УСТРОЙСТВО ДЛЯ БИОПЕЧАТИ ОДИНОЧНЫМИ ТКАНЕВЫМИ СФЕРОИДАМИ И ИСПОЛЬЗУЕМАЯ В НЕМ ПЕЧАТАЮЩАЯ ГОЛОВКА

Группа изобретений относится к области биотехнологии. Предложена печатающая головка и устройство печати тканевыми сфероидами. Печатающая головка включает систему каналов, содержащую входной и выходной каналы, верхний и нижний каналы сепарации питательной среды, а также канал для подпирающего плунжера. Входной канал имеет диаметр, обеспечивающий перемещение сфероида по нему только по одному. Выходной канал имеет диаметр не меньше диаметра входного канала, включает входное отверстие для ввода печатающего инструмента и выходное отверстие для вывода сфероидов. Каналы сепарации выполнены с возможностью соединения приспособления для отвода питательной среды с выходным каналом через систему микроканалов. Устройство включает печатающую головку, устройство подачи сфероидов с питательной средой во входной канал, приспособление для отвода питательной среды, подпирающий плунжер, печатающий инструмент, систему регистрации положения сфероида в выходном канале и вычислительное устройство для управления ...

УСТРОЙСТВО ОТБОРА ПРОБ СУБСТРАТА ДЛЯ АДГЕЗИОННОЙ КЛЕТОЧНОЙ КУЛЬТУРЫ

Verfahren zur Inokulation eine Flüssigkeit, und Inokulationsgefäss und Vorrichtung für die Anwendung dieses Verfahrens

Inkubatorvorrichtung und Verfahren

Inkubatorvorrichtung (10; 110), enthaltend eine Inkubatorkammer (112), eine Probenaufnahme (115), welche dazu eingerichtet ist, eine Probe (114) aufzunehmen und im Inneren der Inkubatorkammer (112) an einer Probenposition (P) zu positionieren, eine Heizanordnung mit mindestens einem Heizelement (140), eine Befeuchtungseinrichtung (116) zur Erzeugung eines Befeuchtungsfluids, sowie eine zwischen der Befeuchtungseinrichtung (116) und dem Inneren der Inkubatorkammer (112) angeordnete Übertrittsöffnung (160, 162), durch die das Befeuchtungsfluid entlang einer Übertrittsströmungsrichtung in gerichteter Strömung in das Innere der Inkubatorkammer (112) übertritt, dadurch gekennzeichnet, dass das mindestens eine Heizelement (140) innerhalb der Inkubatorkammer (112) angeordnet ist und Wärme an das Innere der Inkubatorkammer (112) abgibt, und dass die Übertrittsströmungsrichtung in einer von der Probenposition (P) wegführenden Richtung verläuft.

Vorrichtung zum Züchten von Keimkulturen

Die Erfindung betrifft eine Vorrichtung zum Züchten von Keimkulturen, mit einer Schale (1) und einem relativ zu dieser drehbaren Deckel (5), die beim Züchten der Keimkulturen ein geschlossenes System bilden, wobei auf den Boden (2) der Schale ein Nährboden (10) für die Keime aufgebracht ist und Mittel (13) zum Beimpfen des Nährbodens mit Keimen vorgesehen sind. DOLLAR A Es ist Aufgabe der Erfindung, eine derartige Vorrichtung so weiterzubilden, daß sie baulich einfach gestaltet ist und unkompliziert gehandhabt werden kann sowie der Anwendungsbereich der Vorrichtung wesentlich erweitert ist. DOLLAR A Gelöst wird die Aufgabe dadurch, daß der Deckel mit einer Öffnung (12) zur Aufnahme des Mittels zum Impfen des Nährbodens versehen ist, wobei das Mittel zum Beimpfen als Rechen (13), Kamm oder Bürste ausgebildet ist, das in den Deckel eingesetzt wird, wobei die Zinken (17) des Rechens bzw. Kamms oder die Borsten der Bürste bei mit der Schale verbundenem Deckel und bei in den Deckel eingesetzter ...

Körperflüssigkeitensensor und Verfahren zur Vermessung der Eigenschaften von Körperflüssigkeiten

Es werden ein Körperflüssigkeitensensor und ein Verfahren zur Vermessung einer Körperflüssigkeit angegeben, umfassend: ein Gehäuse zur Aufnahme einer Zellkultur mit lebenden Zellen, Mittel zur Zuführung der Körperflüssigkeit zu den Zellen der Zellkultur, wenigstens einen Sensor zur Messung von physiologischen Veränderungen der Zellen durch eine Beaufschlagung mit der Körperflüssigkeit, einen Zufluss zur Zuführung eines flüssigen Zellkulturmediums zur Aufnahmeeinheit, einen Abfluss zur Abführung des flüssigen Zellkulturmediums von der Aufnahmeeinheit, dadurch gekennzeichnet, dass der Sensor ein Sensor für metabolische Parameter der Zellen ist.

Behälter mit flexibler Wandung

Die Erfindung umfasst einen Behälter (1) mit flexibler Wandung (2) und mindestens einem durch einen Wandungsdurchbruch (7) in den von der flexiblen Wandung (2) umgebenen Behälterinnenraum (3) hineinragenden Entleerungsrohr (4, 4´, 4´´), dessen freies Ende (12) eine Entleerungsöffnung (13) aufweist, die in ihrer Lage in vertikaler Richtung auf unterschiedliche Entleerungshöhen (14) einstellbar ist. Der Behälter (1) zeichnet sich dadurch aus, dass in dem Wandungsdurchbruch ein Adapterstück (8) angeordnet ist, in dem das Entleerungsrohr (4, 4´, 4´´) längsverschieblich gehaltert ist und dass das Entleerungsrohr (4, 4´, 4´´) in einem Teilbereich von einer flexiblen Umhüllung (5) umgeben ist, die an einem ersten Ende (11) mit dem Entleerungsrohr (4, 4´, 4´´) und an ihrem dem ersten Ende (11) abgewandten zweiten Ende (10) mit der Wandung (2) oder dem Adapterstück (8) verbunden ist.

Biomass fermentation basin for gas production comprises a shallow downward entry ramp for vehicles serving for filling or emptying of the basin

Biomass fermentation basin for gas production, comprising a shallow downward entry ramp for vehicles serving for filling or emptying of the basin, is new.

Austragsvorrichtung für ein Beschickungssystem zum Bereitstellen von Biomasse in einer Biogasanlage

Die Erfindung betrifft eine Austragsvorrichtung (26) für ein Beschickungssystem (10), das zum Beschicken einer Biogasanlage mit Biomasse ausgelegt ist, umfassend einen Anschlusskörper (28) mit einer Schnittstelle für eine Antriebseinrichtung (42) des Beschickungssystems (10), mittels welcher die Austragsvorrichtung (26) um eine Rotationsachse (40) in Rotation versetzbar ist; zumindest einen sich in radialer Richtung von dem Anschlusskörper (28) nach außen erstreckenden Austragsarm (30) zum Befördern der Biomasse zu einer Austragsöffnung (22) des Beschickungssystems (10), wobei der Austragsarm (30) einen starr mit dem Anschlusskörper (28) verbundenen Hauptarm (32) und einen im Bereich eines vom Anschlusskörper (28) abgewandten Längsende des Hauptarms (32) angeordneten Armfortsatz (34) aufweist, der um eine parallel zur Rotationsachse (40) verlaufende Drehachse (44) verdrehbar am Hauptarm (32) gelagert ist; und ein Rückstellelement (46), welches ein Rückstellmoment auf den Armfortsatz (34 ...

Method and apparatus for performing 3-dimensional antibiotic susceptibility tests

Automatic device for inoculating a same sample at different concentrations

Heatseal sampling

Effects of space travel on human brain cells

Effects of space travel on human brain cells

A method of perfusing cells comprising: a) providing a fluid source, said fluid source in fluidic communication with a microfluidic device having at least one microchannel comprising surfaces, wherein at least one surface comprise living vascular cells; and b) contacting said vascular cells with said fluid at a flow rate of between 300 and 1500 μl per hour; and optionally, c) further comprising collecting at least a portion of the fluid that exits said microfluidic device; and optionally said flow rate of step b) is applied for at least 6 days. The cells may be brain endothelial cells, derived from fibroblasts and be human cells. A method of seeding such vascular cells in a microfluidic device is further claimed.

PROCESSING BIOMASS

PROCESSING BIOMASS

Algae growth for biofuels

Processing biomass.

Method for harvesting algae or plants and device used thereby

Algae growth for biofuels.

Process of production of filamentous mushroom spores.

Processing biomass.

PROCESSING BIOMASS

Processing biomass.

PROCESSING BIOMASS

Method for harvesting algae or plants and device used thereby

BAG WITH CULTURE AS WELL AS SAMPLING PROCEDURE BRED THEREIN

GÄRBZW. SEPTIC TANK

DEVICE TO THE WITHDRAWAL OF NUCLEAR SAMPLES FOR FABRIC ARRAY

MORE BESCHICKER FOR BIOMASS

ANMAISCHBEHÄLTER MIT MEHREREN RÜHRWERKEN

The mashing container includes a bottom portion (5), a container wall (3), a ceiling (7), and a vertically installed agitator (11). A filling opening is formed in the ceiling, and the agitator is positioned with respect to the filling opening such that the container is re-fed to a substrate. When the fill level of transported slurry container is above a predetermined level, the containers are transported away through the filling hole by the forced mixing of the contents of the container. An independent claim is included for method of operating agitator of mashing container.

MICRO-BIOLOGICAL TESTING DEVICE.

BIOLOGICAL GAS FACILITY

ELECTRODE ARRANGEMENT FOR FIELD CAGES

PROCEDURE AND DEVICE FOR THE MICRO PARTICLE POSITIONING IN FIELD CAGES

ELECTRODE ARRANGEMENT FOR DIELEKTROPHORETI PARTICLE DIVERSON

APPARATUS AND PROCEDURE FOR DIELEKTROPHORESIS

Methods for cell enrichment and isolation

Disclosed is a method of bioprocessing includes the steps of combining a suspension comprising a population of cells with magnetic beads to form a population of bead-bound cells in the suspension, isolating the population of bead-bound cells on a magnetic isolation column, and collecting target cells from the population of cells. Collecting the target cells includes dislodging bead-bound cells from the isolation column with an air plug. Also disclosed is a system including magnetic field generator, a magnetic cell isolation holder,a disposable fluidic kit and related methods of their use.

Vascularized in vitro arrays of living cells

High-throughput column arrays of vascularized living parenchyma / tissue having pillars dispersed in specialized configurations and arrangements substantially vertically through the column to provide support, passive or active perfusion, and access to internal portions of tissue for analytical sampling needs, along with 3-D printing methods of manufacture and analytical screening methods employing the column arrays.

Processing biomass

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material.

Magnetic immobilization of cells

Architecture tool and methods of use

Biomass hydrothermal decomposition system, sugar solution production method using biomass starting material, and alcohol production method using biomass starting material

A biomass hydrothermal decomposition system comprising: a biomass supply unit (12) that supplies a biomass starting material (11) under atmospheric pressure to elevated pressure, said biomass starting material (11) containing cellulose, a hemicellulose, and lignin; a hydrothermal decomposition unit (17) that hydrothermally decomposes the biomass starting material (11) using pressurized hot water (15) (hereinafter referred to as "hot water" too) so as to dissolve the lignin component and the hemicellulose component in the pressurized hot water (15); a biomass-solid extraction unit (18) that extracts biomass solids (20) from the hydrothermal decomposition unit (17); an enzymatic liquefaction tank (21) that communicates with the biomass-solid extraction unit (18) and, when the extracted biomass solids (20) are added, supplies an enzyme (30) to the biomass solids (20) to liquefy the biomass solids (20); and a discharge unit (23) that discharges the thus liquefied biomass solids (24) under elevated ...

Photobioreactor for liquid cultures

Culturing station for microfluidic device

A station for culturing biological cells in a microfluidic device is provided. The station includes one or more thermally conductive mounting interfaces, each mounting interface configured for having a microfluidic device detachably mounted thereon; a thermal regulation system configured for controlling a temperature of microfluidic devices detachably mounted on the one or more mounting interfaces; and a media perfusion system configured to controllably and selectively dispense a flowable culturing media into microfluidic devices detachably mounted on the one or mounting interfaces.

Method and apparatus for concentrating and/or positioning particles or cells

SYSTEM AND METHOD FOR TREATING WASTEWATER VIA PHOTOTACTIC HETEROTROPHIC MICROORGANISM GROWTH

A wastewater treatment method (200) includes the steps of supplying a wastewater influent (206) having nutrients for a population of phototactic heterotrophic microorganisms (204) to an aerated bioreactor (202). At least a portion of the nutrients is converted into the phototactic heterotrophic microorganism population (204). The wastewater influent (206) and the phototactic heterotrophic microorganism population (204) together form a bioreactor effluent (210) that is transferred to a light clarifier (212). The bioreactor effluent (210) is concentrated in the light clarifier (212) to form a low- solids effluent (216) and a high-solids effluent (214). The concentration is performed by inducing the population of phototactic heterotrophic microorganisms (204) to phototactically self concentrate through exposure to a light having at least one of an intensity and a wavelength sufficient to cause the phototactic heterotrophic microorganism population (204) to migrate away from a source of the ...

METHOD AND APPARATUS FOR THE CONVERSION OF PREPARED REFUSE SEWAGE SLUDGE MIXTURES INTO EARTH SUBSTANCE BY BIOLOGICAL DECOMPOSITION

SELECTIVE PARTICLES TRANSFER FROM ONE DEVICE TO ANOTHER

A target particle transferring device is disclosed, which comprises: (a) a substrate with a thickness of T and a width of W, having top and bottom portions, the top portion having a top surface and the bottom portion having a bottom surface; (b) a notch structure formed in the bottom portion of the substrate, comprising: a groove with a width of W1, located at a distance of t below the top surface of the substrate, wherein the groove is formed in the bottom portion from the bottom surface extending toward the top portion; and (c) a target substrate portion with a width of W2 and a thickness of T, located in the top and bottom portions of the substrate and being surrounded by the groove. Methods of transferring a target particle from one device to another is also disclosed.

METHODS AND SYSTEMS FOR PRINTING BIOLOGICAL MATERIAL

The present disclosure provides methods and systems for printing a three-dimensional (3D) material. In some examples, a method for printing a 3D biological material comprises providing a media chamber comprising a medium comprising (i) a plurality of cells and (ii) one or more polymer precursors. Next, at least one energy beam may be directed to the medium in the media chamber along at least one energy beam path that is patterned into a 3D projection wherein the x, y, and z dimensions may be simultaneously accessed in accordance with computer instructions for printing the 3D biological material in computer memory, to form at least a portion of the 3D biological material comprising (i) at least a subset of the plurality of cells, and (ii) a polymer formed from the one or more polymer precursors.

MICROCHIP FOR CELL ALIGNMENT AND METHOD OF CELL ALIGNMENT

... [PROBLEMS] To provide a microchip for cell alignment whereby cells can be quickly and accurately transferred and aligned with the combined use of the advantages of a system using optical tweezers and another system using micr ochannels. [MEANS FOR SOLVING PROBLEMS] A microchip for cell alignment which is provided, on a single baseboard, with: a cell injection channel which ha s a cell injection port connected to a liquid feeder mechanism and through w hich cells injected from the cell injection port migrate; a cell discharge c hannel which has a cell discharge port connected to a drainage mechanism and through which cells migrate toward the cell discharge port; a pooling part which is formed between the cell injection channel and the cell discharge ch annel and in which cells in the mid-course of flowing from the cell injectio n channel to the cell discharge channel are pooled; a cell alignment part in to which the cells pooled in the pooling part are introduced; and a guiding branch for ...

SYSTEM FOR AUTOMATED EXPLANT PREPARATION AND METHOD OF USE

A system and method for the automated or semi-automated preparation of explants for transformation and transgenic engineering.

SYSTEM OF MULTIPLE BAGS AND METHOD FOR THE PREPARATION OF HEMOCOMPONENTS

The present invention relates to a biomedical device for the production, storage, traceability and administration of blood components.

DEVICE AND APPARATUS FOR COLLECTING MICROBIAL GROWTH FROM A SEMI-SOLID SURFACE

A device for collecting a biological sample from a semi-solid surface. The device has a shaft with a proximate end and a distal end and a tip integrated with the shaft at the proximate end. The tip has a surface adapted to collect microorganisms thereon or release microorganism from, or both, wherein the adapted surface comprises at least one feature of a recess or extension to increase surface area of the tip and collect microorganisms thereon. Examples of such features include microfeatures with dimensions of about 1000 µm or less. Other examples include a pipette tip.

CULTURING STATION FOR MICROFLUIDIC DEVICE

A station for culturing biological cells in a microfluidic device is provided. The station includes one or more thermally conductive mounting interfaces, each mounting interface configured for having a microfluidic device detachably mounted thereon; a thermal regulation system configured for controlling a temperature of microfluidic devices detachably mounted on the one or more mounting interfaces; and a media perfusion system configured to controllably and selectively dispense a flowable culturing media into microfluidic devices detachably mounted on the one or mounting interfaces.

METHOD FOR PICKING UP CELL MATERIAL AND ASSEMBLY FOR PERFORMING SAID METHOD

In a method for picking up cell material from a culture dish a picking tool is displaced towards the cell material. Contact between the tool and the cell material is determined by carrying out capacitive measurements. After contact the tool is removed from the cell material. In an assembly for performing said method a support made of electrically conducting material supports the dish. A measurement device determines the start electrical capacity of the system composed of picking tool and support in a starting position of the tool, and determines the electrical capacity of the system when lowering the tool towards the dish. The measurement device provides signals representing the electrical capacity to a controller, which comprises a comparator for performing a comparison of the electrical capacity during lowering of the tool with the start electrical capacity. The controller controls the positioning of the tool at least based on this comparison.

CELL CULTURE METHOD AND SYSTEM

The invention is comprised within automated devices for cell culture. The invention relates to a cell culture method and system which allows modifying in a controlled manner the density and the number of cells in a culture. Specifically, the present invention relates to an iterative method the number of iteration steps of which is modified depending on the demand for the density and the number of cells necessary for the culture duration.

BIOMASS HYDROTHERMAL DECOMPOSITION SYSTEM, SACCHARIDE SOLUTION PRODUCTION METHOD USING BIOMASS RAW MATERIAL, AND ALCOHOL PRODUCTION METHOD USING BIOMASS RAW MATERIAL

A biomass hydrothermal decomposition system comprising: a biomass supply unit (12) that supplies a biomass starting material (11) under atmospheric pressure to elevated pressure, said biomass starting material (11) containing cellulose, a hemicellulose, and lignin; a hydrothermal decomposition unit (17) that hydrothermally decomposes the biomass starting material (11) using pressurized hot water (15) (hereinafter referred to as "hot water" too) so as to dissolve the lignin component and the hemicellulose component in the pressurized hot water (15); a biomass-solid extraction unit (18) that extracts biomass solids (20) from the hydrothermal decomposition unit (17); an enzymatic liquefaction tank (21) that communicates with the biomass-solid extraction unit (18) and, when the extracted biomass solids (20) are added, supplies an enzyme (30) to the biomass solids (20) to liquefy the biomass solids (20); and a discharge unit (23) that discharges the thus liquefied biomass solids (24) under elevated ...

APPARATUS AND METHOD FOR HARVESTING AND DEWATERING OF MICROALGAE BIOMASS

An apparatus and method for separating, harvesting and primary dewatering microalgae biomass from a microalgae solution by destabilization thereof with addition of kinetic energy thereto is disclosed. The method to overcome the energetic barrier preventing a fluid-solid separation comprises injecting the microalgae solution in an electrolytic system comprising an electrocoagulation reactor generally comprising an anode module and a cathode module, the anodes and the cathode(s) being adapted to be electrically connected to perform electrolysis, thus separating, harvesting and primary dewatering microalgae biomass. Such process is generally achieved by providing a DC electric current, between the anodes and the cathode(s), to perform the separation of the biomass in the solution, in preparation the following process steps of for liquid/solid separation and primary dewatering.

BIOREACTOR

A bioreactor comprises a reactor vessel (1) having an inlet system for influent or a mixture of influent and recycled material and a reaction chamber located above this system. In order to produce an excellent distribution of the influent (which may be mixed with recycled material) without there being a risk of blockage and without excessive wear problems arising, the outflow openings of the influent inlet system (12) are at least partially tangentially oriented and the influent inlet system is located in a chamber (2) which is separated from the reaction chamber by a partition which has at least one radial slit (13), formed by two radial edge strips (4a; 4b) overlapping one another some vertical distance apart, which radial slit forms the connection between the said influent inlet chamber and the reaction chamber.

PRODUCTION OF MICROBIAL CELLULOSE USING A ROTATING DISK FILMBIOREACTOR

A new microbial cellulose with high water content and process for making the new microbial cellulose utilizes a trough containing a biological medium and a cellulose producing microorganism. A plurality of disks are partly submerged in the medium and moved through the medium. Cellulose is formed on the surface of the disks which is later harvested.

CELL/TISSUE CULTURING DEVICE AND METHOD

A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consists of a sterilisable transparent and/or translucent disposable container which may be at least partially filled with a suitable sterile biological cell and/or tissue cultu re medium and/or axenic inoculant and/or sterile air and/or required other sterile additives. The container has means for removing excess air and/or waste gases therefrom , and means for introducing the inoculant and/or culture medium and/or additiv es therein. The device is characterised by having a reusable harvesting means for enabling harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvest ed cycle may serve as inoculant for a next culture and harvest ...

VERFAHREN ZUM IMPFEN EINES AUF EINER UNTERLAGE SICH BEFINDENDEN NAEHRMEDIUMS UND BEHAELTER ZUR DURCHFUEHRUNG DIESES VERFAHRENS.

Für die Probeabnahme und den Versand bakteriologisch zu untersuchenden Materials bestimmtes Gerät

Laboratoriumsharpune

VERFAHREN UND EINRICHTUNG ZUM BIOLOGISCHEN ABBAUEN VON AUFBEREITETEM MUELLKLAERSCHLAMMGEMISCH ZU ERDSUBSTANZ.

Apparatus for handling petri dishes.

Um Petrischalen um 180° zu wenden und den Boden vom Deckel zu lösen, ist ein Drehhalter (8) vorgesehen, welcher zwei Positionen (23, 24) zur Aufnahme von Petrischalen besitzt. Indem der Drehhalter (8) um eine Drehachse (21) gedreht wird, werden die Petrischalen in den beiden Positionen (23, 24) vertauscht und gewendet. Sodann wird der Boden (10b) der oberen Petrischale abgesenkt und von einem Greifer (14) ergriffen.

Device in order to cultivate, to control and protect the development of an embryo in vitro.

Dispositivo (100) per coltivare, controllare e proteggere lo sviluppo di un embrione in vitro comprendente: almeno ununità di incubazione (3); almeno ununità di lavoro con microscopio (2); detta unità di lavoro con microscopio comprendendo almeno un microscopio (4) ed almeno un braccio incubatore (5) che si estende da detta unità di incubazione (3) a detto microscopio; detto braccio incubatore essendo in comunicazione di fluido con la detta unità di incubazione (3); almeno ununità di ingresso/uscita (1) del materiale biologico; detta almeno un unità di ingresso/uscita (1) del materiale biologico essendo in comunicazione di fluido con la detta unità di incubazione (3). Lunità di incubazione presenta almeno un sistema di movimentazione per prelevare un campione di materiale biologico dallunità di ingresso/uscita (1) e portarla in una posizione di lavoro in corrispondenza del microscopio e/o viceversa.

Device in order to cultivate, to control and protect the development of an embryo in vitro.

Dispositivo (100) per coltivare, controllare e proteggere lo sviluppo di un embrione in vitro comprendente; almeno ununità di incubazione (3); almeno ununità di lavoro con microscopio (2); detta unità di lavoro con microscopio comprendendo almeno un microscopio (4) ed almeno un braccio incubatore (5) che si estende da detta unità di incubazione (3) a detto microscopio; detto braccio incubatore essendo in comunicazione di fluido con la detta unità di incubazione (3); almeno ununità di ingresso/uscita (1) del materiale biologico; detta almeno un unità di ingresso/uscita (1) del materiale biologico essendo in comunicazione di fluido con la detta unità di incubazione (3). Lunità di incubazione presenta almeno un sistema di movimentazione per prelevare un campione di materiale biologico dallunità di ingresso/uscita (1) e portarla in una posizione di lavoro in corrispondenza del microscopio e/o viceversa.

Growing and harvesting micro-algae in liquid

The culture of micro-algae is grown in a closed liquid circuit, at a temperature maintained constant between two predetermined limits. The liquid is supplied with quantities of carbon dioxide and nutrient products as required for growth of the algae. The liquid circuit includes a transparent portion allowing solar radiation to act on the liquid flow over part of its length, promoting photosynthesis of the algae. Oxygen generated by this process is extracted from the circuit, and at predetermined intervals the algae produced by the growth process are removed from the closed circuit.

Procedure and device for the series sampling of biological samples.

Method and apparatus for producing a three-dimensional object.

Bei einem Verfahren zur Herstellung und/oder Bearbeitung eines dreidimensionalen Objekts mit einem Druckmaterial (M, N, O), das an einer Zielposition in Form von diskreten dreidimensionalen Druckmaterialelementen abgegeben wird, wird mittels einer Dosiervorrichtung (20) aus mindestens einem Vorratsbehälter, in welchem ein Druckmaterial (M, N, O) vorrätig gehalten ist, Druckmaterial aufgenommen. Das Druckmaterial wird zu einer in allen drei Raumdimensionen definierten Zielposition transportiert und dort in einer dosierten Menge auf ein Substrat (60) bzw. ein darauf angeordnetes oder darauf im Aufbau befindliches dreidimensionales Objekt aufgebracht, um ein Druckmaterialelement zu bilden. Das Bilden eines Druckmaterialelements wird solange wiederholt, bis das dreidimensionale Objekt vollständig aufgebaut und/oder bearbeitet ist. Durch die Verwendung einer Dosiervorrichtung (20) zur Aufnahme, zum Transport und zum Aufbringen von Druckmaterial können praktisch beliebige Druckmaterialien verarbeitet ...

DEVICE AND METHODS OF PRINTING BIOLOGICAL TISSUES AND ORGANS

ТРАНСПОРТИРОВОЧНОЕ УСТРОЙСТВО ДЛЯ БИОМАССЫ В ФЕРМЕНТЕРЕ ДЛЯ ПОЛУЧЕНИЯ БИОГАЗА

Предлагается транспортировочное устройство (20) для транспортировки биомассы в ферментере (2) для получения биогаза, а также снабженный им большой ферментер, которое, соответственно в котором обеспечивается достаточная транспортировочная способность для биогаза через транспортировочный канал (18). Это достигается за счет размещения транспортировочных подушек (24-i) на дне, боковых стенках и/или на потолке, соответственно крышке, транспортировочного канала. За счет периодического заполнения и снова опустошения транспортировочных подушек биомасса перемещается через транспортировочный канал.

PROCESSING OF BIOMASS

ТРАНСПОРТИРОВОЧНОЕ УСТРОЙСТВО ДЛЯ БИОМАССЫ В ФЕРМЕНТЕРЕ ДЛЯ ПОЛУЧЕНИЯ БИОГАЗА

Предлагается транспортировочное устройство для транспортировки биомассы в ферментере для получения биогаза, а также снабженный им большой ферментер, в котором обеспечивается достаточная транспортировочная способность для биогаза через транспортировочный канал. Это достигается за счет размещения транспортировочных подушек на дне, боковых стенках и/или на потолке, соответственно, крышке транспортировочного канала. За счет периодического заполнения и опустошения транспортировочных подушек биомасса перемещается через транспортировочный канал.

СПОСОБ ПОЛУЧЕНИЯ УРОЖАЯ ВОДОРОСЛЕЙ ИЛИ РАСТЕНИЙ И УСТРОЙСТВО ДЛЯ ЕГО ОСУЩЕСТВЛЕНИЯ

В заявке описан способ получения урожая водорослей и(или) растений в открытой системе непрерывного действия, в которой водоросли и(или) растения выращиваются на погруженной в воду подложке, отличающийся тем, что подложку перемещают в процессе роста водорослей или растений.

PROCESSING OF BIOMASS

Continuous and long-acting biological 3D printing device and printing method

Exocervical and endocervical cell sampling device

Cyclic Microfluidic Chip And Method Of Using The Same

Biogas residue extractor for biogas digesters

Anaerobic fermentation digester for organic matter - comprises insulated enclosure with two compartments

Boîte de culture

Boîte de culture comprenant un réceptacle 1 qui est recouvert d'un couvercle 2 et sur le fond duquel est déposée une couche d'un milieu de culture. Deux orifices 3, 4, ménagés sur les faces en regard du réceptacle 1 et du couvercle 2, permettent de mettre l'intérieur du réceptacle en communication avec l'extérieur, alors même que le réceptacle 1 est recouvert du couvercle 2. Analyses médicales.

METHOD FOR MAKING A TANK AND RESULTING TANK

Apparatus of preparation of the feet of tank or leavens

PROCESS FOR DIRECT INOCULATION FROM CONCENTRATED FERMENTS AND ASSOCIATED DEVICE

Procédé d'ensemencement en continu d'un produit alimentaire, notamment un produit laitier, avec des ferments, comprenant les étapes suivantes : - des ferments concentrés solides sont transformés en ferments concentrés liquides, - les ferments concentrés transformés sont injectés en continu dans un courant de liquide à ensemencer, caractérisé en ce que les ferments concentrés liquides sont transformés - par décongélation de ferments concentrés congelés, dans une enceinte à régulation de température, - ou par réhydratation de ferments concentrés lyophilisés.

ENCLOSED BIOPRINTING DEVICE

SAMPLING DEVICE OF CARROTS FOR TISSUE ARRAY

L'invention concerne un dispositif de prélèvement de carottes 1 destiné à la construction de « tissue array » du type comportant un poinçon de prélèvement 5 de carottes d'échantillon, un poinçon de carottage 6 pour la réalisation d'évidements dans un ou plusieurs blocs dits receveur et un moyen d'éjection 7 des carottes prélevées dans un ou plusieurs blocs receveurs en paraffine ou en tout milieu congelé ou non. Le poinçon de carottage 6 est monté de manière sensiblement coaxiale dans le poinçon de prélèvement 5, le poinçon de prélèvement étant en position externe, les deux poinçons 5, 6 étant mobiles en translation et/ou rotation l'un par rapport à l'autre, et le moyen d'éjection étant agencé pour éjecter les carottes de chaque poinçon.

Ambient air treatment method for underground parking area, involves authorizing exchanges between container's medium and air from environment, where micro-organisms developed in medium generate calcified elements from carbon dioxide

Digester to produce methane for rural power supplies - can reverse flow of liq. manure etc. to prevent blockages

PROCEDE DE REALISATION D'UN RESERVOIR ET RESERVOIR AINSI OBTENU

L'INVENTION CONCERNE UN PROCEDE DE REALISATION DE RESERVOIRS, NOTAMMENT POUR PRODUITS LIQUIDES OU SEMI-LIQUIDES, AINSI QUE LES RESERVOIRS OU CUVES DE TRAITEMENT AINSI REALISES. SELON L'INVENTION, ON CREUSE DANS LE SOL NATUREL UNE TRANCHEE, DE SECTION EVASEE VERS LE HAUT, ON MET EN PLACE SUR LES PAROIS PARALLELES INCLINEES 2, 2 DE LADITE TRANCHEE, AINSI QUE SUR LES PAROIS TRANSVERSALES D'EXTREMITE, DES ELEMENTS DE SURFACAGE 8, 8 JOINTIFS ASSURANT AINSI L'ETANCHEITE DE LA CAPACITE OUVERTE CONSTITUANT LA TRANCHEE, ON MET EN PLACE AU-DESSUS DE LA TRANCHEE AU MOINS UN, ET DE PREFERENCE UNE PLURALITE D'ELEMENTS DE COUVERTURE 15 REPOSANT PAR DEUX BORDS OPPOSES 13, 13 SUR LES DEUX BORDS PARALLELES DE LA TRANCHEE, CES ELEMENTS COMPORTANT AUX EXTREMITES TERMINALES UNE PAROI TRANSVERSALE D'OBTURATION, ON INTERPOSE ENTRE DEUX ELEMENTS DE COUVERTURE ADJACENTS DES MOYENS D'ETANCHEITE, ON SOLIDARISE L'ENSEMBLE DESDITS ELEMENTS DE COUVERTURE 15, SUR UN SUPPORT 10, 10 LUI-MEME SOLIDAIRE DES MOYENS DE SURFACAGE ...

PHOTOBIOREACTOR

The present invention relates to a photobioreactor capable of culturing photosynthetic microorganisms. According to an embodiment of the present invention, the photobioreactor includes: a culture container having an internal storage space for culturing photosynthetic microorganisms therein, and storing a culture solution in the storage space; a gas supply member supplying air to the culture container; a gas discharge member combined with the culture container to discharge the air, which has been supplied to the culture container and has gone through the culture solution, to the outside; a culture solution circulation member having an end connected to the culture container, and including a main line discharging the culture solution to the outside of the container and then resending the solution to the container, and a pump part provided to circulate the culture solution flowing in the main line; a spray member supplying the culture solution to the culture container by spraying the culture ...

APPARATUS FOR FEEDING AND DISCHARGING OF KOJI MAKING MACHINE

높이 조절이 가능한 헤링본 타입 유체유도유닛을 이용한 세포 농축 장치

... 세포 농축 장치가 개시된다. 본 발명의 일 실시 예에 따른 세포 농축 장치는 분류 대상 세포 또는 미세입자가 포함된 유체가 유동하는 유체채널, 상기 유체채널의 상단에 마련되고, 제공되는 공기압에 따라 형상이 변화되는 공기압 채널 및 상기 공기압 채널에 분류 대상 세포 또는 미세입자의 크기에 기초한 공기압을 제공하는 공기압 공급 채널을 포함하고, 상기 공기압 채널은 상기 공기압 공급 채널로부터 공급되는 공기압에 따라 형상이 변형되어 상기 분류 대상 세포 또는 미세입자 크기에 대응하는 높이를 갖는 헤링본 타입 유체유도유닛을 형성한다.

DEVICE AND METHOD FOR MICROFLUIDICS-BASED 3D BIOPRINTING

3차원 구조물의 첨삭 가공을 위한 장치 및 방법

... 3차원 세포 구조를 포함하는 3차원 구조물의 첨삭 가공을 위한 장치 및 방법이 제공된다. 상기 장치는 외피 유체(sheath fluid)와 하이드로겔(hydrogel)을 함유한 물질을 수용하여 분배하기 위한 적어도 하나의 프린트 헤드를 포함하고, 이때 상기 프린트 헤드는 물질을 분배하기 위한 오리피스, 상기 물질을 수용하여 챔버향성을 부여하기 위한 마이크로유체 채널, 상기 프린트 헤드에서 상기 마이크로유체 채널의 하나에 대응하고 상기 마이크로유체 채널에서 유체 유동을 허용 또는 금지하도록 구성된 유체 스위치들을 포함하며, 상기 장치는 상기 오리피스로부터 분배된 물질의 제 1 층을 수용하기 위한 수용 표면; 상기 프린트 헤드의 상기 오리피스를 3차원 공간에 위치시키기 위한 위치선정 유닛; 및 상기 프린트 헤드의 상기 오리피스로부터 물질을 분배하기 위한 분배수단을 또한 포함한다.

SYSTEM FOR PROCESSING OF HIGH VALUE PRODUCTS CO2 IN AGGREGATE AND/OR NUTRITIONAL AND OTHER FEATURES OF ENERGY

INTEGRATED BIOREFINERY

Sistemas y métodos para producir bioproductos. Los sistemas y métodos se pueden configurar y usar en una biorrefinería integrada. La biorrefinería integrada puede comprender una planta de producción de azúcar, por ejemplo un molino azucarero, una planta productora de uno o mas bioproductos, por ejemplo butanol, y opcionalmente una planta de producción de etanol, en las que se emplean el sistema y el método. Medio de cultivo.

Bioprinting station, assembly comprising such bioprinting station and bioprinting method

Bioprinting station (1) comprising:—a Bioprinting device (4) adapted to deposit a pattern of biological material (2) onto an area of interest (3 a ) of a substrate (3),—an imaging system (15) adapted to acquire an image of the substrate (3) and to reveal on the acquired image the area of interest (3 a ) with respect to a remaining part (3 b ) of the substrate (3), the acquired image of the substrate (3) being processed so as to detect the revealed area of interest (3 a ) on the acquired image and to determine the pattern corresponding to the area of interest (3 a ) detected on the acquired image.

Vessel with adjustable side drain

An apparatus for processing fluid includes a vessel provided with a sidewall including a passage and at least partially defining an interior compartment. A drain tube positioned in the interior compartment may drain fluid through the passage in the sidewall. The drain tube may be angled, and arranged such that the end in the interior compartment may be adapted for movement as the result of manipulation of a structure, such as a coupler, external to the interior compartment. Accordingly, the drain tube may be positioned along the sidewall at a reference point for movement between a first position for draining fluid above the reference point and a second position for draining fluid below the reference point. An indexing feature may also be provided for incrementally adjusting the position of the drain tube, along with an associated tool to facilitate the adjustment. Related methods are also disclosed.

System and method for high-voltage pulse assisted aggregation of algae

A method and device for aggregating algae in an aqueous solution is disclosed. The method can include providing an algae feed comprising a liquid and algae dispersed therein. The algae feed can be aggregated by applying a nanosecond pulsed electric field to the algae feed. The nanosecond pulsed electric field can include a plurality of electric pulses having a pulse duration ranging from 1 to 1,000 nanoseconds. The method can also include separating an aggregated algae stream from the algae feed and feeding the aggregated algae stream to a lipid extraction operation.

Systems and devices for restraining a micro-object and associated methods

Systems, devices, and methods for restraining a micro-object are provided. In one aspect, a micro-object restraint device is provided having a micro-barrier structure coupled to a support substrate, where the micro-barrier structure has two micro-object holding regions. Each micro-object holding region includes a micro-object receiving opening defined in the micro-barrier structure, a micro-object impeding opening at an internal region of the micro-barrier structure, and at least two contact points positioned adjacent to the micro-object impeding opening and oriented to contact and impede a micro-object at the micro-object opening. The two micro-object holding regions abut one another and the micro-object openings from the two micro-object holding regions are continuous.

Method and apparatus for growing photosynthetic organisms

A biological cultivation system for the culture of photosynthetic organisms including at least one cultivation chamber permitting exposure of the culture medium to natural and/or artificial light and including; a light transmissive wall or walls defining a gas space; and a culture medium containment area below the gas space; one or more fluid inlets positioned within the culture medium containment area; and one or more gas outlets in communication with the gas space; a control unit operatively connected to a gas flow control device, the gas flow control device controlling the flow of gas in through the fluid inlets and out through the fluid outlets to control the conditions within the cultivation chamber.

POST PROTEIN HYDROLYSIS REMOVAL OF A POTENT RIBONUCLEASE INHIBITOR AND THE ENZYMATIC CAPTURE OF DNA

The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample. 1. A method for collecting particles in a blood sample , the method comprising:(a) flowing blood treated with a fibrin lysis reagent across flexible electrodes constructed and arranged similarly to a fish gill, wherein the flexible electrodes have deposited thereon hyaluronic acid strands attached to bioactive peptides and wherein the fibrin lysis reagent comprises a nuclease, a plasminogen and a plasminogen activator; and(b) applying an electric potential via the electrodes to collect particles in the blood that are attracted to the bioactive peptides.2. The method of claim 1 , wherein the blood treated with a fibrin lysis reagent has been contacted with a metal claim 1 , resin claim 1 , and/or nanomaterial that attaches to bacteria in the blood.3. The method of claim 1 , wherein the bioactive peptides are attached to the hyaluronic acid strands via biotin and streptavidin.4. The method of claim 1 , wherein the hyaluronic acid strands are cross-linked utilizing biotin and streptavidin to form a hyaluronic acid matrix.5. The method of claim 4 , wherein the streptavidin is cross-linked.6. The method of claim 1 , wherein the particle is a bacterium.7. A device for collecting particles from bodily fluid claim 1 , the device comprising:(a) at least one layer;(b) flexible electrodes attached to the at least one layer; and(c) hyaluronic acid deposited on the electrodes, the hyaluronic acid configured to attract particles.8. The device of claim 7 , wherein the electrodes are arranged in a fish-gill like arrangement.9. The device of claim 7 , wherein the at least one ...

HIGH DENSITY SELF-CONTAINED BIOLOGICAL ANALYSIS

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR. 1. A container for performing a plurality of reactions on a fluid sample in a closed system comprisinga high density reaction zone provided in the closed system comprisinga channel for receiving the sample into the high density reaction zone,a plurality of high density reaction wells fluidly connected to the channel, each high density reaction well comprising one or more reagents, anda barrier layer that minimizes cross-contamination between high density reaction wells upon introduction of the sample to the high density reaction zone, wherein the barrier layer is a pierced layer having one or more piercings therethrough per reaction well, and wherein the piercings are large enough to allow the fluid sample to pass into the reaction well in the presence of a force, but small enough to substantially prevent the fluid sample from passing absent the force,wherein the high density reaction zone is configured such that the sample received through the channel flows across the pierced layer and over all of the piercings.2. The container of wherein the container comprises a flexible layer that can be pressed against the high density reaction wells to seal a portion of the sample in each of the plurality of wells.3. The container of wherein the force is vacuum in each of the high density reaction wells.4. The container of wherein the plurality of high density reaction wells comprises at least 50 high density reaction wells.5. The container of wherein the plurality of high density reaction wells comprises at least 120 high density reaction wells.6. The container of wherein the plurality of high density reaction wells comprises at least 400 high density reaction wells.7. The container of wherein the reagents comprise a pair of PCR primers.8. The container of ...

Post protein hydrolysis removal of a potent ribonuclease inhibitor and the enzymatic capture of dna

The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample.

Cell culture apparatus

The invention provides a unique cell culture chamber, as well as systems for culturing and analyzing cells, methods of using the chamber, and techniques for culturing and analyzing cells. The invention also provides an apparatus for distributing cells across a substrate, comprising a chamber comprised of at least one polydimethylsiloxane (PDMS) surface, where the chamber comprises one or more wells, and each well can contain a volume of a suspension of cells in culture, and where the chamber, when placed over a substrate, provides a more uniform dispersement of cells on the substrate than when the cells are deposited on a substrate without said chamber.

Microbial air sampler

An air sampler device has a top plate and a bottom plate, and receives a Petri dish between the top plate and the bottom plate. The top plate includes 283 substantially small holes. The bottom plate has a deepened center well formed in the top surface. Elongated slots are formed in the top surface which extend out from the well. The slots have distal ends which extend beyond the Petri dish. Air is drawn into the sampler by a vacuum tube through an air port which communicates with the center well. Air is pulled into the 283 holes in the top plate and strikes the capture material in the Petri dish. The air then travels up over the sides of the dish, into the distal ends, through the slots, and into the center well, where it exits out of the vacuum air port.

TRANSPLANTATION GUIDE AND TRANSPLANTATION DEVICE

The present invention provides a transplantation guide which is formed into a bottomed cylinder having a base material that allows communication of a culture fluid, wherein: 1. A transplantation guide of the present invention which is formed into a bottomed cylinder having a base material that allows communication of a culture fluid , wherein:a cell mass supplied from the open end side is placed on the base material, andthe guide is used as a mold for forming a three-dimensional cell construct by culturing the cell mass placed on the base material by immersing the base material side in an excessive amount of a culture fluid.2. The transplantation guide according to claim 1 , wherein the thickness of the cylinder is 0.05-0.3 mm.3. The transplantation guide according to either one of and claim 1 , wherein the base material is formed with a biodegradable material.4. The transplantation guide according to claim 3 , wherein the base material claim 3 , together with the three-dimensional cell construct claim 3 , transfers from the opening at the base material side to be retained at the recipient site.5. The transplantation guide according to claim 1 , which is formed with a transparent material.6. A transplantation device which holds the end of the transplantation guide according to at the open side claim 1 , wherein the transplantation guide is withdrawn from a recipient site while making the three-dimensional cell construct placed on the base material to be retained at the recipient site. The present invention relates to a transplantation guide for transplanting a three-dimensional cell construct into an affected area and a transplantation device for transplanting a three-dimensional cell construct into an affected area by using said transplantation guide.Recently, studies of regenerative medicine are making progress. For example, in regenerative medicine, artificially cultured cells are used instead of the damaged cells in an affected area for regenerative treatment of ...

Conditioning chamber for storing samples in a time-controlled manner and method for storing samples in a time-controlled manner

The invention relates to an air-conditioning space ( 10 ) for storing samples in a time-controlled manner, comprising a device ( 22 ) for automatically feeding sample containers ( 18 ) into a climatically sealed space ( 12 ) having at least one wall ( 43 ), into which a sample container ( 18 ) can be inserted into the climatically sealed space ( 12 ) through an opening ( 40, 42 ), wherein the feed device ( 22 ) has at least one drive and control unit, and an incubator receptacle ( 30 ) for receiving the samples ( 18 ) is provided inside the climatically sealed space ( 12 ). The invention is distinguished by the fact that the feed device has an automatic feed arm ( 22, 24 ) which grips a sample container ( 18 ) from a receiving position outside the climatically sealed space ( 12 ) and deposits the sample container ( 18 ) there in a clear deposit position in an incubator receptacle ( 30 ).

Processes and systems for the fermentative production of alcohols

The present invention relates to the fermentative production of alcohols including ethanol and butanol, and processes for improving alcohol fermentation employing in situ product removal methods.

Deposition device, and collection device

A Film ( 7 ) is provided on at least a part of a surface of each of a vapor deposition preventing plate ( 3 ) and a shutter ( 4 ) of a vacuum chamber ( 5 ) on which surface vapor deposition particles are vapor-deposited, the film ( 7 ) being provided so as to be peeled off from the each of the vapor deposition preventing plate ( 3 ) and the shutter ( 4 ), and the film being made of a material differing in at least one of a melting point, a sublimation point, solubility in a given solvent, microbial biodegradability, and photodegradability from a material of which a vapor-deposited film that is formed on the film ( 7 ) is made.

CELL CULTURE METHOD AND CELL CULTURE KIT

Provided is a means suitable for mass culture of adherent cells and which allows easy and safe collection of the cultured adherent cells. A cell culture kit has a culture bag having flexibility, such a level of hydrophilicity that the culture of adherent cells can be achieved, and permeability into a bag shape and which is filled with a culture medium; a dissociation solution bag filled with a dissociation solution containing a metal chelating agent or ornithine or a derivative thereof, and a solution sending circuit or a derivative thereof, and a solution sending circuit which connects the culture bag and the dissociation solution bag to each other and can send the dissociation solution from the dissociation solution bag to the culture bag. 1. A cell culture method of culturing adherent cells , comprising:a first step of holding a liquid culture medium in a culture bag which is formed by shaping at least one sheet having flexibility, such a level of hydrophilicity that culture of adherent cells can be achieved, and permeability into a bag shape, and sowing adherent cells to an inner surface having hydrophilicity of the at least one sheet of the culture bag, and incubating the same;a second step of discharging the culture medium from the culture bag, and then filling the culture bag with a dissociation solution containing a metal chelating agent or ornithine or a derivative thereof;a third step of bending the at least one sheet of the culture bag filled with the dissociation solution to dissociate the adherent cells from the at least one sheet; anda fourth step of collecting the adherent cells dissociated from the at least one sheet of the culture bag.2. The cell culture method according to claim 1 , wherein the dissociation solution contains EDTA.3. The cell culture method according to claim 1 , wherein claim 1 , in the third step claim 1 , the sheet of the culture bag is bent at an environmental temperature lower than an environmental temperature of the culture ...

SYSTEM AND METHOD FOR DETECTION AND SORTING OF CELLS

A system and method for detection of cells and sorting of cells are disclosed. Target cells, such as circulating tumor cells (CTCs) or antigen-specific antibody producing circulating memory B cells from COVID-19 patients, may be of interest. Magnetic beads may be bound to the target cells. After which, the bead-bound target cells may be identified using an applied magnetic field. In one example, magnetic sensors may be used to detect movement of the bead-bound target cells responsive to an applied magnetic field. In another example, an optical sensor may be used to detect movement of the bead-bound target cells responsive to an applied magnetic field. Further, separate from identification of the target cells, the bead-bound target cells may be sorted using an applied magnetic field. In this way, a magnetic field may be used for target cell identification and target cell sorting in order to detect and collect target cells of interest at the single-cell resolution. 1. An apparatus configured to determining whether a magnetic bead-labeled target cell is present in a fluid , the apparatus comprising:a well configured to house the fluid containing particles and including at least one outlet;at least one magnetic field generator configured to generate a magnetic field to at least a part of the well;one or more sensors configured to generate sensor data; and control the magnetic field generator to generate the magnetic field to the at least a part of the well;', 'identify, based on the sensor data responsive to the magnetic field, the magnetic bead-labeled target cell and an associated location within the well; and', 'control the magnetic field generator, based on the associated location within the well of the magnetic bead-labeled target cell and the at least one outlet, in order to move the magnetic bead-labeled target cell toward the at least one outlet, thereby sorting the magnetic bead-labeled target cell, in order to remove the magnetic bead-labeled target cell from ...

BIOPROCESSING METHODS FOR CELL THERAPY

A non-transitory computer readable medium includes instructions configured to adapt a controller to maintain a first target environment in a bioreactor vessel containing a population of cells for a first incubation period to produce a population of genetically modified cells from the population of cells, initiate a flow of media to the bioreactor vessel, maintain a second target environment in the bioreactor vessel for a second incubation period to produce an expanded population of genetically modified cells. 1. A non-transitory computer readable medium comprising instructions configured to adapt a controller to:maintain a first target environment in a bioreactor vessel containing a population of cells for a first incubation period to produce a population of genetically modified cells from the population of cells;initiate a flow of media to the bioreactor vessel; andmaintain a second target environment in the bioreactor vessel for a second incubation period to produce an expanded population of genetically modified cells.2. The non-transitory computer readable medium of claim 1 , wherein:the instructions are configured to adapt the controller to actuate at least one valve to place a reservoir containing the population of cells in fluid communication with the bioreactor vessel, and actuate at least one pump to pump the population of cells from the reservoir to the bioreactor vessel.3. The non-transitory computer readable medium of claim 2 , wherein: the instructions are configured to adapt the controller to actuate at least one valve to place a media bag in fluid communication the reservoir claim 2 , and actuate at least one pump to pump media from the media bag into the reservoir to rinse the reservoir claim 2 , and then transfer rinse media from the reservoir into the bioreactor vessel.4. The non-transitory computer readable medium of claim 1 , wherein:the second target environment includes a temperature within the bioreactor vessel and a carbon dioxide ...

Large-scale Bioreactor

In an embodiment of the invention, there may be provided a bioreactor having tissue scaffolds and having culture medium perfused therethrough. There may be multiple independent culture chambers and reservoirs or sub-reservoirs. Sensors can provide for individually controlling conditions in various culture chambers, and various culture chambers can be operated differently or for different durations. It is possible to infer the number of cells or the progress toward confluence from the fluid resistance of the scaffold, based on flowrate and pressure drop. Harvesting may include any combination or sequence of; exposure to harvesting reagent; vibration; liquid flow that is steady, pulsatile or oscillating; passage of gas-liquid interface through the scaffold. Vibration and flow can be applied so as to reinforce each other. 1. A bioreactor system for culturing cells ,said bioreactor system comprising spatially fixed scaffolds upon which said cells can grow,said bioreactor system having a liquid supply system for perfusing liquid through said scaffolds,wherein said bioreactor system comprises a plurality of culture chambers each containing some of said scaffolds, said culture chambers having respective flow paths therethrough for flow of said liquid,wherein said bioreactor system comprises a plurality of reservoirs or a plurality of sub-reservoirs,wherein said bioreactor system has a control device to direct, to various of said plurality of culture chambers at a given time, respective flows of said liquid that are different from flows to others of said culture chambers with respect to flowrate of said liquid or flow direction of said liquid or duration of flow of said liquid.2. The bioreactor system of claim 1 , wherein said control device comprises at least one sensing device selected from the group consisting of a pH sensor claim 1 , a dissolved oxygen sensor claim 1 , a glucose sensor claim 1 , a lactate sensor claim 1 , a camera claim 1 , and a device indicating a ...

METHODS AND COMPOSITIONS FOR DETACHING ADHERENT CELLS

This disclosure is directed to methods and systems for harvesting cells. 1. A method of detaching adherent cells from fibrous , three-dimensional (3D) carriers , comprising the steps of:incubating said adherent cells with an agent that disrupts adhesion of said adherent cells to said carrier; andsubjecting said 3D carriers to a rotary motion while said 3D carriers are submerged in an aqueous solution.2. The method of claim 1 , wherein said 3D carriers are disposed within a bioreactor chamber.3. The method of claim 1 , wherein said 3D carriers and said aqueous solution are disposed within a chamber claim 1 , and said rotary motion is imparted by protruding objects projecting radially from a central axis of said chamber.4. The method of claim 3 , wherein said protruding objects are rotated in a continuous rotary motion.5. The method of claim 3 , wherein said protruding objects are rotated in an oscillating rotary motion.6. The method of claim 1 , wherein said 3D carriers are packed within a basket claim 1 , and said rotary motion is imparted by rotation of said basket.7. The method of claim 6 , wherein said basket is disposed within an outer container claim 6 , said aqueous solution is present in said basket and said outer container claim 6 , and said basket is rotated relative to said outer container.8. The method of claim 7 , wherein said basket comprises porous walls.9. The method of claim 6 , wherein said rotation is a continuous rotation.10. The method of claim 6 , wherein said rotation is an oscillating rotation.11. The method of claim 1 , wherein said agent is removed prior to said subjecting said 3D carriers to a rotary motion.12. The method of claim 1 , wherein said agent is present during said subjecting said 3D carriers to a rotary motion.13. The method of claim 1 , wherein said agent comprises a protease.14. The method of claim 13 , wherein said agent further comprises a chelator of divalent ions15. The method of claim 1 , wherein said agent comprises a ...

DEVICES, SYSTEMS, AND METHODS FOR THE FABRICATION OF TISSUE

Described herein are bioprinters comprising: one or more printer heads, wherein a printer head comprises a means for receiving and holding at least one cartridge, and wherein said cartridge comprises contents selected from one or more of: bio-ink and support material; a means for calibrating the position of at least one cartridge; and a means for dispensing the contents of at least one cartridge. Further described herein are methods for fabricating a tissue construct, comprising: a computer module receiving input of a visual representation of a desired tissue construct; a computer module generating a series of commands, wherein the commands are based on the visual representation and are readable by a bioprinter; a computer module providing the series of commands to a bioprinter; and the bioprinter depositing bio-ink and support material according to the commands to form a construct with a defined geometry. 1. A bioprinter comprising:a) one or more printer heads, wherein each printer head comprises a means for receiving and holding at least one cartridge, and wherein each cartridge comprises a deposition orifice and a bio-ink, the bio-ink comprising a solid or semi-solid composition comprising living cells;b) a means for dispensing the bio-ink of a selected cartridge by application of pressure to extrude the bio-ink of the selected cartridge through the deposition orifice;c) a means for determining a position of the selected cartridge in space; andd) a programmable computer processor for regulating the pressure and the speed of the dispensing of the bio-ink communicatively coupled to the means for determining a position of the selected cartridge and the means for dispensing the bio-ink.2. The bioprinter of claim 1 , wherein the means for dispensing the bio-ink of a selected cartridge applies pressure via a piston claim 1 , compressed gas claim 1 , hydraulics claim 1 , or a combination thereof.3. The bioprinter of claim 1 , further comprising a means for adjusting ...

Method for Assessing a Compound Interacting with a Target on Epithelial Cells

Disclosed herein is a method for assessing a compound interacting with a target on polarized epithelial cells. The method comprising the steps of providing an organ chip comprising a main channel and polarized epithelial cells, wherein the main channel is divided into an apical channel and a basal channel separated by the polarized epithelial cells, wherein the apical side of the polarized epithelial cells is directed towards the apical channel and the basolateral side of the polarized epithelial cells is directed towards the basal channel. Determining the localization and optionally the expression level of the target on the polarized epithelial cells. Administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the basal channel, when the target is localized on the basolateral side of the epithelial cells or administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the apical channel, when the target is localized on the apical side of the epithelial cells. Measuring a parameter of the administration of the compound and the peripheral blood mononuclear cells.

INCUBATOR APPARATUS AND METHODS

Apparatus and methods for monitoring embryos in an incubator are described. The apparatus comprises an incubation chamber defined by an incubation chamber housing and a slide carrier comprising a plurality of compartment walls that define compartments for holding embryo slides within the incubation chamber for incubation. The slide carrier is moveable, for example by rotation, relative to the incubation chamber housing to allow a selected compartment to be moved to a loading position defined at least in part by a loading port wall associated with the incubation chamber housing. The loading port wall is arranged to cooperate with the wall of a compartment in the loading position to restrict the extent to which the environment of the compartment in the loading position is in fluid communication with the environments of other compartments in the incubation chamber. 1. An incubator for embryos supported on embryo slides , the incubator comprising:an incubation chamber defined by an incubation chamber housing; anda slide carrier comprising a plurality of compartment walls that define compartments for holding embryo slides within the incubation chamber for incubation, wherein the slide carrier is moveable relative to the incubation chamber housing to allow a selected compartment to be moved to a loading position defined at least in part by a loading port wall associated with the incubation chamber housing, and wherein the loading port wall is arranged to cooperate with the wall of a compartment in the loading position to restrict the extent to which the environment of the compartment in the loading position is in fluid communication with the environments of other compartments in the incubation chamber.2. The incubator of claim 1 , wherein the loading port wall is positioned to align with the compartment wall of a compartment in the loading position to restrict the extent to which the environment of the compartment in the loading position is in fluid communication with the ...

AUTOMATED DEVICES, SYSTEMS, AND METHODS FOR THE FABRICATION OF TISSUE

Described herein are improvements to bioprinting technology that facilitate automation of tissue and organ fabrication processes. 120.-. (canceled)21. A bioprinter comprising:(a) a printer head comprising at least one cartridge and at least one deposition orifice, the cartridge comprising contents from a bio-ink, a support material, or a combination thereof;(b) a receiving surface for receiving the contents from the printer head; (1) a tip triangulation sensor, fixed to the receiving surface, for determining a position of the deposition orifice, the tip triangulation sensor comprising a first laser and a first sensor configured to detect light from the first laser;', '(2) a surface triangulation sensor, fixed to the printer head, for determining a position of a target print surface, the surface triangulation sensor comprising a second laser and a second sensor configured to detect light from the second laser; and, '(c) a three-dimensional calibration system comprising (1) calculate one or more print height changes during bioprinting based on (a) the position of the deposition orifice determined from the tip triangulation sensor and (b) the position of the target print surface determined from the surface triangulation sensor, the print height is a distance between the deposition orifice and the target print surface; and', '(2) adjust for any print height changes during bioprinting by adjusting one or more bioprinting parameters., '(d) a processor coupled to the three-dimensional calibration system and configured to22. The bioprinter of claim 21 , wherein the bioprinting parameters are selected from the group consisting of a deposition rate claim 21 , a relative travel speed of the printer head claim 21 , the print height claim 21 , a cell type claim 21 , a deposition order claim 21 , a deposition location claim 21 , and combinations thereof.23. The bioprinter of claim 21 , wherein the print height changes are determined before claim 21 , after claim 21 , or before ...

Processing biomass

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material.

SYSTEM AND METHOD FOR BUOYANT PARTICLE PROCESSING

A system for buoyant particle processing includes: a reaction vessel, a stirring mechanism, a set of one or more pumps, and a filter. The system can additionally or alternatively include a set of pathways and/or any other suitable component(s). A method for buoyant particle processing includes: stirring the contents of a reaction vessel; washing a set of buoyant particles; and filtering the contents of the reaction vessel. Additionally or alternatively, the method can include any or all of: preprocessing the set of buoyant particles; adding a set of inputs to the reaction vessel; washing the set of buoyant particles; repeating one or more; and/or any other suitable process(es). 1. A system for processing a set of buoyant particles , the system comprising: a first inlet configured to receive a wash buffer;', 'a second inlet configured to receive a set of processing materials;', 'a third inlet configured to receive the set of buoyant particles; and', 'a first outlet;, 'a reaction vessel, the reaction vessel comprisinga stirring subsystem comprising a stir rod and an impeller, the impeller arranged within the reaction vessel; a set of hollow fibers, each of the set of hollow fibers having an inner diameter larger than a diameter of the set of buoyant particles;', 'a fourth inlet configured to receive the set of buoyant particles;', 'a second outlet;, 'a tangential flow filter comprisinga pump arranged downstream of the first outlet, wherein the pump comprises a diaphragm pump; and the first inlet with a wash buffer container, the wash buffer container comprising the wash buffer;', 'the second inlet with a processing material container, the processing material container comprising the set of processing materials;', 'the third inlet with the second outlet; and', 'the first outlet with the fourth inlet., 'a set of fluidic pathways configured to fluidly connect2. The system of claim 1 , wherein each of the set of buoyant particles has a particle diameter between 10 and 30 ...

DEVICE AND METHOD FOR THE DEPOSITION OF PARTICLES ON A TARGET

A device for deposition of particles on a target from a transparent slide having a film formed by a fluid containing suspended particles, by locally exciting the film using a laser, includes means for observing the local excitation region. The observation means comprise a sensor and a light source, the optical axes of which are substantially shared in a space between an optical splitter and that the film. The optical beam of the imaging system and the optical beam of the laser are coaxially arranged in the space between the controlled optical deflection means and the film. The device comprises a first focusing optical unit arranged between the controlled optical deflection means and the film. The device comprises a second image-combining optical unit positioned between the sensor and the splitter, the sensor being positioned in the focal plane of the second optical unit. 115.-. (canceled)16. A system for depositing particles on a target , comprising:a transparent slide for supporting a fluid film containing the particles in suspension;a laser configured to emit a laser beam onto a localized area of the fluid film;a deflection device configured to selectively deflect the laser beam onto selected localized areas of the fluid film;an imaging system configured to acquire images of the localized areas of the fluid film, the imaging system including a sensor and a light source, an optical axis of the sensor and an optical axis of the light source being at least substantially aligned with one another in a region between an optical splitter and the fluid film;a first focusing optical unit disposed between the deflection device and the fluid film; anda second image-combining optical unit disposed between the sensor of the imaging system and the optical splitter, the sensor being located in the focal plane of the second optical unit; andwherein the optical beam of an imaging system and the optical beam of the laser are coaxial in a region between the deflection device and the ...

CONTINUOUS FERMENTATION DEVICE

The present disclosure relates to a continuous fermentation device that can ferment objects continuously fed thereinto while conveying the objects. The present disclosure provides a continuous fermentation device comprising a plurality of fermentation modules, each including: a conveyor belt, which transports objects that are continuously fed thereinto to be fermented from one side to the opposite side thereof; agitators, which is located on the conveyor belt; and an air diffuser, which is located on one side of the conveyor belt, in which the continuously fed objects to be fermented are fermented while being passed through the fermentation modules. According to the present disclosure, feeding of raw materials to be fermented, fermenting the raw materials, and discharging of the fermented materials are continuously performed so that it is possible to significantly reduce the processing time, thereby reducing the operation time of the production process. Accordingly, it is possible to achieve an increase in competitiveness, such as improved productivity, reduction of investment cost, etc., in the development of a method of mass production through solid-state fermentation, thereby innovatively reducing the production cost of the product. 1100100. A continuous fermentation device comprising a plurality of fermentation modules , each of the plurality of fermentation modules comprising:{'b': '110', 'a conveyor belt , which transports objects to be fermented that are continuously fed thereinto from one side to the opposite side thereof;'}{'b': 200', '110, 'agitators , which are located on the conveyor belt ; and'}{'b': 300', '110, 'air diffusers , which are located on one side of the conveyor belt ,'}{'b': '100', 'wherein the continuously fed objects to be fermented are fermented while being passed through the fermentation modules and are continuously discharged.'}2100110100. The continuous fermentation device according to claim 1 , wherein the plurality of fermentation ...

High Pressure Bioreactor

The present invention relates generally to an integrated system, apparatus and method that allows for the continuous culturing of microorganisms under high pressure conditions and at a wide range of temperatures. More specifically, the system is configured to be gas tight and operate under aerobic or anaerobic conditions. The system is also configured to permit periodic sampling of the incubated organisms under such conditions with minimal physical/chemical disturbance inside the reactor and minimal impacts of shear forces on the collected biomass. 1. A method to allow for the continuous culturing of microorganisms under high pressure , the method comprising:a. providing a bioreactor capable of withstanding pressures of at least 150 MPa;b. pre-enriching media solution with dissolved gases in a reservoir;c. filling the bioreactor with growth media at high pressure and at the optimal temperature for growth;d. inoculating the bioreactor with culture;e. operating the bioreactor in the batch mode while the microbial community grows in density to a desired value;f. operating the bioreactor in a continuous mode by adding a continuous flow of media;g. increasing the pressure up to at least about 40 MPa; andh. monitoring the growth by sampling the reactor without affecting the hydrostatic pressure of the microbial community in bioreactor.2. The method of wherein the reactor is sampled using at least three valves arranged in series.3. The method of wherein the reservoir can withstand at least 60 psi of headspace partial pressure.4. The method of wherein the dissolved gases comprise one or more of H claim 1 , N claim 1 , COand O.5. The method of wherein the media solution is pre-heated prior to entering the bioreactor.6. The method of wherein the bioreactor is operated under aerobic or anaerobic conditions.7. The method of wherein sampling does not cause cell lysis.8. The method of wherein the pressure in the bioreactor is about 150 M Pa.9. A high pressure bioreactor for the ...

SUB-PASCAL UNIDIRECTIONAL FLOW VALVES

A valve includes a body including an inner bore extending between a first port and a second port, a seat, and one or more restrainers and a disk that is moveable between the seat and the one or more restrainers such that a first pressure that is less than 1 pascal and applied in a first direction causes the disk to move from a first position towards a second position to permit fluid communication between the first port and the second port. A metamaterial scaffold including a structure defining a lumen, at least a portion of an outer or non-lumen surface of the structure is coated with a plurality of biological cells, and wherein the structure is composed of a metamaterial. 1. A valve comprising:a body including an inner bore extending between a first port and a second port, a seat, and one or more restrainers; anda disk that is moveable between the seat and the one or more restrainers such that (i) a first pressure that is less than 1 pascal and applied in a first direction causes the disk to move from a first position towards a second position to permit fluid communication between the first port and the second port and (ii) a second pressure that is less than 1 pascal and applied in a second opposing direction causes the disk to move from the second position towards the first position to inhibit fluid communication between the first port and the second port.2. The valve of claim 1 , wherein the first pressure and the second pressure are less than 0.5 pascals.3. The valve of claim 1 , wherein the first pressure is between about 0.1 pascals and about 0.5 pascals.4. The valve of claim 1 , wherein the second pressure is between about 0.05 pascals and about 0.2 pascals.5. The valve of claim 1 , wherein the disk is moveable such that the first pressure causes the disk to move from the first position to the second position in less than 500 milliseconds.6. The valve of claim 1 , wherein the body is cylindrical and has a first diameter is that is 400 microns or less and a ...

DEVICE FOR DETERMINING ANTIMICROBIAL SUSCEPTIBILITY OF A MICROORGANISM

The present invention provides methods for determining the antimicrobial susceptibility of a microorganism in a clinical sample said method comprising removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units, isolating the microbial cells and transferring the cells into a suitable culture medium for microbial growth, and performing an AST assay using the isolated microbes, wherein the concentration of microbial cells in the microbial cells used to set up the AST assay is measured before the degree of microbial growth in the different growth conditions of the AST assay is measured. Devices for determining the antimicrobial susceptibility of a microorganism in a clinical sample are also provided. 126-. (canceled)27. A device for determining the antimicrobial susceptibility of a microorganism in a clinical sample , the device comprising:a test aliquot removal unit for removing a test aliquot from a clinical sample culture in a culture vessel;a transfer unit for transferring microbial cells isolated from the test aliquot into a culture medium suitable for microbial cell growth to thereby prepare a microbial culture preparation;an inoculation unit for inoculating a series of test microbial cultures for an antibiotic susceptibility test (AST);a measurement unit for determining concentration of microbial cells and for assessing the degree of microbial growth in the series of test microbial cultures; anda controller, wherein the controller is configured to:control the test aliquot removal unit to remove the test aliquot from the culture vessel when the culture in the culture vessel is less than 0.5 McFarland units;control the measurement unit to measure the concentration of microbial cells in the microbial culture preparation and/or the concentration of microbial cells in the test microbial cultures;control the device to adjust the concentration of microbial cells in said microbial culture preparation and/or said test microbial ...

METHOD OF PRINTING A TISSUE CONSTRUCT WITH EMBEDDED VASCULATURE

A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition. The fugitive ink is then removed to create vascular channels in the extracellular matrix composition, thereby forming an interpenetrating vascular network in a tissue construct. 124.-. (canceled)25. A three-dimensionally (3D) printed tissue construct with embedded vasculature , the printed tissue construct comprising:one or more tissue patterns, each tissue pattern comprising a plurality of viable cells of one or more predetermined cell types produced by depositing on a substrate one or more cell-laden filaments each comprising a plurality of viable cells:a network of interconnected vascular channels of various sizes interpenetrating the one or more tissue patterns, wherein the lamest channels of the network provide a single inlet and a single outlet for perfusion, while the smallest channels of the network reduce the characteristic diffusion distance between adjacent conduits; andan extracellular matrix composition at least partially surrounding the one or more tissue patterns and the ...

UNIVERSAL MICROFLUIDIC CULTURE SYSTEM TO ANALYZE AND CONTROL CELL DYNAMICS

Microfluidic devices, systems, and methods. 1. A microfluidic device comprising: an inlet;', 'an outlet;', 'a recess defining a chamber with a first portion and a second portion, the first portion having a first depth in the z direction, and the second portion having a second depth in the z direction that is greater than the first depth, a first port in the first portion of the chamber;', 'a first channel extending between the inlet and the first port;', 'a first valve (Valve 1) in fluid communication with the first port and configured to be selectively opened to permit fluid communication between the first port and the first channel;', 'a second port in the first portion of the chamber;', 'a second valve (Valve 2) in fluid communication with the second port and configured to be selectively opened to permit fluid communication with the second port;', 'a third port in the first portion of the chamber;', 'a third valve (Valve 3) in fluid communication with the third port and configured to be selectively opened to permit fluid communication with the third port;', 'a fourth port in the second portion of the chamber;', 'a fourth valve (Valve 4) in fluid communication with the fourth port and configured to be selectively opened to permit fluid communication with the fourth port;', the first channel between inlet and the first valve,', 'the second valve such that the second channel fluidly communicates with the second port when the second valve is open,', 'the outlet at a point farther from the fourth port than is the fourth valve, and', 'the third valve such that the second channel fluidly communicates with the third port when the second valve is open;, 'a second channel sequentially connecting, 'a fifth valve (Valve 5) disposed in the second channel and configured to be selectively closed to prevent fluid communication between the second valve and the outlet;', the first channel at the first valve such that the third channel fluidly communicates with the first port when ...

System and Workstation for the Design, Fabrication and Assembly of Bio-Material Constructs

A bioassembly system having a tissue/object modeling software component fully and seamlessly integrated with a robotic bioassembly workstation component for the computer-assisted design, fabrication and assembly of biological and non-biological constructs. The robotic bioassembly workstation includes a six-axis robot providing the capability for oblique-angle printing, printing by non-sequential planar layering, and printing on print substrates having variable surface topographies, enabling fabrication of more complex bio-constructs including tissues, organs and vascular trees. 123-. (canceled)24. A robotic biomaterial dispensing apparatus comprising:a robotic arm and a robotic arm end effector, the end effector configured to grip and secure a syringe barrel, wherein the robotic arm provides movement of a syringe along at least six axes;a material storage and dispensing system comprising a material storage unit comprising at least one syringe barrel holder, each barrel holder comprising multiple syringes, and the material storage unit further comprising at least one needle detection sensor for detecting needle size and tip deflection; andan adjustable print stage, wherein the robotic arm is configured to print biomaterial from the syringe from a plurality of directions onto a surface of the adjustable print stage based on an adjustable movement of the robotic arm and wherein the apparatus is contained within a housing.25. A method of fabricating a 3-D biological construct comprising utilizing the robotic biomaterial dispensing apparatus according to to direct-write dispense one or more biomaterials onto a print substrate by non-sequential planar layering.26. A method of fabricating a 3-D biological construct comprising utilizing the robotic biomaterial dispensing apparatus according to to direct-write dispense one or more biomaterials onto a 3-D print substrate claim 24 , wherein the robotic arm end effector positions the dispensing syringe at an angle normal to two ...

METHOD FOR SEPARATING MEGAKARYOCYTES AND PLATELETS, AND PLATELET SEPARATION KIT

An object of the present invention is to provide a method for efficiently separating megakaryocytes and platelets produced from the megakaryocytes, and a platelet separation kit for efficiently separating megakaryocytes and platelets produced from the megakaryocytes. According to the present invention, a method for separating megakaryocytes and platelets, including an incorporation step of incorporating magnetic fine particles into at least megakaryocytes; a culture step of culturing the megakaryocytes in a culture solution to produce platelets before and/or after the incorporation step; a magnetic field application step of applying a magnetic field to the culture solution after the incorporation step and the culture step; and a recovery step of recovering the culture solution after the magnetic field application step, is provided. 1. A method for separating megakaryocytes and platelets , the method comprising:an incorporation step of incorporating magnetic fine particles into at least megakaryocytes;a culture step of culturing the megakaryocytes in a culture solution to produce platelets before and/or after the incorporation step;a magnetic field application step of applying a magnetic field to the culture solution after the incorporation step and the culture step; anda recovery step of recovering the culture solution after the magnetic field application step.2. The method according to claim 1 , wherein the megakaryocytes are cultured in the culture solution to produce the platelets claim 1 , the magnetic fine particles are incorporated into the megakaryocytes and the platelets claim 1 , a magnetic field is applied to the culture solution claim 1 , and then the culture solution is recovered.3. The method according to claim 1 , wherein the magnetic fine particles are incorporated into the megakaryocytes claim 1 , the megakaryocytes are cultured in the culture solution to produce platelets claim 1 , a magnetic field is applied to the culture solution claim 1 , and ...

System and Method for Automatic Plant Tissue Sampling

An automatic plant tissue sampler and a method for operating the same are provided. The sampler can include a plant handler configured to transport a plurality of plants to an imager. The imager may be configured to image plants to identify a sampling location. The automatic plant tissue sampler also includes a sampler configured to remove a tissue sample from the sampling location of plants, and a collection vessel configured to receive the tissue samples. The automatic plant tissue sampler may transport a plurality of plants to an imager and images the plurality of plants to identify a sampling location. The automatic plant tissue sampler can remove a tissue sample from the sampling location of the plurality of plants and store the tissue samples in a collection vessel for testing. 130-. (canceled)31. An automatic plant sampling system for automatically sampling plant tissue of an individual plant selected from a group of one or more plants collectively conveyed to the automatic plant sampling system , the automatic plant sampling system comprising:a frame assembly having a first axis, a second axis orthogonal to the first axis, and a third axis orthogonal to the first axis and the second axis;a plant handling system coupled to the frame assembly so that a portion of the plant handling system moves relative to the frame assembly, the plant handling system configured to select the individual plant from the one or more plants, transport the individual plant to a sampling system for sampling, and transport the individual plant from the sampling system to a post sampling location;the sampling system coupled to the frame assembly proximate to the plant handling system, the sampling system configured to determine a suitable sampling location of the individual plant, sample a portion of plant tissue from the sampling location to create a tissue sample, and transport the tissue sample to a storage system;the storage system coupled to the frame assembly proximate to the ...

Method for cultivating a microorganism of interest and associated facility

A method for cultivating at least one microorganism of interest, by heterotrophism or mixotrophism, in an aqueous culture medium, contaminating microorganisms developing naturally in the culture medium. A portion of the culture medium with the microorganism of interest and the contaminating microorganisms is sampled. The microorganism of interest and the contaminating microorganisms in the portion of culture medium is physically separated. The contaminating microorganisms thus separated is lysed to produce a lysate. The lysate is reintroduced into the culture medium.

STEM CELL MANUFACTURING SYSTEM, STEM CELL INFORMATION MANAGEMENT SYSTEM, CELL TRANSPORT APPARATUS, AND STEM CELL FROZEN STORAGE APPARATUS

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state. 1. A stem cell manufacturing system for manufacturing stem cells from somatic cells , the system comprising:one or more closed production device(s) configured to produce stem cells from somatic cells;one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells;one or more cryopreservation device(s) configured to cryopreserve the produced stem cells;a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state;a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; anda third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.2. The stem cell manufacturing system according to claim 1 , further comprising an entry memory device ...

DEVICES, SYSTEMS AND METHODS FOR AUTOMATED TRANSFER OF A SAMPLE

A coupling device configured to form a sample access assembly is provided. The sample access assembly is configured to house a sample. The coupling device includes a heating component and a separating component. Further, the separating component is configured to separate portions of first and second containers that form first and second compartments of the sample access assembly. Moreover, the heating component is configured to heat at least a portion of the sample. 1. A coupling device configured to form a sample access assembly using first and second containers , wherein the sample access assembly comprises a first compartment and a second compartment , and wherein the sample access assembly is configured to house a sample , the coupling device comprising:a heating component configured to heat at least a portion of the sample; anda separating component configured to separate at least a portion of the first container and at least a portion of the second container from remaining portions of the first and second containers to form the first and second compartments of the sample access assembly,wherein the coupling device is configured to transfer at least a portion of the sample, and wherein the coupling device is configured to maintain a sterile environment for the sample at least during coupling of the first and second compartments.2. The coupling device of claim 1 , wherein the first and second compartments are respective portions of the first and second containers.3. The coupling device of claim 2 , further comprising:a first holder unit configured to receive at least a portion of the first container, wherein the first container is configured to receive the sample; anda second holder unit operatively coupled to the first holder unit, wherein the second holder unit is configured to receive at least a portion of the second container,wherein the first and second holder units comprise one or more degrees of freedom, and wherein the first and second holder units are ...

PROCESSING BIOMASS

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material. 1. A system for producing a treated biomass , the system comprising:(i) a module configured to determine the lignin content of a lignocellulosic biomass feedstock;(ii) an electron beam irradiating system for irradiating the feedstock, the radiation parameters of the irradiating system being adjustable based on the determined lignin content;(iii) a vessel configured to receive irradiated feedstock and a fluid;(iv) a delivery system configured to deliver the feedstock, an enzyme, and the fluid to the vessel; and(v) a recovery system configured to recover a product from the vessel.2. The system of further comprising a device for monitoring an output parameter of the irradiating system or the recovery system.3. The system of wherein the monitoring device is configured to provide data to the irradiating system based upon which the radiation parameters can be adjusted.4. The system of wherein the recovery system is configured to recover lignin from the vessel.5. The system of wherein the delivery system is configured to deliver a microorganism to the vessel.6. The system of further comprising a module configured to mechanically treat the feedstock. This application is a continuation application of U.S. application Ser. No. 14/494,815, filed Sep. 24, 2014, which is a continuation application of U.S. application Ser. No. 13/786,920, filed Mar. 6, 2013, now U.S. Pat. No. 8,877,467, granted on Nov. 4, 2014, which is a continuation application of U.S. application Ser. No. 12/704,519, filed Feb. 11, 2010, now U.S. Pat. No. 8,415,122, granted on Apr. 9, 2013, which claims priority to U.S. Provisional Application ...

Method for selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays

The present invention relates to a method for selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays. More particularly, conducting polymers or metal oxides having exothermic characteristics upon irradiation of near infrared light is used as a cell culture scaffold, thus selectively attaching/detaching cells without an enzyme treatment. The scaffold has an effect of promoting proliferation or differentiation of stem cells, and therefore, can be used as a stem cell culture scaffold. The scaffold enables cell attachment/detachment without temporal or spatial restrictions, thus enabling cell patternization.

Funnel For Localizing Biological Cell Placement And Arrangement

The present disclosure relates to a funnel apparatus for channeling cells onto a plurality of distinct, closely spaced regions of a seeding surface. The funnel apparatus has a body portion having an upper surface and a lower surface. The body portion forms a plurality of flow paths, at least one of which is shaped to have a decreasing cross-sectional area from the upper surface to the lower surface. The flow paths are formed at the lower surface to enable cells deposited into the flow paths at the upper surface of the funnel apparatus to be channeled into a plurality of distinct, closely spaced regions on the seeding surface positioned adjacent the lower surface. 1. A funnel apparatus for channelling cells onto a plurality of distinct , closely spaced regions of a seeding surface , the funnel apparatus comprising:a body portion having an upper surface and a lower surface;the body portion forming a plurality of flow paths, at least one of which is shaped to have a decreasing cross-sectional area from the upper surface to the lower surface; andwherein the flow paths are formed at the lower surface to enable cells deposited into the flow paths at the upper surface of the funnel apparatus to be channeled into a plurality of distinct, closely spaced regions on the seeding surface positioned adjacent the lower surface.2. The funnel apparatus of claim 1 , wherein each of the flow paths has a decreasing cross sectional area from the upper surface to the lower surface of the body portion.3. The funnel apparatus of claim 1 , wherein the plurality of flow paths are formed by:at least a plurality of radially extending wall portions; andat least one circumferential wall portion that intersects the plurality of radially extending wall portions.4. The funnel apparatus of claim 3 , wherein at least one of the plurality of flow paths has an arcuate shape; andwherein at least one of the plurality of flow paths has a pie shape.5. The funnel apparatus of claim 4 , wherein the plurality ...

System for delivery of microbial inoculants and related materials and methods

Described herein are systems, methods and kits for culturing and applying fresh microbial inoculants in the field. The system, methods and kits are easy to use, reliable, sealed to prevent contamination, and can be stored on location, for use on demand by those unskilled in the microbiological arts. Particular embodiments described herein may be used to increase agricultural crop yields.

Cell Expansion Vessel Systems and Methods

A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port. 1. A cell culture vessel for expansion of a cell density , comprising:a bottom plate comprising a grid surface configured to provide support and gas exchange;a vessel body coupled to the bottom plate, wherein the vessel body comprises a rigid concave structure;a cavity formed by the vessel body and the bottom plate;a gas-permeable, liquid impermeable membrane disposed above the bottom plate within the cavity;a feeding port disposed through a surface of the vessel body, wherein the feeding port is configured to permit addition of cell culture medium into the cavity; anda drain port disposed ...

System and process for recovering algal oil

Herein disclosed is a method of processing a medium containing algae microorganisms to produce algal oil and by-products, comprising providing the medium containing algae microorganisms; passing the medium through a rotor-stator high shear device; disintegrating cell walls of and intracellular organelles in the algae microorganisms to release algal oil and by-products; and removing the algae medium from an outlet of the high shear device. In an embodiment, disintegration is enhanced by a penetrating gas capable of permeating the cell wall. In an embodiment, enhancement is accomplished by super-saturation of the penetrating gas in the medium or increased gas pressure in a vessel. In an embodiment, the penetrating gas is different from the gas produced by the cell during respiration. A suitable system is also discussed in this disclosure.

Microorganism growth and products recovery

Herein disclosed is a method of generating products from microorganisms, comprising super-saturating a liquid medium with a gas consumable by the microorganisms in a high shear device operating at a shear rate of greater than 1,000,000 s−1 to produce a gas-super-saturated (GSS) medium, wherein the GSS medium maintains a GSS level for at least 10 minutes; feeding the GSS medium to microorganisms; allowing the microorganisms to grow by consuming the gas and generate products via photosynthesis or chemosynthesis; and recovering the products. In an embodiment, the microorganisms are genetically modified. In an embodiment, the microorganisms include bacteria, protozoa, algae, or fungi, or a combination thereof. In an embodiment, the gas consumable by the microorganisms is selected from the group consisting of carbon dioxide, nitrogen, air, oxygen, methane, and combinations thereof. A suitable system is also discussed in this disclosure.

HIGH-THROUGHPUT DRUG AND GENETIC ASSAYS FOR CELLULAR TRANSFORMATION

A method for detecting oncogenic growth and viability, and/or degree of cellular transformation and/or identifying an agent that inhibits cellular transformation is disclosed. The method including: providing a cellular sample, such as a sample of cells obtained from a subject or a cell line; culturing the cellular sample in low attachment conditions; and detecting growth and7or cell viability of the sample, wherein increased growth relative and/or viability relative to a control or control level indicative of basal growth and/or viability indicates cellular transformation. In some embodiments, the method includes introducing a n expression vector into cells of the cellular sample, wherein the expression vector comprises a gene product expression sequence being tested for transformation ability. In some embodiments the cellular sample is contacted with a test agent and growth and/or cell viability of the sample is determined to determine if the agent inhibits transformation. 1. A method for identifying an agent that inhibits cellular growth and/or viability of tumorigenic or transformed cells , the method comprising:providing a cellular sample of transformed cells and/or cells obtained from the tumor of a subject;culturing the cellular sample in low attachment conditions;contacting or incubating the cellular sample with the agent; anddetecting growth and/or cell viability of the sample, wherein decreased growth relative and/or viability relative to a control not contacted with the agent or control level indicative of basal growth and/or viability identifies the agent as one that inhibits cellular growth and/or viability of tumorigenic or transformed cells.2. (canceled)3. The method of claim 1 , wherein the cells are transformed with an expression vector encoding one or more genetic perturbations; optionally claim 1 ,wherein the expression vector further comprises a nucleic acid barcode that identifies the one or more genetic perturbations; and/orwherein the genetic ...

CELL CULTURE INSTRUMENT AND CELL PROCESSING METHOD

A cell culture instrument configured to detach cells at a desired position and a cell processing method using the cell culture instrument. The cell culture instrument includes: a substrate; and a photoreactive layer having a photosolubility and a photothermal convertibility, wherein the photoreactive layer is laminated on the substrate, and the photoreactive layer includes a polymer having a photosolubility and a photothermal convertibility. 1. A cell culture instrument , comprising:a substrate; anda photoreactive layer having a photosolubility and a photothermal convertibility, whereinthe photoreactive layer is laminated on the substrate, andthe photoreactive layer comprises a polymer having a photosolubility and a photothermal convertibility.2. The cell culture instrument according to claim 1 , whereinthe photoreactive layer comprises a photosoluble layer having a photosolubility and a photothermal convertible layer having a photothermal convertibility,the photosoluble layer comprises a photosoluble polymer, andthe photothermal convertible layer comprises a photothermal convertible polymer.6. The cell culture instrument according to claim 2 , whereinthe photothermal convertible polymer has a main chain and a side chain, andthe side chain has a chromophore having a predetermined absorbance at a wavelength of 350 nm or more.7. The cell culture instrument according to claim 6 , wherein the chromophore has an azobenzene skeleton.10. The cell culture instrument according to claim 1 , comprising:a connection layer connecting the photoreactive layer and the substrate, whereinthe connection layer is laminated on the substrate, andthe photoreactive layer is laminated on the connection layer.11. The cell culture instrument according to claim 1 , wherein cells are cultured on the photoreactive layer.12. A cell processing method claim 1 , comprising:{'claim-ref': {'@idref': 'CLM-00001', '#text': 'claim 1'}, '#text': 'culturing cells in the cell culture instrument according to ...

ANALYSIS AND SORTING OF MOTILE CELLS

A method for sorting motile cells includes introducing an initial population of motile cells into an inlet port of a microfluidic channel, the initial population of motile cells having a first average motility; incubating the population of motile cells in the microfluidic channel; and collecting a sorted population of motile cells at an outlet port of the microfluidic channel. The sorted population of motile cells has a second average motility higher than the first average motility. 1. A method for sorting motile cells , comprising:introducing an initial population of motile cells into an inlet port of a microfluidic channel, the initial population of motile cells having a first average motility;incubating the population of motile cells in the microfluidic channel; andcollecting a sorted population of motile cells at an outlet port of the microfluidic channel, the sorted population of motile cells having a second average motility higher than the first average motility.2. The method of claim 1 , further comprising orienting the microfluidic channel horizontally or vertically.3. The method of claim 1 , wherein incubating the population of motile cells includes incubating in the absence of flowing media.4. The method of claim 1 , wherein incubating the population of motile cells includes incubating the population of motile cells for a time sufficient to allow a portion of the initial population of motile cells to move along the microfluidic channel claim 1 , e.g. claim 1 , for about 20-60 minutes claim 1 , or about 30 minutes.5. The method of claim 1 , wherein the height of the microfluidic channel is less than about 20 times a dimension of the motile cells claim 1 , e.g. claim 1 , about 3 to 10 times the dimension of the motile cells.6. The method of claim 1 , further comprising determining the second average motility claim 1 , including:obtaining a plurality of images, e.g., shadow images, of a collectable population of motile cells in the vicinity of the outlet port ...

Movement sequence analysis utilizing printed circuits

A tool for movement sequence analysis utilizing printed circuits. The tool determines a workflow sequence based, at least in part, on one or more printed circuits within a printed organic component. The tool activates the workflow sequence utilizing applied external stimuli on the one or more printed circuits. Responsive to activating the workflow sequence, the tool analyzes a signal response from the one or more printed circuits. The tool generates one or more printing recommendations based, at least in part, on the signal response analysis.

Instruments, modules, and methods for improved detection of edited sequences in live cells

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

METHOD AND APPARATUS FOR PRINTING BIOLOGICAL TISSUES AND ORGANS

The invention relates to medicine and biology, particularly to the means for artificial manufacturing of biological tissues and organs, and can be used in biotechnology, bioengineering, tissue engineering, regenerative medicine, and in the 3D-printing of biological tissues and organs. 1. The layer by layer method of biological tissue and organ printing using bioink or a gel-forming composition polymerizing when exposed to UV light and involving the following stages of each individual layer formation:a. acquiring data on the object's layer selected for printing; 'the bioink printing module is separated from the gel-forming-composition printing module in such a way so as to prevent UV radiation from reaching the bioink printing module, the radiation from the UV module being directed predominantly parallel to the platform for printing, in such a way so as to prevent UV radiation from reaching the biological tissues and/or organs being printed, and the amount of gel-forming composition is calculated so as to form the required hydrogel layer upon completion of the polymerization process;', 'b. deposing on the final surface a gel-forming composition polymerizing when exposed to UV light and which in the process of the deposition, i.e. after being released from the nozzle and before deposition of the biological tissue or organ being printed, is exposed to UV radiation;'}c. applying bioink on the layer formed with the gel-forming composition at the previous stage (b) in accordance with the data acquired at stage (a);d. wait on hydrogel polymerization completion.2. The method of claim 1 , characterized in that spheroids are used as bioink.3. The method of claim 2 , characterized in that the ratio between the spheroid diameter and the thickness of the polymerized hydrogel layer selected so that the thickness of the latter is less than the spheroid diameter.4. The method of claim 3 , characterized in that the spheroid diameter is in the range between 100 μm and 2.108 mm claim ...

Microfluidic System for Reproducing Functional Units of Tissues and Organs In Vitro

A microfluidic system including a number of microfluidic devices having a first perfusion path and a second separate perfusion path; the microfluidic devices each also having a chamber containing a matrix, where the matrix surrounds at least one void whose lumen is in fluidic connection exclusively with the first perfusion path, where the at least one void is populated with at least one cell type in such way that the cells are in direct contact with the matrix; where the matrix is in fluidic connection exclusively with the second separate perfusion path. The microfluidic devices are integrated onto a platform; and each of the microfluidic devices mimics at least a partial organ module. 1. A microfluidic system for reproducing functional units of tissues and organs in vitro comprising: a plurality of microfluidic devices having at least a first perfusion path and a second separate perfusion path; the plurality of microfluidic devices each also having a chamber containing a matrix , where the matrix surrounds at least one void whose lumen is in fluidic connection exclusively with the first perfusion path , where the at least one void is populated with at least one cell type in such way that the cells are in direct contact with the matrix; where the matrix is in fluidic connection exclusively with the second separate perfusion path; wherein the plurality of microfluidic devices are integrated onto a platform; and wherein each of the plurality of microfluidic devices mimics at least a partial organ module.2. The system of wherein the organ modules are selected from the group consisting of intestine claim 1 , liver claim 1 , kidney claim 1 , and blood-brain barrier modules.3. The system of wherein the plurality of microfluidic devices are connected to form a complex system with each microfluidic device representing a different organ type.4. The system of wherein a central claim 2 , two-compartment liver module is connected to a kidney module claim 2 , an intestine module ...

INSTRUMENTS, MODULES, AND METHODS FOR IMPROVED DETECTION OF EDITED SEQUENCES IN LIVE CELLS

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited. 1. A method for enriching edited cells during nucleic acid-guided nuclease editing comprising:transforming cells with one or more vectors comprising a promoter driving expression of a CRISPR nuclease, an inducible promoter driving transcription of a guide nucleic acid covalently-linked to a DNA donor sequence and wherein each of the one or more vectors comprises a gene for a selectable marker;diluting the transformed cells to a cell concentration to substantially singulate the transformed cells on a first substrate;growing the cells to form colonies on the first substrate;initiating editing in the cells by inducing the inducible promoter driving transcription of the guide nucleic acid;growing the induced cells into terminal-sized colonies;making a replica of the first substrate forming a second substrate;growing and inducing cells on the second substrate under conditions that do not allow genome repair;comparing cell growth on the first and second substrates; andselecting cells that grow on the first substrate but do not grow on the second substrate.2. The method of claim 1 , wherein the promoter driving transcription the guide nucleic acid and donor DNA is a pL promoter.3. The method of claim 1 , wherein the promoter driving expression of the CRISPR nuclease is an inducible promoter.4. The method of claim 3 , wherein the inducible promoter driving expression of each of the guide nucleic acid and the CRISPR nuclease is the same inducible promoter.5. The method of claim 4 , wherein the inducible promoter driving expression of the guide nucleic acid and driving transcription of the guide nucleic ...

SYSTEMS AND METHODS FOR SEEDING CELL CULTURES IN A MICROFLUIDIC DEVICE

This disclosure provides systems and methods for seeding cell cultures in a microfluidic device. The systems and methods of this disclosure can enable flow of a cell solution from one side of a scaffold, such as a porous substrate or membrane, to the other side of the scaffold. Flow of the liquid can pass through the scaffold while the cells themselves do not, resulting in the cells driven to the surface of the scaffold for consequent attachment. A microfluidic device can include a microfluidic feature structured to create a seal between a cell seeding tool and an inlet to a microchannel of the microfluidic device. This can enable a pressure-driven flow to push fluid down the channel and through pores of the membrane. In contrast, traditional gravity fed seeding of cells may not create enough pressure to drive fluid through the pores of the scaffold. 1. A microfluidic device , comprising:a first channel having a first inlet port and a first outlet port, the first channel configured to receive a fluid sample comprising cells;a second channel coupled to the first channel via an overlapping region, the second channel having a second inlet port and a second outlet port;an overlapping region between the first channel and the second channel, configured to trap the cells in the fluid sample as the fluid sample flows through the first channel.2. The microfluidic device of claim 1 , wherein the overlapping region forms a bottom portion of the first channel and forms a top portion of the second channel.3. The microfluidic device of claim 1 , wherein at least one of the first inlet port claim 1 , first outlet port claim 1 , second inlet port claim 1 , or second outlet port is coupled to a well of a well plate.4. The microfluidic device of claim 1 , wherein the overlapping region comprises a semipermeable membrane configured to allow the fluid sample to flow from the first channel to the second channel while trapping the cells in the fluid sample on a surface of the ...

Process for creating reference data for predicting concentrations of quality attributes

A process and system for efficiently producing reference data that can be fed into a predictive model for predicting quality attribute concentrations in cell culture processes. A perfusion bioreactor is operated at pseudo-steady-state conditions and one or more attribute influencing parameters are manipulated and changed over time. As the one or more attribute influencing parameters are manipulated, one or more quality attributes are monitored and measured. In one embodiment, multiple quality attributes are monitored and measured in parallel. The quality attribute information is recorded in conjunction with the changes in the attribute influencing parameters. This information is then fed to the predictive model for propagating cell cultures in commercial processes and maintaining the cell cultures within desired preset limits.

Devices, systems, and methods for the fabrication of tissue

Described herein are bioprinters comprising: one or more printer heads, wherein a printer head comprises a means for receiving and holding at least one cartridge, and wherein said cartridge comprises contents selected from one or more of: bio-ink and support material; a means for calibrating the position of at least one cartridge; and a means for dispensing the contents of at least one cartridge. Further described herein are methods for fabricating a tissue construct, comprising: a computer module receiving input of a visual representation of a desired tissue construct; a computer module generating a series of commands, wherein the commands are based on the visual representation and are readable by a bioprinter; a computer module providing the series of commands to a bioprinter; and the bioprinter depositing bio-ink and support material according to the commands to form a construct with a defined geometry.

DEVICE FOR AUTOMATED SEEDING OF CULTURE MEDIUM

The invention relates to a device () for automated inoculation of a culture medium, the device being of the type comprising: 1. A device for automated inoculation of a culture medium , the device comprising:a clamp suitable for gripping the inoculation tool;a clamp-holder;a support base carrying the assembly formed by the clamp and the clamp-holder; anda support structure for supporting the support base and for enabling the support base to be moved along three axes;wherein the assembly formed by the clamp and the clamp-holder is an assembly mounted to move in pivoting about an axis (CC′) in such a manner that it is tillable relative to the support base, in that the clamp is fitted with pivot branches mounted to pivot between two positions, one being an open position and the other being a closed position, pivoting being driven by drive means preferably acting on return means, and in that the clamp is a rotary clamp mounted to revolve about an axis (BB′) that is transverse and preferably orthogonal to the pivot axis (AA′) of the branches of the clamp.2. A device according to claim 1 , wherein the axis of rotation (BB′) about which the clamp revolves and the tilt axis (CC′) of the clamp and clamp-holder assembly are substantially mutually orthogonal.3. A device according to claim 1 , wherein the drive means for driving the branches of the clamp pivotally comprise at least one “linear” cam arranged between the branches of the clamp claim 1 , and motor means for driving the cam axially along an axis that coincides with the axis of rotation (BB′) of the clamp following a path during which the cam is suitable for pressing against said branches of the clamp claim 1 , at least from time to time.4. A device according to claim 3 , the motor means for driving the cam axially back and forth comprise a nut-and-leadscrew assembly claim 3 , in which the screw claim 3 , of axis that coincides with the axis of rotation (BB′) of the clamp claim 3 , holds the cam and in which the nut ...

CASSETTE FOR STERILITY TESTING

The invention provides a device for growing cells—referred to as a cassette. The cell culturing device includes a housing that contains a lid having an optically clear window; a fluid distribution channel; a sample injection port fluidically connected to the fluid distribution channel; a base housing a porous media pad; and a media injection port fluidically connected to the media pad. The lid mates to the base to form a sterile seal; the fluid distribution channel is disposed over the media pad, which is viewable through the optical window; and sample fluid introduced into the fluid distribution channel is distributed evenly to the media pad, e.g., via a plurality of channels. The invention also provides kits that include cassettes of the invention and a tube set. 131.-. (canceled)32. A cell culturing device comprising a membrane , wherein the device allows filtration of a sample through the membrane , and the membrane is viewable for imaging , wherein the device allows for the addition of nutrient media to support cell growth , and wherein the device is sealed to prevent contamination.33. The device of claim 32 , comprising a lid having an optically clear window through which the membrane is viewable.34. The device of claim 32 , comprising a sample inlet port and a drainage port claim 32 , wherein a sample introduced through the sample inlet port flows through the membrane to the drainage port.35. The device of claim 32 , comprising a porous media pad to house the nutrient media.36. The device of claim 32 , further comprising an oxygen scavenger sufficient to render the interior of the device anaerobic.37. The device of claim 36 , further comprising an actuator for the oxygen scavenger.38. The device of claim 32 , further comprising a pressure-relief valve.39. The device of claim 32 , further comprising a vent port for venting the interior of the device as liquids are introduced.40. The device of claim 39 , wherein the vent port is self-sealing. This application ...

Method and device suitable for growing algae

Disclosed are methods and devices suitable for growing algae.

CELL MAINTAINER FOR AUTOLOGOUS CELL THERAPY PRODUCTION

In some aspects, the invention relates to automated cell culture incubators and their methods of use. In one aspect, the disclosure provides cell culture incubators having an airlock chamber, a storage chamber and/or an internal chamber. In some aspects, the disclosure provides methods for producing autologous mammalian cell cultures. 150-. (canceled)51. A cell culture incubator comprising:first and second internal chambers, each having a controlled temperature and gas mixture therein appropriate for the growth of a type of cell for incubation of said type of cell in one or more cell culture vessels in the internal chambers, wherein the first internal chamber comprises a storage location for storing a plurality of cell culture vessels and wherein the second internal chamber comprises at least one imager;an automated transfer device for moving at least one of the plurality of cell culture vessels between the storage location in the first internal chamber and the at least one imager in the second internal chamber, wherein the transfer device includes at least one robotic element in at least one of the chambers and wherein the storage location is configured to automatically move the at least one of the plurality of cell culture vessels into a position to be transferred by the automated transfer device;at least one processor configured to control the movement of the at least one of the plurality of cell culture vessels in the storage location and the transfer of the at least one of the plurality of cell culture vessels to and from the storage location; andsensors for sensing the position of each cell culture vessel in the chambers of the incubator and communicating with the at least one processor to enable the at least one processor to determine the location of each cell culture vessel for desired transfer of each cell culture vessel between the storage location and the at least one imager.52. The cell culture incubator according to claim 51 , wherein the storage ...

Generating induced pluripotent stem cells

Procedures for generating induced pluripotent stem cells include applying stress to a somatic cell to produce strain that induces the somatic cell to become a pluripotent stem cell without a need for transfection. Generating induced pluripotent stem cells can include forming a droplet that surrounds a somatic cell; and applying mechanical stress to the somatic cell to produce mechanical strain that induces the somatic cell to become a pluripotent stem cell without transfection, where forming the droplet includes thermal inkjet printing. This can also include printing the pluripotent stem cell on a substrate and incubating the pluripotent stem cell. In that case, forming an aggregate composing the pluripotent stem cell can include, after printing and before incubating, positioning the substrate above the pluripotent stem cell whereby gravity exerts a force on the pluripotent stem cell away from the substrate.

METHODS OF SELECTIVE CELL ATTACHMENT/DETACHMENT, CELL PATTERNIZATION AND CELL HARVESTING BY MEANS OF NEAR INFRARED RAYS

The present invention relates to a method for selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays. More particularly, conducting polymers or metal oxides having exothermic characteristics upon irradiation of near infrared light is used as a cell culture scaffold, thus selectively attaching/detaching cells without an enzyme treatment. The scaffold has an effect of promoting proliferation or differentiation of stem cells, and therefore, can be used as a stem cell culture scaffold. The scaffold enables cell attachment/detachment without temporal or spatial restrictions, thus enabling cell patternization. 2. The method of detaching cultured cells according to claim 1 , wherein the cell culture region is formed of any one of polycarbonate claim 1 , polypropylene claim 1 , polyethylene claim 1 , polystyrene claim 1 , polyurethane claim 1 , polyethylene terephthalate claim 1 , polyester claim 1 , polyimide claim 1 , polyethylene glycol claim 1 , polydimethylsiloxane claim 1 , and a copolymer or composite thereof; Nylon; paper; cotton; and glass.3. The method of detaching cultured cells according to claim 1 , wherein the polymer or copolymer has a weight average molecular weight of 1 claim 1 ,000 to 1 claim 1 ,000 claim 1 ,000 Da.4. The method of detaching cultured cells according to claim 1 , wherein the polymer or copolymer film has a thickness of 10 nm to 1 mm.5. The method of detaching cultured cells according to claim 1 , wherein the cells include adult stem cells derived from breasts claim 1 , bone marrow claim 1 , cord blood claim 1 , blood claim 1 , liver claim 1 , skin claim 1 , gastrointestine claim 1 , placenta claim 1 , or womb. This application is a continuation-in-part of co-pending U.S. application Ser. No. 14/342,451, filed Mar. 3, 2014.1. Field of the InventionThe present invention relates to methods for selectively detaching, patterning, and harvesting cells using near-infrared capable of being used ...

Specimen processing system and method for processing specimen

A specimen processing system includes a culture vessel, a spatula, a robot, and a robot controller. The culture vessel is open on a top surface and includes a circular culture surface on which cells are culturable. The spatula includes a blade to remove the cells off the culture surface. The robot includes a hand to hold the culture vessel and/or the spatula. The robot controller includes an operation controller to control the robot to: move the spatula relative to the culture vessel with a first end of the blade on a circumference of the culture surface to remove first cells that are nearer the circumference of the culture surface; and move the spatula relative to the culture vessel to remove second cells that are inner than the first cells on the culture surface.

INSTRUMENTS, MODULES, AND METHODS FOR IMPROVED DETECTION OF EDITED SEQUENCES IN LIVE CELLS

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited. 1. A method of growing cells in a solid wall isolation , incubation and normalization (SWIIN) module comprising: [ an upper surface and a lower surface and a first and second end,', 'an upper portion of a serpentine channel defined by raised areas on the lower surface of the retentate member, wherein the upper portion of the serpentine channel traverses the lower surface of the retentate member for about 50% to about 90% of the length and width of the lower surface of the retentate member;', 'at least retentate one port fluidically connected to the upper portion of the serpentine channel; and', 'a reservoir cover at the first end of the retentate member;, 'a retentate member comprising;'}, an upper surface and a lower surface and a first and second end,', 'a lower portion of a serpentine channel defined by raised areas on the upper surface of the permeate member, wherein the lower portion of the serpentine channel traverses the upper surface of the permeate member for about 50% to about 90% of the length and width of the upper surface of the permeate member, and wherein the lower portion of the serpentine channel is configured to mate with the upper portion of the serpentine channel to form a mated serpentine channel;', 'at least one permeate port fluidically connected to the lower portion of the serpentine channel;, 'a permeate member disposed under the retentate member comprising, 'a first and second reservoir at the first end of the permeate member, wherein the first reservoir is fluidically connected to the at least one retentate port in the retentate member and the second reservoir is ...

Cell structure connection method and connection support device

The connection support device includes a rod-shaped member insertable into respective hollow portions of two or more cell structures, the rod-shaped member being inserted into the hollow portions, the rod-shaped member including a circular cross section having an outer diameter capable of adhering to inner surfaces of the cell structures when the rod-shaped member contracts after maturing, and a total length longer than a sum of respective lengths of the two or more cell structures, and two presser devices including clamp portions capable of being fixed to the rod-shaped member by clamping and fitting to the rod-shaped member, the rod-shaped member being made of a material with oxygen permeability.

DEVICE AND METHOD FOR AUTOMATIC PROCESSING OF CULTURE PLATES FOR MICROBIOLOGICAL SAMPLES

An automatic processing device of culture plates () including a support frame (); a slide () for removably housing a culture plate () and movably mounted on the support frame () selectively displaceable between first loading position, a plurality of image-acquiring positions, and a first unloading position; a camera () of a linear type, provided with telecentric optic () and a trilinear sensor, and arranged to acquire a multiplicity of linear images of corresponding linear portions of an upper surface of the culture plate (), during the displacing of the slide (); a first lighting device () orientated such as to illuminate the linear portions of an upper surface of the culture plate (); an advancing device () of the slide () configured such as to enable obtaining a constant and substantially vibration-free advancing speed of the slide () in the image-acquiring zone; and an electronic control device () of a functioning of the camera (), of the lighting device and of the advancing device (). 110-. (canceled)1121. An automatic processing device of culture plates () for microbiological samples , wherein the processing device () comprises:{'b': '3', 'a support frame ();'}{'b': 4', '5', '2', '4', '3', '2', '4', '2', '4, 'a slide () or shuttle provided with a seating () configured for removably housing, in a horizontal position, a culture plate () for microbiological samples, the slide () being movably mounted on the support frame () so as to be selectively displaceable between a first loading position of the culture plate () on the slide (), a plurality of image-acquiring positions, and a first unloading position of the culture plate () from the slide ();'}{'b': 6', '7', '8', '2', '4', '4', '2, 'a camera () of a linear type, provided with an optic () of a telecentric type and a trilinear sensor, and arranged according to a vertical axis () such as to acquire, at an image-acquiring zone, a multiplicity of linear images of corresponding linear portions of an upper surface ...

DEVICE AND METHOD FOR DISPENSING A SUSPENSION OF MICROORGANISMS

A device including an applicator capable of dispensing a suspension of microorganisms on a culture medium and to the use of the device for dispensing the suspension of microorganisms. 1. A device comprising an applicator capable of dispensing a suspension of microorganisms on a culture medium which is itself deposited on a support , wherein its position of contact with the suspension of microorganisms , the applicator comprises at least two surfaces of contact with the suspension of microorganisms , each surface defines a plane , the at least two planes being parallel to one another and having a distance between them of a height h , the at least two parallel planes face the interface between said suspension of microorganisms and the culture medium and are also arranged parallel to said interface.2. The device as claimed in claim 1 , wherein h is equal to h-h claim 1 , hbeing the distance between the first surface of contact and the interface claim 1 , hbeing the distance between the second surface of contact and the interface claim 1 , and that the h/hratio is greater than 1 and less than or equal to 100.3. The device as claimed in claim 2 , wherein Scorresponds to the area of the first surface of contact of the applicator and Scorresponds to the area of the second surface of contact of the applicator claim 2 , and that the (h×S)/(h×S) ratio is greater than 1 and less than or equal to 1000.4. The device as claimed in claim 3 , wherein viewed perpendicular to the parallel planes claim 3 , Sand Sdo not overlap claim 3 , likewise the surfaces of contact.5. The device as claimed in claim 2 , wherein h/his equal to 3.6. The device as claimed in claim 1 , wherein it comprises at least one means for leaving a space between the surfaces of contact of the applicator and the support or the interface.7. The device as claimed in claim 6 , wherein the means for leaving a space and the applicator are interdependent.8. The device as claimed in claim 6 , wherein the means for ...

DISPOSABLE KIT FOR BIOPROCESSING

A system for bioprocessing includes a tray having plurality of sidewalls and a bottom surface defining an interior compartment, and a generally open top, and an opening formed in the bottom surface of the tray, the opening having a perimeter. The perimeter of the opening is shaped and/or dimensioned such that a bioreactor vessel can be positioned above the opening and supported by the bottom surface of the tray while a portion of the bioreactor vessel is accessible through the opening in the bottom surface. The tray is receivable within a processing chamber such that the bed plate extends through the opening in the bottom surface of the tray to support the bioreactor vessel. 112-. (canceled)13. A system for bioprocessing , comprising:a tray having a plurality of sidewalls and a bottom surface defining an interior compartment, and a generally open top; andat least one opening formed in the bottom surface, the opening having a perimeter;wherein the perimeter of the opening is shaped and/or dimensioned such that a bioreactor vessel can be positioned above the opening and supported by the bottom surface of the tray within the interior compartment while a portion of the bioreactor vessel is accessible through the opening in the bottom surface.14. The system of claim 13 , wherein:the perimeter of the opening includes at least one tab or projection for supporting the bioreactor vessel above the opening.15. The system of claim 14 , wherein:the at least one opening is at least two openings formed in the bottom surface, each opening having a perimeter shaped and/or dimensioned for supporting a bioreactor above each opening, respectively.16. The system of claim 13 , further comprising:a plurality of bosses extending upwardly from the bottom surface of the tray for inhibiting lateral movement of the bioreactor vessel when it is received above the opening in the bottom surface of the tray.17. The system of claim 13 , further comprising:a first tubing holder block associated with ...

Bioprocessing methods for cell therapy

A bioprocessing method for cell therapy includes the steps of genetically modifying a population of cells in a bioreactor vessel to produce a population of genetically modified cells, and expanding the population of genetically modified cells within the bioreactor vessel to generate a number of genetically modified cells sufficient for one or more doses for use in a cell therapy treatment without removing the population of genetically modified cells from the bioreactor vessel. Cells are settled on a gas permeable, liquid impermeable membrane for expansion, and are resuspended when, or after, the desired cell density is reached.

PRODUCT QUALITY ATTRIBUTE MEASUREMENT

Systems for measuring a product quality attribute of an analyte of a biological sample include a first flow control device, a sample purification device, a second flow control device in fluid communication with first and second sample analyzers, where the first sample analyzer includes a first chromatography column, and a control unit configured so that during operation of the system, the control unit adjusts a configuration of the second flow control device to direct a portion of the biological sample to one of the first and second sample analyzers, and determines a product quality attribute of an analyte of the biological sample based on an analysis of the portion of the biological sample by the one of the first and second sample analyzers. 1. A system for measuring a product quality attribute of an analyte of a biological sample , the system comprising:a first flow control device;a sample purification device in fluid communication with the first flow control device;a second flow control device in fluid communication with the first flow control device, with the sample purification device, and with first and second sample analyzers, wherein the first sample analyzer comprises a first chromatography column; and (a) adjusts a configuration of the first flow control device to direct a portion of a biological sample from the first flow control device into either the sample purification device or into the second flow control device, so that the portion of the biological sample is received in the second flow control device;', '(b) adjusts a configuration of the second flow control device to direct the portion of the biological sample to one of the first and second sample analyzers; and', '(c) determines a product quality attribute of an analyte of the biological sample based on an analysis of the portion of the biological sample by the one of the first and second sample analyzers., 'a control unit coupled to the first and second flow control devices and configured so ...

AUTOMATED CELL CULTURE INCUBATOR

Aspects relate to an automated cell culture incubator and to methods for using such an incubator. In one aspect, the cell culture incubator includes an incubator cabinet having a transfer chamber and an internal chamber that are arranged to form an airlock configuration. In some embodiments, objects placed into the incubator cabinet are sterilized using ozone produced by an ozone generator. The incubator may include one or more transfer devices that move objects between the transfer chamber and the internal chamber. 1. A cell culture incubator comprising:an incubator cabinet comprising (i) a transfer chamber and (ii) an internal chamber;a sterilization medium source;a pump operatively coupled with said sterilization medium source and configured for supplying sterilization medium to said transfer chamber;an external door opening from an external environment to said transfer chamber;an internal door opening from said transfer chamber to said internal chamber; anda transfer device for moving one or more items between said transfer chamber and said internal chamber.2. The incubator of claim 1 , wherein said internal chamber is configured to store a plurality of cell culture vessels.3. The incubator of or claim 1 , wherein the sterilization medium source is in fluid communication with said internal chamber.4. The incubator of any one of - claim 1 , wherein said external door forms a substantially gas-tight seal when closed.5. The incubator of any one of - claim 1 , wherein said internal door forms a substantially gas-tight seal when closed.6. The incubator of any one of - claim 1 , wherein said transfer chamber is configured to accept a cell culture vessel.7. The incubator of any one of - claim 1 , further comprising a controller of said transfer device for moving one or more items.8. The incubator of claim 7 , wherein said controller is located exterior to said incubator cabinet.9. The incubator of or claim 7 , wherein said controller comprises a computer.10. The ...

NON-POWERED CONSTANT-TEMPERATURE CELL TRANSFER DEVICE

The present invention relates to a non-powered constant-temperature cell transfer device, in which a first container, which accommodates living animal cells and a culture solution, is accommodated in a second container having a heat generating unit that emits heat generated by oxidation and reduction reactions of metal by introducing oxygen in the atmosphere in order to continuously provide an optimum culture temperature to a culture container even without being supplied with electric power, thereby maintaining activity and viability of the cells by maintaining a culture environment optimal for proliferation of the cells accommodated in the first container. 1. A non-powered constant-temperature cell transfer device , wherein a first container accommodating living animal cells and a culture solution is accommodated in a second container having a heat generating unit that emits heat generated by oxidation and reduction reactions of metal by introducing oxygen in the atmosphere , such that the first container is transferred while maintaining a physiologically active temperature of the cells accommodated in the first container.2. A non-powered constant-temperature cell transfer device , wherein a third container , which accommodates a first container accommodating living animal cells and a culture solution so as to maintain a horizontal state of the first container , is installed in a second container having a heat generating unit that emits heat generated by oxidation and reduction reactions of metal by introducing oxygen in the atmosphere , such that the cells are transferred while maintaining a physiologically active temperature of the cells accommodated in the first container by the second container , and an inclination of the first container is minimized by the third container.3. A non-powered constant-temperature cell transfer device , wherein a third container , which accommodates a first container accommodating living animal cells and a culture solution so as to ...

INDEPENDENT PORTS IN AUTOMATED BIOREACTOR SYSTEM

A totipotent plant tissue multiplication system can include a sterile enclosure and a bioreactor in the sterile enclosure. The bioreactor can include a number of ports. A sensor can be inserted into a first port. A matter insertion system can input matter into the bioreactor via tubing configured to be connected to a second port. The sensor can be removed from the first port during operation of the matter insertion system. The tubing can be disconnected from the second port during operating of the sensor and the culture transfer system. 1. A system for performing totipotent plant tissue multiplication , the system comprising:a sterile enclosure;a bioreactor in the sterile enclosure, the bioreactor comprising a plurality of ports;at least one sensor inserted into a first port of the plurality of ports;a matter insertion system configured to input matter into the bioreactor via a first tubing configured to be connected to a second port of the plurality of ports; andwherein the at least one sensor can be removed from the first port during operation of the matter insertion system, and wherein first tubing can be disconnected from the second port during operating of the at least one sensor.2. The system of claim 1 , wherein the at least one sensor comprises one or more of a dissolved gas sensor claim 1 , a sugar sensor claim 1 , a pH sensor claim 1 , an optical density sensor claim 1 , or a temperature sensor.3. The system of claim 1 , wherein the at least one sensor is in communication with a controller and wherein the controller is in communication with the matter insertion system.4. The system of claim 1 , wherein the matter insertion system is configured to control input of the matter into the bioreactor based on signals from the at least one sensor.5. The system of claim 1 , wherein the matter comprises one or more of medium claim 1 , acidic material claim 1 , basic material claim 1 , or gasses.6. The system of claim 1 , wherein the matter comprises gasses claim 1 , ...

CULTURE HARVESTING IN AUTOMATED BIOREACTOR SYSTEM

A system for harvesting totipotent plant tissue culture can include a sterile enclosure, a plurality of bioreactors in the sterile enclosure, at least one agitator in the sterile enclosure, and a culture harvest system. The at least one agitator can be configured to agitate culture within the plurality of bioreactors. The culture harvest system can include tubing configured to connect to a port of one of the plurality of bioreactors in the sterile enclosure, a culture harvest container, and a pump configured to pump culture from the one of the plurality of bioreactors into the culture harvest container via the tubing. 1. A system for harvesting totipotent plant tissue culture , the system comprising:a sterile enclosure;a plurality of bioreactors in the sterile enclosure;at least one agitator in the sterile enclosure, the at least one agitator configured to agitate culture within the plurality of bioreactors; and tubing configured to connect to a port of one of the plurality of bioreactors in the sterile enclosure,', 'a culture harvest container, and', 'a pump configured to pump culture from the one of the plurality of bioreactors into the culture harvest container via the tubing., 'a culture harvest system, comprising2. The system of claim 1 , wherein the port comprises a cap configured to cover the port when the port is not connected to the tubing.3. The system of claim 2 , wherein the system further comprises:a clamp configured to be clamped to the port during a time between removal of the cap and connection of the tubing.4. The system of claim 1 , further comprising:a weighing device configured to weigh culture harvested from the one of the plurality of bioreactors into the culture harvest container.5. The system of claim 4 , further comprising:a controller in communication with the weighing device, wherein the controller is configured to store, in a database, information about the culture harvested from the one of the plurality of bioreactors into the culture ...

DYNAMIC POLYMER SURFACES FOR SCREENING, ENRICHMENT, AND HARVESTING OF CELLS AND OTHER SOFT COLLOIDAL PARTICLES

Dynamic polymer surfaces are provided that include alternating micropatterns of adhesive domains and environmental stimuli-responsive repulsive domains, where application of a select environmental stimulus activates polymer structures of the repulsive domains to change conformation with respect to the adhesive domains. The dynamic polymer surfaces are useful for sorting, screening, and enriching target particles (such as cells) in a sample and for culturing and harvesting cells. Products, such as cell culture systems, including the dynamic polymer surfaces are also provided.

Devices and methods for isolating a migratory cell population

A method and device, for isolating a migratory cell population from a mixed population of migratory cells and non-migratory cells is described herein. The method comprises placing the mixed population of migratory cells and non-migratory cells in contact with a device having one or more microchannels; and isolating the migratory cell population within the microchannels following an incubation period, wherein the one or more microchannels have an average size in a proliferation direction of the migratory cells that is less than a minimum proliferation space of the migratory cells along the proliferation direction.

INSTRUMENTS, MODULES, AND METHODS FOR IMPROVED DETECTION OF EDITED SEQUENCES IN LIVE CELLS

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited. 1. A singulation assembly for a solid wall singulation or substantial singulation , growth , induction of editing , and normalization or cherry-picking (SWIIN) module comprising:a retentate member comprising an upper surface and a lower surface, wherein the retentate member comprises at least one retentate distribution channel which traverses the retentate member from its upper surface to its lower surface and for 70% to 95% of a length of retentate member; wherein the lower surface of the retentate member comprises flow directors; wherein the retentate member further comprises one or more retentate member ports configured to supply cells and fluid to and remove cells and fluid from the retentate member; and wherein the retentate member ports are fluidically-connected to the at least one retentate distribution channel and retentate flow directors;a perforated member with an upper surface and a lower surface, wherein the upper surface of the perforated member is positioned beneath and adjacent to the lower surface of the retentate member and wherein the perforated member comprises at least 25,000 perforations;a filter with an upper surface and a lower surface, wherein the upper surface of the filter is positioned beneath and adjacent to the lower surface of the perforated member and wherein the lower surface of the filter is positioned above and adjacent to an upper surface of a permeate member; andthe permeate member comprising the upper surface and a lower surface, wherein the permeate member comprises at least one permeate distribution channel which traverses the permeate member from its ...

METHOD OF MANUFACTURING A CULTURE SUPPORT HAVING IMPROVED CELL ADHESIVENESS AND MOBILITY

Provided is a method of manufacturing a cell culture support having improved cell adhesion and mobility. The method includes: mixing a hydrophilic polymer, a hydrophobic polymer and a solvent to prepare an electro-spinning solution having a viscosity from 50 cps to 2000 cps; electro-spinning the electro-spinning solution to form polymer fibers having beads formed on each of the fibers; accumulating the polymer fibers to form a fibrous web having pores; and penetrating a culture solution into the pores of the fibrous web. The beads have a diameter larger than that of the polymer fibers. 1. A method of manufacturing a cell culture support having improved cell adhesion and mobility , the method comprising:mixing a hydrophilic polymer, a hydrophobic polymer and a solvent to prepare an electro-spinning solution having a viscosity from 50 cps to 2000 cps;electro-spinning the electro-spinning solution to form polymer fibers having beads formed in each of the polymer fibers;accumulating the polymer fibers to form a fibrous web having pores; andpenetrating a culture solution into the pores of the fibrous web.2. The method of claim 1 , wherein the polymer fibers have a diameter in a range from 100 nm to 10 μm.3. The method of claim 2 , wherein the beads have a diameter larger than that of the polymer fibers.4. The method of claim 1 , wherein the polymer fibers contain 60 wt % to 90 wt % of the hydrophilic polymer.5. The method of claim 1 , wherein the hydrophilic polymer is PVP (polyvinylpyrrolidone) or PAN (polyacrylonitrile).6. The method of claim 1 , wherein the hydrophobic polymer is one of PVdF (polyvinylidene fluoride) claim 1 , PU (polyurethane) claim 1 , and PES (polyethersulfone).7. The method of claim 1 , wherein the solvent is at least one selected from the group consisting of DMAc (N claim 1 ,N-dimethyl acetoamide) claim 1 , DMF (N claim 1 ,N-dimethylformamide) claim 1 , NMP (N-methyl-2-pyrrolidinone) claim 1 , DMSO (dimethyl sulfoxide) claim 1 , THF (tetra- ...

Devices, Systems, and Methods for the Fabrication of Tissue

Described herein are bioprinters comprising: one or more printer heads, wherein a printer head comprises a means for receiving and holding at least one cartridge, and wherein said cartridge comprises contents selected from one or more of: bio-ink and support material; a means for calibrating the position of at least one cartridge; and a means for dispensing the contents of at least one cartridge. Further described herein are methods for fabricating a tissue construct, comprising: a computer module receiving input of a visual representation of a desired tissue construct; a computer module generating a series of commands, wherein the commands are based on the visual representation and are readable by a bioprinter; a computer module providing the series of commands to a bioprinter; and the bioprinter depositing bio-ink and support material according to the commands to form a construct with a defined geometry. 110.-. (canceled)12. The bio-ink cartridge of claim 11 , wherein the bio-ink is a homologous ink.13. The bio-ink cartridge of claim 11 , wherein the bio-ink is a heterologous ink.14. The bio-ink cartridge of claim 11 , wherein the plurality of human cells further comprise differentiated cells.15. The bio-ink cartridge of claim 11 , wherein the plurality of human cells further comprise non-differentiated cells.16. The bio-ink cartridge of claim 11 , wherein the plurality of human cells further comprise a mixture of differentiated cells and non-differentiated cells.17. The bio-ink cartridge of claim 15 , wherein the non-differentiated cells comprise stem cells.18. The bio-ink cartridge of claim 17 , wherein the stem cells comprise progenitor cells.19. The bio-ink cartridge of claim 17 , wherein the stem cells comprise pluripotent cells.20. The bio-ink cartridge of claim 11 , wherein the cell culture media comprises lipids.21. The bio-ink cartridge of claim 11 , wherein the cell culture media comprises cytokines.22. The bio-ink cartridge of claim 11 , wherein the ...

INSTRUMENTS, MODULES, AND METHODS FOR IMPROVED DETECTION OF EDITED SEQUENCES IN LIVE CELLS

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited. 1. A compound singulation or substantial singulation , growth , induction of editing , and normalization or cherry-picking (SWIIN) module , wherein the SWIIN module comprises at least two singulation assemblies , wherein each singulation assembly comprises:a retentate member comprising an upper surface and a lower surface, wherein the retentate member comprises at least one retentate distribution channel which traverses the retentate member from its upper surface to its lower surface; wherein the lower surface of the retentate member comprises a first portion of a serpentine channel; wherein the retentate member further comprises one or more retentate member ports configured to supply cells and fluid to and remove cells and fluid from the retentate member; and wherein the retentate member ports are fluidically-connected to the at least one retentate distribution channel and the first portion of the serpentine channel;the permeate member comprising the upper surface and a lower surface, wherein the permeate member comprises at least one permeate distribution channel which traverses the permeate member from its lower surface to its upper surface; wherein the upper surface of the permeate member comprises a second portion of the serpentine channel; wherein the permeate member further comprises one or more permeate member ports configured to supply fluid to and remove fluid from the permeate member; wherein the permeate member ports are fluidically-connected to the second portion of the serpentine channel and permeate flow directors; and wherein the first and second portions of the serpentine ...

HIGH DENSITY SELF-CONTAINED BIOLOGICAL ANALYSIS

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR. 127.-. (canceled)28. A method of nucleic acid extraction and multiplex PCR in a self-contained system , comprising: i. one or more ports, including an injector port for introducing the sample into the self-contained system,', 'ii. a nucleic acid preparation zone, the nucleic acid preparation zone configured for purifying nucleic acids,', 'iii. at least one amplification zone, the amplification zone configured for amplification of the plurality of nucleic acids that may be in the sample;, '(a) providing the self-contained system having, in fluid communication(b) introducing the sample into the self-contained system via the injector port, and closing the injector port subsequent to introducing the sample into the self-contained system;(c) preparing the nucleic acids in the nucleic acid preparation zone, and moving the nucleic acids into the amplification zone;(d) mixing the nucleic acids in the sample with PCR reaction components including a plurality of primer pairs configured for amplifying the targets,(e) thermal cycling the nucleic acids in the amplification zone to create an amplification mixture, and(f) detecting which of the plurality of nucleic acids are present in the amplification mixture.29. The method of claim 28 , wherein the ports are sealable ports that provide the only access from an exterior of the container such that when all of the one or more sealable ports are closed claim 28 , the container is fully closed claim 28 , the method comprising (g) closing the sealable ports claim 28 , wherein step (g) takes place after step (b).30. The method of claim 28 , wherein at least one of the ports is a one-way valve.31. The method of wherein the container further comprises a cell lysis zone configured for lysing cells or spores located in the ...

BIOPROCESSING

Bioreactors are provided that include a vessel and a jet mixer disposed in the vessel. Methods that utilize the bioreactors are provided, involving placing a microorganism or cells and a fluid medium in the bioreactor. 1. A bioreactor comprisinga vessel,a jet mixing system configured to draw fluid contents from the vessel, through an inlet in a wall of the vessel to piping that is external to the vessel, and jet the fluid contents from the piping back into the vessel,a gas delivery system configured to supply a process gas to the vessel, anda temperature control system configured to regulate temperature in the vessel.2. The bioreactor of further comprising a plurality of sensors.3. The bioreactor of further comprising a process controller configured to receive input from the sensors.4. The bioreactor of further comprising delivery devices configured to deliver to the vessel a medium claim 1 , microorganisms or cells claim 1 , and/or other materials used in a bioprocess.5. The bioreactor of wherein the vessel includes a vent claim 1 , and the bioreactor includes a source of oxygen in communication with the vessel claim 1 , an oxygen monitor configured to monitor the oxygen content of a liquid in the vessel claim 1 , and a controller configured to adjust the oxygen content of the liquid claim 1 , using the vent and oxygen source claim 1 , in response to input from the oxygen monitor.6. The bioreactor of further comprising a concentration monitor configured to monitor the concentration of a product in the liquid in the vessel claim 1 , and a controller configured to stop a bioprocess taking place within the vessel based on input received from the concentration monitor.7. The bioreactor of wherein the vessel includes an outlet through which effluent can be collected.8. The bioreactor of wherein the jet mixing system is configured to limit any increase in the overall temperature of a fluid medium in the vessel to less than 5 degrees C. over the course of mixing.9. The ...

3D Printing Of A Cellularised Scaffold

The present invention relates to a process for producing a droplet assembly, which droplet assembly comprises a plurality of droplets, wherein each of said droplets comprises: an aqueous medium comprising a hydrogel compound; and one or more biological cells disposed in the aqueous medium, which process comprises: generating, in a bulk hydrophobic medium, a plurality of droplets, wherein each of said droplets comprises: an aqueous medium comprising a hydrogel compound; and one or more biological cells disposed in the aqueous medium. The invention also relates to a droplet assembly comprising a plurality of droplets, wherein each of said droplets comprises: (i) an aqueous medium comprising a hydrogel compound; (ii) one or more biological cells disposed in the aqueous medium; and (iii) an outer layer of amphipathic molecules around the surface of the aqueous medium, wherein at least one droplet in the droplet assembly contacts at least one other droplet in the droplet assembly forming a layer of amphipathic molecules as an interface between contacting droplets.

INSTRUMENTS, MODULES, AND METHODS FOR IMPROVED DETECTION OF EDITED SEQUENCES IN LIVE CELLS

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited. 1. A method for enriching edited cells during CRISPR editing comprising:transforming cells with one or more vectors comprising a promoter driving transcription of a coding sequence for a CRISPR nuclease, an inducible promoter driving transcription of a guide nucleic acid sequence covalently-linked to a DNA donor sequence and wherein each of the one or more vectors comprises a gene for a selectable marker;diluting the transformed cells to a cell concentration to substantially singulate the transformed cells on a substrate;growing the cells on the substrate for at least 5 doublings;initiating editing by inducing the inducible promoter driving transcription of the guide nucleic acid; andgrowing the cells to form colonies of terminal size.2. The method of claim 1 , wherein the inducible promoter is a pL promoter.3. The method of claim 1 , wherein all of the coding sequence for the CRISPR nuclease claim 1 , guide nucleic acid sequence covalently-linked DNA donor sequence are on the same vector.4. The method of claim 1 , wherein the coding sequence for the CRISPR nuclease is on a first vector and the guide nucleic acid sequence and DNA donor sequence are on a second vector.5. The method of claim 1 , wherein the DNA donor sequence further comprises a PAM-altering sequence.6. The method of claim 1 , wherein the cells are mammalian cells.7. The method of claim 1 , further comprising adding selective agents to the substrate to select for the selectable marker(s) on the one or more vectors.8. The method of claim 1 , wherein the cells are grown for at least 20 doublings.9. The method of claim 1 , wherein ...

APPARATUS FOR FLUID LINE MANAGEMENT IN A BIOPROCESSING SYSTEM

In an embodiment, a tubing module for a bioprocessing system includes a first tubing holder block configured to receive at least one pump tube and hold the at least one pump tube in position for selective engagement with a peristaltic pump, a second tubing holder block configured to receive a plurality of pinch valve tubes and hold each pinch valve tube of the plurality of pinch valve tubes in position for selective engagement with a respective actuator of a pinch valve array, and wherein the first tubing holder block and the second tubing holder block are interconnected. 115-. (canceled)16. A system for bioprocessing , comprising:a tray having a plurality of sidewalls and a bottom surface defining an interior compartment, and a generally open top, the tray being configured to receive a bioreactor vessel;a pump assembly positioned adjacent to the rear sidewall of the tray;a pinch valve array positioned adjacent to the rear sidewall of the tray; and a first tubing holder block configured to receive at least one pump tube and hold the at least one pump tube in position for selective engagement with the pump assembly; and', 'a second tubing holder block configured to receive a plurality of pinch valve tubes and hold each pinch valve tube of the plurality of pinch valve tubes in position for selective engagement with a respective actuator of the pinch valve array., 'a tubing module positioned at a rear of the tray, the tubing module including17. The system of claim 16 , wherein:the tubing module is integral with the tray.18. The system of claim 16 , wherein:the tubing module is removably received by an opening formed in a rear wall of the tray.19. The system of claim 16 , further comprising:a temperature controlled processing chamber having a plurality of sidewalls, a bottom surface, and a generally open top, wherein the tray is receivable by the processing chamber;wherein the processing chamber includes a shoe that extends upwardly from the processing chamber and is ...

Producing Liquid Fertilizer in a Biogas Plant

A biogas plant produces both methane and liquid fertilizer by fermenting biomass. The plant includes a fermenter, a percolate tank and a sanitation tank located inside the percolate tank. Dry fermentation takes place in the fermenter and generates methane and a percolate. The percolate is circulated between the fermenter and the percolate tank. Percolate that is returned from the percolate tank to the fermenter is sprinkled over the biomass. A portion of the percolate is transferred from the percolate tank into the sanitation tank. The percolate in the sanitation tank is sanitized by heating to a Celsius temperature between 45° and 65° for a period of at least five days. The percolate in the sanitation tank is heated using both a heating device in the sanitation tank as well as heat generated from a thermophilic fermentation reaction occurring in the percolate tank. The sanitized percolate is used as liquid fertilizer. 112-. (canceled)13. A method for producing liquid fertilizer , comprising:fermenting biomass in a fermenter using dry fermentation, wherein the fermenting generates percolate;circulating the percolate between the fermenter and a percolate tank;transferring a first portion of the percolate from the percolate tank into a sanitation tank;sanitizing the percolate contained in the sanitation tank using a thermophilic fermentation reaction at a Celsius temperature between 45° and 65° for a period of at least five days; anddraining the sanitized percolate as the liquid fertilizer from the sanitation tank.14. The method of claim 13 , further comprising:transferring a second portion of the percolate from the percolate tank into the sanitation tank after the draining of the sanitized percolate from the sanitation tank.15. The method of claim 13 , wherein the first portion of the percolate is transferred from the percolate tank into the sanitation tank when a fill level sensor indicates that the sanitation tank is empty.16. The method of claim 13 , wherein the ...

System and Method for Printing Tissue

A system and method for printing cells in a medium. A multi-dimensional printer, stably constructed of low-mass parts, can include a computer numerically controlled system that can enable motors driving delivery systems. The motors can include encoders that can enable achieving arbitrary resolution. The motors can drive ballscrews to enable linear motion of delivery systems, and the delivery systems can enable printing of a biological material in a pre-selected pattern in a petri dish. The petri dish can accommodate a medium such as a gel, and can further accommodate a vision system that can detect actual position and deflection of the delivery system needle. The printer can accommodate multiple delivery systems and therefore multiple needles of various sizes. 1. A bioprinting system for multi-dimensional printing of tissue into a tissue enclosure , the bioprinting system comprising:a multi-axis, multi-dimensional (MAMD) printer including a robot controller controlling motion of the MAMD printer, and a delivery device operably coupled with the MAMD printer, the delivery device delivering the tissue;the tissue enclosure including a space for the delivered tissue, the tissue enclosure receiving the delivered tissue, the tissue enclosure including a plurality of control points enabling the delivery device to enter the tissue enclosure, the tissue enclosure including production line mounting features; accessing a design of the tissue;', 'converting the design to robot coordinates that the MAMD printer can use to print the tissue into the tissue enclosure;', 'transferring the robot coordinates from the computer to the MAMD printer; and', 'printing the tissue into the tissue enclosure by commanding the MAMD printer based on the robot coordinates., 'a computer executing instructions including2. The bioprinting system as in wherein the instructions comprise:accessing parameters associated with the design;pathing of the design based on the parameters, the pathing producing ...

BIOREACTOR ARRAY AND METHODS OF COMBINATORIAL TESTING

Methods and apparatus for culturing microorganisms are described, including culturing in mixotrophic culture conditions. A bioreactor array with multiple culture vessels with independently controllable inputs is used to culture similar cultures of microorganisms in which at least one parameter differs from other culture vessels in the bioreactor array. 120-. (canceled)21. A method for evaluating the feasibility of large scale microalgae culture , comprising:a. providing a plurality of microalgae cultures in an array of closed culturing vessels, wherein (1) each vessel of the array comprises (a) a plurality of sensors selected from the group consisting of a pH sensor, a temperature sensor, a light sensor, a dissolved oxygen sensor, a dissolved carbon dioxide sensor, and an optical density sensor and (b) a plurality of controls selected from controls for pH, light, gas levels, media levels, temperature, culture agitation, and culture harvesting; (b) the culturing vessels are linked to one or more shared reservoirs for controllably adding culture media, gas, or both to the vessels; and (c) each culture comprises at least 0.5 grams of microalgae in a volume of at least 500 ml of an aqueous culture medium;b. independently subjecting each microalgae culture to one or more related test conditions wherein at least a plurality of the cultures is subjected to different degrees of at least one test condition; andc. evaluating the impact of the test conditions on the cultures to determine the effect of the test conditions on the microalgae and assessing the feasibility of performing large scale commercial microalgae culture under one or more production conditions related to the test conditions from the results of the evaluation.22. The method of claim 21 , wherein the method comprises contemporaneously culturing a plurality of test cultures and a plurality of control cultures in the array.23. The method of claim 22 , wherein the method comprises contemporaneously culturing a ...

Symbiotic Algae System

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, biofuel manufacture, and desirably impacts alternative/renewable energy production, nutrient recovery from waste streams, and valued byproducts production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal-oil extraction waste or additional algae harvested as feedstock for fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as biofuels, fertilizer, etc. to compete with petroleum products in the marketplace. 1. A symbiotic algae system comprising:a first algal growth component, wherein the first algal growth component includes a heterotrophic organism, and wherein the first algal growth component produces a first effluent and an off-gas; anda second algal growth component is fluidly coupled to the first algal growth component, and the second algal growth component including at least one organism from the group of: a photoautotrophic organism, a mixotrophic organism, and a heterotrophic organism, andwherein the second algal growth component receives, as an input, the first effluent and the off-gas and produces a second effluent.2. A symbiotic algae system according to claim 1 , wherein the first algal growth component claim 1 , receives claim 1 , as a first input claim 1 , an effluent input or a waste input.3. A symbiotic algae system according to claim 2 , wherein the second algal growth component receives claim 2 , as a second input claim 2 , an effluent input or a waste input.4. A symbiotic algae system according to claim 1 , further including a waste nutrient preparation sub-system fluidly coupled to the first algal growth component.5. ...

Bioprocessing

Bioreactors are provided that include a vessel and a jet mixer disposed in the vessel. Methods that utilize the bioreactors are provided, involving placing a microorganism or cells and a fluid medium in the bioreactor.

SYSTEM AND METHOD FOR BUOYANT PARTICLE PROCESSING

A system for buoyant particle processing includes: a reaction vessel, a stirring mechanism, a set of one or more pumps, and a filter. The system can additionally or alternatively include a set of pathways and/or any other suitable component(s). A method for buoyant particle processing includes: stirring the contents of a reaction vessel; washing a set of buoyant particles; and filtering the contents of the reaction vessel. Additionally or alternatively, the method can include any or all of: preprocessing the set of buoyant particles; adding a set of inputs to the reaction vessel; washing the set of buoyant particles; repeating one or more; and/or any other suitable process(es). 1. A method for processing a set of buoyant particles , the method comprising: a wash buffer;', 'a set of processing materials; and', 'the set of buoyant particles, wherein the set of buoyant particles comprises a set of hollow microspheres having a density less than a density of the wash buffer;, 'at a reaction vessel, receivingwith a stirring subsystem comprising a stir rod and an impeller, wherein the impeller is arranged within the reaction vessel, stirring the wash buffer, the set of processing materials, and the set of buoyant particles, wherein the stirring subsystem is configured to facilitate bonding of the set of processing materials with the set of buoyant particles, thereby forming a set of bound buoyant particles; receiving, at an inlet of the tangential flow filter, the set of bound buoyant particles from the reaction vessel, wherein the inlet is arranged at a first height;', 'with a pump, passing the set of bound buoyant particles through an outlet of the tangential flow filter, the outlet arranged at a second height, wherein the second height is arranged superior to the first height, and wherein each of the set of hollow fibers is tilted relative to vertical with a nonzero tilt angle such that the set of bound buoyant particles is directed toward the outlet during a passage ...

ROTARY DEVICE FOR BIO-PRINTING AND METHOD FOR USING THE SAME

The present invention relates to a rotary rod for 3D bio-printing, in which the rotary rod is arranged horizontally and is driven to rotate, the rotary rod has a hollow structure and provided with at least one hole in a surface thereof, such that during a 3D bio-printing process, a nutrition solution passes through the hollow structure and a portion of the nutrition solution exudes via at least one hole. The present invention further provides a 3D bio-printing platform for supplying nutrition, comprising the rotary rod and a nutrition supply system, and a method of printing a tubular tissue using the bio-printing platform. The present invention, which reduces the possibility of resulting in tissue collapse from the effect of gravity, provides a new method of 3D bio-printing a tubular tissue and supplying nutrition in a printing process, with a wide application prospect. 1. A rotary rod for 3D bio-printing , wherein the rotary rod is arranged horizontally and is driven to rotate , the rotary rod has a hollow structure and provided with at least one hole in a surface thereof , such that during a 3D bio-printing process , a nutrition solution passing through the hollow structure and a portion of the nutrition solution exuding via said at least one hole.2. The rotary rod of claim 1 , wherein the rotary rod is rotatably driven by a motor having a controllable rotation speed claim 1 , and at least one end of the rotary rod being detachable.3. The rotary rod of claim 1 , wherein the surface of the rotary rod is coated with at least one layer of liquid-permeable biocompatible substance.4. The rotary rod of claim 3 , wherein said biocompatible substance comprises a biocompatible hydrogel or a porous polymeric film.5. The rotary rod of claim 4 , wherein the biocompatible hydrogel is removable or separable by a biological claim 4 , physical or chemical method comprising temperature control claim 4 , pH adjustment claim 4 , enzymolysis and chemical reaction.6. The rotary rod of ...

CLOSED CULTURE VESSEL FOR ADHERENT CELLS

The present invention provides a culture vessel with which cells can be aseptically cultured and aseptically transferred to a place where the cells are to be used (such as an operating room) and from which the cells can be simply taken out. The present invention provides a closed culture vessel including an openable airtight vessel, and a cell culture member disposed in the airtight vessel and including a cell culture surface, in which the cell culture member is provided detachably from the airtight vessel. 1. A closed culture vessel for adherent cells , comprising:an openable airtight vessel; anda cell culture member disposed in the airtight vessel and having a cell culture surface,wherein the cell culture member is provided detachably from the airtight vessel.2. The culture vessel according to claim 1 , wherein the cell culture member includes: a bottom surface having the cell culture surface; and a side surface extending upward from a circumferential edge of the bottom surface.3. The culture vessel according to claim 2 , wherein the side surface extending upward from the circumferential edge of the bottom surface of the cell culture member is non-cell-adhesive.4. The culture vessel according to claim 1 , wherein the cell culture member is a substrate having the cell culture surface.5. The culture vessel according to claim 1 , wherein the airtight vessel is a vessel including gas exchange means capable of aseptic gas exchange for aseptically performing gas exchange between inside and outside of the airtight vessel.6. The culture vessel according to claim 5 , wherein the gas exchange means capable of aseptic gas exchange is a filter capable of performing gas exchange between inside and outside of the airtight vessel.7. The culture vessel according to claim 5 , wherein the airtight vessel is a gas permeable cell culture bag.8. The culture vessel according to claim 1 , wherein only the cell culture surface of the cell culture member is cell-adhesive and/or another ...

PROCESSING BIOMASS

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material. 1. A method of making a product comprising:determining the lignin content of a biomass feedstock;treating the biomass feedstock material with a process selected from the group consisting of mechanical treatment, chemical treatment, radiation, sonication, pyrolysis, oxidation, steam explosion and combinations thereof; andsetting a process parameter of the process based on the lignin content.2. The method of claim 1 , wherein the treating step comprises irradiating with ionizing radiation.3. The method of claim 2 , wherein the setting step comprises setting the dosage of ionizing radiation to be delivered to the feedstock material.4. The method of claim 3 , wherein 0.1 Mrad to 5.0 Mrad is delivered per 1% by weight of lignin in the biomass feedstock.5. The method of wherein 0.25 Mrad to 4.0 Mrad is delivered per 1% by weight of lignin in the biomass feedstock.6. The method of wherein 0.3 Mrad to 3.5 Mrad is delivered per 1% by weight of lignin in the biomass feedstock.7. The method of wherein treating with ionizing radiation comprises treating with an electron beam.8. The method of further comprising converting at least a portion of the treated biomass feedstock claim 1 , utilizing a microorganism claim 1 , to produce a product.9. The method of claim 8 , wherein the product comprises an alcohol.10. The method of claim 8 , wherein the product comprises a carboxylic acid claim 8 , ester of carboxylic acid claim 8 , salt of carboxylic acid claim 8 , or a mixture thereof.11. The method of claim 1 , further comprising repeating the determining claim 1 , treating and setting steps with a second feedstock.12. ...

Biomanufacturing System, Method, and 3D Bioprinting Hardware in a Reduced Gravity Environment

A method, apparatus, and system are provided for the printing and maturation of living tissue in an Earth-referenced reduced gravity environment such as that found on a spacecraft or on other celestial bodies. The printing may be three-dimensional structures. The printed structures may be manufactured from low viscosity biomaterials. 1. An additive manufacturing apparatus comprising:a reduced gravity environment;one or more cell culturing devices in said reduced gravity environment, wherein said one or more cell culturing devices includes an interior;a bioprinter positioned in said reduced gravity environment, wherein said bioprinter has one or more print heads;one or more bioinks in fluid communication with said one or more print heads;said one or more bioinks in fluid communication with the interior of said one or more cell culturing devices to print one or more tissues and wherein said one or more cell culturing devices incubates said one or more tissues.2. The apparatus of wherein said cell culturing device is removable from said bioprinter.3. The apparatus of wherein said cell culturing device includes an integrated life support system for transportation of said cell culturing device from said reduced gravity environment to an environment having a different gravity.4. The apparatus of wherein said one or more tissues is one or more three-dimensional tissues.5. The apparatus of wherein said cell culturing device includes at least one of a mechanical tissue stimulation or electrical tissue stimulation.6. The apparatus of wherein said reduced gravity environment is both temperature and humidity controlled.7. The apparatus of wherein said bioprinter includes thermal control.8. The apparatus of wherein said one or more print heads include thermal control.9. The apparatus of wherein said one or more print heads include one or more dispenser tips claim 8 , wherein the one or more dispenser tips include thermal control.10. The apparatus of wherein said one or more ...

Methods and compositions for formulating and dispensing pharmaceutical formulations

The present disclosure relates to use of specialized vessels for formulating and dispensing pharmaceutical formulations. The vessels are optionally able to maintain homeostatic conditions and a homogeneous constitution of pharmaceutical suspensions, while simultaneously dispensing them into single-use containers.

ELECTROPORATION APPARATUS AND METHOD

Electroporation is a technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane. This allows for chemicals, drugs, and/or macromolecules such as proteins and nucleic acids to be introduced into the cells. Embodiments relate to an electroporation apparatus. The electroporation apparatus comprises: a plurality of chambers configured to store a plurality of cells during an electroporation process; a plurality of electrodes configured to generate a plurality of electric fields within the plurality of chambers during the electroporation process, each electric field of the plurality of electric fields corresponding to one chamber of the plurality of chambers; a flow channel configured to transport the plurality cells during a cell collection process after the electroporation process; and a plurality of valves connecting the plurality of chambers to the flow channel. 1. An electroporation apparatus , comprising:a plurality of chambers configured to store a plurality of cells during an electroporation process;a plurality of electrodes configured to generate a plurality of electric fields within the plurality of chambers during the electroporation process, each electric field of the plurality of electric fields corresponding to one chamber of the plurality of chambers;a flow channel configured to transport the plurality cells during a cell collection process after the electroporation process; anda plurality of valves connecting the plurality of chambers to the flow channel.2. The electroporation apparatus of claim 1 , further comprising:an inlet port;an outlet port; anda plurality of flanking flow channels connecting the inlet port and the outlet port to the flow channel.3. The electroporation apparatus of claim 2 , further comprising:a pump for pumping a liquid medium from the flow channel into at least one of the plurality of chambers during a collection process,wherein the liquid medium is obtained at the inlet ...

Cell stimulating apparatus, cell stimulating method, culture product manufacturing method, isolated cell manufacturing method, and cell growing method

A cell stimulating apparatus for detaching adhesive cells adhered on a culture substrate from the culture substrate by stimulating the cells, comprising: an application unit configured to apply an alternating field between a first electrode and a second electrode opposing each other by sandwiching cells and a solution in which the cells are dipped, wherein at least one of the first electrode and the second electrode is not in contact with the cells and the solution, wherein the cells and the solution are contained in a vessel, and the first electrode and the second electrode are arranged outside the vessel.

SYMBIOTIC ALGAE SYSTEM

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, biofuel manufacture, and desirably impacts alternative/renewable energy production, nutrient recovery from waste streams, and valued byproducts production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal-oil extraction waste or additional algae harvested as feedstock for fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as biofuels, fertilizer, etc. to compete with petroleum products in the marketplace. 1. A symbiotic algae system comprising:a first algal growth component, wherein the first algal growth component includes a heterotrophic organism, and wherein the first algal growth component produces a first effluent and an off-gas; anda second algal growth component fluidly coupled to the first algal growth component, wherein the second algal growth component includes at least one organism from the group of: a photoautotrophic organism, a mixotrophic organism, and a heterotrophic organism, andwherein the second algal growth component receives, as a first input, the first effluent and the first off-gas and produces a second effluent and a second off-gas; andwherein the second effluent and the second off-gas are received as inputs to the first algal growth component.2. A symbiotic algae system according to claim 1 , wherein the first algal growth component claim 1 , receives claim 1 , as an additional input claim 1 , an effluent input or a waste input claim 1 , and wherein the additional input and the second effluent include a nitrogen and a phosphorous.3. A symbiotic algae system according to claim 2 , wherein the first algal ...

PHYSICAL MANIPULATION OF TISSUE CULTURED TISSUE

The disclosure relates to methods, systems and compositions for physically manipulating a muscle tissue culture either mechanically, or manually, or both. Specifically, the disclosure relates to systems and methods of physically manipulating, either mechanically or manually, a resilient container of bioprinted tissue culture having non-random three dimensional cell structure by elongation, compression, torque and shear of the tissue culture. 1. A method of physically manipulating a tissue culture , implementable in a computerized system comprising: at least one resilient container operably coupled to a frame sized and configured to affect a predetermined regimen of stress and strain over at least one of four degrees of freedom on the at least one resilient container , and a central processing module (CPM) in communication with at least one processor and at least one non-volatile memory storage device having thereon a processor-readable media with a set of executable instructions , configured , when executed , to cause the at least one processor to: using a sensor array , receive physical data characteristic of the tissue culture; and based on the data , physically manipulate the at least one resilient container , the method comprising:a. loading the tissue culture into the at least one resilient container; andb. at predetermined intervals, based on the physical data characteristic of the tissue culture, affect at least one of stress, and strain on the at least one resilient container.2. The method of claim 1 , wherein the system further comprises an ink jet bioprinting module havinga. at least one microprocessor in communication with: a non-volatile storage device having thereon a microprocessor-readable medium with set of executable instructions configured to, when executed, to cause the at least one microprocessor to execute a method of inkjet bioprinting; and an image library corresponding to a two dimensional (2D) layer of at least one of cells, scaffolding, and ...