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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 1744. Отображено 100.
10-05-2012 дата публикации

Method and device for thermal hydrolysis and steam explosion of biomass

Номер: US20120111515A1
Автор: Odd Egil Solheim, Pål Jahre NILSEN, Paul Walley
Принадлежит: Individual

It is described a process for thermal hydrolysis and team explosion of biomass, said method comprising the steps of: (i) leading the biomass approximately continuous to a first preheating step and preheat the biomass, (ii) leading the preheated biomass into at least two reactors sequentially, (iii) heating and pressurising the reactor by addition of steam, (iv) keeping the re-actor(s) at a certain temperature and pressure for a certain time, (v) leading the heated and pressurised biomass from the reactor(s) to a first pressure relief tank without any substantial pressure reduction and quickly relief the pressure of the biomass by means of a nozzle, in order to disintegrate the biomass, (vi) leading the biomass from the first pressure relief tank to a second pressure relief tank having a lower pressure than the pressure of the first pressure relief tank, (vii) leading the so treated biomass to a downstream facility for subsequent treatment. The invention also comprises a device for thermal treatment of biomass.

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Номер записи: 1
13-12-2012 дата публикации

Saccharide-solution producing apparatus, fermentation system, saccharide-solution producing method, and fermentation method

Номер: US20120315677A1
Автор: Gaku Kondo, Hideo Suzuki, Michio Nishiyama, Minoru Genta, Seiichi Terakura
Принадлежит: Mitsubishi Heavy Industries Mechatronics Systems Ltd

A saccharide-solution producing apparatus 11 A according to the present invention is a saccharide-solution producing apparatus for producing a saccharide solution 22 derived from a carbohydrate-based material 21 , and includes a saccharide-solution controlling unit 15 A that controls the saccharide solution derived from the carbohydrate-based material 21 , a cellulosic biomass saccharifying unit 16 that saccharifies hydrothermally treated biomass obtained by hydrothermally decomposing a cellulosic biomass material 35 that contains a lignin component and a hemicellulose component, and produces a diluted saccharide solution 37 , and a diluted-saccharide-solution supply pipe L 11 that mixes the diluted saccharide solution 37 produced by the cellulosic biomass saccharifying unit 16 into the saccharide-solution controlling unit 15 A. With this configuration, it is possible to improve production efficiency of the saccharide solution 22 and to realize cost reduction.

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Номер записи: 2
07-03-2013 дата публикации

Recovery of higher alcohols from dilute aqueous solutions

Номер: US20130059370A1
Автор: Aharon M. Eyal, Andrew C. Hawkins, Aristos A. Aristidou, David A. Glassner, Fudu Miao, Kent Evans, Patrick R. Gruber, William A. Evanko
Принадлежит: Gevo Inc

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

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Номер записи: 3
18-07-2013 дата публикации

Treatment of organic waste

Номер: US20130183745A1
Автор: Jorma Oinas, Seppo Aho
Принадлежит: SAVATERRA OY

There is provided an apparatus for assisting hygienization of organic waste. The apparatus comprises at least one reception means for receiving organic solid matter and organic waste, mixing means for mixing the organic waste and the organic solid matter together into an organic mixture, selecting means for selecting whether to expose the organic mixture in the mixing means to either: aeration in order to create a homogenized and internally oxygenous organic mixture, thereby accelerating the hygienization of the organic mixture, or a heat treatment, wherein the heat treatment performs the hygienization of the organic mixture, or to neither the aeration nor the heat treatment. The mixing means may then expose the organic mixture to the aeration, or to the heat treatment, or to neither of the aeration nor the heat treatment on the basis of the selection. The apparatus may further comprise delivery means for passing the organic mixture to either aerobic biodegradation or an end user.

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Номер записи: 4
01-08-2013 дата публикации

Processing biomass

Номер: US20130196386A1
Автор: Marshall Medoff
Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 5
12-09-2013 дата публикации

Integrated Biorefinery

Номер: US20130236941A1
Автор: Burns-Guydish Stacy M., Cann Anthony F., Dalal Dhruti, Fry Lawrence W., Hershkowitz Michael S., McDonald William F., Meyer Andrew D., Nava-Salgado Victor O., Olson Brandon T., Sonico Ishmael M., Walther David C., Zhu Yongming
Принадлежит: COBALT TECHNOLOGIES INC.

Systems and methods for producing bioproducts are shown. The systems and methods herein can be configured and used in an integrated biorefinery. The integrated biorefinery may comprise a sugar production facility such as a sugar mill, a production facility for one or more bioproduct(s) such as butanol, and optionally an ethanol production facility employing the system and method. 1. An integrated biorefinery , comprising:(a) a sugar production facility, wherein sugar-containing biomass is processed to extract sugar, thereby producing a liquid sugar-containing extract and residual bagasse; and(b) a bioproduct production facility, comprising a microorganism that is capable of producing at least one bioproduct in a microbial fermentation process,wherein sugar molecules that are extracted from sugar-containing biomass that is processed in the sugar production facility are provided to the microorganism in a growth medium,wherein the sugar molecules are fermented by the microorganism, thereby producing a fermentation broth that comprises said at least one bioproduct.2. An integrated biorefinery according to claim 1 , wherein the sugar molecules that are provided to the microorganism comprise: (i) at least a portion of the liquid sugar-containing extract claim 1 , and (ii) sugar molecules extracted from at least a portion of the residual bagasse and/or sugar molecules extracted from at least a portion of biomass that is removed from the sugar-containing biomass prior to processing in the sugar production facility.3. An integrated biorefinery according to claim 1 , wherein said sugar-containing biomass comprises sugar cane.4. An integrated biorefinery according to claim 1 , wherein said sugar-production facility comprises a sugar mill.5. An integrated biorefinery according to claim 2 , wherein said sugar-containing biomass comprises sugar cane claim 2 , and wherein said biomass that is removed prior to processing in the sugar production facility comprises cane straw.6. An ...

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Номер записи: 6
26-12-2013 дата публикации

Processing biomass

Номер: US20130344586A1
Автор: Harrison Medoff, Marshall Medoff, Thomas Craig Masterman
Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation.

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Номер записи: 7
30-01-2014 дата публикации

Conditioning chamber for storing samples in a time-controlled manner and method for storing samples in a time-controlled manner

Номер: US20140030802A1
Автор: Gavin Clarke, Klaus-Guenter EBERLE
Принадлежит: Andreas Hettich GmbH and Co KG

The invention relates to an air-conditioning space ( 10 ) for storing samples in a time-controlled manner, comprising a device ( 22 ) for automatically feeding sample containers ( 18 ) into a climatically sealed space ( 12 ) having at least one wall ( 43 ), into which a sample container ( 18 ) can be inserted into the climatically sealed space ( 12 ) through an opening ( 40, 42 ), wherein the feed device ( 22 ) has at least one drive and control unit, and an incubator receptacle ( 30 ) for receiving the samples ( 18 ) is provided inside the climatically sealed space ( 12 ). The invention is distinguished by the fact that the feed device has an automatic feed arm ( 22, 24 ) which grips a sample container ( 18 ) from a receiving position outside the climatically sealed space ( 12 ) and deposits the sample container ( 18 ) there in a clear deposit position in an incubator receptacle ( 30 ).

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Номер записи: 8
14-01-2016 дата публикации

BIOMASS TREATMENT SYSTEM, SACCHARIDE SOLUTION PRODUCING PROCESS USING BIOMASS AS RAW MATERIAL, AND ORGANIC RAW MATERIAL PRODUCING PROCESS

Номер: US20160010051A1
Автор: Genta Minoru, Kobayashi Seiji, Terakura Seiichi, Uehara Ryosuke
Принадлежит:

This biomass treatment system includes: a hydrothermal decomposition section for decomposing the cellulose, hemicellulose and lignin contained in a biomass as a raw material under high-temperature and high-pressure conditions in a main section, namely a tank having a gas-liquid interface, and thus removing a lignin component and a hemicellulose component from the biomass; a discharge section for discharging a biomass solid (a component insoluble in hot-water) obtained by the decomposition; a slurrying tank which is connected to the discharge section and in which the discharged biomass solid is slurried in water fed thereinto to form a biomass slurry; and a solid-liquid separation apparatus including both a settling tank which is provided on a discharge line for discharging the biomass slurry and in which the biomass slurry is settled and a scooping-up and conveying means for scooping up the biomass solid deposited at the bottom of the settling tank and separating the solid biomass component from water. 1. A biomass treatment system comprising:a biomass treatment section comprising a treatment column having a gas-liquid interface by which a biomass raw material containing cellulose, hemicellulose, and lignin is decomposed under high-temperature, and high-pressure conditions, and thus a lignin component and a hemicellulose component are removed from the biomass;a biomass solid discharge section for discharging a biomass solid treated by the biomass treatment section;a slurrying tank connected to the biomass solid discharge section, into which water is poured and the discharged biomass solid is slurried; anda solid-liquid separation apparatus including a settling tank for settling the biomass slurry, the settling tank being provided downstream of the slurrying tank, and a scooping-up and conveying means for separating water while scooping up the biomass solid settled on a bottom of the settling tank.2. The biomass treatment system according to claim 1 , further ...

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Номер записи: 9
03-02-2022 дата публикации

Intelligent Microbial Sample Treatment System

Номер: US20220033760A1
Автор: Linsheng HE, Qiang Yuan
Принадлежит: Chongqing Coretech Medical Technology Co Ltd

An intelligent microbial sample treatment system includes a workbench, and a sample treatment assembly, a culture medium treatment assembly, a streaking assembly and a culture medium storage assembly that are disposed on the workbench; the sample treatment assembly comprises a sample transfer device, a filling device, and a scanning device, a weight detection device, a filling location, a shaking device and waiting locations that are disposed in sequence; and the sample transfer device moves a sample cup among the scanning device, the weight detection device, the filling location, the shaking device and the waiting locations. The intelligent microbial sample treatment system disclosed by the present invention enables improvement of work efficiency and reduction of space occupation and has a reasonable structural layout and high work efficiency.

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Номер записи: 10
21-01-2016 дата публикации

CELL CULTURING DEVICE, CULTURING VESSEL, AND HOLDING VESSEL

Номер: US20160017271A1
Автор: KIYAMA Masaharu, Nakajima Ryota, Nakamura Taku, Nozaki Takayuki, SUGAYA Masakazu, TAKEDA Shizu, TERADA Koichi, ZHOU Guangbin
Принадлежит:

In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vessel holding a biological sample therein, a heat storage portion holding the culture vessel, and a heat insulation portion surrounding the heat storage portion. The whole circumference or a part of , and of the heat insulation portion is removable depending on an arrangement environment.

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Номер записи: 11
18-01-2018 дата публикации

DEVICE FOR THE ELECTRICAL DISINTEGRATION OF CELL STRUCTURES, AND INSTALLATION AND USE OF THE DEVICE FOR PRODUCING FEED INTERMEDIATES AND FEED PRODUCTS

Номер: US20180016541A1
Автор: Fastenau Andreas, Hollah Clemens, Krampe Paul
Принадлежит:

Disclosed is a device for the electrical disintegration of cell structures including a chamber having an inlet for receiving material containing cell structures, an outlet for the discharge thereof, and a conveyor line extending between the inlet and the outlet, an electrode unit which has an electrode body which is arranged within the chamber at least portion-wise along the conveyor line, wherein the chamber has a wall which is portion-wise or completely electrically conductive and electrically insulated from the electrode body, and the electrode unit is adapted to generate an electric field between the electrode body and the wall for electrical disintegration. 1. A device for the electrical disintegration of cell structures , comprisinga chamber having an inlet for receiving material containing cell structures, an outlet for the discharge thereof, and a conveyor line extending between the inlet and the outlet,an electrode unit which has an electrode body which is arranged within the chamber at least portion-wise along the conveyor line, wherein:the chamber has a wall which is portion-wise or completely electrically conductive and electrically insulated from the electrode body,the electrode unit is adapted to generate an electric field between the electrode body and the wall for electrical disintegration, anda conveyor screw is arranged driveably in the conveyor line for conveying the material, wherein the electrode body is accommodated in a cavity in the conveyor screw.2. A device as set forth in claim 1 , wherein the conveyor screw has a hollow shaft and the electrode body is arranged coaxially with the conveyor screw in the hollow shaft.3. A device as set forth in claim 1 , wherein the conveyor screw partly or completely comprises electrically non-conductive material.4. A device as set forth in claim 3 , wherein an electrically non-conductive claim 3 , preferably organic claim 3 , polymer is used as the electrically non-conductive material.5. A device as set ...

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Номер записи: 12
16-01-2020 дата публикации

Non-Pressurised Pre-Treatment, Enzymatic Hydrolysis And Fermentation Of Waste Fractions

Номер: US20200017887A1
Автор: Iversen Frank Krogh, Larsen Jan, Nørholm Nanna Drayer
Принадлежит: RENESCIENCE A/S

The present invention relates to a process for production of fermentation products, including bioethanol by non-pressurised pre-treatment, enzymatic hydrolysis and fermentation of waste fractions containing mono- and/or polysaccharides, having a relatively high dry matter content. The process in its entirety, i.e. from non-pressurised pre-treatment over enzymatic hydrolysis and fermentation to sorting of fermentable and non-fermentable solids can be processed at a relatively high dry matter content in a single vessel or similar device using free fall mixing for the mechanical processing of the waste fraction. 2. A process according to claim 1 , wherein the said fermentation claim 1 , or part of it is carried out in the same said vessel used for non-pressurised pre-treatment and enzymatic hydrolysis.3. A process according to claim 2 , wherein recovery of the resulting fermentation products is performed in the same said vessel used for non-pressurised pre-treatment claim 2 , enzymatic hydrolysis and fermentation by applying heat and/or vacuum to the vessel.4. A process according to any of or claim 2 , wherein a sorting/separation of the non-fermentable solids from the fermented mash is performed in the same said vessel used for non-pressurised pre-treatment claim 2 , enzymatic hydrolysis and fermentation.5. A process according to claim 3 , wherein said recovery in the same said vessel is only done from the solid part of the fermented mash after separation/removal of the liquid part.6. A process according to any of the to claim 3 , wherein the said mono- and/or polysaccharide containing waste fractions are derived from households such as e.g. unsorted municipal solid waste (MSW) claim 3 , MSW processed in some central sorting claim 3 , shredding or pulping device claim 3 , source sorted waste from households claim 3 , organic fractions and paper rich waste fractions claim 3 , RPF (Refuse-Derived-Fuel) fractions.7. A process according to any of the to claim 3 , wherein ...

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Номер записи: 13
10-02-2022 дата публикации

METASTABLE STATE MIXING

Номер: US20220041978A1
Автор: Church Jordan E., Everett Gabriel F.K., Greenwald Charles J., Laney Christopher J., Paloian Michael, Pruitt Judith G., Rosmarin Amanda K., Schuster Michael J.
Принадлежит:

Metastable state spore incubation mixing systems are described. An example system includes a spore container to store spores, a nutrient container, an arrangement of valves and tubes, a reciprocating pump, a mixing tube, and a holding tank. In a drawing phase of the system, a controller can control the reciprocating pump to draw a ratioed volume of the spores, the nutrients, and water through the valves and tubes. During an expelling phase of the system, the controller can control flow control valves to direct the spores, nutrients, and water through the mixing tube and into the holding tank. The controller can also direct a heater to heat the mixture in the holding tank to a predetermined temperature. Once the mixture reaches the temperature, the controller can also direct the system through a number of other phases of operation, including cooling and purging phases. 1. A method , comprising:drawing, via a reciprocating pump operated by a controller, a volume of a solution of spores from a spore container, a volume of a solution of nutrients from a nutrient container, and a volume of water through an arrangement of valves and tubes;expelling, via the reciprocating pump, the volume of the solution of spores, the volume of the solution of nutrients, and the volume of water from the reciprocating pump to a mixing tube;mixing, via the mixing tube, the volume of the solution of spores, the volume of the solution of nutrients, and the volume of water together into a mixture of the spores, the nutrients, and the water;providing the mixture of the spores, the nutrients, and the water to a holding tank from the mixing tube; andheating, via a heater operated by the controller, the mixture of the spores, the nutrients, and the water in the holding tank, wherein a thermocouple is used to measure a temperature of the mixture in the holding tank.2. The method of claim 1 , wherein the arrangement of valves and tubes comprises a number of flow control valves claim 1 , a number of ...

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Номер записи: 14
30-01-2020 дата публикации

Aseptic tissue processing method, kit and device

Номер: US20200032197A1
Автор: Nicola Kaye Price, Ryan Dominic Guest
Принадлежит: Immetacyte Ltd

The present invention concerns a single use aseptic kit comprising: a disaggregation module for receipt and processing of material comprising solid mammalian tissue; and a stabilisation module for storing disaggregated product material, wherein each of the modules comprises one or more flexible containers connected by one or more conduits adapted to enable flow of the tissue material there between; and wherein each of the modules comprises one or more ports to permit aseptic input of media and/or reagents into the one or more flexible containers. The invention further relates to an automated device for semi-automated aseptic disaggregation and/or enrichment and/or stabilisation of cells or cell aggregates from mammalian solid tissue comprising a programmable processor and the single use aseptic kit. The invention further relates to a semi-automatic aseptic tissue processing method.

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Номер записи: 15
08-02-2018 дата публикации

STEM CELL MANUFACTURING SYSTEM, STEM CELL INFORMATION MANAGEMENT SYSTEM, CELL TRANSPORT APPARATUS, AND STEM CELL FROZEN STORAGE APPARATUS

Номер: US20180035661A1
Автор: INABA Kiyonori, ODA Masaru, TANABE KOJI
Принадлежит:

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state. 1. A stem cell manufacturing system for manufacturing stem cells from somatic cells , the system comprising:one or more closed production device(s) configured to produce stem cells from somatic cells;one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells;one or more cryopreservation device(s) configured to cryopreserve the produced stem cells;a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state;a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; anda third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.2. The stem cell manufacturing system according to claim 1 , further comprising an entry memory device ...

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Номер записи: 16
11-02-2016 дата публикации

DEVICES, SYSTEMS AND METHODS FOR AUTOMATED TRANSFER OF A SAMPLE

Номер: US20160040114A1
Автор: Conway Kenneth Roger, Griffin Weston Blaine, Keskar Vandana, Loghin Evelina Roxana, Mao Ying, Smith Reginald Donovan, Zhang Chengkun, Zhang Zhipeng
Принадлежит:

A coupling device configured to form a sample access assembly is provided. The sample access assembly is configured to house a sample. The coupling device includes a heating component and a separating component. Further, the separating component is configured to separate portions of first and second containers that form first and second compartments of the sample access assembly. Moreover, the heating component is configured to heat at least a portion of the sample. 1. A coupling device configured to form a sample access assembly using first and second containers , wherein the sample access assembly comprises a first compartment and a second compartment , and wherein the sample access assembly is configured to house a sample , the coupling device comprising:a heating component configured to heat at least a portion of the sample; anda separating component configured to separate at least a portion of the first container and at least a portion of the second container from remaining portions of the first and second containers to form the first and second compartments of the sample access assembly,wherein the coupling device is configured to transfer at least a portion of the sample, and wherein the coupling device is configured to maintain a sterile environment for the sample at least during coupling of the first and second compartments.2. The coupling device of claim 1 , wherein the first and second compartments are respective portions of the first and second containers.3. The coupling device of claim 2 , further comprising:a first holder unit configured to receive at least a portion of the first container, wherein the first container is configured to receive the sample; anda second holder unit operatively coupled to the first holder unit, wherein the second holder unit is configured to receive at least a portion of the second container,wherein the first and second holder units comprise one or more degrees of freedom, and wherein the first and second holder units are ...

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Номер записи: 17
08-02-2018 дата публикации

PROCESS COMPRISING SULFUR DIOXIDE AND/OR SULFUROUS ACID PRETREATMENT AND ENZYMATIC HYDROLYSIS

Номер: US20180037862A1
Автор: Foody Brian, Foody Patrick J., MacDonald Daniel, Tolan Jeffrey S.
Принадлежит:

The present invention provides a process for producing a fermentation product from a lignocellulosic feedstock. The process comprises soaking a lignocellulosic feedstock in an aqueous solution to produce a soaked feedstock. The soaked feedstock is at least partially dewatered and the at least partially dewatered feedstock is subjected to pretreating in the presence of sulfur dioxide, sulfurous acid or a combination thereof to produce a pretreated feedstock composition. The pretreated feedstock composition is fed to an enzymatic hydrolysis in which the concentration of dissolved solids fed to the enzymatic hydrolysis is at least 50% (w/w) of the concentration of dissolved solids in the pretreated feedstock composition. The cellulose in the pretreated feedstock composition is hydrolyzed with cellulase enzymes in the presence of the dissolved solids to produce glucose. The glucose is fermented to produce the fermentation product. 1. A process for producing a fermentation product from a lignocellulosic feedstock comprising:(i) soaking the lignocellulosic feedstock in an aqueous solution to produce a soaked feedstock;(ii) at least partially dewatering the soaked feedstock to produce a partially dewatered feedstock;(iii) pretreating the partially dewatered feedstock in the presence of sulfur dioxide, sulfurous acid, or a combination thereof to produce a pretreated feedstock composition;(iv) feeding the pretreated feedstock composition to an enzymatic hydrolysis, wherein the concentration of dissolved solids in the pretreated feedstock composition fed to the enzymatic hydrolysis is at least about 50% of the concentration of dissolved solids in the pretreated feedstock composition produced in step (iii);(v) hydrolyzing the cellulose in the pretreated feedstock composition in the enzymatic hydrolysis with cellulose enzymes in the presence of said dissolved solids to produce glucose; and(vi) fermenting the glucose to produce the fermentation product.2. A process for producing ...

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Номер записи: 18
08-02-2018 дата публикации

SULFUR DIOXIDE AND/OR SULFUROUS ACID PRETREATMENT WITH SULFUR DIOXIDE RECOVERY

Номер: US20180037863A1
Автор: Dechman John, Foody Brian, Griffin Robert, Leduc Natacha, MacDonald Daniel G., Martens Kristin
Принадлежит:

A process for producing ethanol from lignocellulosic biomass includes adding at least one of sulfur dioxide and sulfurous acid to the lignocellulosic biomass to provide an equivalent sulfur dioxide loading of at least 10 wt % sulfur dioxide to dry lignocellulosic biomass. The acidified lignocellulosic biomass is pretreated at a temperature above about 185° C. and for a pretreatment time less than about 10 minutes, to provide a pretreated biomass composition wherein the biomass is readily hydrolyzed by enzymes. Advantageously, sulfur dioxide from at least one of the flash stream and a stream derived from the flash is recovered and recycled back into the process. 1. A process for producing ethanol from lignocellulosic biomass comprising:a) adding acid to lignocellulosic biomass to produce acidified lignocellulosic biomass, said acid comprising at least one of sulfur dioxide and sulfurous acid;b) pretreating said acidified lignocellulosic biomass to produce a pretreated biomass composition comprising cellulose, said pretreating conducted in a pressurized pretreatment reactor at a temperature above about 185° C. and at a pH less than about 1.5;c) reducing a pressure on the pretreated biomass composition to produce a flash stream and a cooled pretreated biomass composition;d) obtaining at least one of sulfur dioxide and sulfurous acid from at least one of the flash stream and a stream derived from the flash stream;e) hydrolyzing cellulose from the cooled pretreated biomass composition in the presence of a cellulase to produce glucose;f) fermenting at least a portion of the glucose to ethanol; andg) recycling the at least one of sulfur dioxide and sulfurous acid derived from step d) back into the process.2. The process according to claim 1 , wherein said acid is added in an amount equivalent to provide at least 12% weight sulfur dioxide on weight of dry lignocellulosic biomass.3. The process according to claim 1 , wherein said acid is added in an amount equivalent to ...

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Номер записи: 19
12-02-2015 дата публикации

Biomass processing system, saccharide solution production method using biomass feedstock, alcohol production method

Номер: US20150044729A1
Автор: Hideo Suzuki, Minoru Genta, Seiichi Terakura, Yoshio Kuromi, Yoshitaka Kimura
Принадлежит: Mitsubishi Heavy Industries Mechatronics Systems Ltd

A biomass processing system includes: a hydrolysis processing unit that decomposes, under a high-temperature/high-pressure condition, biomass feedstock in a processing tank having a gas-liquid interface, and removes a lignin component and a hemicellulose component; a biomass solid content discharge unit that discharges a biomass solid content 20 which is a hot water insoluble element; a slurrying vessel that subjects the discharged biomass solid content to slurrying; and a hot water discharge liquid introducing line L 2 that introduces, into the slurrying vessel 21 , a hot water discharge liquid 16 including a biomass hot-water soluble element.

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Номер записи: 20
18-02-2016 дата публикации

Processing biomass

Номер: US20160046965A1
Автор: Harrison Medoff, Marshall Medoff, Thomas Craig Masterman
Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation.

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Номер записи: 21
25-02-2016 дата публикации

System for delivery of microbial inoculants and related materials and methods

Номер: US20160053218A1
Автор: Brian B. Mcspadden Gardener, Bruce H. Caldwell
Принадлежит: 3bar Biologics Inc, Ohio State Innovation Foundation

Described herein are systems, methods and kits for culturing and applying fresh microbial inoculants in the field. The system, methods and kits are easy to use, reliable, sealed to prevent contamination, and can be stored on location, for use on demand by those unskilled in the microbiological arts. Particular embodiments described herein may be used to increase agricultural crop yields.

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Номер записи: 22
15-05-2014 дата публикации

Biomass liquefaction through gas fermentation

Номер: US20140134686A1
Автор: Jennifer Rosa Holmgren, Michael Anthony Schultz
Принадлежит: Lanzatech New Zealand Ltd

The invention provides methods and systems for the production of at least one product from the microbial fermentation of a gaseous susbtrate, wherein the gaseous substrate is derived from a biomass liquefaction process. The invention provides a method for improving efficiency of the fermentation by passing biomass accumulated in the fermentation process to the biomass liquefaction process for conversion to a gaseous substrate. In a particular aspect of the invention, the biomass liquefaction process is selected from pyrolysis or torrefaction.

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Номер записи: 23
01-03-2018 дата публикации

Antimicrobial susceptibility testing device, antimicrobial susceptibility testing kit, and antimicrobial susceptibility testing method

Номер: US20180057853A1
Автор: Chihiro Uematsu, Hideyuki Noda, Hiroko Tada, Masahiro Okanojo
Принадлежит: HITACHI LTD

Provided is a antimicrobial susceptibility testing device, including: an ATP examination culture plate that includes a reaction vessel, a reagent holding parts for holding reagents to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has plural layers that can be joined and separated; a gas feeding path for feeding a gas into the ATP examination culture plate; a heater; an optical detection unit; and a determination unit for determining sensitivity of a bacterial strain contained in the culture solution to a drug based on a detection result of the optical detection unit, wherein when the plural layers of the ATP examination culture plate are joined, at least the culture solution holding part and the reaction vessel are in a sealed state while communicating with each other.

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Номер записи: 24
04-03-2021 дата публикации

Method and an apparatus for treating plant based raw material with an enzymatic hydrolysis

Номер: US20210062231A1
Автор: Juha Tamper, Sami Turunen
Принадлежит: UPM Kymmene Oy

A method for treating plant based raw material with an enzymatic hydrolysis. The plant based raw material is treated to form lignocellulosic material. The lignocellulosic material or a solid fraction thereof is subjected to the enzymatic hydrolysis. The method includes treating the plant based raw material in at least one treatment stage for forming the lignocellulosic material including over 80% fine solid particles that are fiber-like or indefinable particles smaller than 0.2 mm and the viscosity of the lignocellulosic material is below 18000 mPas at 15% dry matter content. The method further includes subjecting the lignocellulosic material or at least one solid fraction thereof into the enzymatic hydrolysis for forming a lignin based material. The method further includes subjecting the lignin based material into at least one solid-liquid separation stage after the enzymatic hydrolysis and separating a lignin fraction and a soluble carbohydrate containing fraction.

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Номер записи: 25
22-05-2014 дата публикации

Methods for Collection, Storage and Transportation of Biological Specimens

Номер: US20140141411A1
Автор: Darrell A. Burns, Joe T. Huong, Robert M. Lloyd, JR.
Принадлежит: Vivebio LLC

The present invention provides methods for collecting, storing or transporting liquid suspension of biological specimens containing analytes of interest in a dry state. The dried biological specimens containing analytes of interest are reconstituted and released for subsequent analysis by compressing or centrifuging the matrix. Also provided are method of using kits for collecting, storing, transporting and recovering biological specimens containing analytes of interest.

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Номер записи: 26
17-03-2016 дата публикации

SYSTEMS, DEVICES AND METHODS FOR AGRICULTURAL PRODUCT PULPING

Номер: US20160076198A1
Автор: Charlton William Robert Charles, Christensen Kurt, Goodfellow Neill
Принадлежит:

This disclosure relates to systems, devices and methods for pulping agricultural products. Features for ensiling agricultural crops, separating solids and liquids, and processing the solids and liquids for use in a variety of products and processes are disclosed. For instance, systems and methods are disclosed for pulping grass crops, making products for energy conversion processes, screening fine debris, cell bursting, using strongly alkaline chemicals in pulping processes, and using sorghum plant for producing pulp. 1. An ensilage system for pulping agricultural crop , the system comprising:an ensilage facility configured to store the crop;a separation stage coupled with the ensilage facility, the separation stage configured to receive the crop from the ensilage facility and to separate solids from liquids in the crop;a liquids pathway coupled with the separation stage and configured to receive the liquids separated in the separation stage and to process the liquids for energy production; anda solids pathway coupled with the separation stage and configured to receive the solids separated in the separation stage and to process the solids into pulping product.2. The system of claim 1 , wherein the ensilage facility is configured for storing the crop long term for pulping production.3. The system of claim 1 , wherein the ensilage facility is configured for storing wet crop.4. The system of claim 1 , wherein the ensilage facility is further configured to compact the crop.5. The system of claim 1 , wherein the ensilage facility is further configured to enable long term storage without significant biological decay of organic compounds in the crop to exposure to oxygen while increasing sugar availability for purpose of energy production.6. The system of claim 1 , wherein the ensilage facility is further configured to enable long term storage of crop without exposure to molds and fungus.7. The system of claim 1 , wherein the crop is grass crop.8. The system of claim 1 , ...

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Номер записи: 27
05-03-2020 дата публикации

ADSORBENT FOR ANAEROBIC DIGESTION PROCESSES

Номер: US20200071218A1
Автор: Free Andrew, Mumme Jan, Pietrzyk Julian, Srocke Franziska
Принадлежит:

A particulate carbon adsorbent for use in anaerobic digestion is provided. The particulate carbon adsorbent is substantially planar and comprising between 40-90 wt % carbon. Methods of manufacture of the particulate carbon adsorbent are also provided. 1. A particulate carbon adsorbent for use in anaerobic digestion , the particulate carbon adsorbent being substantially planar and comprising between 40-90 wt % carbon.2. The particulate carbon adsorbent for use in anaerobic digestion according to formed by pyrolysis of substantially planar organic material.3. The particulate carbon adsorbent for use in anaerobic digestion according to claim 2 , wherein the substantially planar organic material comprises paper or cardboard or similar.4. The particulate carbon adsorbent for use in anaerobic digestion according to claim 2 , wherein the substantially planar organic material comprises a plurality of layers of substantially planar organic material.5. The particulate carbon adsorbent for use in anaerobic digestion according to claim 2 , wherein the substantially planar organic material is a composite material.6. The particulate carbon adsorbent for use in anaerobic digestion according to claim 5 , wherein the composite material is formed from layers of paper and layers of plastic and/or wax and/or aluminium.7. The particulate carbon adsorbent for use in anaerobic digestion according to claim 1 , wherein the anaerobic digestion is carried out in a bioreactor claim 1 , and as a result claim 1 , the particulate carbon adsorbent is used in a bioreactor.8. The particulate carbon adsorbent for use in anaerobic digestion according to claim 1 , wherein the particulate carbon adsorbent provides a substrate for the formation of an active biofilm.9. The particulate carbon adsorbent for use in anaerobic digestion according to claim 1 , wherein the particulate carbon adsorbent comprises an active biofilm.10. The particulate carbon adsorbent for use in anaerobic digestion according to ...

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Номер записи: 28
24-03-2016 дата публикации

Systems and methods for processing cells

Номер: US20160083691A1
Автор: John Meade, Rouzbeh R. Taghizadeh
Принадлежит: Auxocell Laboratories Inc

The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.

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Номер записи: 29
25-03-2021 дата публикации

ANAEROBIC DIGESTER

Номер: US20210087519A1
Автор: KANU Ifeyinwa Rita
Принадлежит:

Anaerobic digestion apparatus comprises a first chamber for retaining organic matter before and/or during anaerobic digestion and a second chamber for retaining organic matter during anaerobic digestion. The anaerobic digestion apparatus is configured to refrigerate or heat the first chamber to suppress methanogenesis in the first chamber. The anaerobic digestion apparatus comprises a controller programmed to regulate the anaerobic digestion process and to thereby reduce system perturbations. The flow of organic matter to the second chamber where methanogenesis is regulated. There is disclosed an inoculum for anaerobic digestion comprising and 147-. (canceled)48. A method of generating methane from organic matter , the method comprising:exposing the organic matter to acetogenic microorganisms; andsubsequently exposing at least a portion of the organic matter to methanogenic microorganisms.491. The method of claim , wherein exposing the organic matter to acetogenic microorganisms occurs at a temperature equal to or below 10° C.501. The method of claim , wherein exposing the at least a portion of the organic matter to methanogenic microorganisms occurs at a temperature greater than 10° C.511. The method of claim , wherein exposing the organic matter to acetogenic microorganisms occurs at a temperature equal to or below 10° C.; and exposing at least a portion of the organic matter to methanogenic microorganisms occurs at a temperature greater than 10° C.521AcetobacteriumMethanosaeta.. The method of claim wherein the acetogenic microorganisms belong to the genus , and the methanogenic microorganisms belong to the genus535Acetobacterium woodiiMethanosaeta concilii.. The method of claim , wherein the acetogenic bacteria belong to the species and the methanogenic archaea belong to the species541AcetobacteriumMethanosaeta.. The method of claim wherein the acetogenic microorganisms consist essentially of microorganisms belonging to the genus , and the methanogenic ...

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Номер записи: 30
25-03-2021 дата публикации

ANAEROBIC DIGESTER

Номер: US20210087520A1
Автор: KANU Ifeyinwa Rita
Принадлежит:

Anaerobic digestion apparatus comprises a first chamber for retaining organic matter before and/or during anaerobic digestion and a second chamber for retaining organic matter during anaerobic digestion. The anaerobic digestion apparatus is configured to refrigerate or heat the first chamber to suppress methanogenesis in the first chamber. The anaerobic digestion apparatus comprises a controller programmed to regulate the anaerobic digestion process and to thereby reduce system perturbations. The flow of organic matter to the second chamber where methanogenesis is regulated. There is disclosed an inoculum for anaerobic digestion comprising and 147-. (canceled)48. A method of hydrolyzing organic matter , the method comprising:determining a parameter indicative of the amount of protein in the organic matter; andexposing the organic matter to an amount of one or more hydrolytic enzymes,wherein the amount of hydrolytic enzymes to which the organic matter is exposed is dependent on the value of the determined parameter.491. The method of claim , wherein the amount of one or more hydrolytic enzymes is in proportion to the value of the determined parameter.501. The method of claim , wherein the method comprises estimating the protein concentration of the organic matter based on the value of the determined parameter.513. The method of claim , wherein the determined parameter is the total nitrogen concentration of the organic matter.523. The method of claim , further comprising estimating a carbohydrate concentration and a lipid concentration of the organic matter based on the estimated protein concentration , and wherein the amount of hydrolytic enzymes to which the organic matter is exposed is based on the estimated protein concentration , carbohydrate concentration , and lipid concentration.531. The method of claim , wherein the organic matter is exposed to the amount of one or more hydrolytic enzymes in an anaerobic digestion apparatus comprising:a first chamber for ...

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Номер записи: 31
30-03-2017 дата публикации

SYSTEM AND METHOD FOR THERMOPHILIC ANAEROBIC DIGESTER PROCESS

Номер: US20170088803A1
Автор: Knoop Robert
Принадлежит:

An anaerobic digestion system is provided that includes a blend tank operable to control and perform pre-treatment of feedstock. An anaerobic digester is operable to digest the feedstock provided from the blend tank in a totally enclosed oxygen-free environment within a specific temperature range. A bio-mass tank processes liquid digestate from the anaerobic digester. One or more baffles are positioned in the digester, with the one or more baffles providing for plug flow through at least a portion of the digester to create baffled zones that are at least partially operable independently of adjacent baffled zones. A bio-mass tank processes liquid digestate from the anaerobic digester. An energy source is coupled to the anaerobic digester. 1. An anaerobic digestion system , comprising:a blend tank operable to control and perform pre-treatment of feedstock;an anaerobic digester operable to digest the feedstock provided from the blend tank in a totally enclosed oxygen-free environment within a specific temperature range;a bio mass tank operable to process liquid digestate from the anaerobic digester;one or more baffles positioned in the digester, the one or more baffles providing for plug flow through at least a portion of the digester and create baffled zones that are at least partially operable independently of adjacent baffled zones; 'an energy source coupled to the anaerobic digester.', 'a bio mass tank operable to process liquid digestate from the anaerobic digester; and'}2. The system of claim 1 , wherein the energy source is an electromagnetic energy source.3. The system of claim 1 , wherein the energy source is a microwave source.4. The system of claim 3 , wherein the microwave source produces microwave radiation that is applied to solubilize a lignocellulose material.5. The system of the application of the microwave radiation releases high lipid contents.6. The system of claim 5 , in response to the release of high lipid contents there is a transfer of one or ...

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Номер записи: 32
12-05-2022 дата публикации

CELL TREATMENTS AND THERAPEUTIC REINFUSION METHODS

Номер: US20220143082A1
Автор: Alamin Todd, Edidin Avram Allan, Malandain Hugues, Montague-Alamin Healey
Принадлежит:

Tumor tissue, including soft and/or bony tissue, are harvested from a subject and morcellated. The morcellated tissue is placed in a cartridge which is placed in a containment chamber of a tumor tissue processing device. Cancer cells in the morcellated tumor tissue are destroyed without destroying tumor antigens therein. These cells are destroyed cryogenically by exposing the cartridge to a cooling fluid such as liquid nitrogen, optionally with a warming cycle, and optionally with more than one freezing/thawing cycle. The treated tissue and/or cells are then extracted from the cartridge and reintroduced to the subject after they have reached a threshold condition. The treated tissue and/or cells can be reintroduced via a containment sleeve or a reimplantation bag. 1. A method for treating cancer , the method comprising:reintroducing into a subject tumor tissue harvested from the subject, the reintroduced tumor tissue having had cancer cells thereof devitalized without destroying tumor antigens in said cancer cells.2. The method of claim 1 , wherein the tumor tissue harvested from the subject comprises one or more of soft tissue or bony tissue.3. The method of claim 1 , further comprising harvesting the tumor tissue from the subject.4. The method of claim 1 , further comprising morcellating the tumor tissue harvested from the subject prior to reintroducing the tumor tissue into the subject.5. The method of claim 4 , wherein morcellating the tumor tissue comprises placing the harvested tumor tissue in a containment chamber claim 4 , wherein the tumor tissue is morcellated in the containment chamber.6. The method of claim 1 , further comprising devitalizing cancer cells in the tumor tissue harvested from the subject.7. The method of claim 6 , wherein devitalizing cancer cells in the tumor tissue comprises destroying the cancer cells.8. The method of claim 6 , wherein devitalizing cancer cells in the tumor tissue comprises cooling the tumor tissue.9. The method of claim ...

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Номер записи: 33
28-03-2019 дата публикации

METHOD FOR THE CONTINOUS THERMAL HYDROLYSIS OF ORGANIC MATERIAL AND A PLANT SUITABLE FOR IMPLEMENTING SUCH METHOD

Номер: US20190092669A1
Автор: ARAUZO PEREZ Ivan, PERMUY VILA Juan
Принадлежит: AQUATEC, PROYECTOS PARA EL SECTOR DEL AGUA, S.A.U.

A method for the continuous thermal hydrolysis of organic material, which includes at least the stages of: a) pressurizing starting organic material at a pressure setpoint P1; b) inline mixing organic material with steam to obtain a mixture at a controlled temperature setpoint T1, selected below the steam saturation temperature of the mixture; c) continuously introducing the mixture obtained, which is in a liquid phase, into a hydrolysis reactor; d) continuously extracting hydrolyzed mixture from the hydrolysis reactor; and e) suddenly depressurizing the hydrolyzed mixture separating a liquid fraction from a steam fraction, which is recovered. The steam employed to produce the mixture in stage b) includes steam recovered in operation e) which is recompressed. 23. The method according to claim 1 , characterized in that the starting organic material () is at room temperature.39. The method according to claim 1 , characterized in that the working pressure of the hydrolysis reactor () is kept above the steam saturation pressure at the temperature at which the process is carried out.414. The method according to claim 1 , characterized in that the recovered steam () is recompressed employing steam recompressing methods either mechanical claim 1 , thermal or a combination of both.5781714. The method according to claim 1 , characterized in that the steam () employed for producing the mixture () in step b) is obtained by mixing new steam () with steam () recovered from stage e) which is recompressed.6141817. The method according to claim 4 , characterized in that the recovered steam () is recompressed in a first step claim 4 , by a steam compressor claim 4 , which rises the pressure thereof between 4 and 6 bar; and subsequently in a second step the mechanically recompressed steam () is thermally compressed by mixing it with new steam () claim 4 , the differential pressure provided in this second step being between 1 and 1.5 bar.7. The method according to claim 5 , ...

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Номер записи: 34
12-05-2022 дата публикации

Method for cleaning a reactor for processing a lignocellulosic biomass

Номер: US20220145232A1
Автор: Caroline Aymard, Meriem Bouras, Olivier Carnnot
Принадлежит: Agro Industrie Recherches et Developpements ARD, IFP Energies Nouvelles IFPEN, Institut National de Recherche pour lAgriculture lAlimentation et lEnvironnement

The present invention relates to a processing method at acidic or neutral pH in a reactor ( 4 ) for processing lignocellulosic biomass (P), said process including a continuous cleaning phase of the reactor which comprises introducing a basic aqueous solution (EB) into said reactor containing the biomass being processed.

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Номер записи: 35
12-05-2022 дата публикации

Closed Tissue Disaggregation and Cryopreservation

Номер: US20220145234A1
Автор: LAMB Stephen, Morris George John
Принадлежит:

Disclosed is a device () for the disaggregation of tissue samples into individual cells or cell clumps in a closed flexible tissue sample bag (); the device including two or more resilient feet () which tread sequentially a tissue sample bag receiving area (). Also disclosed is a heat transfer plate () for transferring heat energy to or from the area (), the plate having one plate surface () adjacent the area () and an opposing surface () exposed to external thermal influence which faces away from the area (). Further disclosed is a tissue sample receiving bag () comprising one or more flexible plastics cavity () formed from two layers of the plastics sealed around their edges to form a generally rectilinear periphery with the cavity or cavities () within the periphery, and at one side of the periphery is formed one or more sealable access ports (). One part of the bag is left unsealed to provide a tissue sample receiving opening. 1. A device for the disaggregation of tissue samples into individual cells or cell clumps in a closed flexible bag , the device including a mechanical disaggregation mechanism and a tissue sample bag receiving area , said device further including a heat transfer plate for transferring heat energy to or from the area , the plate having a first plate surface adjacent the area and an opposing surface exposed to external thermal influence which faces away from the area the disaggregation mechanism including plural treading feet each urged toward the first plate surface with generally linear motion only by force from a respective resilient member , and each foot being further movable away from the first plate under influence of a mechanical member which is arranged also to compress said respective resilient member during said movement away from the first plate.2. The device as claimed in claim 1 , wherein the mechanism includes two or more feet arranged to tread sequentially the tissue sample bag receiving area.3. The device as claimed in claim ...

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Номер записи: 36
12-04-2018 дата публикации

Cell Suspension Preparation Technique and Device

Номер: US20180098840A1
Автор: Fiona M. Wood, Marie L. Stoner
Принадлежит: Avita Medical Inc, Avita Medical Ltd

The present invention provides for methods and devices suitable for producing a transplantable cellular suspension of living tissue suitable for grafting to a patient. In applying the method and/or in using the device, donor tissue is harvested, subjected to a cell dissociation treatment, cells suitable for grafting back to a patient are collected and dispersed in a solution that is suitable for immediate dispersion over the recipient graft site.

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Номер записи: 37
12-04-2018 дата публикации

Novel Cryopreservation Bags and method of use thereof for Closed System, High Capacity Cell-Banking

Номер: US20180100135A1
Автор: Coupier Hervé François
Принадлежит: MERIAL INC.

The disclosure provides a cell freezing and storage bag assembly and a method for using the assembly in banking eukaryotic cells for later seed train expansion. The bag is constructed principally of fluorinated ethylene propylene (FEP) fabric, and is designed to be filled such that the cell suspension has a very thin cross-section. The bag design includes at least an inlet conduit and an outlet or inoculation conduit, which can be sterilely welded to the source of the eukaryotic cells. The use of at least two sterile-weldable conduits allows for “closed system” filling of the bags, which significantly reduces the risk of contamination relative to other cell-banking methods. The bag also include a sleeve, which can be thermo-welded to form an enclosure, which protects the inlet and outlet conduits against contamination and mechanical damage during freezing, storage and subsequent thawing. In the method, once each bag is filled, its corresponding inlet conduit is sealed, and both the inlet and outlet conduits are enclosed within the bag's sleeve. This closed system method obviates the need for sterile environments (e.g. laminar flow unit). 114-. (canceled)15. A high-capacity cryopreservation bag , which is adapted for use in a high capacity biological material , closed cryopreservation banking system , and which is resistant to liquid nitrogen temperatures comprising:a. a first fluorinated ethylene propylene (FEP) enclosure;b. at least two sterile-weldable fluid conduits adapted for use in a high capacity biological material, closed banking system;c. a first rigid FEP inlet conduit, in fluid connection with the first FEP enclosure, and connectable to the first of the two sterile-weldable fluid conduits, wherein said first conduit is a first flexible PVC tube;d. a second rigid FEP outlet conduit, in fluid connection with the first FEP enclosure, and connectable to the second of the two sterile-weldable fluid conduits, wherein said second conduit is a second flexible ...

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Номер записи: 38
21-04-2016 дата публикации

SACCHARIFICATION REACTION APPARATUS

Номер: US20160108355A1
Автор: Izumi Noriaki, Kusuda Hiromasa, Nishino Takashi, Tajiri Hironori, TSUZAWA Masaki
Принадлежит: KAWASAKI JUKOGYO KABUSHIKI KAISHA

A saccharification-reaction apparatus includes: a reactor which causes a saccharification-reaction of a raw material; and a raw material charging device which charges the raw material into the reactor at a predetermined interval; the reactor includes: a heating steam feeder which increases a temperature of the raw material charged from the charging device to a saccharification-reaction temperature; and a feeding mechanism which sequentially feeds each of the charged raw materials toward an outlet-port in a predetermined short period of time while causing the saccharification-reaction of each raw material under a high temperature and pressure; the raw material charging-device charges into the reactor, the raw material with a suitable bulk density for a saccharification-reaction speed of the reactor; in this manner, the saccharification-reaction apparatus charges the raw material in a hydrolytic saccharification state into the reactor by the raw material charging device; and hydrolytically saccharifying the raw material efficiently in the reactor. 1. A saccharification reaction apparatus comprising:a reactor configured to cause a saccharification reaction of a raw material; anda raw material charging device configured to charge the raw material into the reactor at a predetermined interval, whereinthe raw material charging device is configured to charge, into the reactor, the raw material with such a bulk density that the raw material breaks into pieces and is dispersed when charged into the reactor, and a heating steam feeder configured to increase a temperature of the raw material charged from the raw material charging device to a saccharification reaction temperature; and', 'a feeding mechanism configured to discharge the charged raw material through an outlet port after the saccharification reaction of the raw material has been caused under a high temperature and a high pressure for a predetermined time., 'the reactor includes2. The saccharification reaction ...

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Номер записи: 39
13-05-2021 дата публикации

METHOD AND DEVICE FOR TREATING BIOMASS

Номер: US20210140105A1
Автор: AVERHEIM Andreas, LAMBERT Francois
Принадлежит:

The invention relates to a method for treating biomass (). Biomass () is fed to a pressurized prehydrolysis reactor unit () by means of a feeding system (), wherein by means of the feeding system () the biomass () is compressed. A filtrate is squeezed out of the biomass () by means of the feeding system (), in particular by a first plug screw () or a second plug screw () of the feeding system (). The biomass () is then thermally treated in the pressurized prehydrolysis reactor unit (), discharged from the pressurized prehydrolysis reactor unit () afterwards, diluted with the filtrate before or after the discharge, and treated with an enzyme subsequently. 1. A method for treating biomass , the method comprising:feeding biomass to a pressurized prehydrolysis reactor unit by means of a feeding system, wherein by means of the feeding system the biomass is compressed, and wherein a filtrate is squeezed out of the biomass by means of the feeding system;separating the filtrate from the biomass;thermally treating the biomass in the pressurized prehydrolysis reactor unit;discharging the thermally treated biomass from the pressurized prehydrolysis reactor unit; andtreating the discharged and diluted biomass with an enzyme;wherein the biomass is diluted with the filtrate before or after being discharged from the pressurized prehydrolysis reactor unit.2. The method of claim 1 , wherein the thermally treated biomass is discharged from the pressurized prehydrolysis reactor through a steam explosion.3. The method of claim 1 , wherein the thermally treated biomass is diluted before it is discharged from the pressurized prehydrolysis reactor unit.4. The method of claim 3 , wherein the biomass has a temperature below 100° C. during discharging and no flashing occurs during discharging.5. The method of claim 3 , wherein the biomass has a temperature above 100° C. during discharging and a flashing without a steam explosion occurs during discharging.6. The method of claim 3 , wherein ...

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Номер записи: 40
05-05-2016 дата публикации

Palette-based systems for analyte characterization

Номер: US20160121326A1
Автор: Arkadij M. Elizarov, Artem Y. Lebedev
Принадлежит: Trace-Ability Inc

Systems, devices, and methods are provided that facilitate synthesis and analysis of chemical compounds on a convenient and automatable palette-based platform. Palettes utilized for analysis support a variety of functional sites, including test wells, separation devices, and test sites that have rapid thermal equilibration, on a single test fixture. This permits both the performance of a wide variety of tests and of tests with different temperature requirements or responses in parallel, on the same test fixture. Such systems, devices, and methods are particularly suitable for production and characterization of radiopharmaceuticals.

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Номер записи: 41
13-05-2021 дата публикации

INTESTINAL FLORA EXTRACTION VESSEL

Номер: US20210140858A1
Автор: FUJIMOTO Takashi, Nabeshima Akihiro, Nagata Yasushi, Nishioka Masaki, Takiguchi Osamu
Принадлежит: OTSUKA PHARMACEUTICAL FACTORY, INC.

The present invention provides an intestinal flora extraction vessel for extracting an intestinal flora from feces, the vessel including: a vessel main body with a first opening portion and a second opening portion formed therein, the vessel main body being configured so as to be deformable; an opening/closing mechanism configured to open/close the first opening portion of the vessel main body; a filter member accommodated in the vessel main body and partitioning the vessel main body into a first region located on the side of the first opening portion and a second region located on the side of the second opening portion, the filter member being configured to filter off the intestinal flora from the feces; and a communication member attached to the second opening portion, and configured to allow the second region to be in communication with the outside of the vessel main body. 1. An intestinal flora extraction vessel for extracting an intestinal flora from feces , the vessel comprising:a vessel main body with a first opening portion and at least one second opening portion formed therein, the vessel main body being configured so as to be deformable;an opening/closing mechanism configured to open/close the first opening portion of the vessel main body;a filter member accommodated in the vessel main body and partitioning the vessel main body into a first region located on the side of the first opening portion and a second region located on the side of the second opening portion, the filter member being configured to filter off the intestinal flora from the feces; anda communication member attached to the second opening portion, and configured to allow the second region to be in communication with the outside of the vessel main body.2. The intestinal flora extraction vessel according to claim 1 , wherein the filter member is formed in a bag shape having an internal space open to the side of the first opening portion.3. The intestinal flora extraction vessel according to ...

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Номер записи: 42
07-05-2015 дата публикации

SUBSTRATE UNIT, PRESERVATION DEVICE AND METHOD FOR THE CRYOPRESERVATION OF A BIOLOGICAL SAMPLE

Номер: US20150125954A1
Автор: Beier Axel, FUHR Guenter R., NEUBAUER Julia, Zimmermann Heiko
Принадлежит:

The invention relates to a substrate unit (), in particular for the cryopreservation of a biological sample () containing biological cells (), comprising a substrate platform () having a front side and a back side, wherein the front side of the substrate platform () has a cultivation surface () for receiving the biological sample (), a first chamber (), which comprises the cultivation surface () of the substrate platform (), wherein the first chamber () is configured for receiving a cultivation liquid (), a second chamber (), which is configured for receiving a temperature control medium (), and a chamber bracket () for receiving the substrate unit () in a cryopreservation device in a pivotable manner, wherein the first chamber () and the second chamber () are connected to each other in an adjacent manner, and the substrate platform () forms a separating wall between the first chamber () and the second chamber (), and wherein the back side of the substrate platform () faces the second chamber (). The invention further relates to a cryopreservation device (), in which the substrate unit () is arranged in rotatable manner, and a method for the cryopreservation of a biological sample (). 1. Substrate device for the cryopreservation of a biological sample including biological cells , comprisinga substrate platform having a front side and a back side, the front side of the substrate platform forming a cultivation surface for receiving the biological sample, anda first chamber, in which the cultivation surface of the substrate platform is included, the first chamber being adapted for receiving a cultivation liquid,a second chamber, which is adapted for receiving a temperature control medium, anda chamber holder, which is adapted for receiving the substrate device in a cryopreservation apparatus in a pivotable manner, whereinthe first chamber and the second chamber are connected to each other in an adjacent manner, andthe substrate platform forms a separating wall between ...

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Номер записи: 43
25-08-2022 дата публикации

Cell preservation or transportation instrument and cell transporation method

Номер: US20220267704A1
Автор: Izumi OGURA, Keiji Shigesada, Nao Yamazaki, Shinji MIMA, Shun GOTO, Yuki Imakura
Принадлежит: Fujifilm Corp

An object of the present invention is to provide a transportation instrument and a transportation method that enable transportation by using a cell culture container as it is. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable supply of oxygen required for respiration of cells. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable suppression of migration of a culture medium into and out of cells caused by the influence of an increase in internal pressure with respect to the temperature during transportation. According to the present invention, provided is a cell preservation or transportation instrument including a plurality of cell storage containers, a plate which has a plurality of recesses for holding the plurality of cell storage containers therein, and a flexible material sheet which seals upper opening portions of the plurality of cell storage containers and upper side wall portions formed by side walls of the recesses of the plate and allows ventilation between an inside and an outside of the plurality of cell storage containers.

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Номер записи: 44
16-04-2020 дата публикации

ANAEROBIC DIGESTER

Номер: US20200115670A1
Автор: KANU Ifeyinwa Rita
Принадлежит:

Anaerobic digestion apparatus comprises a first chamber for retaining organic matter before and/or during anaerobic digestion and a second chamber for retaining organic matter during anaerobic digestion. The anaerobic digestion apparatus is configured to refrigerate or heat the first chamber to suppress methanogenesis in the first chamber. The anaerobic digestion apparatus comprises a controller programmed to regulate the anaerobic digestion process and to thereby reduce system perturbations. The flow of organic matter to the second chamber where methanogenesis is regulated. There is disclosed an inoculum for anaerobic digestion comprising and 147-. (canceled)48. Anaerobic digestion apparatus comprising a first chamber for retaining organic matter before and/or during anaerobic digestion and a second chamber for retaining organic matter during anaerobic digestion , the anaerobic digestion apparatus being configured to refrigerate the first chamber to thereby suppress methanogenesis in the first chamber , wherein the apparatus is configured to refrigerate the first chamber to a temperature equal to or below 10° C. and to heat the second chamber to a temperature greater than 10° C.49. The anaerobic digestion apparatus according to claim 48 , further comprising one or more sensors configured to measure one or more parameters indicative of volume claim 48 , mass claim 48 , composition claim 48 , protein concentration claim 48 , concentration of nitrogen-containing species claim 48 , nitrogen concentration claim 48 , carbohydrate concentration claim 48 , lipid concentration claim 48 , fatty acid concentration claim 48 , and/or pH of material retained within the first and/or second chambers.50. The anaerobic digestion apparatus according to claim 49 , further comprising a buffer chamber connected to the first and second chambers claim 49 , through which organic matter is moved from the first to the second chamber claim 49 , wherein the apparatus is configured to ...

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Номер записи: 45
14-05-2015 дата публикации

PACKAGE FOR CELL-CONTAINING MATERIAL AND PACKAGE CONTAINING CELL-CONTAINING MATERIAL

Номер: US20150132842A1
Автор: Sugiura Makoto
Принадлежит:

A package for cell-containing material includes an inner and outer bag. The periphery of the inner bag except an opening is sealed. The periphery of the outer bag except an opening is sealed. The outer bag contains the inner bag so the opening faces outward. A portion of the opening outside the inner bag is sealed and forms an outer bag sealed portion. A portion of the opening that overlaps the inner bag is sealed and forms an inter-bag sealed portion. A sterilized closed space may be surrounded by the outer surface of the inner bag and the inner surface of the outer bag. An adhesive strength of heat sealing of the inner surfaces of the opening of the inner bag may be greater than an adhesive strength of the inter-bag sealed portion and an adhesive strength of the outer bag sealed portion. 1. A package for cell-containing material configured to contain cell-containing material , comprising:an inner bag having an inlet for introducing the cell-containing material into the inner bag;an outer bag that contains the inner bag such that the inlet faces outward, the outer bag being sealed at the periphery thereof, except a portion in contact with the inner bag;an inter-bag sealed portion at which an outer surface of the inlet of the inner bag is bonded to an inner surface of the outer bag in contact with the outer surface of the inlet of the inner bag; anda sterile closed space surrounded by an outer surface of the inner bag and the inner surface of the outer bag.2. The package for cell-containing material according to claim 1 , whereinthe inlet is an opening through which the cell-containing material can be delivered into the inner bag, and the inner bag is formed of such a material that the adhesive strength of heat sealing of the opening is greater than the adhesive strength of the inter-bag sealed portion.3. A package containing cell-containing material claim 1 , comprising:{'claim-ref': {'@idref': 'CLM-00002', 'claim 2'}, 'a package for cell-containing material ...

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Номер записи: 46
21-05-2015 дата публикации

METHOD OF FREEZING CELLS

Номер: US20150140656A1
Автор: Cruz Pacheco Antonio, Gallot Escobal Natalia, Hernan Izquierdo Roberto
Принадлежит:

The present invention is a method of freezing cells comprising the steps of incubating said cells in a solution comprising a cryoprotective agent, concentrating the cells resulting from the previous step withdrawing the eluent essentially free of cells, and freezing the resulting concentrated cells. The cells frozen by the invention's method render a high post-thawing viability, reduce cryoprotectant related toxic events and promote cells life in a suspension state after thawing. The invention also comprises the container comprising the frozen cells. 1. A method of freezing cells , comprising:a) incubating said cells in a solution, said solution comprising a cryoprotective agent,b) concentrating the cells resulting from step a) and withdrawing an eluent essentially free of cells resulting thereof, andc) freezing the concentrated cells resulting from step b).2. The method according to claim 1 , wherein said incubating step a) is performed at a temperature of 3 to 5° C.3. The method according to claim 1 , wherein said cryoprotective agent is selected from the group consisting of dimethyl sulphoxide claim 1 , glycerol claim 1 , polyvinyl-pyrrolidone claim 1 , ethylene glycol claim 1 , methanol claim 1 , methyl acetamide and sugars claim 1 , and combinations thereof.4. The method according to claim 3 , wherein said cryoprotective agent is dimethyl sulphoxide.5. The method according to claim 1 , wherein said concentrating step b) is performed at a temperature cooling gradient of 1 to 2° C./min.6. The method according to claim 1 , wherein said concentrating step b) is performed by centrifugation or filtration.7. The method according to claim 1 , wherein said freezing step c) is performed at a temperature cooling gradient of 1 to 2° C./min.8. The method according to claim 1 , wherein said freezing step c) is performed by vitrification.9. The method according to claim 1 , wherein said freezing step c) is performed in a receptacle of a surface-to-volume ratio between 6 and ...

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Номер записи: 47
07-08-2014 дата публикации

Cold Box with a Rack Loaded with Tube-Shaped Vessels for Automated Filling by an Automated Pipetting Device

Номер: US20140216059A1
Автор: Kiehntopf Michael, Moore Thomas
Принадлежит: CYBIO AG

A cold box having a cooling receptacle filled with a dry protective gas in which is accommodated a rack loaded with tube-shaped vessels. The cooling receptacle is covered by a displaceable lid which is associated with a lid part and which is preferably formed by an outer lid and a rotatable inner lid integrated in the outer lid so that the cooling receptacle is completely covered in every position of the lid. At least one of the through-holes provided in the inner lid can be arranged over a respective tube-shaped vessel by a coordinated displacement of the outer lid and rotation of the inner lid so that the tube-shaped vessel located under the through-hole in alignment therewith can be filled with a sample through the through-hole by means of a commercially available pipette tip/dispensing needle of an automated pipetting device. 1. A cold box having a rack loaded with tube-shaped vessels for automated filling by an automated pipetting device , comprising a bottom part and a lid part , a rack which is adapted to be loaded with tube-shaped vessels in a matrix of rows and columns , wherein said rows extend along the width of the rack and said columns extend along the length of the rack , a cooling receptacle enclosed by thermal insulation at least partially arranged in the bottom part and adapted to the outer dimensions of the loaded rack , and in which the loaded rack is placed , said lid part having a lid frame and a lid , said lid being is displaceable in the lid frame in a direction of the columns or rows of the rack and in which is provided a quantity of openings which are adapted to the circumference size of a commercially available pipette tip/dispensing needle of an automated pipetting device so that one of the openings can be positioned over each individual tube-shaped vessel in the rack , said lid completely covering the cooling receptacle in every position so that a dry protective gas with which the cooling receptacle is filled and which is heavier than air ...

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Номер записи: 48
17-05-2018 дата публикации

SYSTEM FOR HYPOTHERMIC TRANSPORT OF SAMPLES

Номер: US20180132478A1
Автор: Anderson Lisa Maria, Judson Jared Alden
Принадлежит:

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature. 1. A system for transporting a biological sample , comprising: a pumping chamber having a semi-permeable membrane configured to push against a fluid and cause the fluid to circulate inside the first transport container;', 'a sample storage chamber, configured to receive a biological sample, and configured to interface with and be in fluidic communication with the pumping chamber;', 'a fill port providing a fluid path between the exterior of the first transport container and the sample storage chamber; and', 'a vent port providing a fluid path between the exterior of the first transport container and the pumping chamber; and, 'a first transport container comprisinga second transport container comprising an insulated cavity for receiving the first transport container and having recesses for receiving cooling media.2. The system of claim 1 , wherein the semi-permeable membrane is disposed at an inclined angle with respect to horizontal when the first transport container is placed on a horizontal surface.3. The system of claim 2 , wherein the semi-permeable membrane is inclined at an angle between approximately 1°-30° with respect to horizontal.4. The system of claim 1 , further comprising a valve in communication with the sample storage container and the pumping chamber.5. The system of claim 4 , wherein the valve is a ball check valve configured to allow fluid flow from the sample storage chamber to pumping chamber.6. The system of claim 1 , further comprising a source of oxygen-containing gas ...

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Номер записи: 49
18-05-2017 дата публикации

Vessels and methods for cryopreservation

Номер: US20170135337A1
Автор: Allison Jean Tanner, Ana Marie del Pilar Pardo, Gregory Roger Martin, Jessica Monique Brown, Nicholas Michael Illsley
Принадлежит: Corning Inc

An article including, either: a frame having an interior cavity, and a flexible liner situated within the interior cavity; or a deformable frame having an interior cavity, as defined herein. Either frame can receive a sample, such as liquid having suspended live cells, and cooling freezes the sample to a solid sample. The solid sample can be readily ejected from the article having either a frame with the flexible liner, or the deformable frame, without extensive thawing. The solid sample can be rapidly displaced from the article by the methods and apparatus disclosed herein.

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Номер записи: 50
10-06-2021 дата публикации

System and Method for Paper-Based Cryopreservation

Номер: US20210169070A1
Автор: ALNEMARI Roaa, QASAIMEH Mohammad A.
Принадлежит:

A system and method for cryopreservation of cells or spheroids of cells including obtaining a suspension of the isolated mammalian cells in a medium; applying the suspension to a fibrous substrate comprising a network of interconnected cellulosic fibers defining a plurality of pores therein and allowing the suspension to absorb into the fibrous substrate such that the isolated mammalian cells penetrate and settle to an interior of the fibrous substrate; and cooling the fibrous substrate to or below a temperature of −80° C. 1. A method for cryopreservation of mammalian cells , comprising:obtaining a suspension of isolated mammalian cells in a cryopreservation medium comprising a cryoprotectant;applying the suspension to a fibrous substrate comprising a network of interconnected cellulosic fibers defining a plurality of pores therein by allowing the suspension to absorb into the fibrous substrate such that the isolated mammalian cells penetrate and settle to an interior of the fibrous substrate; andcooling the fibrous substrate to or below a temperature of −80° C.2. The method of claim 1 , wherein the cryoprotectant is selected from a group consisting of glycerol claim 1 , dimethyl sulfoxide (DMSO) claim 1 , and a combination thereof.3. The method of claim 1 , wherein an average diameter of the plurality of pores is from about 50 μm to about 150 μm.4. The method of claim 1 , wherein the cryopreservation medium comprises a hydrogel.5. The method of claim 1 , wherein the cryopreservation medium is substantially free of a hydrogel.6. The method of claim 1 , wherein the fibrous substrate is paper.7. The method of claim 1 , wherein the fibrous substrate further comprises a glycoprotein of an extracellular matrix.8. The method of claim 7 , wherein the glycoprotein is fibronectin.9. The method of claim 1 , wherein the fibrous substrate further comprises a chemically stable resin.10. A system for cryopreservation of mammalian cells claim 1 , comprising:a fibrous substrate ...

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Номер записи: 51
10-06-2021 дата публикации

Cell suspension preparation technique and device

Номер: US20210169636A1
Автор: Fiona M. Wood, Marie L. Stoner
Принадлежит: Avita Medical Pty Ltd

The present invention provides for methods and devices suitable for producing a transplantable cellular suspension of living tissue suitable for grafting to a patient. In applying the method and/or in using the device, donor tissue is harvested, subjected to a cell dissociation treatment, cells suitable for grafting back to a patient are collected and dispersed in a solution that is suitable for immediate dispersion over the recipient graft site.

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Номер записи: 52
28-05-2015 дата публикации

Use of a warmer for promoting a biological reaction

Номер: US20150147808A1
Автор: Gouze Jean-Noël, Vacher Dominique
Принадлежит: LABORATOIRES GENEVRIER

The present invention relates to the use of warmers, or autonomous heat packs, for heating and maintaining a solution on at a suitable temperature, for the period of time required to accomplish a chemical, biochemical or biological reaction, in particular in molecular biology or cell biology applications. Biology kits containing warmers are also part of this invention. 1. A process for carrying out a biological , biochemical or chemical reaction requiring an incubation at a temperature between 30 and 40° C. , the process comprising a step of activating a warmer , the operation of which is based on an exothermic physical or chemical process , and a step of bringing said warmer into contact with a container containing the reagents involved in said reaction.2. The process as claimed in claim 1 , wherein the step of bringing the warmer into contact with the container is carried out by placing the warmer and at least the lower part of the container in a cavity of a support provided for this purpose.3. The process as claimed in claim 1 , wherein the container contains an enzymatic solution and in that the contact between the warmer and the container is maintained for at least 10 minutes.4. The process as claimed in claim 1 , wherein the warmer consists of a hermetic plastic pouch containing a sodium acetate-saturated aqueous solution.5. The process as claimed in claim 4 , wherein the volume of the solution containing the reagents involved in the reaction is between 3 and 10 ml claim 4 , the container containing said solution is a Petri dish having a diameter between 7 and 11 cm or a compartment of said dish and in that the warmer is a disk of which the thickness is between 3 and 7 mm and the diameter is between 7 and 11 cm.6. The process as claimed in claim 2 , wherein the cavity of the support intended to receive the warmer is circular claim 2 , with a diameter between 7 and 11.5 cm claim 2 , and has a depth between 5 and 20 mm.7. The process as claimed in claim 1 , for ...

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Номер записи: 53
26-05-2016 дата публикации

METHOD OF OPERATING CONTINUOUS STERILIZING APPARATUS, CONTINUOUS STERILIZING APPARATUS, FERMENTING SYSTEM, AND CONTINUOUS FERMENTING SYSTEM

Номер: US20160145559A1
Автор: Hayakawa Hiroshi, Mimitsuka Takashi, Morita Ken, OTSUKA Kenichi, Yamamoto Yuki
Принадлежит:

A continuous sterilizing apparatus circulates, through members constituting a flow channel, a sterilizing medium that sterilizes the members to sterilize the members, circulates a sterilizing liquid subjected to sterilization treatment through pipes, performs a series of pieces of treatment including heating treatment, holding treatment, and cooling treatment on the sterilizing liquid, controls a flow rate, a temperature, and a pressure to be preset conditions, and switches a liquid to be circulated through the flow channel from the sterilizing liquid to a liquid to be sterilized. 114-. (canceled)15. A method of operating a continuous sterilizing apparatus that continuously performs a series of pieces of treatment including heating treatment , holding treatment , and cooling treatment on a flow channel through which a liquid to be sterilized circulates to perform sterilization on the liquid to be sterilized , the method comprising:circulating a sterilizing medium that sterilizes members forming the flow channel through the flow channel to sterilize the member;circulating a sterilizing liquid subjected to sterilization treatment through the members, performing the series of pieces of treatment on the sterilizing liquid, and measuring a flow rate, a temperature, and a pressure of the sterilizing liquid subjected to the series of pieces of treatment;comparing the flow rate, the temperature, and the pressure measured with respect to the sterilizing liquid subjected to the series of pieces of treatment with a flow, a temperature, and a pressure as preset conditions and determining whether the measured flow, temperature, and pressure satisfy the conditions; andswitching, when it is determined that the conditions are satisfied at the determining, the liquid to be circulated through the members from the sterilizing liquid to the liquid to be sterilized.16. The method according to claim 15 , wherein the sterilizing medium is steam.17. The method according to claim 15 , ...

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Номер записи: 54
15-09-2022 дата публикации

Jig for cell or tissue cryopreservation

Номер: US20220287297A1
Автор: Atsushi Matsuzawa, Kakeru Yoshida
Принадлежит: Mitsubishi Paper Mills Ltd

The present invention aims to provide a device for cryopreservation providing excellent working efficiency in operations for freezing and thawing cells or tissues. Provided is a device for cryopreservation off a cell or tissue including at least: a body including a strip-shaped tip; and a cap covering the tip of the body, the tip including at least a deposition part comprising a preservation solution absorber, the tip having a length in a minor axis direction of not shorter than 70% of an inner diameter of the cap, the cap including an inner cavity having a length in a major axis direction of not longer than 200% of a length in a major axis direction of the tip.

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Номер записи: 55
15-09-2022 дата публикации

METHOD AND APPARATUS FOR PROCESSING TISSUE SAMPLES

Номер: US20220290092A9
Автор: Bashkin John, CHEAR Kaiwan, Eberhart David, Jovanovich Stevan Bogdan, Leisz Bruce
Принадлежит:

A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues. 1. A system comprising: (i) one or more cartridge interfaces, each configured to engage a cartridge and comprising one or more fluid ports;', (1) one or more sources of liquids and/or gasses;', '(2) one or more fluid lines communicating with the sources and with fluid ports in the cartridge interface; and', '(3) one or more pumps configured to move liquids and/or gasses from the sources into and/or out of the one or more fluid ports;, '(ii) a fluidics subsystem comprising, '(iii) a subsystem comprising an actuator to actuate a tissue disruptor in a cartridge engaged with a cartridge interface (e.g., a mechanical, pneumatic, electromagnetic, or hydraulic actuator); and, '(a) an instrument comprising (i) one or more cartridge ports communicating with the fluid ports in the cartridge interface;', '(ii) a preprocessing chamber comprising an opening and, positioned in the opening, a tissue disruptor configured for mechanical disruption of tissue, wherein the tissue disruptor engages with and is actuated by the actuator when the cartridge is engaged with the cartridge interface; and', '(iii) a processing chamber communicating with the preprocessing chamber, and with one or more cartridge ports and configured to collect a suspension of biological material from the preprocessing chamber., '(b) one or more cartridges, each engaged with one of the cartridge interfaces, wherein each cartridge comprises2. The system of claim 1 , wherein the instrument further comprises:none, one or a plurality of valves configured to direct positive or negative pressure from ...

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Номер записи: 56
15-09-2022 дата публикации

ASEPTIC TISSUE PROCESSING METHOD, KIT AND DEVICE

Номер: US20220290094A1
Автор: Guest Ryan Dominic
Принадлежит:

The present invention concerns a single use aseptic kit comprising: a disaggregation module for receipt and processing of material comprising solid mammalian tissue; and a stabilisation module for storing disaggregated product material, wherein each of said modules comprises one or more flexible containers connected by one or more conduits adapted to enable flow of the tissue material there between; and wherein each of said modules comprises one or more ports to permit aseptic input of media and/or reagents into the one or more flexible containers. The invention further relates to an automated device for semi-automated aseptic disaggregation and/or enrichment and/or stabilisation of cells or cell aggregates from mammalian solid tissue comprising a programmable processor and the single use aseptic kit. The invention further relates to a semi-automatic aseptic tissue processing method. 133-. (canceled)34. A method of aseptic tissue processing , comprising:obtaining a sample that has not been previously cryopreserved comprising cancer cells contained in a closed flexible bag for disaggregation comprising at least one port;aseptically transferring media enzyme solution into the closed flexible bag for disaggregation through a port in the closed flexible bag for disaggregation;subjecting the sample contained in the closed flexible bag for disaggregation to physical and enzymatic disaggregation to disaggregate into single cell or small number aggregate cellular suspensions;aseptically separating the single cell or small number aggregate cellular suspensions and transferring the single cell or small number aggregate cellular suspensions through a port into a closed flexible bag for cell culture;aseptically transferring a culture medium for T-cell expansion into the closed flexible bag for cell culture through a port in the closed flexible bag for cell culture;and,culturing the cells in the closed flexible bag for cell culture.35. The method of claim 34 , wherein the ...

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Номер записи: 57
25-05-2017 дата публикации

SHIELDING DEVICE TO REDUCE THE IMPACT OF ELECTROMAGNETIC RADIATION

Номер: US20170150655A1
Автор: Juhász János, Juhászné Molnár Marianna
Принадлежит:

The present invention relates to a shielding device to reduce the impact of radiation, that contains a container () with a receptacle () and a functional filling () placed into the receptacle () of the container (), where the functional filling () is composed of a collecting component () that contains a mixture of inorganic substances and a utilizing component () that contains a mixture of organic components. 1. A shielding device to reduce the impact of radiation , comprising:a container with a receptacle; anda functional filling positioned in the receptacle of the container, where the functional filling is composed of a collecting component that contains a mixture of inorganic substances and a utilizing component that contains a mixture of organic components,wherein the utilizing component of the functional filling includes a compound with microorganisms that contain lactic acid bacteria in 23% to 27% (v/v) and yeast in 23% to 27% (v/v) dispersed in potassium silicate 16% to 20% (v/v) and/or graphite 8% to 12% (v/v) and/or in a gold and bronze powder 20% to 24% (v/v),wherein the collecting component of the functional filling is a compound composed of cobalt 10% to 14% (v/v) and/or iron 14% to 18% (v/v) and/or molybdenum 10% to 14% (v/v) and/or niobium 13% to 17% (v/v) and/or silicon 12% to 16% (v/v) and/or boron 13% to 17% (v/v) and/or silver 14% to 18% (v/v),wherein the container has a housing in the form of a solid superficies with a filler hole, where the filler hole of the housing is covered by a lid.2. A shielding device as in claim 1 , wherein a sealing at the connection of the housing of the container and its lid is gas-proof.3. A shielding device as in claim 1 , further comprising a porous bearing component impregnated with the utilizing component of the functional filling claim 1 , wherein the porous bearing component permits the utilizing component of the functional filling to be positioned in the receptacle of the container.4. A shielding device as in ...

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Номер записи: 58
11-06-2015 дата публикации

Process and apparatus for the pre-treatment of biomass

Номер: US20150158009A1
Автор: Aart Berthold Kleijn
Принадлежит: HRS Investments Ltd

A process for pretreatment of biomass and an installation for practicing the process, the process including, as well, the subsequent biological treatment and obtaining of biofuel from the biomass. The process is based on the use of at least one scraped surface exchanger and comprises the following steps: heating the biomass to a temperature equal to or lower than 110° C. in an exchanger; further heating the heated biomass so obtained to a temperature between 150 and 175° C. in a scraped surface exchanger; thermal hydrolyzing the biomass at a temperature between 150 and 175° C.; and cooling the thermal hydrolyzed biomass for the subsequent biological treatment thereof.

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Номер записи: 59
24-06-2021 дата публикации

Multi-compartment biological fluid storage device

Номер: US20210187495A1
Автор: Chacon Lourdes Isabel, Sampaio Luiz C., Taylor Doris Anita
Принадлежит:

An storage container for a biological fluid includes, for example, a sequence of elongated compartments interconnected by passageways. The sequence forms a single continuous tube or ribbon having a first end, to which a first port is optionally connected, and a second end, to which a second port is also optionally connected. After filling the container with a biological fluid, multiples closures are formed across the single continuous tube or ribbon, for example across each passageway, to seal the compartments. 1. A storage bag for a biological fluid , comprising:a bottom sheet;a top sheet superimposed on the bottom sheet; wherein a first portion of the top sheet is sealed against a second, corresponding portion of the bottom sheet, wherein the first portion and second portion enclose a ribbon having a first end and a second end, wherein the ribbon is an essentially flat, narrow and long tubular, wherein the ribbon is continuous between the first end and the second end;a first port connected across the first end of the ribbon;a second port connected across the second end of the ribbon;a first male rib attached to one of the top sheet and the bottom sheet along a first cross section of the ribbon;a first female channel attached to the other of the top sheet and the bottom sheet along the first cross-section of the ribbon, wherein the first male rib and the first female channel are configured to form a first closure between the top sheet and the bottom sheet upon mating of the first female channel with the first male rib;a second male rib attached to one of the top sheet and the bottom sheet along a second cross-section of the ribbon, wherein the second cross-section is offset from the first cross-section; anda second female channel attached to the other of the top sheet and the bottom sheet along the second cross-section of the ribbon, wherein the second male rib and the second female channel are configured to form a second closure upon mating of the second female ...

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Номер записи: 60
29-09-2022 дата публикации

Instrument for use in operating cell

Номер: US20220304298A1
Автор: Masashige Kuwayama, Rio KUWAYAMA
Принадлежит: ADVANCED INSTITUTE OF REPRODUCTIVE TECHNOLOGIES

Provided is an instrument having favorable operability when a plurality of cells is operated. An instrument 1 of the present invention includes a plate main body 2 having a well 8 and is used when cells are operated. The well 8 has a bottom 8A and a curved inner peripheral wall 8B that is formed so as to be contiguous to the bottom 8A, and a plurality of protruding portions 13 for partitioning the bottom 8A and the inner peripheral wall 8B into a plurality of portions is provided along the bottom 8A and the inner peripheral wall 8B. Recesses 13A are formed in partitioned portions of the bottom 8A by forming raised portions 13A such that each of the raised portions 13A extends from one of the protruding portions 13 to the bottom 8A, and cells are settled into the recesses 13A.

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Номер записи: 61
29-09-2022 дата публикации

Systems and methods for cell culturing

Номер: US20220306977A1
Автор: Andrew Kozbial, Shashi K. Murthy
Принадлежит: Flaskworks LLC

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

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Номер записи: 62
23-06-2016 дата публикации

Method and system for preparation of substrate for use in anaerobic digestion of organic waste

Номер: US20160177253A1
Автор: Aleksandr Andreevich Smotritckii, Andrey Vladimirovich Smotritskiy, Tatiana Andreevna Smotritskaya
Принадлежит: "bioenergy" LLC

There is disclosed a method for preparation of substrate for use in anaerobic digestion of organic waste. The method can be executed in a substrate pretreatment system, the method comprising: loading of a substrate into the substrate pretreatment system, mixing the substrate with liquid; heating the substrate and liquid to a pre-determined processing temperature; exposing the mixture to radiation; transferring the processed substrate to a bioreactor, during the heating, the method further comprises: degassing the mixture using vacuum extraction; and circulating the mixture past an ultrasonic hydrodynamic radiator.

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Номер записи: 63
22-06-2017 дата публикации

SYSTEM AND METHOD FOR ANAEROBIC DIGESTION OF ANIMAL WASTES

Номер: US20170172055A1
Автор: Shih Jason Chia Hsing
Принадлежит: Holistic Farming, Inc. (HFI)

A new method of disposing of waste for the hog industry is disclosed which avoids use of lagoons. Manure is semi-continuously degritted, anaerobically digested and digested with biomass to produce bio-organic fertilizer and biogas. 1. A method for the semi-continuous treatment of animal waste from an animal containment building comprising:a) periodically removing undiluted animal waste from the animal containment building;b) placing the removed waste in a hydrolytic degritting chamber with sufficient heated water or steam to obtain a mixture of at least about 50 degrees C. to about 60 degrees C. and degritted;c) transfer of at least a portion of the degritted mixture into an anaerobic digester chamber held at a temperature of about 50 degrees C. to about 60 degrees C. and digested; andd) transfer of at least a portion of the digested mixture to a secondary solid phase digester tank, loaded with dry biomass and fermenting the mixture at a temperature of at least about 50 degrees C. and removing the bio-organic fertilizer from the solid-phase digester.2. The method according to wherein the animal waste is from hogs and the containment building is a hog house.3. The method according to wherein the animal waste and heated water are left in the degritter for about 24 hours before removal.4. The method according to wherein the degritted animal waste remains in the anaerobic digester for at least 10 days before a portion is removed.5. The method according to wherein the digestate is transferred by fluid gravity into the secondary solid phase digester loaded with dry biomass and is incubated.6. The method according to wherein the material removed from the secondary digester is further dried before use.7. The method according to wherein the material removed is used as fertilizer.8. The method according to wherein biogas is collected at any of steps c) or d).9. The method according to wherein a plurality of hog houses are connected to a single degritting tank of the present ...

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Номер записи: 64
28-06-2018 дата публикации

SET FOR CULTURE VESSEL TRANSPORT AND CELL/BIOLOGICAL TISSUE TRANSPORT UNIT

Номер: US20180177181A1
Автор: IWAMOTO Yuki, Katou Satoshi, Kobayashi Yoshifumi, KUWABARA Junichi, NAKAJIMA Hidenori
Принадлежит:

A culture container transporting set A includes: a culture container having a vessel made of a bottom wall and a tubular side wall raised from the bottom wall a flexible cover that covers an upper end of the side wall a presser member that is relatively hard and placed on the cover and a holder that holds the culture container the cover and the presser member integral as stacked into an assembled state, by applying pressure from top and bottom. 1. A culture container transporting set comprising:a culture container having at least one vessel including a bottom wall and a tubular side wall raised from the bottom wall;a flexible cover that covers an upper end of the side wall of the vessel;a presser member that is hard and placed on the cover; anda holder that holds together a stacked assembly of the culture container, the cover and the presser member by applying pressure from top and bottom.2. The culture container transporting set according to claim 1 , wherein the cover includes: a thick portion having a relatively large thickness and in intimate contact with the upper end of the side wall; and a thin portion having a relatively small thickness claim 1 , surrounded by the thick portion as viewed in a vertical direction and having gas permeability.3. The culture container transporting set according to claim 1 , wherein the thick portion has a re-sealing ability to seal a syringe needle puncture claim 1 , and in the assembled state claim 1 , the thick portion is in intimate contact with a lower surface of the presser member.4. The culture container transporting set according to claim 1 , wherein the cover has a projected portion accommodated inside the side wall to project toward the bottom wall.5. The culture container transporting set according to claim 1 , wherein the cover is formed of a film having gas permeability.6. The culture container transporting set according to claim 1 , wherein the cover is formed of a film without gas permeability.7. The culture ...

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Номер записи: 65
09-07-2015 дата публикации

Zero-waste method for producing ethanol and apparatus for implementing same

Номер: US20150191751A1
Автор: Ajrat Enikeev, Valerij Galynkin
Принадлежит: Individual

A wasteless method for producing ethanol comprises grain milling, mixing same with water in mashing, heat-treating mixed mass accompanied by two-stage enzymatic hydrolysis, sterilizing hydrolyzed mass, cooling same, performing enzymatic vacuum saccharification of hydrolyzed mass, cooling resulting wort down to fermentation temperature, separating wort into first liquid and first solid phases, additionally separating first solid phase into second solid and second liquid phases, fermenting liquid phases, and distilling ethanol from fermented wort. Second solid phase is dried and used for feed and food production, still bottom from distilling is returned to mashing and also used for irrigation. A plant for implementing the method comprises receiving hopper, grain separator, hammer mill, vibrating screen, mixer, first and second stage mechanic and enzymatic treatment unit for carbohydrate hydrolysis, sterilizer, vacuum saccharifier, at least one pre-thickener including hydrocyclone or decanter centrifuge, additional separator, drying apparatus, and ultrafiltration unit connected to rectification unit distilling ethanol.

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Номер записи: 66
15-07-2021 дата публикации

METHOD AND APPARATUS FOR PROCESSING TISSUE SAMPLES

Номер: US20210214673A1
Автор: Bashkin John, CHEAR Kaiwan, Eberhart David, Jovanovich Stevan Bogdan, Leisz Bruce
Принадлежит:

A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues. 1. A system comprising: (i) one or more cartridge interfaces, each configured to engage a cartridge and comprising one or more fluid ports;', (1) one or more sources of liquids and/or gasses;', '(2) one or more fluid lines communicating with the sources and with fluid ports in the cartridge interface; and', '(3) one or more pumps configured to move liquids and/or gasses from the sources into and/or out of the one or more fluid ports;, '(ii) a fluidics subsystem comprising, '(iii) a subsystem comprising an actuator to actuate a tissue disruptor in a cartridge engaged with a cartridge interface (e.g., a mechanical, pneumatic, electromagnetic, or hydraulic actuator); and, '(a) an instrument comprising (i) one or more cartridge ports communicating with the fluid ports in the cartridge interface;', '(ii) a preprocessing chamber comprising an opening and, positioned in the opening, a tissue disruptor configured for mechanical disruption of tissue, wherein the tissue disruptor engages with and is actuated by the actuator when the cartridge is engaged with the cartridge interface; and', '(iii) a processing chamber communicating with the preprocessing chamber, and with one or more cartridge ports and configured to collect a suspension of biological material from the preprocessing chamber., '(b) one or more cartridges, each engaged with one of the cartridge interfaces, wherein each cartridge comprises2. The system of claim 1 , wherein the instrument further comprises:none, one or a plurality of valves configured to direct positive or negative pressure from ...

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Номер записи: 67
26-07-2018 дата публикации

Applying measurement, control and automation to a dry corn milling ethanol production process to maximize the recovery of ethanol and co-products

Номер: US20180206526A1
Автор: Bret H. MAGNESS, James T. TURNBULL
Принадлежит: Buckman Laboratories International Inc, Cidra Corporated Services LLC

Apparatus features a signal processor or signal processing module configured to: receive signaling containing information about about a measurement of one or more constituents of an output stream from a centrifuge in a dry corn milling process, including to produce Ethanol; and determine corresponding signaling containing information about a real time feedback control of the dry corn milling process, based upon the signaling received. The signal processor or signal processing module is configured to provide the corresponding signaling as control signaling to provide the real time feedback control of the dry corn milling process.

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Номер записи: 68
03-08-2017 дата публикации

ANTIMICROBIAL METHOD FOR FERMENTATION PROCESSES

Номер: US20170218403A1
Автор: Semenza Reed, Welker Michael
Принадлежит:

A system and method for controlling bacteria, especially lactic and acetic acid bacteria in the production of ethanol using an organic oxidizing compound in combination with an inorganic oxidizer is provided. Particularly, a mixture of one or more peroxy acids and one or more peroxide compounds is introduced into a fermentation mash so as to inhibit or reduce levels of bacteria that compete with yeast for the fermentation sugars. The peroxy acid and peroxide compounds largely are consumed during the fermentation process and are generally not present in the fermentation by-products, especially recovered distiller's grains. 1. A method of controlling lactic acid levels within an ethanol fermentation system comprising:introducing peracetic acid and hydrogen peroxide into a fermentation mash located within said ethanol fermentation system, wherein said peracetic acid is introduced into said fermentation mash at a level of less than 50 ppm and said hydrogen peroxide is introduced into said fermentation mash at a level of less than 50 ppm, wherein said fermentation mash comprises an aqueous suspension of fiber, wherein a first portion of said hydrogen peroxide is added to said fermentation mash prior to the introduction of said peracetic acid into said fermentation mash, and wherein said peracetic acid is introduced into said fermentation mash as a mixture with a second portion of said hydrogen peroxide; andfermenting said fermentation mash with an ethanol-producing yeast, at least a portion of said peracetic acid and said hydrogen peroxide being present in said fermentation mash during said fermenting step at levels sufficient to prevent lactic acid levels within said system from exceeding 0.20% by weight of a liquid portion of said fermentation mash.2. The method according to claim 1 , wherein said peracetic acid is introduced into said fermentation mash at a level of between 5 to 30 ppm.3. The method according to claim 1 , wherein said hydrogen peroxide is introduced ...

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Номер записи: 69
11-07-2019 дата публикации

PROCESSING BIOMASS

Номер: US20190211501A1
Автор: Medoff Marshall
Принадлежит:

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. 1. A method comprising:converting a treated biomass feedstock, utilizing an enzyme or a microorganism, to produce a product, wherein the feedstock has been treated by:preparing an untreated biomass feedstock by reducing one or more dimensions of individual pieces of biomass;irradiating the untreated biomass feedstock; andmechanically treating the irradiated biomass feedstock to reduce one or more dimensions of individual pieces of the pretreated biomass feedstock.2. The method of claim 1 , wherein the treated biomass feedstock has an uncompressed bulk density of less than about 0.6 g/cm3 claim 1 , wherein bulk density is determined using ASTM D1895B.3. The method of claim 1 , wherein the treated biomass feedstock includes fibers having a length/diameter (L/D) ratio of at least about 5.4. The method of claim 1 , wherein mechanically treating the irradiated biomass feedstock comprises milling the feedstock.5. The method of claim 1 , wherein the untreated biomass feedstock comprises a cellulosic or lignocellulosic material.6. The method of claim 5 , wherein the biomass feedstock is selected from the group consisting of paper claim 5 , paper products claim 5 , paper waste claim 5 , wood claim 5 , particle board claim 5 , sawdust claim 5 , agricultural waste claim 5 , sewage claim 5 , silage claim 5 , grasses claim 5 , rice hulls claim 5 , bagasse claim 5 , cotton claim 5 , jute claim 5 , hemp claim 5 , flax claim 5 , bamboo claim 5 , sisal claim 5 , abaca claim 5 , straw claim 5 , corn cobs claim 5 , corn stover claim 5 , switchgrass claim 5 , alfalfa claim 5 , hay claim 5 , coconut hair claim 5 , seaweed claim 5 , algae claim 5 , and mixtures thereof.7. The method ...

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Номер записи: 70
12-08-2021 дата публикации

DEVICE AND METHOD FOR STORING LIVE MICROALGAE

Номер: US20210246404A1
Автор: Bashan Oded, Bashan Ohad, Berzin Isaac, COUTURE Sam C.
Принадлежит: Vaxa Technologies Ltd.

A device for storing live algae is disclosed. The device may include: a closed container, at least partially transparent to light, the container is configured to hold live algae aquaculture at a predetermined temperature; at least one light source for providing light to the closed container; a COsource for providing COto the closed container; an air circulation system for circulating air inside the closed container; and a controller for controlling the at least one light source to illuminate an internal space of the closed container in an amount sufficient to keep the algae aquaculture alive but inhibits reproduction of the algae for at least 4 weeks. 1. A device for storing live algae , comprising:a closed container, at least partially transparent to light, the container is configured to hold live algae aquaculture at a predetermined temperature;at least one light source for providing light to the closed container;{'sub': 2', '2, 'a COsource for providing COto the closed container;'}an air circulation system for circulating air inside the closed container; anda controller for controlling the at least one light source to provide light to the closed container in an amount sufficient to keep the algae aquaculture alive but inhibits reproduction of the algae for at least 4 weeks.2. The device of claim 1 , wherein the controller is further configured to control the COsource to provide COin an amount sufficient to keep the algae aquaculture alive but inhibits reproduction of the algae.3. The device of claim 1 , wherein the intensity and duration of light are determined based on at least one of: the type of the live algae claim 1 , the size of the closed container and a required live algae density.4. The device of claim 2 , wherein the amount of COis determined based on at least one of: the type of the live algae claim 2 , the size of the closed container and a required live algae density.5. The device of claim 1 , wherein the controller is configured to adjust the ...

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Номер записи: 71
11-08-2016 дата публикации

METHOD AND PLANT FOR PRODUCING BIOGAS FROM LIGNOCELLULOSE-CONTAINING BIOMASS

Номер: US20160230134A1
Автор: FICHTER Enrico, Horn Jens, Kühling Jan, LÜDTKE Oliver, Pollert Georg, SCHLIMBACH Michael
Принадлежит:

The invention relates to a method for producing biogas from lignocellulose-containing biomass, preferably from straw, and to a plant for carrying out said method. 1. A method for producing biogas from lignocellulose-containing biomass , preferably from straw , which comprises the following steps:a) Mixing the lignocellulose-containing biomass with an average particle size smaller than 200 mm, preferably smaller than 20 mm, with aqueous solutions at a temperature of 60-100° C., especially 70-85° C., especially with process water and/or with the aid of steam;b) Heating the lignocellulose-containing biomass at 130-200° C., preferably 150-180° C., and residence of the thus heated lignocellulose-containing biomass for a duration of 5-120 mins, preferably 10-30 mins, advantageously in a continuous flow reactor space for thermal digestion;c) Anaerobic fermentation of the heat-treated lignocellulose-containing biomass by means of microorganisms and recovery of the biogas formed.2. A method according to claim 1 , characterized in that after step a) and before step b) claim 1 , as an additional process step a further step a1) is performed claim 1 , in which an anaerobic fermentation of the mixed lignocellulose-containing biomass by means of microorganisms and recovery of the biogas formed takes place.3. A method according to claim 1 , characterized in that before implementation of step b) claim 1 , as an additional process step a step a2) is performed claim 1 , wherein a heat exchange operating on the countercurrent principle takes place between the lignocellulose-containing biomass obtained in step a) or the lignocellulose-containing biomass subjected to a first anaerobic fermentation obtained in step a1) claim 1 , especially after prior adjustment of the dry substance content to 7-20 wt. % claim 1 , and the heat-treated lignocellulose-containing biomass obtained in step b).4. A method according to claim 3 , characterized in that through the heat exchange operating in ...

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Номер записи: 72
10-08-2017 дата публикации

Organic Waste Digester System

Номер: US20170226466A1
Автор: Grillo Paul, Secovich Bruce
Принадлежит:

An organic waste digester system is provided. The system includes a heated hopper unit within the housing to receive organic waste. An agitation mechanism mixes the organic waste along with a microbe mixture to aid breakdown of the waste. Liquefied organic waste is discharged through an outlet and conveyed to a drying unit downstream of the hopper unit. The drying unit includes a microwave dryer unit. 1. An organic waste digester system , comprising:a hopper unit, the hopper unit including a bin to receive organic waste, an agitation mechanism to mix the organic waste, and an outlet through which liquefied organic waste is discharged;a drying unit downstream of the hopper unit to receive and dry the liquefied organic waste from the hopper unit.2. The system of claim 1 , wherein the drying unit comprises a microwave drying unit comprising a microwave generation chamber and a microwave application chamber.3. The system of claim 2 , wherein the microwave generation chamber includes a plurality of microwave units claim 2 , each microwave unit including a waveguide disposed to direct microwave radiation into the microwave application chamber.4. The system of claim 2 , further comprising one or more cooling fans in the microwave generation chamber to cool the microwave units.5. The system of claim 2 , further comprising one or more openings between the microwave generation chamber and the microwave application chamber to direct heated air into the microwave application chamber.6. The system of claim 5 , wherein the one or more openings are disposed through an associated waveguide.7. The system of claim 2 , further comprising a microwave suppression chamber disposed at an outlet of the microwave application chamber.8. The system of claim 7 , further comprising a conveyor disposed to travel through the microwave application chamber claim 7 , the conveyor configured to receive the liquefied organic waste from the hopper unit.9. The system of claim 2 , further comprising a ...

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Номер записи: 73
09-07-2020 дата публикации

INTEGRATED WASTE CONVERSION SYSTEM AND METHOD

Номер: US20200216760A1
Автор: INGOLFSSON Oddur, INGOLFSSON Sigurdur, MATTHIASSON Asgeir
Принадлежит:

An entirely water-based, energy self-sufficient, integrated in-line waste management system is provided for comprehensive conversion of all organic fractions of municipal and wider community waste to fuels suitable for use in transportation, with all solid residues converted to high nutrition compost. The system is based on a combination of pre-treatment, involving alkaline hydrolysis and saponification; three-way separation of the pre-treated waste into different streams that are each directed to suitable further processing including fuel production; which includes biodiesel generation in a continuous-flow catalytic esterification unit, and anaerobic digestion to produce methane or other small molecule biofuel. Remaining solids are converted to compost in a quasi-continuous process. 1. A waste conversion system , comprisingat least one pre-treatment unit for receiving a stream of waste of which at least a portion thereof being organic waste, the pretreatment unit comprising at least one continuous-flow steam explosion reactor;at least one separation unit, for receiving a stream of pre-treated waste from said pre-treatment unit, the separation unit comprising at least one conditioning section and at least one separation section for the separation of conditioned waste into at least one fat/oil component and at least one aqueous component;at least one biodiesel production unit, for generating biodiesel from the at least one fat/oil component;at least one digestion unit, for anaerobic digestion and/or fermentation of the at least one aqueous component; andat least one composting unit, for the generation of compost from solid organic material.2. The system according to claim 1 , wherein said steam explosion reactor comprises a high-pressure retention section and a pressure relief section and wherein said high-pressure retention section comprises at least one adjustable-speed conveyor for transporting said stream of waste through said section.3. The system according to ...

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Номер записи: 74
25-07-2019 дата публикации

HIGH DENSITY CELL BANKING METHODS

Номер: US20190223433A1
Автор: Buser Claudia, DONG Haodi, Jin Xiaoxia
Принадлежит:

The current disclosure provides a method for the creation of a high-density cryopreserved cell bank using perfusion culture techniques and non-centrifugal concentration of cells. Methods of production using this high-density cryopreserved cell bank are also provided. 1. A non-centrifugal method for producing a high-density frozen mammalian cell bank , the method comprising:a) culturing mammalian cells in a perfusion bioreactor to a first cell density by continuously removing growth medium from a culture and replacing with fresh growth medium, wherein said bioreactor is coupled to a cell retention system comprising an alternating tangential flow filtration system including a filter;{'sup': '8', 'b) non-centrifugally concentrating the cells cultured in the perfusion bioreactor coupled to the cell retention system, wherein the cells are concentrated to a second cell density greater than the first cell density by removing the growth medium from the culture using the filter and, thereby reducing the volume of the growth medium to produce a concentrated cell population of about 1×10cells/mL;'}c) cryopreserving the concentrated cell population to produce a high-density frozen mammalian cell bank, wherein the high-density frozen mammalian cell bank has a post-thaw viability of at least 90%, wherein the pH and DO of the culture are controlled by non-automated methods and, wherein the bioreactor is rocked at between 5 rpm and 22 rpm with a rock angle of between 5° and 10°.2. (canceled)3. The method of claim 1 , wherein the filter has a surface area of at least 0.3 m.4. The method of claim 1 , wherein the filter has a surface area of about 0.5 to about 1.0 m claim 1 , about 0.7 to about 0.8 m claim 1 , about 2.0 to about 3.0 m claim 1 , or 4 to about 5 m claim 1 , optionally wherein the filter has a pore size of about 0.2 μm.510-. (canceled)11. The method of claim 1 , wherein the cryopreserving comprises adding DMSO to the concentrated cell population at a final concentration ...

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Номер записи: 75
16-07-2020 дата публикации

SALT MANAGEMENT SYSTEM FOR PORTABLE RENEWABLE ENERGY MICROGENERATION SYSTEM

Номер: US20200223726A1
Автор: SASSOW Nicolas W.
Принадлежит:

A renewable energy microgeneration apparatus is disclosed that includes a mixing tank that mixes waste with a liquid, a buffer tank that receives and pre-warms the mixed waste, a pasteurization tank that pasteurizes on the pre-warmed mixed waste, a digestion tank that performs anaerobic digestion on the pasteurized waste, a de-watering device that separates liquid digestate and removes salt from the liquid, sensors that measure salinity and biogas quality, and a controller. The controller causes the transfer of digestate from the digestion tank to the pasteurization tank to the dewatering device, causes the de-watering device to separate the liquid and remove the salt from the liquid, monitors the salinity of the liquid and the quality of biogas using the sensors, and causes the mixing of the liquid with the waste and adjusts the feed rate of the waste to reduce the salinity of the waste and increase methane production. 1. A portable and modular renewable energy microgeneration apparatus comprising:a mixing tank that is configured to mix incoming waste with a liquid;a buffer tank that is configured to receive the waste from the mixing tank and pre-warm the waste in preparation pasteurization;a pasteurization tank that is configured to perform pasteurization on the waste received from the buffer tank;a digestion tank that is configured to perform anaerobic digestion on waste received from the pasteurization tank;a de-watering device that is configured to separate liquid digestate and to remove salt from the liquid separated from the digestate;first sensors disposed in the buffer tank and the digestion tank that are configured to measure salinity;a second sensor disposed in the digestion tank that is configured to measure biogas quality; and cause the transfer of digestate from the digestion tank to the pasteurization tank after anaerobic digestion of the waste occurs,', 'cause the transfer of digestate from the pasteurization tank to the dewatering device after the ...

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Номер записи: 76
23-08-2018 дата публикации

SYSTEM AND METHOD FOR ANAEROBIC DIGESTION OF ANIMAL WASTES

Номер: US20180235139A1
Автор: Shih Jason Chia Hsing
Принадлежит: Holistic Farming, Inc. (HFI)

A new method of disposing of waste for the hog industry is disclosed which avoids use of lagoons. Manure is semi-continuously degritted, anaerobically digested and digested with biomass to produce bio-organic fertilizer and biogas. 1. A method for the semi-continuous treatment of animal waste from an animal containment building comprising:a) periodically removing undiluted animal waste from the animal containment building;b) placing the removed waste in a hydrolytic degritting chamber with sufficient heated water or steam to obtain a mixture of at least about 50 degrees C. to about 60 degrees C. and degritted;c) transfer of at least a portion of the degritted mixture into an anaerobic digester chamber held at a temperature of about 50 degrees C. to about 60 degrees C. and digested; andd) transfer of at least a portion of the digested mixture to a secondary solid phase digester tank, loaded with dry biomass and fermenting the mixture at a temperature of at least about 50 degrees C. and removing the bio-organic fertilizer from the solid-phase digester.2. The method according to wherein the animal waste is from hogs and the containment building is a hog house.3. The method according to wherein the animal waste and heated water are left in the degritter for about 24 hours before removal.4. The method according to wherein the degritted animal waste remains in the anaerobic digester for at least 10 days before a portion is removed.5. The method according to wherein the digestate is transferred by fluid gravity into the secondary solid phase digester loaded with dry biomass and is incubated.6. The method according to wherein the material removed from the secondary digester is further dried before use.7. The method according to wherein the material removed is used as fertilizer.8. The method according to wherein biogas is collected at any of steps c) or d).9. The method according to wherein a plurality of hog houses are connected to a single degritting tank of the present ...

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Номер записи: 77
31-08-2017 дата публикации

PORTABLE RENEWABLE ENERGY MICROGENERATION SYSTEM

Номер: US20170247278A1
Автор: SASSOW Nicolas W.
Принадлежит:

A portable renewable energy microgeneration system is disclosed. The system comprises one or more holding tanks that are configured to perform anaerobic digestion on waste in a multi-phase process using bacteria and a controller configured to automatically control the multi-phase process and to re-use the bacteria. The controller re-uses the bacteria by removing at least a portion of the liquid from the waste after anaerobic digestion is performed on the waste and using the at least a portion of the liquid to wet other waste and repeat the multi-phase process. 1. A portable renewable energy microgeneration apparatus comprising:one or more holding tanks that are configured to perform anaerobic digestion on waste in a multi-phase process; anda controller comprising a processor configured to execute computer code to control the apparatus to automatically perform the multi-phase process in the one or more holding tanks and to re-use the bacteria, 'a first phase in which the waste is wetted with liquid comprising bacteria, is heated to a first temperature, and remains at the first temperature for a period time, and', 'wherein the multi-phase process comprisesa second phase performed after the first phase in which anaerobic digestion is performed with the bacteria at a second temperature that is different than the first temperature, and removing at least a portion of the liquid from the waste after anaerobic digestion is performed on the waste, and', 'using the at least a portion of the liquid to wet other waste and repeat the multi-phase process., 'wherein re-using the bacteria comprises2. The apparatus of claim 1 , wherein the multi-phase process further comprises:macerating the waste into smaller pieces, andmoving the macerated waste to at least one of the one or more holding tanks.36-. (canceled)7. The apparatus of claim 1 , further comprising a de-watering unit that is configured to generate solid fertilizer by drying what remains of the waste after anaerobic ...

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Номер записи: 78
20-11-2014 дата публикации

Device for vitrification and/or reanimation of oocytes, embryos or blastocysts

Номер: US20140342454A1
Автор: Carl Swindle, Fred Burbank, Michael Jones
Принадлежит: Mariposa Biotechnology Inc

Disclosed herein are devices, methods, systems and kits adapted for vitrification and/or reanimation of oocytes, embryos or blastocysts. The device includes a straw and a filter, wherein the straw comprises a lumen traversing through the straw and has a proximal section, a middle section and a distal section and wherein the filter is affixed in the straw and comprises a plurality of pores having a diameter smaller than the diameter of said oocytes, embryos or blastocysts but large enough to allow the passage of a fluid composition therethrough.

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Номер записи: 79
07-09-2017 дата публикации

Large horizontal device for continuous methane fermentation and method thereof

Номер: US20170253842A1
Автор: Yang Hong
Принадлежит:

A large-type horizontal device and a method for continuous methane fermentation belong to dry biogas fermentation technology. The whole distribution of a fermentation compartment uses a U-shape plane layout which is a snap-back type and uses a material propeller. The material propeller has two axes and two blades and is constantly occluded with counter rotation. The irreversible propulsion of materials can be realized through counter rotation of two occluded blades. The propeller is set at the bottom of the main partition of the fermentation compartment. Since the impeller blades adopt a long side design in rotation axis directions, a large amount materials can be propelled. The propel ability of propeller can be changed through changing of rotation speed. Counter rotation of two occluded blades can realize material propeller without material reverting. The inlet and outlet entrances of the reactor in the disclosure are near to the ground and can be operated conveniently to, therefore, save energy. The homogeneous output of materials and entire plug-flow can be realized at the same time without material mixing in the whole process. This makes fermentation more complete. 1. (canceled)2. (canceled)3. A large horizontal device for continuous methane fermentation , the device comprises:a hydrolysis feed compartment, a hydrolysis heating feeder, a hydrolysis feed heater, a hydrolysis feeder, a hydrolysis feed inoculation and homogenizing compartment, a hydrolysis inoculator, a hydrolysis fermentation compartment, a hydrolysis feed distributor, a hydrolysis materials propeller, a hydrolysis material ejection balancer, a hydrolysis ejector, a hydrolysis gas stirring distributor, a hydrolysis gas circulation draught fan; a methane fermentation feeder, a methane fermentation and inoculation homogenizing compartment, a methane fermentation material inoculator, a methane fermentation compartment, a methane fermentation feed distributor, a methane fermentation material ...

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Номер записи: 80
15-08-2019 дата публикации

APPARATUS AND METHOD FOR PROCESSING SAMPLES CONTAINING BIOLOGICAL CELLS

Номер: US20190249133A1
Автор: Bornmann Gerd, Eberhardt Jens, Opel Matthias, Unglaub David
Принадлежит:

An apparatus for the automated processing of samples containing biological cells—in particular, of blood samples or other cell samples is provided. The apparatus has at least the following components: a sample receiving device configured to receive one or more samples; an auxiliary material receiving device configured to receive one or more auxiliary materials; a sample carrier receiving device configured to receive one or more sample carriers; a discharge device configured to discharge one or more samples from a discharge position; and a capture device configured to capture one or more discharged samples in a capture position to obtain one or more prepared samples; wherein the distance between the discharge position and the capture position can be adjusted to a prespecified height, and the apparatus further includes at least one temperature control device for controlling the temperature of at least one of the sample carriers. 1. An apparatus for the automated processing of a sample containing biological cells , the apparatus comprising:a sample receiving device configured to receive the sample;an auxiliary material receiving device configured to receive auxiliary materials;a sample carrier receiving device configured to receive sample carriers;a discharge device configured to discharge the sample from a discharge position;at least one temperature control device configured to control a temperature of at least one of the sample carriers, anda capture device configured to capture the sample discharged from the discharge position in a capture position to obtain a prepared sample, and a distance between the discharge position and the capture position being adjusted to a prespecified height.2. The apparatus according to claim 1 , wherein the sample containing the biological cells is a blood sample or another cell sample.3. The apparatus according to claim 1 , further comprising a housing in which components of the apparatus are arranged claim 1 , wherein in the housing ...

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Номер записи: 81
14-09-2017 дата публикации

SPRAY FREEZING

Номер: US20170259185A1
Автор: BISGAARD-FRANTZEN Hans, Jensen Allan, MADSEN Michelle
Принадлежит: CHR. HANSEN A/S

The present invention relates to an improved method for preservation of e.g. microorganisms, especially lactic acid bacteria, said method includes spray freezing. 1. A process for preserving microorganisms (esp. LAB (Lactic Acid Bacteria)) , such as preserving by freezing and drying , comprising the following steps:a) preparing droplets of an aqueous (or liquid) suspension containing the microorganisms, e.g. by spraying the suspension; andb) contacting the droplets with a drying gas; andc) contacting the droplets resulting from the preceding step with a cryogenic gas; andd) optionally subjecting the resulting frozen product from the preceding step to a further drying step, such as drying under reduced pressure, e.g. freeze-drying; ande) optionally packaging the microorganisms, such as in an air-tight and/or moisture-tight package.2. A process for drying a microorganism (esp. a LAB) containing suspension , characterized in that:c) an aqueous suspension containing microorganisms is sprayed into a cryogenic gas in a spray chamber; andd) the frozen product from the preceding step is collected and freeze dried until a water activity of less than 0.20 is achieved.3. A process for drying a microorganism (esp. a LAB) containing suspension , characterized in that:b) an aqueous suspension containing microorganisms is sprayed into a drying gas in a spray chamber;c) the product from the preceding step is contacted with a cryogenic gas in a chamber; andd) the frozen powder from the preceding step is collected and freeze dried until a water activity of less than 0.20 is achieved.4. A process for drying (or removing liquid from) a solution or suspension containing proteins (such as enzymes , hormones , human proteins , therapeutically active proteins or lipoproteins) or microorganisms (such as bacteria , LAB , yeasts , fungi , plant cells , animal cells , or vira) , characterized by:a) Preparing droplets of the suspension or solution, e.g. by spraying the solution or suspension; ...

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Номер записи: 82
14-09-2017 дата публикации

PRIMARY MAMMALIAN CELL CULTURE SYSTEMS AND METHODS

Номер: US20170260490A1
Автор: NOVIK Erik, PAREKH Amit, PLUDWINSKI Eric, SHRIRAO Anil
Принадлежит: HUREL CORPORATION

Primary mammalian cell culture systems, methods of making primary mammalian cell culture systems, and methods of culturing primary mammalian cells are provided. In some embodiments the systems and methods utilize an exothermic chemical process as a heat source for culturing and shipping cultures comprising primary mammalian cells. 1. A cell culture system comprising:a first container and a second container, the first container disposed within the second container;a cell culture plate disposed within the first container; anda heat source disposed inside of the second container and outside of the first container.2. (canceled)3. The cell culture system of claim 1 , wherein the first and second containers are dimensioned to provide a space between an inner wall of the second container and an outer wall of the first container.4. (canceled)5. The cell culture system of claim 3 , wherein the heat source is positioned between the inner wall of the second container and the outer wall of the first container.6. The cell culture system of claim 1 , wherein the cell culture plate contains a culture of adherent primary mammalian cells.7. The cell culture system of claim 6 , wherein the adherent primary mammalian cells are hepatocytes.8. The cell culture system of claim 6 , wherein the cell culture plate comprises a base claim 6 , a cover claim 6 , and a layer of elastomeric material disposed between the base and cover to provide a liquid barrier to retain the culture media in the well.910-. (canceled)11. The cell culture system of claim 1 , wherein the heat source is an exothermic chemical process.1216-. (canceled)17. A method of making a cell culture system claim 1 , comprising:A) providing a first container, a second container, a cell culture plate, and a heat source;B) establishing a primary mammalian cell culture in the cell culture plate; and 1) the first container is disposed within the second container;', '2) the cell culture plate comprising the primary mammalian cell ...

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Номер записи: 83
22-09-2016 дата публикации

Rapid Pretreatment

Номер: US20160273009A1
Автор: Lumpkin Robert E.
Принадлежит:

Disclosed herein are methods, systems, and compositions for the pretreatment of biomass within seconds with low inhibitor formation. The pretreatment process is used to convert biomass to a fuel or other useful chemicals by subjecting the feedstock to a rapid retention time under pressure and temperature and/or chemical reactant. The system includes a continuously-operating valve discharge apparatus to discharge pretreated feedstock while maintaining uniform pressure on the pretreatment system. 1. An industrial scale method for pretreating at least one dry ton of biomass per day , the method comprising:a. feeding the biomass at a rate of at least one dry metric ton (MT) of biomass per day into an extrusion system comprising a barrel defining an inner chamber comprising a feeder zone and a reaction zone; andb. treating the biomass at an elevated temperature and pressure within the reaction zone for less than about 20 seconds to produce a pretreated biomass composition comprising a liquid fraction comprising monosaccharides and solid particles comprising cellulose.2. The method of claim 1 , wherein the extrusion system further comprises one or more rotatable screws configured to move the biomass through the extrusion system from the feeder zone and through the reaction zone.3. The method of claim 2 , wherein the one or more rotatable screws comprise one or more sections that are configured to form one or more plugs from the biomass to separate the inner chamber into two or more zones claim 2 , including the feeder zone and the reaction zone.4. (canceled)5. The method of claim 3 , comprising two rotatable screws.6. (canceled)7. The method of claim 1 , further comprising adding a liquid to the biomass prior to the reaction zone.8. (canceled)9. (canceled)10. (canceled)11. (canceled)12. The method of claim 1 , wherein the biomass is treated for about 5 to 15 seconds in the reaction zone.13. (canceled)14. The method of claim 1 , wherein the elevated temperature is about ...

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Номер записи: 84
11-12-2014 дата публикации

Recovery of higher alcohols from dilute aqueous solutions

Номер: US20140360376A1
Автор: Aharon M. Eyal, Andrew C. Hawkins, Aristos A. Aristidou, David A. Glassner, Fudu Miao, Kent Evans, Patrick R. Gruber, William A. Evanko
Принадлежит: Gevo Inc

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

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Номер записи: 85
20-09-2018 дата публикации

A method and an apparatus for increasing concentration of soluble carbohydrate containing fraction, a soluble carbohydrate containing fraction, a solid fraction and their use

Номер: US20180265938A1
Автор: Juha Tamper, Mauno Miettinen, Sami Turunen
Принадлежит: UPM Kymmene Oy

The invention relates to a method and an apparatus for producing a soluble carbohydrate containing fraction (10), in which lignocellulose material (3) formed by treating plant based raw material (1) is conducted into a separation stage (4). The method comprises at least one solid-liquid separation stage (4) for separating a soluble carbohydrate containing fraction (10) and/or a washing filtrate (12) from lignocellulose material (3), and at least a part of the soluble carbohydrate containing fraction (10) and/or the washing filtrate (12) is recirculated to the lignocellulose material (3) for increasing concentration of the soluble carbohydrate containing fraction, and solids (11) and at least a part of the soluble carbohydrate containing fraction (10) are supplied out from the separation stage. Further, the invention relates to the soluble carbohydrate containing fraction and the solid fraction, and their uses.

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Номер записи: 86
28-09-2017 дата публикации

MODULAR SINGLE-USE KITS AND METHODS FOR PREPARATION OF BIOLOGICAL MATERIAL

Номер: US20170275582A1
Автор: BENDIS GREGORY G., Cafferata Robert L., FRILLING LESLIE E., GRANFIELD GARY A., MURRAY John D.
Принадлежит:

Disclosed herein are standardized and optimized, modular single-use kits and methods for sterile or non-sterile preparation of biological material, such as cells or tissue, for immediate use, further preparation, or storage. These single-use kits and methods facilitate and improve a healthcare practitioner's ability to prepare biological material for research, diagnostic and therapeutic purposes in a convenient, consistent, repeatable, safe and effective manner. 187-. (canceled)88. A standardized and optimized , modular , single-use kit for sterile or non-sterile preparation of biological material , the single-use kit comprising:at least one modular single-use packaging frame;at least one modular single-use tray disposed within the packaging frame comprised in the single-use kit, wherein at least one single-use tray disposed within the packaging frame comprised in the single-use kit has an identifying label;at least one single-use component organized within any single-use tray disposed within the packaging frame comprised in the single-use kit; andprinted instructions for use comprising a sequence of steps directing use of the single-use kit for the sterile or non-sterile preparation of biological material, wherein one or more of the steps of the instructions corresponds to (correlates with) the identifying label on one or more of any single-use tray disposed within the packaging frame comprised in the single-use kit,wherein a plurality of the labeled trays are disposed within the packaging frame comprised in the kit, with each labeled tray having at least one component organized therein,wherein the plurality of labeled trays are labeled sequentially, with numbers or letters in ascending or descending order, and one or more steps of the instructions correspond to (correlate with) the sequentially labeled trays, andwherein the kit has a single packaging frame (referred to herein as a preparation sub-kit) that is disposed with at least one tray organized with syringes ...

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Номер записи: 87
28-09-2017 дата публикации

System and method for processing biomass

Номер: US20170275583A1
Автор: Petteri Salonen
Принадлежит: Individual

System ( 100 ) and method for processing biomass. The system comprises a combined heat and power plant ( 102 ), an interface ( 114 ) for feeding biogas to a traffic fuel production unit, interfaces ( 114 ) to a district heating system ( 106 a ) and an electrical grid ( 106 b ), and a hydrolysis device ( 108 ), a digestion device ( 110 ), a dryer ( 116 ) and a heat recovery unit ( 112 ), which are operatively coupled for transferring heat, intermediate products and final products of the process, wherein raw biomass is received into the Fuel hydrolysis device ( 108 ), biomass processed by the hydrolysis device ( 108 ) is fed to the digestion device ( 110 ), biogas obtained in the digestion device ( 110 ) is fed to the traffic fuel production unit ( 104 ), heat is recovered from the hydrolysis device ( 108 ), biomass processed by the digestion device ( 110 ) is dried by the heat recovered from the hydrolysis device ( 108 ), heat is recovered from the dryer ( 116 ), heat recovered from the dryer ( 116 ) is fed to the hydrolysis device ( 108 ) to be used in pre-heating of the received raw biomass, heat recovered from the dryer ( 116 ) is fed to the district heating ( 106 a ), and production of electricity is fueled by the dried biomass from the dryer ( 116 ).

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Номер записи: 88
29-08-2019 дата публикации

DETACHABLE DISSOLVED OXYGEN SENSOR INTERFACE FOR SINGLE-USE BIOREACTOR/MIXER

Номер: US20190264163A1
Автор: Adkins Michalle J.A., Ahmed Taufiq, Bowlds Ryan L., Dierker Andrew S., HU Jinbo, Mason Marc R., Ruch Tyrel L., Simon John W., Sumrall Rick J.
Принадлежит:

An interface for coupling a dissolved oxygen sensor to a single-use bioreactor container is provided. A dissolved oxygen (DO) window membrane is operably coupled to the single-use container and configured to position a DO sensor at least partially within the single-use container. In some embodiments, a DO window body mounts the DO window membrane at a distal end thereof. The DO window body can include a slide lock for facilitating positioning of a DO sensor within the DO window body. Additionally, the DO window body may include at least one heat exchange fin. 1. An interface for coupling a dissolved oxygen sensor to a single-use container , the interface comprising:a dissolved oxygen (DO) window membrane operably coupled to the single-use container and configured to position a DO sensor at least partially within the single-use container.2. The DO sensor interface of claim 1 , and further comprising a DO window body configured to couple to a port of the single-use container claim 1 , the DO window body mounting the DO window membrane at an end that is configured to pass through the port of the single-use container and be disposed within the single-use container.3. The DO sensor interface of claim 2 , and further comprising a sensor adaptor sealed to the DO window body and being configured to receive the DO sensor.4. The DO sensor interface of claim 1 , and further comprising a DO sensor disposed within the DO window body claim 1 , the DO sensor having a DO sensor membrane positioned adjacent the DO window membrane claim 1 , the DO sensor having a temperature sensitive element disposed claim 1 , relative to the port of the single-use container claim 1 , within the single-use container.5. The DO sensor interface of claim 1 , and further comprising an end cap sealingly coupling the DO window membrane to the DO window body.6. The DO sensor interface of claim 5 , wherein the end cap is configured to couple to the DO window body via a snap operation.7. The DO sensor ...

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Номер записи: 89
27-09-2018 дата публикации

MODULAR SINGLE-USE KITS AND METHODS FOR PREPARATION OF BIOLOGICAL MATERIAL

Номер: US20180273893A1
Автор: BENDIS GREGORY G., Cafferata Robert L., FRILLING LESLIE E., GRANFIELD GARY A., MURRAY John D.
Принадлежит:

Disclosed herein are standardized and optimized, modular single-use kits and methods for sterile or non-sterile preparation of biological material, such as cells or tissue, for immediate use, further preparation, or storage. These single-use kits comprise at least one single-use component necessary for the preparation of biological material in accordance with a particular method as set forth in the instructions for use included with each particular type of single-use kit that set forth a unique method for using that particular type of single-use kit. The single-use kit of the invention comprises at least one modular, single-use packaging frame wherein at least one modular, single-use tray with an identifying is disposed, wherein at least one single-use component is organized. The identifying label on each modular single-use tray corresponds to (correlates with) a sequence of steps set forth in the instructions included with each single-use kit. The single-use kits of the invention facilitate and improve a healthcare practitioner's ability to prepare biological material for research, diagnostic and therapeutic purposes in a convenient, consistent, repeatable, safe and effective manner. 1. A standardized and optimized , modular , single-use kit for sterile or non-sterile preparation of biological material , the single-use kit comprising:at least one modular single-use packaging frame;at least one modular single-use tray disposed within the packaging frame comprised in the single-use kit, wherein at least one single-use tray disposed within the packaging frame comprised in the single-use kit has an identifying label;at least one single-use component organized within any single-use tray disposed within the packaging frame comprised in the single-use kit; andprinted instructions for use comprising a sequence of steps directing use of the single-use kit for the sterile or non-sterile preparation of biological material, wherein one or more of the steps of the instructions ...

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Номер записи: 90
15-10-2015 дата публикации

APPARATUS AND METHOD FOR SIMULTANEOUS OPERATION OF REFINING CELLULOSIC BIOMASS AND MIXING ENZYMES

Номер: US20150291928A1
Автор: KIM Taewan, KOO Bonwook, KOO Minsu
Принадлежит:

An apparatus comprises: a hopper for feeding biomass; a horizontal extruder comprising a cylindrical housing and a rotatable screw mounted in the housing, in which one side of the housing is connected to the hopper, and an outlet provided at the other side of the housing is connected to an inlet for feeding biomass into a refiner; and a refiner comprising a stator disc having a cooling water space formed on the outer peripheral surface thereof, one or more enzyme feeding holes provided in a refining region adjacent to the circumference of the stator disc, and a biomass inlet provided at the central portion thereof, and a refining disc which is disposed so as to form a gap with the inner surface of the stator disc and to have a refining region opposite to that of the stator disc and is rotated by a separate motor. 1. An enzyme mixing-biomass refining apparatus comprising:(a) an extruder comprising a cylindrical housing and a rotatable screw mounted in the housing, wherein one side of the housing is connected to a feedstock feeding unit, and an outlet provided at the other side of the housing is connected to a biomass inlet of a refiner; and(b) the refiner comprising: a stator disc having a cooling water unit formed on the outer peripheral surface thereof, one or more enzyme feeding holes provided in a refining region thereof, and a biomass inlet provided at the central portion thereof; and a refining disc which is provided so as to form a gap with the inner surface of the stator disc and to have a refining region opposite to that of the stator disc and is rotated by a separate motor.2. The enzyme mixing-biomass refining apparatus of claim 1 , wherein the holes are penetratingly formed in a refining region adjacent to the circumference of the stator disc claim 1 , and are connected to an enzyme inlet pipe connected to an enzyme feeding unit.3. The enzyme mixing-biomass refining apparatus of claim 1 , wherein the holes are separately connected to a unit for feeding one ...

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Номер записи: 91
18-10-2018 дата публикации

Use of a Warmer For Promoting a Biological Reaction

Номер: US20180298334A1
Автор: Gouze Jean-Noël, Vacher Dominique
Принадлежит: LABORATOIRES GENEVRIER

The present invention relates to the use of warmers, or autonomous heat packs, for heating and maintaining a solution at a suitable temperature, for the period of time required to accomplish a chemical, biochemical or biological reaction, in particular in molecular biology or cell biology applications. Biology kits containing warmers are also part of this invention. 115-. (canceled)16. A process for carrying out an in vitro biochemical reaction for preparing a cell suspension suitable for application to the skin of a patient , the process comprising:i) activating a warmer, the operation of which is based on an exothermic physical or chemical process,ii) bringing said warmer into contact with a container containing a solution comprising trypsin by placing the warmer and at least the lower part of the container in a cavity of a support,iii) placing a skin tissue sample comprising cells suitable for grafting to the skin of the patient in the solution comprising trypsin and incubating at from 30° C. to 40° C. for at least five minutes, andiv) isolating cells disassociated from the skin sample and suspending the cells in a solution, to thereby provide a cell suspension suitable for application to the skin of a patient.17. The process as claimed in claim 16 , wherein the contact between the warmer and the container is maintained for at least 10 minutes.18. The process as claimed in claim 16 , wherein the volume of the solution comprising trypsin is from 3 and to 10 ml claim 16 , the container containing said solution comprising trypsin is a Petri dish having a diameter of from 7 to 11 cm or a compartment of said dish and wherein the warmer is a disk of which the thickness is from 3 to 7 mm and the diameter is from 7 to 11 cm.19. The process as claimed in claim 16 , wherein the cavity of the support is circular claim 16 , with a diameter from 7 to 11.5 cm claim 16 , and a depth from 5 to 20 mm.20. The process as claimed in claim 16 , wherein the solution containing trypsin ...

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Номер записи: 92
18-10-2018 дата публикации

NUCLEIC ACID DETECTION AND QUANTIFICATION METHOD AND COMPOSITIONS

Номер: US20180298428A1
Автор: EDWARD Jessica, KING Michael R, LANGE Amy, SON Sona, SPENCER Joel D, Veldkamp Ardean
Принадлежит:

This invention relates to a method of detecting a gene. The invention also relates to a method of determining the Amplification expression level of a gene. The invention also relates to compositions for use in these methods. 1. A method of quantifying the expression level of a gene from a microorganism , the method comprising the steps of:recovering the nucleic acid from a sample stabilized on a card,amplifying the nucleic acid; andquantifying the expression level of the gene, wherein a forward primer, and a reverse primer are used for the amplification.2. The method of further comprising the step of hybridizing a probe to the nucleic acid to specifically identify the gene.3. The method of wherein the reverse primer comprises a sequence selected from the group consisting of SEQ ID NO: 6 and SEQ ID NO: 8.4. The method of wherein the nucleic acid is DNA.5. The method of wherein the nucleic acid is RNA.6. The method of wherein the nucleic acid is amplified using PCR.7. The method of wherein the PCR is reverse transcription PCR.8. The method of wherein the PCR is reverse transcription-quantitative PCR.9. The method of wherein the probe is fluorescently labeled.10. The method of wherein the primer is fluorescently labeled.11Vibrio harveyi, Vibrio campbellii, Vibrio fluvialisVibrio parahaemolyticus.. The method of wherein the microorganism is selected from the group consisting of claim 1 , and12Clostridium perfringens, Campylobacter jejuniCampylobacter coli.. The method of wherein the microorganism is selected from the group consisting of claim 1 , and13. The method of wherein the sample is a sample from an animal.14. The method of wherein the sample is an aquatic sample.15. The method of wherein the aquatic sample is from a fish hatchery.16. The method of wherein the aquatic sample is from a shrimp pond.17. The method of wherein the sample is an agricultural sample.18. The method of wherein the agricultural sample is from animal litter.19. The method of wherein the ...

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Номер записи: 93
27-10-2016 дата публикации

PROCESS COMPRISING SULFUR DIOXIDE AND/OR SULFUROUS ACID PRETREATMENT AND ENZYMATIC HYDROLYSIS

Номер: US20160312249A1
Автор: Foody Brian, Foody Patrick J., MacDonald Daniel, Tolan Jeffrey S.
Принадлежит:

The present invention provides a process for producing a fermentation product from a lignocellulosic feedstock. The process comprises soaking a lignocellulosic feedstock in an aqueous solution to produce a soaked feedstock. The soaked feedstock is at least partially dewatered and the at least partially dewatered feedstock is subjected to pretreating in the presence of sulfur dioxide, sulfurous acid or a combination thereof to produce a pretreated feedstock composition. The pretreated feedstock composition is fed to an enzymatic hydrolysis in which the concentration of dissolved solids fed to the enzymatic hydrolysis is at least 50% (w/w) of the concentration of dissolved solids in the pretreated feedstock composition. The cellulose in the pretreated feedstock composition is hydrolyzed with cellulase enzymes in the presence of the dissolved solids to produce glucose. The glucose is fermented to produce the fermentation product. 1. A process for producing a fermentation product from a lignocellulosic feedstock comprising:(i) soaking the cellulosic feedstock in a liquid comprising water to produce a soaked feedstock;(ii) at least partially dewatering the soaked feedstock to produce an at least partially dewatered feedstock;(iii) feeding the at least partially dewatered feedstock to a heating device;(iv) adding steam and acid to the heating device, said steam heating the at least partially dewatered feedstock to a first temperature to provide a heated feedstock, said acid comprising at least one of sulfur dioxide and sulfurous acid;(v) pretreating the heated feedstock in the presence of the acid in a pretreatment reactor to produce a pretreated feedstock, said pretreatment reactor disposed downstream of said heating device;(vi) hydrolyzing cellulose in the pretreated feedstock with cellulase enzymes to produce glucose, wherein the concentration of dissolved solids in the pretreated feedstock fed to the hydrolysis is at least about 50% of the concentration of dissolved ...

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Номер записи: 94
12-11-2015 дата публикации

Biomass processing device and processing method

Номер: US20150322398A1
Автор: Hideaki Mizuno, Tomoki Hayakawa
Принадлежит: Tsukishima Kikai Co Ltd

A biomass treatment device and a biomass treatment method, in which contamination is prevented and a final product can be highly concentrated. The biomass treatment device includes a pretreatment device for pretreating a biomass, a slurrying device for slurrying the pretreated biomass, and a saccharification device for saccharifying the slurried biomass with a saccharifying enzyme. A return line for returning a part of an internal fluid in the saccharification device to the slurrying device is provided, and the pretreated biomass from the pretreatment device and the internal fluid returned through the return line are mixed in the slurrying device so as to generate biomass slurry.

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Номер записи: 95
10-10-2019 дата публикации

CELL SUSPENSION PREPARATION TECHNIQUE AND DEVICE

Номер: US20190307550A1
Автор: STONER Marie L., WOOD Fiona M.
Принадлежит:

The present invention provides for methods and devices suitable for producing a transplantable cellular suspension of living tissue suitable for grafting to a patient. In applying the method and/or in using the device, donor tissue is harvested, subjected to a cell dissociation treatment, cells suitable for grafting back to a patient are collected and dispersed in a solution that is suitable for immediate dispersion over the recipient graft site. 115-. (canceled)16. A method for treating a skin condition of a patient , the method comprising:peri-operatively preparing a cell suspension from a skin tissue sample from the patient, wherein the cell suspension comprises cells from the skin tissue sample suspended in a nutrient solution; andadministering the prepared cell suspension to a recipient region on the patient without in vitro culturing.17. The method of claim 16 , wherein the cell suspension comprises dermal cells and epidermal cells claim 16 , including dermal cells from a dermal surface facing a dermal-epidermal junction of the skin tissue sample and epidermal cells facing the dermal-epidermal junction.18. The method of claim 17 , wherein preparing the cell suspension comprises subjecting the skin tissue sample to an enzyme to dissociate the dermis layer and the epidermis layer at the dermal-epidermal junction.19. The method of claim 18 , wherein the skin tissue sample is subjected to the enzyme for between 5 minutes and 60 minutes.20. The method of claim 19 , wherein the skin tissue sample is subjected to the enzyme for between 10 minutes and 30 minutes.21. The method of claim 18 , wherein the enzyme comprises trypsin or trypsin-EDTA.22. The method of claim 18 , wherein the enzyme is selected from the group consisting of dispase claim 18 , collagenase claim 18 , thermolysin claim 18 , pronase claim 18 , hyaluronidase claim 18 , pancreatin claim 18 , elastase claim 18 , and papain.23. The method of claim 17 , wherein preparing the cell suspension comprises ...

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Номер записи: 96
17-10-2019 дата публикации

STEM CELL MANUFACTURING SYSTEM, STEM CELL INFORMATION MANAGEMENT SYSTEM, CELL TRANSPORT APPARATUS, AND STEM CELL FROZEN STORAGE APPARATUS

Номер: US20190313633A1
Автор: INABA Kiyonori, ODA Masaru, TANABE KOJI
Принадлежит:

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state. 132-. (canceled)33. A stem cell information management system for performing an integrated management of information in an entry process of receiving a manufacturing request for manufacturing stem cells from somatic cells , a transport process of transporting somatic cells collected from a somatic cell donor or stem cells produced from the somatic cells , an examination process of examining the somatic cells or the stem cells , a manufacturing process of manufacturing the stem cells from the somatic cells , and a stock process of stocking the stem cells , the system comprising:a memory unit configured to store a collection schedule for collecting the somatic cells from the somatic cell donor, an examination schedule for examining the somatic cells, a production schedule for one or more production device(s) configured to produce the stem cells from the somatic cells, a cryopreservation schedule for one or more cryopreservation device(s) configured to cryopreserve the produced stem cells, and a stock schedule for a stock site at which the ...

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Номер записи: 97
17-10-2019 дата публикации

STEM CELL MANUFACTURING SYSTEM, STEM CELL INFORMATION MANAGEMENT SYSTEM, CELL TRANSPORT APPARATUS, AND STEM CELL FROZEN STORAGE APPARATUS

Номер: US20190313634A1
Автор: INABA Kiyonori, ODA Masaru, TANABE KOJI
Принадлежит:

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state. 155-. (canceled)56. A cell transport apparatus for transporting somatic cells collected from a somatic cell donor(s) to one or more closed production device(s) configured to produce stem cells from somatic cells , or for transporting the stem cells to a stem cell stock site , the apparatus comprising:a somatic cell collection vial for containing the collected somatic cells and equipped with a first individual identification device containing donor identification information for identifying the somatic cell donor and production device identification information for identifying the production device, or a stem cell freezing vial for containing stem cells produced in the production device and subsequently frozen and equipped with a second individual identification device containing the donor identification information, the production device identification information, and stock site identification information for identifying a stock site for stocking the stem cells;a transport container configured to contain one or more of the somatic cell ...

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Номер записи: 98
24-10-2019 дата публикации

STEM CELL MANUFACTURING SYSTEM, STEM CELL INFORMATION MANAGEMENT SYSTEM, CELL TRANSPORT APPARATUS, AND STEM CELL FROZEN STORAGE APPARATUS

Номер: US20190320648A1
Автор: INABA Kiyonori, ODA Masaru, TANABE KOJI
Принадлежит:

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state. 168-. (canceled)69. A stem cell frozen storage apparatus for cryopreserving stem cells produced from somatic cells collected from a somatic cell donor , the apparatus comprising:a stem cell freezing vial(s) released from one or more closed production device(s) and containing frozen stem cells;one or more cryopreservation device(s) configured to cryopreserve the stem cell freezing vial(s);a storehouse that stores the cryopreservation device(s); anda first conveyer device configured to convey the cryopreservation device(s) into and out of the storehouse, a container unit configured to contain one or more of the stem cell freezing vials, and', 'a refrigerant chamber configured to contain a refrigerant for freezing the stem cell freezing vial(s)., 'the cryopreservation device(s) comprising70. The stem cell frozen storage apparatus according to claim 69 , the cryopreservation device(s) comprising at least one of:a remaining amount sensor configured to detect a remaining amount of the refrigerant;a temperature sensor configured to measure ...

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Номер записи: 99
01-12-2016 дата публикации

SYSTEM AND METHOD OF PRESERVATION, STORAGE AND TRANSPORT OF BIOLOGICAL MATERIALS

Номер: US20160348060A1
Автор: GERALDES FERNANDES Vitor Manuel, JOAQUIM RODRIGUES Miguel Angelo
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The present application discloses a system and method for preservation, storage and transport of biological materials by the use of at least one deformable container configured with at least one inlet at one end, an outer surface greater than 80% of the total surface of the cavities () of the support () and a width and length sufficient for it to be placed in contact with all the heat transfer plates () of the support (). The support () has at least two cavities () delimited by the heat transfer plates (), with means for temperature control, side walls () and at least one main front door (). 1. A system of preservation , storage and transport of biological materials which comprises the following elements:a support with at least two cavities delimited by heat transfer plates with means for temperature control, side walls and at least one main front door;at least one deformable container having at least one inlet at one end, one outer surface greater than 80% of the total surface of the cavities of the support and sufficient length and width for it to be placed in contact with all of the heat transfer plates of said support.2. A system of preservation claim 1 , storage and transport of biological materials according to us claim 1 , wherein the deformable container is a deformable container of single bag or a deformable container of multiple bags.3. A system of preservation claim 1 , storage and transport of biological materials according to claim 1 , wherein the deformable container supports a volume greater than 80% of the total volume of the cavities of the support.4. A system of preservation claim 1 , storage and transport of biological materials according to claim 1 , wherein the deformable container supports a volume equal to or greater than the total volume of the cavities of the support when they are empty.5. A system of preservation claim 1 , storage and transport of biological materials according to claim 1 , wherein the deformable container of multiple bags ...

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Номер записи: 100