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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 13879. Отображено 100.
05-01-2012 дата публикации

Process and Reactor System for Depolymerization of Polymeric Biomass

Номер: US20120003724A1
Принадлежит: Nagarjuna Energy Pvt Ltd

The present invention provides a continuous process for saccharification of cellulose by enzymatic degration without any loss of enzymes and also discloses a bioreactor for performing said process.

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03-05-2012 дата публикации

Microorganism culture sheet and manufacturing method therefor

Номер: US20120107913A1
Принадлежит: DAI NIPPON PRINTING CO LTD

A microorganism culture sheet is provided which can limit the use of a costly cultivation material to a necessary portion, which has simple production at the time of manufacture, and can be simply and stably used by a user. The microorganism culture sheet of the present invention is a microorganism culture sheet comprising a base sheet, a culture layer formed on top of the base sheet, and a cover sheet that covers the culture layer, and the culture layer is pattern-formed with an application liquid comprising a polyvinylpyrrolidone and at least on selected from the group consisting of a gelling agent, a nutrient component, a color indicator, a selective agent and a substrate.

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10-05-2012 дата публикации

Container for testing for micro-organisms

Номер: US20120115216A1
Принадлежит: ZYZEBA TESTING Ltd

A multi-compartment resealable container for testing for the presence of micro-organisms is provided with or adapted to receive a sample in a first substantially rigid transparent compartment, a growth medium in a second compartment, and a sanitizer in a third compartment, the compartments being separated by foil seals, the second and third compartments have an associated plunger which when depressed causes serrated cutters to puncture the foil seal and allow the contents to be added to the liquid containing or comprising the sample.

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24-05-2012 дата публикации

Apparatus For Culturing Anchorage Dependent Cells

Номер: US20120129257A1

The invention relates to an apparatus ( 1 ) for culturing anchorage-dependent cells. The apparatus ( 1 ) comprises a housing ( 2 ) with an inlet ( 4 ) and an outlet ( 5 ), and a plurality of culture plates ( 6 ) removably stacked within the housing ( 2 ). The housing ( 2 ) has a circumferential wall ( 7 ), a base ( 8 ) and a top wall ( 9 ). The base ( 8 ) comprises the inlet ( 4 ) and the top wall ( 9 ) comprises the outlet ( 5 ). The circumferential wall ( 7 ) of the housing ( 2 ) defines a longitudinal axis thereof, as well as an inner cross section perpendicular to the longitudinal axis. Shape and dimensions of the inner cross section are essentially uniform along the longitudinal axis. The culture plates ( 6 ) are arranged at least essentially parallel to each other. Each plate ( 6 ) is mounted and sealed to the circumferential wall ( 7 ) of the housing ( 2 ). Plates ( 6 ) are arranged at a distance from each other. Each culture plate ( 6 ) has a through hole ( 14 ), so that inlet ( 4 ) and outlet ( 5 ) are in fluid communication. Each through hole ( 14 ) is positioned at an outer end of the respective culture plate ( 6 ), proximate the circumferential wall ( 7 ). Through holes ( 14 ) of adjacent culture plates ( 6 ) are distally positioned in the plane of the inner cross section of the housing ( 2 ).

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21-06-2012 дата публикации

Disposable vortex breaker

Номер: US20120155216A1
Принадлежит: EMD Millipore Corp

The present invention consists of a closed presterilized bag having a disposable mixing element within it, a drive mechanism outside of the bag for rotating the mixing element without voiding sterility and a vortex breaker in the torm of one or more plastic sheet materials that are attached to various inner surfaces of the bag and disrupt the formation of vortices within the bag. Preferably the sheet(s) are formed of the same material as the bag and are sealed to the bag surfaces. More preferably, the sheet(s) extend across a diameter of the bag. Most preferably, the sheet(s) are perforated with one or more slits or openings to allow for good flow and mixing without a vortex being formed.

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19-07-2012 дата публикации

Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner

Номер: US20120184009A1
Принадлежит: Individual

A method and device for growing plant, animal or stem cells in a continuous manner.

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09-08-2012 дата публикации

Self-contained breathing closure and container

Номер: US20120199207A1
Принадлежит: Individual

A self-contained breathing closure for flasks and other containers that require gas exchange. An illustrative embodiment of the closure is comprised of a splashguard, an adaptor for attaching the closure to the container, a bellows element and a gas-permeable barrier element. The splashguard is intended to keep liquid contents under vigorous agitation in the container without wetting the gas-permeable barrier. The adaptor couples the closure to the container in a secure fashion. The bellows element allows for repeated changes in the internal volume of the container-closure system. The gas-permeable barrier allows desired gases to enter and leave the container while excluding small particles and/or microorganisms. The technology may be used with existing glass flask technology, or coupled to a plastic flask that may be configured for either single-use, or multiple-use.

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15-11-2012 дата публикации

Configurable port fitment, kit, and related methods

Номер: US20120284991A1
Принадлежит: Matthew Kusz, Vishwas Pethe

A configurable port fitment includes an adapter for forming a locking engagement with a connector associated with a vessel. A kit and related methods may use the configurable port fitment.

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22-11-2012 дата публикации

Novel enhanced surface area conico-cylindrical flask (es-ccf) for biofilm cultivation

Номер: US20120295293A1

A novel enhanced surface area conico-cylindrical flask (ES-CCF) providing increased surface area by virtue of its' inner arrangement and useful in routine small-scale studies of bioactives production by any biofilm-forming marine as well as terrestrial microorganisms. Compared to corresponding Erlenmeyer flask of similar volume the ES-CCF provides more than 80% additional surface for biofilm attachment and growth. The ES-CCF does not require steam sterilization and is durable as the device is constructed of polymethyl methacrylate or any other such hydrophobic material and offers possibility of altering the nature of the growth surface (hydrophilic and hydrophobic). The ES-CCF also provides external aeration like a bioreactor, thus increasing the versatility of applications. Further, the device can be operated as a cylindrical flask, that is without the inner arrangement.

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07-03-2013 дата публикации

Method and system for the production of cells and cell products and applications thereof

Номер: US20130058907A1
Принадлежит: Biovest International Inc

A cell culture system for the production of cells and cell derived products includes a reusable instrumentation base device incorporating hardware to support cell culture growth. A disposable cultureware module including a cell growth chamber is removably attachable to the instrumentation base device. The base device includes microprocessor control and a pump for circulating cell culture medium through the cell growth chamber. The cultureware module is removably attached to the instrumentation base device. Cells are introduced into the cell growth chamber and a source of medium is fluidly attached to the cultureware module. Operating parameters are programmed into the microprocessor control. The pump is operated to circulate the medium through the cell growth chamber to grow cells or cell products therein. The grown cells or cell products are harvested from the cell growth chamber and the cultureware module is then disposed.

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13-06-2013 дата публикации

Use of oxyhydrogen microorganisms for non-photosynthetic carbon capture and conversion of inorganic and/or c1 carbon sources into useful organic compounds

Номер: US20130149755A1
Автор: John S. Reed, Lisa Dyson
Принадлежит: Kiverdi Inc

Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including bio-fuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO 2 in one or more steps of the process.

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25-07-2013 дата публикации

Cell Culture System and Method of Use Thereof

Номер: US20130189671A1
Принадлежит:

The present invention is based in part on the discovery of significant improvements to cell culture systems and methods in terms of utility and range of application. The system allows for culture of cells in one or more defined spaces within a culture chamber defined by one or more spaced opposing surfaces to provide significant advantages over previously described culture methods. The system includes a chamber for receiving a fluid culture medium; a first surface and one or more second surfaces defining one or more spaces in the chamber; and one or more cells constrained within the one or more spaces. The second surface includes at least one opening for passage of a substance into the culture space. 1. A cell culture system , comprising:a) a chamber for receiving a fluid culture medium;b) a first surface and one or more surfaces in opposition thereby defining one or more spaces in the chamber; andc) one or more cells within the one or more spaces;wherein the one or more surfaces comprise one or more openings allowing for passage of a substance into or out of the one or more spaces.2. The cell culture system of claim 1 , wherein the cells are growing on or by other means attached to the internal side of the one or more surfaces.3. The cell culture system of claim 1 , wherein the cells are free floating.4. The cell culture system of claim 1 , wherein the cells are confined by a permeable barrier.5. The cell culture system of claim 1 , wherein one or more of the surfaces are substantially planar.6. The cell culture system of claim 1 , wherein one or more of the surfaces are transparent.7. The cell culture system of claim 1 , wherein one or more of the surfaces are opaque.8. The cell culture system of claim 1 , wherein one or more of the surfaces comprise a cell culture compatible material.9. The cell culture system of claim 1 , wherein each space comprises a major dimension and a minor dimension claim 1 , the minor dimension being defined by the distance between two ...

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01-08-2013 дата публикации

STENCIL PATTERNING METHODS AND APPARATUS FOR GENERATING HIGHLY UNIFORM STEM CELL COLONIES

Номер: US20130196435A1

A method for producing highly uniform cell colonies in a cell culture dish with the use of stencils made from an elastomeric sheet sized to fit within the cell culture dish, having a singular opening or a plurality of openings of a number, pitch and diameter configured to optimally control the geometric growth parameters of a cell colony. The uniform cell colonies are produced by placing the stencil in a cell culture dish and hydropilizing the stencil. The stencil is overlayed with cell culture media and seeded with seed cells that are preferably grown for at least a day before the stencil is removed to produce a pattern of seeded cells with controlled pitch, colony diameter and density within the culture dish that grow to become highly uniform cell colonies. A kit with culture dish, stencil, culture media and growth media is also provided. 1. A method for producing highly uniform cell colonies , comprising:placing a stencil in a cell culture dish patterned with a singular opening or a plurality of openings of a selected size, spacing and density;hydropilizing the stencil;overlaying the stencil with cell culture media;seeding the cell culture media with seed cells;growing the seed cells in the cell culture media; andremoving the stencil to produce a pattern of seeded cells with controlled pitch, colony diameter and density within the culture dish;wherein controlled pitch, colony diameter and density within the culture dish produces highly uniform cell colonies or improves the yield and/or geometric repeatability of stem cell differentiation.2. A method as recited in claim 1 , wherein the stencils are elastomeric stencils cut from a PDMS (polydimethylsiloxane) material.3. A method as recited in claim 1 , wherein said stencils have circular openings with a diameter ranging from approximately 200 μm to approximately 3 mm.4. A method as recited in claim 1 , wherein said stencils have a distance between openings ranging from approximately 500 μm to approximately 3 mm.5. ...

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08-08-2013 дата публикации

Technology and method to study microbial growth and adhesion to host-related surfaces and the host-microbiota interaction

Номер: US20130203161A1
Принадлежит: Universiteit Gent

A method is provided for co-culturing viable cells and microorganisms for at least 48 hours in which an adhesion module is provided including a basal compartment and a luminal compartment separated by a semi-permeable membrane, and a continuous or semi-continuous flow of fresh medium is applied to the basal compartment.

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15-08-2013 дата публикации

Biomaterial handling device

Номер: US20130210123A1
Принадлежит: Jackson Laboratory

A biomaterial handling device is described that will provide for functionality for performing both IVF procedures and washing techniques in a single device. Disclosed embodiments provide increased protection to biomaterial samples during processing and handling. Embodiments of the invention reduce labor intensive processes for both IVF and washing treatments and address reduced risks of contamination of biological samples by providing an increasingly sterile environment.

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12-09-2013 дата публикации

V-Trough Photobioreactor System and Method of Use

Номер: US20130232866A1
Принадлежит: Heliae Development LLC

Disclosed herein are photobioreactor systems for high productivity aquaculture or aquafarming for growing of algae or other organisms in an aquatic environment featuring aspects that favor improved growth rates by achieving control over the contents of the growth medium, including carbon source, nitrogen source, and essential trace elements necessary for growth.

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03-10-2013 дата публикации

Culture systems

Номер: US20130260451A1
Автор: Dawn R. Applegate
Принадлежит: RegeneMed Inc

A microplate is provided herein having a plate body with at least one well formed therein, the well having a first open end, a second end, an aperture being formed in the second end, and a side wall extending between the first end and the second end. The microplate further has a permeable membrane extending at least partially across the aperture formed in the second end. A microplate is provided with a permeable membrane which allows not only for removal of certain solutes from a solution (high or low molecular weight solutes), but also allows for separation of macromolecular mixtures and degradation of the membrane. The microplate is also provided with an integrated top assembly or integrated inserts. Also provided herein are methods of culturing systems in the microplates described.

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03-10-2013 дата публикации

Culture systems

Номер: US20130260465A1
Автор: Dawn R. Applegate
Принадлежит: RegeneMed Inc

A microplate is provided herein having a plate body with at least one well formed therein, the well having a first open end, a second end, an aperture being formed in the second end, and a side wall extending between the first end and the second end. The microplate further has a permeable membrane extending at least partially across the aperture formed in the second end. A microplate is provided with a permeable membrane which allows not only for removal of certain solutes from a solution (high or low molecular weight solutes), but also allows for separation of macromolecular mixtures and degradation of the membrane. The microplate is also provided with an integrated top assembly or integrated inserts. Also provided herein are methods of culturing systems in the microplates described.

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26-12-2013 дата публикации

COMPOSITIONS COMPRISING NANOSTRUCTURES FOR CELL, TISSUE AND ARTIFICIAL ORGAN GROWTH, AND METHODS FOR MAKING AND USING SAME

Номер: US20130344600A1
Автор: Jin Sungho, OH Seunghan

The invention provides articles of manufacture comprising biocompatible nanostructures comprising nanotubes and nanopores for, e.g., organ, tissue and/or cell growth, e.g., for bone, kidney or liver growth, and uses thereof, e.g., for in vitro testing, in vivo implants, including their use in making and using artificial organs, and related therapeutics. The invention provides lock-in nanostructures comprising a plurality of nanopores or nanotubes, wherein the nanopore or nanotube entrance has a smaller diameter or size than the rest (the interior) of the nanopore or nanotube. The invention also provides dual structured biomaterial comprising micro- or macro-pores and nanopores. The invention provides biomaterials having a surface comprising a plurality of enlarged diameter nanopores and/or nanotubes. 118-. (canceled)19. A lock-in nanostructure comprising a plurality of nanopores or nanotubes , wherein the nanopore or nanotube entrance has a smaller diameter or size than the interior of the nanopore or nanotube to exhibit a re-entrant configuration.2019. The lock-in nanostructure of , wherein the nanopore or nanotube entrance has an average entrance diameter or average pore size by at least 10% to 50% smaller than the interior of the nanopore or nanotube dimension.2119. The lock-in nanostructure of , wherein the nanostructure comprises a Ti or a TiOin a nanopore or a nanotube configuration.22. A dual structured biomaterial comprising:(a) micro- or macro-pores, wherein the micro or macro pores has an average diameter, or equivalent diameter if the pores are not circular, in the range of between about 0.5-1,000 μm, or between about 1-100 μm(b) a surface area covered with nanotubes.23. The dual structured biomaterial of claim 22 , wherein the dual structured biomaterial comprises claim 22 , or is contained within claim 22 , an implant or an artificial organ or a joint.2427-. (canceled)28. The lock-in nanostructure of claim 19 , further comprising a plurality of cells.29 ...

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23-01-2014 дата публикации

Use of oxyhydrogen microorganisms for non-photosynthetic carbon capture and conversion of inorganic and/or c1 carbon sources into useful organic compounds

Номер: US20140024091A1
Автор: John S. Reed, Lisa Dyson
Принадлежит: Kiverdi Inc

Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or C1 carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing C1 chemicals including but not limited to various syngas compositions, into organic chemicals including biofuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or C1 carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO 2 in one or more steps of the process.

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06-03-2014 дата публикации

Methods for inactivating fluid cultures through heating

Номер: US20140065015A1
Принадлежит: Hyclone Laboratories LLC

A method for inactivating a fluid culture includes inserting a collapsible bag within a chamber of a tank assembly. A fluid is dispensed into a compartment of the collapsible bag, the fluid including a culture containing live cells or microorganisms. A lid is positioned over an opening of the tank assembly so that the collapsible bag is substantially enclosed within the tank assembly. The fluid within the collapsible bag is heated to an inactivation temperature that is sufficiently high to kill all of the live cells or microorganisms within the fluid. In one embodiment, the fluid within the flexible bag is mixed while the fluid is being heated to the inactivation temperature.

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13-03-2014 дата публикации

Methods of anaerobic digestion of biomass to produce biogas

Номер: US20140072994A1
Принадлежит: Novus Energy LLC

Improved methods for anaerobic digestion of organic matter to produce biogas. Among the improvements given are including ferric iron in a hydrolysis reactor to increase the rate and efficiency of anaerobic hydrolysis to provide substrates for methanogenesis. A solids separation step is added after hydrolysis and before methanogenesis to improve the efficiency of the methanogenesis step. Other improvements involve using separate tanks for the hydrolysis and methanogenesis stages and using two (or more) methanogenesis tanks in sequence, and switching the order of the two (or more) methanogenesis tanks periodically.

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20-03-2014 дата публикации

Bioreactor system for continuous cell cultivation

Номер: US20140080212A1
Автор: Soheil Asgari
Принадлежит: ATECH SYSTEM SOLUTIONS Ltd

A cell culture system for fermentation or cultivation of at least one of cells, tissues or tissue-like cell cultures, organs or organ-like cell cultures and multicellular organisms comprises: a system vessel ( 1 ) in which at least one cavity ( 6 ) is formed, the cavity ( 6 ) having at least three ports ( 2, 3, 4 ); at least one porous substrate ( 9 ) installed within the cavity ( 6 ) so as to allow fluid flowing from any one to any other of said three ports ( 2, 3, 4 ) to pass through said porous substrate ( 9 ); and, at least one channel extending from a first one of said ports ( 3 ) within the porous substrate ( 9 ) towards a central region of said porous substrate ( 9 ).

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06-01-2022 дата публикации

Gas Collection Device

Номер: US20220002648A1
Принадлежит: LG CHEM, LTD.

The present invention relates to a gas collection device and is directed to a gas collection device for collecting a gas which is generated while microorganisms are cultured in a super absorbent polymer product. The gas collection device may comprise a constant temperature chamber having an interior that is configured to be maintained at a set temperature; a culture flask unit located inside the constant temperature chamber and configured to culture a bacteria therein; an adsorption unit located outside the constant temperature chamber and configured to receive a gas inside the culture flask unit; a pump unit connected to a rear end of the adsorption unit and configured to suck the gas inside the culture flask unit into the adsorption unit; and a mass flow controller located outside the constant temperature chamber and configured to control a flow rate of the gas sucked into the adsorption unit. 1. A gas collection device comprising:a constant temperature chamber having an interior that is configured to be maintained at a set temperature;a culture flask unit located inside the constant temperature chamber and configured to culture bacteria therein;an adsorption unit located outside the constant temperature chamber and configured to receive a gas inside the culture flask unit;a pump unit connected to a rear end of the adsorption unit and configured to suck the gas inside the culture flask unit into the adsorption unit; anda mass flow controller located outside the constant temperature chamber and configured to control a flow rate of the gas sucked into the adsorption unit.2. The gas collection device of claim 1 , wherein the mass flow controller is located at a rear end of the culture flask unit and at a front end of the adsorption unit claim 1 , and the mass flow controller is configured to control the flow rate of the gas flowing from the culture flask unit to the adsorption unit.3. The gas collection device of claim 2 , further comprising:an injection flow path ...

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06-01-2022 дата публикации

MICROORGANISM CULTURE APPARATUS AND MICROORGANISM CULTURE METHOD

Номер: US20220002650A1
Принадлежит:

A microorganism culture apparatus includes a three-layer stacked structure having a layered culture unit () that cultures a microorganism, a layered nutrient supply unit () that is arranged on a first surface () of the culture unit () and supplies a nutrient to the culture unit (), and a layered environmental component supply unit () that is arranged on a second surface () and supplies an environmental component to the culture unit (). 1. A microorganism culture apparatus comprising a three-layer stacked structure that includes:a layered culture unit that cultures a microorganism; andat least one of a layered nutrient supply unit that supplies a nutrient to the culture unit and a layered environmental component supply unit that supplies an environmental component to the culture unit, the layered nutrient supply unit and the layered environmental component supply unit being arranged on a first surface and a second surface opposite to the first surface of the culture unit.2. The microorganism culture apparatus according to claim 1 , wherein in the three-layer stacked structure claim 1 , the nutrient supply unit is arranged on the first surface of the culture unit claim 1 , and the environmental component supply unit is arranged on the second surface.3. The microorganism culture apparatus according to claim 1 , wherein in the three-layer stacked structure claim 1 , the nutrient supply unit is arranged on the first surface and the second surface of the culture unit.4. The microorganism culture apparatus according to claim 3 , wherein the nutrient supply unit arranged on the first surface and the nutrient supply unit arranged on the second surface are different from each other in at least one of a type and a concentration of a nutrient to be supplied.5. The microorganism culture apparatus according to claim 1 , wherein in the three-layer stacked structure claim 1 , the environmental component supply unit is arranged on the first surface and the second surface of the ...

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07-01-2016 дата публикации

SCREW CAP LIDDED CONTAINER

Номер: US20160001931A1
Автор: Seippel Martin
Принадлежит:

A screw cap lidded container for laboratory use includes a container body defining a chamber, a tubular section of the container body that is connected at the rear to the chamber and has a container opening in the front and an external thread on the outer periphery of the tubular section. There is a circumferential first sealing surface on the tubular section and at least one first projection, which projects from the container body at a peripheral position of the tubular section, and a screw cap having an internal thread on the inner periphery that can be screwed onto the external thread with thread play, a second sealing surface on the screw cap can be brought into sealing contact with the first sealing surface, and at least one second projection, which projects at a peripheral position of the screw cap. 1. A screw cap lidded container for laboratory use comprising:{'b': 2', '23, 'a container body () defining a chamber (),'}{'b': 14', '2', '23', '21, 'a tubular section () of the container body () that is connected at the rear to the chamber () and has a container opening () in the front,'}{'b': 16', '14, 'an external thread () on the outer periphery of the tubular section (),'}{'b': 22', '14, 'a circumferential first sealing surface () on the tubular section (),'}{'b': 19', '20', '2', '14, 'at least one first projection (, ), which projects from the container body () at a peripheral position of the tubular section (),'}{'b': '26', 'a screw cap (),'}{'b': 34', '33', '26', '16, 'an internal thread () on the inner periphery () of the screw cap () that can be screwed onto the external thread () with thread play,'}{'b': 36', '26', '26', '14', '22, 'a circumferential second sealing surface () on the screw cap (), that by screwing the screw cap () tightly onto the tubular section () can be brought into sealing contact with the first sealing surface (), and'}{'b': 38', '39', '26', '26, 'at least one second projection (, ), which projects from the screw cap () at a ...

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07-01-2016 дата публикации

Structure for culturing cells

Номер: US20160002583A1
Принадлежит: DDNT CONSULTANTS AUSTRALIA Pty Ltd

The present invention relates to a structure and a container for culturing cells. In one arrangement, the structure ( 200 ) comprises a partially enclosed cavity ( 204 ) and a plurality of compartments ( 201 ) adapted for fluid communication with the partially enclosed cavity ( 204 ). Each compartment ( 201 ) is configured to hold one or more cell within its compartment ( 201 ) at a location dependent on a dimension of the cell. This arrangement may facilitate visual identification or indication of the size of the cell by observing its location within its compartment ( 201 ). The structure ( 200 ) may be included or formed integrally with a container ( 100 ) such as a Petri dish.

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07-01-2016 дата публикации

SOLAR STEAM PROCESSING OF BIOFUEL FEEDSTOCK AND SOLAR DISTILLATION OF BIOFUELS

Номер: US20160002673A1
Принадлежит: William Marsh Rice University

A method of producing bioethanol that includes receiving a feedstock solution that includes polysaccharides in a vessel comprising a complex is described. The complex may be copper nanoparticles, copper oxide nanoparticles, nanoshells, nanorods, carbon moieties, encapsulated nanoshells, encapsulated nanoparticles, and/or branched nanostructures. The method also includes applying electromagnetic (EM) radiation to the complex such that the complex absorbs the EM radiation to generate heat. Using the heat generated by the complex, sugar molecules may be extracted from the polysaccharides in the feedstock solution, and fermented. Then, bioethanol may be extracted from the vessel. 1. A method of producing bioethanol , the method comprising:receiving, in a vessel comprising a complex, a feedstock solution comprising polysaccharides, wherein the complex is a least one selected from a group consisting of copper nanoparticles, copper oxide nanoparticles, nanoshells, nanorods, carbon moieties, encapsulated nanoshells, encapsulated nanoparticles, and branched nanostructures;applying electromagnetic (EM) radiation to the complex, wherein the complex absorbs the EM radiation to generate heat;extracting, using the heat generated by the complex, sugar molecules from the polysaccharides in the feedstock solution; andfermenting the sugars molecules to generate bioethanol; andextracting the bioethanol from the vessel.2. The method of claim 1 , wherein extracting the bioethanol from the vessel comprises:condensing, using a condenser, the bioethanol from the vessel; andstoring the bioethanol in a storage tank.3. The method of claim 1 , wherein extracting the bioethanol from the vessel comprises:applying additional EM radiation to the complex, wherein the complex absorbs the additional EM radiation to generate additional heat;transforming, using the additional heat generated by the complex, the bioethanol to a vapor; andextracting the vapor from the vessel.4. The method of claim 1 , ...

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04-01-2018 дата публикации

Bioreactor System for Cell Cultivation

Номер: US20180002655A1
Принадлежит: General Electric Co

The present invention relates to a novel bioreactor system for cell cultivation. More specifically, the invention relates to a compact bioreactor system which has several integrated functions and enables small scale static culture as well as scale-up rocking culture in the same bioreactor. The bioreactor system comprises tray for positioning of a cell culture bag having adjustable volume, a lid covering the cell culture bag and provided with heating function, an integrated perfusion unit, an integrated cell loading unit, and an integrated unit for automatic cell culture sampling, wherein the bioreactor system is controlled by a single control unit. The invention also relates to a method of cell culture using the bioreactor system for culture of therapeutic cells.

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07-01-2021 дата публикации

Culture Vessel and Cell Culture Device

Номер: US20210002600A1
Принадлежит: HITACHI LTD, Sumitomo Bakelite Co Ltd

A culture vessel that can suppress foaming in a culture medium when a common nozzle is used to supply a liquid culture medium and a gas together. This culture vessel includes: a vessel body having an opening portion that communicates with a housing space; a lid that closes the opening portion; and a nozzle that passes through the and extends to the housing space, and supplies a liquid culture medium and a gas together. The nozzle has a vent opening portion that is formed so as to open in a side of the nozzle and serves as a movement path of the gas from inside of the nozzle into the housing space.

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03-01-2019 дата публикации

DROPLET FLUID CONNECTIONS

Номер: US20190002811A1
Принадлежит:

Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly. 1. A first fluidic device comprising a substrate having a first mating surface , said first mating surface comprising one or more fluidic ports , wherein said first mating surface is adapted to stably retain a first protruding liquid droplets comprising a first liquid at the one or more fluidic ports , wherein said port of said first fluidic device comprising said first protruding liquid droplet is aligned with a port of a second fluidic device comprising a second protruding droplet so as to permit fluidic communication.2. The fluidic device of claim 1 , wherein said first mating surface comprises one or more regions surrounding the one or more fluidic ports claim 1 , and wherein said regions are adapted to resist wetting by said first liquid.3. The fluidic device of claim 2 , wherein said regions are adapted to be hydrophobic.4. The fluidic device of claim 2 , wherein said one or more regions comprise a first material selected to resist wetting by said first liquid.5. The fluidic device of claim 4 , wherein the first material is selected from the group consisting of poly-tetrafluoroethylene (PTFE) claim 4 , a perfluoroalkoxy alkane (PFA) claim 4 , fluorinated ethylenepropylene (FEP) claim 4 , polydimethylsiloxane (PDMS) claim 4 , nylon claim 4 , polypropylene claim 4 , polystyrene and polyimide.6. The fluidic device of claim 4 , wherein the substrate comprises said first material.7. The fluidic device of claim 4 , wherein said first material is bonded claim 4 , adhered claim 4 , coated or sputtered onto said first surface.8. The fluidic device of claim 4 , wherein said first material comprises a hydrophobic gasket.9. The fluidic device of claim 2 , wherein the one or more regions are ...

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03-01-2019 дата публикации

Large-scale Bioreactor

Номер: US20190002815A1
Принадлежит: 3D Biotek, LLC

In an embodiment of the invention, there may be provided a bioreactor having tissue scaffolds and having culture medium perfused therethrough. There may be multiple independent culture chambers and reservoirs or sub-reservoirs. Sensors can provide for individually controlling conditions in various culture chambers, and various culture chambers can be operated differently or for different durations. It is possible to infer the number of cells or the progress toward confluence from the fluid resistance of the scaffold, based on flowrate and pressure drop. Harvesting may include any combination or sequence of; exposure to harvesting reagent; vibration; liquid flow that is steady, pulsatile or oscillating; passage of gas-liquid interface through the scaffold. Vibration and flow can be applied so as to reinforce each other. 1. A bioreactor system for culturing cells ,said bioreactor system comprising spatially fixed scaffolds upon which said cells can grow,said bioreactor system having a liquid supply system for perfusing liquid through said scaffolds,wherein said bioreactor system comprises a plurality of culture chambers each containing some of said scaffolds, said culture chambers having respective flow paths therethrough for flow of said liquid,wherein said bioreactor system comprises a plurality of reservoirs or a plurality of sub-reservoirs,wherein said bioreactor system has a control device to direct, to various of said plurality of culture chambers at a given time, respective flows of said liquid that are different from flows to others of said culture chambers with respect to flowrate of said liquid or flow direction of said liquid or duration of flow of said liquid.2. The bioreactor system of claim 1 , wherein said control device comprises at least one sensing device selected from the group consisting of a pH sensor claim 1 , a dissolved oxygen sensor claim 1 , a glucose sensor claim 1 , a lactate sensor claim 1 , a camera claim 1 , and a device indicating a ...

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13-01-2022 дата публикации

BIOLOGICAL FLUIDIC SYSTEM

Номер: US20220010259A1

Provided is a biological fluidic system that provides a high degree of sterilization by performing the biological processing in a closed system in which the fluid is contained in chambers that are isolated from the environment in a manner that does not permit ingress of contaminants. 1. A system for cultivating microorganisms or performing biological processing , comprising:a fluid driving mechanism; and a processing chamber,', 'a duct extending between two ports of the chamber to permit flow of fluid between the two ports through the duct, and', 'a flow-driving region defined within the duct, flow-driving region is configured for engagement with the fluid driving mechanism to thereby propel fluid flow between the two ports in response to selective pressure on different pliable regions of the flow-driving region., 'a processing arrangement formed between two pliable polymeric sheets, the processing arrangement comprising2. The system of claim 1 , wherein the flow-driving region is generally circular and the fluid driving mechanism is configured to peristaltically propel the fluid.3. The system of claim 1 , wherein the driving mechanism comprises a driving motor with a magnetic element configured for magnetic coupling with an engaging element that is engaged with and configured to propel fluid through the flow-driving region.4. The system claim 1 , comprising a venting arrangement permitting gas exchange between the interior of the processing arrangement and the exterior.5. The system of claim 4 , wherein the venting arrangement is configured to permit gas exchange without passage of microorganism therethrough.6. The system of claim 4 , wherein the venting arrangement comprises a filter for filtering solid particles.7. The system of claim 1 , comprising an inoculation port for introducing living matter into the chamber.8. The system of claim 1 , wherein opposite walls of the processing chamber are fixed to one another in one or more regions other than the edges of ...

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20-01-2022 дата публикации

Devices and Methods for Inoculating a Target

Номер: US20220017848A1
Принадлежит: STRATIX LABS CORPORATION

Inoculating systems/devices, methods for inoculating a target, and coating methods are disclosed. An example inoculating system may include an inoculating member having a transfer region and a handle region. A pre-determined quantity of viable microorganisms may be disposed on the transfer region. The inoculating member may be configured to transfer the pre-determined quantity of viable microorganisms to a target during an inoculation operation without having to rehydrate the pre-determined quantity of viable microorganisms prior to the inoculating operation. 1. An inoculating system , comprising:an inoculating member having a transfer region and a handle region;a pre-determined quantity of viable microorganisms disposed on the transfer region; andwherein the inoculating member is configured to transfer the pre-determined quantity of viable microorganisms to a target during an inoculation operation without having to rehydrate the pre-determined quantity of viable microorganisms prior to the inoculating operation.2. The inoculating system of claim 1 , further comprising a cap removably disposed over the transfer region.3. The inoculating system of claim 1 , wherein the transfer region includes a rounded peripheral surface.4. The inoculating system of claim 1 , wherein the pre-determined quantity of microorganism includes 1 to 1000 colony forming units of viable microorganisms.5. The inoculating system of claim 1 , wherein the transfer region is configured to transfer 50% or more of the pre-determined quantity of viable microorganisms to the target.6. The inoculating system of claim 1 , wherein the target has a water content of 5% or more.7. The inoculating system of claim 1 , wherein the target includes agar media.8. The inoculating system of claim 1 , wherein the target includes a liquid.9. The inoculating system of claim 1 , wherein the target includes a microbial collection device.10. The inoculating system of claim 1 , wherein a plurality of projections are ...

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20-01-2022 дата публикации

SELF-CONTAINED BIOLOGICAL INDICATOR

Номер: US20220017849A1
Принадлежит: Mesa Laboratories, Inc.

A SCBI useful in ambient air pressure systems is disclosed. The SCBI includes a body which serves as the culture tube, a glass media ampoule, an inoculated stainless steel disc positioned on the top of the glass media ampoule so that the spores are close to the top/opening of the SCBI, filter paper on the top of the body and overlying the disc, and a cap. 1. A method of determining the accuracy of a sterilization or decontamination process in a sterilization chamber comprising the steps of:placing a self-contained biological indicator (SCBI) in said sterilization chamber in an open position wherein a sterilant gas will enter said SCBI;said SCBI comprising a body adapted to serve as a culture tube, said body having a cylindrical side wall which does not contain any recessed areas, a bottom wall at one end of the cylindrical side wall closing the body, a collar at the other end of the cylindrical side wall extending outwardly from the cylindrical side wall having an open end having an interior diameter greater than an interior diameter of the cylindrical side wall and adapted to receive a cap, said collar including a plurality of inwardly extending ribs; a cap adapted to engage said body in an open position and a closed position, said cap including a top wall and a cylindrical side wall adapted to engage said collar of said body, said cylindrical side wall including a plurality of apertures; a rupturable glass ampoule including a growth media in said body; and a stainless steel disc inoculated with spores on top of said glass ampoule and adjacent said plurality of ribs;subjecting said sterilization chamber to said sterilant gas;removing said SCBI from said sterilization chamber;rupturing said glass ampoule in said SCBI to release said growth media to said body of said SCBI, wherein said stainless steel disc is adapted to drop down into said cylindrical side wall of said body to contact said inoculated spores with said growth media; andclosing said SCBI and determining ...

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14-01-2016 дата публикации

CONTROLLED GROWTH ENVIRONMENTS FOR ALGAE CULTIVATION

Номер: US20160007549A1
Автор: Oyler James R.
Принадлежит:

A method for cultivating algae can include providing a body of water in a substantially enclosed system. The enclosed system can have a length of channel and a cover. The method can optionally include circulating the body of water through the enclosed system under positive pressure conditions. The positive pressure should prevent ingress of any external atmosphere or material. Further, the method can include cultivating the algae in the body of water at conditions which promote growth. Likewise, a system for cultivating algae can include a channel with a cover, water in the channel, and a pump to introduce positive pressure into the system. 1. A system for cultivating algae , comprising:a channel with a cover system that substantially closes the channel to an outside environment and forms a protected growth volume, the cover system being designed to selectively adjust amounts of light allowed to enter the protected growth volume;a body of water including one or more algae contained in the channel; anda gas pump configured to introduce a positive pressure into the protected growth volume.2. The system of claim 1 , wherein the cover system includes a first cover and a second cover claim 1 , such that the first cover has a greater translucence than the second cover.3. The system of claim 2 , wherein the first cover and the second cover are configured to cover different portions of the channel.4. The system of claim 2 , wherein the second cover is configured to removably cover at least a portion of the channel and the first cover.5. The system of claim 2 , wherein at least a portion of the second cover is opaque or substantially opaque sufficient to block light to promote heterotrophic growth of the algae.6. The system of claim 2 , wherein at least one of the first and second covers includes perforations.7. The system of claim 1 , further comprising a reflecting layer on a portion of an inner wall of the channel within the protected growth volume claim 1 , said ...

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12-01-2017 дата публикации

INCUBATOR APPARATUS AND METHODS

Номер: US20170009195A1
Принадлежит: Unisense FertiliTech A/S

Apparatus and methods for monitoring embryos in an incubator are described. The apparatus comprises an incubation chamber defined by an incubation chamber housing and a slide carrier comprising a plurality of compartment walls that define compartments for holding embryo slides within the incubation chamber for incubation. The slide carrier is moveable, for example by rotation, relative to the incubation chamber housing to allow a selected compartment to be moved to a loading position defined at least in part by a loading port wall associated with the incubation chamber housing. The loading port wall is arranged to cooperate with the wall of a compartment in the loading position to restrict the extent to which the environment of the compartment in the loading position is in fluid communication with the environments of other compartments in the incubation chamber. 1. An incubator for embryos supported on embryo slides , the incubator comprising:an incubation chamber defined by an incubation chamber housing; anda slide carrier comprising a plurality of compartment walls that define compartments for holding embryo slides within the incubation chamber for incubation, wherein the slide carrier is moveable relative to the incubation chamber housing to allow a selected compartment to be moved to a loading position defined at least in part by a loading port wall associated with the incubation chamber housing, and wherein the loading port wall is arranged to cooperate with the wall of a compartment in the loading position to restrict the extent to which the environment of the compartment in the loading position is in fluid communication with the environments of other compartments in the incubation chamber.2. The incubator of claim 1 , wherein the loading port wall is positioned to align with the compartment wall of a compartment in the loading position to restrict the extent to which the environment of the compartment in the loading position is in fluid communication with the ...

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12-01-2017 дата публикации

Cell culture and experiment device

Номер: US20170009196A1

The invention discloses a cell culture and experiment device used in the field of biological and genetic engineering experiment apparatus, comprising a central distribution compartment, a culture compartment, a treatment compartment, and pipelines for delivering liquid between the central distribution compartment and the culture compartment and between the central distribution compartment and the treatment compartment. The central distribution compartment is equipped with a distribution chamber and a piston which can be moved forward and backward in the distribution chamber to alter the working volume of the distribution chamber. At the bottom of the distribution chamber, the central distribution compartment is equipped with a distribution valve controlling the connectivity between the distribution chamber and any of the channels. The invention provides a miniaturized apparatus integrating the central distribution compartment, the culture compartment and the treatment compartment, which can replace manual operations, save time and labor, and avoid wasting experimental raw material.

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10-01-2019 дата публикации

CELL AND TISSUE CULTURE CONTAINER

Номер: US20190010439A1
Автор: Blahut Eric
Принадлежит: HISTOGENICS CORPORATION

The invention generally relates to containers for cell and tissue culturing with multiple compartments in fluid communication with each other to provide a common culture environment in each of the compartments while maintaining physical separation of cells and tissue therein. The invention further relates to culture containers providing a sterile culture environment with detachably coupleable lids and open access to each compartment within a container. 1. A method for culturing a tissue comprising:providing a container comprising a bottom wall coupled to a bottom edge of at least one side wall, and an at least one interior wall coupled to the at least one side wall to define at least a first compartment and a second compartment within the container, wherein the at least one interior wall comprises a bottom edge that does not contact the bottom wall, leaving a gap between the bottom wall and the at least one interior wall, wherein the gap allows fluid to pass between the first and second compartments without allowing cells, tissues, or 3D scaffolds to pass therebetween;providing a lid comprising a gas-permeable membrane, wherein the lid is configured to be detachably coupled to a top edge of the at least one side wall;introducing a first living cell of a first cell type to the first compartment and a second living cell of a second cell type to the second compartment in the presence of a cell culture medium;exposing the container to conditions promoting cell growth to produce a first tissue from the first living cell and a second tissue from the second living cell.2. The method of claim 1 , wherein the first cell type or the second cell type is a chondrocyte claim 1 , mesenchymal stem cell claim 1 , fibroblast claim 1 , osteocyte claim 1 , osteoblast claim 1 , synoviocyte claim 1 , induced pluripotent stem cell (iPSC) claim 1 , embryonic stem cell (ESC) claim 1 , lymphocyte claim 1 , adipocyte claim 1 , myofibroblast claim 1 , hepatocyte claim 1 , islet cell claim 1 , ...

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03-02-2022 дата публикации

Multicompartment bag for cultivation of cells

Номер: US20220033748A1
Принадлежит: Global Life Sciences Solutions USA LLC

The invention discloses a flexible plastic bag for cultivation of cells, including a top wall film and a bottom wall film, each having an inside and an outside, sealed to each other inside-to-inside by durable weld seams, optionally via one or more side wall films, to form a bag with an inner volume delimited by the durable weld seams; one or more ports through the top and/or bottom wall film for introduction and withdrawal of fluids; one or more frangible weld seams joining the insides of said top and bottom wall films, dividing the bag inner volume into a plurality of cultivation compartments; and one or more gripping means affixed to the top and bottom wall films, adjacent each of the frangible weld seams and adapted to break a specific frangible weld seam by pulling apart the gripping means on the top and bottom wall films.

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03-02-2022 дата публикации

Bioreactor for cell culture

Номер: US20220033752A1
Принадлежит: Individual

A bioreactor for cell culture comprising, a first set of open top troughs that are interconnected by a first set off low channels to form a first cascade, a second set of open top troughs that are interconnected by a second set of flow channels to form a second cascade, and a set of interconnecting flow channels coupled between the first cascade and the second cascade, wherein the first cascade circulates a fresh media and the second cascade circulates a cell culture and a first quantity of the fresh media from first cascade is supplied to the second cascade through the set of interconnecting flow channels when a second quantity of cell culture is removed from the second cascade.

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03-02-2022 дата публикации

OPEN-TOP MICROFLUIDIC DEVICE WITH STRUCTURAL ANCHORS

Номер: US20220033757A1
Принадлежит:

A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers. 146-. (canceled)47. A microfluidic device , comprising i) a chamber with a lumen and a fluidic cover comprising a microfluidic channel , ii) a membrane below said chamber , wherein said fluidic cover is configured to removably cover and close said chamber.48. The microfluidic device of claim 47 , wherein said membrane is a porous membrane.49. The microfluidic device of claim 47 , wherein said chamber comprises cells.50. The microfluidic device of claim 47 , wherein said chamber comprises a gel.51. The microfluidic device of claim 47 , wherein said chamber is configured for fluid flow.52. The microfluidic device of claim 47 , wherein said chamber is circular.5360-. (canceled) This application is a Divisional of, and claims priority to, co-pending U.S. patent application Ser. No. 17/160,617, filed Jan. 28, 2021, which is a Continuation of application Ser. No. 15/781,370 filed Jun. 4, 2018, now U.S. Pat. No. 10,961,496 issued Mar. 30, 2021, which is a 371 of International PCT/US16/64814 filed Dec. 2, 2016, now expired, which claims priority to Provisional Ser. No. 62/263,492 filed Dec. 4, 2015, now expired, the contents of which are incorporated herein in their entirety.A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s).In vitro models involving thick gels, i.e. >0.2 mm in thickness (and more typically >0.5 mm in thickness), have a common problem of shrinkage due to evaporation of water and ...

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03-02-2022 дата публикации

INTEGRATED CELL MONITORING APPARATUS AND METHOD OF USING THE SAME

Номер: US20220033759A1

Cell monitoring apparatus includes sensing chip and channel module. Sensing chip includes channel region, source and drain regions, and sensing film. The channel region includes first semiconductor material. The source and drain regions are disposed at opposite sides of the channel region, and include a second semiconductor material. Sensing film is disposed on the channel region at a sensing surface of the sensing chip. Channel module is disposed on the sensing surface of sensing chip. A microfluidic channel is formed between the sensing surface of the sensing chip and a proximal surface of the channel module. The microfluidic channel includes a culture chamber and a micro-well. The culture chamber is concave into the proximal surface of the channel module, and overlies the channel region. The micro-well is concave into a side of the culture chamber, and directly faces the sensing film. 1. A cell monitoring apparatus , comprising: a channel region comprising a first semiconductor material;', 'a pair of source and drain regions disposed at opposite sides of the channel region and comprising a second semiconductor material; and', 'a sensing film disposed on the channel region at a sensing surface of the sensing chip; and, 'a sensing chip, comprisinga channel module, disposed on the sensing surface of the sensing chip, a culture chamber, concave into the proximal surface of the channel module, at a region of the proximal surface of the channel module facing the channel region; and', 'a micro-well, concave into a side of the culture chamber, wherein the micro-well directly faces the sensing film., 'wherein a microfluidic channel is formed between the sensing surface of the sensing chip and a surface of the channel module proximal to the sensing chip, the microfluidic channel comprising2. The cell monitoring apparatus of claim 1 , wherein the channel module comprises:a first region of a polymeric material having a first thickness, wherein the first region extends from ...

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15-01-2015 дата публикации

DEVICE AND METHOD FOR BACTERIAL CULTURE AND ASSAY

Номер: US20150017629A1
Принадлежит:

The present invention provides a simple culture device that is designed for manufacture and use in areas of limited resources. The device is useful for cell culture in such environments with limited resources because cells grow in paper just as they do in a culture dish. Also provided is a binding assay that employs an activatable dormant bacteriophage carrying a reporter gene to qualitatively or quantitatively detect the presence of a substance of interest in a sample. 1. A portable culture device comprising:a) a first culture strip comprising at least one culture region within a first strip of fluid impermeable material having an adhesive on its inner surface;b) a second culture strip comprising at least one culture region within a second strip of fluid impermeable material having an adhesive on its inner surface, wherein each of the at least one culture regions of the second culture strip corresponds to one of the at least one culture regions of first culture strip to form a culture region pair,wherein each culture region comprises an inner layer of porous hydrophilic material and at least one culture region in each culture region pair comprises an outer layer of a gas permeable membrane, andwherein the device is moveable between an open condition and a closed condition in which the first and second culture strips are releasably adhered via the inner adhesive surfaces such that the culture regions in each culture pair are aligned and the outer gas permeable membrane or gas permeable membranes are exposed to the environment to permit gas flow to the interior of the device.2. The device of claim 1 , wherein at least one of the culture regions comprises an adsorbent pad layered on the side of the porous hydrophilic material opposite the interior of the device.3. The device of claim 2 , wherein a dialysis membrane is layered between the adsorbent pad and the porous hydrophilic material.4. The device of claim 1 , wherein the first culture strip is connected to the ...

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15-01-2015 дата публикации

Stacked Membrane Bioreactor

Номер: US20150017683A1
Принадлежит:

Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described. 1. A bioreactor , comprising:a biomaterial reactor chamber;a gas reactor chamber in fluid communication with a first side of the biomaterial reactor chamber;a medium reactor chamber in fluid communication with a second side of the biomaterial reactor chamber;a gas inlet and a gas outlet in fluid communication with the gas reactor chamber;a medium inlet and a medium outlet in fluid communication with the medium reactor chamber; and a flame arrestor, the flame arrestor being located in one or more of: the gas reactor chamber, the gas inlet, the gas outlet, the medium reactor chamber, the medium inlet, and the medium outlet; and', 'a rigid foam comprised by the gas reactor chamber., 'one or more of23-. (canceled)43. The bioreactor of claim , the biomaterial reactor chamber comprising a living organism capable of using hydrogen gas as a source of energy.5. (canceled)6Ralstonia eutropha.. The bioreactor of claim 1 , the biomaterial reactor chamber comprising7. The bioreactor of claim 1 , wherein the biomaterial reactor chamber includes a membrane assembly claim 1 , the membrane assembly comprising one or more of:a gas permeable membrane comprising one or more of: silicone, polyvinylidene difluoride (PVDF), porous polytetrafluoroethylene (PTFE), acrylic copolymer, polycarbonate, polypropylene, and modified nylon;a liquid permeable membrane comprising one or more of: woven material, cloth, mesh, glass fibers, nitrocellulose, mixed cellulose ester (MCE), polycarbonate, modified polycarbonate, polyethersulfone (PES), nylon, ceramic, PTFE, ...

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18-01-2018 дата публикации

SHAKE-TYPE CULTURE APPARATUS AND CULTURE METHOD USING THE SAME

Номер: US20180016534A1
Автор: Matsuda Hiroyuki
Принадлежит:

The present invention provides a shake-type culture apparatus which can perform large scale culture without using stirring blades and a culture method using the same. More particularly, there are provided a shake-type culture apparatus comprising: a culture bag made from a soft packaging material; an outer shell container being arranged to house the entirety of the culture bag to be able to perform adjustment of a temperature thereof; and a power source for shaking the outer shell container housing the culture bag, wherein the contents in the culture bag can be stirred and mixed by shaking the outer shell container housing the culture bag; and a culture method using the same. 2. The shake-type culture apparatus according to claim 1 , wherein said outer shell container housing said culture bag rotates in a horizontal direction.3. The shake-type culture apparatus according to claim 1 , wherein said outer shell container housing said culture bag has a rotation number of shaking of 0.1 to 2 claim 1 ,000 rpm and an amplitude of 0.1 to 100 mm.4. The shake-type culture apparatus according to claim 1 , wherein the shake-type culture apparatus comprises a device for detecting a height of a wave of said contents in said culture bag claim 1 , and is provided with an adjustment mechanism for adjusting the rotation number of shaking of said outer shell container housing said culture bag so that the height of the wave of said contents becomes constant.5. The shake-type culture apparatus according to claim 1 , wherein said outer shell container comprises a jacket structure through which circulating water can be passed or a rubber heater.6. The shake-type culture apparatus according to claim 1 , wherein said culture bag is a gazette bag claim 1 , and is provided with a spout.7. The shake-type culture apparatus according to claim 1 , wherein the shake-type culture apparatus is provided with a sensor for measuring a pH or a dissolved oxygen concentration (DO) of the contents in said ...

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18-01-2018 дата публикации

APPARATUS, SYSTEMS AND METHODS FOR PROGRAMMABLE TISSUE CULTURE ILLUMINATION

Номер: US20180016538A1
Автор: Bugaj Lukasz, Lim Wendell
Принадлежит:

The disclosed apparatus, systems and methods relate to an illumination opto-plate configured to specifically light the wells of a culture plate. 1. An illumination device , comprising: i. a plurality of LED fixtures;', 'ii. a microcontroller; and', 'iii. at least one LED driver; and, 'a. a base, comprising;'} i. at least one layer;', 'ii. a plurality of openings; and', 'iii. a plurality of walls,, 'b. an adaptor, comprising;'}wherein the isled fixtures are configured to illuminate a culture plate.2. The illumination device of claim 1 , wherein each of the plurality of LED fixtures is independently programmable.3. The illumination device of claim 1 , wherein the adaptor has three layers.4. The illumination device of claim 3 , wherein a plurality of diffuser paper is disposed between the adaptor layers.5. The illumination device of claim 3 , wherein the adaptor layers further comprise mounting points.6. The illumination device of claim 3 , wherein the adaptor further comprises a mounting ridge configured for reception of the culture plate.7. The illumination device of claim 6 , wherein the walls of the adaptor are configured for individual well illumination.8. The illumination device of claim 7 , wherein the opto-plate further comprises a memory.9. A system for illuminating a culture plate comprising: i. at least one LED fixture;', 'ii. at least one LED driver; and', 'iii. at least one microcontroller;, 'a. an opto-plate, comprising i. at least one layer;', 'ii. at least one opening; and', 'iii. a plurality of walls, wherein the adaptor is attached to the opto-plate such that when the culture plate is placed on the adaptor the LED fixtures illuminate the culture plate., 'b. an adaptor, comprising;'}10. The system of claim 9 , wherein the walls of the adaptor are configured such that each well within the culture plate is individually illuminated.11. The system of claim 9 , further comprising a lid.12. The system of claim 9 , further comprising an Arduino configured to ...

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21-01-2021 дата публикации

Photobioreactor with annular chambers

Номер: US20210017480A1
Автор: Narasimhan Sundaram
Принадлежит: ExxonMobil Research and Engineering Co

A photobioreactor includes one or more annular chambers concentrically positioned about a central axis, and an algae slurry contained within the one or more annular chambers.

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16-01-2020 дата публикации

Well-plate incubator

Номер: US20200017817A1
Принадлежит: Berkeley Lights Inc

Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.

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21-01-2021 дата публикации

Auto-aligning cable sensor

Номер: US20210018523A1
Принадлежит: Automation Partnership Cambridge Ltd

An auto-aligning cable sensor for performing measurements on a medium contained in a vessel is provided. The cable sensor includes a cable which has an end sensor and which is adapted to be carried by a mounting fixture. The mounting fixture and the vessel are movable into a measurement position relative to each other such that a contact face of the end sensor is held in alignment against a surface of the vessel by a pressing force, thereby enabling the end sensor to sense a characteristic of the medium contained in the vessel. The cable sensor further includes an elastically deformable coupling member which is arranged to mediate transfer of the pressing force from the mounting fixture to the cable and thence to the end sensor when the mounting fixture and the vessel are moved into the measurement position. The coupling member deforms under the pressing force such that the cable can flex to tilt the end sensor relative to the surface of the vessel to accommodate misalignment of the contact face and the surface of the vessel.

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28-01-2016 дата публикации

ROTATIONAL DUAL CHAMBER BIOREACTOR: METHODS AND USES THEREOF

Номер: US20160024453A1
Принадлежит:

A rotational dual chamber bioreactor for cell culture in bi- and multilayered scaffolds, in the context of tissue engineering (TE) and regenerative medicine strategies is disclosed which comprises at least two dual culture chambers, a multiposition magnetic stirrer plate, and at least two flow pumps which can be coupled to a monitor device to register biochemical parameters such as pH, pO2, glucose and urea sensors and physical parameters like pressure. The dual culture chambers have a central barrier with a hole to insert the bilayer scaffold, such chamber is used to culture cells with different conditions in each compartment of the chamber, avoiding medium exchange. Each chamber can rotate 180° (horizontal), being the rotations per minute controlled independently. The rotation is promoted by magnetic stirrers, having magnets performing the attraction between the bottom part of the dual chamber and the stirring position of the plate. The multiposition stirrer plate is adapted dimensionally to the requirements of the culture plate dimensions used, and also allows 180° vertical rotation. Each dual chamber has detachable caps for the top and the bottom chambers. The top cap has the possibly to compress the scaffold and thus providing a compressive stimulus with torsion simultaneously. This compression is applied by attaching a top plate adapted to fit the top caps of each dual chamber. 1. A bioreactor comprising a multiposition magnetic stirred plate on which are placed at least two dual chambers , a top plate fitting the top cap of each dual chamber and at least two independent flow pumps.2. The bioreactor according to claim 1 , wherein the dual chambers are fixed to the stirred plate by magnetic attraction.3. The bioreactor according to claim 1 , wherein the stirred plate has a rotational movement.4. The bioreactor according to claim 3 , wherein the rotational movement is horizontal and/or vertical.5. The bioreactor according to claim 4 , wherein the horizontal ...

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26-01-2017 дата публикации

RADIAL MICROFLUIDIC DEVICES AND METHODS OF USE

Номер: US20170022464A1
Принадлежит:

A microfluidic device for simulating a function or response of a tissue is disclosed. The device includes an inlet for receiving a fluid in the device, and an outlet for removing the fluid from the device. The device further includes a fluid channel in fluid communication with the inlet and the outlet for flowing the fluid through the device. The fluid channel defines a chamber well that receives cells associated with the tissue. The device also includes an interface structure between the fluid channel and the chamber well for permitting migration of at least one of cells, particulates, chemicals, molecules, liquids, or gases between the fluid within the fluid channel and the chamber well. 1105-. (canceled)106. A microfluidic device comprising:i) a chamber, said chamber defined by one or more walls and comprising a lumen, said lumen comprising one or more geometric features; andii) a gel confined by said one or more geometric features, said gel comprising cells, said cells in contact with fluid from one or more iii) fluidic channels.107. The device of claim 106 , wherein said matrix comprises collagen.108. The device of claim 106 , further comprising a removable cover over the gel.109. The device of claim 106 , wherein said chamber comprises a round well.110. The device of claim 106 , wherein said one or more geometric features comprise posts.111. The device of claim 110 , wherein said posts comprise a concentric array of posts.112. The device of claim 106 , wherein said cells are cord blood cells.113. The device of claim 106 , further comprising endothelial cells in a second gel.114. The device of claim 113 , wherein said endothelial cells are human umbilical vein endothelial cells.115. A method of culturing cells claim 113 , comprising:1) providing a) microfluidic device comprising i) a chamber, said chamber comprising geometric features and a lumen, said lumen covered by ii) a removable lid and in fluidic communication with iii) one or more fluidic channels, b) ...

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26-01-2017 дата публикации

Horizontally Rocked BioReactor System

Номер: US20170022465A1
Автор: Ho Timothy Ray
Принадлежит:

Disclosed herein is an improved rocked bioreactor system used for carrying out cell and tissue cultures. 1. A bioreactor system comprising:a) one vessel assembly comprising at least one closed tubular rigid culture vessel; wherein said culture vessel comprises at least one tube and two closed ends made of single use or reusable material; wherein said culture vessel is positioned to lie along the longitudinal axis; wherein said culture vessel is configured to culture cells; wherein said culture vessel comprises at least one baffle; wherein the baffle contacts the inside surface and two closed ends of said culture vessel, wherein the baffle has a ratio of greater than 0.1 and less than 0.9 of heights of said baffle to the cross-sectional surface of said culture vessel perpendicular to the longitudinal axis; wherein said culture vessel comprises at least one media port configured to allow culture material to flow in and out of said culture vessel; and wherein said culture vessel comprises at least one set of two gas ports configured to allow the respiratory gas to flow in and out of said culture vessel;b) one reservoir assembly comprising at least one closed container made of single use or reusable material;c) one rocking apparatus configured to hold and position said culture vessel and rock about the longitudinal axis of the culture vessel along the plane perpendicular to said axis at angle of less than 360 degrees;d) one pumping apparatus fluidly coupled to at least one media port of said culture vessel and configured to pump culture material in and out of said culture vessel through said at least one port;e) one gassing apparatus coupled to at least one set of two gas ports of said culture vessel and configured to entering the gas through one gas port and out of said culture vessel through another gas port;f) one gas blending apparatus coupled to said gassing apparatus and configure to blend the gases including oxygen, nitrogen, carbon dioxide, or air;g) one ...

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10-02-2022 дата публикации

UNIVERSAL MICROFLUIDIC CULTURE SYSTEM TO ANALYZE AND CONTROL CELL DYNAMICS

Номер: US20220041966A1
Автор: TAY Savas, Zhang Ce
Принадлежит:

Microfluidic devices, systems, and methods. 1. A microfluidic device comprising: an inlet;', 'an outlet;', 'a recess defining a chamber with a first portion and a second portion, the first portion having a first depth in the z direction, and the second portion having a second depth in the z direction that is greater than the first depth, a first port in the first portion of the chamber;', 'a first channel extending between the inlet and the first port;', 'a first valve (Valve 1) in fluid communication with the first port and configured to be selectively opened to permit fluid communication between the first port and the first channel;', 'a second port in the first portion of the chamber;', 'a second valve (Valve 2) in fluid communication with the second port and configured to be selectively opened to permit fluid communication with the second port;', 'a third port in the first portion of the chamber;', 'a third valve (Valve 3) in fluid communication with the third port and configured to be selectively opened to permit fluid communication with the third port;', 'a fourth port in the second portion of the chamber;', 'a fourth valve (Valve 4) in fluid communication with the fourth port and configured to be selectively opened to permit fluid communication with the fourth port;', the first channel between inlet and the first valve,', 'the second valve such that the second channel fluidly communicates with the second port when the second valve is open,', 'the outlet at a point farther from the fourth port than is the fourth valve, and', 'the third valve such that the second channel fluidly communicates with the third port when the second valve is open;, 'a second channel sequentially connecting, 'a fifth valve (Valve 5) disposed in the second channel and configured to be selectively closed to prevent fluid communication between the second valve and the outlet;', the first channel at the first valve such that the third channel fluidly communicates with the first port when ...

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10-02-2022 дата публикации

CELL CULTURE CHIP

Номер: US20220041969A1
Принадлежит: USHIO DENKI KABUSHIKI KAISHA

The cell culture chip includes a bottom substrate and a base body part. The base body part includes a plurality of first portions for forming the culture space, and a cavity penetrating the base body part in a region where the first portions are not formed. The first portions each include a recessed region and a plurality of opening grooves that are formed through the base body part from a plurality of places inside the recessed region. In the cavity, at least a part of an end is positioned inside an outer edge of the base body part. The bottom substrate and the first surface of the base body part are bonded together to form the culture space in which the recessed region is sandwiched between the bottom substrate and the base body part. 1. A cell culture chip comprising a bottom substrate and a base body part bonded onto the bottom substrate , whereinthe base body part comprises:a first surface and a second surface opposing the first surface;a plurality of first portions configured to form a culture space and dispersedly arranged at a plurality of places in a direction parallel to the first surface; anda cavity that penetrates the base body part from the first surface and reaches the second surface, in a region where the first portions are not formed,the plurality of first portions each comprises:a recessed region formed to extend in the direction parallel to the first surface on a side of the first surface; anda plurality of opening grooves formed to penetrate the base body part from a plurality of places in the recessed region and reach the second surface,the cavity is configured such that at least a part of an end in the direction parallel to the first surface is positioned inside an outer edge of the base body part, andthe bottom substrate and the first surface of the base body part are bonded together to form the culture space in which the recessed region is sandwiched between the bottom substrate and the base body part.2. The cell culture chip according to ...

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28-01-2021 дата публикации

METHOD AND DEVICE FOR PREPARING AN IMPLANT OBTAINED FROM A CULTURE OF STEM CELLS

Номер: US20210023273A1
Принадлежит:

Disclosed is a method for preparing an implant including a culture of cells on a membrane, the method including steps that consist of: securing the membrane to a mounting; placing the membrane in a recess of a housing providing two spaces having adjusted heights, above and below the membrane; injecting, into the spaces, a liquid capable of transforming into gel at a transport temperature lower than an injection temperature of the liquid; and bringing the housing to the transport temperature, so as to form an implant including the membrane and two layers of gel having adjusted thicknesses, on two opposite faces of the membrane, respectively. 17073343016102038397172. A method of preparing an implant () comprising a culture of cells () on a membrane () , the method comprising the steps of: fixing the membrane on a support () , disposing the support in a housing recess () of a housing ( , ) leaving two spaces ( , ) having adjusted heights , above and below the membrane , injecting into the spaces a liquid capable of transforming into gel at a temperature of transport below an injection temperature of the liquid , and bring the housing to the transport temperature , so as to form an implant comprising the membrane and two layers of gel ( , ) having adjusted thicknesses , respectively on two opposite faces of the membrane.2703471723016. The method according to claim 1 , comprising a step of cutting the implant () in the membrane () and the two layers of gel ( claim 1 , ) claim 1 , the membrane being fixed to the support () and disposed in the housing recess ().31610506070347172. The method according to claim 1 , comprising a step of closing the housing recess () of the housing () receiving the membrane by an extraction cover () claim 1 , the extraction cover being configured to receive and guide a cutting tool () configured to cut the implant () in the membrane () and the two layers of gel ( claim 1 , ).470. The method according to claim 1 , wherein the implant () has an ...

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28-01-2021 дата публикации

MIKROFLUIDISCHER CHIP WITH AN UNVENTED GAS CAVITY IN A MICROFLUIDIC CHIP

Номер: US20210023553A1
Принадлежит:

An incubation segment in a microfluidic chip for microbiological assays, wherein said incubation segment is formed in a substrate with an upper major face and a lower major face, said segment includes an incubation well, an inlet channel through which a sample may be inputted into said incubation well, and a gas cavity connected to said incubation well by a microfluidic communication channel, wherein said gas cavity is unvented and the base of the gas cavity is connected to the base of the incubation well by the communication channel. 112-. (canceled)13. An incubation segment in a microfluidic chip for microbiological assays , wherein said incubation segment is formed in a substrate with an upper major face and a lower major face , said segment comprises an incubation well , an inlet channel through which a sample may be inputted into said incubation well , and a gas cavity connected to said incubation well by a microfluidic communication channel , wherein said gas cavity is unvented and that a base of the gas cavity is connected to a base of the incubation well by the communication channel.14. The incubation segment of claim 13 , wherein the volume of the incubation well Vis greater or equal to 0.5 μl and less than or equal to 5 μl claim 13 , the volume of the gas cavity Vg is greater or equal to 0.5 μl and less than or equal to 1.5 μl.15. The incubation segment of claim 14 , wherein the volume of the incubation well Vis greater or equal to 1 μl and less than or equal to 2.5 μl claim 14 , the volume of the gas cavity Vg is greater or equal to 0.7 μl and less than or equal to 1.3 μl.16. The incubation segment of claim 15 , wherein the volume of the incubation well Vis greater or equal to 2.2 μl and less than or equal to 2.4 μl claim 15 , the volume of the gas cavity Vis greater or equal to 0.9 μl and less than or equal to 1.2 μl.17. The incubation segment of claim 13 , wherein the volume of the gas cavity Vis greater than or equal to 5% of the volume Vof the ...

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23-01-2020 дата публикации

DEVICE FOR CELL CULTURE AND METHOD FOR CULTURING CELLS

Номер: US20200024561A1

Disclosed is a device for cell culture, the device includes a first chamber having an area for containing cells, such as isolated cells, for growth; a second chamber for containing culture medium; a semi permeable membrane forming at least part of a divider between the first chamber and the second chamber; wherein the area for containing cells for growth is adjustable via a moveable wall disposed within the first chamber. The device is especially suited for culturing of semi-adherent and non-adherent cells. 1. A device for cell culture , the device comprisinga first chamber having an area for containing cells for growth;a second chamber for containing culture medium;a semi-permeable membrane forming at least part of a divider between the first chamber and the second chamber;wherein the area for containing cells for growth is adjustable via a moveable wall disposed within the first chamber.2. The device of claim 1 , wherein the semi-permeable membrane comprises uniform micro-sized pores.3. The device of claim 2 , wherein the diameter of each micro-sized pore is between 0.1 to 2 micro-meters (μm).4. The device of claim 1 , wherein the semi-permeable membrane is supported or covered by a porous grid.5. The device of claim 1 , wherein the moveable wall is part of a piston and the first chamber comprises a port to receive the piston.6. The device of claim 5 , wherein the piston is operably movable along a longitudinal axis of the first chamber.7. The device of claim 6 , wherein the port is coated with a sealant.8. The device of claim 1 , wherein the moveable wall is coated with a sealant.9. The device of claim 7 , wherein the sealant is a bio-compatible rubber.10. The device of claim 1 , wherein the second chamber comprises at least one filtered opening shaped and dimensioned for gaseous exchange with the environment.11. The device of claim 1 , wherein the first chamber comprises at least one filtered opening shaped and dimensioned to receive cells.12. The device of ...

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23-01-2020 дата публикации

CELL TREATMENT APPARATUS

Номер: US20200024562A1
Принадлежит:

A cell treatment apparatus capable of treating cells in a cell culture vessel. The cell treatment apparatus () according to the present invention includes a first region (), a second region (), and a third region (). The first region () and the second region () are placed in succession. The first region () is a cell treatment chamber for treating cells. The cell treatment chamber can be closed from the outside of the cell treatment chamber and includes a culture vessel placement portion for placing a cell culture vessel. The second region () includes a laser irradiation device capable of irradiating the cell culture vessel placed in the culture vessel placement portion with a laser. The third region () includes a control device that controls at least one device in the cell treatment apparatus () and a power supply device () that supplies electric power to at least one device in the cell treatment apparatus (). The culture vessel placement portion is placed to be adjacent to the second region () in the cell treatment chamber. An adjacent portion to the second region () in the culture vessel placement portion is translucent. 1. A cell treatment apparatus comprising:a first region;a second region; anda third region, whereinthe first region and the second region are placed in succession,the first region is a cell treatment chamber for treating cells,the cell treatment chamber can be closed from the outside of the cell treatment chamber and comprises a culture vessel placement portion for placing a cell culture vessel,the second region comprises a laser irradiation device capable of irradiating the cell culture vessel placed in the culture vessel placement portion with a laser,the third region comprises a control device that controls at least one device in the cell treatment apparatus and a power supply device that supplies electric power to at least one device in the cell treatment apparatus,the culture vessel placement portion is placed to be adjacent to the second ...

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23-01-2020 дата публикации

HARDWARE FOR MAGNETIC 3D CULTURE

Номер: US20200024567A1
Автор: SOUZA Glauco
Принадлежит:

Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates, adjustable height cap for same, as well similar devices for multi-magnet culture plates. A pen-like device for sterilely lifting and moving cells is also described, and this magnetic pipettor can also exist in multi-well magnetic pipettor formats. 1. A magnetic pipettor for moving cells , comprising a magnet in a housing having a first end , and an overcap for fitting over said first end and protruding therefrom , and adjusting means for reversably lowering said magnet into said overcap.2. The magnetic pipettor of claim 1 , wherein said overcap is made of a non-adherent material and is sterilizable.3. The magnetic pipettor of claim 1 , wherein said adjusting means comprises detents and a spring over a rod coupled to said magnet to alternatively hold said magnet in an upper position inside said housing or a lower position inside said overcap.4. The magnetic pipettor of claim 1 , which comprises a single magnet claim 1 , single housing and single overcap.5. The magnetic pipettor of claim 1 , which comprises a plurality of magnets and a plurality of overcaps claim 1 , wherein adjusting means raises and lowers all of said plurality of magnets at the same time.6. The magnetic pipettor of claim 1 , further comprising a fiber optic cable for shining light through said overcap and wherein said overcap is optically transparent.7. The magnetic pipettor of claim 1 , wherein said overcap is cylindrically shaped with one closed end and one open end claim 1 , and wherein said open end fits over said first end of said housing.8. The magnetic pipettor of claim 7 , wherein said closed end has a concave depression therein.9. The magnetic pipettor of claim 8 , wherein said closed end is flared.10. The magnetic pipettor of claim 1 , wherein said overcap has a bend therein such a distal portion of said overcap is angled at least 30 degrees from said housing claim 1 , and said distal ...

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28-01-2021 дата публикации

CULTURE SYSTEMS AND METHODS OF USING SAME

Номер: US20210024861A1
Принадлежит: AUBURN UNIVERSITY

Culture systems and methods of using same. The systems include a housing defining an inner space. The inner space includes a headspace and at least a portion of a reservoir. A surface for immobilizing cells is moveable between the headspace and the reservoir. The systems can be used for coculturing methanotrophs and phototrophs for processing biogas and wastewater, particularly from anaerobic digesters. 1. A system comprising:a housing comprising a top and sides and defining an inner space extending between the sides and to the top;a headspace in an upper portion of the inner space;a reservoir comprising at least a first reservoir portion, wherein the first reservoir portion is in a lower portion of the inner space, the headspace and the reservoir are defined with respect to each other within the inner space by a horizontal plane spanning the inner space, and the headspace and the reservoir do not overlap within the inner space; anda surface comprising a surface portion capable of being cycled between the headspace and the reservoir.2. The system of claim 1 , wherein the housing separates the headspace from a surrounding space claim 1 , wherein the housing is capable of maintaining a gaseous composition of the headspace that is different from a gaseous composition of the surrounding space.3. The system of claim 1 , wherein the housing forms an enclosure enclosing the inner space claim 1 , wherein the reservoir is entirely encompassed within the inner space.4. The system of wherein the reservoir further includes a second reservoir portion contiguous with the first reservoir portion claim 1 , the second reservoir portion does not overlap with the first reservoir portion and does not overlap with the inner space claim 1 , the housing separates the headspace from a surrounding space claim 1 , and the second reservoir portion is contiguous with the surrounding space.5. The system of claim 1 , wherein the headspace is filled with a gas claim 1 , the gas comprises at least ...

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28-01-2021 дата публикации

SYSTEM AND METHOD FOR GENERATING AND TESTING TISSUE

Номер: US20210024862A1
Принадлежит:

Systems, devices, and methods for producing a tissue specimen and performing subsequent biomechanical testing thereof to quantify various biological/physiological properties and characteristics. The system may include a bioreactor device for producing the tissue specimen which includes a packaged/sealed seeding cup containing a scaffold in a sterile environment amenable to seeding with cells. After seeding, the cup may be stored in a standard laboratory culture tray for incubation. The produced tissue specimen may next be transferred to a testing instrument under control of a microcontroller which automatically performs a series of tests on the tissue and generates testing data communicated to an external electronic device which can operably interact with and control operation in part of the testing instrument via software. Tissue integrity in the instrument may be maintained by sterile fluid circulation and heating systems. The system advantageously provides efficient and reproducible tissue growth and analysis. 192-. (canceled)93. A packaged bioreactor device comprising:a cup including a bottom, a top, and an internal cavity extending between the bottom and the top;a lid sealing the top of the cup and being removable to access the cavity;a tissue scaffold supported in the cavity; anda mask removably disposed in the cavity above the tissue scaffold, the mask including a plurality of through holes to access the tissue scaffold and a portion of the cavity below the mask.94. The device according to claim 93 , further comprising a sterile fluid disposed in the cavity of the cup at least below the mask claim 93 , the tissue scaffold at least partially immersed in the fluid.95. The device according to claim 94 , wherein the fluid below the mask is accessible for aspiration through an aspiration hole of the through holes claim 94 , and which is laterally offset in a position on the mask from the tissue scaffold.96. The device according to claim 93 , wherein the through ...

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02-02-2017 дата публикации

METHOD AND APPARATUS FOR EXTRACTING CARBON DIOXIDE FROM AIR

Номер: US20170028347A1
Принадлежит:

A method and apparatus for extracting COfrom air comprising an anion exchange material formed in a matrix exposed to a flow of the air, and for delivering that extracted COto controlled environments. The present invention contemplates the extraction of CO from air using conventional extraction methods or by using one of the extraction methods disclosed; e.g., humidity swing or electro dialysis. The present invention also provides delivery of the COto greenhouses where increased levels of COwill improve conditions for growth. Alternatively, the COis fed to an algae culture. 17.-. (canceled)8. The apparatus of claim 47 , wherein the ion exchange resin comprises a heterogenous ion exchange membrane.9. (canceled)10. The apparatus of claim 48 , wherein the ion exchange resin comprises a strong base Type 1 or Type 2 functionality ion exchange resin.11. The apparatus of claim 47 , wherein the ion exchange resin is moveable from the outside to the inside of the greenhouse.12. The apparatus of claim 11 , wherein the ion exchange resin is mounted on a moveable fixture built into a wall of the greenhouse.13. The apparatus of claim 47 , wherein the ion exchange resin is included in an extractor that is located adjacent a lower end of a convection tower built adjacent the greenhouse.1420.-. (canceled)21. The apparatus of wherein the ion exchange resin comprises a first store of ion exchange resin; and the apparatus further comprises a second store of ion exchange resin claim 47 , and a selectively closed loop recirculation system for selectively recirculating air through the first store and the second store.22. The apparatus of claim 21 , including flow valves for controlling recirculation.23. (canceled)24. The apparatus of claim 21 , wherein the first store of ion exchange resin and the second store of ion exchange resin each comprise a strong base Type 1 or Type 2 functionality ion exchange resin.2546.-. (canceled)47. An apparatus for the capture of carbon dioxide from ambient ...

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24-04-2014 дата публикации

Cell chip container for storing cell chip

Номер: US20140113360A1
Автор: Bo Sung Ku, Dong Woo Lee
Принадлежит: Samsung Electro Mechanics Co Ltd

There is provided a cell chip including: a first substrate provided with a culture region receiving a bio-material and a humidity control space storing water for maintenance of humidity; a second substrate coupled to the first substrate; and a first wall member surrounding the culture region.

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29-01-2015 дата публикации

System For Generating Biogas And Method For Operating Such A System

Номер: US20150031104A1
Принадлежит:

A system for generating biogas comprises at least one fermenter () for fermenting solid biomass (). The fermenter () comprises a device for charging the biomass with a liquid medium, a gas outlet () for removing the biogas from the fermenter () and a gas inlet () for supplying gases to the fermenter (). The gas inlet () is connectable to a biogas source, preferably to the gas outlet (), a biogas line and/or a biogas reservoir. 111311321313231. Layout for the generating of biogas , which has at least one fermenter () for the fermenting of solid biomass () , wherein the fermenter () has a device for subjecting the biomass () to a liquid medium , a gas outlet () for taking away the biogas from the fermenter () and a gas inlet () for supplying gases to the fermenter () , wherein the gas inlet () can be connected to a biogas source , preferably to the gas outlet () , a biogas pipeline and/or a biogas reservoir , characterized in that the gas inlet () is designed such that the liquid medium after passing through the biomass is drained by means of it , and biogas is introduced into the fermenter during the supplying of the liquid medium to the fermenter ().23. Layout according to claim 1 , characterized in that the gas inlet () is configured as a gas distributing arrangement claim 1 , especially a spigot floor.3. Layout according to claim 1 , characterized in that{'b': 6', '3, 'the layout has a delivery arrangement () for delivering the biogas in the direction of the gas inlet ().'}423. Layout according to claim 1 , characterized in that the layout has a biogas reservoir claim 1 , which can be connected preferably to the gas outlet () and/or to the gas inlet ()51413. Layout according to claim 1 , characterized in that an arrangement () for lateral supporting of the biomass () is provided inside the fermenter claim 1 , being preferably permeable to liquids.631. Layout according to claim 1 , characterized in that the gas inlet () is arranged underneath the biomass being ...

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02-02-2017 дата публикации

CULTURE VENT PLUG, LID BODY KIT, AND FRAME BODY FOR CULTURE VENT PLUG

Номер: US20170029757A1
Принадлежит: TAIYO KOGYO CO., LTD.

A culture vent plug, a lid body kit, and a frame body for the culture vent plug are provided having a configuration which prevents air permeability from lowering when a vessel opening portion is blocked and excellent in cleanability and sterilizability. A vent plug body capable of securing ventilation between inside and outside a culture vessel; and a frame body including a holding portion holding the vent plug body, and an attachment portion capable of being attached to and detached from a vessel opening of the culture vessel are included, and the vent plug body is held by the holding portion so as to be capable of being repeatedly attached to and detached from the frame body. 1. A culture vent plug comprising:a vent plug body capable of securing ventilation between inside and outside a culture vessel; anda frame body including a holding portion holding the vent plug body, and an attachment portion capable of being attached to and detached from a vessel opening of the culture vessel, and an exposed end exposed from the vessel opening; anda lid body repeatedly and detachably attached to an exposed end portion of the frame body which includes the exposed end, and from which the vent plug body is exposed, whereinthe vent plug body is held by the holding portion so as to be capable of being repeatedly attached to and detached from the frame body, a wall portion provided in a peripheral direction, and', 'a ceiling portion extending from one end portion of the wall portion inward in a radial direction, the ceiling portion including a ceiling open hole from which the vent plug body is exposed,, 'the lid body includes'}each of the frame body and the lid body is configured of a rubber elastic body and the vent plug body is configured of a ceramic, anda diameter of the vent plug body is greater than an inner diameter of the holding portion, an outer diameter of the exposed end portion is greater than or equal to an inner diameter of the lid body, the vent plug body is press- ...

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17-02-2022 дата публикации

ARRANGEMENT FOR IMPLEMENTATION OF IN VITRO BIOCOMPATIBILITY TESTS

Номер: US20220048034A1

An assembly for performing in vitro biocompatibility tests, at least one sample is arranged on a surface of a base plate or the sample forms a surface or a surface region of the base plate. A holding element having at least one through-hole is placed onto the sample so that a first opening of the through-hole, which first opening is arranged facing the base plate, is arranged in the region of the sample. The through-hole, with the hollow space thereof, and the sample form a cavity. A cover element is placed onto and fastened on the holding element so that a compressive force acts on the holding element, which compressive force leads to at least partial deformation of the holding element and to a fluid-tight closure of the first opening of the through-hole, which first opening is arranged facing the base plate. 1. An arrangement for the implementation of in vitro biocompatibility tests , in which at least one sample is disposed on a surface of a base plate or in which the at least one sample forms a surface or a surface region of the base plate and a receiving element having at least one through-hole can be placed on the at least one sample such that a first opening , which is disposed pointing in the direction of the base plate , of the at least one through-hole is disposed in the region of the at least one sample ,the at least one through-hole with its hollow space and the sample forming a cavity, andthe receiving element being formed from or with an elastically deformable material;a cover element placed and fixed on the receiving element such that a pressure force acts on the receiving element, which leads to an at least partial deformation of the receiving element and the fluid-impermeable closure of the first opening, which is disposed pointing in the direction of the base plate, of the at least one through-hole,the base plate and the cover element being connected together on one side by at least one joint; the at least one joint and closing elements being ...

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17-02-2022 дата публикации

ADVANCED TISSUE ENGINEERING SYSTEM

Номер: US20220049199A1
Принадлежит:

The invention is an automated advanced tissue engineering system that comprises a housing in which one or more tissue engineering modules are accommodated together with a central microprocessor that controls functioning of the tissue engineering modules. In one embodiment, the tissue engineering module comprises a housing supporting one or more bioreactor chamber assemblies and a fluid reservoir operationally engageable with the housing. The bioreactor chamber assemblies may be selected depending on the end product option desired and may include, for example, a cell therapy bioreactor chamber, a single implant bioreactor chamber and a multiple (mosaic) implant bioreactor chamber. 1. A product chamber assembly , comprising:a bioreactor, the bioreactor having a bioreactor lid connected to a vertical chamber and a bioreactor base, the bioreactor containing a conical cell collector;a set of flexible bags that are used to contain processing fluids and for automated flow to and from the bioreactor, wherein at least one flexible bag is in fluid communication with the bioreactor; andat least one biosensor for monitoring parameters of the flexible bags and cells within the bioreactor, the at least one biosensor relaying variable parameter information to a microprocessor to process the parameters and dynamically adjust and adapt to specific needs of the cells.2. The product chamber assembly of claim 1 , further comprising a temperature controller to allow for reduced temperature operation.3. The product chamber assembly of claim 1 , further comprising a collection reservoir.4. The product chamber assembly of claim 1 , further comprising a fluid pump.5. The product chamber assembly of claim 1 , further comprising one or more fluid control valves. The present application is a continuation of U.S. application Ser. No. 17/013,100, filed Sep. 4, 2020, which is a continuation of U.S. application Ser. No. 15/293,611, filed Oct. 14, 2016, which is a divisional of U.S. application Ser ...

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17-02-2022 дата публикации

BIOPHARMACEUTICAL LIQUID RESERVOIR WITH MECHANICAL MEMBER INCLUDING ROTATING AND STATIONARY SETS OF PARTS

Номер: US20220049201A1
Принадлежит: SARTORIUS STEDIM FMT SAS

A biopharmaceutical liquid reservoir, includes a bag forming an inner storage space for storing a biopharmaceutical liquid, a mechanical member located at a wall of the bag, having: a stationary set of parts that is stationary with respect to the wall of the bag, a rotating set of parts rotating about an axis of rotation with respect to the stationary set, a bearing located between the two sets and, inside the bag, a communication passage: separating the bearing from the inner storage space, including one or more changes of direction, being formed by a portion of the parts of the rotating set located opposite a portion of the parts of the stationary set. 1. A biopharmaceutical liquid vessel , comprising:a bag forming an inner storage space for storing biopharmaceutical liquid,a mechanical member located at a wall of the bag, comprising:a set of stationary parts, stationary with respect to the wall of the bag,a set of rotating parts, rotating about an axis of rotation with respect to the set of stationary parts,a bearing located between the set of stationary parts and the set of rotating parts and in an inner storage space inside the bag,a communication passage separating the bearing of the inner storage space, comprising one or more changes of direction being formed by a portion of the parts of the set of rotating parts located opposite a portion of the parts of the set of stationary parts.2. The biopharmaceutical liquid vessel according to claim 1 , wherein the passage forms an inverted siphon claim 1 , of the air being trapped in a bottom of the inverted siphon by the liquid located at least in one branch of the inverted siphon starting at the bottom of the inverted siphon and opening into the inner storage space.3. The biopharmaceutical liquid vessel according to claim 2 , wherein said air being trapped in the bottom of the inverted siphon by the liquid located at least in two branches of the at least one branch of the inverted siphon claim 2 , a first branch of ...

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17-02-2022 дата публикации

HIGHLY EFFICIENT GAS PERMEABLE DEVICES AND METHODS FOR CULTURING CELLS

Номер: US20220049215A1
Автор: Wilson John R.
Принадлежит: Wilson Wolf Manufacturing Corporation

This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced. 120.-. (canceled)21. A static cell growth apparatus , comprising:{'claim-text': ['said apparatus does not include equipment to pump gas or medium through the apparatus, and wherein', 'each gas permeable shelf has an opposing surface, the space between each gas permeable shelf and its opposing surface being a cell culture compartment, wherein each cell culture compartment has the same volume of space and the space is capable of being filled with medium;', 'an outside surface of each gas permeable shelf is in contact with projections that are spaced apart and are configured to create a space for incubator gas to contact the outside surface of each gas permeable shelf and to maintain each gas permeable shelf in a horizontal position during cell growth; and', 'the culture compartments reside one above the other when the shelves are in a horizontal position; and', 'a manifold connecting each culture compartment.'], '#text': 'a housing comprised of one or more walls, where all of the one or more walls are liquid impermeable and ...

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31-01-2019 дата публикации

SINGLE-USE CONNECTION DEVICE

Номер: US20190031995A1
Принадлежит: Eppendorf AG

The invention relates to a single-use connection device for insertion into a connection opening of a bioreactor, in particular in a connection opening of a top plate of a dimensionally-stable bioreactor or in a connection opening of a connection element of a pouch bioreactor. The single-use connection device is inserted in a connection opening of a top plate of a bioreactor and/or in a connection opening of a pouch reactor and is provided with multiple passages and an attachment section, wherein the single-use connection device is formed as a single piece and is made of plastic. The attachment section, on an outer peripheral surface, has an attachment structure for attaching the single-use connection device in the connection opening. 115.-. (canceled)16. A single-use connection device for insertion into a connection opening of a bioreactor , the single-use connection device comprising:a plurality of passages through the single-use connection device; anda fastening section comprising an outer circumferential surface having a fastening structure operably coupling the single-use connection device within the connection opening;wherein the single-use connection device is formed in one piece and from plastic.17. The single-use connection device of claim 16 , wherein the connection opening of the bioreactor comprises a connection opening of a top plate of a dimensionally stable bioreactor or a connection opening of a pouch bioreactor.18. The single-use connection device of claim 16 , wherein the fastening structure further comprises an external thread claim 16 , ribs claim 16 , projections claim 16 , snap-action elements claim 16 , detent elements claim 16 , or depressions.19. The single-use connection device of claim 16 , wherein the fastening section further comprises an encircling stop surface that extends outward in a radial direction from an outer circumferential surface.20. The single-use connection device of claim 19 , wherein the fastening section further comprises ...

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31-01-2019 дата публикации

CULTURE PEEL PLATE

Номер: US20190032001A1
Принадлежит: Charm Sciences,Inc.

Culture medium devices and systems are shown and described. In one embodiment, a peel plate includes a recessed well, a raised platform, and a removable adhesive cover. In certain examples, the recessed well receives a sample on a dried media culture disc. The removable cover may have a first end secured adjacent to the raised platform and a second removable end removably enclosing the recessed well. The result is a peel plate, assembly, and methods for the enumerating a microorganism, when present, in a sample. 1. A peel plate for enumerating a microorganism , when present , in a sample , said peel plate comprising:a. a recessed well having a sunken wall protruding from an upper face;b. a pair of opposing proximate extensions adjacent said recessed well, wherein at least one of said proximate extensions includes a raised proximate tab adapted to align multiple plates in a layered positioned; andc. a distal raised platform adjacent said recessed well, and wherein said recessed well being spaced between said distal raised platform and said proximate extensions.2. The plate of claim 1 , further including a removable cover seal adapted to removably enclose said recessed well.3. The plate of claim 2 , wherein said cover seal includes a peel tab removably secured to a proximate end of said plate.4. The plate of claim 1 , wherein said recessed well being aligned below and parallel to an upper face of said plate and includes a grid.5. The plate of claim 4 , wherein said grid being visible for colony counting after said sample has absorbed and diffused.6. The plate of claim 1 , wherein said proximate extensions being spaced between an access indent.7. The plate of claim 6 , wherein said proximate extensions each include a rounded corner entry to said access indent.8. The plate of claim 1 , wherein said distal raised platform spans about a length of a diameter of said recessed well.9. The plate of claim 1 , further including a culture medium secured in said recessed well.10. ...

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31-01-2019 дата публикации

Organ Chips And Uses Thereof

Номер: US20190032021A1
Принадлежит:

Disclosed herein are organ chips that can be individually used or integrated together to form different microphysiological systems, e.g., for use in cell culturing, drug screening, toxicity assays, personalized therapeutic treatment, scaffolding in tissue repair and/or replacement, and/or pharmacokinetic or pharmacodynamics studies. 1. An in vitro microphysiological system comprising: (i) a first organ chip comprising: a body comprising a central channel therein, and an at least partially porous and at least partially flexible first membrane positioned within the central channel and along a plane, wherein the first membrane is configured to separate the central channel to form two sub-channels, wherein one side of the first membrane is seeded with vascular endothelial cells, and the other side of the first membrane is seeded with at least one type of organ-specific parenchymal cells;', '(ii) a second organ chip comprising: a body comprising a first chamber enclosing a plurality of muscular thin films adapted to measure contraction of muscle cells, and a second chamber comprising a layer of muscle cells on the bottom surface of the second chamber, wherein the bottom surface is embedded with an array of microelectrodes for recording of action potentials, and wherein the top surface of the second chamber is placed with at least a pair of electrodes for providing electric field stimulation to the muscle cells; or', '(iii) a combination of the first organ chip and the second organ chip; and, 'a. at least two different organ chips, wherein said at least two different organ chips are selected from either one or both of the followingb. at least one connecting means between said at least two different organ chips.240-. (canceled)41. The system of claim 1 , wherein the system comprises at least three organ chips.42. The system of claim 1 , wherein the connecting means comprises a tubing that fluidically connects an outlet of one of the organ chips to an inlet of another organ ...

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30-01-2020 дата публикации

CELL SAMPLE LIQUID FEEDING BAG, CELL SAMPLE LIQUID FEEDING METHOD, AND CELL SAMPLE LIQUID FEEDING DEVICE

Номер: US20200032184A1
Принадлежит:

The present technology provides a technology capable of performing stable liquid feeding. For this, the present technology provides a cell sample liquid feeding bag or the like including at least: an outflow port from which cell sample liquid flows out; a bottom portion including a reservoir unit capable of reserving cells and at least partly including a slope; and a first inner tube extending from the outflow port toward the reservoir unit to a position not contacting the reservoir unit, and the cell sample liquid is fed from a reservoir unit side toward an outflow port side of the first inner tube. 1. A cell sample liquid feeding bag comprising at least:an outflow port from which cell sample liquid flows out;a bottom portion including a reservoir unit capable of reserving cells and at least partly including a slope; anda first inner tube extending from the outflow port toward the reservoir unit up to a position not contacting the reservoir unit,wherein the cell sample liquid is fed from a reservoir unit side toward an outflow port side of the first inner tube.2. The cell sample liquid feeding bag according to claim 1 , wherein a cross-sectional shape of the bottom portion has a substantially V-shape.3. The cell sample liquid feeding bag according to claim 1 , further comprising an outer tube extending from the outflow port toward outside of the bag claim 1 ,wherein the outer tube includes at least two or more branched paths.4. The cell sample liquid feeding bag according to claim 1 , further comprising at least one or more ports besides the outflow port.5. The cell sample liquid feeding bag according to claim 4 , wherein the port/ports is/are provided at a bag wall surface.6. The cell sample liquid feeding bag according to claim 5 , whereinone of the ports further includes a second inner tube extending toward inside of the bag, andthe second inner tube is used for cell sample liquid injection.7. The cell sample liquid feeding bag according to claim 6 , wherein an ...

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30-01-2020 дата публикации

FLUIDIC CHIP FOR CELL CULTURE USE, CULTURE VESSEL, AND CULTURE METHOD

Номер: US20200032186A1
Автор: HAGIWARA Masaya
Принадлежит:

The present invention provides a fluidic chip for cell culture use which can prevent a decrease in the activity of cultured cells in a preparation stage, and which makes it possible to observe a cultured cell tissue while detaching the cultured cell tissue from the fluidic chip. The fluidic chip according to the present invention is characterized by being provided with a base and a lid member, wherein the base and the lid member are configured in such a manner that a first flow path and a first accommodation section for attachably/detachably accommodating a first culture vessel are formed between the base and the lid member when the base and the lid member are bonded to each other, the first culture vessel encloses a culture gel into which cells or a cell tissue is to be embedded and is at least partially formed from a hydrogel or a porous body, and the base and the lid member are configured in such a manner that at least a portion of a culture solution flowing through the first flow path is supplied to the first accommodation section. 1. A fluidic chip for cell culture use , comprising a base and a lid member , whereinthe base and the lid member are configured in such a manner that a first flow path and a first accommodation section for attachably/detachably accommodating a first culture vessel are formed between the base and the lid member when the base and the lid member are bonded to each other,the first culture vessel encloses a culture gel into which cells or a cell tissue is to be embedded and is at least partially formed from a hydrogel or a porous body,the first accommodation section has a socket with which the first culture vessel is engageable, andthe base and the lid member are configured in such a manner that at least a portion of a culture solution flowing through the first flow path is supplied to the first accommodation section.2. The fluidic chip according to claim 1 , whereinthe base or the lid member has a first inlet and a first outlet, andthe ...

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30-01-2020 дата публикации

WELL PLATE INCUBATOR

Номер: US20200032193A1
Принадлежит:

Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided. 1. An incubator comprising:an enclosure having an internal chamber configured to support a cell culture plate comprising a plurality of wells, wherein the enclosure comprises at least one passage configured for gas entry, a connector adapted to connect a pressurized gas source to the at least one passage, and a plurality of openings configured to allow access to the plurality of wells of the cell culture plate;a controller configured to maintain a temperature of the internal chamber within a desired range;a first heating/cooling device engaged directly or indirectly with the enclosure, the first heating/cooling device controlled by the controller; anda sealing element comprising a first plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure,wherein the sealing element is configured to form a seal with the plurality of openings in the enclosure that allows the enclosure to maintain a pressure in the internal chamber between about 0.0005 psi to about 0.01000 psi above ambient pressure when gas from the pressurized gas source flows through the connector and at least one passage into the internal chamber,wherein the sealing element is movable between a closed position where the sealing element occludes, and thereby seals, each of the plurality of ...

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04-02-2021 дата публикации

ANAEROBIC PHOTOBIOREACTOR AND METHOD FOR BIOMASS CULTIVATION, WASTEWATER TREATMENT, NUTRIENTS RECOVERY, ENERGY PRODUCTION AND HIGH-VALUE PRODUCTS SYNTHESIS

Номер: US20210032581A1
Принадлежит:

The present invention is related to an anaerobic photobioreactor and a method for active biomass cultivation, wastewater treatment, nutrients recovery, energy production and high-value products synthesis. Phototrophic bacteria are cultured in the anaerobic photobioreactor lighted with solar or artificial irradiation where certain light wavelengths are selectively discarded with a light selector installed on the top of the photobioreactor. In this light-based process wastewater treatment and resources recovery, like nutrients and high-value bioproducts (fertilizers, polymers and proteins) present in wastewater are performed simultaneously. Cultured biomass is treated by anaerobic digestion for biofuel production, including optative hydrolytic pre-treatment, and/or valuable bioproducts can be obtained in a downstream process. 1. An anaerobic photobioreactor for biomass cultivation , wastewater treatment , nutrients recovery , energy production and high-value products synthesis , comprising:a. a horizontal closed anaerobic ditch;b. an inlet and an outlet of wastewater, both connected to an internal space of the anaerobic ditch body;c. a covered wastewater circulation system inside the anaerobic ditch;d. a lid covering the anaerobic ditch to block light at wavelengths below 750 nm;e. a cylindrical sampling tube submerged into the anaerobic ditch and stuck to the lid, to perform water and biomass analysis and control the headspace pressure;f. a biomass recirculation pipe from a biomass to the anaerobic ditch; andg. a biomass outlet line from the biomass separator.2. The anaerobic photobioreactor according to claim 1 , wherein the photobiorector is lightened with a light source characterized by a wavelength between 750 and 1200 nm.3. The anaerobic photobioreactor according to claim 2 , wherein the light is solar irradiation.4. The anaerobic photobioreactor according to claim 1 , wherein the covering lids are made of glass or PPMA claim 1 , and the selective monochromatic ...

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04-02-2021 дата публикации

ADVANCED TISSUE ENGINEERING SYSTEM

Номер: US20210032583A1
Принадлежит:

The invention is an automated advanced tissue engineering system that comprises a housing in which one or more tissue engineering modules are accommodated together with a central microprocessor that controls functioning of the tissue engineering modules. In one embodiment, the tissue engineering module comprises a housing supporting one or more bioreactor chamber assemblies and a fluid reservoir operationally engageable with the housing. The bioreactor chamber assemblies may be selected depending on the end product option desired and may include, for example, a cell therapy bioreactor chamber, a single implant bioreactor chamber and a multiple (mosaic) implant bioreactor chamber. 1. A product chamber assembly , comprising:a bioreactor;a set of flexible bags that are used to contain processing fluids and for automated flow to and from the bioreactor, wherein at least one flexible bag operates at reduced temperature from the bioreactor, and wherein at least one flexible bag is operationally engaged with a collection reservoir; andat least one biosensor for the monitoring of parameters of the flexible bags and cells within the bioreactor, the at least one biosensor relaying variable parameter information to a microprocessor to process the parameters and dynamically adjust and adapt to specific needs of cells during cell proliferation.2. The product chamber assembly of claim 1 , wherein the set of flexible bags and the bioreactor are connected via at least one of a passageway claim 1 , tubing claim 1 , connector claim 1 , valve claim 1 , pump claim 1 , filter claim 1 , fluid access port claim 1 , in-line gas exchange membrane claim 1 , and in-line sensor.3. The product chamber assembly of claim 1 , wherein the product chamber assembly provides an environment for at least one of the following: cell sorting claim 1 , cell washing claim 1 , cell concentrating claim 1 , cell seeding claim 1 , cell proliferation claim 1 , cell storage claim 1 , and cell transport.4. The ...

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01-05-2014 дата публикации

Multi-Layered Cell Culture Vessel

Номер: US20140120607A1
Автор: Abraham Elizabeth J.
Принадлежит: CORNING INCORPORATED

A multi-layered cell culture vessel is provided herein which includes a body enclosing a closed volume. The body includes at least one insert layer located within the closed volume to separate portions thereof, and at least one port allowing communication with the closed volume from exteriorly of the body. At least one grip may be provided which protrudes from the body. Additional features may include the use of a side port which is disposed transversely to a longitudinal axis of the body, including being disposed to intersect a gap located adjacent to each of the insert layers. This arrangement allows for the preparation and removal of cell culture medium without obstruction from the insert layers. Additionally, the end of the body may be formed with tapered portions that channel flow towards an end port thereby allowing for a relatively well-defined stream to be poured from the device as needed. 1. A multi-layered cell culture vessel comprising:a body enclosing a closed volume, said body including at least one insert layer located within said closed volume to separate portions thereof, and at least one port allowing communication with said closed volume from exteriorly of said body; and,at least one grip protruding from said body configured to be grippingly engaged by a user in transporting the multi-layered cell culture vessel.2. A cell culture vessel as in claim 1 , wherein said grip is arcuately formed with ends fixed to said body and a space being defined between said grip and body configured to receive a hand of a user.3. A cell culture vessel as in claim 1 , wherein said at least one port includes a side port protruding from said body in a direction generally parallel to said grip claim 1 , said grip protruding no further from said body than said side port with a cap mounted thereto.4. A cell culture vessel as in claim 1 , wherein said grip is spaced from said at least one port.5. A cell culture vessel as in claim 1 , wherein said body including first and ...

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09-02-2017 дата публикации

Biologically-relevant selective enclosures for promoting growth and vascularization

Номер: US20170037356A1
Принадлежит: Lockheed Martin Corp

Two-dimensional materials, particularly graphene-based materials, having a plurality of apertures therein can be formed into enclosures for various substances and a fibrous layer can be provided on an outside and/or on an inside of the enclosure. The enclosures can be exposed to an environment, such as a biological environment (in vivo or in vitro), where the fibrous layer can promote vascular ingrowth. One or more substances within the enclosure can be released into the environment, one or more selected substances from the environment can enter the enclosure, one or more selected substances from the environment can be prevented from entering the enclosure, one or more selected substances can be retained within the enclosure, or combinations thereof. The enclosure can, for example, allow a sense-response paradigm to be realized. The enclosure can, for example, provide immunoisolation for materials, such as living cells, retained therein.

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08-02-2018 дата публикации

Nanofluidic Device for Isolating, Growing, and Characterizing Microbial Cells

Номер: US20180037924A1
Автор: Epstein Slava
Принадлежит:

Nanofluidic devices and methods of the invention are capable of autonomously isolating individual microbial cells using constrictive channels and growing monocultures of the cells for automated characterization of their biochemical properties and interactions with mammalian cells. Single microbial cells, such as bacterial cells, are isolated directly from environmental sources and cultured using chemical factors from their native environment. 1. A method of isolating and culturing a single microbial cell to obtain a monoculture of microbial cells , the method comprising the steps of:(a) exposing a fluid sample containing a mixture of microbial cells to a device comprising a food chamber in fluid communication with an opening in the device, wherein the food chamber contains a culture medium containing a chemoattractant for at least one microbial cell in the sample and capable of supporting the growth and reproduction of said microbial cell, and wherein the opening allows only partial entry of a single microbial cell;(b) allowing said microbial cell to migrate into the opening of the device;(c) maintaining the device under conditions suitable for allowing said microbial cell to divide while residing in the opening and produce progeny, whereby the progeny eventually enter the food chamber;(d) maintaining the device under conditions suitable for the progeny entering the food chamber to multiply in the food chamber, forming a monoculture of microbial cells.2. The method of claim 1 , wherein the device comprises a plurality of microfluidic food chambers claim 1 , each fluidically coupled to a separate opening in the device; and wherein a plurality of monocultures are obtained claim 1 , each grown in a distinct food chamber and derived from a distinct single microbial cell of the sample.3. The method of claim 2 , wherein the device comprises 100 or more food chambers claim 2 , each fluidically coupled to a separate opening in the device.4. The method of claim 2 , whereby ...

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07-02-2019 дата публикации

DEVICE AND METHOD FOR SAMPLE ANALYSIS

Номер: US20190040344A1
Принадлежит:

The present invention describes a device used to process a sample and detect the presence or absence of certain molecules in the sample. The device includes a pouch having two or more compartments that may be created by sealing two or more films. In a preferred embodiment, the pouch is made by a single sheet of film that has been folded onto itself. In another preferred embodiment, the pouch is made by sealing two films. In other aspects, this invention describes methods to process a sample. 2. The pouch of claim 1 , wherein the pouch is made by heat-sealing one or two films.3. The pouch of claim 1 , wherein at least one of the flow channels has a substantially U-shape claim 1 , and wherein the single device comprises a clamp that exerts pressure on at least two surfaces of the flow channel.4. The pouch of claim 1 , wherein the pouch comprises at least five compartments and at least five flow channels claim 1 , and wherein the single device is associated with the pouch and is capable of closing the at least five flow channels at the same time.6. The pouch of wherein the first and second fluidic connectors are releasable fluidic connectors.7. The pouch of wherein the first and second fluidic connectors are hollow needles.8. The pouch of wherein the first and second fluidic connectors are Luer taper connectors.9. The pouch of claim 5 , wherein the pouch is made by heat-sealing one or two films.10. The pouch of claim 7 , wherein the needles are substantially parallel to each other.11. The pouch of claim 7 , wherein the needles are positioned to be from about 1 to about 30 millimeters apart.12. The pouch of claim 5 , wherein the first and second fluid connections are configured such that they are capable of being closed with a single device that exerts pressure on such fluid connections.13. The pouch of claim 12 , wherein the first and second fluid connections comprise substantially U-shaped channels.15. The pouch of claim 14 , wherein the container comprises a cap that ...

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07-02-2019 дата публикации

PREPARED PLATED MEDIA PRODUCT

Номер: US20190040346A1
Автор: Crandall Ryan D.
Принадлежит: BECTON DICKINSON AND COMPANY

The present invention relates to systems and methods involving interconnected plate components, including bases, lids and covers, interconnected in a manner such that a continuous strip of each component is prepared. The continuous strips may be stored as rollstock in a reel style. The physical properties of each continuous strip allow the base, lid and/or cover to include means for positive control. In one embodiment, the continuous strip of bases is advanced and processed through an automated system with positive control, and remains in the form of a continuous strip until agar in the bases is cured, at which time the bases are singulated. When a lid is applied to a base using methods described herein, an airtight seal is formed improving the quality of culture media used in testing. 1. A system of dispensable plates for preparing and inoculating media comprising:a first continuous strip of bases having a reservoir portion and a lip surrounding the reservoir;a second continuous strip of lids, each lid adapted to be placed on and attached to the lip of the base;a third continuous strip of covers having a peripheral planar surface extending between an at least one edge of the cover and a raised portion remote from the at least one edge of the cover, the raised portion rising from the peripheral planar surface such that the raised portion includes an at least one wall extending upward from the peripheral planar surface, each cover adapted to be placed over the lip of the base and attached directly to the base.2. The system of claim 1 , wherein the material of the base claim 1 , the lid and the cover is a thermoplastic.3. The system of claim 1 , wherein the base claim 1 , the lid and the cover are adapted to advance through an automated injection and inoculation system subject to positive control.4. The system of claim 3 , wherein the base claim 3 , the lid and the cover include a plurality of timing features.5. The system of claim 1 , wherein the at least one wall of ...

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07-02-2019 дата публикации

CONTROLLING PRESSURE

Номер: US20190040348A1
Принадлежит:

A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. 1. A method of controlling pressure while perfusing cells , comprising: A) providing a) a plurality of microfluidic devices , each of said microfluidic devices comprising i) one or more microchannels comprising living cells and ii) one or more reservoirs comprising culture media , b) one or more pressure actuators , B) coupling said pressure actuators to at least one of the said reservoirs , the coupling adapted such that actuated pressure modulates the perfusion of at least some of said living cells , C) turning said one or more pressure actuators between two or more pressure setpoints , thereby controlling pressure while perfusing said cells.2. The method of claim 1 , wherein said pressure actuators are turned between two or more setpoints in a switching pattern.3. The method of claim 2 , wherein the switching pattern is selected such that the average value of pressure acting liquid in said one or more reservoirs corresponds to a desired value.4. The method of claim 3 , wherein the switching pattern is selected such that the average gas pressure is maintained below 1 kPa.5. The method of claim 1 , wherein each of the microfluidic device comprises a top channel claim 1 , a bottom channel claim 1 , and a ...

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07-02-2019 дата публикации

CONTROLLING PRESSURE

Номер: US20190040349A1
Принадлежит:

A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. 1. (canceled)2. (canceled)3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. (canceled)9. (canceled)10. (canceled)11. (canceled)12. (canceled)13. (canceled)14. (canceled)15. A method of controlling pressure while perfusing cells , comprising: A) providing a) a culture module , said culture module comprising i) an actuation assembly configured to move ii) a pressure manifold , said pressure manifold comprising a mating surface with pressure points , and iii) one or more pressure controllers to provide pressure to said pressure points; and b) a plurality of microfluidic devices , each of said microfluidic devices comprising i) one or more microchannels comprising living cells , ii) one or more reservoirs comprising culture media , and iii) a cover assembly above said one or more reservoirs , said cover assembly comprising a cover with ports that correspond to the pressure points on the pressure manifold mating surface; B) placing said plurality of microfluidic devices on or in said culture module; C) contacting said ports on the cover of each microfluidic device of said plurality of microfluidic devices with said mating surface of said pressure manifold , such that the ports are in contact with ...

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06-02-2020 дата публикации

MICROBIOREACTOR MODULE

Номер: US20200040292A1
Автор: ROTH Yoen Ok
Принадлежит:

The invention relates to a microbioreactor module for the three-dimensional cultivation of cells, especially stem cells. Said microbioreactor is intended for single use and, in an embodiment of the invention, can be used as a multi-microbioreactor. 1. A microbioreactor module comprising a cultivation container , a mounting tube , an asymmetrical plug , and a sheathing , the microbioreactor module being characterized in thatthe cultivation container has an outer shell of a semi-permeable membrane material enclosing a biocompatible carrier material or scaffold,the mounting tube is attached to the cultivation container and encloses a discharge and supply line which allows samples to be taken and cells or bioactive molecules and nutrients to be supplied to the cultivation container,the asymmetrical plug has an asymmetrical length, projects into the interior of the microbioreactor module, fixes the mounting tube in an opening, and seals the microbioreactor module to the outside by contact with the sheathing, and opens or closes openings in the wall of the sheathing depending on the positioning.2. The microbioreactor module according to claim 1 , characterized in that the outer shell of the cultivation container comprises a bag of a semi-permeable organic or inorganic membrane material with an exclusion size of 1-50 kDa claim 1 , for example a dialysis hose material.3. The microbioreactor module according to claim 1 , characterized in that the carrier material in the cultivation container is comprised of natural or synthetic polymers or inorganic materials or a combination thereof claim 1 , or a corresponding scaffold.48. The microbioreactor module according to claim 1 , characterized in that the mounting tube claim 1 , the discharge and supply line and the sheathing comprises an elastic claim 1 , gas-permeable material claim 1 , and that the sheathing comprises a plurality of through-flow openings () which can be closed by flaps.5. The microbioreactor module according to ...

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06-02-2020 дата публикации

Device for evaluation of chemical substance and method for evaluation of chemical substance

Номер: US20200040294A1

There are provided a device and a method for more appropriate evaluation of a chemical substance. The device for the evaluation of the chemical substance includes a first compartment, and a second compartment that communicates with the first compartment and is separated from the first compartment by a partition wall.

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16-02-2017 дата публикации

Methods of anaerobic digestion of biomass to produce biogas

Номер: US20170044475A1
Принадлежит: Novus Energy LLC

Improved methods for anaerobic digestion of organic matter to produce biogas. Among the improvements given are including ferric iron in a hydrolysis reactor to increase the rate and efficiency of anaerobic hydrolysis to provide substrates for methanogenesis. A solids separation step is added after hydrolysis and before methanogenesis to improve the efficiency of the methanogenesis step. Other improvements involve using separate tanks for the hydrolysis and methanogenesis stages and using two (or more) methanogenesis tanks in sequence, and switching the order of the two (or more) methanogenesis tanks periodically.

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16-02-2017 дата публикации

BAG ASSEMBLY FOR CULTIVATION OF CELLS

Номер: US20170044477A1
Принадлежит:

The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly. 1. A flexible bag assembly for cultivation of cells , comprising one or more bags forming a plurality of cultivation compartments , wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means.2. The flexible bag assembly of claim 1 , wherein said second cultivation compartment has a volume of at least 120% claim 1 , such as at least 150% of the volume of the first cultivation compartment.3. The flexible bag assembly of claim 1 , comprising at least three cultivation compartments and wherein a drain port in the second cultivation compartment is adapted to be fluidically connected with a third cultivation compartment upon opening of a valve means.4. The flexible bag assembly of claim 3 , wherein said third cultivation compartment has a volume of at least 120% claim 3 , such as at least 150% of the volume of the second cultivation compartment.5. The flexible bag assembly of claim 1 , wherein at least one cultivation compartment claim 1 , such as the first cultivation compartment claim 1 , is a rigid or semirigid vessel.6. The flexible bag assembly of claim 1 , wherein said valve means is a valve or a clamp.7. The flexible bag assembly of claim 6 , wherein said valve means is a pinch valve or a pinch clamp.8. The flexible bag assembly of claim 7 , wherein said pinch valve or pinch clamp is adapted for application on tubing or conduits of up to 15 mm outer diameter.9. The flexible bag assembly of any preceding claim ...

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16-02-2017 дата публикации

Dispase-based junctional protein tensor dish

Номер: US20170044479A1
Принадлежит: NATIONAL UNIVERSITY OF SINGAPORE

Cell-culture devices and methods for determining the integrity of cell-cell adhesion are described. A cell culture device includes a channel-defining body that defines a plurality of channels in a cell-culture dish and a removable mask located above the channel-defining body. Channels of the cell-culture dish can be seeded and maintained under conditions in which a cell sheet can be formed in each of the channels. The mask defines test regions of cell sheets when cell sheets are present within the channels. A cell-substrate cleaving solution can be applied to the test regions to lift the cell sheet in the test region, causing the inherent tension due to cell spreading to be borne solely by the cell-cell junctions, such that the integrity of the cell sheets can be observed. The tension in the lifted cell sheet can be controlled by controlling the vertical design width of the channel on either side of the constriction included in the test region.

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16-02-2017 дата публикации

METHODS OF ANAEROBIC DIGESTION OF BIOMASS TO PRODUCE BIOGAS

Номер: US20170044574A1
Принадлежит:

Improved methods for anaerobic digestion of organic matter to produce biogas. Among the improvements given are including ferric iron in a hydrolysis reactor to increase the rate and efficiency of anaerobic hydrolysis to provide substrates for methanogenesis. A solids separation step is added after hydrolysis and before methanogenesis to improve the efficiency of the methanogenesis step. Other improvements involve using separate tanks for the hydrolysis and methanogenesis stages and using two (or more) methanogenesis tanks in sequence, and switching the order of the two (or more) methanogenesis tanks periodically. 123-. (canceled)24. A method of producing biogas comprising:(a) hydrolyzing an organic substrate in anaerobic condition in a hydrolysis tank to produce a hydrolysis liquid effluent;{'b': 1', '1, '(b) transferring the hydrolysis liquid effluent to a methanogenesis tank comprising fixed film methanogens or granular methanogens and producing biogas and methanogenesis tank liquid effluent;'}{'b': '1', 'wherein the methanogenesis tank has a liquid substrate and a recirculation loop for recirculating liquid substrate in the methanogenesis tank, the recirculation loop comprising a heating element in contact with liquid substrate in the recirculation loop;'}(c) recirculating the liquid substrate through the recirculation loop and returning the liquid substrate to remainder of the methanogenesis tank; and(d) heating the liquid substrate in the recirculation loop with the heating element;wherein the liquid substrate in the recirculation loop is depleted in the fixed film methanogens or the granular methanogens compared to liquid substrate in the remainder of the methanogenesis tank.25. The method of wherein the methanogenesis tank comprises granular methanogens fixed film methanogens and the liquid substrate in the recirculation loop is depleted in the granular methanogens compared to the liquid substrate in the remainder of the methanogenesis tank.26. The method of ...

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16-02-2017 дата публикации

METHODS OF ANAEROBIC DIGESTION OF BIOMASS TO PRODUCE BIOGAS

Номер: US20170044575A1
Принадлежит:

Improved methods for anaerobic digestion of organic matter to produce biogas. Among the improvements given are including ferric iron in a hydrolysis reactor to increase the rate and efficiency of anaerobic hydrolysis to provide substrates for methanogenesis. A solids separation step is added after hydrolysis and before methanogenesis to improve the efficiency of the methanogenesis step. Other improvements involve using separate tanks for the hydrolysis and methanogenesis stages and using two (or more) methanogenesis tanks in sequence, and switching the order of the two (or more) methanogenesis tanks periodically. 15. A method of producing biogas comprising:(a)(ii) transferring an organic substrate to a hydrolysis tank;(a)(iii) hydrolyzing the substrate in anaerobic condition in a hydrolysis tank to produce a hydrolysis liquid effluent;{'b': 1', '1', '1, '(b)(i) transferring the hydrolysis liquid effluent for a period of time to a methanogenesis tank comprising fixed film methanogens or granular methanogens and producing biogas and methanogenesis tank liquid effluent;'}{'b': 1', '2', '1', '2', '2, '(b)(ii) transferring the methanogenesis tank liquid effluent to a methanogenesis tank comprising fixed film methanogens or granular methanogens during period of time and producing biogas and methanogenesis tank liquid effluent in methanogenesis tank ;'}{'b': 2', '1, '(b)(iii) discharging methanogenesis tank liquid effluent during period of time ;'}{'b': 1', '2, '(c) and then switching the order of methanogenesis tanks and and'}{'b': 2', '2', '2', '2, '(c)(i) transferring the liquid hydrolysis effluent for a period of time to said methanogenesis tank and producing biogas and methanogenesis tank liquid effluent in methanogenesis tank ;'}{'b': 2', '1', '2', '1', '1, '(c)(ii) transferring the methanogenesis tank liquid effluent to said methanogenesis tank during period of time and producing biogas and methanogenesis tank liquid effluent in methanogenesis tank ;'}{'b': 1', '2, ...

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16-02-2017 дата публикации

CONTAINER ASSEMBLY

Номер: US20170045422A1
Автор: GILES William
Принадлежит: BECTON, DICKINSON AND COMPANY

A container assembly such as a petri dish or a contact plate for use as a sampling device for microorganisms includes a base member, a lid and a locking mechanism that provides a secure locking engagement between the lid and the base member. The locking mechanism is designed such that does not lock except upon application of a specific intentionally applied compressive force, and which may be readily disengaged from locking engagement without the need for a rotational movement or torsional force. 14-. (canceled)5. The container assembly of claim 15 , wherein said base further comprising a flange extending in an outward direction from said side wall of said base claim 15 , wherein each said base latch member is located on a peripheral edge of said flange.6. The container assembly of claim 15 , wherein each said base latch member further comprises a ramp section claim 15 , an undercut and a narrow section base.7. The container assembly of claim 6 , wherein each said lid key member further comprisesa rib section having a first end and a second enda vertical stop wall at each of said first and second end of said rib section wherein the vertical stop wall prevents said lid key member from interengagement with the base latch member by rotational force.8. The container assembly of claim 7 , wherein each said rib section is held in locking engagement by said undercut of each said latch member.911-. (canceled)12. The container assembly of claim 15 , wherein said two pairs of locking members are radially spaced approximately 180 degrees apart.13. The container assembly of claim 15 , wherein said base member comprises a growth media.14. The container assembly of claim 15 , wherein said growth media comprises Tryptic Soy Agar.15. A container assembly for use in sampling a surface for the presence of microorganisms claim 15 , comprising:a base having a bottom wall, a continuous side wall, a flange extending outwardly from said continuous side wall, and at least two latch members ...

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19-02-2015 дата публикации

Laboratory Incubator Having Improved Moisture Distribution

Номер: US20150050725A1
Принадлежит:

The present invention relates to a laboratory incubator, more particularly, a gassing incubator, comprising an outer housing incorporating a door and an inner housing surrounding an internal chamber, which comprises a floor, a ceiling, three side walls and a lateral surface that is capable of being closed by said door or by an additional, inner door, and in which a flow channel is present, which comprises at least one air inlet opening in an end region and at least one air outlet opening in a different end region, wherein said at least one air outlet opening is formed such that effluent air is guided along at least one of the side walls and/or the lateral surface in the peripheral region of the internal chamber, and wherein a water reservoir capable of being heated is disposed within the flow channel such that air flowing in the flow channel is passed across or through said water reservoir. 1. A laboratory incubator , comprising:an outer housing incorporating a door, andan inner housing surrounding an internal chamber, which inner housing comprises a floor, a ceiling, three side walls and a lateral surface that can be closed by said door, said laboratory incubator further comprising a flow channel that comprises at least one air inlet opening in an end region and at least one air outlet opening in a different end region, wherein said at least one air outlet opening is formed such that effluent air is guided along at least one of the side walls and/or the lateral surface in the peripheral region of said internal chamber, and wherein a water reservoir capable of being heated is disposed within said flow channel such that air flowing in the flow channel is passed across or through said water reservoir.2. The laboratory incubator according to claim 1 ,wherein said flow channel is disposed between said outer housing and said inner housing.3. The laboratory incubator according to claim 1 ,wherein said at least one air outlet opening is disposed in a peripheral region of ...

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03-03-2022 дата публикации

FUNCTIONALIZED SUBSTRATE TO MANIPULATE CELL FUNCTION AND DIFFERENTIATION

Номер: US20220064581A1
Принадлежит:

The invention relates to a scaffold for steering cells into a predetermined direction of cell functionality, preferably a cell differentiation scaffold for steering cells into a predetermined direction of cell differentiation. The scaffold comprises a polydimethylsiloxane (PDMS)-, or rubber-, or silicone-based polymeric surface, and one or more cell functionality-inducing stimuli, preferably one or more cell differentiation-inducing stimuli, coupled to the polymeric surface.

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08-05-2014 дата публикации

BIOREACTOR CHAMBER

Номер: US20140127798A1
Принадлежит:

A bioreactor chamber assembly comprising a plurality of components interconnected together via interengaging formations to provide a bioreactor chamber that is assembled rapidly and having reliable intercomponent seals. A variety of different biological samples may be cultured within the chamber in vitro. By configuring the chamber with both liquid and a gas inlets and outlets, a controlled gas-liquid interface is created within the chamber to simulate certain in vivo cell and tissue environments. 1. A bioreactor chamber assembly comprising:a chamber body having walls that define an internal chamber to accommodate a biological sample;a liquid inlet to allow a liquid to flow into the internal chamber;a liquid outlet to allow a liquid to flow out from the internal chamber;a gas inlet to allow a gas to flow into the internal chamber; anda gas outlet to allow a gas to flow out from the internal chamber;the assembly configured to allow a flow of liquid through the internal chamber via the liquid inlet and outlet and to allow a flow of gas through the internal chamber via the gas inlet and outlet;wherein the assembly is configured such that a gas-liquid interface maybe maintained within the internal chamber between the gas and liquid as the gas and liquid flow through the internal chamber.2. The assembly as claimed in wherein the chamber body comprises at least a first component and a second component having respective interengaging formations that enable the first and second components to be releasably coupled together to define the internal chamber.3. The assembly as claimed in wherein the first component is a cap and the second component is a base having a foot portion to support the chamber body in an upright orientation when standing upon a support structure.4. The assembly as claimed in wherein the cap comprises a window to allow the internal chamber to be viewed externally.5. The assembly as claimed in any one of to wherein the interengaging formations project ...

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08-05-2014 дата публикации

BIOREACTOR CHAMBER

Номер: US20140127799A1
Принадлежит:

A bioreactor chamber assembly comprising a cap and a base capable of being coupled together axially to define an internal chamber. The cap and base comprise interengaging formations that are coupled together by a twist-lock rotation of the base and cap to provide a fluid tight seal between the base and cap via respective sealing surfaces. 1. A bioreactor chamber assembly comprising:a base and a cap configured to be coupled together to define an internal chamber;the base and cap each comprising a plurality of respective interengaging flanges to allow the base and cap to be realisably coupled together axially relative to a longitudinal axis bisecting the base and cap by rotation of at least one of the base and cap about the longitudinal axis;the flanges at each of the base and cap being respectively spaced apart in a direction around the longitudinal axis by gap regions configured to be occupied by at least a portion of the flange of the respective opposing base or cap, each flange having an abutment surface extending radially relative to the axis, the abutment surfaces at the base configured to slide over the abutment surfaces at the cap in touching contact by rotation of at least one of the base and cap about the axis such that at least a part of the flange of the base and cap overlap in the axial direction to couple and lock the base and cap axially relative to one another;the base and cap each comprising a respective sealing surface that may be drawn and mated together axially via an interference fit arrangement by said rotation and the contact between the abutment surfaces to provide a fluid tight seal when the base and cap are locked together axially.2. The assembly as claimed in claim 1 , wherein the respective interengaging flanges project radially outward from at least one of the base and cap.3. The assembly as claimed in claim 1 , wherein the respective interengaging flanges are spaced apart circumferentially around the base and cap.4. The assembly as ...

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08-05-2014 дата публикации

Control of carbon dioxide levels and ph in small volume reactors

Номер: US20140127802A1

Strategies to control the level of dissolved carbon dioxide (CO 2 ) concentrations and/or pH in small volume reactor chambers, and associated articles, systems, and methods, are generally provided. In certain embodiments, the reactor chambers can be configured to contain at least one biological cell.

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14-02-2019 дата публикации

PERFUSION BIOREACTOR AND METHOD FOR USING SAME TO PERFORM A CONTINUOUS CELL CULTURE

Номер: US20190048305A1
Принадлежит: CORNING INCORPORATED

The present disclosure generally relates to the bioprocess field and, in particular, to a perfusion bioreactor and a method for using the perfusion bioreactor for performing a continuous cell culture. 1. A perfusion bioreactor comprising:a vessel having a housing comprised at least in part of gas permeable membrane, at least one opening and a cavity;at least one lid attachable to the vessel to cover the at least one opening;a porous membrane disposed within the cavity to divide the cavity into an inner compartment and an outer compartment;a fresh media port extending through the vessel or the at least one lid;a spent media port extending through the vessel or the at least one lid; and,a mixer.2. The perfusion bioreactor of wherein the mixer comprises an impeller and a shaft claim 1 , wherein the impeller and the shaft are disposed within the inner compartment.3. The perfusion bioreactor of wherein the lid is removably attached to the vessel.4. The perfusion bioreactor of wherein the vessel or the porous membrane or both are flexible.5. The perfusion bioreactor of claim 1 , further comprising a gas sparger port extending through the vessel or the at least one lid.6. The perfusion bioreactor of further comprising a bleed-off port extending through the vessel or the at least one lid.7. The perfusion bioreactor of claim 1 , further comprising a sensor port extending through the vessel or the at least one lid.8. The perfusion bioreactor of claim 1 , wherein the vessel or the at least one lid further comprises a vent in communication with the cavity.9. (canceled)10. The perfusion bioreactor of claim 1 , wherein the porous membrane is attached to an opening within an inner vessel claim 1 , and wherein the inner vessel is disposed within the cavity of the vessel.11. The perfusion bioreactor of claim 10 , wherein the inner vessel further comprises one or more air exchange windows.12. The perfusion bioreactor of claim 1 , wherein the porous membrane having pores therein with ...

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25-02-2016 дата публикации

BIOREACTORS AND USES THEREOF

Номер: US20160053213A1
Автор: Hedberg Herbert

Aspects of the disclosure relate to bioreactors for maintaining biological objects (e.g., organ or engineered tissues) under culture conditions. In some embodiments, bioreactors and cover assemblies are provided that comprise a support base for supporting a biological object. In some embodiments, bioreactors and cover assemblies are provided that comprise a movable support base configured such that a user can manipulate its position and thus the relative position of the biological object. 1. A bioreactor comprising:a vessel having a chamber and at least one opening that communicates with the chamber, the chamber being configured for containing a biological object under culture conditions;a cover component configured for covering the at least one opening; anda support structure attached to the cover component, the support structure being configured for supporting the biological object when the cover component is separated from the vessel and for positioning the biological object within the chamber when the cover component is joined with the vessel to cover the at least one opening.2. The bioreactor of further comprising at least one port provided in the cover component claim 1 , the at least one port configured for providing access to the chamber while the cover component is joined with the vessel to cover the at least one opening.3. The bioreactor of further comprising a first fluid conduit attached to the at least one port such that the first fluid conduit is disposed in the chamber when the cover component is joined with the vessel to cover the at least one opening.4. The bioreactor of claim 3 , wherein the first fluid conduit is configured for attachment to a second fluid conduit of the biological object.5. The bioreactor of or claim 3 , wherein the first fluid conduit is configured for attachment to a second fluid conduit of the biological object through the use of one or more sutures.6. The bioreactor of any preceding claim wherein the support structure ...

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