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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 8447. Отображено 100.

05-01-2012 дата публикации

Sampling devices and methods for concentrating microorganisms

Номер: US20120003626A1

Автор: James E. Aysta, Kurt J. Halverson, Manjiri T. Kshirsagar, Neil Percy

Принадлежит: Individual

The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.

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Номер записи: 1

26-01-2012 дата публикации

Hydrodynamic extraction of oils from photosynthetic cultures

Номер: US20120021497A1

Автор: Mario C. Larach

Принадлежит: KAIBIOENERGY

The presently described invention relates to the method of extraction of oils from photosynthetic cultures using hydrodynamic cavitation technology for the production of biofuels or other products. This method is referred to herein as Hydrodynamic extraction.

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Номер записи: 2

01-03-2012 дата публикации

Cell isolation apparatus

Номер: US20120052559A1

Автор: Akane Suzuki, Sunao Takeda, Takahiro SHIOYAMA, Tatsuya Shimizu, Teruo Okano, Yuji Haraguchi

Принадлежит: Nihon Kohden Corp, TOKYO WOMENS MEDICAL UNIVERSITY

A cell isolation apparatus includes: a chamber body which includes a chamber into which tissue is to be introduced; an isolation member which is moved in the chamber and which is to collide with the tissue to isolate a cell; and a controlling portion which controls an operation of the isolation member in the chamber body.

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Номер записи: 3

24-05-2012 дата публикации

Device and process for isolating and cultivating live cells on a filter or extracting their genetic material

Номер: US20120129164A1

Автор: Yvon Cayre

Принадлежит: SCREENCELL

The process for isolating live cells on a filter or extracting their genetic material. The process comprises the steps of attaching, at least temporarily, a filter to a lower opening of a compartment having, in addition, an air inlet; inserting into the compartment a liquid carrying the cells; and attaching, in an impermeable manner, a needle, at least temporarily, to the compartment opening, the filter being positioned between the needle and the interior volume of the compartment. The process further comprises the steps of perforation, with the needle, of a plug of a vacuum tube with negative pressure relative to ambient pressure; and aspiration, by means of negative pressure from the vacuum tube, of the liquid through the filter, the filter retaining the cells.

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Номер записи: 4

31-05-2012 дата публикации

Systems and methods for magnetic separation of biological materials

Номер: US20120132593A1

Автор: Aaron Joseph Dulgar-Tulloch, Arvind Kumar Tiwari, James William Bray, Munish Vishwas Inamdar, Shankar Chandrasekaran, Sunil Srinivasa Murthy

Принадлежит: General Electric Co

A magnetic separator comprising a separation chamber is provided. The separation chamber comprises a having an inlet and at least one outlet opposite the inlet in a downstream direction, and a magnetic source operatively coupled to the separation chamber. The magnetic source comprises a plurality of magnets that can be selectively turned off and on to create a dynamic magnetic field in the separation chamber.

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Номер записи: 5

02-08-2012 дата публикации

Method and apparatus for continuous flow membrane-less algae dewatering

Номер: US20120193297A1

Автор: Armin R. Völkel, David K. Fork, Jeonggi Seo, John S. Fitch, Meng H. Lean

Принадлежит: Palo Alto Research Center Inc

In one aspect of the presently described embodiments, the system comprises an inlet to receive at least a portion of the fluid containing algae, a curved channel within which the fluid containing algae flows in a manner such that the neutrally buoyant algae flow in a band offset from a center of the curved channel, a first outlet for the fluid with algae within which the band flows, and, a second outlet for the remaining fluid.

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Номер записи: 6

02-08-2012 дата публикации

Devices and method for enrichment and alteration of cells and other particles

Номер: US20120196273A1

Автор: Bruce L. Carvalho, Lotien Richard Huang, Mehmet Toner, Paul Vernucci, Ravi Kapur, Thomas A. Barber, Zihua Wang

Принадлежит: Individual

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

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Номер записи: 7

16-08-2012 дата публикации

Apparatus and process for production of an encapsulated cell product

Номер: US20120208255A1

Автор: Christine A. Singer, John H. Evans, Iv, Lisa Beckler Andersen

Принадлежит: Geosynfuels LLC

A process for production of an encapsulated cell product, the process comprises the steps of concentrating cells from a propagation medium using a tangential flow filtration system. Mixing the concentrated cells with an encapsulation medium to form a cell encapsulation mixture. Polymerizing, gelling, or cross-linking the cell encapsulation mixture to form an encapsulated cell product.

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Номер записи: 8

20-09-2012 дата публикации

Megakaryocyte and Platelet Production from Stem Cells

Номер: US20120238020A1

Автор: Mauro P. Avanzi, W. Beau Mitchell

Принадлежит: New York Blood Center Inc

Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

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Номер записи: 9

01-11-2012 дата публикации

Methods for isolating stem cells

Номер: US20120276629A1

Автор: HEE YOUNG LEE

Принадлежит: Individual

Disclosed is a method for isolating stem cells. The method uses a specially designed apparatus including: a container containing an aspirate; a piston having an outer diameter corresponding to the inner diameter of the container and having at least one through-hole; and a connection tube adapted to feed an enzyme or a washing solution into the container through the through-hole, having a tip connected to the through-hole, and connected to an external tube or another container containing the enzyme or washing solution at the other end thereof. The method includes pulling the piston backward to form a negative pressure in the container containing the aspirate and to allow the enzyme or washing solution to enter the container containing the aspirate through the connection tube and the through-hole of the piston.

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Номер записи: 10

20-12-2012 дата публикации

Apparatus And Method For Cell Exploitation

Номер: US20120322047A1

Автор: Bassil Akra

Принадлежит: Ludwig Maximilians Universitaet Muenchen LMU

The present invention relates to an apparatus ( 1 ) for automated exploitation of cells from a sample, comprising a plurality of dispensing containers ( 2 ) for the separated storage of fluid media, each dispensing container comprising an outlet ( 21 ) wherein a media delivery arrangement ( 3 ) is associated to each of the dispensing containers ( 2 ) and a first valve ( 22 ) is connected to each of the outlets ( 21 ), a plurality of collecting containers ( 4 ) for the collection of fluid media, each collecting container ( 4 ) comprising an inlet ( 41 ) wherein a second valve ( 42 ) is connected to the inlet ( 41 ) of each collecting container, and a mounting device ( 5 ) for mounting the sample, the mounting device comprising an inlet ( 51 ) being connected to the first valve ( 22 ), and an outlet ( 52 ), wherein the outlet ( 52 ) of the mounting device is connected to the second valve ( 42 ). Further, the present invention enables a method for automated cell exploitation from a sample by the use of the apparatus ( 1 ).

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Номер записи: 11

27-12-2012 дата публикации

Combined acoustic micro filtration and phononic crystal membrane particle separation

Номер: US20120325747A1

Автор: Bart Lipkens, Edward A. Rietman, Jason Dionne

Принадлежит: Flodesign Sonics Inc

A system is provided that includes one or more acoustic microfilters through which is flowed a mixture of a fluid and a particulate to selectively filter particles from the fluid. Also included are one or more phononic crystal units coupled to the acoustic microfilter(s) to further selectively filter particles from the fluid. Related apparatus, systems, techniques and articles are also described.

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Номер записи: 12

03-01-2013 дата публикации

Apparatus and method for processing biological material

Номер: US20130005032A1

Автор: James C. Laird, Kyungyoon Min, Thomas E. Dudar

Принадлежит: Baxter International Inc

The application discloses an apparatus and method for processing biological material, including a suspension of cells.

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Номер записи: 13

07-03-2013 дата публикации

Method and system for the production of cells and cell products and applications thereof

Номер: US20130058907A1

Автор: Beverly Norris, Darrell P. Page, Grant Adams, Karl Patrick Bongers, Mark Hirschel, Martin Peder Crep, Michael J. Gramer, Robert J. Wojciechowski, Scott Waniger, Thiem Chan Duong Wong

Принадлежит: Biovest International Inc

A cell culture system for the production of cells and cell derived products includes a reusable instrumentation base device incorporating hardware to support cell culture growth. A disposable cultureware module including a cell growth chamber is removably attachable to the instrumentation base device. The base device includes microprocessor control and a pump for circulating cell culture medium through the cell growth chamber. The cultureware module is removably attached to the instrumentation base device. Cells are introduced into the cell growth chamber and a source of medium is fluidly attached to the cultureware module. Operating parameters are programmed into the microprocessor control. The pump is operated to circulate the medium through the cell growth chamber to grow cells or cell products therein. The grown cells or cell products are harvested from the cell growth chamber and the cultureware module is then disposed.

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Номер записи: 14

28-03-2013 дата публикации

DEVICE FOR VIRAL INACTIVATION OF LIQUID MEDIA

Номер: US20130078702A1

Автор: Dhanasekharan Muthukumar

Принадлежит: Genzyme Corporation

An apparatus () capable of viral inactivation of liquid media includes at least one coaxial cylinder () constructed of an outer cylinder () and an inner cylinder (), a liquid media inlet (), at least one emitter of type C ultraviolet radiation (), and a liquid media outlet (). The inner cylinder has an outer diameter adapted to form a gap () between the outer diameter of the inner cylinder and the inner diameter of the outer cylinder. The media flows in a substantially cyclonic flow path along the gap. The at least one emitter of type C ultraviolet radiation is placed inside the inner cylinder. The outlet is connected to the outer cylinder at, or proximal to, an end of the outer cylinder opposite the inlet. 1. An apparatus capable of viral inactivation of cell culture media comprising: i) an outer cylinder having a length, an inner diameter, and an outer diameter;', 'ii) an inner cylinder coaxial with the outer cylinder, having a length substantially equal to the length of the outer cylinder and having an outer diameter adapted to form a gap between the outer diameter of the inner cylinder and the inner diameter of the outer cylinder through which the cell culture media flows in a substantially cyclonic flow path along the gap;, 'a) at least one coaxial cylinder comprisingb) a cell culture media inlet connected to the outer cylinder proximal to an end of the outer cylinder and configured to flow the cell culture media along the substantially cyclonic flow path along the gap;c) at least one emitter of type C ultraviolet radiation placed inside the inner cylinder so as to emit the type C ultraviolet radiation towards the cell culture media to be treated with the type C ultraviolet radiation and thereby inactivate viruses in the cell culture media; andd) a cell culture media outlet connected to the outer cylinder proximal to an end of the outer cylinder opposite the inlet.2. The apparatus of claim 1 , wherein the cell culture media flows along the gap at a flow rate in ...

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Номер записи: 15

11-04-2013 дата публикации

Single Use Centrifuge System for Highly Concentrated and/or Turbid Feeds

Номер: US20130089917A1

Автор: Kessler Stephen B., Marro T. David

Принадлежит: PNEUMATIC SCALE CORPORATION

A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates. 1. A centrifuge system for centrifugal separation of a high turbidity feed in a large cell culture batch , having a single use component comprising: a rotatable upper flange, wherein the upper flange includes a central opening,', 'a rotatable lower flange, and', 'a rotatable hollow cylindrical core having an axis, an interior, and an exterior, and which is attached to and extends between the upper flange and the lower flange, wherein the interior of the core is in fluid connection with the exterior of the core,, 'a core structure which is at least semi-rigid and which includes'}a rotatable flexible liner which is sealed, with respect to fluids, to the upper flange and the lower flange,a separation chamber, axially aligned with and surrounding the core and bounded by the flexible liner, having an outer wall which at least partly contains the liner and an inner wall which at least partly contains the exterior of the core, and having an upper end adjacent the upper flange and a lower end adjacent the lower flange, a central feed tube which has an open ...

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Номер записи: 16

18-04-2013 дата публикации

Apparatus and method for using ultrasonic radiation for controlled fragmentation of chains of nucleic acids

Номер: US20130092524A1

Автор: Babur Hadimioglu, Kapil Dev, Smriti Sharma, Vibhu Vivek

Принадлежит: Microsonic Systems Inc

Example methods and systems are directed to controlled fragmentation of genetic samples that include chains of nucleic acid. Waveform inputs to a transducer configured as Fresnel Annular Sector Actuator (FASA) are used to focus acoustic energy at the genetic sample in a controlled fragmentation process that reduces the genetic sample to a desired average fragment size for the resulting chains of nucleic acid.

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Номер записи: 17

18-04-2013 дата публикации

CELL SEPARATING APPARATUS AND CELL SEPARATING METHOD

Номер: US20130095564A1

Автор: YOSHIOKA Satomi

Принадлежит: SEIKO EPSON CORPORATION

A cancer cell separating apparatus includes: a flow channel including an antibody fixation area having antibodies which bind specifically to cancer cells fixed thereon, wherein the cancer cells and non-cancer cells are separated using a difference in velocity of movement between the cancer cells and the non-cancer cells in cell slurry introduced into the flow channel. 1. A cancer cell separating apparatus comprising:a flow channel including an antibody fixation area having antibodies which bind specifically to cancer cells fixed thereon,wherein the cancer cells and non-cancer cells are separated using a difference in velocity of movement between the cancer cells and the non-cancer cells in cell slurry introduced into the flow channel.2. The cancer cell separating apparatus according to claim 1 , wherein the antibody fixation area is provided on an entire surface of the flow channel.3. The cancer cell separating apparatus according to claim 1 , wherein the flow channel includes:a first portion; anda second portion and a third portion bifurcated from an one end portion of the first portion,wherein an antibody fixation prohibited area having no antibody fixed thereon is provided on the surface of the first portion on the side of the second portion, andthe antibody fixation area is provided on the surface of the first portion on the side of the third portion.4. The cancer cell separating apparatus according to claim 1 , further comprising:a first electrode pair and a second electrode pair, whereintime lengths required for the cancer cells and the non-cancer cells to pass from the first electrode pair to the second electrode pair are detected.5. The cancer cell separating apparatus according to claim 4 , further comprising a dielectric migration electrode pair.6. The cancer cell separating apparatus according to claim 5 , wherein the dielectric migration electrode pair is controlled on the basis of the time length.7. A cancer cell separating method comprising:introducing ...

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Номер записи: 18

25-04-2013 дата публикации

OIL-BASED MATERIAL-PRODUCING METHOD AND OIL-BASED MATERIAL-PRODUCING APPARATUS

Номер: US20130102047A1

Автор: ISHIZUKA Akinori, Tsukahara Yasunori, YOSHINO Iwao

Принадлежит:

An oil-based material-producing method includes a microwave irradiation step of irradiating oil-based material-producing microorganisms with microwaves. The oil-based material-producing method may also include a collecting step of collecting an oil-based material produced by the oil-based material-producing microorganisms after the microwave irradiation step. 1. An oil-based material-producing method , comprising a microwave irradiation step of irradiating oil-based material-producing microorganisms with microwaves.2. The oil-based material-producing method according to claim 1 , wherein claim 1 , in the microwave irradiation step claim 1 , microwaves are irradiated in a presence of a microwave responsive material claim 1 , which is either one of a microwave-absorbing material and a microwave-sensitive material.3. The oil-based material-producing method according to claim 2 , wherein the microwave responsive material is able to flow.4. The oil-based material-producing method according to claim 3 , wherein the microwave responsive material has a shape for collecting electric field of microwaves.5. The oil-based material-producing method according to claim 2 , wherein the microwave responsive material is immobilized.6. The oil-based material-producing method according to claim 2 , wherein the microwave responsive material is at least any one of a dielectric claim 2 , a conductive substance claim 2 , and a magnetic substance.7. The oil-based material-producing method according to claim 1 , further comprising a collecting step of collecting an oil-based material produced by the oil-based material-producing microorganisms after the microwave irradiation step.8. The oil-based material-producing method according to claim 1 , wherein the oil-based material-producing microorganisms are oil-based material-producing microalgae.9. The oil-based material-producing method according to claim 1 , wherein claim 1 , in the microwave irradiation step claim 1 , microwaves having two or ...

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Номер записи: 19

02-05-2013 дата публикации

Continuous flow micro-crusher

Номер: US20130104362A1

Автор: Joanna Aizenberg, Joseph Ashley Taylor, Paul Kolodner, Thomas N. Krupenkin

Принадлежит: Lucent Technologies Inc

The present invention provides an apparatus comprising a substrate and first and second disks. The disks are rotatably located over the substrate, each disk having an outer circumference with teeth thereon. The first disk is positioned to interleave one or more of its teeth with the teeth of the second disk. The substrate includes a channel with an exit port located near the teeth of one of the disks. Another apparatus comprises at least one disk rotatably located over a substrate and in a well of the substrate, the disk having an outer circumference with teeth thereon. The disk is positioned to provide a maximum distance of less than about 10 microns between each one the teeth and a nearest wall defining the well. The substrate includes a channel with an exit port located near the teeth of the disk.

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Номер записи: 20

02-05-2013 дата публикации

Method and apparatus for growing photosynthetic organisms

Номер: US20130109008A1

Автор: Arnold Mangott, Howard Kneebone, Kirsten Heimann, Marc Rene Stammbach, Rocky De Nys

Принадлежит: MBD Energy Ltd

A biological cultivation system for the culture of photosynthetic organisms including at least one cultivation chamber permitting exposure of the culture medium to natural and/or artificial light and including; a light transmissive wall or walls defining a gas space; and a culture medium containment area below the gas space; one or more fluid inlets positioned within the culture medium containment area; and one or more gas outlets in communication with the gas space; a control unit operatively connected to a gas flow control device, the gas flow control device controlling the flow of gas in through the fluid inlets and out through the fluid outlets to control the conditions within the cultivation chamber.

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Номер записи: 21

02-05-2013 дата публикации

Methods of and devices for capturing circulating tumor cells

Номер: US20130109011A1

Автор: Daniel Sang-won PARK, Dimitris E. Nikitopoulos, Michael C. Murphy, Sudheer D. Rani, Taehyun Park

Принадлежит: Louisiana State University and Agricultural and Mechanical College

A device and methods are provided for efficient and quick capture of target cells through a main microchannel having capture elements immobilized thereon and manipulating a velocity profile of a sample as it passes through the main microchannel. The cell capture device may have a main microchannel with a depth slightly larger than the diameter of the target cells and a plurality of side microchannels. The side microchannels may have a depth smaller than the diameter of the target cells. The device and methods may be used for early cancer detection.

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Номер записи: 22

06-06-2013 дата публикации

SHEET-SHAPED CELL CULTURE DISSOCIATION SYSTEM AND METHOD

Номер: US20130143315A1

Автор: Yamamoto Takashi

Принадлежит:

Disclosed is a system for dissociating a sheet-shaped cell culture into individual cells. The system, so configured as to minimize the amount of damage to cells when dissociating a sheet-shaped cell culture into individual cells, in one form includes: (i) a reaction unit that dissociates the sheet-shaped cell culture; (ii) a sensor unit that acquires information relating to a particle size distribution of cells inside the reaction unit; and (iii) an analysis unit that computes the particle size distribution of the cells from the information acquired by the sensor unit and determines and outputs a dissociation state. 1. A system for dissociating a sheet-shaped cell culture into individual cells , comprising:(i) a reaction unit which dissociates the sheet-shaped cell culture;(ii) a sensor unit which acquires information about a particle size distribution of cells existing in the reaction unit; and(iii) an analysis unit which calculates the particle size distribution of cells from the information acquired by the sensor unit, thereby determining and outputting a state of dissociation.2. The system as defined in claim 1 , wherein the sheet-shaped cell culture contains a skeletal myoblast.3. The system as defined in claim 1 , further comprising a reaction control unit which controls the dissociation of cells in the reaction unit.4. The system as defined in claim 2 , further comprising a reaction control unit which controls the dissociation of cells in the reaction unit.5. The system as defined in claim 3 , wherein the reaction control unit includes at least one of an environment control unit claim 3 , a chemical action control unit claim 3 , and a mechanical action control unit.6. The system as defined in claim 4 , wherein the reaction control unit includes at least one of an environment control unit claim 4 , a chemical action control unit claim 4 , and a mechanical action control unit7. A system for determining a state of dissociation of a sheet-shaped cell culture in a ...

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Номер записи: 23

13-06-2013 дата публикации

Use of oxyhydrogen microorganisms for non-photosynthetic carbon capture and conversion of inorganic and/or c1 carbon sources into useful organic compounds

Номер: US20130149755A1

Автор: John S. Reed, Lisa Dyson

Принадлежит: Kiverdi Inc

Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including bio-fuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO 2 in one or more steps of the process.

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Номер записи: 24

04-07-2013 дата публикации

Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices

Номер: US20130171628A1

Автор: Albert J. Mach, Dino Di Carlo, Soojung C. Hur

Принадлежит: UNIVERSITY OF CALIFORNIA

A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters ≧10 μm flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region.

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Номер записи: 25

18-07-2013 дата публикации

METHOD FOR HARVESTING EXPRESSION PRODUCTS

Номер: US20130183742A1

Автор: Leschke Christian, Wehnes Engelbert, Werner Uwe

Принадлежит: BAVARIAN NORDIC A/S

The present invention provides a method for recovering an essentially cell-associated expression product from a host cell comprising (a) culturing said host cell under conditions that allow expression of said expression product; (b) collecting said host cell in/on a filter unit; (c) disrupting said host cell in/on the filter unit; and (d) separating said expression product from said disrupted host cell. Said host cell is preferably a vertebrate cell, more preferably an avian cell, which is preferably cultured in suspension. Furthermore, the present invention provides for the use of a filter unit characterized in that said filter unit is (i) suitable to retain a host cell which expresses an expression product; and (ii) suitable for elution of said expression product from the filter unit after cell disruption in/on said filter unit for recovering said expression product from said host cell as well as for a system for recovering an expression product from a host cell comprising said filter unit. The present invention also provides an expression product obtainable by said method, said expression product being preferably a virus, specifically a poxvirus, in particular selected from the group consisting of fowlpoxvirus, vaccinia virus and, more preferably, modified vaccinia virus Ankara, MVA. 121-. (canceled)23. The method of claim 22 , wherein the poxvirus is selected from the group consisting of fowlpox virus claim 22 , vaccinia virus claim 22 , and modified vaccinia virus Ankara (MVA).24. The method of claim 23 , wherein the filter unit is suitable to separate the host cell from cell culture medium.25. The method of claim 23 , wherein the filter unit is suitable to allow passing through of the expression product and/or elution of the expression product from the host cell after cell disruption in or on the filter unit.26. The method of claim 25 , wherein the filter unit is further suitable to allow passing through of a disrupted host cell.27. The method of claim 23 , ...

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Номер записи: 26

01-08-2013 дата публикации

System and Process for Separating a Material

Номер: US20130196425A1

Автор: Chavarria Jason, DORIAN Randel, Leach Michael D., STORRS Richard W.

Принадлежит: Biomet Biologics, LLC

Disclosed is a system to separate, enrich, and/or purify a cellular population from a biological tissue, such as a tissue sample. For example, an adipose tissue sample can be acquired and disrupted. The disrupted tissue sample can then be separated and purified. The separated components can include multipotent, pluripotent, or other cell populations. 1. A system to separate a selected cell fraction from a sample volume , comprising:a container having a first wall extending from a first bottom wall to a an upper rim;a collection section positioned near the upper rim and at an exterior surface of the first wall, the collection section having a second wall extending from a second bottom wall wherein the second bottom wall is nearer the upper rim than the first bottom wall;a lid covering both the container and the collection section;wherein the lid is operable to move between an open position and a closed position to selectively close the container from the collection section.2. The system of claim 1 , further comprising:a drive motor operable to spin the container and the collection section simultaneously.3. The system of claim 1 , wherein the collection section at least directly contacts an exterior surface of the first wall.4. The system of claim 3 , wherein the upper rim of the container defines an annular section;wherein the collection section is an annular well surrounding the annular section.5. The system of claim 4 , further comprising:a sump defined within the collection section;wherein the selected cell fraction is operable to collect within the sump defined within the collection section.6. The system of claim 4 , further comprising:a barrier wall extending a width of the collection section;wherein the barrier wall defines a first end and a second end of the collection section;wherein a suction force can be applied at the first end to move the selected cell fraction within the collection section.7. The system of claim 1 , further comprising:a vent defined ...

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Номер записи: 27

22-08-2013 дата публикации

Acoustic waves in microfluidics

Номер: US20130213488A1

Автор: Achim Wixforth, Adam R. Abate, David A. Weitz, Jeremy Agresti, Lothar Schmid, Thomas Franke

Принадлежит: Harvard College

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.

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Номер записи: 28

22-08-2013 дата публикации

Bioreactor for Isolation of Rare Cells and Methods of Use

Номер: US20130216506A1

Автор: DISCHER DENNIS E., SHIN JAE-WON

Принадлежит: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

The present invention relates to a bioreactor apparatus, and methods of use, for the isolation of rare blood cells, including hematopoietic stem cells and megakaryocytes. The apparatus includes a soft substrate and an anti-contractility agent, thereby providing a soft microenvironment to cultured cells. The apparatus of the invention is permissive for the survival of non-dividing cells while dividing cells are eliminated. This unique property allows for the simple isolation of rare blood cells without the use of costly equipment and antibodies. 1. An apparatus for the isolation of non-dividing cells from a cell population comprising a substrate layer on at least one surface of the apparatus , wherein the substrate layer includes at least one composition comprising at least one anti-contractility agent for reducing cell contractility when a cell population is in contact with the substrate layer.2. The apparatus of claim 1 , wherein the at least one composition further comprises at least one compound selected from the group consisting of an aryl-hydrocarbon receptor antagonist and a growth factor.3. The apparatus of claim 1 , wherein the non-dividing cells are rare blood cells selected from the group consisting of hematopoietic stem cells claim 1 , polyploid megakaryocytes claim 1 , polyploid non-megakaryocytes claim 1 , granulocyte-macrophage progenitors claim 1 , and erythroid progenitors.4. The apparatus of claim 1 , wherein the substrate layer is a soft substrate having a low stiffness of about 0.3 kPa to 2 kPa.5. The apparatus of claim 1 , wherein the anti-contractility agent inhibits the function of myosin-II.6. The apparatus of claim 1 , wherein the at least one composition is embedded within the substrate layer.7. The apparatus of claim 1 , wherein the surface of the substrate layer is coated with at least one protein selected from the group consisting of collagen claim 1 , fibronectin claim 1 , laminin claim 1 , and vitronectin.8. A bioreactor for the ...

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Номер записи: 29

22-08-2013 дата публикации

Containers for agitation of liquid samples and methods of use thereof

Номер: US20130217010A1

Автор: Adam Suchocki, Charles William Rittershaus, Hwa-Tang Wang, James Franklin Chepin, Lori Anne Neely, Marilyn Lee Fritzemeier, Mark John Audeh, Matthew Blanco, Michael Min, Parrus Wellman, Thomas Jay Lowery, JR., Vasiliki Demas

Принадлежит: T2 Biosystems Inc

The present invention relates to containers for holding liquid samples. The containers may be useful for mixing a liquid sample or lysing cells in a liquid sample. The invention also relates to methods of using the containers of the invention.

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Номер записи: 30

26-09-2013 дата публикации

PROCESSOR UNIT FOR PROCESSING AND CONTROLLING A PREPARATION OF A BLOOD SAMPLE AND A METHOD

Номер: US20130252229A1

Автор: Holm Niels Erik

Принадлежит: VIVOSTAT A/S

Processor unit for processing and controlling a preparation of a blood sample placed in a preparation unit arranged in said processor unit comprising a piston placed in a first chamber for containing the blood sample. A part of the processed blood moves from the first chamber to a second chamber. The blood sample is centrifuged into separate layers comprising an outer layer adhering to the inner side of the outer first chamber wall and an inner layer placed opposite the outer layer. That the processor unit further comprises a first unit for emitting an outcome signal through the first chamber and a second unit for detecting after the signal has passed the first chamber an income signal. The piston is moved as a function of the detected income signal of the second unit. 1. Processor unit for processing and controlling a preparation of a blood sample , said processor unit comprising a preparation unit , the blood sample being placed in said processor unit ,said preparation unit comprisingSeveral chambers communicating with each other at predetermined steps under the processing of the blood sample,{'b': 1', '2, 'a piston placed in a first chamber for containing the blood sample, the first chamber comprises a cylindrical wall—an outer first chamber wall—and a bottom wall comprising of the piston and a top wall, said walls embracing the first chamber, in said first chamber the unprocessed blood sample is arranged, said rod of the piston is adapted to be moved coaxially with a centre axis of the preparation unit by first moving means from a first position d to a new position d different from the first position by said movement the volume of the first chamber is reduced whereby, a part of the processed blood moves from the first chamber to a second chamber placed below the bottom wall of the first chamber, said chambers being in fluid connection with each other,'}Said processor unit further comprises means for centrifugation and rotating the preparation unit around an axis ...

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Номер записи: 31

10-10-2013 дата публикации

Compositions and methods for adhesion-based cell sorting

Номер: US20130266943A1

Автор: Britani Blackstone, Heather Powell, John Lannutti

Принадлежит: Individual

In an aspect, provided is an apparatus for sorting cells. The device may include polymer nanofibers treated with gaseous plasma. The nanofibers may comprise at least one of polycaprolactone and collagen. The gaseous plasma may comprise at least one of CF 4 , oxygen, argon, nitrogen, and air. In a further aspect, provided are methods of sorting cells in a composition. The method may include providing a substrate comprising polymer nanofibers that have been pretreated with a gaseous plasma, contacting the polymer nanofibers with a composition comprising a plurality of cells, and applying a force to the polymer nanofibers. In another aspect, provided are methods of making a device for sorting cells. The method may include applying a composition comprising at least one polymer onto a surface by electrospinning to form polymer nanofibers, and exposing the polymer nanofibers to a gaseous plasma to produce treated polymer nanofibers.

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Номер записи: 32

31-10-2013 дата публикации

Cell collecting device

Номер: US20130288360A1

Автор: Byung Hee Jeon, Jong Kil Lee

Принадлежит: Cytogen Inc

A cell collecting device is configured to collect target cells from a fluid sample such as blood or physiological fluid. The cell collecting device includes a first filter having a plurality of first pores formed into a size that enables target cells contained in a fluid sample to pass through the first pores, and a second filter having a plurality of second pores formed into a size that enables the target cells contained to pass through the second pores. The second filter is arranged below the first filter in such a position as to filter the target cells.

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Номер записи: 33

21-11-2013 дата публикации

Method and apparatus for producing cells and fat soluble materials by cell culture

Номер: US20130309757A1

Автор: Sung-Chun Kim

Принадлежит: Individual

The present invention relates to a method and apparatus for producing cells without injury and fat-soluble materials by from cell culturing in an inexpensive and highly efficient manner. The apparatus according to the present invention comprises a culturing device 10 , a solvent device 20 , a mixing device 30 , a separation device 40 , a fractionation device 50 , a cell accommodation device 60 , and a fat-soluble material solvent accommodation device 70.

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Номер записи: 34

26-12-2013 дата публикации

DEVICE FOR SEPARATING ADULT STEM CELL

Номер: US20130344589A1

Автор: MATTHIESEN Inge, WINKLER Konrad-Wenzel

Принадлежит:

The invention relates to a device () for separating adult stem cells from adipose tissue taken from a biological structure. The device () has a container () for receiving a material mixture with the adipose tissue and the adult stem cells. Furthermore, the device () has a rinse agent feed device (), a mixture feed device (), a stem cell extraction device (), a rinse agent drainage device (), and a selectively permeable membrane (-). The container () has at least two chambers (-) which are separated from one another by at least one membrane (-). The mixture feed device () and the stem extraction device () are also separated by the at least one membrane (-). 1100200. A device ( , ) for separating adult stem cells from adipose tissue taken from a biological structure , comprising:{'b': '110', 'a container () for receiving a material mixture comprising the adipose tissue and adult stem cells;'}{'b': 120', '330', '110, 'at least one rinse agent feed device () configured to introduce a fluid () into the container ();'}{'b': 130', '110, 'a mixture feed device () configured to introduce the mixture into the container ();'}{'b': 140', '110, 'a stem cell extraction device () configured to remove the separated stem cells from the container ();'}{'b': 150', '110, 'at least one rinse agent drainage device () configured to discharge a mixture reduced by adult stem cells from the container (), and'}{'b': 161', '165, 'at least one selectively permeable membrane (-), which is permeable to only a specific part of the respective material mixture,'}wherein{'b': 110', '171', '174', '161', '165, 'the container () comprises at least two chambers (-) which are separated from one another by at least one membrane (-), and'}{'b': 130', '140', '161', '165, 'the mixture feed device () and the stem cell extraction device () are separated from one another by the at least one membrane (-).'}2171174181183. The device of claim 1 , wherein the at least two chambers (-) comprise at least one pressure ...

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Номер записи: 35

09-01-2014 дата публикации

Sample probes and methods for sampling intracellular material

Номер: US20140011226A1

Автор: Kristin Briana Bernick, Nicholas M. Sampas

Принадлежит: AGILENT TECHNOLOGIES INC

A sample probe includes a tip including a distal end for penetrating a cellular membrane, an opening located at or proximal to the distal end, and tip microchannels extending through the tip and communicating with the opening; and a body adjoining the tip and including body microchannels, wherein at least one of the body microchannels communicates with at least one of the tip microchannels. A method for sampling intracellular material includes inserting a probe tip through a cellular membrane; aspirating intracellular material from the cell, through an opening of the tip, and into a first microchannel of the tip; flowing isolator fluid from a second microchannel of the tip into the first microchannel to form a plug of intracellular material; and aspirating the plug and the isolator fluid through the first microchannel.

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Номер записи: 36

16-01-2014 дата публикации

RECTANGULAR CHANNEL ELECTRO-ACOUSTIC AGGREGATION DEVICE

Номер: US20140017759A1

Автор: Kale Aniket, KNIEP Justin S.

Принадлежит:

Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field and/or acoustic energy to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary. 1. An apparatus for aggregating microorganisms in an aqueous suspension , comprising:a. at least one first electrical conductor configured as a cathode;b. at least one second electrical conductor configured as an anode;c. at least one pair of spaced insulators disposed between the at least one first electrical conductor and the at least one second electrical conductor, the at least one first conductor being disposed opposite the at least one second electrical conductor, such that a channel is defined between the at least one first electrical conductor, the at least one second electrical conductor, and the at least one pair of spaced insulators, the channel providing a fluid flow path for the aqueous suspension;d. an electrical power source operably connected to the at least one first electrical conductor and the at least one second electrical conductor, wherein an electrical field is created by providing an electric current from the electrical power source to the at least one first electrical conductor and the at least one second electrical conductor;e. at least one first transducer coupled to the at least one first electrical conductor;f. at least one second transducer coupled to the at least one second electrical conductor; andg. a generator configured to produce and transmit electrical radio frequency signals, wherein the generator transmits an electrical radio frequency signal to the at least one first and second transducers, and the transducers convert the electrical signal into an ...

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Номер записи: 37

23-01-2014 дата публикации

Use of oxyhydrogen microorganisms for non-photosynthetic carbon capture and conversion of inorganic and/or c1 carbon sources into useful organic compounds

Номер: US20140024091A1

Автор: John S. Reed, Lisa Dyson

Принадлежит: Kiverdi Inc

Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or C1 carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing C1 chemicals including but not limited to various syngas compositions, into organic chemicals including biofuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or C1 carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO 2 in one or more steps of the process.

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Номер записи: 38

30-01-2014 дата публикации

Methods and systems for harvesting cells

Номер: US20140030805A1

Автор: Barakf Zohar, Harel KASUTO, Nirit Drori-Carmi

Принадлежит: Pluristem Ltd

Methods for using vibration to harvest cells grown in 3 D culture are provided. The methods entail the application of force to cells attached to a 3 D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.

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Номер записи: 39

20-02-2014 дата публикации

Methods and Devices to Control Fluid Volumes, Reagent and Particle Concentration in Arrays of Microfluidic Drops

Номер: US20140051062A1

Автор: Bithi Swastika S., Sun Meng, Vanapalli Siva A.

Принадлежит: Texas Tech University System

The present invention includes a microfluidic device comprising one or more parking loops , each parking loop comprising a bypass channel and a lower branch capable of retaining one or more drops, wherein bypass channel has a smaller hydrodynamic resistance than the lower branch 1. A microfluidic device comprising:a substrate comprising an inlet and an outlet connected to a main conduit;{'b': 12', '12', '14', '16', '14', '16', '16, 'one or more parking loops connected to the main conduit, each parking loop comprising a bypass channel and a lower branch with a fluidic trap capable of retaining one or more drops, wherein the bypass channel has a smaller hydrodynamic resistance than the lower branch with a fluidic trap .'}216. The device of claim 1 , further comprising a cartridge connected to the inlet claim 1 , wherein a sample slug can be introduced into the main conduit and into the fluidic trap .3. The device of claim 1 , wherein a hydrodynamic resistance ratio between the bypass channel and the lower branch is from 1.0 to 2.0 claim 1 , wherein the bypass channel has a smaller hydrodynamic resistance than the lower branch.4. The device of claim 1 , wherein a hydrodynamic resistance ratio between the bypass channel and the lower branch is from 1.4 to 1.6 claim 1 , wherein the bypass channel has a smaller hydrodynamic resistance than the lower branch.5. The device of claim 1 , wherein the microfluidic device further comprises a fluid that is at least partially aqueous.6. The device of claim 1 , wherein the device comprises an array of parking loops formed into at least one of a square array claim 1 , a triangular array claim 1 , a pentagonal array claim 1 , a hexagonal array claim 1 , a rectangular array claim 1 , a polygonal array claim 1 , a circular array claim 1 , an oval array claim 1 , an undular array claim 1 , or a three-dimensional array.7. The device of claim 1 , wherein a reagent drop is introduced into the bypass channel to control the passage of one or ...

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Номер записи: 40

20-02-2014 дата публикации

Concentrating Components of Fluid Circulated through a Cell Growth Chamber

Номер: US20140051167A1

Автор: DiLorenzo Thomas G., Jones Mark E., KINZIE Michael E., NANKERVIS Brian J.

Принадлежит: TERUMO BCT, INC.

One or more embodiments are described directed to a method and system for concentrating components of a fluid circulated through a cell growth chamber such as a cell growth chamber. Accordingly, embodiments include methods and systems that utilize a tangential flow filter to concentrate components of a fluid that in embodiments includes expanded cells. In embodiments, a concentrated fluid component and a concentrated cell component are generated by flowing the fluid with expanded cells across a tangential flow filter. The concentrated cell component may be recirculated to the tangential flow filter to reach some desired concentration of cells. The concentrated fluid component may be collected to utilize cellular-produced constituents in the concentrated fluid component. 1. A method of concentrating a component from a fluid circulated in a cell growth chamber , the method comprising:expanding cells within a cell growth chamber;removing expanded cells from the cell growth chamber using a first fluid;circulating the first fluid with the expanded cells across a tangential flow filter that is fluidly associated with the cell growth chamber to generate a concentrated fluid component and a concentrated cell component; andcollecting at least a portion of the concentrated fluid component in a container.2. (canceled)3. The method of claim 1 , further comprising;recirculating the concentrated cell component across the tangential flow filter to concentrate the cells in the concentrated cell component.4. The method of claim 1 , further comprising:before circulating the first fluid across the tangential flow filter, adding a second fluid to the first fluid.5. The method of claim 3 , further comprising:before recirculating the concentrated cell component across the tangential flow filter, adding a second fluid to the concentrated cell component.6. The method of claim 1 , wherein the cell growth chamber and the tangential flow filter are part of a closed system.7. The method of ...

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Номер записи: 41

13-03-2014 дата публикации

Microstructure for Particle and Cell Separation, Identification, Sorting, and Manipulation

Номер: US20140072952A1

Автор: George Hvichia

Принадлежит: Parsortix Inc

The invention relates to microscale cell separating apparatus which are able to separate cells on the basis of size of the cells, interaction of the cells with surfaces of the apparatus, or both. The apparatus comprises a stepped or sloped separation element ( 16 ) interposed between an inlet region ( 20 ) and an outlet region ( 22 ) of a void that can be tilled with fluid. The void can be enclosed within a cover ( 12 ) and fluid flow through the void engages cells with the separation element. Only cells which have (or can deform to have) a characteristic dimension smaller than or equal to the distance between a step and the cover or body can pass onto or past a step. Modifications of surfaces within the apparatus can also inhibit passage of cells onto or past a step.

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Номер записи: 42

20-03-2014 дата публикации

ULTRASOUND AND ACOUSTOPHORESIS FOR COLLECTION AND PROCESSING OF OLEAGINOUS MICROORGANISMS

Номер: US20140080207A1

Автор: Carmichael Joey, Dionne Jason, Lipkens Bart, Mealey Dane, Mitchell Eric

Принадлежит: FloDesign Sonics, Inc.

Microorganisms such as microalgae are collected and separated from a host medium such as water. Cellular walls and membranes of the microorganisms are then ruptured to release their lipids using a lipid extraction unit. Thereafter, the lipids from the host medium are collected and separated using a lipid collection and separation unit. Related apparatus, systems, techniques and articles are also described. 120-. (canceled)21. An apparatus comprising: a first inlet, a primary outlet, and a secondary outlet, wherein an initial mixture of a host fluid and microorganisms enters through the first inlet;', 'at least one first ultrasonic transducer; and', 'a reflector surface opposite the at least one first ultrasonic transducer;', 'wherein the at least one first ultrasonic transducer operates to form a standing acoustic wave substantially perpendicular to the flow of the initial mixture to selectively separate the microorganisms from the host fluid, such that a majority of the host fluid exits the first flow chamber via the primary outlet and the microorganisms and residual host fluid exit the first flow chamber via the secondary outlet;, 'a microorganism collection and separation unit comprising a first flow chamber, the first flow chamber comprising a second inlet through which is flowed the mixture of microorganisms and residual host fluid from the secondary outlet of the first flow chamber;', 'a primary outlet; and', 'at least one second ultrasonic transducer forming a standing acoustic wave to selectively rupture cellular walls and membranes of the microorganisms and form cellular debris, the residual host fluid and cellular debris exiting the second flow chamber via the primary outlet; and, 'operatively connected to a secondary outlet of the first flow chamber, an extraction unit comprising a second flow chamber, the second flow chamber comprising a third inlet through which is flowed the mixture of residual host fluid and cellular debris;', 'a primary outlet and a ...

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Номер записи: 43

27-03-2014 дата публикации

METHODS AND DEVICES FOR SAMPLE LYSIS

Номер: US20140087359A1

Автор: Gorman John, Maltezos George, NJOROGE Samuel, Scherer Axel

Принадлежит: California Institute of Technology

Disclosed herein are methods and systems for use in preparing a sample. The methods and systems may be used for lysing one or more structures in a sample (e.g., cells, viral particles, etc.). The methods and compositions may comprise a microfluidic chip or use thereof. The microfluidic chips disclosed herein may comprise (a) a substrate comprising a chamber, wherein at least one mechanical element may be located within the chamber; (b) a thermal element in contact with the chamber; and (c) at least one aperture within the surface of the substrate, wherein the aperture may be configured to insulate the chamber. 1. A microfluidic chip comprising:a. a chamber, wherein at least one stir bar is located within the chamber;b. a thermal device in thermal contact with the chamber; andc. at least one insulative groove within the surface of the chip, which is configured to insulate the chamber.2. A microfluidic chip comprising:a. a chamber, wherein a plurality of granular particles is located within the chamber;b. a thermal device in thermal contact with the chamber; andc. at least one insulative groove within the surface of the chip, which is configured to insulate the chamber.3. The microfluidic chip of or , wherein the thermal device is a heater.4. The microfluidic chip of or , wherein the thermal device is a cooler.5. The microfluidic chip of or , wherein the at least one insulative groove is nine insulative grooves.6. The microfluidic chip of or , wherein the at least one insulative groove is a plurality of grooves positioned around the chamber.7. The microfluidic chip of or , wherein the at least one insulative groove is filled with an insulative material.8. The microfluidic chip of claim 1 , wherein the stir bar is composed of magnetic material.9. The microfluidic chip of or claim 1 , wherein the microfluidic chip is coupled to an external magnetic field.10. The microfluidic chip of claim 2 , wherein the granular particles are beads.11. The microfluidic chip of claim 10 ...

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Номер записи: 44

10-04-2014 дата публикации

CELL-ADHERING LIGHT-CONTROLLABLE SUBSTRATE

Номер: US20140099695A1

Автор: FURUTA Toshiaki, Ozawa Satoshi, Sugiyama Hisashi, SUZUKI Akinobu, TADA Hiroko

Принадлежит:

An object of the present invention is to enable simpler operation in real time and culture while removing unnecessary cells from cultured cells for purification in analyzing, fractionating, and culturing the cells alive and to analyze and fractionate desired cells from the cultured cells to increase the purity, recovery rate, and viability of the cells. The present invention employs a cell-adhesive photocontrollable base material, wherein light irradiation causes the bond dissociation of a photolabile group comprising a coumarinylmethyl skeleton to produce the separation of a cell-adhesive material to leave a non-cell-adhesive material. As a result, cell images can be detected and analyzed to obtain the positional information of desired cells. Based on the positional information thus obtained, the cells can be analyzed and fractionated alive. 1. A cell-adhesive photocontrollable base material , wherein light irradiation causes the bond dissociation of a photolabile group comprising a coumarinylmethyl skeleton to separate a cell-adhesive material and leave a non-cell-adhesive material on a base material.2. A cell-adhesive photocontrollable base material obtained by conducting a film formation of a cell-adhesive photocontrollable material on the base material , wherein a cell-adhesive material binds to a non-cell-adhesive material via a photolabile group comprising a coumarinylmethyl skeleton in the cell-adhesive photocontrollable material in which the non-cell-adhesive material , the photolabile group and the cell-adhesive material are bound in this order from the base material side. to3. A cell-adhesive photocontrollable base material , wherein light irradiation causes the bond dissociation of a photolabile group comprising a coumarinylmethyl skeleton to irreversibly change the surface of the irradiated portion from the cell-adhesive material to the non-cell-adhesive material.12. The cell-adhesive photocontrollable base material according to claim 1 , wherein the ...

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Номер записи: 45

10-04-2014 дата публикации

DEVICES AND METHODS FOR CELL LYSIS AND SAMPLE PREPARATION THROUGH CENTRIFUGATION

Номер: US20140100102A1

Автор: ALLENDORPH CARL J., CHEN Samson, KARTALOV Emil P., RAJAGOPAL Aditya, Scherer Axel

Принадлежит: California Institute of Technology

Methods and devices for molecular analysis are disclosed, based on centrifugation. A centrifuge device comprises strips of centrifuge tubes and elements to create a magnetic field. The magnetic shear forces applied to beads inside a solution with biological molecules permit the performance of different analytic techniques, such as lysis and sample preparation for PCR. 1. A centrifuge device comprising:a rotating head;at least one centrifuge tube;at least one slot in the rotating head, configured to accept the at least one centrifuge tube; andat least one field element in the rotating head, wherein the at least one field element is configured to generate a magnetic field.2. The centrifuge device of claim 1 , wherein the at least one centrifuge tube has a filter configured to allow disposal of waste products claim 1 , while retaining desired products.3. The centrifuge device of claim 2 , wherein the desired products comprise chemically- and/or biologically-functionalized beads claim 2 , and magnetic beads claim 2 , wherein the functionalized beads are configured to attach to desired cells.4. The centrifuge device of claim 3 , wherein the functionalized beads and the magnetic beads are configured to selectively and/or blindly capture the desired cells.5. The centrifuge device of claim 3 , wherein the desired cells comprise nucleic acid within walls and/or membranes of the desired cells.6. The centrifuge device of claim 5 , wherein the desired cells are for lysis or polymerase chain reaction.7. The centrifuge device of claim 2 , further comprising at least one receptacle attached to a bottom end of the at least one centrifuge tube claim 2 , wherein the at least one receptacle is for storing the waste products.8. The centrifuge device of claim 1 , further comprising at least one receptacle attached to a bottom end of the at least one centrifuge tube.9. The centrifuge device of claim 8 , wherein the at least one receptacle is for storing eluted nucleic acid products.10. ...

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Номер записи: 46

06-01-2022 дата публикации

Cell therapy vessels

Номер: US20220002653A1

Автор: Alden LADD

Принадлежит: Bluebird Bio Inc

The present disclosure provides improved cell therapy vessels, cell therapy vessel adapters, kits, and methods of using the same.

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Номер записи: 47

06-01-2022 дата публикации

CLOSED SYSTEM DEVICE AND METHODS FOR GAS PERMEABLE CELL CULTURE PROCESS

Номер: US20220002659A1

Автор: Welch Daniel P., Wilson John R.

Принадлежит:

Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices. 1. A method of removing media and cells from a cell culture device , the method comprising: a gas delivery component,', 'a first fluid detection component that is capable of determining when a fluid moving within a media removal conduit connected to a cell culture device changes from a liquid to a gas, said first fluid detection component capable of sending a signal to a first fluid flow control component that is capable of terminating the fluid flow through the media removal conduit,', 'a second fluid detection component that is capable of determining when the fluid moving within a cell removal conduit that is connected to the cell culture device changes from liquid to gas, said second fluid detection component capable of sending a signal to a second fluid flow control component that is capable of terminating the fluid flow through the cell removal conduit; said cell culture device comprising a gas permeable bottom upon which cells reside, a media removal conduit, a cell removal conduit, and a filter;, 'using a device to reduce a volume of a liquid media in the cell culture device, said device comprising'}increasing the concentration of cells per milliliter of media within said cell culture device by connecting said gas delivery component to said filter,connecting said first fluid detection component to said media removal conduit,connecting said first fluid flow control component to said media removal conduit,initiating gas delivery from said gas delivery component, whereby gas moves into said cell culture device and displaces media from said cell culture device into a media collection vessel connected to said media removal conduit, said first ...

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Номер записи: 48

06-01-2022 дата публикации

INFECTIOUS DISEASE SCREENING DEVICE

Номер: US20220002823A1

Автор: Alshaiba Saleh Ghannam Almazrouei Mohammed, Bhatti Sajid, Lahoud Imad, Lamoureux Clement, Machovec Jeff

Принадлежит:

A disease screening device () comprising a substrate () and a sonication chamber () formed on the substrate (). The sonication chamber () is provided with an ultrasonic transducer () which generates ultrasonic waves to lyse cells in a sample fluid within the sonication chamber (). The device () comprises a reagent chamber () formed on the substrate () for receiving a liquid PCR reagent. The device () comprises a controller () which controls the ultrasonic transducer () and a heating arrangement () which is provided on the substrate (). The device () further comprises a detection apparatus which detects the presence of an infectious disease, such as COVID-19 disease. 1. A COVID-19 disease screening device comprising:a substrate which is at least partly composed of silicon, the substrate comprising a sonication chamber having a sample inlet and a sample outlet;an ultrasonic transducer in ultrasonic communication with the sonication chamber, wherein the ultrasonic transducer generates ultrasonic waves in a frequency range of approximately 2800 kHz to approximately 3200 kHz to lyse cells in a sample fluid within the sonication chamber; an AC driver which generates an AC drive signal at a frequency within the frequency range of approximately 2800 kHz to approximately 3200 kHz and outputs the AC drive signal to drive the ultrasonic transducer;', 'an active power monitor which monitors active power used by the ultrasonic transducer when the ultrasonic transducer is driven by the AC drive signal, wherein the active power monitor provides a monitoring signal which is indicative of the active power used by the ultrasonic transducer;', 'a processor which controls the AC driver and receives the monitoring signal from the active power monitor; and', A. control the AC driver to output the AC drive signal to the ultrasonic transducer at a frequency within the frequency range;', 'B. calculate the active power being used by the ultrasonic transducer based on the monitoring signal;', ...

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Номер записи: 49

05-01-2017 дата публикации

DEVICE AND KIT FOR PREPARING TISSUE, PARTICULARLY ADIPOSE TISSUE, FOR TRANSPLANTATION FROM LOBULAR FAT EXTRACTED BY LIPOSUCTION

Номер: US20170000969A1

Автор: Tremolada Carlo

Принадлежит:

A device for preparing adipose tissue for transplantation from lobular fat extracted, for instance by liposuction, said fat consisting of a fluid component comprising an oily component, a blood component and/or sterile solutions and a solid component comprising cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, comprising at least one washing and separating container () having a washing chamber () for washing the liposuctioned material, which container () has an inlet () and an outlet () for the liposuctioned material to enter the washing chamber () through the inlet () and for at least part of said material, particularly the fluid component, to exit said chamber () through the outlet (), said washing chamber () including means for forming an emulsion of fluid components, by mechanical stirring. 1. A device for preparing adipose tissue for transplantation from lobular fat material extracted by liposuction , said fat material comprising (a) a fluid component comprising one or more of an oily component , a blood component and sterile solutions , and (b) a solid component comprising cell fragments , cells and one or more cell macroagglomerates of heterogeneous size , said device comprising:at least one washing and separating container having a washing chamber for washing the liposuctioned material, said container having at least one opening defining an inlet and an outlet for the liposuctioned material to enter the washing chamber through the inlet and for at least part of said liposuctioned material to exit said chamber through the outlet, said washing chamber including an emulsion-forming means for forming an emulsion of fluid components by mechanical stirring; anda size reducing means provided in the washing and separating container or in a size reducing container adapted to be fluid-tightly connected to said washing and separating container and configured for reducing the size of the solid component to, on average, equal smaller ...

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Номер записи: 50

07-01-2021 дата публикации

SYSTEM AND METHOD FOR DETECTION AND SORTING OF CELLS

Номер: US20210001339A1

Автор: Filatov Zerikhun, Liu Peng

Принадлежит: Microsensor Labs, LLC

A system and method for detection of cells and sorting of cells are disclosed. Target cells, such as circulating tumor cells (CTCs) or antigen-specific antibody producing circulating memory B cells from COVID-19 patients, may be of interest. Magnetic beads may be bound to the target cells. After which, the bead-bound target cells may be identified using an applied magnetic field. In one example, magnetic sensors may be used to detect movement of the bead-bound target cells responsive to an applied magnetic field. In another example, an optical sensor may be used to detect movement of the bead-bound target cells responsive to an applied magnetic field. Further, separate from identification of the target cells, the bead-bound target cells may be sorted using an applied magnetic field. In this way, a magnetic field may be used for target cell identification and target cell sorting in order to detect and collect target cells of interest at the single-cell resolution. 1. An apparatus configured to determining whether a magnetic bead-labeled target cell is present in a fluid , the apparatus comprising:a well configured to house the fluid containing particles and including at least one outlet;at least one magnetic field generator configured to generate a magnetic field to at least a part of the well;one or more sensors configured to generate sensor data; and control the magnetic field generator to generate the magnetic field to the at least a part of the well;', 'identify, based on the sensor data responsive to the magnetic field, the magnetic bead-labeled target cell and an associated location within the well; and', 'control the magnetic field generator, based on the associated location within the well of the magnetic bead-labeled target cell and the at least one outlet, in order to move the magnetic bead-labeled target cell toward the at least one outlet, thereby sorting the magnetic bead-labeled target cell, in order to remove the magnetic bead-labeled target cell from ...

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Номер записи: 51

03-01-2019 дата публикации

Devices And Method For Enrichment And Alteration Of Cells And Other Particles

Номер: US20190001344A1

Автор: Barber Thomas A., Carvalho Bruce L., Huang Lotien Richard, Kapur Ravi, Toner Mehmet, Vernucci Paul, Wang Zihua

Принадлежит:

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample. 1150-. (canceled)151. A method of enriching a sample in fetal cells relative to maternal cells , the method comprising:(a) introducing a maternal blood sample into a microfluidic device capable of enriching fetal nucleated cells relative to maternal cells based on size, shape, deformability, or affinity to produce an enriched sample;(b) lysing fetal nucleated cells in the enriched sample to release fetal nuclei; and(c) detecting fetal nuclei.152. The method of claim 151 , wherein step (b) comprises lysing all cells in the enriched sample and collecting fetal nuclei.153. The method of claim 151 , wherein step (b) comprises selectively lysing fetal nucleated cells relative to maternal cells.154. The method of claim 151 , wherein the microfluidic device comprises a channel having a structure that deterministically directs fetal nucleated cells in a first direction and at least some maternal cells in a second direction based on deterministic lateral displacement.155. The method of claim 154 , wherein the microfluidic device is a duplex device comprising a channel comprising a first section comprising first and second outer regions claim 154 , each outer region comprising a structure that deterministically directs particles ...

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Номер записи: 52

07-01-2016 дата публикации

Megakaryocyte and Platelet Production from Stem Cells

Номер: US20160002586A1

Автор: Mitchell Beau W.

Принадлежит:

Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein. 1. A platelet production system for the ex vivo production of platelets comprising:a fluid source, the fluid comprising a growth medium;a platelet production chamber in fluid communication with the fluid source, the chamber comprising a first and a second fluid flow path, the first and second fluid flow paths separated from each other within the chamber by a permeable scaffold;wherein the permeable scaffold is configured to allow a plurality of megakaryocytes located in the first fluid path to extend their respective proplatelet processes through the permeable scaffold toward the second fluid flow path;wherein the platelet production system is configured to transport fluid from the fluid source into the platelet production chamber; andwherein the second fluid flow path brings the growth medium into contact with at least a portion of the permeable scaffold to remove at least some platelets from the proplatelet processes.2. The platelet production system of further comprising a platelet collection chamber.3. The platelet production system of claim 1 , wherein the growth medium flows through the first platelet production chamber at a volumetric flow rate of between about 15 ml/min and about 55 ml/min.4. The platelet production system of claim 1 , wherein the growth medium exhibits a shear rate at an interface with the permeable scaffold that is between about 10 sand about 100 s.5. The platelet production system of claim 1 , wherein the growth medium exhibits a shear rate at an interface with the permeable scaffold that is at least about 30 s.6. The platelet production system of claim 1 , wherein the growth medium exhibits a shear rate at an interface with the permeable scaffold that is less than about 70 s.7. The platelet production system of claim 1 , wherein the permeable scaffold has a thickness of between about 100 μm and about 200 μm.8. ...

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Номер записи: 53

07-01-2016 дата публикации

DEVICE AND METHODS FOR PLATELET LYSIS OR ACTIVATION

Номер: US20160002598A1

Автор: Centeno Christopher J., Dregalla Ryan, Leach Brian J., Lyons Nicolette, Reischling Patrick

Принадлежит: Regenerative Science, LLC

Device, system and method embodiments are disclosed herein which provide for the production of a modified autologous platelet solution at the patient bedside for contemporaneous reinjection to the patient. In certain embodiments all of the steps including, but not limited to blood draw, platelet lysis, solution preparation and reinjection to a patient may be accomplished in a single office or clinic visit without relocating the patient. Accordingly, the apparatus, devices and systems disclosed herein generally include a substantially stand-alone machine, device or system which is configured to accept a platelet containing solution, induce lysis of one or more platelet bodies within the platelet containing solution and provide the resulting modified platelet solution in a manner suitable for injection into the patient. 1. A device comprising:a housing;an input port into the housing providing for the input of a platelet containing solution;a lysis/activation chamber in fluid communication with the input port and positioned within the housing, wherein one or more platelets within the platelet containing solution input to the device at the input port are caused to undergo lysis or activation in the lysis/activation chamber, thereby creating modified solution; andan outlet port from the housing, in fluid communication with the lysis/activation chamber, providing for the modified solution to be removed through the outlet port.2. The device of further comprising a heating and cooling module in thermal communication with the lysis/activation chamber claim 1 , said heating and cooling module providing for the thermal lysis or activation of one or more platelets of the platelet containing solution within the lysis/activation chamber.3. The device of wherein the lysis/activation chamber comprises a length of disposable sterile tubing.4. The device of wherein the heating and cooling module causes the platelet containing solution to wholly or partially freeze and wholly or ...

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Номер записи: 54

07-01-2016 дата публикации

Methods and Apparatus for Cell-Free Microfluidic-Assisted Biosynthesis

Номер: US20160002611A1

Автор: Gershenfeld Neil A., Mershin Andreas, Noireaux Vincent, Pelletier James Francis

Принадлежит:

A trans-disciplinary system for cell-free biosynthesis includes a cell-free transcription-translation (TX-TL) tool and modular, generalizable microfluidic architectures. Both components of the system are independently functional and are combinable into a cell-free biosynthesis platform. In the first component, modular plasmid libraries are used to program bacterial cell-free TX-TL systems. Each plasmid holds one gene or operon, and all the genes are controlled by the same promoter, so that the stoichiometry of enzyme synthesis is determined by the stoichiometry of plasmids in the reaction. In the second part, in order to facilitate high throughput mixing and matching of gene units from the modular plasmid libraries, a modular, reconfigurable, flexible, and scalable microfluidic architecture is employed. The microfluidic modules share common form factors and port/valve locations, so that a small set of module types, with multiple instances of each type interconnected in different geometries, allows simple reconfiguration to achieve different modes of operation. 1. A method for cell-free synthesis of a biosynthetic product , comprising the steps of: preparing a selected bacterial cell culture;', 'generating a cell extract from the bacterial cell culture;', 'combining the cell extract with amino acid and energy solutions to create a transcription-translation reaction buffer; and', 'separating reaction buffer aliquots by adding enzyme genes;, 'performing cell-free transcription-translation by the steps ofinfusing a microfluidic device with the reaction buffer aliquots, wherein the microfluidic device comprises one or more microfluidic modules configured for generating, controlling, and manipulating droplets of the reaction buffer aliquots;generating, controlling, and manipulating the droplets according to the configuration of the microfluidic device; andextracting droplets containing target molecules from the microfluidic device.2. The method of claim 1 , wherein the ...

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Номер записи: 55

05-01-2017 дата публикации

DEVICE FOR SEPARATING CELLS IN FLUID

Номер: US20170002308A1

Автор: Liu Tao

Принадлежит:

The present disclosure provides a device for separating cells in fluid, comprising a first driving pump, a separation column, a detection column, a second driving pump, and several three-way valves and secondary driving pumps; one end of the separation column is connected with the first driving pump through a first three-way valve; the other end is connected with the second driving pump through a second three-way valve; the separation column includes at least five sub filtration columns in parallel; the sub filtration column comprises a fixing bracket and a track-etched membrane of polycarbonate or polyester material attached to the bottom of and the side surfaces all around the fixing bracket, wherein the pore diameter of the track-etched membrane is 5-25 μm. The device of the present disclosure not only provides a new method for accurately determining whether there are circulating tumor cells in the blood of the living animal and provides a new method for sorting and counting the circulating tumor cells in the blood of the living animal, but also provides a pioneering new method and new device for therapy of the tumor transfer and removal of the tumor cells inside the body, and has extremely high economic and social values. 1. A device for separating cells in fluid , comprising a first driving pump communicating with an sample injection port , a separation column , a detection column , a second driving pump for driving cells separated from the separation column to enter the detection column , and several three-way valves as well as secondary driving pumps ,wherein one end of the separation column is connected with the first driving pump through a first three-way valve, the other end of the separation column is connected with the second driving pump through a second three-way valve, the detection column is connected with a third channel port of the first three-way valve, and a third channel port of the second three-way valve is connected with a sample discharge ...

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Номер записи: 56

05-01-2017 дата публикации

METHOD FOR PREPARING TISSUE, PARTICULARLY ADIPOSE TISSUE, FOR TRANSPLANTATION FROM LOBULAR FAT EXTRACTED BY LIPOSUCTION

Номер: US20170002323A1

Автор: Tremolada Carlo

Принадлежит:

A method of preparing adipose tissue for transplantation, from lobular fat extracted, for instance, by liposuction, said fat consisting of a fluid component comprising an oily component, a blood component and/or sterile solutions and of a solid component comprising cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, characterized in that it includes at least one step of washing the cell aggregates carried out at the same time as a step of separating the fluid component, in emulsion form, from the solid component. 1. A method of preparing adipose tissue for transplantation from lobular fat material extracted from a body by liposuction , said fat material comprising (a) a fluid component comprising one or more of an oily component , a blood component and sterile solutions , and (b) a solid component comprising cell fragments , cells and one or more cell macroagglomerates of heterogeneous size , said method comprisingwashing the solid component at least once, and separating the fluid component, in emulsion form, from the solid component, wherein said washing and separating steps are carried out at the same time, and wherein said washing and separating steps include:injecting at least one sterile washing solution into a washing chamber containing the fat material, of a washing and separating container, wherein the washing chamber contains an outlet and at least one stirring element;manually or mechanically stirring said washing and separating container to facilitate the emulsion of the fluid components;arranging the washing and separating container in a vertical position relative to the ground, with the outlet facing downwards, to obtain a stratification of the solid components on the liquid emulsion, which constitutes the fat material contained in the washing chamber, which results in a solid component, composed of cell fragments, cells and one or more cell agglomerates, floating on an emulsion of the fluid components that are present in ...

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Номер записи: 57

04-01-2018 дата публикации

METHOD AND MATERIAL FOR SEPARATING LEUKOCYTES OR MONONUCLEAR CELLS

Номер: US20180002663A1

Автор: Sato Nobuhiko, Tsukamoto Ayako

Принадлежит: KANEKA CORPORATION

An object of the present invention is to provide a separation system and a separation material for efficiently separating white blood cells or mononuclear cells from a biological fluid containing blood cell components. White blood cells or mononuclear cells can be efficiently separated by contacting a biological fluid containing blood cell components with a separation material having an average fiber diameter of at least 2.0 μm but not more than 6.0 μm and an air permeability coefficient M of at least 6.2 but not more than 35 to capture white blood cells on the separation material, and recovering the captured white blood cells or mononuclear cells using a detachment solution. 122-. (canceled)23. A cell separation method , comprising:a step of contacting a biological fluid comprising white blood cells and/or mononuclear cells with a separation material,wherein the separation material captures 60% or more of the white blood cells and/or the mononuclear cells from the biological fluid,{'sup': '2', 'wherein the separation material comprises a nonwoven fabric having an average fiber diameter of at least 2.0 μm, but not more than 6.0 μm, and an air permeability coefficient M of at least 7.0 but not more than 14.2 (cc/cm·sec)mm,'}wherein the biological fluid comprises at least one selected from the group consisting of peripheral blood, umbilical cord blood and bone marrow.24. The cell separation method according to claim 23 , wherein the captured white blood cells or mononuclear cells comprise hematopoietic stem cells and/or mesenchymal stem cells.25. The cell separation method according to claim 24 , wherein the hematopoietic stem cells and/or mesenchymal stem cells are CD34+ cells.26. The cell separation method according to claim 23 , wherein the nonwoven fabric comprises at least one selected from the group consisting of polyolefins claim 23 , polyamides and polyesters.27. The separation method according to claim 23 , wherein the separation material substantially does ...

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Номер записи: 58

02-01-2020 дата публикации

SYSTEM, APPARATUS AND METHOD FOR MATERIAL PREPARATION AND/OR HANDLING

Номер: US20200002663A1

Автор: Doebler Robert, Erwin Barbara, Hickerson Anna, IRVINE Bruce, Nadim Ali, Sterling James D., Talbot Ryan P., Woyski Denice

Принадлежит:

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency. 1. (canceled)2. A lysing apparatus , comprising:a container having at least one chamber to hold a material to be lysed and a lysing particulate material, the chamber having a first opening that provides fluid communication into the chamber from an exterior thereof;an impeller having a number of blades positionable in the chamber of the container; anda micromotor coupled to turn the impeller, at least a portion of the micromotor removably receivable in the first opening of the container to seal the first opening in use.3. The lysing apparatus of wherein the container comprises a second opening positioned above the first opening claim 2 , the second opening provides fluid communication into the chamber from the exterior thereof.4. The lysing apparatus of wherein the container comprises a third opening that provides fluid communication into the chamber from the exterior thereof.5. The lysing apparatus of wherein the first opening is the only opening in the container.6. The lysing apparatus of wherein the micromotor is disposable.7. The lysing apparatus of wherein the micromotor pulsates.8. The lysing apparatus of wherein the micromotor drives the impeller at a rate of greater than 10 claim 2 ,000 RPM.9. The lysing apparatus of wherein the ...

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Номер записи: 59

02-01-2020 дата публикации

Method for separating megakaryocytes and platelets and instrument for separating megakaryocytes and platelets

Номер: US20200002675A1

Автор: Tadanori Yamada, Toshiki Takei

Принадлежит: Fujifilm Corp

An object of the present invention is to provide a method for efficiently separating megakaryocytes and platelets produced from the megakaryocytes, and an instrument for efficiently separating megakaryocytes and platelets produced from the megakaryocytes. According to the present invention, a method for separating megakaryocytes and platelets, including a contact step of bringing a culture solution that contains at least megakaryocytes into contact with a substrate coated with a biocompatible polymer that adheres to the megakaryocytes via at least one of a VLA-4 integrin or a VLA-5 integrin; a culture step of culturing the megakaryocytes to produce platelets before and/or after the contact step; and a recovery step of recovering the culture solution after the contact step and the culture step is provided.

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Номер записи: 60

03-01-2019 дата публикации

IMPROVEMENTS IN AND RELATING TO CELL HARVESTING APPARATUS

Номер: US20190002817A1

Автор: Dowling Jim, Harris Dan

Принадлежит: GE HEALTHCARE BIO-SCIENCES CORP.

Disclosed herein is a cell harvesting instrument suitable for concentrating cells from a source suspension of cells and/or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump, at least one valve; and a processing kit removably insertable into the housing, said kit including a generally flat frame having or supporting plural sealed fluid paths arranged in a generally flat plane and such that fluids in the paths do not contact said mechanical elements, wherein at least portions of the fluid paths comprise flexible tubes, the outer surfaces of which are manipulateable by the or each fluid pump, to provide fluid flow in one or more of the paths and/or by the or each valve to restrict fluid flow in one or more of the paths. In an embodiment, the kit comprises also a fluid processing reservoir and a filter suitable for separating cells from fluid in said paths. A transfer mechanism for moving and weighing the fluid processing reservoir is disclosed also. 1. A pinch valve mechanism operable to pinch a flexible tube against a reaction surface , the mechanism comprisingat least one pivotable arm including on one side a finger to perform said pinching and on an opposing side a cam profile, anda linearly moveable actuator in use acting on the or each cam profile to move an associated finger.2. The mechanism of claim 1 , wherein the at least one pivotable arm comprises a first arm and a second arm each operable to pinch a different tube claim 1 , and wherein the first and second arms share the same actuator.3. The mechanism of claim 2 , wherein the cam profiles of the first and second arms are the same or similar in profile but opposed in orientation such that when the finger of the first arm provides a pinching action the finger of the second arm provides no pinching action claim 2 , and vice versa.4. The mechanism of claim 3 , wherein the opposed cam profiles of the first and second arms have a ...

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Номер записи: 61

03-01-2019 дата публикации

Device and Method for Isolation of Corneal Endothelial Cells

Номер: US20190002818A1

Автор: EATON, HAGGINS, Jr. Erik, JR. Robert, MURRAY Zachary, Varney Timothy

Принадлежит: The United States of America as Represented by the Secretary of the Army

An apparatus () for isolating corneal endothelial cells () (CECs) includes a base portion () having an interior recessed opening () with a bottom surface (). A convex projection () is centrally located on the bottom surface () and is configured to receive an inverted cornea (). A top portion () is configured to mate with the base portion (). The top portion () includes a fluid chamber () with a lower surface (). The lower surface () has an opening () therein in which the convex projection () projects when the top portion () is mated with the base portion (). 1. An apparatus for isolating corneal endothelial cells (CECs) , comprising:a base portion having an interior recessed opening with a bottom surface;a convex projection centrally located on the bottom surface and configured to receive an inverted cornea; anda top portion configured to mate with the base portion, the top portion including a fluid chamber with a lower surface, the lower surface having a central opening therein in which the convex projection projects when the top portion is mated with the base portion;wherein the lower surface of the top portion extends from and angles up and away from the opening therein and joins a side wall of the top portion, and the inverted cornea forms a fluid seal between the fluid chamber of the top portion and the interior recessed opening of the base portion and further wherein only an endothelial surface of the inverted cornea is exposed to the fluid chamber.2. The apparatus of claim 1 , wherein an angle between the lower surface of the top portion and a horizontal is in a range of 30 degrees to 70 degrees.3. The apparatus of claim 2 , wherein the angle is in a range of about 40 degrees to about 60 degrees.4. The apparatus of claim 1 , further comprising a groove formed on a lower exterior circumferential surface of the top portion and an O-ring disposed in the groove claim 1 , wherein the O-ring provides a friction fit between the top portion and the base portion.5. ...

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Номер записи: 62

07-01-2021 дата публикации

SYSTEM AND METHOD FOR RETRIEVING AND ANALYZING PARTICLES

Номер: US20210003496A1

Автор: Boniface Brian, Chow Will, Ganesan Karthik, Gleason Kyle, Gogoi Priyadarshini, Handique Kalyan, Payne Austin, Sharma Vishal

Принадлежит:

A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode. 1. A system for material processing at a capture substrate comprising a set of wells distributed across a broad face of the capture substrate , the system comprising: a tip, a terminal opening, and a void defined between the tip and the terminal opening, and', 'a positioning device coupled to the tip and providing a range of motion for positioning the terminal opening of the tip with respect to one or more wells of the set of wells of the capture substrate;, 'an aspiration and delivery subsystem comprising an illumination source, an optical sensor, an optical pathway from the illumination source to the capture substrate and from the capture substrate to the optical sensor; and', 'a processor in communication with the aspiration and delivery subsystem and the imaging subsystem;, 'an imaging subsystem comprising the imaging subsystem to generate an image dataset from the set of wells of the capture substrate, wherein the image dataset is captured from a direction perpendicular to the broad surface of the substrate,', 'the processor to generate an analysis characterizing a set of states of the set of wells and contents of the set of wells, and', 'the aspiration and delivery subsystem to, responsive to the analysis, perform at least one of an aspiration operation and a delivery operation with transmission of the tip to individual wells of the set of wells., 'wherein the processor comprises a non-transitory ...

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Номер записи: 63

03-01-2019 дата публикации

FILTRATION DEVICE WITH REMOVABLE PROTECTIVE MEMBER

Номер: US20190003935A1

Автор: BENNING Christopher A., CLARK Joshua, CURRAN SEAN, HEIGHTON SARAH JEAN, KENWOOD SHANNON SMITH, STORBECK Gene T.

Принадлежит: BOSTON SCIENTIFIC SCIMED, INC.

A cell filtration assembly adapted to capture cells from a biological sample during centrifugation includes a filter support member including a sample well and a filter membrane that spans the sample well. In some cases, the filter support member and/or the filter membrane may be adapted to be sectioned. In some cases, a protective element may be disposed over at least a portion of the filter support member in order to protect the filter support member from tissue processing reagents during processing of the cells captured from the biological sample. 1. A cell filtration assembly comprising:a filter support member including a sample well;a filter membrane extending across the sample well;the filter support member and/or the filter membrane configured to be sectioned; anda protective element disposed over at least a portion of the filter support member, the protective element configured to protect the filter support member from tissue processing reagents during processing of the cells captured from the biological sample.2. The cell filtration assembly of claim 1 , wherein the protective element is adapted to be removed prior to sectioning.3. The cell filtration assembly of claim 2 , wherein the protective element comprises a removable shell.4. The cell filtration assembly of claim 1 , wherein the protective element is adapted to be sectioned while disposed over at least a portion of the filter support member.5. The cell filtration assembly of claim 4 , wherein the protective element comprises a polymeric coating.6. The cell filtration assembly of claim 4 , wherein the protective element comprises Parylene.7. The cell filtration assembly of claim 1 , wherein the filter support member comprises a material that is at least partially miscible in xylene.8. The cell filtration assembly of claim 1 , wherein the filter support member comprises a wax.9. The cell filtration assembly of claim 1 , wherein the filter support member comprises paraffin.10. A cell filtration ...

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Номер записи: 64

20-01-2022 дата публикации

PROCESS AND APPARATUS FOR PRODUCING EXOSOMES

Номер: US20220017852A1

Автор: Barile Lucio, Favalli Alessandro, Nicolis Daniele, Robortella Roberto, Stefanini Igor Sergio Laerte, Tavilla Agatino Christian

Принадлежит:

Process and apparatus for producing exosomes operating continuously, in which a product containing exosomes is extracted from an incubator (); said product is subjected to tangential filtering in a concentrator (); the product is fed in two separate flows () to two sides () of the concentrator () separated by a semi-permeable wall obtaining a concentration of the exosomes in one of the two flows; a flow of product enriched with exosomes () is extracted from the concentrator; a diluted product () after transit through the concentrator is recirculated to the incubator; thermal conditioning (), gaseous conditioning () and in-line pH control are provided. 1. Continuous process for the production of exosomes , comprising:a) cell culture with release of exosomes carried out in an incubator obtaining from said incubator a product containing exosomes and an aqueous culture medium;b) continuously feeding a first portion of said product to a first side of a concentrator device;c) continuously feeding a second portion of said product to a second side of said concentrator device;d) wherein said first side and said second side of the concentrator device are in communication with each other via a semi-permeable wall which is permeable to the aqueous culture medium and substantially impermeable to the exosomes;e) wherein the transit of a first flow of said product and a second flow of said product respectively through the first side and the second side of the concentrator device causes a passage of the aqueous culture medium from the second side towards the first side, across said wall, and a consequent increase in the concentration of exosomes in the product which transits through the second side;f) collecting a product enriched with exosomes from said second side of the concentrator device; andg) collecting a diluted product from said first side of the concentrator device and recirculating said diluted product to said incubator,wherein the aforesaid process steps a)-g) are ...

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Номер записи: 65

20-01-2022 дата публикации

SYSTEMS, DEVICES AND METHODS FOR IDENTIFICATION, SELECTIVE ABLATION, AND SELECTION AND COLLECTION OF SINGLE CELLS

Номер: US20220017858A1

Автор: MACKAY Sean, ZHENG Rui

Принадлежит:

Embodiments of the present disclosure are directed to systems, devices, and methods for the selective collection of cells from a heterogeneous cell population, including highly multiplexed detection of secreted and intracellular macromolecules and the targeted laser-assisted ablation of cells identified to be positive or negative for a given biomarker or phenotype. The resulting non-ablated cells can be collected individually or pooled to form a homogenous cell population for further processing including safe and efficacious cellular therapies. 1. A selective cell collection and/or sorting method for at least one of selectively collecting and sorting cells comprising: the substrate including a first surface that is releasably coupled to a transparent cover having a second surface, forming an assembly,', 'the second surface having a plurality of capture agents, and', 'the volume of fluid is in fluid communication with the second surface;, 'loading or otherwise placing into each of a plurality of isolated chambers of a substrate, a cell and a volume of fluid, wherein the production of one or more cellular components by each cell, and', 'the one or more cellular components configured to bind with at least one of the capture agents of the surface so as to form at least one capture agent cellular component complex;, 'maintaining each cell under one or more conditions sufficient to permitfor each of the cells, detecting the at least one capture agent cellular component complex;identifying at least one cell for at least one of collection and removal; andcollecting the at least one cell.2. The method of claim 1 , further comprising ablating the cell identified for removal.3. The method of claim 2 , wherein ablating comprises contacting a respective isolated chamber comprising the cell for removal with a laser.4. The method of claim 3 , wherein the laser is configured to lyse the cell.5. The method of any of - claim 3 , wherein a plurality of cells comprises a heterogeneous ...

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Номер записи: 66

12-01-2017 дата публикации

NON-PLANAR AND NON-SYMMETRICAL PIEZOELECTRIC CRYSTALS AND REFLECTORS

Номер: US20170008029A1

Автор: Chitale Kedar, Dutra Brian, Gilmanshin Rudolf, III Thomas J, JR. Walter M, Kennedy, Lipkens Bart, Mealey Dane, Presz, Sokolowski David

Принадлежит:

An acoustophoretic device is disclosed. The acoustophoretic device includes an acoustic chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer includes a piezoelectric material driven by a voltage signal to create a multi-dimensional acoustic standing wave in the acoustic chamber emanating from a non-planar face of the piezoelectric material. A method for separating a second fluid or a particulate from a host fluid is also disclosed. The method includes flowing the mixture through an acoustophoretic device. A voltage signal is sent to drive the ultrasonic transducer to create the multi-dimensional acoustic standing wave in the acoustic chamber such that the second fluid or particulate is continuously trapped in the standing wave, and then agglomerates, aggregates, clumps, or coalesces together, and subsequently rises or settles out of the host fluid due to buoyancy or gravity forces, and exits the acoustic chamber. 1. An acoustophoretic device , comprising:an acoustic chamber having at least one inlet and at least one outlet;at least one ultrasonic transducer located on a wall of the acoustic chamber, the at least one ultrasonic transducer including a piezoelectric material driven by a voltage signal to create a multi-dimensional acoustic standing wave in the acoustic chamber emanating from a non-planar face of the piezoelectric material; anda reflector located on a wall on the opposite side of the acoustic chamber from the at least one ultrasonic transducer.2. The acoustophoretic device of claim 1 , wherein the non-planar face of the piezoelectric material is poled in a direction substantially perpendicular to a second face of the piezoelectric material.3. The acoustophoretic device of claim 1 , wherein the non-planar face of the piezoelectric material is defined by a step function or a smooth function.4. The acoustophoretic device of claim 1 , wherein the reflector has a non-planar surface.5. The acoustophoretic device of claim 4 , wherein ...

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Номер записи: 67

14-01-2021 дата публикации

Magnetic mixing apparatus

Номер: US20210008513A1

Автор: David Rolfe, Thomas H. Cauley, III

Принадлежит: Talis Biomedical Corp

This disclosure relates to a magnetic mixing apparatus that mixes a sample contained in a mixing chamber using a stir bar, while minimizing the amount of contact between the stir bar and walls of the mixing chamber. In one aspect, the apparatus comprises a ferromagnetic stir bar contained in the mixing chamber, and a driving magnet and a driven magnet located on opposite sides of the mixing chamber. The driving magnet, the driven magnet, and the ferromagnetic stir bar are each capable of rotating about a respective axis. The driving magnet, the driven magnet, and the ferromagnetic stir bar are magnetically coupled such that rotation of the driving magnet induces rotation of the driven magnet and rotation of the driving magnet and the driven magnet induce rotation of the ferromagnetic stir bar. In some embodiments, rotation of the ferromagnetic stir bar within the mixing chamber mixes the sample contained within the mixing chamber.

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Номер записи: 68

10-01-2019 дата публикации

METHODS FOR ENHANCED PRODUCTION AND ISOLATION OF CELL-DERIVED VESICLES

Номер: US20190008902A1

Автор: ANDERSON Johnathon D., Bauer Gerhard, FURY Brian, NOLTA Jan A.

Принадлежит:

This disclosure relates to populations and compositions of purified cell-derived vesicles and uses thereof. One aspect of the disclosure relates to methods for purifying the cell-derived vesicles. 1. A highly purified population of cell-derived vesicles prepared by culturing stem cells producing the cell-derived vesicles under conditions of hypoxia and low serum conditions , optionally wherein the cell-derived vesicles comprise exosomes and/or microvesicles.2. (canceled)3. The purified population of claim 1 , wherein the cell-derived vesicles are isolated from one or more stem cells of the group of adult stem cells claim 1 , embryonic stem cells claim 1 , embryonic-like stem cells claim 1 , neural stem cells claim 1 , mesenchymal stem cells claim 1 , or induced pluripotent stem cells.4. (canceled)5. The purified population of claim 1 , wherein the cell-derived vesicles of the population further comprise at least one exogenous nucleic acid and/or at least one exogenous protein claim 1 , optionally wherein the population of cell-derived vesicles do not comprise exogenous VEGFR and/or VEGF.6. The purified population of claim 5 , wherein the exogenous nucleic acid encodes a micro RNA (miRNA) claim 5 , optionally wherein the miRNA is selected from the group consisting of miR-150 claim 5 , miR-126 claim 5 , miR-132 claim 5 , miR-296 claim 5 , and let-7.7. (canceled)8. The purified population of claim 5 , wherein the exogenous protein is one or more of platelet derived growth factor receptor (PDGFR) claim 5 , Collagen claim 5 , Type 1 claim 5 , Alpha 2 (COL1A2) claim 5 , Collagen claim 5 , Type VI claim 5 , Alpha 3 (COL6A3) claim 5 , EGF-like repeats- and discoidin i-like domains-containing protein 3 (EDIL3) claim 5 , epidermal growth factor receptor (EGFR) claim 5 , fibroblast growth factor receptor (FGFR) claim 5 , fibronectin (FN1) claim 5 , Milk fat globule-EGF factor 8 (MFGE8) claim 5 , lectin claim 5 , galactoside-binding claim 5 , soluble claim 5 , 3 binding protein ...

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Номер записи: 69

09-01-2020 дата публикации

SYSTEM AND METHOD FOR SELECTING AND CULTURING CELLS

Номер: US20200009309A1

Автор: Binninger Steven, Hamilton Melanie, Kadakia Avnie A., Olson Bret M., Schlinker Alaina, THOMPSON Kyle, Wegener Christopher J., Wolok Kyle

Принадлежит:

A cell processing system includes at least one processor connectable to a source container filled with a biological fluid, the processor including a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet, first and second containers selectively connected to the first outlet; and a magnet. The system also includes a controller configured to operate the spinning membrane to receive biological fluid and to direct the target cells to the first container, to pause to permit magnetic particles to be associated with the target cells in the first container, to operate the spinning membrane to receive the contents of the first container with the magnet applied to the target cells associated with the magnetic particles, to remove or deactivate the magnet, and to transfer the target cells to the second container. 1. A cell processing system comprising: a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet;', 'at least a first container and a second container selectively connected to the first outlet; and', 'a magnet; and, 'at least one processor connectable to a source container filled with a biological fluid, the at least one processor comprising to operate the spinning membrane to receive biological fluid from the source container and to direct the target cells to the first container;', 'to pause operation of the processor to permit magnetic particles to be associated with the target cells in the first container;', 'to operate the spinning membrane to receive the contents of the first container with the magnet applied to the target cells associated with the magnetic particles in the first container;', 'to remove and/or deactivate the magnet applied to the target cells associated with the magnetic particles in the first container;', 'to transfer the target cells to the second container after removal and/or ...

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Номер записи: 70

14-01-2016 дата публикации

FILTRATION SYSTEM AND USE THEREOF

Номер: US20160010052A1

Автор: Fuchs Al, Nutter Dana M., Preidel Thomas, Stimpson Eric, Wainwright Norman R.

Принадлежит:

The invention relates to a filtration system for use in a method of determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using and manufacturing such a filtration system. The filtration system includes a cup with an upper portion and a ring portion, where the ring portion is separably coupled to the upper portion. 1. A cell capture system for receiving a fluid sample , the system comprising: (i) an upper portion,', '(ii) a ring having a periphery, wherein the upper portion is separably coupled to the ring, and', '(iii) a fluid permeable membrane attached to the periphery to produce a fluidic seal between the membrane and the ring, wherein a portion of the membrane is adapted to retain cells thereon; and, '(a) a cup comprising(b) a base configured to receive the ring.2. The system of claim 1 , wherein the membrane portion (i) defines a plurality of pores having an average diameter less than about 1 μm so as to permit fluid to traverse the second portion of the membrane while retaining cells thereon and (ii) is substantially non-autofluorescent when exposed to light having a wavelength in a range from about 350 nm to about 1000 nm.3. The system of claim 1 , wherein the membrane portion has a flatness tolerance of up to about 100 μm.4. The system of claim 1 , wherein the cup is adapted to direct a fluid claim 1 , when introduced into the upper portion claim 1 , toward the membrane portion.5. The system of claim 1 , wherein the ring is integrally formed with the upper portion.6. The system of claim 5 , wherein the ring is separably coupled to the upper portion with a frangible connection.7. The system of claim 6 , wherein the frangible connection comprises a thin wall at an intersection of the upper portion and the ring.8. The system of claim 7 , wherein the thin wall intersection defines a circumferential groove.9. The system of claim 6 , wherein the frangible connection defines a circumferential groove.10. ...

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Номер записи: 71

09-01-2020 дата публикации

SYSTEM AND METHOD FOR BUOYANT PARTICLE PROCESSING

Номер: US20200009614A1

Автор: Hermanson Greg, Hyun Bill, Kelchner Vanessa, McNaughton Brandon H., Petlakh Nadia, Younger John G.

Принадлежит:

A system for buoyant particle processing includes: a reaction vessel, a stirring mechanism, a set of one or more pumps, and a filter. The system can additionally or alternatively include a set of pathways and/or any other suitable component(s). A method for buoyant particle processing includes: stirring the contents of a reaction vessel; washing a set of buoyant particles; and filtering the contents of the reaction vessel. Additionally or alternatively, the method can include any or all of: preprocessing the set of buoyant particles; adding a set of inputs to the reaction vessel; washing the set of buoyant particles; repeating one or more; and/or any other suitable process(es). 1. A system for processing a set of buoyant particles , the system comprising: a first inlet configured to receive a wash buffer;', 'a second inlet configured to receive a set of processing materials;', 'a third inlet configured to receive the set of buoyant particles; and', 'a first outlet;, 'a reaction vessel, the reaction vessel comprisinga stirring subsystem comprising a stir rod and an impeller, the impeller arranged within the reaction vessel; a set of hollow fibers, each of the set of hollow fibers having an inner diameter larger than a diameter of the set of buoyant particles;', 'a fourth inlet configured to receive the set of buoyant particles;', 'a second outlet;, 'a tangential flow filter comprisinga pump arranged downstream of the first outlet, wherein the pump comprises a diaphragm pump; and the first inlet with a wash buffer container, the wash buffer container comprising the wash buffer;', 'the second inlet with a processing material container, the processing material container comprising the set of processing materials;', 'the third inlet with the second outlet; and', 'the first outlet with the fourth inlet., 'a set of fluidic pathways configured to fluidly connect2. The system of claim 1 , wherein each of the set of buoyant particles has a particle diameter between 10 and 30 ...

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Номер записи: 72

11-01-2018 дата публикации

ACOUSTIC PERFUSION DEVICES

Номер: US20180010085A1

Автор: Chitale Kedar, III Thomas J., Kennedy, Lipkens Bart, Miller Erik, Presz Walter M., Ross-Johnsrud Benjamin, Winiarski Lauren

Принадлежит:

Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn. 1. An acoustic perfusion device , comprising:an acoustic chamber;at least one ultrasonic transducer coupled to the acoustic chamber;at least one reflector opposite the at least one ultrasonic transducer across the acoustic chamber;the at least one ultrasonic transducer configured to be excited to generate an acoustic standing wave with the at least one reflector;a flow path arranged in the acoustic chamber to bring a fluid and particle mixture proximate to the acoustic standing wave, such that a perfusate turbidity is reduced by about 50% or greater.2. The device of claim 1 , wherein the flow path is shaped to generate a tangential flow path proximate to the acoustic standing wave.3. The device of claim 1 , wherein a pressure rise and an acoustic radiation force on cells are generated at an upstream interface region of the acoustic standing wave to clarify fluid passing through the acoustic standing wave.4. The device of claim 1 , wherein the at least one reflector is made of a transparent material.5. The device of claim 1 , wherein the at least one ultrasonic transducer further comprises two or more ultrasonic transducers.6. The device of claim 5 , where the at least one reflector is located between the two or more ultrasonic transducers.7. The device of claim 1 , wherein the acoustic standing wave generates an acoustic radiation force with an axial force component and a lateral force component that are of the same order of magnitude.8. ...

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Номер записи: 73

11-01-2018 дата публикации

NOVEL SYRINGE SYSTEM FOR FLUID SEPARATION

Номер: US20180010086A1

Автор: Cho Sohyung, Gupta Ashim, Neumeister Michael W.

Принадлежит: Board of Trustees of Southern Illinois University

A syringe device for separating liquids of different densities is provided with a hollow syringe barrel, a perforated plunger seal with a seal hole, and a hollow plunging tube with a closed bottom with at least one tube hole. The perforated plunger seal has an outer perimeter that resides flush against an interior surface of the hollow syringe barrel. The tube hole is in operational relationship with the seal hole. Optionally, a relief hole is provided on a top portion of the hollow plunging tube to allow a user to create vacuum pressure as necessary. 1) A syringe device for separating liquids of different densities comprising of:a hollow syringe barrel,a perforated plunger seal with an outer perimeter that resides flush against an interior surface of said hollow syringe barrel with an at least one seal hole in said perforated plunger seal,a hollow plunging tube with a relief hole located at an upper portion of said hollow plunging tube, where said hollow plunging tube is threadably coupled to said perforated plunger seal via a bottom portion of a wall of said hollow plunging tube that resides above and is in operational relationship to said at least one seal hole in said perforated plunger seal,an at least one female interlocking thread on said perforated plunger seal is aligned to correspond with an at least one male interlocking thread located adjacent to the bottom portion of said hollow plunging tube, andsaid at least one seal hole in said perforated plunger seal resides to communicate a hollow plunging tube cavity of said hollow plunging tube with a hollow syringe barrel cavity located within said hollow syringe barrel when said hollow plunging tube is in an open position.2) The syringe device of further comprising of:a beveled needle hub at a lower end of said hollow syringe barrel to allow fluids to be pushed out of said hollow syringe barrel cavity of said hollow syringe barrel.3) The syringe device of wherein:said hollow plunging tube is rotated to set ...

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Номер записи: 74

11-01-2018 дата публикации

PREPARING ANTIGEN-SPECIFIC T CELLS USING A SELF-ENCLOSED PROCESSING SYSTEM THAT CONTAINS BOTH A CENTRIFUGE AND A MAGNETIC SEPARATION COLUMN

Номер: US20180010087A1

Автор: Biehl Martin, Kabaha Eiad, Lantow Holger, Miltenyi Stefan, Neuschaefer Niklas Elmar, Schimmelpfennig Winfried, Schulz Juergen

Принадлежит: Miltenyi Biotec GmbH

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (), a pump () and a plurality of valves (-) configured to at least partially control fluid flow through a fluid circuitry and a separation column () positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column. 1. A method of enriching a defined population of target cells from a mixed population of blood cells using an apparatus that is configured for processing such cells using a sterile tubing set;wherein the tubing set comprises:(a) a first connecting member configured to receive and deliver the sample to a rotatable container,(b) said rotatable container, which is configured to rotate about an axis and thereby to apply centrifugal force to a sample contained in a processing chamber in the container so as to separate cells from the sample from other components of the sample;(c) a second connecting member configured to transfer cells processed by the rotatable container to a cell separation column;(d) said separation column, which is configured to separate labeled cells from unlabeled cells; and(e) a third connecting member configured to transfer cells separated by the cell separation column to a product collection container;wherein the method comprises:(1) delivering the sample through the first connecting member into the tubing set; and(2) operating the apparatus such that the sample is ...

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Номер записи: 75

14-01-2021 дата публикации

METHOD FOR LARGE-SCALE PREPARATION OF PURIFIED PREPARATION OF RECOMBINANT LENTIVIRAL VECTOR AT GMP GRADE

Номер: US20210009966A1

Автор: Hong Yi, WANG FEI, Yan Ting, Ying Jiangguo, Zhang Haojie, Zhang Li, Zhang Luyi, Zhao Dijun

Принадлежит:

Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors. 2. The method of claim 1 , wherein the anion resin is selected from the group consisting of Capto Q claim 1 , Capto ImpRes claim 1 , and Capto DEAE.3. The method of claim 1 , wherein the chromatography purification is to perform anion chromatography for crude purification firstly claim 1 , and then perform multi-mode composite chromatography for elaborate purification.4. The method of claim 1 , wherein in step (d) claim 1 , the purified recombinant lentiviral vectors have one or more features selected from the following group:{'sup': 6', '9, '(p1) the biological titer of recombinant lentiviral vectors is 1.0×10Tu/mL;'}(p2) the residue of BSA is <50 ng/mL;(p3) the content of endotoxin is <1 EU/mL.5. The method of claim 1 , wherein in step (c) claim 1 , the concentration is performed by ultrafiltration.6. A purified recombinant lentivirus prepared by the method of .7. (canceled)8. A purification device for performing the method of claim 1 , which comprises:(S1) an optional first container, wherein the first container is used for holding raw material of recombinant lentivirus to be purified;(S2) a microfiltration unit, wherein the microfiltration unit is used to perform microfiltration on the ...

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Номер записи: 76

10-01-2019 дата публикации

ARRAY FOR PROCESSING MATERIALS

Номер: US20190010662A1

Автор: Masterman Thomas Craig, Medoff Marshall, PARADIS Robert

Принадлежит:

Materials (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems equipment, and methods are described that can be used to treat feedstock materials, such as cellulosic and/or lignocellulosic materials, using an array of vaults. 1. A treatment operating unit , comprising:a plurality of enclosure systems, each enclosure system including one or more vaults, andwithin each vault, an irradiation device and a treatment conveyor.2. The operating unit of claim 1 , wherein the enclosure systems are arranged in rows.3. The operating unit of claim 2 , wherein the rows extend in a first direction claim 2 , and wherein each enclosure system comprises two or more vaults extending in a direction generally perpendicular to the first direction.4. The operating unit of claim 3 , wherein the first and second vaults of each enclosure share a common wall.5. The operating unit of claim 4 , wherein each first vault is configured to accept untreated biomass from a storage facility claim 4 , and wherein the biomass material is treated in each vault utilizing the irradiation device and the treatment conveyor.6. The operating unit of claim 5 , wherein the first vault of each enclosure system further encloses equipment configured to transfer treated biomass from the first vault to the second vault of the enclosure system.7. The operating unit of claim 1 , wherein the irradiation device comprises an electron accelerator.8. The operating unit of claim 1 , wherein the treatment conveyor comprises a vibratory conveyor.9. A method for producing treated materials claim 1 , the method comprising;partitioning a material into a plurality of material portions,conveying the material portions into a plurality of first vaults, each first vault accepting one of the material portions,treating the material portions in the vaults,conveying the material portions out of the ...

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Номер записи: 77

09-01-2020 дата публикации

ARRAYED LYSER AND HOMOGENIZER SYSTEMS WITH MULTIPLE AGITATOR DEVICES

Номер: US20200010789A1

Автор: Brown Mark, Doebler Robert W., Ferguson Tanya, IRVINE Bruce, Sterling James D.

Принадлежит:

Systems and methods for the efficient agitation of tissue samples. A device may include a plurality of chambers that each receives samples therein. The plurality of chambers may be uniformly spaced with respect to a least one dimension, to form a one dimensional or two dimensional array. Each of the chambers may include an opening and an agitator device in fluid contact with the sample disposed within the chamber. The agitator devices may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The system may include an electrical energy source electrically coupled to the plurality of micromotors to rotate the impellers sufficient to agitate the sample as required for a particular activity (e.g., homogenization, lysis). 1. A system for homogenization and lysis of biological samples , the system comprising:a plurality of chambers spaced apart from each other in an array along at least a first dimension, each of the plurality of chambers sized and dimensioned to receive fluid and a biological sample therein; anda plurality of agitator devices each of which correspond to one of the plurality of chambers, at least a portion of each of the plurality of agitator devices positionable within the corresponding one of the plurality of chambers, and in operation each of the plurality of agitator devices selectively agitates the fluid and biological sample disposed in the corresponding one of the plurality of chambers.2. The system of wherein the plurality of chambers are uniformly spaced apart from each other in a second dimension orthogonal to the first dimension.3. The system of wherein the plurality of chambers are uniformly spaced apart from each other in the first dimension by a first distance which extends between the center of adjacent chambers along the first dimension claim 2 , and the plurality of chambers are ...

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Номер записи: 78

18-01-2018 дата публикации

LARGE SCALE ACOUSTIC SEPARATION DEVICE

Номер: US20180015392A1

Автор: Barnes Jason, Chitale Kedar, JR. Walter M., King Jeffrey, Lipkens Bart, McCarthy Brian, Mealey Dane, Presz, Ross-Johnsrud Ben

Принадлежит:

Devices for separating a host fluid from a second fluid or particulate are disclosed. The devices include an acoustic chamber, a fluid outlet at a top end of the acoustic chamber, a concentrate outlet at a bottom end of the acoustic chamber, and an inlet on a first side end of the acoustic chamber. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave in the acoustic chamber that traps and separates particulates (e.g. cells) from a host fluid. The host fluid is collected via the fluid outlet, and the particulates are collected via the concentrate outlet. The device is a large-scale device that is able to process liters/hour, and has a large interior volume. 1. A method for separating a secondary fluid or particulate from a mixture with a host fluid , comprising: an acoustic chamber;', 'at least one ultrasonic transducer coupled to the acoustic chamber and configured to generate an acoustic standing wave in the acoustic chamber;', 'a reflector across the acoustic chamber from the at least one ultrasonic transducer; and', 'an interior volume of at least 40 cubic inches;, 'placing the mixture in an acoustophoretic device that comprisesexciting the at least one transducer to generate the acoustic standing wave in the acoustic chamber; andtrapping the secondary fluid or particulate via the acoustic standing wave such that the secondary fluid or particulate agglomerates, aggregates, clusters, or coalesces together and separates from the host fluid.2. The method of claim 1 , further comprising flowing the mixture through the acoustophoretic device.3. The method of claim 2 , further comprising flowing the mixture through a dump diffuser into the acoustic chamber.4. The method of claim 2 , further comprising flowing the mixture in a flow direction normal to an axial direction of the acoustic standing wave generated by the at least one ultrasonic transducer.5. The method of claim 2 , further comprising flowing the mixture into the acoustic ...

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Номер записи: 79

19-01-2017 дата публикации

SPHEROID-PRODUCING DEVICE, METHOD FOR RECOVERING SPHEROIDS, AND METHOD FOR PRODUCING SPHEROIDS

Номер: US20170015966A1

Автор: SUMI Shoichiro

Принадлежит:

The present invention provides a device for producing a large number of uniform spheroids by an easy method. The spheroid-producing device () at least includes a first surface (), a second surface (), and a plurality of wall surfaces (). The second surface () faces the first surface (). The respective wall surfaces () constitute a plurality of holes penetrating through the first surface and the second surface. In addition, an equivalent diameter of inscribed circles of openings in the first surface () is greater than an equivalent diameter of inscribed circles of openings in the second surface (). 1: A spheroid-producing device comprising:a first surface;a second surface, the second surface being a back side surface of the first surface; anda plurality of wall surfaces configured to constitute a plurality of holes penetrating through the first surface and the second surface, wherein an equivalent diameter of inscribed circles of openings in the first surface is greater than an equivalent diameter of inscribed circles of openings in the second surface.2: The spheroid-producing device according to claim 1 , wherein the equivalent diameter of the inscribed circles of the openings in the second surface is within a range of 200 micrometers to 1 cm.3: The spheroid-producing device according to claim 1 , wherein at least portions of the respective wall surfaces include inclinations with an angle greater than one degree and smaller than 90 degrees with respect to the second surface.4: The spheroid-producing device according to claim 1 , whereina culture medium is poured into the respective holes from the first surface, {'br': None, 'i': X', '+pX=', 'L,, 'sup': '2', '(⅔)α2γ'}, 'when a contact angle θc between surfaces of a material forming the respective wall surfaces and the culture medium is within a range of −1 Подробнее

Номер записи: 80

03-02-2022 дата публикации

CELL SORTING DEVICE AND METHOD

Номер: US20220034785A1

Автор: Gu Yi, Lo Yu-Hwa

Принадлежит:

A cell sorting system is provided to comprise: an imaging device including abeam scanner scanning abeam along a first direction to obtain a cell image data including fluorescent information or cell image information of a cell, the beam applied to the cell flowing in a channel along a second direction with an angle to the first direction; a data processing and control device in communication with the imaging device, the data processing and control device including a processor configured to process the cell image data obtained by the imaging device to determine one or more properties associated with the cell from the processed cell image data and to produce a control command based on a comparison of the determined one or more properties with a sorting criteria, and a cell sorting device in communication with the imaging device and the data processing and control device. 1. A cell sorting system , comprising:an imaging device including a beam scanner scanning a beam along a first direction to obtain a cell image data including fluorescent information or cell image information of a cell, the beam applied to the cell flowing in a channel along a second direction with an angle to the first direction;a data processing and control device in communication with the imaging device, the data processing and control device including a processor configured to process the cell image data obtained by the imaging device to determine one or more properties associated with the cell from the processed cell image data and to produce a control command based on a comparison of the determined one or more properties with a sorting criteria, wherein the control command is produced during the cell flowing in the channel and is indicative of a sorting decision determined based on one or more cellular attributes ascertained from the cell image data; anda cell sorting device in communication with the imaging device and the data processing and control device, the cell sorting device including two ...

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Номер записи: 81

21-01-2016 дата публикации

SPERM SELECTION UNIT STRUCTURE, SPERM SCREENING DEVICE PROVIDED WITH SPERM SELECTION UNIT STRUCTURE, AND METHOD OF PREPARING SEMEN FOR FERTILIZATION

Номер: US20160017273A1

Автор: Maeda Hideaki, Miyazaki Masaya, NAGATA Maria Portia, Yamashita Kenichi

Принадлежит:

A sperm selection unit structure for efficiently separating sperm having favorable motility includes: a storage part which stores semen acquired from an animal therein; a buffer solution flow passage which communicates with the storage part and in which a buffer solution flows in the direction toward the storage part as a laminar flow; and a swim-up passage which communicates with the storage part such that the sperm swim up in the buffer solution flow passage from the storage part, wherein a downstream part of the swim-up passage is formed of a flow passage having a wide width where the buffer solution flows as a slow flow while maintaining a laminar flow state of the buffer solution, and a downstream end portion of the flow passage is opened in a state where the downstream end portion faces a wall surface of the storage part.

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Номер записи: 82

21-01-2016 дата публикации

DEVICE AND METHOD FOR TREATING A FILTRATION MEDIUM

Номер: US20160017274A1

Автор: Pflanz Karl, Scholz Alexandra

Принадлежит:

A device () and a method are provided for treating a porous filtration medium () having a receiving unit () with of a receiving part () and a base part (). The porous filtration medium () can be lifted by the receiving part () from a lower part () of a filtration device (), and the receiving part () with the porous filtration medium () can be mounted on the base part (). The receiving part () is latchable to the base part (). The base part (), towards the filtration medium () has an incubation chamber () connected to a base part () outlet () that faces away from the receiving part (), and the outlet () has a projection onto which a receiving vessel () containing a solvent () for dissolving the porous filtration medium () can be detachably pushed on. 113712563753332537656. A device () for treating a porous filtration medium () , the device () having a receiving unit () with a receiving part () and a base part () , the porous filtration medium () is receivable via the receiving part () by a lower part () of a filtration device () , and the receiving part () with the porous filtration medium () is mountable on the base part () , and the receiving part () is formed so as to be latchable to the base part () , wherein:{'b': 6', '37', '17', '3', '6', '5, 'the base part (), towards the filtration medium () has an incubation chamber () that is connected to an outlet () of the base part () that is facing away from the receiving part (), and'}{'b': 3', '24', '4', '28', '37, 'the outlet () has a projection () onto which a receiving vessel () containing a solvent () for dissolving the porous filtration medium () can be detachably pushed on.'}2. The device of claim 1 ,wherein{'b': 17', '6', '3, 'the incubation chamber () of the base part () is conical towards the outlet ().'}3. The device of claim 1 ,wherein{'b': 28', '37, 'the solvent () for dissolving the filtration medium () is an organic solvent.'}4. The device of claim 3 ,wherein{'b': '28', 'the solvent () is chloroform or ...

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Номер записи: 83

18-01-2018 дата публикации

METHOD FOR ISOLATING, REMOVING AND ANALYZING CELLS

Номер: US20180016542A1

Автор: FUKAMIZU Akiyoshi, Hagihara Masahiko, KIM Jun-Dal, WADA Yukinori

Принадлежит:

The purpose of the present invention is to provide a method for isolating, removing and analyzing cells. Provided is a method for isolating, removing and analyzing cells by filtering a liquid specimen containing cells through a porous polyimide film, and subjecting the cells captured by the film which did not pass through the porous polyimide film, or the cells in the liquid specimen which did pass through the porous polyimide film, to an examination of one or more cell properties selected from a group consisting of cell number or type, internal or external cell structure, type or amount of cell surface antigen, type or amount of material secreted from cells, cell adhesion, and cell survival rate. 1. A method for isolating , removing and/or analyzing cells , including: filtering a liquid sample containing cells with a polyimide porous film , and examining one or more cell properties selected from the group consisting of the number or type of cells , external or internal structure of the cells , type or amount of cell surface antigens , type or amount of substances secreted from the cells , cell adhesion and cell survival rate for cells that were captured in the polyimide porous film without being filtered by the film as well as cells in the liquid sample that passed through the polyimide porous film.2. The method according to claim 1 , wherein the target cells are activated cells.3. The method according to claim 2 , wherein the activated cells are activated leukocytes.4. The method according to any one of to claim 2 , which includes a step for pretreating all or a portion of the surface of the polyimide porous film prior to the filtration step.5. The method according to claim 4 , wherein surface treatment of the polyimide porous film is carried out using one or more agents or treatment methods selected from the group consisting of an anticoagulant claim 4 , collagen claim 4 , poly-L-lysine claim 4 , UV light claim 4 , plasma irradiation and PDS bound to a ...

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Номер записи: 84

18-01-2018 дата публикации

SYSTEM FOR GENERATING HIGH CONCENTRATION FACTORS FOR LOW CELL DENSITY SUSPENSIONS

Номер: US20180016570A1

Автор: Dionne Jason, GHOSHAL Goutam, Lipkens Bart

Принадлежит:

Acoustophoretic devices and methods for concentrating targeted biological cells in a reduced volume using multi-dimensional acoustic standing waves are disclosed. The methods include flowing a mixture of a host fluid and the biological cells through an acoustophoretic device. The acoustophoretic devices include an inlet, an outlet, and a flow chamber having an ultrasonic transducer-reflector pair. Biological cells, such as T cells, are separated from a host fluid for utilization in allergenic or autologous cell therapies. The disclosed devices and methods are capable of concentrating biological cells to at least 100 times their original cell concentration. The disclosed methods and devices are further capable of decreasing an original feed volume to a final concentrated volume that is less than one percent of the original feed volume. 1. A method for concentrating target particles in a host fluid , comprising: an acoustic chamber;', 'at least one ultrasonic transducer coupled to the acoustic chamber, the at least one ultrasonic transducer configured to generate an acoustic standing wave in the acoustic chamber; and', 'a reflector on an opposite side of the acoustic chamber from the at least one ultrasonic transducer;, 'placing an original concentration mixture of the host fluid and the target particles in an acoustophoretic device, the acoustophoretic device comprisingexciting the at least one ultrasonic transducer to generate the acoustic standing wave in the acoustic chamber; andconcentrating the target particles via the acoustic standing wave by at least an order of magnitude difference from the original concentration mixture.2. The method of claim 1 , further comprising concentrating the target particles to a final concentration of about 150 times to about 300 times the original concentration mixture.3. The method of claim 1 , further comprising concentrating the target particles to a final concentration of about 300 times to about 600 times the original ...

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Номер записи: 85

18-01-2018 дата публикации

RECONFIGURABLE PROCESSING ENCLOSURES

Номер: US20180016745A1

Автор: Masterman Thomas Craig, Medoff Marshall, PARADIS Robert

Принадлежит:

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or other materials are processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems and methods are described that can be used to treat feedstock materials, such as cellulosic and/or lignocellulosic materials, in a vault in which the walls and optionally the ceiling include discrete units. Such vaults are re-configurable. 1. A treatment facility comprising:a vault, having walls, ceiling, and a foundation; andwithin the vault, a material conveying system configured to convey biomass under an electron beam, wherein each of the walls comprises a plurality of discrete reconfigurable units and at least one unit of the plurality of units comprises a high Z material.2. The facility as in claim 1 , wherein the ceiling comprises a plurality of discrete units.3. The facility as in claim 1 , wherein the high Z material is a metal with a Z value above 25.4. The facility as in claim 1 , further comprising an electron irradiation device supported by the ceiling of the vault and disposed to irradiate biomass conveyed by the conveying system.5. The facility as in claim 4 , wherein the irradiation device weighs at least 5 Tons.6. The facility as in claim 4 , wherein the irradiation device weighs at least 10 tons.7. The facility as in claim 4 , wherein the irradiation device weighs between about 5 and about 20 tons.8. The facility as in claim 1 , wherein the foundation comprises a concrete slab.9. The facility as in claim 1 , wherein the walls comprise interlocking blocks.10. The facility as in claim 1 , wherein the walls support a network of I-beams and the network of I-beams supports ceiling panels.11. The facility as in claim 1 , wherein the walls claim 1 , ceiling and foundation are at least about 4 feet thick.12. The facility as in claim 1 , wherein the walls claim 1 , ceiling and foundation include concrete and the concrete is selected from the ...

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Номер записи: 86

17-01-2019 дата публикации

IMPROVEMENTS IN AND RELATING TO CELL HARVESTING APPARATUS

Номер: US20190017005A1

Автор: Burger Krystal, Griffin Weston Blaine, Harris Dan, Lines Austin, Schuelke David

Принадлежит: GE HEALTHCARE BIO-SCIENCES CORP.

Disclosed herein is a cell harvesting instrument suitable for concentrating cells from a source suspension of cells and/or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump, at least one valve; and a processing kit removably insertable into the housing, said kit including a generally flat frame having or supporting plural sealed fluid paths arranged in a generally flat plane and such that fluids in the paths do not contact said mechanical elements, wherein at least portions of the fluid paths comprise flexible tubes, the outer surfaces of which are manipulateable by the or each fluid pump, to provide fluid flow in one or more of the paths and/or by the or each valve to restrict fluid flow in one or more of the paths. In an embodiment, the kit comprises also a fluid processing reservoir and a filter suitable for separating cells from fluid in said paths. A transfer mechanism for moving and weighing the fluid processing reservoir is disclosed also. 1. A cell harvesting processing kit for at least one of separating cells from fluids and washing cells , the kit comprising ,sealed fluid paths at least a portion of which are formed from flexible tubing suitable for being externally manipulated by mechanical elements,a fluid processing reservoir and a filter suitable for retaining cells whilst permeating fluids in said paths,wherein the fluid paths are supported by a generally flat supporting frame such that said paths, reservoir and filter are arranged in a generally flat plane.2. The kit of claim 1 , wherein said supporting frame includes through-apertures claim 1 , and said flexible tubing is arranged to extend across said apertures such that the tubing is accessible from both sides of the flat supporting frame to allow said external manipulation from both sides of the frame.3. The kit of claim 1 , wherein said fluid processing reservoir is in the form of a container which lies generally flat ...

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Номер записи: 87

17-01-2019 дата публикации

FILTER FOR FILTRATION OF NUCLEATED CELLS AND FILTRATION METHOD USING THE SAME

Номер: US20190017012A1

Автор: Banju Masaru, Kondo Takashi, MATSUMOTO Seiichi, Watanabe Junko

Принадлежит:

A filtration method for filtration of nucleated cells that includes providing a filter containing at least one of a metal and a metal oxide as a major component thereof and having a plurality of through-holes therein, and passing a liquid containing the nucleated cells through the filter. The diameter of an inscribed circle of each of the plurality of through-holes is smaller than the size of the nuclei of the nucleated cells, and the inscribed circle of each of the plurality of through-holes touches all sides defining an opening of the through-hole. 1. A method for filtration of nucleated cells , the method comprising:providing a filter comprising at least one of a metal and a metal oxide as a major component thereof and having a plurality of through-holes therein, each of the plurality of through-holes having a square shape, wherein a diameter of an inscribed circle of each of the plurality of through-holes is smaller than a size of nuclei of the nucleated cells, the inscribed circle of each of the plurality of through-holes touching all sides defining an opening of the through-hole; andpassing a liquid containing the nucleated cells through the filter.2. The filtration method according to claim 1 , wherein a ratio of the diameter of the inscribed circle of each of the plurality of through-holes to the size of the nuclei of the nucleated cells is 0.06 to 0.64.3. The filtration method according to claim 1 , wherein a ratio of the diameter of the inscribed circle of each through-hole to the size of the nuclei of the nucleated cells is 0.06 or more.4. The filtration method according to claim 1 , wherein a ratio of the diameter of the inscribed circle of each through-hole to the size of the nuclei of the nucleated cells is 0.07 or more.5. The filtration method according to claim 1 , wherein an immersion potential of one at least one of the metal and the metal oxide in phosphate-buffered saline is higher than 0.03 V with respect to a silver chloride reference electrode ...

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Номер записи: 88

17-01-2019 дата публикации

MECHANICAL LYSIS APPARATUS AND METHOD

Номер: US20190017013A1

Автор: Bickman Sarah R., Lochhead Michael J., Moll Kevin, Rowley Keagan B.

Принадлежит:

A mechanical lysis method includes (a) spinning a mixing head at a spin rate in a liquid sample, containing cells and beads, such that the mixing head cooperates with the beads to lyse the cells, wherein the spin rate exceeds a threshold rate associated with a predefined lysis efficiency, and (b) preventing the mixing head from spinning if the spin rate cannot be maintained above the threshold rate. A lysis apparatus includes (a) a receptacle for holding a container containing a liquid sample with cells and beads, (b) a mixing head configured to spin in the liquid sample, (c) a motor configured to spin the mixing head such that the mixing head cooperates with the beads to lyse the cells, and (d) a switch configured to prevent spinning of the mixing head at a spin rate lower than a threshold rate associated with a predefined lysis efficiency. 1. A mechanical lysis method , comprising:spinning a mixing head at a spin rate in a liquid sample, containing cells and beads, such that the mixing head cooperates with the beads to lyse the cells, the spin rate exceeding a threshold rate associated with a predefined lysis efficiency; andpreventing the mixing head from spinning if the spin rate cannot be maintained above the threshold rate.2. The mechanical lysis method of claim 1 , the step of spinning comprising:causing the beads to move; andbeating at least some of the cells with at least some of the beads to lyse the at least some of the cells.3. The mechanical lysis method of claim 1 , the step of spinning comprising beating at least some of the cells with the mixing head claim 1 , the beads enhancing at least one of (a) rate of the beating and (b) relative impact speed of the mixing head on the cells.4. The mechanical lysis method of claim 1 ,the step of spinning comprising spinning the mixing head inside a container holding the liquid sample; andfurther comprising actuating the mixing head with a motor disposed outside the container and mechanically coupled to the mixing ...

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Номер записи: 89

17-01-2019 дата публикации

Methods and devices for producing cellular suspensions from tissue samples

Номер: US20190017908A1

Автор: Mitchell FERGUSON, Ronald J. Pettis

Принадлежит: Becton Dickinson and Co

Aspects of the present disclosure include methods of producing a cellular suspension from a tissue sample by applying resonant acoustic energy to a container comprising the tissue sample in a manner sufficient to produce a cellular suspension from the tissue sample. Resonant acoustic mixers and kits for use in producing a cellular suspension from a tissue sample are also provided.

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Номер записи: 90

21-01-2021 дата публикации

Cell strainer

Номер: US20210017484A1

Автор: Kenjiro Taki

Принадлежит: Enplas Corp

A cell strainer causes fluid to flow out in a tube via a filter or causes fluid in the tube to flow in via the filter by an operation of a pipette engaged with the tube. The filter is formed into a shape such that the filter is entirely positioned in the tube when the cell strainer is engaged with the tube. A tip engaging hole press-fitted to a distal end of a tip for pipette is formed in the cell strainer. A flow passage communicating with the tip engaging hole is formed inside the cell strainer. The filter has a plurality of openings having similar shapes. The openings communicate between an inside of the flow passage and an internal space of the tube to filter out a substance larger than the openings among substances in the fluid.

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Номер записи: 91

16-01-2020 дата публикации

Recirculating Bioreactor

Номер: US20200017812A1

Автор: Jonathan Thon

Принадлежит: Platelet Biogenesis Inc

A bioreactor including a bioreactor body, wherein the bioreactor body includes a first substrate and an opposing second substrate, a pathway extending through the bioreactor body and being formed by a first channel defined in the first substrate and an opposing second channel defined in the second substrate, a first inlet for introducing a first fluid flow to the first channel, a second inlet for introducing a second fluid flow to the second channel, a first outlet for permitting the first fluid flow to exit the first channel, a second outlet for permitting the second fluid flow to exit the second channel, a membrane disposed in the pathway between the first and second channels and having a plurality of pores sized to selectively capture, in the first channel, a biological source material and to permit biological products to be collected from the bioreactor.

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Номер записи: 92

16-01-2020 дата публикации

METHOD FOR ACTIVATING AND EXPANDING ISOLATED T CELLS

Номер: US20200017830A1

Автор: Liu Kimberly, Shi Guixin, Wang Ying-Ting

Принадлежит:

A method for activating and expanding isolated T cells, the method including adding to isolated T cells ligands presenting microbubbles having a flexible lipid shell with an inner bubble wall enclosing a gas and an outer bubble wall conjugated to ligands capable of achieving cell contact dependent juxtacrine signaling on the isolated T cells; and adding to isolated T cells ligands presenting microbubbles having a flexible lipid shell with an inner bubble wall enclosing a gas and an outer bubble wall conjugated to an antigen capable of forming an immunological synapse (IS) with the T cells. 1. A method for activating and expanding isolated T cells , comprising:a) adding to isolated T cells ligands presenting microbubbles having a flexible lipid shell with an inner bubble wall enclosing a gas and an outer bubble wall conjugated to ligands capable of achieving cell contact dependent juxtacrine signaling on the isolated T cells; andb) adding to isolated T cells ligands presenting microbubbles having a flexible lipid shell with an inner bubble wall enclosing a gas and an outer bubble wall conjugated to an antigen capable of forming an immunological synapse (IS) with the T cells.2. The method of claim 1 , wherein the ligands presenting microbubbles are anti-CD3 and anti-CD28 conjugated microbubbles and the activating and expanding of the isolated T cells is used for preparing the T cells in vitro for adoptive cell transfer.3. The method of claim 2 , wherein preparing the T cells in vitro for adoptive cell transfer is for chimeric antigen receptor (CAR-T) cell therapy.4. The method of claim 1 , wherein the ligands presenting microbubbles are peptide/MHC and anti-CD28 conjugated microbubbles and the activating and expanding of the isolated T cells is used for preparing the T cells in vitro for adoptive cell transfer.5. The method according to claim 1 , further comprising incubating said cells with the ligands presenting microbubbles over a time span that is sufficient for ...

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Номер записи: 93

21-01-2021 дата публикации

PROCESSES FOR RECOVERING PRODUCTS FROM A CORN FERMENTATION MASH

Номер: US20210017547A1

Автор: BOOTSMA Jason

Принадлежит: FLINT HILLS RESOURCES, LP

Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product. 1. A process for recovering products from a fermentation mash , comprising:processing a ground corn product to produce a fermentation mash comprising ethanol;separating at least a portion of the ethanol from the fermentation mash to produce a whole stillage;separating the whole stillage to produce a fiber rich product and a filtrate;hydrolyzing the fiber rich product to produce a saccharification mash; andprocessing the saccharification mash to produce additional ethanol and a stillage protein product.2. The process of claim 1 , wherein the stillage protein product comprises about 15 wt % to about 35 wt % of yeast claim 1 , based on a dry weight of the stillage protein product.3. The process of claim 1 , wherein the stillage protein product comprises about 40 wt % to about 80 wt % of protein claim 1 , about 3 wt % to about 20 wt % of fat claim 1 , about 1 wt % to about 4 wt % of ash claim 1 , about 2 wt % to about 30 wt % of neutral detergent fibers claim 1 , about 1 wt % to about 15 wt % of acid detergent fibers claim 1 , and about 15 wt % to about 35 wt % of yeast claim 1 , based on a dry weight of the stillage protein product.4. The process of claim 1 , wherein the stillage protein product comprises about 47 wt % to about 57 wt % of protein claim 1 , about 3 wt % to about 5 wt % of fat claim 1 , about 1 wt % to about 3 wt % of ash claim 1 , about 4 wt ...

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Номер записи: 94

28-01-2016 дата публикации

Method and Apparatus for Gender Selection Based on PH

Номер: US20160024456A1

Автор: Penrose Lindsay L., Prien Samuel D.

Принадлежит:

The present invention includes methods and apparatus to separate X or Y-chromosome bearing sperm cells in a population by first placing the population of sperm cells at physiological pH environment, and simultaneously contacting the population of sperm cells with one or more additional sub-environments with different pH values. The exposure allows mobile sperm cells bearing X or Y-chromosome to migrate to the different pH sub-environments, wherein each cell only exposed or come in contact with one pH sub-environment. Finally, the collecting X or Y-chromosome enriched population of sperm cells is performed. 1. An apparatus for increasing the proportion of X or Y-chromosome bearing sperm cells in a population comprising:one or more containers, wherein the container comprises at least one main chamber; andtwo or more sub-chambers adjacent the main chamber, wherein the main chamber and sub-chambers are separated by a biocompatible mesh material, wherein the vessels are adapted to receive fluids comprising two or more separate pHs and the openings in the mesh permit the passage of sperm.2. The apparatus of claim 1 , wherein the biocompatible mesh material comprise openings of about 40-70 μm.3. The apparatus of claim 1 , wherein the biocompatible mesh material permits sufficient minor exchange of ions4. The apparatus of claim 1 , wherein the sufficient minor exchange is significant to permit mobility pathways for sperm cells.5. The apparatus of claim 1 , wherein the biocompatible mesh material prevent mass flow of fluid between the chambers claim 1 , but allows free movement of sperm cells and slow movement of fluids between chambers.6. The apparatus of claim 1 , wherein the pH of the chambers includes two or more pHs selected from 5 claim 1 , 6 claim 1 , 7 claim 1 , 7.5 claim 1 , 7.8 claim 1 , 8 claim 1 , 8.5 claim 1 , 9 and 9.5.7. The apparatus of claim 1 , wherein the biocompatible mesh vessel comprises an electrostatic charge claim 1 , static pressure claim 1 , or ...

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Номер записи: 95

28-01-2016 дата публикации

Bacterial identification

Номер: US20160024550A1

Автор: LaKeta Kemp, Mark Hayes, Paul Jones, Thomas Taylor

Принадлежит: Arizona Board of Regents of ASU

The present invention provides for separation of bacterial species and serotypes using electrophoretic methods.

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Номер записи: 96

22-01-2015 дата публикации

SYSTEM, APPARATUS AND METHOD FOR MATERIAL PREPARATION AND/OR HANDLING

Номер: US20150024480A1

Автор: Doebler Robert, Erwin Barbara, Hickerson Anna, IRVINE Bruce, Nadim Ali, Sterling James D., Talbot Ryan P., Woyski Denice

Принадлежит:

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency. 1110-. (canceled)111. An article to perform flow-through lysis by oscillation on material to be lysed , the article comprising:at least one wall forming at least one chamber having a first opening and at least a second opening spaced from the first opening, the first and the second openings to provide fluid communication into the chamber from an exterior thereof;a particulate lysing material received in the chamber, the particulate material including a plurality of particles sized to lyse a material to be lysed and having a combined particulate volume;a first filter received in the chamber between the first opening and the particulate material, the first filter having a plurality of apertures sized to substantially pass the material to be lysed and to retain the particulate material;a second filter received in the chamber between the second opening and the particulate material, the second filter having a plurality of apertures sized to pass the material to be lysed and to retain the particulate material, wherein the first filter and the second filter form a particulate retainment area therebetween having a volume sufficiently greater than said combined particulate volume to permit the plurality of particles to move relative to one another to ...

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Номер записи: 97

10-02-2022 дата публикации

NON-PLANAR AND NON-SYMMETRICAL PIEZOELECTRIC CRYSTALS AND REFLECTORS

Номер: US20220040733A1

Автор: Chitale Kedar, Dutra Brian, Gilmanshin Rudolf, III Thomas J., JR. Walter M., Kennedy, Lipkens Bart, Mealey Dane, Presz, Sokolowski David

Принадлежит:

An acoustophoretic device is disclosed. The acoustophoretic device includes an acoustic chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer includes a piezoelectric material driven by a voltage signal to create a multi-dimensional acoustic standing wave in the acoustic chamber emanating from a non-planar face of the piezoelectric material. A method for separating a second fluid or a particulate from a host fluid is also disclosed. The method includes flowing the mixture through an acoustophoretic device. A voltage signal is sent to drive the ultrasonic transducer to create the multi-dimensional acoustic standing wave in the acoustic chamber such that the second fluid or particulate is continuously trapped in the standing wave, and then agglomerates, aggregates, clumps, or coalesces together, and subsequently rises or settles out of the host fluid due to buoyancy or gravity forces, and exits the acoustic chamber. 1. An acoustic reflector , comprising a reflective portion for reflecting acoustic energy , the reflective portion including a non-planar face that is faceted.2. The reflector of claim 1 , further comprising a planar face opposite the non-planar face.3. The reflector of claim 2 , further comprising the reflective portion being composed of piezoelectric material.4. The reflector of claim 3 , further comprising the piezoelectric material being poled in a direction substantially perpendicular to the planar face of the reflector.5. The reflector of claim 1 , wherein the non-planar face of the reflector includes a shape that is defined by a step function or a smooth function.6. The reflector of claim 2 , wherein the non-planar face of the reflector includes a plurality of adjoining portions claim 2 , each of which are located at respective distances from a respective closest portion of the planar face claim 2 , the respective distances being different.7. The reflector of claim 6 , wherein the respective distance of each adjoining portion ...

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Номер записи: 98

10-02-2022 дата публикации

SEPARATION DEVICE AND METHOD FOR SEPARATING TO-BE-SEPARATED MATERIAL USING SAME

Номер: US20220041971A1

Автор: HATANAKA Daisuke, Hayashi Hisato, KANAKI Tatsuro, KIDA Katsuhiko

Принадлежит: NISSAN CHEMICAL CORPORATION

A separation device for separating a solid separation target contained in a suspension obtained by suspension culture of adherent cells by using a fine scaffold material, and a separation method. The separation device has a separation chamber having a first chamber and a second chamber, and the first chamber and the second chamber are divided by a mesh structure for separation. The mesh structure for separation has mesh-holes with a predetermined size to suppress passage of the separation target, and a mesh structure for separation is configured in the separation chamber such that the liquid in the aforementioned suspension has a vertically upward directional component in the advancing direction of the liquid when the liquid passes through the mesh-holes. 1. A separation device for separating a solid separation target contained in a suspension which is obtained by suspension culture of adherent cells by using a fine scaffold material , the separation device comprising:a separation chamber which comprises at least a first chamber and a second chamber, wherein the first chamber and the second chamber are divided by a mesh structure for separation, and an internal flow path is configured such that the liquid in the suspension medium that has entered the separation device moves from the first chamber through the mesh structure for separation to the second chamber and thereafter exits the separation device,the mesh structure for separation has mesh-holes with a predetermined size to suppress passage of the separation target, andthe mesh structure for separation is configured in the separation chamber such that the advancing direction of the liquid in the aforementioned suspension has a vertically upward directional component when the liquid passes through the mesh-holes.2. The separation device according to claim 1 , whereinthe suspension comprises a solid passing target to be passed through the mesh structure for separation together with the liquid, andthe mesh ...

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Номер записи: 99

10-02-2022 дата публикации

SEPARATION DEVICES, ASSOCIATED METHODS, AND SYSTEMS

Номер: US20220041976A1

Автор: Castle Jason William, De Anindya Kanti, Galligan Craig Patrick, Gettings Rachel Marie, Hale Jared Timothy, Kvam Erik Leeming, Misner Matthew Jeremiah, Morton Christine Angela, Pizzi Vincent Francis

Принадлежит:

A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid. 1. A method for clarifying a bioprocess fluid comprising particles suspended in a cell culture fluid , the method comprising:flowing an unclarified bioprocess fluid from a bioreactor through a plurality of mesofluidic channels of a separation device comprising a fluid inlet and a fluid outlet to separate at least a portion of particles of the unclarified bioprocess fluid to generate a substantially clarified bioprocess fluid; andcollecting the clarified bioprocess fluid from the fluid outlet of the separation device;wherein a residence time of the bioprocess fluid between the fluid inlet and fluid outlet of the separation device ranges from 10 minutes to 40 minutes, and wherein the separation device is operated at an angle less than 10° relative to a work surface.2. The method of claim 1 , wherein the separation device is operated at an angle less than or equal to 5° relative to the work surface.3. The method of claim 1 , wherein the separation device is operated at an angle of about 0° relative to the work surface.4. The method of claim 1 , wherein the particles comprise cells claim 1 , aggregated cells claim 1 , adhered cells on carriers claim 1 , diatomaceous earth claim 1 , resin beads claim 1 , or a combination thereof.5. The method of claim 1 , wherein the substantially clarified bioprocess fluid comprises biotherapeutically active products claim 1 ...

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Номер записи: 100