Preparation of fructose-1,6-disphosphate

The process provides the enzymatic phosphorylation of glucose to FDP by means of yeast immobilized by glutaraldehyde and the separation of FDP by ultrafiltration. A suspension of glutaraldehyde-treated yeast is fed to a fermenter, with addition of a nutrient mixture consisting of dextrose (1M), sodium phosphate (0.5 M), magnesium chloride (10 mM) and phosphoric acid until pH 6.5. The mixture is re-circulated through a hollow fiber ultrafiltration unit to separate the FDP that forms during the phosphorylation reaction.

PREPARATION OF FRUCTOSE-1,6-DIPHOSPHATE

FRUCTOSEL 6-DIPHOSPHATE

PROCEDURE FOR THE PRODUCTION OF CELLULASE AND PLANT FOR THE EXECUTION OF THE PROCEDURE

PROCEDURE FOR THE PRODUCTION OF CELLULASE AND PLANT FOR THE EXECUTION OF THE PROCEDURE

Bioreactor for obtaining secondary plant ingredients.

Es wird ein Bioreaktor 1 zur Gewinnung sekundärer Pflanzen-Inhaltsstoffe vorgestellt. Dieser besteht aus einer beutelförmigen, flexiblen Hülle 2, welche oben durch einen formstabilen Deckel 10 und unten durch einen formstabilen Boden 20 abgeschlossen ist. Im Innern, innerhalb der Hülle 2, ist mindestens eine feste Trägerplatte 30 mittels einer am Deckel (10) befestigten Aufhängung 31 frei hängend angeordnet. Der Bioreaktor 1 kann über Sprühdüsen 11 im Deckel 10 mit Nährstoffen und Gasen beaufschlagt werden. Ein Auslass 21 im Boden 20 ist für den Abfluss der verbleibenden Nährstoffflüssigkeit und dem gewünschten metabolischen Produkt vorgesehen. Der Bioreaktor 1 mit dem ganzen Inhalt ist zusammenlegbar.

Continuous culture of aerobic microorganisms at high cell density

Process for the continuous culture of aerobic organisms at high cell density. This is carried out in a vortex reactor with power input of 4-40 kW/m<3> and a vortex size:inlet dia. ratio of <10 and comprises:(a) injecting a liq. medium tangentially into the upper part of the reactor with a centrifugal force of <= 25 x g;(b) mixing an O2-contg. gas with the medium or introducing it via a jacket around the reactor;(c) withdrawing the liq. medium from the bottom of the reactor and readjusting it to the process conditions in an external recycling system, and(d) withdrawing accumulated gas from the top of the reactor. Also claimed is the appts. for carrying out the process above, where the reactor is integrated into a recycling system with elements for controlling the process conditions. The reactor has a gas outlet at the top, a liq. outlet at the bottom and a tangential inlet connected to a recycling pump whose power input provides the required centrifugal force. Aeration is provided by a device ...

PERFUSION BIOREACTOR AND ASSOCIATED METHODS OF USING

METHOD OF PREPARATION Of an AQUEOUS SOLUTION, PRACTICALLY DEPRIVED OF CELLS, ASPARTASE AND ITS APPLICATION TO the MANUFACTURE OF the ASPARTIC ACID

ON PRODUIT DE L'ACIDE ASPARTIQUE, PAR VOIE ENZYMATIQUE, A PARTIR DE FUMARATE D'AMMONIUM EN PRESENCE D'UNE ENZYME, L'ASPARTASE. ON PREPARE UNE SOLUTION D'ASPARTASE DEPOURVUE DE CELLULES EN FORMANT UNE SUSPENSION AQUEUSE DE CELLULES MICROBIENNES CONTENANT DE L'ASPARTASE, EN FAISANT INCUBER CETTE SUSPENSION DE CELLULES, EN CENTRIFUGEANT LA SUSPENSION DE CELLULES POUR FORMER UNE SOLUTION CLARIFIEE QUI RENFERME LA MAJEURE PARTIE DE L'ACTIVITE ASPARTASE PRESENTE A L'ORIGINE DANS LES CELLULES, ET EN AJOUTANT A LA SUSPENSION DE CELLULES OU A LA SOLUTION CLARIFIEE UNE QUANTITE SUFFISANTE D'UN SEL STABILISATEUR DE L'ASPARTASE POUR RENDRE HYPERTONIQUE LA SOLUTION CLARIFIEE. L'ASPARTASE RECUEILLIE DE CETTE MANIERE EST PARTICULIEREMENT UTILE DANS LES REACTEURS A ENZYMES IMMOBILISES POUR LA FABRICATION DE L'ACIDE ASPARTIQUE. ASPARTIC ACID IS PRODUCED ENZYMATICALLY FROM AMMONIUM FUMARATE IN THE PRESENCE OF AN ENZYME, ASPARTASE. A CELL-FREE ASPARTASE SOLUTION IS PREPARED BY FORMING AN AQUEOUS MICROBIAL CELL SUSPENSION CONTAINING ASPARTASE, INCUBATING THIS CELL SUSPENSION, CENTRIFUGING THE CELL SUSPENSION QUARTERY CLARIFIED AS A CLARIFIED CELL SUSPENSION TO FORM A CLARIFIED SOLUTION. ASPARTASE ACTIVITY ORIGINALLY PRESENT IN THE CELLS, AND BY ADDING TO THE CELL SUSPENSION OR THE CLARIFIED SOLUTION A SUFFICIENT AMOUNT OF A STABILIZING SALT OF ASPARTASE TO MAKE THE CLARIFIED SOLUTION HYPERTONIC. THE ASPARTASE COLLECTED IN THIS MANNER IS PARTICULARLY USEFUL IN IMMOBILIZED ENZYME REACTORS FOR THE MANUFACTURE OF ASPARTIC ACID.

PHOTOBIOREACTOR

A method for non-invasive growth measurement of organisms in an apparatus for growing and harvesting organisms or substances derived from such organisms. The organisms are comprised in an aqueous medium in a vessel, the method comprising the steps of: a) Providing means for measuring the opacity of the aqueous medium in order to estimate a growth rate of the organisms; b) The means for measuring the opacity are arranged in the apparatus such that they are located at predetermined locations relative to the vessel; c) The means for measuring the opacity are calibrated to account for refraction, absorbance and reflection caused by the vessel at the predetermined location. Finally the method also allows for measuring the opacity of the aqueous medium at the predetermined location.

SYSTEM, APPARATUS AND METHOD FOR BIOMOLECULES PRODUCTION

An automated method for the production of cells and/or biomolecules such as protein or peptides includes culturing cells in at least one high cell density bioreactor, thereby fluidly connecting said bioreactor with a culture medium supply and a gas or gaseous mixture; fluidly connecting said bioreactor with a downstream unit; and growing cells to a density at least 50 million cells per ml. The total volume of the bioreactor is at least 10 liters. A system suitable for implementation of the automated method above is a small-scale and cupboard-sized system, which can be placed in a portable clean room.

Continuous culture of aerobic microorganisms at high cell density

Process for the continuous culture of aerobic organisms at high cell density. This is carried out in a vortex reactor with power input of 4-40 kW/m<3> and a vortex size:inlet dia. ratio of <10 and comprises:(a) injecting a liq. medium tangentially into the upper part of the reactor with a centrifugal force of <= 25 x g;(b) mixing an O2-contg. gas with the medium or introducing it via a jacket around the reactor;(c) withdrawing the liq. medium from the bottom of the reactor and readjusting it to the process conditions in an external recycling system, and(d) withdrawing accumulated gas from the top of the reactor. Also claimed is the appts. for carrying out the process above, where the reactor is integrated into a recycling system with elements for controlling the process conditions. The reactor has a gas outlet at the top, a liq. outlet at the bottom and a tangential inlet connected to a recycling pump whose power input provides the required centrifugal force. Aeration is provided by a device ...

Process and apparatus for enzyme production using bacterium harboring recombinant plasmids

The process comprises culturing a host bacterium into which enzyme genes and a control gene having an apo-repressor have been introduced, so that formation of enzyme is repressed while the bacterium grows in a culture medium containing a co-repressor, removing the co-repressor from the culture solution and subsequently allowing the host bacterium to produce the enzyme in the culture medium containing no co-repressor. An apparatus for producing an enzyme includes a fermentor 1 a cylindrical filter 11 for removing the co-repressor from circulating culture solution from the fermentor, and a culture medium reservoir 26 for feeding fresh culture medium containing no co-repressor into the fermentor 1.

CULTURE APPARATUS FOR ENZYME PRODUCTION

APPARATUS FOR ENZYME PRODUCTION

Fermentation process

VERFAHREN ZUR GEWINNUNG VON CELLULASE UND ANLAGE ZUR DURCHFUEHRUNG DES VERFAHRENS

Method for producing cellulase, and apparatus for said method

A method for producing a cellulase, comprising steps (1) to (3) mentioned below, wherein the resultant cellulase can be used effectively in washing agents and for the hydrolysis of starches, the hydrolysis of celluloses and so on: (1) filtrating an aqueous solution of a cellulase originated from a filamentous fungus through an ultrafiltration membrane having a cut-off molecular weight of 100,000 to 200,000 to produce a filtrate and a concentrated enzyme solution that is a non-filtrate; (2) filtering the filtrate produced in step (1) through a second ultrafiltration membrane having a cut-off molecular weight of 5,000 to 50,000 to produce a second concentrated enzyme solution that is a non-filtrate; and (3) mixing the concentrated enzyme solutions produced in steps (1) and (2) together to produce a cellulase originated from the filamentous fungus.

Apparatus for preparing enzyme-immobilized beads and method for preparing enzyme-immobilized beads using same

The present invention relates to an apparatus for preparing enzyme-immobilized beads used for preparation of tagatose, and a method for preparing enzyme-immobilized beads using the same. More specifically, the present invention relates to an apparatus for preparing enzyme-immobilized beads, comprising a nozzle with an inside diameter of 0.1-1 mm having a cylindrical lower end and comprising a cut-type liquid outlet (cut perpendicularly to the vertical axis of the nozzle) formed at the lower end, and a method for preparing enzyme-immobilized beads using the same.

FERMENTATION PROCESSES USING SCRAPED TUBULAR FERMENTOR

APPARATUS FOR CONVERTING INTO ADENOSINE-5'- TRIPHOSPHATE

Enzyme preparation dosing tank

PROCEDE ET INSTALLATION DE PRODUCTION EN CONTINU DE FRUCTOSE-1,6-DIPHOSPHATE AU MOYEN DE LEVURE IMMOBILISEE

L'INVENTION CONCERNE UN PROCEDE DE PHOSPHORYLATION ENZYMATIQUE DE GLUCOSE EN FDP AU MOYEN DE LEVURE IMMOBILISEE AU GLUTARALDEHYDE ET LA SEPARATION DU FDP PAR ULTRAFILTRATION. ON INTRODUIT UNE SUSPENSION DE LEVURE TRAITEE AU GLUTARALDEHYDE DANS UN FERMENTEUR1, EN AJOUTANT UN MELANGE NUTRITIF CONSTITUE PAR DU DEXTROSE (1M), DU PHOSPHATE DE SODIUM (0,5), DU CHLORURE DE MAGNESIUM (10MM) ET DE L'ACIDE PHOSPHORIQUE JUSQU'A PH 6,5. ON RECYCLE LE MELANGE A TRAVERS UNE UNITE D'ULTRAFILTRATION A FIBRES CREUSES3 POUR SEPARER LE FDP QUI SE FORME PENDANT LA REACTION DE PHOSPHORYLATION.

bioreactor perfusion and related methods of use

PRODUCTION OF ASPARTASE

Fermentation processes using scraped tubular fermentor

Fermentation of a substrate by microorganisms capable of utilizing the substrate for growth is improved by conveying a fermentation medium including the substrate and microoganisms through a hollow tube with the assistance of an internal wall-scraper.

Process for production of lignolytic enzymes by phanerochaete chrysosporium

СПОСОБ И СИСТЕМЫ ДЛЯ ОПТИМИЗАЦИИ СИСТЕМЫ ПЕРФУЗИОННОЙ КУЛЬТУРЫ КЛЕТОК

1. Система перфузионного биореактора для культуры, которая включает:биореактор, сконструированный для содержания тканевой культуральной жидкости и клеток, которые культивируются;устройство для удержания клеток, сконструированное для получения тканевой культуральной жидкости, содержащей клетки из биореактора, отделения некоторого количества клеток от тканевой культуральной жидкости, обеспечения сбора тканевой культуральной жидкости и клеток и осуществления рециркуляции полученной тканевой культуральной жидкости и клеток к биореактору;где система имеет исходную скорость перфузии, исходный объем биореактора, исходный объем устройства для удержания клеток и исходное объемное соотношение исходного объема биореактора и исходного объема устройства для удержания клеток; где либоисходная скорость перфузии снижается, что приводит к повышению времени пребывания клеток в биореакторе и устройстве для удержания клеток, либоисходный объем биореактора повышается или исходный объем устройства для удержания снижается, или оба, что приводит к повышению исходного соотношения объема.2. Система перфузионного биореактора для культуры в соответствии с п. 1, где исходная скорость перфузии снижается, что приводит к повышению времени пребывания клеток в биореакторе и устройстве для удержания клеток, а исходный объем биореактора повышается или исходный объем устройства для удержания клеток снижается, или оба, что приводит к повышению исходного объемного соотношения.3. Система перфузионного биореактора для культуры в соответствии с п. 2, где повышение исходного объемного соотношения происходит приблизительно пропорционально РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (51) МПК C12M 1/00 (13) 2015 117 547 A (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2015117547, 09.10.2013 (71) Заявитель(и): БАЙЕР ХЕЛСКЕА ЛЛК (US) Приоритет(ы): (30) Конвенционный приоритет: 10.10.2012 US 61/712,190 (85) Дата начала рассмотрения заявки PCT на национальной фазе: ...

PROCEDURE FOR THE PRODUCTION OF LIGNOLYTI ENZYMES OF MEANS PHANEROCHAETE CHRYSOSPORIUM

PROCESS FOR THE PRODUCTION OF LIGNOLYTIC ENZYMES BY MEANS OF PHANEROCHAETE CHRYSOSPORIUM

Perfusion bioreactor and related methods of use

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

Animal enzyme preparation pepsin production plant

Animal enzyme preparation pepsin production plant Abstract 5 Animal enzyme preparation pepsin production plant, including: tank (F101), agitator (DI01), transfer pump (JI01), condenser (ClOl), tank 2 (F102), precipitator (LIO), pulverizator (L102); the connection between the various components of the production plant is: tankI (F101) is connected to agitator (D101), condenser (C101) is connected to agitator (D101), agitator (DI01) is connected to transfer pump (J101), 10 transfer pump (JIO) is connected to precipitator (L1O), tank2 (F102) is connected to sedimentation tank (L1O), precipitator (LIO) is connected to pulverizator (L102); among them, cone bottom thickness of tank (FlOl) is 7-9 mm. Figure 1 f/I - ofl~ C 0- C o - -~ -. o - a ~, o~ ~ ~ -, a a 0 x *0 ~- t~ - = = = = Figure ...

PERFUSION BIOREACTOR AND RELATED METHODS OF USE

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

APPARATUS AND METHOD FOR DEHYDRATING BIOLOGICAL MATERIALS

An apparatus and method for microwave vacuum-drying of temperature- sensitive biological materials on a continuous flow-through basis, in which the materials are frozen, ground to frozen particles, dehydrated to a powder, and the powder collected. The apparatus (10) has a microwave generator (12) and waveguide (14), a freezing chamber (46) with a grinder (52), a rotatable dehydration chamber (18) in or adjacent to the waveguide, and a powder collector (82) to receive the powdered biological material. The apparatus operates under reduced pressure provided by a vacuum system (92) coupled to the powder collector (82).

Production of aspartase

Aspartic acid is enzymatically produced from ammonium fumarate in the presence of the enzyme, aspartase. A cell-free aspartase solution is prepared by forming an aqueous cell suspension of aspartase- containing microbial cells; incubating the cell suspension, centrifuging the cell suspension to form a clarified solution that contains a major portion of the aspartase activity originally present in the cells, a sufficient amount of an aspartase-stabilizing salt being included in the cell suspension or to the clarified solution, to make the clarified solution hypertonic. Aspartase recovered in this manner is particularly useful in immobilized enzyme reactors for the production of aspartic acid.

Improvements in Methods and Apparatus for Manufacturing Enzymes and Toxines by Aerobic Bacteria.

... 16,198. Boidin, A., and Effront, J. July 11, 1913, [Convention date]. Diastase.-Enzymes and toxines are prepared by cultivating aerobic bacteria in contact with air in thin layers of a mash rich in nitrogenous constituents and poor in carbohydrates, formed of vegetable or animal albuminoid materials, such as casein, fibrin of maize, soy-cake, from which the fat has been extracted, or bran or other vegetable waste product which has been freed from caxbohydrates by saccharification, fermentation, and distillation. The mash prepared in a boiler 1 is forced by air pressure into a sterilized culture chamber 15 formed of materials, such as aluminium, enamelled metal, porcelain, or glass, which do not affect bacteria. The mash is distributed in thin layers on trays 25, mounted on a central shaft which is rotated either with a to-and-fro motion to agitate the mash or rapidly to discharge the trays by oentrifugal action. Either a separate culture is added to the mash, or portions of a previous culture ...

FERMENTATION PROCEDURE WITH CYCLIC PULSE INTERRUPTION FEED

FERMENTATION PROCESSES USING SCRAPED TUBULAR FERMENTOR

FERMENTATION PROCESSES USING SCRAPED TUBULAR FERMENTOR Fermentation of a substrate by microorganisms capable of utilizing the substrate for growth is improved by conveying a fermentation medium including the substrate and microoganisms through a hollow tube with the assistance of an internal wall-scraper.

METHODS AND SYSTEMS FOR OPTIMIZING PERFUSION CELL CULTURE SYSTEM

Methods and perfusion culture systems are disclosed. The systems and methods relate to decreasing the starting perfusion rate, resulting in increased residence time of the cells in the bioreactor and the cell retention device, and/or concomitantly increasing the starting bioreactor volume or decreasing the starting cell retention device volume, or both. Other method embodiments include increasing the concentrations of individual components of the tissue culture fluid, and adding a stabilizer of the degradation of the recombinant protein.

Improved raw material recycling and separating system for urokinase pharmaceutical production

Enzyme preparation preparation device for organic synthesis

PRODUCTION OF ASPARTASE

A METHOD FOR PRODUCING A PRODUCT (E.G. POLYPEPTIDE) IN A CONTINUOUS CELL CULTURE FERMENTATION PROCESS.

Amethod for improving productivity in microbial fermentations and mammalian cell culture bioreactors.

METHOD FOR PRODUCING CELLULASE, AND APPARATUS FOR SAID METHOD

A method for producing a cellulase, comprising steps (1) to (3) mentioned below, wherein the resultant cellulase can be used effectively in washing agents and for the hydrolysis of starches, the hydrolysis of celluloses and so on: (1) filtrating an aqueous solution of a cellulase originated from a filamentous fungus through an ultrafiltration membrane having a cut-off molecular weight of 100,000 to 200,000 to produce a filtrate and a concentrated enzyme solution that is a non-filtrate; (2) filtering the filtrate produced in step (1) through a second ultrafiltration membrane having a cut-off molecular weight of 5,000 to 50,000 to produce a second concentrated enzyme solution that is a non-filtrate; and (3) mixing the concentrated enzyme solutions produced in steps (1) and (2) together to produce a cellulase originated from the filamentous fungus.

DIRECTED SELECTIVE SOLID-PHASE CULTURING OF STABLE MICROBIAL MIXED POPULATIONS FOR THE CONTINUOUS PREPARATION OF DEFINED ENZYME AND METABOLITE MIXTURE AND BIOREACTOR

The present invention provides a method for directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of deflned enzyme and metabolite mixtures, enzyme and metabolite materials obtained by such method and suitable devices therefore.

APPARATUS AND METHOD FOR DEHYDRATING BIOLOGICAL MATERIALS

An apparatus and method for microwave vacuum-drying of temperature- sensitive biological materials on a continuous flow-through basis, in which the materials are frozen, ground to frozen particles, dehydrated to a powder, and the powder collected. The apparatus (10) has a microwave generator (12) and waveguide (14), a freezing chamber (46) with a grinder (52), a rotatable dehydration chamber (18) in or adjacent to the waveguide, and a powder collector (82) to receive the powdered biological material. The apparatus operates under reduced pressure provided by a vacuum system (92) coupled to the powder collector (82).

PROCESS FOR THE PRODUCTION OF LIGNOLYTICAL ENZYMES BY MEANS OF PHANEROCHAETE CHRYSOSPORIUM

Summary The present invention concerns a process for the production of lignolytical enzymes by means of Phanerochaete chrysosporium wherein a) a culture of the pocket rot fungus Phanerochaete chrysosporium is placed into a closed vessel (1) which has no stirring means, b) pellets of Phanerochaete chrysosporium are produced there by rotating and slewing said vessel (1), and c) enzyme is then harvested. Intended for publication: figure ...

A novel urokinase drug used in the production of the raw material recovery and separation system

PROCEDE DE PREPARATION D'UNE SOLUTION AQUEUSE, PRATIQUEMENT DEPOURVUE DE CELLULES, D'ASPARTASE ET SON APPLICATION A LA FABRICATION DE L'ACIDE ASPARTIQUE

ON PRODUIT DE L'ACIDE ASPARTIQUE, PAR VOIE ENZYMATIQUE, A PARTIR DE FUMARATE D'AMMONIUM EN PRESENCE D'UNE ENZYME, L'ASPARTASE. ON PREPARE UNE SOLUTION D'ASPARTASE DEPOURVUE DE CELLULES EN FORMANT UNE SUSPENSION AQUEUSE DE CELLULES MICROBIENNES CONTENANT DE L'ASPARTASE, EN FAISANT INCUBER CETTE SUSPENSION DE CELLULES, EN CENTRIFUGEANT LA SUSPENSION DE CELLULES POUR FORMER UNE SOLUTION CLARIFIEE QUI RENFERME LA MAJEURE PARTIE DE L'ACTIVITE ASPARTASE PRESENTE A L'ORIGINE DANS LES CELLULES, ET EN AJOUTANT A LA SUSPENSION DE CELLULES OU A LA SOLUTION CLARIFIEE UNE QUANTITE SUFFISANTE D'UN SEL STABILISATEUR DE L'ASPARTASE POUR RENDRE HYPERTONIQUE LA SOLUTION CLARIFIEE. L'ASPARTASE RECUEILLIE DE CETTE MANIERE EST PARTICULIEREMENT UTILE DANS LES REACTEURS A ENZYMES IMMOBILISES POUR LA FABRICATION DE L'ACIDE ASPARTIQUE.

SYSTEM, APPARATUS AND METHOD FOR THE PRODUCTION OF BIOMOLECULES

METHODS AND SYSTEMS FOR OPTIMIZING PERFUSION CELL CULTURE SYSTEM

Methods and perfusion culture systems are disclosed. The systems and methods relate to decreasing the starting perfusion rate, resulting in increased residence time of the cells in the bioreactor and the cell retention device, and/or concomitantly increasing the starting bioreactor volume or decreasing the starting cell retention device volume, or both. Other method embodiments include increasing the concentrations of individual components of the tissue culture fluid, and adding a stabilizer of the degradation of the recombinant protein.

VERFAHREN UND VORRICHTUNG ZUR HERSTELLUNG VON ENZYMEN MIT EINEM REKOMBINANTE PLASMIDE ENTHALTENDEN BAKTERIUM

The process comprises culturing a host bacterium into which enzyme genes and a control gene having an apo-repressor have been introduced, so that formation of enzyme is repressed while the bacterium grows in a culture medium containing a co-repressor, removing the co-repressor from the culture solution and subsequently allowing the host bacterium to produce the enzyme in the culture medium containing no co-repressor. An apparatus for producing an enzyme includes a fermentor 1 a cylindrical filter 11 for removing the co-repressor from circulating culture solution from the fermentor, and a culture medium reservoir 26 for feeding fresh culture medium containing no co-repressor into the fermentor 1. ...

Multicomponent solid phase-submerged culture system, useful for preparation and testing of high performance strains for e.g. enzyme production or composting

Solid phase/submerged multicomponent culture system (A) and corresponding culture method. Solid phase/submerged multicomponent culture system (A) and corresponding culture method comprises: (i) one or more trickle bed/fluidized bed solid-state fermenters with, additionally in the trickle bed, stirrers that, before switching to fluidized bed mode, homogenize the mycelium formed (or the mycelial coating), either stepwise or, for fungi, continuosly, e.g to inhibit undesirable sporulation of strongly sporulating fungi; and (ii) one or more submerged fermenters in which, either with or after milling (using an Ultra-Turrax) of the mycelium transferred to them (after switching from trickle bed to to fluidized bed mode in the solid-substrate culture), the mycelium is cultured in batch or (semi) continuous mode. To generate stable, high capacity strains, the submerged fermenter comprises a quartz tube and closure plate (with standard fittings) and also includes a UV source and one or more solid-state ...

Improvements in and relating to the production of bacterial enzyme preparations and the cultivation of micro-organism of industrial and nutritional value

... 758,811. Bacteria, cultivating. LYONS & CO., Ltd., J., and CLAYSON, D. H. F. Oct. 30, 1951 [Oct. 30, 1950], No. 26402/50. Class 81 ( 1 ). In the production of enzymes of microbiological origin, microbes are introduced into a solid or semi-solid culture medium made absorbent by means of a distending agent, and the medium is maintained in a condition for producing their growth. The culture medium may be prepared by making a dough or batter of flour(s), a nutrient and a distending agent e.g. yeast, which also acts as the nutrient, or baking powder or ammonium carbonate. The medium, which may be heat-sterilized, may also contain materials such as glass wool, asbestos, cellulosic material. Inorganic nutrients, e.g. nitrates may be present. A suitable apparatus consists of a casing a in which is disposed an endless open wirework conveyer f carrying the dough. Air moistened by bubbling through heated water in vessel i is passed into the chamber through pipe g.

Enzyme preparation preparing device for organic synthesis

Constant-temperature shaking incubator

Finished product residual liquid recoverable post-extraction production line of enzyme preparation fermentation liquid

APPARATUS FOR PREPARING ENZYME-IMMOBILIZED BEADS AND METHOD FOR PREPARING ENZYME-IMMOBILIZED BEADS USING SAME

The present invention relates to an apparatus for preparing enzyme-immobilized beads used for preparation of tagatose, and a method for preparing enzyme-immobilized beads using the same. More specifically, the present invention relates to an apparatus for preparing enzyme-immobilized beads, comprising a nozzle with an inside diameter of 0.1-1 mm having a cylindrical lower end and comprising a cut-type liquid outlet (cut perpendicularly to the vertical axis of the nozzle) formed at the lower end, and a method for preparing enzyme-immobilized beads using the same.

METHOD AND APPARATUS FOR TREATING MICROBIAL CELL AND MICROBIAL CELL CATALYST

PROBLEM TO BE SOLVED: To control reduction in enzyme activity in a microbial cell after completion of culture. SOLUTION: The method for treating a microbial cell comprises a culture process for culturing a microbial cell capable of producing an enzyme to give a microbial cell solution, a cooling process for cooling the microbial cell solution after culture and a separation process for separating the microbial cell from the cooled microbial cell solution. COPYRIGHT: (C)2006,JPO&NCIPI ...

PRODUCTION OF ASPARTASE FROM MICROORGANISMS AND SYNTHESIS OF ASPARTATE

Method for producing cellulase, and apparatus for said method

A method for producing a cellulase, comprising steps (1) to (3) mentioned below, wherein the resultant cellulase can be used effectively in washing agents and for the hydrolysis of starches, the hydrolysis of celluloses and so on: (1) filtrating an aqueous solution of a cellulase originated from a filamentous fungus through an ultrafiltration membrane having a cut-off molecular weight of 100,000 to 200,000 to produce a filtrate and a concentrated enzyme solution that is a non-filtrate; (2) filtering the filtrate produced in step (1) through a second ultrafiltration membrane having a cut-off molecular weight of 5,000 to 50,000 to produce a second concentrated enzyme solution that is a non-filtrate; and (3) mixing the concentrated enzyme solutions produced in steps (1) and (2) together to produce a cellulase originated from the filamentous fungus.

METHOD FOR PRODUCING CELLULASE AND APPARATUS FOR SAID METHOD

A method for producing a cellulase, comprising steps (1) to (3) mentioned below, wherein the resultant cellulase can be used effectively in washing agents and for the hydrolysis of starches, the hydrolysis of celluloses and so on: (1) filtrating an aqueous solution of a cellulase originated from a filamentous fungus through an ultrafiltration membrane having a cut-off molecular weight of 100,000 to 200,000 to produce a filtrate and a concentrated enzyme solution that is a non-filtrate; (2) filtering the filtrate produced in step (1) through a second ultrafiltration membrane having a cut-off molecular weight of 5,000 to 50,000 to produce a second concentrated enzyme solution that is a non-filtrate; and (3) mixing the concentrated enzyme solutions produced in steps (1) and (2) together to produce a cellulase originated from the filamentous fungus.

APPARATUS FOR CONVERTING INTO ADENOSINE-5'- TRIPHOSPHATE

ABSTRACT OF THE DISCLOSURE An apparatus for converting into ATP which comprises an enzyme reactor, a source of AMP supply, a source of phosphoric acid donator supply, variable fluid sending apparatus, an automatic sampling apparatus and an analyzing apparatus for the reacting solution, an arithmetic control apparatus, and a recovery apparatus. According to this apparatus, conversion from AMP or ADP into ATP can be effectively carried out and ATP conver-sion can be kept at substantial 100% over a long period of time. The device makes it possible for ATP to be used more and more in future as an energy source for bioreactors and as medicines because the ATP will be more readily available and less expensive.

FERMENTOR EQUIPPED WITH EJECTOR

Se describe un fermentador que comprende uno o más inyectores de dos fases para abastecer de oxígeno para la fermentación y un bucle de circulación, que hace circular el caldo de fermentación y que suministra líquido para los inyectores de dos fases. Se describe asimismo una planta de fermentación que comprende uno o más fermentadores de la presente y servicios para los fermentadores. También se da a conocer el uso de los fermentadores para la producción de un producto de la fermentación tal como una enzima.

Fermentation with cyclic pulse-pause feeding

A process for the production of a valuable compound, comprising the steps of a) fermentation of a filamentous bacterial or fungal strain (e.g. a Streptomyces strain or an Aspergillus strain) in a fermentation medium wherein a carbohydrate during fermentation is added in a cyclic pulse dosing/pause way, wherein the pulse dosing time is shorter than the pause time and b) recovery of the valuable compound from the fermentation broth.

VERFAHREN UND ANLAGE ZUR KONTINUIERLICHEN HERSTELLUNG VON FRUCTOSE-1,6-DIPHOSPHAT UNTER VERWENDUNG VON IMMOBILISIERTER HEFE

CULTURE APPARATUS

PURPOSE:To produce a large amount of the titled enzyme by device control, by cultivating a specific host mold in a medium containing a co-repressor (CRP) while suppressing enzyme formation, removing CRP from the culture solution and carrying out culture in a medium containing no CRP. CONSTITUTION:A host mold (e.g. Escherichia coli C600) transduced with an enzyme gene and an adjusting gene to form an aporepressor is cultivated in a medium containing a need amino acid, glucose and CRP (e.g. tryptophan in 100-200mug/ml concentration in a culture tank 1 at pH about 7 at about 37 deg.C for about 5hr to give a culture solution 12. Then the culture solution is sent through a piping 4, a pump 5 and a flowmeter 8 to a ceramic filter 11 to remove CRP and a prepared filtrate 12 is put in a filtrate tank 15. On the other hand, a mold-containing concentrated solution is circulated through a ball valve 16 and an outlet piping 17 to the culture tank 1. Then a medium containing no CRP is fed from a medium ...

Methods and systems for optimizing perfusion cell culture system

Methods and perfusion culture systems are disclosed. The systems and methods relate to decreasing the starting perfusion rate, resulting in increased residence time of the cells in the bioreactor and the cell retention device, and/or concomitantly increasing the starting bioreactor volume or decreasing the starting cell retention device volume, or both. Other method embodiments include increasing the concentrations of individual components of the tissue culture fluid, and adding a stabilizer of the degradation of the recombinant protein.

MULTIVARIATE SPECTRAL ANALYSIS AND MONITORING OF BIOMANUFACTURING

The disclosure features methods that include obtaining a vibrational spectrum (804) of a solution in a biological manufacturing system, analyzing the vibrational spectrum (806) using a first chemometrics model to determine a value of a first quality attribute associated with the solution, analyzing the vibrational spectrum (806) using a second chemometrics model to determine a value of a second quality attribute (812) associated with the solution, and adjusting at least one parameter (810) of a purification unit of the biological manufacturing system based on at least one of the values of the first and second quality attributes.

MICROFLUIDIC BIOREACTOR WITH MODULAR DESIGN FOR SYNTHESIZING CELL METABOLITES, METHOD FOR USING SAME, AND USE THEREOF

The invention relates to a microfluidic bioreactor (1) for obtaining cell metabolites, consisting of at least two modules (2, 3, 4, 5, 6, X) with a cavity that is separated by a membrane (20) into a cell chamber (211, 311, 411) for receiving cells and a material chamber (221, 321, 421) through which a liquid solution can flow, said membrane (20) being at least partly permeable, wherein the material chambers (221, 321, 421) are connected in series and/or in a parallel manner by a fluid conducting system (16). The invention likewise relates to the use of the microfluidic bioreactor according to the invention and to a method for obtaining cell metabolites.

Continuous production device for enzyme culture

本实用新型涉及一种酶培养连续生产装置，包括用于酶培养基的消毒罐和酶培养箱，其主要特点是：消毒罐的罐体由内外壁之间的间隙构成与给水管及排水管连通的冷却通道，消毒罐的顶部通过输料管与给料机连通，底部通过输料管与酶培养箱的进料口连通，在消毒罐的底部连通有加热输汽管。酶培养箱具有一个箱体，箱体内上下排列设置有多层酶培养移动机构，在箱体的顶部设有通过输气管与其连通的冷、热双温交换器和鼓风机。该装置能够实现酶培养的连续生产，大幅度的提高生产效率，并能可靠地保证酶的产品质量，满足规模化连续生产的要求。

MANUFACTURING DEVICE OF ENZYME-IMMOBILIZED BEADS AND MANUFACTURING METHOD FOR ENZYME-IMMOBILIZED BEADS USING THE DEVICE

METHOD FOR PRODUCING CELLULASE, AND APPARATUS FOR SAID METHOD

Multiphase reactor systems based on foams for simultaneous growth and separation of products

A nutrient gas is introduced into a liquid nutrient formulation. The resulting nutrient media is a foam. The bubble size of the gas phase is between 0.1 to 200 microns and the foam is 40-80% voids.

Microscale bioprocessing system and method for protein manufacturing

A bioprocessing system for protein manufacturing is provided that is compact, integrated and suited for on-demand production and delivery of therapeutic proteins to patients. The system can also be used for efficient on-demand production of any type of protein.

Apparatus for preparing immobilized-enzyme beads and method for preparing immobilized-enzyme beads using same

The present invention relates to an apparatus for preparing enzyme-immobilized beads used for preparation of tagatose, and a method for preparing enzyme-immobilized beads using the same. More specifically, the present invention relates to an apparatus for preparing enzyme-immobilized beads, comprising a nozzle with an inside diameter of 0.1-1 mm having a cylindrical lower end and comprising a cut-type liquid outlet (cut perpendicularly to the vertical axis of the nozzle) formed at the lower end, and a method for preparing enzyme-immobilized beads using the same.

A METHOD FOR PRODUCING A PRODUCT (E.G. POLYPEPTIDE) IN A CONTINUOUS CELL CULTURE FERMENTATION PROCESS

A method for improving productivity in microbial fermentations and mammalian cell culture bioreactors. 114-. (canceled)16. The method of claim 15 , wherein the product is selected from a biopolymer claim 15 , such as a polypeptide or protein.17. The method of claim 15 , wherein the product is selected from an intracellular product claim 15 , such as an inclusion body or from a periplasmatic product claim 15 , such as a precipitated product in solid form such as in crystalline claim 15 , amorphous or denatured form.18. The method of claim 15 , wherein the product is selected 5 from a microorganism claim 15 , such as yeast claim 15 , virus and bacteria or from a cell claim 15 , such as a mammalian cell.19. The method of claim 15 , wherein the cell density in the bioreactor during the fermentation reaches at least 10 million cells per ml medium such as at least 20 million cells per ml medium such as at least 30 million cells per ml medium.20. The method of claim 15 , wherein impurities are removed via the separation device (e.g. the impurity filter unit) by one flow rate through the separation device (e.g. impurity filter unit) and the product is harvested through the product harvest module by a second flow rate through the product harvest module claim 15 , wherein the ratio between the first flow rate and the second flow rate is from 1:1 to 9:1.21. The method of claim 15 , wherein the separation device is selected from an impurity filter unit claim 15 , such as a membrane filter or a gravitational separation unit or a centrifugal separation unit.22. The method of claim 15 , wherein the bioreactor has a volume of at least 50 L claim 15 , such as at least 500 L.23. The method of claim 15 , wherein the impurity filter unit is a membrane filter having a nominal molecular weight cut-off (NMWC) pore sizes of at least 1000 NMWC claim 15 , such as within the range of 2000 to 15.000 NMWC.24. The method of claim 15 , wherein the impurity filter unit is a membrane filter having ...

Mikrofluidischer Bioreaktor mit modularem Aufbau zur Synthese von Zellmetaboliten, das Verfahren zur Nutzung sowie dessen Verwendung

Die vorliegende Erfindung betrifft einen mikrofluidischer Bioreaktor (1) zur Gewinnung von Zellmetaboliten, aus mindestens zwei Modulen (2, 3, 4, 5, 6, X) mit einer Kavität, die mit einer Membran (20) in eine Zellkammer (211, 311, 411) für die Aufnahmen von Zellen und einer mit einer flüssigen Lösung durchströmbaren Stoffkammer (221, 321, 421) unterteilt ist, wobei die Membran (20) teilweise durchlässig ist. Die Stoffkammern (221, 321, 421) werden durch ein Fluidleitungssystem (16) in Reihe und/oder parallel miteinander verbunden. Die Erfindung beschreibt ebenfalls die Verwendung des erfindungsgemäßen mikrofluidischen Bioreaktors sowie ein Verfahren zur Gewinnung von Zellmetaboliten.

VERFAHREN ZUR GEWINNUNG VON CELLULASE UND ANLAGE ZUR DURCHFUEHRUNG DES VERFAHRENS

ENZYME PRODUCTION

METHOD OF FERMENTATION USING TUBULAR FERMENTORS INCORPORATING WALL SCRAPERS

... 1499410 Fermentations in tubular fermenters incorporating wall-scrapers UNIVERSITY OF WATERLOO 15 March 1976 [6 May 1975] 19044/75 Heading C6F [Also in Division B1] A method of fermentation of a substrate by micro-organisms capable of utilizing the substrate for growth involves continuously conveying a fermentation medium including the substrate and micro-organisms through a hollow tube, fermenting the substrate by the micro-organisms during the conveying of the fermentation medium through the tube, and scraping the internal walls of the tube during the conveying of the fermentation medium through the tube. Referring to the drawings, the scraper may be in the form of helical coils 24, 30, Fig. 1, a helical ribbon, auger or screw 100, Figs. 2 and 3, or disc-plates (200) driven around a tubular circuit by flexible cables (201), Figs. 4 and 5 (not shown). In the Fig. 1 apparatus oxygencontaining gas (or nitrogen) is introduced into the fermentation medium through perforations 26 in the coil ...

ENZYME PRODUCTION PROCESS AND APPARATUS

Process and apparatuses for the manufacture of the diastases and toxins by the oxidizing leavens

GENERATING PROCESS AND STATION UNINTERRUPTED OF FRUCTOSE-1,6-DIPHOSPHATE BY MEANS OF YEAST IMMOBILISEE

L'INVENTION CONCERNE UN PROCEDE DE PHOSPHORYLATION ENZYMATIQUE DE GLUCOSE EN FDP AU MOYEN DE LEVURE IMMOBILISEE AU GLUTARALDEHYDE ET LA SEPARATION DU FDP PAR ULTRAFILTRATION. ON INTRODUIT UNE SUSPENSION DE LEVURE TRAITEE AU GLUTARALDEHYDE DANS UN FERMENTEUR1, EN AJOUTANT UN MELANGE NUTRITIF CONSTITUE PAR DU DEXTROSE (1M), DU PHOSPHATE DE SODIUM (0,5), DU CHLORURE DE MAGNESIUM (10MM) ET DE L'ACIDE PHOSPHORIQUE JUSQU'A PH 6,5. ON RECYCLE LE MELANGE A TRAVERS UNE UNITE D'ULTRAFILTRATION A FIBRES CREUSES3 POUR SEPARER LE FDP QUI SE FORME PENDANT LA REACTION DE PHOSPHORYLATION. THE INVENTION CONCERNS A PROCESS FOR THE ENZYMATIC PHOSPHORYLATION OF GLUCOSE TO FDP BY MEANS OF YEAST IMMOBILIZED TO GLUTARALDEHYDE AND THE SEPARATION OF FDP BY ULTRAFILTRATION. A SUSPENSION OF YEAST TREATED WITH GLUTARALDEHYDE IS INTRODUCED IN A FERMENTER1, BY ADDING A NUTRITIONAL MIX CONSTITUTE OF DEXTROSE (1M), SODIUM PHOSPHATE (0.5), MAGNESIUM CHLORIDE (10MM) AND PHOSPHORIC ACID 'A PH 6.5. THE MIXTURE IS RECYCLED THROUGH A HOLLOW-FIBER ULTRAFILTRATION UNIT3 TO SEPARATE THE FDP WHICH FORMED DURING THE PHOSPHORYLATION REACTION.

Methods and systems for optimizing perfusion cell culture system

Methods and perfusion culture systems are disclosed. The systems and methods relate to decreasing the starting perfusion rate, resulting in increased residence time of the cells in the bioreactor and the cell retention device, and/or concomitantly increasing the starting bioreactor volume or decreasing the starting cell retention device volume, or both. Other method embodiments include increasing the concentrations of individual components of the tissue culture fluid, and adding a stabilizer of the degradation of the recombinant protein.

Directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of defined enzyme and metabolite mixtures

The present invention provides a method for directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of defined enzyme and metabolite mixtures, enzyme and metabolite materials obtained by such method and suitable devices therefore.

Bioreactor for production of products with immobilized biofilm

A bioreactor is provided for production of products by biosynthesis or biotransformation using a biofilm of immobilized cells. The bioreactor includes a horizontal cylindrical housing and a central rotatable shaft which extends along the axis of the housing. Connected to the central shaft are one or more screens which are oriented parallel to the shaft, with a small gap between the shaft and the screen. The bioreactor further includes a slidable blade which is mounted onto the shaft, through a set of poles, and which is located at a fixed distance from the screen so that the blade is made to pass over the screen by the force of gravity as the shaft rotates, as by the action of a rotating external magnet. An example of use of the bioreactor is the production of Υ-decalactone from castor oil using Tyromyces sambuceus.

Producing defined enzyme mixture and/or metabolite mixture for fermentation of target substrates, by inoculating microorganisms in solid-phase bioreactor with inducer substrates and keeping mixed culture under appropriate conditions

A culture method for producing a defined enzyme mixture and/or metabolite mixture optimized for the fermentation of one or more target substrates comprising contacting an inoculating mixed culture of microorganisms in a solid-phase bioreactor with target substrates, inducer substrates or any combination of target and inducer substrates, and by keeping the mixed culture under an appropriate conditions, is new. A culture method (M1) for producing a defined enzyme mixture and/or metabolite mixture optimized for the fermentation of one or more target substrates, by contacting an inoculating mixed culture of microorganisms in a solid-phase bioreactor with one or more target substrates, one or more inducer substrates or any combination of target and inducer substrates, and by keeping the mixed culture under an appropriate selection pressure by a suitable selection of the culturing parameters and by a specific induction by the target and/or inducer substrate and/or inhibition with appropriate ...

Process for the production of lignolytic enzymes by means of phanerochaete chrysosporium

FERMENTATION WITH CYCLIC PULSE-PAUSE FEEDING

A process for the production of a valuable compound, comprising the steps of a) fermentation of a filamentous bacterial or fungal strain (e.g. a Streptomyces strain or an Aspergillus strain) in a fermentation medium wherein a carbohydrate during fermentation is added in a cyclic pulse dosing/pause way, wherein the pulse dosing time is shorter than the pause time and b) recovery of the valuable compound from the fermentation broth.

photobioreactor

Photobioreactor

A method for non-invasive growth measurement of organisms in an apparatus for growing and harvesting organisms or substances derived from such organisms. The organisms are comprised in an aqueous medium in a vessel, the method comprising the steps of: a) Providing means for measuring the opacity of the aqueous medium in order to estimate a growth rate of the organisms; b) The means for measuring the opacity are arranged in the apparatus such that they are located at predetermined locations relative to the vessel; c) The means for measuring the opacity are calibrated to account for refraction, absorbance and reflection caused by the vessel at the predetermined location. Finally the method also allows for measuring the opacity of the aqueous medium at the predetermined location.

Animal enzyme preparation pepsin production plant

Photobioreactor

Perfusion bioreactor and related methods of use

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

PRODUCTION OF ASPARTASE

PROCESS FOR ENZYME PRODUCTION WITH BACTERIUM HARBORING RECOMBINANT PLASMIDS.

SYSTEM APPARATUS AND METHOD FOR PRODUCING BIOMOLECULES

BIOREACTOR FOR OBTAINING SECONDARY PLANT CONSTITUENTS

A bioreactor 1 for obtaining secondary plant constituents is presented. This consists of a flexible shell 2 in the form of a bag, closed at the top by a dimensionally stable lid 10 and at the bottom by a dimensionally stable base 20. In the interior, within the shell 2, at least one fixed carrier plate 30 is arranged in a freely suspended manner by means of a mounting assembly 31 secured to the lid (10). The bioreactor 1 can be charged with nutrients and gases via spray nozzles 11 in the lid 10. An outlet 21 in the base 20 is provided for the outflow of the remaining nutrient liquid and the desired metabolic product. The bioreactor 1 can be folded up together with the entire contents.

Perfusion bioreactor and related methods of use

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

METHOD FOR CONTROLLING ENZYME PRODUCTIVITY OF MICROORGANISMS

An object of the present invention is to provide a novel method for controlling enzyme productivity of a microorganism. A pulsed electric field is applied to a microorganism to control the enzyme productivity of the microorganism. 1. A method for controlling the enzyme productivity of a microorganism characterized by applying a pulsed electric field to a microorganism.2. The method according to claim 1 , comprising a step of applying the pulsed electric field to the culture solution during culture of the microorganism.3. The method according to claim 2 , wherein the culture solution circulates in an electrode part that generates the pulsed electric field during culture.4. The method according to claim 2 , wherein the pulsed electric field is repeatedly applied during culture.5. The method according to claim 1 , wherein a pulse waveform of the pulsed electric field is a damped oscillation waveform.6. The method according to claim 1 , wherein a field strength of the pulsed electric field is 10 kV/cm to 50 kV/cm.7. The method according to claim 1 , wherein a production amount of one or more enzymes selected from the group consisting of amylase claim 1 , glucosidase claim 1 , galactosidase claim 1 , cellulase claim 1 , esterase claim 1 , lipase claim 1 , protease claim 1 , phosphatase claim 1 , peptidase claim 1 , nuclease claim 1 , deaminase claim 1 , oxidase claim 1 , dehydrogenase claim 1 , glutaminase claim 1 , pectinase claim 1 , catalase claim 1 , dextranase claim 1 , transglutaminase claim 1 , protein deamidase claim 1 , and pullulanase is controlled.8. The method according to claim 1 , wherein a production amount of one or more enzymes selected from the group consisting of α-amylase claim 1 , α-glucosidase claim 1 , β-glucosidase claim 1 , α-galactosidase claim 1 , β-galactosidase claim 1 , cellulase claim 1 , esterase claim 1 , lipase claim 1 , protease claim 1 , acid phosphatase claim 1 , alkaline phosphatase claim 1 , leucine peptidase claim 1 , alanine ...

Multivariate Spectral Analysis and Monitoring for Biomanufacturing

The disclosure features methods that include obtaining a vibrational spectrum of a solution in a biological manufacturing system, analyzing the vibrational spectrum using a first chemometrics model to determine a value of a first quality attribute associated with the solution, analyzing the vibrational spectrum using a second chemometrics model to determine a value of a second quality attribute associated with the solution, and adjusting at least one parameter of a purification unit of the biological manufacturing system based on at least one of the values of the first and second quality attributes.

BIOREACTOR AND USES THEREOF

The present invention relates to the field of fermentation. In particular, the present invention relates to a method and apparatus for production of products by use of living cells or active components derived from such cells using a bioreactor having a built in gas distributor and a specific system/device for handling foam formed in the process. The invention also relates to a bioreactor comprising: a reaction chamber having a fermentation zone and a foam settling zone, wherein the reaction chamber is arranged such that the foam settling zone is spaced from or physically separated from the fermentation zone so as to reduce the effect of activity in the fermentation zone on foam in the settling zone. The separation of the fermentation zone from the activity of the fermentation zone encourages settling of the foam. The invention also provides a method of forming a bioreactor comprising: overlapping two flat, flexible sheets of material; and causing said two sheets to adhere to each other in selected areas so as to define a reaction chamber. This is a particularly cost-effective way to produce a single use, disposable reactor. 1. A bioreactor comprising:a reaction chamber having a fermentation zone and a foam settling zone, wherein the reaction chamber is arranged such that the foam settling zone is spaced from or physically separated from the fermentation zone so as to reduce the effect of activity in the fermentation zone on foam in the settling zone.2. The bioreactor as claimed in claim 1 , wherein the foam settling zone is formed as a laterally enlarged section of the reaction chamber.3. The bioreactor as claimed in claim 2 , wherein the foam settling zone is positioned to one side of the fermentation zone.4. The bioreactor as claimed in claim 1 , wherein the foam settling zone has a bottom wall that slopes downwardly towards the fermentation zone so that settled foam can flow as liquid back to the fermentation zone under gravity.5. The bioreactor as claimed in ...

APPARATUS AND METHOD FOR DEHYDRATING BIOLOGICAL MATERIALS

An apparatus and method for microwave vacuum-drying of temperature-sensitive biological materials on a continuous flow-through basis, in which the materials are frozen, ground to frozen particles, dehydrated to a powder, and the powder collected. The apparatus has a microwave generator and waveguide, a freezing chamber with a grinder, a rotatable dehydration chamber in or adjacent to the waveguide, and a powder collector to receive the powdered biological material. The apparatus operates under reduced pressure provided by a vacuum system coupled to the powder collector. 1. An apparatus for continuous throughput dehydration of an aqueous biological material , comprising:(a) a microwave generator;(b) a waveguide to direct microwave radiation from the generator;(c) a freezing chamber for receiving the aqueous biological material and freezing it to form a frozen aqueous biological material;(d) means for feeding the aqueous biological material into the freezing chamber;(e) means for forming a particulate frozen aqueous biological material from the frozen aqueous biological material;(f) a rotatable dehydration chamber in fluid communication with the freezing chamber, the dehydration chamber being positioned in the waveguide to receive microwave radiation produced by the generator, the dehydration chamber having a wall that is transparent to microwave radiation;(g) means for rotating the dehydration chamber;(h) a powder collector in fluid communication with the dehydration chamber;(i) a vacuum system; and(j) means for operatively connecting the vacuum system to the powder collector for applying a vacuum to the freezing chamber, the dehydration chamber and the powder collector whereby the vacuum conveys dehydrated biological material from the dehydration chamber to the powder collector on a continuous throughput basis.2. An apparatus according to claim 1 , further comprising an agitator in the dehydration chamber.3. An apparatus according to claim 1 , further comprising ...

PERFUSION BIOREACTOR AND RELATED METHODS OF USE

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate. 1. A method of controlling a bioreactor system , comprising:providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI);measuring one or more process parameters (PPs) of the culture within the bioreactor by a RAMAN probe, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes;measuring a weight of the bioreactor with cell culture contents;removing cell-free spent media from the cell culture using a first output conduit at a first specified rate;removing cells from the cell culture using a second output conduit at a second specified rate;introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate; andwherein the input and output conduits are adjusted based on the RAMAN probe measurements and weight measurement of the bioreactor to maintain (i) one or more of the process parameters within predetermined ranges, (ii) the weight of the bioreactor with the cell culture within a predetermined range, and (iii) the third ...

Ejector Equipped Fermenter

Disclosed is a fermenter comprising one or more two phase injectors for providing oxygen for the fermentation and a circulation loop, circulating the fermentation broth and providing liquid for the two-phase injectors. Further disclosed is a fermentation plant comprising one or more fermenters of the invention and utility for the fermenters. Also disclosed is the use of the fermenters for the production of a fermentation product such as an enzyme. 116-. (canceled)17. A fermenter for fermentation of microorganisms for the production of a desired product , comprising a tank , one or more two-phase injectors for supplying oxygen localized in the lower part of the fermenter , at least one loop withdrawing fluid from the fermenter and circulating the fluid to provide liquid for the two-phase injectors , one or more inlets and one or more outlets.18. The fermenter of claim 17 , wherein at least one of the one or more two-phase injectors are arranged so the injected stream is injected at an angle to a horizontal plane of 10-80°.19. The fermenter of claim 18 , comprising at least two two-phase injectors claim 18 , where at least one injector provides an injected stream going down claim 18 , and at least one injector provides an injected stream going up.20. The fermenter of claim 17 , comprising at least 0.04 two-phase injector per mfermenter volume (=1 injector per 25 m).21. The fermenter of claim 17 , wherein the fermenter is capable of delivering at least 400 mg Oper hour per kg fermentation broth (mgO/kg/hr).22. The fermenter of claim 17 , wherein the circulation loop comprises one or more inlets and one or more outlets and is connected to a pump.23. The fermenter of claim 17 , further comprising one or more probes for measuring the conditions in the fermenter.24. The fermenter of claim 17 , comprising a volume of at least 50 liters.25. The fermenter of claim 17 , wherein the fermenter is sterilisable or CIP cleanable.26. A method for producing one or more fermentation ...

APPARATUS FOR PREPARING IMMOBILIZED-ENZYME BEADS AND METHOD FOR PREPARING IMMOBILIZED-ENZYME BEADS USING SAME

The present invention relates to an apparatus for preparing enzyme-immobilized beads used for preparation of tagatose, and a method for preparing enzyme-immobilized beads using the same. More specifically, the present invention relates to an apparatus for preparing enzyme-immobilized beads, comprising a nozzle with an inside diameter of 0.1-1 mm having a cylindrical lower end and comprising a cut-type liquid outlet (cut perpendicularly to the vertical axis of the nozzle) formed at the lower end, and a method for preparing enzyme-immobilized beads using the same. 1. A method for producing a sweetener , comprising:1) forming immobilized enzyme beads by introducing a mixed liquid containing an enzyme-containing material and an excipient into an immobilization apparatus, wherein the immobilization apparatus comprises:a) a first tank into which the mixed liquid containing the enzyme-containing material and the excipient is injected;b) a nozzle unit disposed at a lower end of the first tank and comprising a nozzle, the nozzle having an inner diameter of 0.1 mm to 1 mm and a cylindrical lower end and including a liquid outlet cut perpendicularly to a vertical axis of the nozzle at the lower end thereof; andc) a second tank disposed below the nozzle unit and containing a calcium chloride solution; wherein the distance between the surface of the calcium chloride solution and the liquid outlet of the nozzle unit ranges from 0.1 m to 1.5 m;{'b': '1', '2) adding the beads formed in step ) to a reactor column;'}3) transferring a solution containing a reactant for producing a sweetener to the reactor column;4) producing the sweetener from the reactant by performing a reaction catalyzed by the enzyme.2. The method according to , wherein the nozzle unit in the immobilization apparatus according to comprises at least two nozzles.3. The method according to claim 1 , wherein the first tank in the immobilization apparatus comprises an air inlet.4. The method according to claim 1 , ...

METHOD FOR PRODUCING A PRODUCT (E.G. POLYPEPTIDE) IN A CONTINUOUS CELL CULTURE FERMENTATION PROCESS

A method for improving productivity in microbial fermentations and mammalian cell culture bioreactors. 2. The method of claim 1 , wherein the product is selected from a biopolymer.3E. coli, Bacillus,Saccharomyces, Pichia, Aspergillus, Fusarium, Kluyveromyces,. The method of claim 2 , wherein the biopolymer is expressed by at least one cell selected from the group consisting of yeast from the genus of CHO (Chinese Hamster Ovary) cell claim 2 , hybridomas claim 2 , BHK (Baby Hamster Kidney) cell claim 2 , myeloma cell claim 2 , HEK-293 cell claim 2 , human lymphoblastoid cell claim 2 , a human cell and a mouse cell.4. The method of claim 2 , wherein the biopolymer is a polypeptide or protein.5. The method of claim 4 , wherein the polypeptide is an antibody or fragment thereof claim 4 , Human growth hormone claim 4 , Follicle-stimulating hormone claim 4 , Factor VIII claim 4 , Factor VII claim 4 , Factor IX Erythropoietin (EPO) claim 4 , Granulocyte colony-stimulating factor (G-CSF) claim 4 , alpha-glactosidase A claim 4 , alpha-L-iduronidase (rhIDU; laronidase) claim 4 , N-acetylgalactosamine-4-sulfatase (rhASB; galsulfase) claim 4 , DNAse claim 4 , Tissue plasminogen activator (TPA) claim 4 , Glucocerebrosidase claim 4 , Interferon (IF) claim 4 , Insulin claim 4 , Insulin derivative claim 4 , Insulin-like growth factor 1 claim 4 , Tenecteplase claim 4 , antihemophilic factor claim 4 , human coagulation factor claim 4 , Etanercept claim 4 , Trastuzumab claim 4 , Infliximab claim 4 , Basiliximab claim 4 , Belimumab claim 4 , Daclizumab claim 4 , Adalimumab Abciximab or claim 4 , Afutuzumab claim 4 , Alemtuzumab claim 4 , Cetuximab claim 4 , Daclizumab claim 4 , Denosumab claim 4 , Eculizumab claim 4 , Edrecolomab claim 4 , Golimumab claim 4 , Ibritumomab tiuxetan claim 4 , Mepolizumab claim 4 , Motavizumab claim 4 , Natalizumab claim 4 , Ofatumumab claim 4 , Omalizumab claim 4 , Oregovomab claim 4 , Palivizumab claim 4 , Pemtumomab claim 4 , Pertuzumab claim 4 , ...

Multivariate Spectral Analysis and Monitoring for Biomanufacturing

The disclosure features methods that include obtaining a vibrational spectrum of a solution in a biological manufacturing system, analyzing the vibrational spectrum using a first chemometrics model to determine a value of a first quality attribute associated with the solution, analyzing the vibrational spectrum using a second chemometrics model to determine a value of a second quality attribute associated with the solution, and adjusting at least one parameter of a purification unit of the biological manufacturing system based on at least one of the values of the first and second quality attributes. 1. A method , comprising:obtaining a vibrational spectrum of a solution in a biological manufacturing system;analyzing the vibrational spectrum using a first chemometrics model to determine a value of a first quality attribute associated with the solution;analyzing the vibrational spectrum using a second chemometrics model to determine a value of a second quality attribute associated with the solution; andadjusting at least one parameter of a purification unit of the biological manufacturing system based on at least one of the values of the first and second quality attributes.2. The method of claim 1 , further comprising using the biological manufacturing system to produce at least one of:a protein-based therapeutic substance comprising at least one of a protein, a peptide, an antibody, and an enzyme;a nucleic acid-based drug substance comprising at least one of DNA, a plasmid, an oligonucleotide, an aptamer, a DNAzyme, an RNA aptamer, an RNA decoy, a microRNA fragment, and a small interfering RNA fragment; anda gene therapy drug substance.3. The method of claim 1 , wherein the first and second quality attributes are each independently selected from the group consisting of product quality attributes claim 1 , product-related impurities claim 1 , and process-related impurities claim 1 , for a biological product produced by the biological manufacturing system.4. The method ...

System and method of producing glucomylases and/or proteases

The present invention is concerned with a system of production of glucoamylases and/or proteases. The system has a packed bed solid state fermentation bioreactor. The bioreactor is adapted to contain and to operate using organic waste as fermentation substrate. The bioreactor is provided with spray nozzle for controlling moisture content of the fermentation substrate, means for supplying air to pass through packed bed of the fermentation substrate in said bioreactor, means for analyzing gas content in said bioreactor, means for monitoring temperature in said bioreactor, means for controlling temperature of said bioreactor, a port via which a sample in said bioreactor is obtainable, and means for harvesting enzymatic solution from said bioreactor, the enzymatic solution containing the glucoamylases and/or proteases.

MICROSCALE BIOPROCESSING SYSTEM AND METHOD FOR PROTEIN MANUFACTURING

A bioprocessing system for protein manufacturing is provided that is compact, integrated and suited for on-demand production and delivery of therapeutic proteins to patients. The system can also be used for efficient on-demand production of any type of protein. 1. A bioprocessing system , comprising:a reactor for protein expression;a membrane chromatography component for receiving and purifying protein output by the reactor; anda diafiltration component for receiving purified protein from the membrane chromatography and for further purifying the purified protein.2. The system of claim 1 , wherein the reactor is adapted for cell-free protein expression.3. The system of claim 2 , wherein the reactor has a capacity of approximately 20 ml or less and the membrane chromatography component has a capacity of less than approximately 5 ml.4. The system of claim 1 , wherein the diafiltration component comprises:a product section for receiving purified protein from the membrane chromatography component; anda buffer section for receiving a buffer solution.5. The system of claim 4 , wherein the diafiltration component comprises:a first substrate;a second substrate; anda diafiltration membrane positioned between the first and second substrates.6. The system of claim 5 , wherein the product section comprises a serpentine-shaped channel formed on the first substrate and the buffer section comprises a serpentine-shaped channel formed on the second substrate claim 5 , wherein the first and second serpentine-shaped channels substantially overlap each other.7. The system of claim 4 , wherein the diafiltration component comprises a flow cell.8. The system of claim 7 , wherein the product section comprises tubing positioned within the flow cell and the diafiltration membrane comprises the tubing material.9. The system of claim 1 , wherein the reactor comprises at least one sensor for monitoring reaction parameters.10. The system of claim 9 , wherein the at least one sensor monitors at ...

器官和组织的脱细胞化和再细胞化

本发明提供了使实体器官脱细胞化和使这类脱细胞器官再细胞化成实体器官的方法和材料。

Multifunctional post-extraction production line for enzyme preparation fermentation liquid

本发明公开了一种多功能型酶制剂发酵液后提取生产线，提供了一套完整的提高酶制剂纯度、调控酶液活力、减少废水排放、实现无菌超滤浓缩、解决成品液残留等多项功能的酶制剂发酵液后提取生产线。该多功能型酶制剂发酵液后提取生产线主要特征在于把板框压滤、管式膜微滤、卷式膜超滤在处理固液分离、液液分离上的各自优势充分利用起来，通过各操作单元细节的结构设计，进行了完美的组合，在除菌、除渣、纯化有效成分、无菌浓缩、成品液残留回收、减少劳动强度、节能减排等方面取得了显著的效果。

Multiple-stage process and equipment for the conversion of organic and inorganic materials by catalysts

A cylindrical reactor vessel (1) is subdivided by further concentrically arranged cylinders into a plurality of stages, which, depending on the the direction of flow, have overruns at the top end or overflow ducts at the bottom end. When biogas is produced from highly organically pollutated waste waters, e.g., three stages are situated in the reactor through which flow passes successively. In the inner stage (6) the hydrolysis takes place, in the middle stage (7) the acid formation takes place and in the outer stage (8) the methane formation takes place. The untreated water is continuously introduced into the inner stage from below by a feed pipe (11). The preclarified water collects after passing through all stages in the annular duct (12) and is taken off via the connection branch (13). It is fed into the annular space (18) via the feed branches (23), where degassing takes place. The gases produced from the outlet orifices (9, 19) are fed jointly for suitable further processing. The degassed liquid passes into the sedimenter (30) where the entrained sludge is separated off and returned from there to the reactor. The clarified waste water collects in an annular duct (26) and is taken off via the connection branch (27). <IMAGE>

Process and apparatus for the production of an enzymatic biomass from sugar beet pulp

The process is suitable for obtaining enzymes and biogas and entails extracted sugar beet pulp being fermented with Aspergillus niger, especially Aspergillus niger Z 317. An apparatus for carrying out the process consists of a vertical container which is divided by horizontal partitions into an upper first growing zone, a middle second growing zone and a lower stirring zone. After the first growing zone, sugar beet pulp on which there is growth is removed as material for inoculating the feedstock. <IMAGE>

Multifunctional post-extraction production line for enzyme preparation fermentation liquid

本实用新型公开了一种多功能型酶制剂发酵液后提取生产线，其特征在于发酵液储罐的出口通过阀门、泵与板框压滤机、压滤清液储罐组成板框压滤系统；压滤清液储罐的出口通过阀门、泵与微滤膜过滤机组成管式微滤循环系统；微滤超滤后浓缩液储罐的出口通过阀门、泵与超滤膜过滤机、浓缩液储罐组成卷式超滤膜过滤系统。本实用新型把板框压滤、管式膜微滤、卷式膜超滤在处理固液分离、液液分离上的各自优势充分利用起来，通过各操作单元细节的结构设计，进行了完美的组合，在除菌、除渣、纯化有效成分、无菌浓缩、成品液残留回收、减少劳动强度、节能减排等方面取得了显著的效果。

Enzyme microb installation for fermenting

本发明公开一种微生物酵素发酵装置，包括一发酵槽单元、一热交换单元、一以纯水为媒介且能够循环调配的供料单元、一输出单元、一设有纳米过滤网和分子膜气爆管的供气单元，以及一控制前述各单元的控制单元。本发明具备综合性的完整功能，并且该供气单元配合真空机的汲吸排气作用，以气爆方式来搅拌发酵液体流动循环混合，能够避免伤害微生物菌种，以及让气体与发酵液体及微生物充分地混合接触，大幅提高微生物的生长及产率，能够用来生产优质的酵素或胜肽等产品，灵活地应用于生物食用酵素、植物叶面酵素及土壤改进剂等各种用途。

A kind of biological enzyme agitator

本发明公开了一种生物酶搅拌桶，包括转轴、闭合盖和搅拌桶本体，所述搅拌桶本体的下端固定安装有减震底座，减震底座的下端面通过减震弹簧连接有支撑底座，支撑底座上端面一侧固定安装有滑动连接杆，滑动连接杆的末端滑动安装在减震底座内，所述搅拌桶本体内安装有斜位下料板，所述闭合盖螺纹安装在搅拌桶本体的上端，闭合盖上端面一侧安装有进料口，进料口的一侧安装有电机，所述转轴转动安装在闭合盖的下端面，转轴的下端安装有安装柱，安装柱上开设有螺纹孔，螺纹孔内螺纹安装有螺纹轴，螺纹轴的末端分别安装有第一搅拌叶、第二搅拌叶和第三搅拌叶。本发明具有快速均匀搅拌且方便清洗和方便拆卸搅拌叶的优点。

System, apparatus and method for biomolecules production

The current invention concerns an automated method for the production of cells and/or biomolecules such as protein or peptides comprising the steps of culturing cells in at least one high cell density bioreactor, thereby fluidly connecting said bioreactor with a culture medium supply and a gas or gaseous mixture; fluidly connecting said bioreactor with a downstream unit; and growing cells to a density at least 50 million cells per ml. The bioreactor total volume is at least 10 liters. The invention also provides a system suitable for implementation of the method. The system is a small-scale cupboard-sized system and can be placed in a portable clean room.

Thermostatic medium hot water system for lysozyme fermentation liquid dispensing cylinder

本实用新型公开了一种用于溶菌酶发酵液配料缸的恒温介质热水系统，其包括热水缸、热水泵、管式热交换器、蒸汽薄膜调节阀、温度传感器、浮球式疏水阀，热水缸的出口通过管道与热水泵连接，热水泵的出口通过管道接入管式热交换器的热水通道，管式热交换器与蒸汽薄膜调节阀连接，管式热交换器的热水通道出口与温度传感器连接，该温度传感器控制蒸汽进入端的蒸汽薄膜调节阀的开启程度，管式热交换器的蒸汽出口端与浮球式疏水阀连接。本实用新型用于溶菌酶发酵液配料缸的恒温介质热水系统能保持介质热水恒温。

Biological enzyme fermenting installation

本发明公开了一种生物酶发酵装置，包括机壳，机壳内设有发酵腔，发酵腔下端壁内设有旋转腔，旋转腔后端壁内设有带轮腔，带轮腔上端壁内设有与旋转腔连通的啮合腔，带轮腔后端壁内设有动力腔，发酵腔左右端壁内对称设有两个储液腔，从动腔后端壁内设有传动腔，机壳下端面固定连接有底座，机壳内设有搅拌机构、换液机构、传动机构，通过搅拌机构中设有的在旋转搅拌同时可上下往复运动的搅拌块搅拌块，使搅拌更加均匀，保证了搅拌效果，通过设置的换液机构实现自动换液，保证了换液过程中设备的密封性，避免了因人工上料、出料可能导致的设备的污染。

Methods and systems for optimizing perfused cell culture systems

A kind of production method and device of high-efficiency feed enzyme

本发明公开了一种高效饲料酶的生产方法及装置，主要包括以下步骤：S1：发酵浓缩:选取多种菌种，分别经过发酵制备成各酶的初始发酵液；将初始发酵液经过离心过滤浓缩后得到各酶的浓缩酶液；S2：修饰处理:将各酶的浓缩酶液进行修饰处理；S3:制备原料酶液:将修饰的浓缩酶液进行沉降处理得到各原料酶液；S4：混合酶液:选取所制备的各原料酶液，加入保护剂混合后，依次添加中草药提取物、甘露醇和氯化锌，混合均匀后得到混合酶液；S5：高效饲料酶:将混合酶液浓缩干燥后得到固体高效饲料酶。总之，本发明生产的饲料酶具有酶活性高，稳定性强，不易失活，效用高的优点。

System, apparatus and method for biomolecules production

The current invention concerns an automated method for the production of cells and/or biomolecules such as protein or peptides comprising the steps of culturing cells in at least one high cell density bioreactor, thereby fluidly connecting said bioreactor with a culture medium supply and a gas or gaseous mixture; fluidly connecting said bioreactor with a downstream unit; and growing cells to a density at least 50 million cells per ml. The bioreactor total volume is at least 10 liters. The invention also provides a system suitable for implementation of the method. The system is a small-scale cupboard-sized system and can be placed in a portable clean room.

Fermentation process

The present invention relates to improved processes for culturing bacteria, in particular to processes for perfusion suspension culturing of bacteria in a fermenter, wherein the culture medium including the bacteria is circulated over a separation system in alternating tangential flow, wherein the separation system removes a filtrate containing inhibitory metabolites from the culture medium.

Technological device for preparing L-carnitine enzymes

本发明涉及一种制备左旋肉碱酶的工艺装置，包括环形外壳（1）、刀片、所述刀片分两部分，其中一半为光滑环形刀片（2），另一半为齿状刀片（3），该光滑环形刀片（2）与所述齿状刀片（3）之间活动连接，且嵌入式放置在环形外壳（1）内；所述环形外壳（1）分两部分半圆环，其中一半圆环为固定端，另一半圆环为可拆端，使用所述刀片时，将所述的可拆端的半圆环与所述固定端分离拆开；不使用所述刀片时，该可拆端的半圆环与所述固定端相适配安装，形成一个封闭完整的圆环。本发明适用于家庭、商务、工作等多种场合。

A kind of biological dispensing equipment

本发明涉及一种生物配料设备，它包括搅拌缸体、搅拌转轴、搅拌杆、喷水管、吹风管和过滤筛体，搅拌缸体的顶部设置有一分解酶进口，在搅拌缸体的顶部均匀设置有一吹风管，吹风管与搅拌缸体内部连通，在搅拌缸体顶部设置有发酵气混合室，所述发酵气混合室与搅拌缸体的内部连通，过滤筛体设置在搅拌缸体的下部，在搅拌缸体的底部设置有搅拌转轴，搅拌转轴向左右方向设置有搅拌杆，在搅拌缸体的两侧开设有喷水口，所述喷水口与喷水管连通。本发明结构简单，操作简单，配制生物料均匀性好。

Preparation device and method for biological imprinting enzyme

本发明公开了一种用于生物印迹酶的制备装置及方法，包括主反应皿、内胆、顶盖以及检测组件，所述主反应皿为顶端开口的空腔容器，所述主反应皿上开设有放置槽，所述内胆放置于主反应皿的放置槽内，所述顶盖铰接于主反应皿顶端开口上，本发明的有益效果是，该用于生物印迹酶的制备装置配合制备方法，利用专用的设备，将温控、检测等功能集成为一体，从而有效节约生物印迹酶制备的设备占据空间，同时生产方法的实践过程完全自动化控制，能够有效节约人力成本以及时间成本，制备速率更快，能够有效提高制备的效率，既能满足实验室内的少量制备，也能满足大批量的加工制备。

Methods and systems for optimizing perfusion cell culture system

PROCESS AND PLANT FOR THE CONTINUOUS PRODUCTION OF FRUCTOSE-1,6-DIPHOSPHATE BY USING IMMOBILIZED BEER YEAST.

Breathing window type solid state fermentation system

呼吸窗式固态发酵系统，涉及一种固态发酵设备。根据热力学原理设计的，包括呼吸窗式固态发酵罐和固态发酵控制间，固态发酵控制间设有空气温度和湿度调控装置，无菌空气和冷却循环水系统，发酵罐处在固态发酵控制间内，可通过调控固态发酵控制间的温度和湿度有效控制固态发酵罐内的温湿度。发酵罐为薄金属板长方体罐体，罐体的前门门扇和后壁上安有呼吸窗，并设有一个依次由磁力耦合驱动的旋涡风泵、循环通风管、循环通风管水冷却器、气体分布室、平行风导流管、引流管和气体汇集室组成的强制通风供氧导热循环系统，罐内有可移动的可放多层固态发酵浅盘的浅盘架和浅盘，可进行微生物的纯培养，无三废，适用于生物酶制剂等的产业化生产。

Apparatus for converting to adenosine triphosphate

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Fermentation process

The present invention relates to improved processes for culturing bacteria, in particular to processes for perfusion suspension culturing of bacteria in a fermenter, wherein the culture medium including the bacteria is circulated over a separation system in alternating tangential flow, wherein the separation system removes a filtrate containing inhibitory metabolites from the culture medium.

Method for producing cellulase, and apparatus for said method

Provided is a method of producing cellulase comprising the following steps of (1) to (3) wherein the obtained cellulase can be effectively used in detergent application and applications such as hydrolysis of starch and hydrolysis of cellulose; (1) the step of subjecting an aqueous solution of cellulase derived from filamentaous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and to concurrently obtain a concentrated enzyme liquid as a retentate; (2) the step of further subjecting the filtrate obtained in the step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) the step of mixing the concentrated enzyme liquid obtained in the steps of (1) and (2) to obtain cellulase derived from filamentaous fungi.

Process for the production of lignolytic enzymes using Phanerochaete Chrysosporium

Method for producing cellulase, and apparatus for said method

Decellularization and recellularization of organs and tissues

Apparatus for preparing immobilized-enzyme beads and method for preparing immobilized-enzyme beads using same

The present invention relates to an apparatus for preparing enzyme-immobilized beads used for preparation of tagatose, and a method for preparing enzyme-immobilized beads using the same. More specifically, the present invention relates to an apparatus for preparing enzyme-immobilized beads, comprising a nozzle with an inside diameter of 0.1-1 mm having a cylindrical lower end and comprising a cut-type liquid outlet (cut perpendicularly to the vertical axis of the nozzle) formed at the lower end, and a method for preparing enzyme-immobilized beads using the same.

Sterility ultrafiltration concentrated type post extraction product line for fermentation liquor of enzymic preparations

本实用新型公开了一种无菌超滤浓缩型酶制剂发酵液后提取生产线，解决了现有技术工艺简单粗糙、易受外来杂菌污染等问题，其特征在于把发酵液储罐与板框压滤机、压滤清液储罐、微滤膜过滤机、微滤超滤后浓缩液储罐、超滤膜过滤机通过阀门、泵相连通，在压滤清液储罐、微滤膜过滤机之间设置消毒液循环支路系统。本实用新型综合采用了板框压滤、管式膜微滤、卷式膜超滤的密封式过滤设备，充分利用各设备的优势，在除菌除渣、纯化有效成分、减少劳动强度、节能减排等方面取得了显著的效果，利用液体消毒灭菌剂对管路、过滤膜、储罐、动力设施等进行彻底消毒，避免杂菌的污染。

Microfluidic bioreactor with modular design for synthesizing cell metabolites, method for using same, and use thereof

Perfusion bioreactor and related methods of use

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

Finished product residual liquid recovery type enzyme preparation fermentation liquid post-extracting production line

本实用新型公开了一种酶制剂发酵液后提取过滤设备残留液回收系统，解决了现有技术工艺简单粗糙、浪费发酵液的问题，其特征在于把发酵液储罐与板框压滤机、压滤清液储罐、微滤膜过滤机、微滤超滤后浓缩液储罐、超滤膜过滤机通过阀门、泵相连通，在微滤膜过滤机的出口设置无菌空气支路。本实用新型综合采用了板框压滤、管式膜微滤、卷式膜超滤的密封式过滤设备和无菌空气清理残留液设施，充分利用各设备的优势，在除菌除渣、纯化有效成分、回收成品残留液、减少劳动强度、节能减排等方面取得了显著的效果。

Directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of defined enzyme and metabolite mixture and bioreactor

The present invention provides a method for directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of deflned enzyme and metabolite mixtures, enzyme and metabolite materials obtained by such method and suitable devices therefore.

System, apparatus and method for biomolecules production

An automated method for the production of cells and/or biomolecules such as protein or peptides includes culturing cells in at least one high cell density bioreactor, thereby fluidly connecting said bioreactor with a culture medium supply and a gas or gaseous mixture; fluidly connecting said bioreactor with a downstream unit; and growing cells to a density at least 50 million cells per ml. The total volume of the bioreactor is at least 10 liters. A system suitable for implementation of the automated method above is a small-scale and cupboard-sized system, which can be placed in a portable clean room.

Production device of magnetically-recyclable biological enzymes suitable for industrialized application

本发明公开了一种适用于工业化应用的可磁性回收生物酶的生产装置，包括反应釜，所述釜体上部设有原料入口、回用生物酶溶液入口，下部有混合液的出口，出口与分离釜相连，分离釜的下部有管道通过塑料泵与反应釜相连，同时另一出口通过塑料泵与清水池相连。分离釜和清水池的下部都有一个强力电磁铁，通电后产生强磁对得到的可磁性回收生物酶催化剂进行磁力回收，达到快速分离的目的，使催化剂和液体分离加快处理。清水池的上部通过管道与塑料泵的和分离釜相连。本发明设计的装置即可用于可磁力回收催化剂本身的工业化生产，也可用于工业中使用可磁力回收、可重复使用的催化剂的其他工业生产装置。

Semisterile culturing of microbial mixed populations for the preparation of enzyme and metabolite mixtures

The present invention provides a method for directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of deflned enzyme and metabolite mixtures, enzyme and metabolite materials obtained by such method and suitable devices therefore.

Fermentation with cyclic pulse-pause feeding

本发明涉及生产有价值的化合物的方法，包括如下步骤：a) 在发酵培养基中发酵丝状细菌或真菌(例如链霉属菌株或曲霉属菌株)，在发酵期间采用循环脉冲定量给料/暂停的方式在所述发酵培养基中加入糖类，其中所述脉冲定量给料的时间短于暂停时间；和b)从发酵培养液中回收有价值的化合物。

Apparatus for preparing enzyme-immobilized beads and method for preparing enzyme-immobilized beads using same

The present invention is intended to provide an apparatus for preparing enzyme-immobilized beads optimized for stable mass production of tagatose, and a method for effectively immobilizing an isomerase or microbial cells capable of producing the isomerase to a carrier. In the present invention, an apparatus for preparing enzyme-immobilized beads including : a) a first tank into which a mixed liquid containing an enzyme-containing material and an excipient is injected; b) a nozzle unit disposed at a lower end of the first tank, and comprising a nozzle which has an inner diameter of 0.1 mm to 1 mm and a cylindrical lower end and includes a liquid outlet cut perpendicularly to a vertical axis of the nozzle at the lower end thereof; and c) a second tank disposed below the nozzle unit and containing a calcium chloride solution, and a method for preparing enzyme immobilized beads employing the apparatus are provided.

A kind of raw material collection device of novel urokinase extraction

本发明公开了一种新型尿激酶提取用的原料收集装置，包括固定安装在墙壁上的小便器以及固定安装在地面中的固定管，所述小便器下端安装有与所述小便器连通的出水管，所述固定管内壁上左右对称设置有左凸出块和右凸出块，所述固定管中位于所述右凸出块下端设置有通槽，所述固定管下端设置有安装腔，所述固定管下端伸入到所述安装腔中，所述安装腔中设置有与所述固定管下端固定连接且与排污系统连通的排污管，所述安装腔右端壁上端设置有左右延伸的滑动腔，所述滑动腔中可左右滑动地安装有磁性体，所述滑动腔右端壁中固定设置有铁芯，所述铁芯外周上固定安装有线圈，所述通槽中可左右滑动地安装有滑动管。

Preparation device and preparation method of solid-state fermentation enzyme preparation

本发明公开了一种固态发酵酶制剂的制备装置，涉及固态发酵酶制剂的制备技术领域，包括发酵罐，发酵罐的顶部设置有进料口，且进料口处安装有盖体，所述发酵罐的底部设置有出液管，且出液管上设置有阀门，还包括设于罐体内部的隔离机构和搅拌机构，该隔离机构包括固定在罐体的内侧壁上的环状隔板，以及连接在环状隔板内侧并向下延伸形成的筒状隔板；所述筒状隔板的内侧形成有搅拌腔，所述筒状隔板与罐体的内侧壁之间形成有环状腔。本发明使得发酵液不断通过环状腔中后从排液孔排出，在整个过程中，发酵液形成一个流动状态，增加了发酵液与加热管的接触面积，提升搅拌效果，使得发酵液整体均匀的被加热，避免发酵液各部分受热不均匀。

Apparatus for preparing enzyme-immobilized beads and method for preparing enzyme-immobilized beads using same

Directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of defined enzyme and metabolite mixture and bioreactor

Solid cultivation method of nattokinase

本发明公开了一种纳豆激酶的固体培育方法，通过将纳豆枯草杆菌的液体菌种接种到培养基上，纳豆枯草杆菌的接种量为8‑12％；培养基包括如下组分：大豆蛋白胨1％，牛肉膏0.5％，碳酸氢钠0.8％，氯化钙0.03‑0.04％，硫酸镁0.02‑0.04％。本发明的培养基中加入的碳酸氢钠在进行pH调节时产生的气体以及通过酵母菌发酵产生的二氧化碳能使得培养基更为疏松，提高了培养基的比表面积，利于纳豆枯草杆菌培育，提高纳豆激酶的产量及活性；培育时的培育装置采用的隔挡能将培育箱划分为若干个不同的培育区域，满足实际中的不同使用需求；将托盘与转动装置相连，使得菌种培育完成后，通过转动装置的运行能更快捷地将培养基倾倒至培育箱的底部，具有方便快捷，操作效率高的特点。

Enzyme production using one or more co-product compositions from a bioprocessing facility

Enzyme production in a bioprocessing facility that utilizes the enzyme that is produced. Enzyme is produced by one or more microorganisms grown on a co-product (e.g., one or more stillage compositions) of the bioprocessing facility.

Anaerobic fermentation tank for enzyme preparation production and use method

本发明公开了一种酶制剂生产用厌氧发酵罐及使用方法，包括罐体和出料仓，出料仓外表面连接有三根支撑柱；罐体顶部连接有顶盖，顶盖下方的罐体上表面设置有电机，电机输出端连接有转轴，转轴延伸至罐体内部；电机一侧的罐体上表面设置有进料管，电机另一侧的罐体上表面设置有冲洗装置；冲洗装置包括水箱和水泵，水泵出水端连接有冲洗管，冲洗管位于罐体内部；转轴外表面等间距套设有多组搅拌叶，相邻的两组搅拌叶之间的转轴外表面套设套管，套管侧表面连接有连杆，连杆一端连接有刮板与罐体内壁接触配合。该装置解决了现有技术对罐体内部清洗效率低、用水量大、清洗效果差的问题，具有操作简单，清洗效果好、无需大量用水，且清洗效果佳的特点。

A kind of hydrolysis reactor and its application method handling solid waste

本发明具体涉及一种水解反应器，通过对固体废弃物进行超高温处理产生特异性酶制剂，加速细胞的破碎溶出，加速厌氧消化。产酶区的待处置固体废弃物可由于高温作用产生酶，酶是固体废弃物内微生物的产物，不需要额外投加；产酶区的固体废弃物持续性产生酶制剂，不断供给厌氧消化使用；不同固体废弃物因其构成的差异产生的酶会有所不同，但某类固体废弃物产生的酶对该类物质更具有特异性和针对性，更有助于此类物质的厌氧消化。产酶、储酶、水解一体化，实现了酶制剂的持续产生和连续投加；利用该反应器的污泥消化工艺，可在3~15d内将污泥挥发性固体减量40%~60%；利用该反应器的餐厨垃圾消化工艺，可在3~15d内将餐厨垃圾挥发性固体减量40%~80%。反应器适应性强，污泥减量化效果好，且能够产生更多的甲烷，加速污泥或餐厨垃圾减量化过程，节省固体废弃物处置成本。

DEVICE FOR COMPOSTING AND THE SIMULTANEOUS USE OF BIOGAS

一种生物酶制备用微生物发酵罐及发酵方法

本发明公开了一种生物酶制备用微生物发酵罐及发酵方法，涉及微生物发酵罐技术领域，包括发酵罐体，发酵罐体设有发酵微生物的发酵腔室，发酵腔室的内部顶端通过内螺纹转动插接有罐盖，罐盖能够封闭发酵罐体的发酵腔室，安装孔的圆弧形结构转动卡接有球型调节块，插接孔内滑动插接有搅拌杆，搅拌杆的底端贯穿插接孔后延伸至发酵腔室内部，搅拌杆位于发酵腔室内部的一端开设有T型槽，T型槽内滑动设有矩形滑块，矩形滑块底端固定连接有延伸出T型槽的连接杆，连接杆伸出T型槽的一端径向侧壁设有压板。本发明无需要频繁打开罐盖进行搅拌操作，避免发酵腔室内灌入空导致微生物氧化或者灭活，影响微生活的发酵成品率，提高发酵成本。

一种工业酶样品提取设备

本发明公开了一种工业酶样品提取设备，涉及工业酶制备技术领域，其技术方案要点是取样元件包括提取外筒，滑动安装在工业酶设备的内部，所述提取外筒上开设有一通道，在该通道处设置有一个封装板，该封装板用于将所述通道开启或封闭；提取内筒，转动安装在提取外筒的内部，所述提取内筒的外表面与提取外筒的内表面紧密贴合，所述提取内筒的圆周方向上等间隔的设置有多个分隔板，多个分隔板将提取内筒的内部分隔成多个提取腔，所述提取内筒的外表面上开设有多个第一通孔，每个提取腔处均具有第一通孔；间歇性单向转动机构，设置在提取外筒的一端处，且与提取内筒固定连接，效果是可以一次性提取多个不同深度的工业酶样品，提升了提取效率。

一种制备酶工艺的陶瓷高分子膜设备

本发明公开了一种制备酶工艺的陶瓷高分子膜设备，包括底座，底座下表面四周分别通过螺栓与万向轮和支撑座固定连接，万向轮一侧设置有紧急制动装置，机架上端内侧分别通过螺栓与酶原液储罐下端外侧固定连接，机架一侧分别通过螺栓与控制箱固定连接，机架一侧分别设置有第一流量计、第二流量计和第三流量计，机架另一侧分别设置有第一陶瓷高分子膜和第二陶瓷高分子膜。本制备酶工艺的陶瓷高分子膜设备，整体生产过程自动化高，运行可靠，大大降低了劳动强度，形成阶梯式分离，工序简单，流程短，大大缩短了生产周期，膜工艺过程不发生相变化，无需加热，大大节省能耗，过滤精度高，杂质含量少，有效的提高了产品质量。

一种采用米曲霉生产酸性乳糖酶的方法及制备装置

本发明涉及乳糖酶生产技术领域，具体公开了一种采用米曲霉生产酸性乳糖酶的方法及制备装置，其包括如下步骤：取适量米曲霉接种到斜面培养基上活化三天，备用；取步骤1中活化后的菌株斜面用生理盐水制成孢子液；向第一培养罐、第二培养罐和第三培养罐中分别放入培养液，然后将孢子液依次通入对应培养罐中进行培养，然后再液体泵入低压浓缩罐中进行浓缩；浓缩完成后通过浆料泵将浓缩液泵入喷雾干燥塔中进行喷雾干燥即得酸性乳糖酶；本发明公开的采用米曲霉生产酸性乳糖酶的方法，其配合整套生产设备能够实现酸性乳糖酶的全自动化生产，从培养到浓缩，再到对浓缩液的喷雾干燥，其能够制备生产出乳糖酶粉料，其成功实现了乳糖酶的工业化生产。

一种固定化里氏木霉高产纤维素酶的方法

本发明公开了一种固定化里氏木霉高产纤维素酶的方法。通过采用一定密度、孔径和外观直径的聚氨酯泡沫作为里氏木霉吸附的固定化载体，利于系统传质作用，可减少工业放大生产过程中的能耗。将利用改性剂聚醚酰亚胺改性后的固定化载体置于生物反应器的固定化载体区域使其自由悬浮更好的吸附里氏木霉，再通过调节发酵培养中的碳氮源和发酵方式，调控菌体的生长，减少菌体量过多对传质带来的影响，同时也促进了酶活的提升，有利于实现长期稳定的固定化连续发酵纤维素酶。

恒温水浴式复合酶三角瓶摇瓶装置

一种恒温水浴式复合酶三角瓶摇瓶装置，包括固定装置、涡流发生装置、竖向支撑装置、摇动装置、喷淋装置、加热装置，固定装置内部设置有温水，固定装置内部还设置有涡流发生装置，三角瓶同时受温水浮力及涡流发生装置产生涡流的作用力，使固定装置内部的三角瓶进行左右摇摆、上下振动，进而使三角瓶摇瓶充分，促使三角瓶内的物料与菌种松散透气，防止菌种结块。同时涡流发生装置功率可调节，有利于根据扩陪工艺优选出最佳的工作功率，以使三角瓶摇瓶效果达到最佳；还设置有喷淋装置，以降低三角瓶内部菌种发酵的温度；还设置有加热装置，以使箱体内部的三角瓶始终处于25℃‑35℃的恒温水浴环境中，有利于三角瓶内部菌丝生长。

一种肽酶原核发酵装置及肽酶原核的加工方法

本发明公开了一种肽酶原核发酵装置，包括发酵罐和罐体，发酵罐通过柔性件连接所述罐体；罐体内部设置内分离箱，内分离箱内侧底部安装有主体转动座，主体转动座上方安装有主体转动座上方安装有分离套筒底座，分离套筒底座顶部安装有顶部套环座，罐体顶部设置有顶盖，顶盖上方的中间位置设置有密封顶盖，顶部套环座安装在顶盖下方，本发明涉及提取物分离技术领域。该肽酶原核发酵装置，解决了固液提取物在发酵完成后提取时效率低下，并且在提取的时候不能快速的分离固体的残渣，还容易导致提取液回流，装置提取分离液溶液相互污染的问题，两个不同方式获取的提取液，采用了单独的分离外管排出，使得装置在排出的时候不会相互干扰。

恒温水浴式复合酶三角瓶摇瓶装置

一种恒温水浴式复合酶三角瓶摇瓶装置，包括固定装置、涡流发生装置、竖向支撑装置、摇动装置、喷淋装置、加热装置，固定装置内部设置有温水，固定装置内部还设置有涡流发生装置，三角瓶同时受温水浮力及涡流发生装置产生涡流的作用力，使固定装置内部的三角瓶进行左右摇摆、上下振动，进而使三角瓶摇瓶充分，促使三角瓶内的物料与菌种松散透气，防止菌种结块。同时涡流发生装置功率可调节，有利于根据扩陪工艺优选出最佳的工作功率，以使三角瓶摇瓶效果达到最佳；还设置有喷淋装置，以降低三角瓶内部菌种发酵的温度；还设置有加热装置，以使箱体内部的三角瓶始终处于25℃‑35℃的恒温水浴环境中，有利于三角瓶内部菌丝生长。

一种纳豆激酶的固体培育方法

本发明公开了一种纳豆激酶的固体培育方法，通过将纳豆枯草杆菌的液体菌种接种到培养基上，纳豆枯草杆菌的接种量为8‑12％；培养基包括如下组分：大豆蛋白胨1％，牛肉膏0.5％，碳酸氢钠0.8％，氯化钙0.03‑0.04％，硫酸镁0.02‑0.04％。本发明的培养基中加入的碳酸氢钠在进行pH调节时产生的气体以及通过酵母菌发酵产生的二氧化碳能使得培养基更为疏松，提高了培养基的比表面积，利于纳豆枯草杆菌培育，提高纳豆激酶的产量及活性；培育时的培育装置采用的隔挡能将培育箱划分为若干个不同的培育区域，满足实际中的不同使用需求；将托盘与转动装置相连，使得菌种培育完成后，通过转动装置的运行能更快捷地将培养基倾倒至培育箱的底部，具有方便快捷，操作效率高的特点。

一种过氧化物酶制备装置及工艺

本发明提供一种过氧化物酶制备装置及工艺，包括罐体、粉碎桶、搅拌桶，所述罐体顶部罐身一侧贯穿设有加注口，且罐体底部罐身一侧贯穿设有排污口，所述罐体中心设有用于控制内部设备的控制面板，且控制面板一侧的罐体上设有观察窗，所述罐体底部贯穿设有驱动电机，且罐体内部上下两端固定连接有固定转轴，所述罐体内部上端的固定转轴上转动连接有粉碎桶，同时罐体内部下端的固定转轴上转动连接有搅拌桶，所述驱动电机输出端啮合连接有驱动杆，本发明提供一种过氧化物酶制备装置及工艺为解决原料无法充分利用造成原材料浪费增加原料成本的问题，及加工工序无法顺利衔接导致需要增加操作人员造成人员成本增加的问题。

一种胃蛋白酶提取设备及提取方法

本发明涉及胃蛋白酶加工技术领域，具体的说是一种胃蛋白酶提取设备及提取方法,一种胃蛋白酶提取设备，包括电机、第一伸缩杆、捣头、外壳和内壳，所述电机固定在外壳顶部，所述电机的输出轴伸入外壳内部并固连有第一伸缩杆，且所述第一伸缩杆底端位置固连有捣头；通过设置过滤网、封闭环、第二伸缩杆和顶块，顶块为铁质且过滤网下表面的中间部位均匀设有磁铁，当第二伸缩杆通过封闭环带动着顶块向下移动时，过滤网下表面将逐渐被拉扯成锥形，且当顶块对过滤网的拉力大于磁铁的吸引力时，顶块将与过滤网断开接触，且具有弹性的过滤网将会迅速向上弹起，从而自动清理过滤网表面上的黏膜液，进一步提高了后续过滤网过滤提取液的效果。

一种采用米曲霉生产酸性乳糖酶的方法及制备装置

本发明涉及乳糖酶生产技术领域，具体公开了一种采用米曲霉生产酸性乳糖酶的方法及制备装置，其包括如下步骤：取适量米曲霉接种到斜面培养基上活化三天，备用；取步骤1中活化后的菌株斜面用生理盐水制成孢子液；向第一培养罐、第二培养罐和第三培养罐中分别放入培养液，然后将孢子液依次通入对应培养罐中进行培养，然后再液体泵入低压浓缩罐中进行浓缩；浓缩完成后通过浆料泵将浓缩液泵入喷雾干燥塔中进行喷雾干燥即得酸性乳糖酶；本发明公开的采用米曲霉生产酸性乳糖酶的方法，其配合整套生产设备能够实现酸性乳糖酶的全自动化生产，从培养到浓缩，再到对浓缩液的喷雾干燥，其能够制备生产出乳糖酶粉料，其成功实现了乳糖酶的工业化生产。

一种生物酶培养用反应设备

本发明涉及生物酶制备技术领域，具体提出了一种生物酶培养用反应设备；包括发酵反应釜以及搅拌总成；所述搅拌总成包括装配在所述发酵反应釜中的外置搅拌机构以及装配在所述外置搅拌机构中的内置匀料机构；与现有的采用简单结构的搅拌桨式的同类设备相比，本发明中提供的设备中包含外置搅拌机构以及内置匀料机构两套混料系统的搅拌总成，对于发酵物料具有混合搅拌、搅动分散以及喷射冲击的综合混料功能，提高了多相物料之间的综合混合接触效果，进而提高了发酵物料的利用率、生物酶的生成效率以及发酵生成量。

一种生物酶培养用反应设备

本发明涉及生物酶制备技术领域，具体提出了一种生物酶培养用反应设备；包括发酵反应釜以及搅拌总成；所述搅拌总成包括装配在所述发酵反应釜中的外置搅拌机构以及装配在所述外置搅拌机构中的内置匀料机构；与现有的采用简单结构的搅拌桨式的同类设备相比，本发明中提供的设备中包含外置搅拌机构以及内置匀料机构两套混料系统的搅拌总成，对于发酵物料具有混合搅拌、搅动分散以及喷射冲击的综合混料功能，提高了多相物料之间的综合混合接触效果，进而提高了发酵物料的利用率、生物酶的生成效率以及发酵生成量。

Methods and systems for propagating microorganisms for mitigating mycotoxin contamination, and related systems and methods

Systems and methods for propagating at least one microorganism expressing one or more enzymes to mitigate mycotoxin contamination at a bioprocessing facility. The systems are adapted to supply a composition derived from the culture tanks and comprising the culture broth, the microorganisms, a lysate of the microorganisms, the isolated enzyme and/or a combination of these components at different points of a bioprocessing facility.

固定化纤维素酶、固定化纤维素酶的制备方法及制备装置

固定化纤维素酶、固定化纤维素酶的制备方法及制备装置，固定化纤维素酶的制备方法包括以下步骤：1)、将海藻酸钠溶液、纳米二氧化硅、纤维素酶以及戊二醛混合均匀后滴入氯化钙溶液中，得到固定化酶小球；2)、将步骤1)中得到的固定化酶小球浸泡在聚乙烯亚胺溶液中，并经紫外线照射；3)、将经步骤2)处理过的固定化酶小球在氯化钙溶液中浸泡后取出，即得到固定化纤维素酶。本发明可使纤维素酶可多次重复利用，提高其利用率。

Integrated continuous bioprocess production platform

The present invention relates to the field of biopharmaceutical manufacturing, in particular that of protein production. More specifically, the present invention relates to a modular cell-based production system designed for the production of monoclonal antibodies and other therapeutic proteins. The invention further relates to a perfusion bioreactor, in particular a fully continuous and integrated bioprocess designed for the production of monoclonal antibodies and other therapeutic proteins.

一种工业酶生产的原料发酵设备

本发明公开了一种工业酶生产的原料发酵设备，其结构包括发酵罐、驱动电机、支撑台、防护栏，发酵罐顶端与驱动电机整体螺栓固定，发酵罐四周与防护栏内侧间隙配合，防护栏底部与支撑台上表面焊接连接，支撑台上表面与发酵罐底部嵌固连接，发酵罐包括搅拌器、导流槽、活动腔、添料口，搅拌器整体与活动腔内部活动配合，活动腔底部与导流槽上表面嵌套连接，本发明通过驱动杆形成的离心力惯性将其刮条体向外甩出，以至由卡扣与防脱块防止与其脱落，使得甩出后的刮条体和活动腔内壁形成贴合刮动，通过硅胶块的刮动与导流槽的导流，使其附着在腔内壁的酶块形成脱落，从而有效的降低因为酶块附着在腔内壁而造成的排放原料配比不均的问题。

一种光触型塑料分解酶及其制备装置

本发明涉及塑料分解酶技术领域，具体涉及一种光触型塑料分解酶及其制备装置；通过黄粉虫干粉与磷酸钠缓冲液共同研磨，然后进行分离，收集上清液，然后在上清液中加入硫酸铵溶液进行沉淀，并冷冻离心，收集第二上清液，然后在第二上清液中加入的硫酸铵溶液进行沉淀，并进行离心，然后将沉淀物溶解于磷酸钠缓冲液中，并静置过夜，然后离心，得到第三上清液，然后对第三上清液在磷酸钠缓冲液中透析过夜，并进行纯化和浓缩，再利用氯化钠溶液进行线性洗脱，获得黄粉虫提取物，最后将黄粉虫提取物与乙酸乙酯与水的乳化溶液进行混合，制得塑料分解酶，能有效分解聚苯乙烯泡沫塑料，并分解效率较理想。

一种制备酶工艺的陶瓷高分子膜设备

本发明公开了一种制备酶工艺的陶瓷高分子膜设备，包括底座，底座下表面四周分别通过螺栓与万向轮和支撑座固定连接，万向轮一侧设置有紧急制动装置，机架上端内侧分别通过螺栓与酶原液储罐下端外侧固定连接，机架一侧分别通过螺栓与控制箱固定连接，机架一侧分别设置有第一流量计、第二流量计和第三流量计，机架另一侧分别设置有第一陶瓷高分子膜和第二陶瓷高分子膜。本制备酶工艺的陶瓷高分子膜设备，整体生产过程自动化高，运行可靠，大大降低了劳动强度，形成阶梯式分离，工序简单，流程短，大大缩短了生产周期，膜工艺过程不发生相变化，无需加热，大大节省能耗，过滤精度高，杂质含量少，有效的提高了产品质量。

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筛选装置及含其的混料机

本实用新型涉及混料技术领域，首先公开了筛选装置，其内部设置有筛选箱，所述筛选箱的内部固定设置有筛选网，所述筛选网呈中间高两侧低的倒V状，所述筛选箱外壁的两侧以及筛选装置内壁的两侧均固定设置有支撑板，所述支撑板的数量为两个，且两个支撑板之间活动设置有第一弹簧，所述第一弹簧的两端均固定设置有固定板，所述固定板的两侧均开设有卡槽；其次，本实用新型公开了含该筛选装置的混料机，该混料机还包含在所述筛选装置的底部固定设置的搅拌装置。通过设置筛选网，可以对体积较大的原料进行筛选处理，体积较大的原料会停滞在筛选网的顶部，体积均匀的原料会落入到搅拌箱内，避免了体积较大的原料混入到搅拌箱内而影响混料的质量。

一种酶制剂生产装置

本发明涉及酶制剂生产技术领域，尤其涉及一种酶制剂生产装置，包括过滤罐体，过滤罐体的内部固定焊接有水平设置的隔板，隔板将过滤隔板的内部分割成空气净化腔和压滤腔，过滤罐体的顶端固定安装有空气净化器，空气净化器的出气口固定连接有进气管，进气管与空气净化腔的内部连通，过滤罐体的侧壁上固定安装有气泵，气泵的进气口与空气净化腔的内部通过管道连通，气泵的进气口与压滤腔的内部通过管道连通，空气净化腔的内壁上固定安装有紫外线灯，压滤腔所在的过滤罐体的侧壁上固定设置有进料管，进料管与压滤腔的内部连通。本发明解决了现有技术中存在压滤时没有对空气进行过滤处理的缺点，有益效果明显，适合推广。

一种制药工业用酶制备设备

本发明公开了一种制药工业用酶制备设备，涉及制药技术领域，包括输送机构、清洗机构、回收机构、输送斜板、破碎发酵机构和离心提纯机构，所述清洗机构设置在所述输送机构的旁侧，所述清洗机构的输出端延伸至所述输送机构上方，所述回收机构设置在所述输送机构的下方，所述输送斜板固定设置在输送机构的输送端上，所述破碎发酵机构设置在所述输送机构靠近所述输送斜板的一侧，并且所述破碎发酵机构位于所述输送斜板的下方，所述离心提纯机构设置在所述破碎发酵机构的旁侧，所述离心提纯机构与所述破碎发酵机构之间相连通。本发明通过各个组件之间配合工作完成制药用酶一体化的加工生产作业，使用性较高。

Dezellularisierung und rezellularisierung von organen und geweben

脂肪組織再生基材

本発明は、取り扱い性が高く、大体積の脂肪組織を正常な形状で再生することができる脂肪組織再生基材を提供することを目的とする。本発明は、内部空間を有し表面に前記内部空間へ通じる開口部を複数有する生体吸収性材料からなる粒状体と、開口部を有し複数の前記粒状体を包む生体吸収性材料からなる袋状体とから構成される脂肪組織再生基材である。

一种蛋白酶粗提物收集系统及方法

本发明公开一种蛋白酶粗提物收集系统及方法，其系统中：循环箱的底部与缓冲罐的底部连通，其第一内腔与第一泵体的泵入口、第二泵体的泵出口均连通，第一泵体的泵出口、第二泵体的泵入口与第一三通阀的进口端连通，第一三通阀的第一出口端与循环箱的第四内腔的底部连通，第一三通阀的第二出口端与第二三通阀的第二出口端连通，第二三通阀的进口端与循环箱的第三内腔的底部连通，循环箱上设有连通至其内腔中的第一循环支管和第二循环支管；收集箱与循环箱的第二内腔的底部、第二三通阀的第一出口端连通，其底部与排放端连通。本发明能够同步完成两种蛋白酶粗提物的自动化收集，并且能够定制化增减所收集蛋白酶的种类，并针对性控制处理量。

一种聚合多肽酶生产设备及工艺

本发明公开了一种聚合多肽酶生产设备及工艺，涉及到多肽酶生产设备领域，其包括罐体，所述罐体上连接有罐盖，所述罐体上套接有水浴组件，用于对所述罐体进行加热；所述罐体的两侧均安装有气缸。需要说明的是，在本发明中，通过水浴组件和离心固液分离组件的设置，罐体在进行聚合多肽酶的生产时，可以通过水浴槽对罐体进行加热后，反应后，通过分离筒能够实现固液分离，得到所需的液体位聚合多肽酶的初步产物，集成度高，省事省力，使用方便，在分离筒转动进行固液分离时，过滤板能够将分离后的液体进行进一步的过滤，并且在分离筒转动时，压缩板能够将分离筒内的液体能够被充分的挤压出来，提高了生产效率，避免材料的浪费。

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一种用于酶制剂生产的分离装置

本发明公开了一种用于酶制剂生产的分离装置，包括离心箱和浓缩箱，分离装置本体包括离心箱和浓缩箱，离心箱安装在浓缩箱的顶面上，浓缩箱的底面上安装有支腿；离心箱的底部设置有固定板，固定板的顶面上转动安装有离心筒，且固定板的底面上安装有第一电机，第一电机的输出端与离心筒的底面连接，离心筒的侧壁上均匀设置有多组的滤孔，离心筒的顶面外壁上设置有限位环，限位环通过支杆安装在离心箱的内壁上，离心筒的顶面设置有固定环，固定环上卡扣连接有过滤件；本发明具有提高了酶制剂分离的效率、搅拌效率高且提高酶制剂产率的优点。

一种蔗糖转化酶的培养设备及培养方法

本发明涉及一种蔗糖转化酶的培养设备，包括底座，所述底座的内顶壁固定有支撑组件，所述底座的顶部固定有工作仓，工作仓的内部固定有竖向隔板，竖向隔板的正面固定有横向隔板，所述工作仓的内部活动连接有恒温仓，恒温仓的内底壁固定有固定组件，所述工作仓的正面和恒温仓的正面均固定有连接组件，所述工作仓的内部设置有贯穿至竖向隔板和恒温仓后侧壁的恒温机构。该蔗糖转化酶的培养设备及培养方法，打开恒温仓拉动固定组件，将培养皿放置在固定组件上，固定组件对蔗糖转化酶培养皿进行固定，将恒温仓关闭，恒温仓回缩至工作仓内部，连接组件将恒温仓和工作仓进行固定，实现恒温仓固定的目的，避免蔗糖转化酶培养皿因碰撞导致破碎。