата публикации

GAS FERMENTATION USING MULTIPLE-PASS TRICKLE BED REACTORS

Номер: US20210079326A1

Автор: Bennett Albert, Orr Jonathan N.

Принадлежит:

The subject matter of this application is using novel biological reactors for the fermentation of gases into liquid products. More specifically, the subject matter relates to the use of a Multiple-Pass Trickle Bed Reactor (MP-TBR) for the anaerobic or aerobic and biological fermentation of gases generated from industrial processes and/or from the gasification of biomass and other organic carbon sources. The products may include, but are not limited to, ethanol and other valuable chemicals. 1. A device used to carry out biological fermentation processes in a production facility comprising:a multiple-pass trickle bed reactor to provide anaerobic conditions for production of alcohols, acids and/or metabolic by-products, based on the biological fermentation of gases generated by industrial processes or the gasification of organic materials.2. The device of claim 1 , wherein the multiple-pass trickle bed reactor comprises a biological reactor having a single vessel claim 1 , or multiple vessels configured in series.3. The device of claim 1 , wherein the multiple-pass trickle bed reactor comprises operating at a pressure of 1 psig.4. The device of claim 1 , wherein the multiple-pass trickle bed reactor comprises:a single gas inlet end to inject fermentable gases, anda single gas outlet end to exhaust unused fermentable gases and gaseous fermentation by-products from the multiple-pass trickle bed reactor.5. The device of claim 1 , wherein the multiple-pass trickle bed reactor comprises a single vessel that contains a single internal subsection or a single vessel that contains multiple internal subsections that are configured in series.6. The device of claim 1 , wherein the multiple-pass trickle bed reactor comprises a reactor with multiple vessels configured in series claim 1 , wherein each vessel can contain a single internal subsection or each vessel can contain multiple internal subsections that are configured in series.7. The device of claim 1 , wherein the multiple- ...

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Номер записи: 64

12-03-2020 дата публикации

Microchip

Номер: US20200078783A1

Автор: Hidetoshi Watanabe

Принадлежит: Eiken Chemical Co Ltd

A microchip 1 equipped with a chip main body 2 in which a reaction space 3 depressurized with respect to an atmospheric pressure is formed, in which the chip main body 2 has a two-layer structure of a first plate-shaped part 7 having gas impermeability, and a second plate-shaped part 8 having a self-sealing property, which is laminated on one surface of the first plate-shaped part 7 , and the reaction space 3 is formed between the first plate-shaped part 7 and the second plate-shaped part 8.

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Номер записи: 65

31-03-2022 дата публикации

Method and system for cell operations using the internet of things

Номер: US20220098541A1

Автор: Cannon Howard, Farb Thomas Forest

Принадлежит:

A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation. 151.-. (canceled)52: A cell laboratory data collection method comprising the steps of: providing a plurality of sensors including at least one sensor for sensing ambient conditions in a laboratory , and at least one sensor for sensing the identity of a cell operation supply material held in a container and used in a cell operation in an instrument , configuring a controller to receive data from the plurality of sensors and cell data relating to the status of cells being operated on; archiving the cell data and the data from the plurality of sensors in a database; and when there is a difference between the status of cells in a current cell operation differs from the status of cells of a past operation that has been archived , providing to the user a comparison of data from the plurality of sensors from the past operation and the current operation to account for the difference.5326: The data collection method according to claim , wherein the cell operation is cell culturing and the status of cells comprises at least one of confluence and cell count.5426: The data collection method according to claim , wherein the conditions related to the performance of operations on cells includes alerts.5526: The data collection method according to claim , wherein the conditions related to the performance of operations on cells includes alerts to people.5626: The data collection method according to claim , wherein the conditions related to the performance of operations on cells includes alerts to computers.5726: The data collection method according to claim , wherein the controller ...

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Номер записи: 66

25-03-2021 дата публикации

Fermentation tank with an inlet for injecting carbon dioxide

Номер: US20210087523A1

Автор: Subir Kumar Nandy

Принадлежит: Unibio AS

A fermentation tank is shown with an inlet for injecting at least one gaseous substrate into the tank, wherein the at least one gaseous substrate includes carbon dioxide (CO2). One or more sensors may be included. Such sensors may be selected for determining gasses (such as CO2, methane, oxygen, etc.), nutrition, minerals, and/or pH. Active or static mixing devices may be included. The tank may be used in a method for improving biomass production and/or growth rate of a microorganism in a fermentation process such as shown and disclosed.

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Номер записи: 67

29-03-2018 дата публикации

CELL CULTURE APPARATUS AND CELL CULTURE METHOD

Номер: US20180085726A1

Автор: KANAMORI Toshiyuki, SATOH Taku, SHIN Kazumi, SUGIURA Shinji

Принадлежит:

A cell culture apparatus, includes a connected culture container including n number of units disposed in parallel along a second direction that is a different direction from a first direction where each of the n number of the units is constituted of m number of culture chambers and one or more communication-channels, the m number of the culture chambers each having a cell-holding portion that holds seeded cells, the m number of the culture chambers storing liquid culture media, the m number of the culture chambers being disposed in parallel along the first direction, the communication-channels communicating the m number of the culture chambers with each other; and a plurality of pneumatic pipes communicating same-row-disposed culture chambers that are disposed on a same row along the second direction with each other in the connected culture container. 1. A cell culture apparatus , comprising:a connected culture container comprising n number of units disposed in parallel along a second direction that is a different direction from a first direction where each of the n number of the units is constituted of m number of culture chambers and one or more communication-channels, the m number of the culture chambers each having a cell-holding portion that holds seeded cells, the m number of the culture chambers storing liquid culture media, the m number of the culture chambers being disposed in parallel along the first direction, the communication-channels communicating the m number of the culture chambers with each other; anda plurality of pneumatic pipes communicating same-row-disposed culture chambers that are disposed on a same row along the second direction with each other in the connected culture container, the pneumatic pipes being configured to pressurize the same-row-disposed culture chambers at the same time or open the same-row-disposed culture chambers to atmospheric pressure at the same time, the pneumatic pipes being configured to send the liquid culture media ...

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Номер записи: 68

29-03-2018 дата публикации

CELL THERAPY WITH POLARIZED MACROPHAGES FOR TISSUE REGENERATION

Номер: US20180087031A1

Автор: CASTILLO GARCÍA Adrián, DOMÍNGUEZ SÁNCHEZ Rubén, GARCÍA DE LA RIVA MESTRE Pablo, GINESTA BUCH Xavier, HOTTER CORRIPIO María Georgina, MARTÍN CORDERO Jorge Vicente, RODRÌGUEZ GARCIA Anna, SÁNCHEZ MORENO Jaime, SOLA MARTINEZ Ana Maria

Принадлежит:

The invention provides an in vitro method for inducing macrophage polarization to an M2 phenotype useful for tissue repair. The method described in the present invention comprises the in vitro exposure of macrophages to repeated series of hypoxia-reoxygenation. Activated M2 macrophages obtained by this method overexpress molecules important for tissue remodeling and amelioration of inflammation, such as NGAL and anti-inflammatory cytokines (IL-10). Thus, M2 macrophages obtained by this method are useful as cell therapy for tissue regeneration. The invention also provides pharmaceutical compositions and kits comprising the M2 macrophages obtained by the described method. The invention further refers to a device for inducing hypoxia and re-oxygenation conditions on isolated macrophages according to the described method. 1. A method for obtaining macrophages polarized to an M2 phenotype that comprises:a. subjecting isolated macrophages to 2, 3 or 4 series of hypoxia-reoxygenation, andb. recovering the macrophages obtained after step (a).2. The method according to claim 1 , wherein the step (a) consists of 4 series of hypoxia-reoxygenation.3. The method according to claim 1 , wherein each series of hypoxia-reoxygenation comprises between 2 and 5 minutes of hypoxia followed by at least 45 seconds of reoxygenation.4. The method according to claim 1 , which comprises an additional step (a′) claim 1 , between the steps (a) and (b) claim 1 , comprising subjecting the macrophages obtained after step (a) to a final reoxygenation step.5. The method according to claim 4 , wherein the final reoxygenation step is performed during no more than 1 hour and 30 minutes.6. The method according to claim 1 , wherein the macrophages of step (a) are monocytes.7. An M2 macrophage or a population of M2 macrophages obtainable by the method according to claim 1 , wherein said M2 macrophage overexpresses at least NGAL and IL10.8. A pharmaceutical composition comprising the M2 macrophage or the ...

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Номер записи: 69

21-03-2019 дата публикации

EVALUATION DEVICE, OBSERVATION DEVICE, AND PROGRAM

Номер: US20190087956A1

Автор: Furue Miho, KII Hiroaki, KIYOTA Yasujiro, SUGA Mika, Uozumi Takayuki

Принадлежит:

An evaluation device includes a state determination unit which is configured to determine a state of cells which are an observation target under non-standard conditions on the basis of information acquired from an image of cells under standard conditions. 1. An evaluation device comprising:a state determination unit which is configured to determine a state of cells which are observation targets under non-standard conditions on the basis of information acquired from an image of cells under standard conditions.2. The evaluation device according to claim 1 , wherein the information acquired from an image of cells under the standard conditions is information about a marker which is used to evaluate information on the cells.3. The evaluation device according to claim 1 , wherein the state determination unit is configured to determine the state of cells which are observation targets under the non-standard conditions on the basis of information on a marker which is used to evaluate the information on cells acquired from an image of cells under the standard conditions and information about a marker which is used to evaluate information on cells which are observation targets under the non-standard conditions.4. The evaluation device according to claim 1 , wherein the information on cells under the standard conditions is information acquired from an image with respect to a cell differentiation process.5. The evaluation device according to claim 1 , wherein the information on cells under the standard conditions is information acquired from images with respect to cell differentiation captured at more than one different times.6. The evaluation device according to claim 1 , wherein the information on cells under the standard conditions is information on cells under a condition in which a substance has been added to cells claim 1 , and the information on cells under the standard conditions is information under a condition in which no substance is added to cells.7. The evaluation ...

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Номер записи: 70

12-04-2018 дата публикации

CELL ENCAPSULATION LOADING DEVICE

Номер: US20180099129A1

Автор: Bell Audrey, Evans Stephen

Принадлежит: JANSSEN BIOTECH, INC.

The present invention provides a loading fixture for loading cells, cell clusters, and media to an immune-isolation device. The loading fixture permits the loading of cells into the immune-isolation device without manual manipulation of the immune-isolation device, until the loading device is opened at the point of use, thereby minimizing the risk of contamination of the immune-isolation device. 1. A method of preparing immune-isolation devices , comprising: a base having a base bottom surface and a base wall extending from the base bottom surface to form a cavity,', 'an inlet conduit positioned on the base and extending there through into the cavity, and', 'a disk lid removably attachable to the base to seal the cavity;, 'providing a loading system comprisingcoupling an immune-isolation device comprising an inlet tube attached to a pouch, to the inlet conduit positioned on the base within the cavity;sealing the cavity with the disk lid;filling the pouch of the immune-isolation device with cells via the inlet conduit;filling the cavity with cell media; andsealing the inlet tube.2. The method of claim 1 , further comprising wetting the immune-isolation device prior to filling the pouch.3. The method of claim 1 , further comprising sterilizing the loading system.4. The method of claim 3 , wherein sterilizing comprises at least one of gamma sterilization claim 3 , e-beam sterilization claim 3 , and ethylene oxide sterilization.5. The method of claim 2 , further comprising introducing saline through an opening in the disk lid.6. The method of claim 5 , further comprising introducing saline without pumps or pressure.7. The method of claim 5 , further comprising introducing an alcohol through the inlet conduit.8. The method of claim 7 , further comprising allowing the alcohol and saline to equilibrate.9. The method of claim 7 , further comprising removing a portion of the alcohol through the inlet conduit.10. The method of claim 9 , further comprising coupling a saline ...

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Номер записи: 71

12-04-2018 дата публикации

CELL ENCAPSULATION LOADING DEVICE

Номер: US20180099130A1

Автор: Bell Audrey, Evans Stephen

Принадлежит: JANSSEN BIOTECH, INC.

The present invention provides a loading fixture for loading cells, cell clusters, and media to an immune-isolation device. The loading fixture permits the loading of cells into the immune-isolation device without manual manipulation of the immune-isolation device, until the loading device is opened at the point of use, thereby minimizing the risk of contamination of the immune-isolation device. 1. A cell encapsulation system , comprising:a body comprising a base and a lid, forming a cavity therebetween, with an opening on at least one of the lid or the base; andan immune-isolation device comprising an inlet and a cell pouch, the inlet being coupled to the opening.2. The system of claim 1 , wherein the immune-isolation device is in fluid communication with the cavity.3. The system of claim 1 , wherein the body is configured to be vented.4. The system of claim 1 , wherein the lid is removable.5. The system of claim 1 , wherein the opening is on the base.6. The system of claim 1 , further comprising a blade slidably disposed in the cavity configured to cut the inlet.7. The system of claim 1 , wherein at least one of the base and the lid has a feature configured to assist with separating the base from the lid.8. The system of claim 7 , wherein the feature is ribs. The present application is a continuation of U.S. application Ser. No. 15/047,027, filed Feb. 18, 2016, and which claims the benefit of U.S. Provisional Patent Application Ser. No. 62/117,974, filed Feb. 19, 2015, all of which are incorporated herein by reference in their entirety for all purpose.The invention relates to the field of loading devices useful in loading cells into an encapsulation device. In particular, the invention relates to devices for loading cells into a medical device that will be implanted into a body.There is a clinical need for devices that encapsulate living cells and allow transplantation into a host without triggering a robust immune response from the host. In general, cellular ...

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Номер записи: 72

12-04-2018 дата публикации

Self-contained anaerobic environment-generating culture device

Номер: US20180100131A1

Автор: Adam J. Stanenas, Evan D. BRUTINEL, Jason W. Bjork, Mary B. Forester

Принадлежит: 3M Innovative Properties Co

A device for culturing anaerobic microorganisms is provided. The device comprises a body comprising a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed between the base and the coversheet. The growth compartment has a perimeter and an opening that provides liquid access to the growth compartment. A portion of the perimeter is defined by a waterproof seal. The portion includes >50% of the perimeter. A dry cold water-soluble gelling agent is adhered to the base in the growth compartment. A dry first oxygen-scavenging reagent is disposed in the growth compartment.

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Номер записи: 73

23-04-2015 дата публикации

Methods and devices for selective disruption of fatty tissue by controlled cooling

Номер: US20150112412A1

Автор: Dieter Manstein, Richard Rox Anderson

Принадлежит: General Hospital Corp

The present invention relates to methods for use in the selective disruption of lipid-rich cells by controlled cooling. The present invention further relates to a device for use in carrying out the methods for selective disruption of lipid-rich cells by controlled cooling.

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Номер записи: 74

19-04-2018 дата публикации

An apparatus for providing large amounts of gas to a fermentation broth

Номер: US20180105786A1

Автор: Aiichiro YOSHIDA, Karen HEINZE, Marshall Medoff, Robert Paradis, Thomas Craig Masterman

Принадлежит: Xyleco Inc

According to various aspects and embodiments, a system and method for aerobic fermentation of an aqueous sugar solution is provided. The system includes a vessel, at least one air diffuser in fluid communication with an interior of the vessel, and at least one blower that is configured to deliver air to the at least one air diffuser.

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Номер записи: 75

28-04-2016 дата публикации

Integrated Continuous Isolation of Fluid Streams From Sterile Process Vessels

Номер: US20160115438A1

Автор: Robert Snow

Принадлежит: Genzyme Corp

Provided herein are isolation processes and the associated hardware to allow fluid streams to be isolated from a sterilized system (e.g., a sterile process vessel) that contains a sterile process. The isolation processes described herein allow for continuous removal of fluid streams (e.g., waste streams, liquid containing recombinant therapeutic proteins) from a sterilized system (e.g., a biological manufacturing system), which provides for less manual manipulation of the sterilized system and a decreased risk of contaminating the sterilized system.

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Номер записи: 76

27-04-2017 дата публикации

Methods for harvesting mammalian cell cultures

Номер: US20170114381A1

Автор: Chetan Goudar, Henry Lin, Jonathan LULL, Nicole Le, Sean Cole, Tharmala THARMALINGAM

Принадлежит: AMGEN INC

The invention provides methods and materials for culturing mammalian cells and harvesting recombinant protein.

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Номер записи: 77

18-04-2019 дата публикации

DEVICE AND METHOD FOR MONITORING THE TEMPERATURE OF A CRYOPRESERVED BIOLOGICAL SAMPLE

Номер: US20190113396A1

Автор: FUHR Guenter R., Zimmermann Heiko

Принадлежит:

The invention relates to a method for monitoring the temperature of a cryopreserved biological sample. The invention also relates to a device for monitoring the temperature of a cryopreserved biological sample. The device () for monitoring the temperature of a cryopreserved biological sample comprises a sample container () having a receiving space () for receiving a biological sample (). The device also comprises at least one chamber () having an interior that is not fluidically connected to the receiving space () and is only partially filled with an indicator substance () with a melting temperature in a region of −20° C. to −140° C. The chamber () has a barrier () that causes the indicator substance () to move into a second sub-region () of the chamber () when the indicator substance () in a first sub-region () of the chamber is in the fluid aggregate state. 2. The device according to claim 1 , the barrier is a material claim 1 , which is arranged in the second sub-region and adjoins the first sub-region claim 1 , with a liquid-absorbing structure.3. The device according to claim 2 , whereina) the indicator substance is stored in a receptacle which tightly encloses the indicator substance in the liquid state; andb) the device has an activation part which is guided movably in relation to the receptacle and which can be moved from a starting position into an activation position, wherein the movement into the activation position brings about that the activation part, as a result of mechanical pressure, destroys the receptacle at at least one point such that the receptacle becomes permeable for the indicator substance in the liquid state.4. The device according to claim 3 , wherein the receptacle is a plastic cushion or as a glass ball.5. The device according to claim 2 , wherein a surface of the material which adjoins the first sub-region has a covering claim 2 , which is configured claim 2 , in the case of cooling of the device to a storage temperature which lies ...

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Номер записи: 78

18-04-2019 дата публикации

DEVICE AND METHOD FOR MONITORING THE TEMPERATURE OF A CRYOGENICALLY PRESERVED BIOLOGICAL SAMPLE

Номер: US20190113397A1

Автор: FUHR Guenter R., Zimmermann Heiko

Принадлежит:

The invention relates to a device for monitoring the temperature of a cryogenically preserved biological sample. The device () comprises a sample container () having a receptacle space () for accommodating a biological sample (). The device further comprises at least one chamber (), the interior () of which is not fluidically connected to the receptacle space, and which is at least partially filled with an indicator substance () having a boiling point or sublimation temperature in a range from −10° C. to −140° C. The chamber () further has at least one opening via which the indicator substance () can escape from the interior () of the chamber () upon exceeding its boiling point or sublimation point. The invention further relates to a method for monitoring the temperature of a cryogenically preserved biological sample. 1. A device for temperature monitoring of a cryopreserved biological sample , comprisinga) a sample container with a receiving space for receiving a biological sample; andb) at least one chamber, an inner space of which is not fluidically connected to the receiving space and is at least partially filled with an indicator substance, boiling temperature or a sublimation temperature of which lies in a range from −10° C. to −140° C., wherein the at least one chamber comprises at least one opening via which the indicator substance can escape from the inner space of the chamber in a case of exceeding of the boiling temperature or the sublimation temperature of the indicator substance.2. The device according to claim 1 , wherein there is a plurality of chambers at least partially filled with indicator substances claim 1 , the boiling temperature or the sublimation temperature of which lies in a range from −10° C. to −140° C. claim 1 , wherein the indicator substances in the chambers have different boiling temperatures or sublimation temperatures.3. The device according to claim 1 , a wall of the at least one chamber is transparent or semi-transparent at at ...

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Номер записи: 79

03-05-2018 дата публикации

POLYHYDROXYALKANOATE PRODUCTION METHODS AND SYSTEMS FOR SAME

Номер: US20180119181A1

Автор: Herrema Markus Donald, Kimmel Kenton

Принадлежит:

Several embodiments of the invention relate generally to a system and methods for the treatment of gaseous emissions comprising methane and one or more non-methane compounds that can influence the metabolism of methane-oxidizing microorganisms. In several embodiments, there is provided a system and methods for the treatment of methane emissions through the use of methanotrophic microorganisms to generate functionally consistent and harvestable products. Certain embodiments of the invention are particularly advantageous because they reduce environmentally-destructive methane emissions and produce harvestable end-products. 1a) providing a gas comprising methane and one or more non-methane substances that can impact the metabolism of methanotrophic microorganisms;b) providing a culture of methanotrophic microorganisms;c) providing a microorganism culture medium comprising at least a first essential nutrient and a second essential nutrient;d) exposing said culture to said gas;e) controlling the concentration of said first essential nutrient in said culture medium to a concentration sufficient to induce said methanotrophic microorganisms to produce particulate methane monooxygenase (pMMO) and/or soluble methane monooxygenase (sMMO); andf) controlling the concentration of said second essential nutrient, wherein said control of said second essential nutrient causes said methanotrophic microorganisms to produce said PHA.. A method for producing a polyhydroxyalkanoate (PHA) in a culture of methanotrophic microorganisms, the method comprising: This application is a continuation of co-pending U.S. patent application Ser. No. 14/526,228, filed on Oct. 28, 2014, which is a continuation of U.S. patent application Ser. No. 14/286, 274, filed May 23, 2014, which is a continuation of U.S. patent application Ser. No. 13/310,542, filed Dec. 2, 2011, now issued as U.S. Pat. No. 8,735,113, the entire disclosures of each of which is incorporated by reference herein.Several embodiments of ...

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Номер записи: 80

16-04-2020 дата публикации

FOAM MITIGATION IN BIOREACTORS USING ULTRASOUND

Номер: US20200115669A1

Автор: Bremer, JR. Gregory

Принадлежит: SARTORIUS STEDIM NORTH AMERICA INC.

A bioreactor for culturing of microorganisms or cells comprising: a casing defining an interior space of the bioreactor, wherein said interior space is configured to receive and hold a culture medium with microbial or cellular liquid cultures; and at least one coupling element provided on the casing for coupling with a foam mitigation device for mitigation of foam built-up in the interior space of the bioreactor during use of the bioreactor for culturing of microorganisms or cells; wherein the coupling element is configured to allow ultrasonic waves generated by the foam mitigation device act upon foam built-up in the interior space of the bioreactor. 1. A bioreactor for culturing of microorganisms or cells comprising:a casing defining an interior space of the bioreactor, wherein said interior space is configured to receive and hold a culture medium with microbial or cellular liquid cultures; andat least one coupling element provided on the casing for coupling with a foam mitigation device for mitigation of foam built-up in the interior space of the bioreactor during use of the bioreactor for culturing of microorganisms or cells;wherein the coupling element is configured to allow ultrasonic waves generated by the foam mitigation device act upon foam built-up in the interior space of the bioreactor.2. The bioreactor of claim 1 , wherein the coupling element is substantially provided in a region of the casing higher than an intended filling height of the culture medium.3. The bioreactor of claim 1 , wherein the coupling element comprises an area substantially permeable to ultrasonic waves.4. The bioreactor of claim 1 , further comprising one or more additional coupling elements for coupling with one or more foam mitigation devices.5. The bioreactor of claim 1 , wherein the bioreactor is a single-use bioreactor.6. The bioreactor of claim 1 , further comprising:at least one inlet port for introducing fluids into the interior space of the bioreactor; andat least one ...

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Номер записи: 81

25-04-2019 дата публикации

METHOD FOR IDENTIFYING TARGET BIOLOGICAL MOLECULE, BEAD FOR IDENTIFYING TARGET BIOLOGICAL MOLECULE, SET OF BEADS, AND TARGET BIOLOGICAL MOLECULE IDENTIFICATION DEVICE

Номер: US20190120789A1

Автор: Hamada Ryo, TANAKA Shuhei

Принадлежит:

A method for identifying a target biological molecule includes a step (a) of bringing a sample containing a biological molecule into contact with a plurality of beads on which a ligand capable of binding to one of a plurality of target biological molecules, and a nucleic acid for bead identification which has a specific sequence for each type of the ligand are immobilized; a step (b) of arranging the plurality of beads which have been brought into contact with the sample in each of individual reaction vessels for each bead; a step (c) of adding a reagent necessary for a nucleic acid elongation reaction to the reaction vessel in which the bead is arranged; a step (d) of performing the nucleic acid elongation reaction in the reaction vessel in which the bead is arranged; a step (e) of measuring an amount of protons generated in each of the reaction vessels during the nucleic acid elongation reaction; a step (f) of determining the occurrence or non-occurrence of nucleic acid elongation in each of the reaction vessels based on the amount of protons generated; and a step (g) of performing a nucleic acid elongation reaction in the reaction vessel in which the bead is arranged using the nucleic acid for bead identification as a template, identifying the sequence of the nucleic acid for bead identification for each reaction vessel based on the amount of protons generated in each reaction vessel during the nucleic acid elongation reaction using the nucleic acid for bead identification as a template, and identifying the type of the ligand for each reaction vessel based on the sequence of the nucleic acid for bead identification identified for each reaction vessel. 1. A method for identifying a target biological molecule , comprising:(a) bringing a sample containing biological molecules into contact with a plurality of beads on which a ligand capable of binding to one of a plurality of target biological molecules, and a nucleic acid for bead identification which has a specific ...

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Номер записи: 82

25-04-2019 дата публикации

TARGET ANALYSIS TOOL AND TARGET ANALYSIS METHOD

Номер: US20190120832A1

Автор: AKITOMI Jou, Horii Katsunori, Kaneko Naoto, Waga Iwao, Yoshida Yoshihito

Принадлежит: NEC Solution Innovators, Ltd.

A target analysis tool and a target analysis method that allow easily analysis of a target. The first target analysis tool includes: a first chamber; a second chamber; and a third chamber. The first chamber, the second chamber, and the third chamber are disposed continuously in this order. The first chamber contains, as a first reagent, an immobilized first binding substance obtained by immobilizing, on a carrier, a first binding substance that binds to a target. The second chamber contains, as a second reagent, a labeled second binding substance obtained by binding a labeling substance to a second binding substance that binds to the first binding substance. The third chamber is a detection section in which the labeled second binding substance is detected. 18.. (canceled)9. A target analysis method using a target analysis tool comprising a first chamber; a second chamber; and a third chamber , wherein the first chamber , the second chamber , and the third chamber are disposed continuously in this order , the first chamber contains , as a first reagent , an immobilized second binding substance obtained by immobilizing , on a carrier , a second binding substance that binds to a first binding substance that binds to a target , the second chamber contains , as a second reagent , a labeled first binding substance obtained by binding a labeling substance to the first binding substance , the third chamber is a detection section in which the labeled first binding substance is detected , a first partition wall is provided between the first chamber and the second chamber , a second partition wall is provided between the second chamber and the third chamber , the first chamber is configured so that a specimen holder having a specimen can be inserted from an outside of the first chamber to an inside of the first chamber , the first partition wall is a partition wall that is broken upon contact with a tip of the specimen holder inserted into the first chamber , and the second ...

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Номер записи: 83

27-05-2021 дата публикации

Cell culture device

Номер: US20210155887A1

Автор: Shunmei Chiba, Takaki USHIFUSA, Young-Jin Lee

Принадлежит: Fullstem Co Ltd

Provided is a cell culture device that has a simple structure and ensures sufficient nutrient supply to cells and oxygenation of the cells to thereby enable mass cell culture. The cell culture device comprises: a cell culture container which is an approximately cylindrical body provided with a flat bottom part at the lower end; a dish-shaped body having an approximately disc shape which is provided with a plurality of magnetic attraction members, said magnetic attraction members being positioned in the circumferential part at equal intervals, and horizontally disposed in a non-contact state within a hollow space inside the cell culture container; and a cyclic body which is provided with a plurality of magnetic attraction members and positioned outside the cell culture container so that the cell culture container is located within the cycle thereof. The cyclic body and the dish-shaped body move vertically in magnetic conjunction to thereby agitate a medium and supply nutrients to cells.

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Номер записи: 84

12-05-2016 дата публикации

Lactobacillus brevis g-101 strain and use thereof

Номер: US20160129057A1

Автор: Byeong-Gon Lee, Go-Eun SHIN, Hong Ryeol Jeon, Se-Young Kim, Yoon-Mo KANG

Принадлежит: CTC BIO Inc

The present invention relates to a novel Lactobacillus brevis G-101 strain capable of decomposing monosodium L-glutamate (MSG), and a functional health food, a pharmaceutical composition, or a food product comprising the same as an active ingredient. More specifically, the strain is effective in reducing in vivo blood MSG levels of animals and attenuating MSG Symptom Complex, and thus can be used in a functional health food, a pharmaceutical composition, or a food product aiming to prevent in vivo absorption of MSG, which is known to be harmful, and improve the MSG Symptom Complex.

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Номер записи: 85

10-05-2018 дата публикации

AUTOMATIC DEVICE FOR CULTURING CELL AND OPERATING METHOD THEREOF

Номер: US20180127695A1

Автор: LEE Jinku, Nam Dohyun, RYU Gyuha

Принадлежит: SAMSUNG LIFE PUBLIC WELFARE FOUNDATION

Provided herein is an automatic cell culture device including an incubator configured to contain at least one container for culturing cells, a microscope configured to observe a state of the cell in the container, a robot arm configured to move the container, a liquid handler configured to introduce liquid into or discharge liquid from the container, and a control device configured to control an operation of at least one of the incubator, the microscope, the robot arm, and the liquid handler. 1. An automatic cell culture device comprising:an incubator configured to contain at least one container for culturing cells;a microscope configured to observe a state of the cell in the container;a robot arm configured to move the container;a liquid handler configured to suction liquid from the container; anda control device configured to control an operation of at least one of the incubator, the microscope, the robot arm, and the liquid handler.2. The automatic cell culture device of claim 1 , further comprising:a repository configured to store the container to be moved by the robot arm; anda centrifugal separator configured to separate a particle of a material included in the container using centrifugal force.3. An operating method of an automatic cell culture device claim 1 , the operating method comprising:extracting at least one container that contains a cell cultured in an incubator for a predetermined time from the incubator using a robot arm;observing a state of the cell in the extracted container using a microscope;selecting an operation protocol for the container based on a result of the observation; anddriving the robot arm according to the selected operation protocol.4. The operating method of claim 3 , wherein the operation protocol for the container is at least one of a protocol for an operation of reinserting the container into the incubator claim 3 , a protocol for an operation of injecting a culture medium into the container claim 3 , and a protocol for an ...

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Номер записи: 86

10-05-2018 дата публикации

Culturing Device

Номер: US20180127696A1

Автор: TAGUCHI Atsushi, TAKEUCHI Masakatsu

Принадлежит:

A compact culture device capable of performing large-scale culture in an aseptic state. A culture device has a culture bag which has ports through which a liquid flows and enables cell culture, a bag holding portion having holding plates holding the culture bag and rotating shafts, a rotation mechanism supporting and rotating the rotating shaft, a liquid supply/discharge mechanism supplying and discharging a liquid to/from the culture bag, and a rotation control portion controlling the rotation of the rotation mechanism. The rotation control portion brings the bag holding portion into a first position in parallel with the approximately horizontal direction according to a first information, brings the bag holding portion into a second position when a culture medium in the culture bag is caused to flow out of the port according to a second information, and brings the bag holding portion into a third position in parallel with the approximately vertical direction at least when a cell suspension in the culture bag is caused to flow out of the port according to a third information. 1. A culture device comprising:a culture vessel which has port through which a liquid flows and enables cell culture in an internal space;a vessel holding portion having holding plate holding the culture vessel and rotating shaft;a rotation mechanism supporting and rotating the rotating shaft;a liquid supply/discharge mechanism supplying and discharging a liquid to/from the culture vessel; anda rotation control portion controlling rotation of the rotation mechanism, whereinthe rotation control portion brings the vessel holding portion into a first position in which supporting surface supporting the culture vessel in the holding plate are in parallel with an approximately horizontal direction according to a first information indicating that cell culture is performed in the culture vessel, andbrings the vessel holding portion into a second position in which the supporting surface are not in ...

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Номер записи: 87

11-05-2017 дата публикации

Colorectal cancer detection kit or device, and detection method

Номер: US20170130274A1

Автор: Atsushi Ochiai, Hiroko SUDO, Hitoshi Nobumasa, Junpei KAWAUCHI, Motohiro Kojima, Satoko Kozono, Satoshi Kondou

Принадлежит: National Cancer Center Japan, TORAY INDUSTRIES INC

It is intended to provide a kit or a device for the detection of colorectal cancer and a method for detecting colorectal cancer. The present invention provides a kit or a device for the detection of colorectal cancer, comprising a nucleic acid capable of specifically binding to a miRNA in a sample from a subject, and a method for detecting colorectal cancer, comprising measuring the miRNA in vitro.

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Номер записи: 88

02-05-2019 дата публикации

METHOD OF REPLACING LIQUID MEDIUM AND FLOW CHANNEL DEVICE FOR THE METHOD

Номер: US20190126278A1

Автор: Arai Noriaki

Принадлежит: TOPPAN PRINTING CO., LTD.

A method of replacing a liquid medium including feeding in a flow direction a plurality of liquids including a first liquid medium having a target particle dispersed therein and a second liquid medium such that a laminar flow is formed, and that the laminar flow has laminar flow segments including a first laminar flow segment formed by the first liquid medium and a second laminar flow segment formed by the second liquid medium, applying an external force to the laminar flow such that the target particle is moved from the first laminar flow segment to the second laminar flow segment, and recovering a laminar flow fraction including the target particle from the second laminar flow segment from a recovery surface which is perpendicular to the flow direction and separated from a cross section of the first laminar flow segment. 1. A method of replacing a liquid medium , comprising:feeding in a flow direction a plurality of liquids including a first liquid medium having a target particle dispersed therein and a second liquid medium such that a laminar flow is formed, and that the laminar flow has a plurality of laminar flow segments including a first laminar flow segment formed by the first liquid medium and a second laminar flow segment formed by the second liquid medium;applying an external force to the laminar flow such that the target particle is moved from the first laminar flow segment to the second laminar flow segment; andrecovering a laminar flow fraction including the target particle from the second laminar flow segment from a recovery surface which is perpendicular to the flow direction and separated from a cross section of the first laminar flow segment.2. The method of claim 1 , wherein the first laminar flow segment is adjacent to and in contact with the second laminar flow segment.3. The method of claim 1 , wherein the laminar flow segments include a third laminar flow segment formed by a third liquid medium claim 1 , and the third laminar flow segment is ...

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Номер записи: 89

01-09-2022 дата публикации

CONTAINER FOR SEPARATING LIVE CELLS

Номер: US20220275316A1

Автор: MATSUDA Isamu, OHASHI Fumiya, Oyama Kenji, YUKI Risa

Принадлежит: TERUMO KABUSHIKI KAISHA

A container is disclosed for isolating living cells. The container for crushing a sample by pressing includes a container main body having an opening part and a partition which separates an upper space and a lower space of the container main body, in which at least a part of the partition has a portion which is not horizontal with respect to the bottom of the container. 1. A container for crushing a sample by pressing , the container comprising:a container main body having an opening part;a partition which separates an upper space and a lower space of the container main body; andwherein at least a part of the partition has a portion which is not horizontal with respect to the bottom of the container.2. The container according to claim 1 , wherein the lower space is configured so that a protruding part which protrudes towards the opening part is formed by the partition.3. The container according to claim 1 , wherein the partition has a communicating hole at the apex of the partition.4. The container according to claim 1 , wherein the partition has a shape that resembles an inverted V or a shape of two tilted lines converging at a top.5. The container according to claim 4 , wherein slopes of the shape that resembles the inverted V and/or the shape of two tilted lines converging at the top are asymmetrical.6. The container according to claim 1 , further comprising:a sealed part.7. The container according to claim 1 , wherein the partition is formed by seal formation.8. The container according to claim 1 , further comprising:a tissue derived from a living body.9. The container according to claim 1 , wherein the container is configured to isolate CD56-positive cells.10. A system for isolating living cells claim 1 , the system comprising:a solution having tissue derived from a living body; anda container configured to receive the solution, the container including a main body having an opening part, a partition which separates an upper space and a lower space of the ...

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Номер записи: 90

02-05-2019 дата публикации

Device for biological material detection, detection apparatus for biological material detection, method for measuring ion current, and method for identifying biological material

Номер: US20190128888A1

Автор: Kazumichi Yokota, Makusu Tsutsui, Masateru Taniguchi, Masayoshi Tanaka, Mina Okochi, Noritada Kaji, Takao Yasui, Tomoji Kawai, Yoshinobu Baba, Yuji Miyahara, Yukichi HORIGUCHI

Принадлежит: Nagoya University NUC, Osaka University NUC, Tokyo Institute of Technology NUC, Tokyo Medical and Dental University NUC

A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.

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Номер записи: 91

03-06-2021 дата публикации

Sample container for stabilizing and aligning excised biological tissue samples for ex vivo analysis

Номер: US20210163864A1

Автор: Chao FAN, David Rempel, Elizabeth A. MUNRO, James Jackson

Принадлежит: Perimeter Medical Imaging Inc

Embodiments described herein relate to an apparatus for positioning and securing an excised biological tissue specimen for imaging and analysis. In some embodiments, an apparatus includes a sample bag defining an inner volume configured to receive a biological tissue sample, and a sealing member coupled to the sample bag. An imaging window is disposed and configured to be placed in contact with at least a portion of the biological tissue sample, and a positioning member is coupled to the imaging window and is configured to be disposed against the sealing member to substantially seal the inner volume. The positioning member includes a vacuum port disposed and configured to be aligned with a vacuum source to withdraw air from the inner volume of the sample bag.

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Номер записи: 92

23-04-2020 дата публикации

Information processing apparatus, information processing method, program, and cell observation system

Номер: US20200125030A1

Автор: Hirokazu Tatsuta, Takeshi Kunihiro

Принадлежит: Sony Corp

An information processing apparatus according to an embodiment of the present technology includes: a calculation unit; and an amplitude replacement unit. The calculation unit repeatedly executes first light propagation calculation for propagating, from a sensor surface of an image sensor to a support surface that supports a cell to be observed, a first complex amplitude distribution that includes a light intensity distribution of a hologram of the cell obtained on the sensor surface, and second light propagation calculation for propagating, from the support surface to the sensor surface, a second complex amplitude distribution obtained as a result of the first light propagation calculation. The amplitude replacement unit replaces, in the second light propagation calculation, an amplitude component of the second complex amplitude distribution with a predetermined representative amplitude value at least once.

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Номер записи: 93

09-05-2019 дата публикации

Biomolecule Measuring Device

Номер: US20190137431A1

Автор: Michiru Fujioka, Yoshimitsu Yanagawa, Yusuke Goto

Принадлежит: Hitachi High Technologies Corp

An object of the present invention is to provide a biomolecule measuring device that can decrease the influence of crosstalk between chambers. A biomolecule measuring device according to the present invention supplies, to electrodes equipped on chambers, voltages modulated differently to each other.

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Номер записи: 94

24-05-2018 дата публикации

CELL TREATMENT METHOD, LASER PROCESSING MACHINE, AND CELL CULTURE VESSEL

Номер: US20180142193A1

Автор: KANAMORI Toshiyuki, Matsumoto Junichi, Nishi Norio, SUMARU Kimio, Suzuki Masami

Принадлежит:

A method of killing specific cells from among a group of cells cultured in a culture vessel by quick and brief laser treatment, the cell culture vessel comprising a main body and a to-be-irradiated layer attached to the main body, the to-be-irradiated layer containing an ingredient capable of absorbing laser light upon laser irradiation, the group of cells being cultured on the surface of the to-be-irradiated layer, the method comprising: 1. A cell treatment method of killing specific cells from among a group of cells cultured in a cell culture vessel , the cell culture vessel comprising a main body and a to-be-irradiated layer attached to the main body , the to-be-irradiated layer containing an ingredient capable of absorbing laser light upon laser irradiation , the group of cells being cultured on the surface of the to-be-irradiated layer , the method comprising:applying laser light to a partial area of the to-be-irradiated layer directly below the specific cells.2. The cell treatment method according to claim 1 , wherein the laser light applied to the partial area of the to-be-irradiated layer directly below the specific cells has the right level of output or energy amount to kill the specific cells not instantly but after a certain period of time.3. The cell treatment method according to claim 1 , wherein the laser light applied to the partial area of the to-be-irradiated layer directly below the specific cells has the right level of output or energy amount not to kill the specific cells instantly and is applied multiple times.4. The cell treatment method according to for dividing the group of cells cultured in the cell culture vessel into a plurality of portions claim 1 , wherein the laser light is applied to a partial area of the to-be-irradiated layer directly below the boundary between any two portions of the group of cells.5. A laser processing machine for killing specific cells from among a group of cells cultured in a cell culture vessel claim 1 , the ...

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Номер записи: 95

07-05-2020 дата публикации

Fish egg processing apparatus

Номер: US20200137988A1

Автор: Akira Nakanishi, Saori Nakai, Yuji Yamanaka

Принадлежит: Sinfonia Technology Co Ltd

There is provided a fish egg processing apparatus capable of performing a predetermined processing on fertilized eggs with higher efficiency. A gene injection device 1 which is a fish egg processing apparatus according to the present invention includes a processing water tank 8 which processes collected fish eggs e and a processing device for injecting a predetermined substance into the fish eggs e positioned in the processing water tank 8, wherein the processing water tank 8 includes: a region prior to processing 81 where the width dimension is set such that a plurality of the fish eggs e are not allowed to lie side by side and form a line in order to house the fish eggs e prior to processing; and a region after processing 82 for housing the fish eggs e on which a predetermined processing has been performed.

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Номер записи: 96

17-06-2021 дата публикации

Matrix Fermentation Systems and Methods for Producing Microbe-Based Products

Номер: US20210180000A1

Автор: Alibek Moldakozhayev, Ken Alibek, Maja MILOVANOVIC, Sean Farmer, Sharmistha MAZUMDER

Принадлежит: Locus IP Co LLC

The subject invention provides methods of producing advantageous microbes and/or by-products using a modified form of solid-state fermentation, or matrix fermentation. In particular, the methods utilize foodstuff mixed with liquid nutrient medium to produce a three-dimensional scaffold substrate having ample surface area on which the microbes can grow. The methods can be used to cultivate yeasts, fungi and bacteria at high concentrations without susceptibility to total contamination. The subject invention can be used in remote locations and can be transported with ease.

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Номер записи: 97

07-06-2018 дата публикации

METHOD FOR ASSISTING THERMALLY-INDUCED CHANGES

Номер: US20180154279A1

Автор: FAN Chao, Ninham Barry, Pashley Richard, Shahid Muhammad, Xue Xinkai

Принадлежит:

Disclosed herein is a method for assisting a thermally-induced change to a nano-sized solute or dispersed-phase in a liquid. The method comprises the step of passing gas bubbles through the liquid, the gas in the gas bubbles having a temperature higher than the bulk temperature of the liquid. 1. A method for assisting a thermally-induced change to a nano-sized solute or dispersed-phase in a liquid , the method comprising the step of:passing gas bubbles through the liquid, the gas in the gas bubbles having a temperature higher than the bulk temperature of the liquid.2. The method according to claim 1 , wherein the liquid is water or an aqueous solution.3. The method according to claim 1 , wherein the bulk temperature of the liquid is less than the temperature which would cause the thermally-induced change to the nano-sized solute or dispersed-phase.4. The method according to claim 1 , wherein the nano-sized solute or dispersed-phase has a diameter of less than 500 nm.5. The method according to claim 4 , wherein the nano-sized solute or dispersed-phase has a diameter of 0.5 nm to 200 nm.6. The method according to claim 1 , wherein the bubbles have a diameter of 0.1 mm to 7 mm.7. The method according to claim 6 , wherein the bubbles have a diameter of 1 mm to 3 mm.8. The method according to claim 7 , wherein the bubbles have a diameter of approximately 2 mm.9. The method according to claim 1 , wherein the nano-sized solute or dispersed-phase is selected from the group consisting of thermally decomposable inorganic solutes claim 1 , thermally decomposable organic solutes claim 1 , enzymes claim 1 , antibiotics and hormones.10. The method according to claim 9 , wherein the nano-sized solute or dispersed-phase is a thermally decomposable inorganic solute.11. The method according to claim 10 , wherein the thermally decomposable inorganic solute is an ion of an inorganic salt.12. The method according to claim 11 , wherein the ion is selected from the group consisting of ...

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Номер записи: 98

07-06-2018 дата публикации

DARK-ENVIRONMENT SIMULTANEOUS CULTURING-OBSERVING APPARATUS

Номер: US20180156722A1

Автор: MIYASHITA Mitsuyoshi

Принадлежит:

A dark-environment simultaneous culturing-observing apparatus includes: an observation-subject holding unit for holding an observation subject; a dark-environment culturing chamber for culturing, in a dark environment, bacteria in the observation subject held by the observation-subject holding unit; an infrared light source for irradiating, with infrared light, the observation subject held by the observation-subject holding unit kept in the dark-environment culturing chamber; and an infrared light camera for photographing the observation subject irradiated with infrared light from the infrared light source. 1. A dark-environment simultaneous culturing-observing apparatus comprising:an observation-subject holding unit for holding an observation subject;a dark-environment culturing chamber for culturing, under a dark environment, bacteria in the observation subject held by the observation-subject holding unit;an infrared light source for irradiating, with infrared light, the observation subject held by the observation-subject holding unit kept in the dark-environment culturing chamber; andan infrared light camera for photographing the observation subject irradiated with infrared light from the infrared light source.2. The dark-environment simultaneous culturing-observing apparatus according to claim 1 , further comprising a control unit for performing multiple photographing control for continuously photographing the observation subject with the infrared light camera over multiple times.3. The dark-environment simultaneous culturing-observing apparatus according to claim 1 , wherein claim 1 , as the infrared light source claim 1 , near-infrared light is irradiated.4. The dark-environment simultaneous culturing-observing apparatus according to claim 1 , wherein the infrared light source is provided with a filter for blocking a predetermined wavelength band of infrared light.5. The dark-environment simultaneous culturing-observing apparatus according to claim 1 , wherein ...

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Номер записи: 99

08-06-2017 дата публикации

Bioreactor for production and harvesting of microalgae

Номер: US20170158995A1

Автор: Jan Erik Kyrkjebø, Jan Fredrik GARVIK

Принадлежит: Biosystems As

A bioreactor ( 30 ) for production and harvesting of microalgae is described, comprising a reactor basin ( 32 ) in the form of a tank arranged to receive CO2 and water as well as algae, and which is equipped with at least one outlet for harvesting of algal biomass, where the reactor basin ( 32 ) comprises a rotating beam ( 54 ) equipped with one or more tillable mixing grates ( 60 ) and harvesting grates ( 62 ).

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Номер записи: 100