Настройки

Укажите год
-

Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

Подробнее
-

Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

Подробнее

Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Укажите год
Укажите год
Применить Всего найдено 10209. Отображено 100.
05-01-2012 дата публикации

Methods and compositions for modulating proline levels

Номер: US20120003207A1
Автор: Mike A. Clark
Принадлежит: ONCOPHARMACOLOGICS Inc

Methods and compositions for modulating amino acid levels in a subject are provided herein.

Подробнее
Номер записи: 1
19-04-2012 дата публикации

Recombinantly modified plasmin

Номер: US20120093799A1
Автор: Valery Novokhatny
Принадлежит: Individual

Polynucleotides and polypeptides relating to a recombinantly modified plasmin(ogen) molecule are provided. The plasmin(ogen) molecule has a single kringle domain N-terminal to the activation site present in the native human plasminogen molecule, combined such that no foreign sequences are present, and exhibits lysine-binding and significant enzymatic characteristics associated with the native enzyme

Подробнее
Номер записи: 2
17-05-2012 дата публикации

Recombinant protein of fibroblast growth factor having adhesive activity for stem cells and method for culturing stem cells using the same

Номер: US20120122156A1
Автор: Min Han, Sang-Heon Kim, Soo Hyun Kim, Young Mee Jung
Принадлежит: Korea Advanced Institute of Science and Technology KAIST

The present invention relates to a recombinant protein of a fibroblast growth factor (FGF) having an adhesive activity for stem cells and a method for culturing stem cells using the same. More particularly, the present invention relates to a recombinant protein having an adhesive activity for stem cells by fusion of a polypeptide linker at amino terminal of FGF, and a method for culturing stem cells using immobilized FGF comprising: fixing the recombinant protein in a culture vessel with a hydrophobic surface using amino terminal of the polypeptide linker, adhering stem cells on the recombinant protein-fixed culture vessel, and culturing the stem cells.

Подробнее
Номер записи: 3
14-06-2012 дата публикации

Sorption reinforced catalytic coating system and method for the degradation of threat agents

Номер: US20120148850A1
Автор: John P. Puglia, Robert A. Reinstein, Steven E. Weiss, Tomasz Modzelewski, Yongwoo Lee
Принадлежит: Foster Miller Inc

Sorption reinforced catalytic coating system for the degradation of threat agents including a synzyme coating about a material, the synzyme coating having bucket-shaped molecules for the sorption and degradation of the threat agents. A binding agent is configured for synzyme immobilization to maximize loading and retention of the synzyme coating on the material.

Подробнее
Номер записи: 4
09-08-2012 дата публикации

Analytical method and sensor

Номер: US20120198920A1
Автор: Björn Ingemarsson, Camilla Käck, Julien Saint-Guirons, Kerstin Lundell
Принадлежит: ATTANA AB

There is disclosed an analytical method and a sensor suitable for carrying out the method. More specifically, there is disclosed a method for preparing a mass sensitive chemical sensor capable of detecting binding analyte species to a surface comprising cells.

Подробнее
Номер записи: 5
16-08-2012 дата публикации

Apparatus and process for production of an encapsulated cell product

Номер: US20120208255A1
Автор: Christine A. Singer, John H. Evans, Iv, Lisa Beckler Andersen
Принадлежит: Geosynfuels LLC

A process for production of an encapsulated cell product, the process comprises the steps of concentrating cells from a propagation medium using a tangential flow filtration system. Mixing the concentrated cells with an encapsulation medium to form a cell encapsulation mixture. Polymerizing, gelling, or cross-linking the cell encapsulation mixture to form an encapsulated cell product.

Подробнее
Номер записи: 6
04-10-2012 дата публикации

Probiotic Compositions, Methods and Apparatus for Their Administration

Номер: US20120247993A1
Автор: Jenni Sofjan, Ken Palazzi, Kristel Wallis, Mark Styan, Patricia Conway, Yin Li
Принадлежит: Unistraw Patent Holdings Ltd

A probiotic composition including a probiotic microorganism embedded within a matrix, the matrix substantially maintaining the viability of said microorganisms. The matrix releases said microorganisms into and upon contact with a liquid carrier. The invention includes methods for manufacturing the composition, particular forms of the composition ( 2 ) and as apparatus for administration.

Подробнее
Номер записи: 7
31-01-2013 дата публикации

Thermostable sucrose phosphorylase

Номер: US20130029384A1
Автор: An Cerdobbel, Tom Desmet, Wim Soetaert
Принадлежит: Universiteit Gent

The present invention relates to a sucrose phosphorylase from Bifidobacterium adolescentis which is useful as a biocatalyst in carbohydrate conversions at high temperatures. Indeed, the biocatalysts of the present invention are enzymatically active for a time period of at least 16 h and up to 1 to 2 week(s) at a temperature of at least 60° C. The biocatalysts of the present invention are: a) immobilized on an enzyme carrier, or b) are part of a cross-linked enzyme aggregate (CLEA), and/or c) are mutated, and/or d) are enzymatically active in the continuous presence of their substrate.

Подробнее
Номер записи: 8
14-03-2013 дата публикации

Coatings Containing Polymer Modified Enzyme For Stable Self-Cleaning Of Organic Stains

Номер: US20130065291A1
Автор: Andreas Buthe, Hongfei Jia, Liting Zhang, Masahiko Ishii, Minjuan Zhang, PING Wang, Songtao Wu, Xueyan Zhao
Принадлежит: Department of Bioproducts and Biosystems Engineering of University of Minnesota, Toyota Motor Corp, Toyota Motor Engineering and Manufacturing North America Inc

Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Подробнее
Номер записи: 9
28-03-2013 дата публикации

Device for Forming Drops in a Microfluidic Circuit

Номер: US20130078164A1
Автор: Charles Baroud, Remi Dangla
Принадлежит: ECOLE POLYTECHNIQUE

The invention relates to a device ( 1 ) for forming droplets in a microfluidic circuit, the device comprising a chamber ( 3 ) containing a first fluid and defined by two opposite walls ( 10, 11 ) that diverge relative to each other in at least one given direction, and a microchannel ( 8 ) containing a second fluid and leading into a zone of said chamber ( 3 ) that is upstream relative to the given direction, the outlet of the microchannel ( 8 ) into the chamber ( 3 ) constituting an enlargement in the flow section for the second fluid, and the enlargement giving rise to droplets ( 14 ) of the second fluid forming within the first fluid.

Подробнее
Номер записи: 10
11-04-2013 дата публикации

THERAPEUTIC NUCLEASE COMPOSITIONS AND METHODS

Номер: US20130089546A1
Автор: Elkon Keith, Hayden-Ledbetter Martha, Ledbetter Jeffrey A., Sun Xizhang
Принадлежит: UNIVERSITY OF WASHINGTON

Hybrid nuclease molecules and methods for treating an immune-related disease or disorder in a mammal, and a pharmaceutical composition for treating an immune-related disease in a mammal. 1. A hybrid nuclease molecule comprising a first nuclease domain and a modified Fc domain , wherein the first nuclease domain is operatively coupled to the Fc domain.263.-. (canceled)64. The hybrid nuclease molecule of wherein the Fc domain is modified to decrease binding to Fcγ receptors or complement proteins or both.65. The hybrid nuclease molecule of which has a reduced effector function selected from the group consisting of opsonization claim 64 , phagocytosis claim 64 , complement dependent cytotoxicity claim 64 , and antibody-dependent cellular cytotoxicity.66. The hybrid nuclease molecule of claim 1 , further comprising a second nuclease domain operatively coupled to the Fc domain.67. The hybrid nuclease molecule of claim 1 , wherein the first nuclease domain is operatively coupled to the Fc domain via a first linker domain68. The hybrid nuclease molecule of claim 67 , wherein the first linker domain is a polypeptide linker claim 67 , such as a gly-ser linker.69. The hybrid nuclease molecule of claim 66 , wherein the second nuclease domain is operatively coupled to the Fc domain via a second linker domain.70. The hybrid nuclease molecule of claim 69 , wherein the second linker domain is a polypeptide linker claim 69 , such as an NLG peptide.71. The hybrid nuclease molecule of claim 1 , wherein the first nuclease domain comprises an RNase.72. The hybrid nuclease molecule of claim 71 , wherein the RNase is a human RNase claim 71 , such as a human pancreatic RNase A.73. The hybrid nuclease molecule of claim 1 , wherein the Fc domain comprises a human immunoglobulin Fc domain claim 1 , such as a human IgG1 Fc domain.74. The hybrid nuclease molecule of claim 73 , wherein the Fc domain comprises a hinge domain claim 73 , a CH2 domain and a CH3 domain.75. The hybrid nuclease ...

Подробнее
Номер записи: 11
18-04-2013 дата публикации

Compositions for transfection of biomolecules into cells

Номер: US20130095124A1
Автор: Peter Grandics, Susan Szathmary
Принадлежит: Individual

The present invention is directed to new compositions that are described for the simultaneous, controlled dose delivery of a variety of biomolecules into phagocytic cells. Such a composition is a biologically active composition comprising: (1) at least one of the following biologically active components: (a) a nucleic acid or a derivative thereof; (b) a nucleoside, nucleotide, or a derivative of a nucleoside or nucleotide; (c) a peptide, protein, or a derivative of a peptide or protein; (d) a lipopolysaccharide or a derivative thereof; (e) a peptidoglycan or a derivative thereof; (f) a carbohydrate or a derivative thereof; (g) a lipid or a derivative thereof; (h) a lipopeptide or a derivative thereof; (i) a metal ion; (j) a thiol; (k) an antibiotic or a derivative thereof; (I) a vitamin or a derivative thereof; (m) a bioflavonoid or a derivative thereof; (n) an antioxidant or a derivative thereof; (o) an immune response modifier; (p) an antibody; (q) a biologically active nonmetal; (r) histamine or an antihistamine; and (s) a kinase inhibitor; and (2) at least one carrier effective to deliver the composition to a phagocytic cell such that the biologically active component is taken up by the phagocytic cell and influences its biological activity.

Подробнее
Номер записи: 12
25-04-2013 дата публикации

Method of retrovirus storage

Номер: US20130102048A1
Автор: Hideto Chono, Hiromi Okuyama, Ikunoshin Kato, Junichi Mineno, Kiyozo Asada, Yasushi Katayama
Принадлежит: Takara Bio Inc

A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.

Подробнее
Номер записи: 13
02-05-2013 дата публикации

Support for protein immobilization, immobilized protein, and methods for producing the same

Номер: US20130109072A1
Автор: Chisato Sekikawa, Fujio Mizukami, Hideaki Togashi, Miki Egami, Naoki Tahara, Seigo Ono, Shuzo Kojima, Takayuki Nara, Tatsuo Tsunoda
Принадлежит: JGC Corp, National Institute of Advanced Industrial Science and Technology AIST

A support for enzyme immobilization is described, which is for immobilizing enzymes of various molecular sizes and also for, due to the modification of the surface silanol groups of porous silica particles, for immobilizing various kinds of enzymes, and enables the design of an immobilized enzyme, which exhibits an activity equivalent to that of the corresponding non-immobilized enzyme and withstands repeated use. A method for producing the support is also described. The support includes porous silica particles having an interparticle void structure therein, characterized in that the porous silica particles have a specific average particle size, a specific surface area, a specific pore volume, a specific pore size distribution and a specific porosity and have a substituent containing an organic group or an amino group on the surface thereof. An immobilized protein obtained by immobilizing a protein on the above support is also described.

Подробнее
Номер записи: 14
09-05-2013 дата публикации

Selective photo-induced protein immobilization using bovine serum albumin

Номер: US20130115558A1
Автор: Wan Joong Kim
Принадлежит: Electronics and Telecommunications Research Institute ETRI

Provided is a biomaterial immobilizing method including immobilizing a bovine serum albumin on a substrate, providing a biomaterial on the substrate immobilized with the bovine serum albumin, and irradiating an ultraviolet light onto the substrate provided with the bovine serum albumin and the biomaterial to immobilize the biomaterial selectively on the substrate immobilized with the bovine serum albumin.

Подробнее
Номер записи: 15
09-05-2013 дата публикации

BACTERIUM-BASED MICROROBOT INCLUDING MAGNETIC PARTICLES

Номер: US20130116621A1
Автор: PARK Jong Oh, PARK Suk Ho, Park Sung Jun
Принадлежит: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY

Provided is a bacterium-based microrobot, wherein bacteria are attached to a part of a surface of a microstructure including at least one or more magnetic particle, for actuating a bacterium-based microrobot more effectively. 1. A bacterium-based microrobot comprising a microstructure comprising one or more magnetic particles , wherein the bacteria are attached to a part of the surface of the microstructure.2. The bacterium-based microrobot of claim 1 , wherein the magnetic particles comprise a ferromagnetic substance.3. The bacterium-based microrobot of claim 1 , wherein the microstructure comprises a drug and the magnetic particles are dispersed in the drug.4. The bacterium-based microrobot of claim 1 , wherein the microstructure has a core/shell structure.5. The bacterium-based microrobot of claim 1 , wherein the microstructure has a multilayer structure.6. The bacterium-based microrobot of claim 1 , further comprising a drug-encapsulated bead in which a drug is encapsulated.7. The bacterium-based microrobot of or claim 1 , further comprising a therapeutic bacterium-encapsulated bead in which therapeutic bacteria are encapsulated.8. The bacterium-based microrobot of claim 3 , wherein the drug is an anticancer drug claim 3 , a radionuclide claim 3 , a therapeutic drug for ischemic diseases claim 3 , or a therapeutic drug for infarction.9. The bacterium-based microrobot of claim 1 , wherein the bacteria are selectively attached to the part of the surface of the microstructure.10. The bacterium-based microrobot of claim 9 , wherein the bacteria are selectively attached to the part of the surface of the microstructure by controlling surface energy of the part of the surface of the microstructure.11. The bacterium-based microrobot of claim 10 , wherein if the surface of the microstructure is made of a hydrophilic material claim 10 , the part of the surface of the microstructure is modified to be hydrophobic claim 10 , or if the surface of the microstructure is made of ...

Подробнее
Номер записи: 16
16-05-2013 дата публикации

NANOSTRUCTURED COMPOSITE MATERIAL AND BIOSENSOR COMPRISING IT

Номер: US20130122531A1
Автор: Chaniotakis Nikolaos, Chatzimarinaki Maria, Stavgianoudaki Vassiliki, TSOUKALIS Achilleas, Vamvakaki Vassiliki
Принадлежит: Achilleas Tsoukalis

A nanostructured composite is provided which comprises a matrix comprising carbon nanofibers, and a biochemical receptor, especially an enzyme, immobilized in the matrix, where the carbon nanofibers have been subject to an oxidation treatment. In a preferred embodiment, the nanostructured composite further comprises a biomimetically synthesized silica matrix. 1. Nanostructured composite material , especially for application in biosensors , comprising:a silica matrix precipitate on a scaffold comprising a polymer or a peptide, carbon nanofibres; anda biochemical receptor immobilized on said carbon nanofibers, wherein said carbon nanofibers are activated.2. Nanostructured composite material according to claim 1 , wherein the biochemical receptor is selected from the group consisting of isolated enzymes claim 1 , microorganisms claim 1 , immunosystems claim 1 , tissues claim 1 , organelles claim 1 , whole cells claim 1 , antibodies claim 1 , nucleic acids and combinations thereof.3. Nanostructured composite material according to claim 1 , wherein the amount of acidic surface functional groups in the activated carbon nanofibers is at least 6*10mol/gram.4. (canceled)5. Nanostructured composite material according to claim 1 , wherein the scaffold material is selected from the group consisting of poly-L-lysine claim 1 , polyethylenimine claim 1 , polyallylamine claim 1 , cellulose claim 1 , R5 peptide claim 1 , poly-L-arginine claim 1 , poly-L histidine claim 1 , lysine oligomers claim 1 , poly(allylamine hydrochloride).6. Nanostructured composite material according to claim 1 , wherein porin molecules are incorporated in the silica matrix.7. Method for producing a nanostructured composite material according to claim 1 , comprising:activating carbon nanofibers by treatment with an oxidizing agent;adjusting the pH value at the surface of the carbon nanofibers to 7;immobilizing a biochemical receptor on the activated carbon nanofibers; andoptionally, depositing a scaffold ...

Подробнее
Номер записи: 17
23-05-2013 дата публикации

Enzyme-fiber matrix composite of three-dimensional network structure, preparation method thereof, and use thereof

Номер: US20130130284A1
Автор: Hyeong-Seok Kim, Jungbae Kim
Принадлежит: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Disclosed is a composite of enzyme and fiber matrix with three-dimensional structure. The composite of enzyme and fiber matrix with three-dimensional structure includes a significantly large amount of an enzyme loaded in and immobilized in/onto a matrix when compared to conventional composites. In addition, the immobilized enzyme is prevented from leaching from the matrix when an external impact is applied to the composite of enzyme and fiber matrix with three-dimensional structure. Therefore, the stability of the composite of enzyme and fiber matrix with three-dimensional structure of the present invention is maintained even after a long period passes since a remarkably great amount of enzymes compared with a known composite can be supported and immobilized to a matrix, and the immobilized enzyme is not easily released by an external impact. In addition, it is possible to stably immobilize a great amount of enzymes even if a functional group covalently bonding to enzymes is hardly present on the surface of fiber. Therefore, it is possible to remarkably improve performance by using the composite of enzyme and fiber matrix with three-dimensional structure of the present invention in a biosensor, a bio-fuel cell and the like, compared with the case using a known matrix composite.

Подробнее
Номер записи: 18
23-05-2013 дата публикации

SUSTAINED RELEASE MICROPARTICLES AND SUSTAINED RELEASE MICROPARTICLE-CONTAINING PREPARATIONS

Номер: US20130130910A1
Автор: Hori Youichi, Ishimori Satoru, Sato Mitsuyoshi
Принадлежит:

[Problem] 121-. (canceled)22. Sustained release microparticles comprising a supported substance , microgranular form having a hollow structure and coating agent which is at least one member selected from the group consisting of thermosetting resins , characterized in that the microgranular form has an average particle size within a range from 20 to 300 μm.23. Sustained release microparticles according to claim 22 , which contain claim 22 , based on the weight of the sustained release microparticles claim 22 , from 0.1 to 95 wt % of the supported substance claim 22 , from 0.1 to 90 wt % of the microgranular form claim 22 , and from 0.1 to 60 wt % of the coating agent.24. Sustained release microparticles according to claim 22 , in which the microgranular form has a floating ratio on water of from 20 to 95% and water absorption of from 10 to 170%.25. Sustained release microparticles according to claim 22 , in which the microgranular form is of hollow glass microspheres.26. Sustained release microparticles according to claim 22 , in which the microgranular form is at least one member selected from the group consisting of foamed pearlite claim 22 , foamed glass claim 22 , fly ash and hollow microballoon.27. Sustained release microparticles according to claim 22 , in which the microgranular form has an average particle size within a range from 20 to 150 μm.28. Sustained release microparticles according to claim 22 , in which the microgranular form has an average particle size within a range from 20 to 80 μm.29. Sustained release microparticles according to claim 22 , in which the supported substance is at least one member selected from the group consisting of agrochemically active component claim 22 , coloring matter claim 22 , flavor and fragrance claim 22 , functional substance claim 22 , medicament claim 22 , fertilizer component claim 22 , enzyme and physiologically active substance.30. Sustained release microparticles according to claim 29 , in which the supported ...

Подробнее
Номер записи: 19
23-05-2013 дата публикации

Textile-Templated Electrospun Anisotropic Scaffolds for Tissue Engineering and Regenerative Medicine

Номер: US20130131830A1
Автор: Brookstein David, Govindaraj Muthu, Lelkes Peter I., Senel H. Gozde
Принадлежит: PHILADELPHIA UNIVERSITY

The present invention includes an anisotropic scaffold, which is prepared by electrospinning a solution of matrix material upon a textile template. The present invention further includes a method of preparing such scaffold. The anisotropic scaffold of the invention finds use in tissue engineering and regenerative medicine. 1. A composition comprising an anisotropic scaffold , wherein said scaffold is prepared by electrospinning a solution of matrix material in a solvent upon a textile template.2. The composition of claim 1 , wherein said matrix material comprises a protein or a polymer.3. (canceled)4. The composition of claim 2 , wherein said polymer is selected from the group consisting of poly(urethane) claim 2 , poly(siloxane) claim 2 , poly(ethylene) claim 2 , poly(vinyl pyrrolidone) claim 2 , poly(2-hydroxy ethyl methacrylate) claim 2 , poly(N-vinyl pyrrolidone) claim 2 , poly(methyl methacrylate) claim 2 , poly(vinyl alcohol) claim 2 , poly(acrylic acid) claim 2 , polyacrylamide claim 2 , poly(ethylene-co-vinyl acetate) claim 2 , poly(ethylene glycol) claim 2 , poly(methacrylic acid) claim 2 , polylactide claim 2 , polyglycolide claim 2 , poly(lactide-co-glycolide) claim 2 , polyanhydride claim 2 , polyorthoester claim 2 , polycarbonate claim 2 , and combinations and co-polymers thereof.56-. (canceled)7. The composition of claim 2 , wherein said polymer is co-span with at least one compound selected from the group consisting of gelatin claim 2 , elastin and mixtures thereof.8. (canceled)9. The composition of claim 2 , wherein said polymer is co-span with at least one conductive polymer.10. The composition of claim 9 , wherein said conductive polymer is selected from the group consisting of polyfluorene claim 9 , polyphenylene claim 9 , polypyrene claim 9 , polyazulene claim 9 , polynaphthalene claim 9 , polypyrrole claim 9 , polycarbazole claim 9 , polyindole claim 9 , polyazepine claim 9 , polyaniline claim 9 , polythiophene claim 9 , poly(3 claim 9 ,4- ...

Подробнее
Номер записи: 20
30-05-2013 дата публикации

ARTIFICIAL BLOOD VESSEL AND METHOD OF MANUFACTURING THEREOF

Номер: US20130137156A1
Автор: MIYAKE Hideyuki, Morita Ikuo
Принадлежит: Dai Nippon Printing Co., Ltd.

An artificial tissue capable of carrying necessary nutrients for maintaining activities of cells and tissues, and method of manufacturing artificial blood vessel. A plurality of forms of blood vessels are extracted from an image of living tissue and made into blood vessel form image. Each of the blood vessel forms of the blood vessel form image is adjusted and a blood vessel formation pattern is formed. A blood vessel cell culturing pattern of forming is formed, in a cell culturing layer. The blood vessel cell culturing pattern includes: a cell adhesion portion having adhesive properties with blood vessel cell and formed to the blood vessel formation pattern; and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties for inhibiting adhesion with a blood vessel cell and formed in an area other than the cell adhesion portion. A blood vessel cell is adhered to the cell adhesion portion, and cultured into tissue. 2. The blood vessel cell culturing pattern base material according to claim 1 , wherein the cell adhesion-inhibiting portion is formed in an auxiliary pattern formed in the blood vessel pattern and formed so as not to inhibit an adhesion between the blood vessel cells adjoining to one another sandwiching the cell adhesion-inhibiting portion which corresponds to the auxiliary pattern.3. The blood vessel cell culturing pattern base material according to claim 2 , wherein a width of the blood vessel pattern is wider than 100 μm.4. The blood vessel cell culturing pattern base material according to claim 3 , wherein the auxiliary pattern is formed in line in the blood vessel pattern.5. The blood vessel cell culturing pattern base material according to claim 4 , wherein a line width of the auxiliary pattern is in the range from 0.5 μm to 10 μm.6. A vascular endothelial cell pattern base material comprising a base material and a vascular endothelial cell provided on the base material in such a way that the cell can be peeled off claim 4 , ...

Подробнее
Номер записи: 21
06-06-2013 дата публикации

Enzyme/carbon structure complex, method for preparing same, and use thereof

Номер: US20130143130A1
Автор: Jungbae Kim
Принадлежит: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Disclosed is a composite of enzyme and carbon structure. In the composite of enzyme and carbon structure, a significantly large amount of an enzymeis immobilized on the surface of carbon structures without the formation of chemical bonds (particularly, covalent bonds) between the enzyme molecules and the carbon structures. Since the surface of the carbon structures does not need to be modified to form chemical bonds, the electrical conductivity of the composite of enzyme and carbon structure is not reduced and the stability of the composite is maintained high even after the passage of a long time in various environments. Therefore, the use of the composite of enzyme and carbon structure enables the fabrication of various devices, such as biosensors and biofuel cells, with markedly improved performance as compared to the use of conventional enzyme/carbon structure composites.

Подробнее
Номер записи: 22
13-06-2013 дата публикации

EMULSION-DERIVED PARTICLES

Номер: US20130149730A1
Автор: Berber Isak Bartholomeus, Brady Dean, Gardiner Neil Stockenstrom, Jordaan Justin, Simpson Clinton
Принадлежит: CSIR

An emulsion-derived particle comprises a lattice of polymeric strands cross-linked by means of a cross-linking agent, and interstitial openings adjacent and around the strands. Functional groups are provided on the lattice and proteins and/or modified proteins can react with these, thereby to be bonded to the lattice and hence immobilised. 1. An emulsion-derived particle , which comprises a support lattice of polyethyleneimine strands cross-linked by means of a cross-linking agent , with the polyethyleneimine strands thus forming a network backbone of the support lattice , interstitial openings adjacent and around the strands , and aldehyde functional groups on the support lattice and with which proteins and/or modified proteins can react , thereby to be bonded to the support lattice and hence immobilised.2. A particle according to claim 1 , wherein the aldehyde functional groups are present on the polyethyleneimine claim 1 , with bonding of the proteins and/or modified proteins to the polyethyleneimine being effected through bonding.3. A particle according to claim 1 , which includes at least one protein and/or modified protein bonded to the support lattice by means of the aldehyde functional groups claim 1 , thereby being immobilised.4. A particle according to claim 3 , wherein a plurality of different proteins and/or a plurality of different modified proteins claim 3 , are immobilised therein.5. A particle according to claim 1 , which includes an adjunct entrapped within the support lattice.6. A particle according to claim 5 , wherein the adjunct is selected from the group consisting of a co-factor claim 5 , a modified co-factor claim 5 , a chemical mediator claim 5 , magnetite and a magnetic substance.720.-. (canceled)21. A particle according to claim 1 , wherein the support lattice is fibrous.22. An emulsion-derived particle claim 1 , which comprises a network backbone of polyethyleneimine strands cross-linked by means of a cross-linking agent claim 1 , with ...

Подробнее
Номер записи: 23
04-07-2013 дата публикации

CULTURE METHOD AND CULTURE DEVICE

Номер: US20130171712A1
Автор: FUKUDA Junji, Kakegawa Takahiro, MOCHIZUKI Naoto, SUZUKI Hiroaki
Принадлежит: University of Tsukuba

Provided are a culture method and a culture device for efficiently producing cells and/or cell tissue suitable for medication applications. A culture method of culturing cells adhered onto an electrode layer, the method including: a first step of adhering the cells onto the electrode layer and culturing the cells thereon, the electrode layer being coated with an oligopeptide including: a terminal amino acid; a cell adhesive sequence; and an alternating sequence to be bound to the one end side of the cell-adhesive sequence, the alternating sequence including a plurality of acidic amino acids and a plurality of basic amino acids, being alternately bound to each other one by one; and a second step of applying, to the electrode layer having the cells adhered thereonto, an electrical potential inducing reductive desorption of the oligopeptide, to thereby detach the cells from the electrode layer. 1. A culture method of culturing cells adhered onto an electrode layer , the method comprising: a terminal amino acid forming one end thereof to be bound to the electrode layer via thiolate;', 'a cell adhesive sequence forming another end thereof and having a cell-adhesive amino acid sequence; and', 'an alternating sequence to be bound to the one end side of the cell-adhesive sequence, the alternating sequence including a plurality of acidic amino acids and a plurality of basic amino acids, being alternately bound to each other one by one; and, 'the electrode layer being coated with an oligopeptide including, 'a first step of adhering the cells onto the electrode layer and culturing the cells thereon,'}a second step of applying, to the electrode layer having the cells adhered thereonto, an electrical potential inducing reductive desorption of the oligopeptide, to thereby detach the cells from the electrode layer.2. The culture method according to claim 1 , wherein the electrode layer is formed on a surface of a porous film held in a state of being suspended in a culture medium.3 ...

Подробнее
Номер записи: 24
08-08-2013 дата публикации

Technology and method to study microbial growth and adhesion to host-related surfaces and the host-microbiota interaction

Номер: US20130203161A1
Автор: Barbara Vanhoecke, Massimo Marzorati, Pieter Van Den Abbeele, Sam Possemiers, Tom Van De Wiele, Willy Verstraete
Принадлежит: Universiteit Gent

A method is provided for co-culturing viable cells and microorganisms for at least 48 hours in which an adhesion module is provided including a basal compartment and a luminal compartment separated by a semi-permeable membrane, and a continuous or semi-continuous flow of fresh medium is applied to the basal compartment.

Подробнее
Номер записи: 25
29-08-2013 дата публикации

Markers of Endothelial Progenitor Cells and Uses Thereof

Номер: US20130224116A1
Автор: Bonder Claudine Sharon, Lopez Angel Fransciso, Talbo Gert Hoy
Принадлежит: TransBio Ltd.

The present invention provides markers of endothelial progenitor cells (EPCs) and use of those markers and reagents that bind thereto to detect EPC cells or diagnose, prognose, treat or prevent EPC-associated conditions. 1. A method for detecting an endothelial progenitor cell (EPC) comprising determining the level of expression of a nucleic acid or protein set forth in Table 1 , or a nucleic acid or a protein having at least about 70% identity thereto , in , on or secreted from a cell , wherein an increased level of expression of a nucleic acid or protein set forth in Table 1 or a nucleic acid or protein having at least about 70% identity thereto compared to another cell type is indicative of an EPC.2. A method according to claim 1 , wherein the nucleic acid or protein is expressed in claim 1 , on or secreted from EPCs at a level at least 1.5 fold greater or 2 fold greater or 3 fold greater or 4 fold greater or 5 fold greater than in claim 1 , on or secreted by human umbilical cord vascular endothelial cells (HUVECs).3. A method according to claim 2 , wherein the nucleic acid or protein is expressed in claim 2 , on or secreted by non-adherent CD133 EPCs at a level at least 1.5 fold greater or 2 fold greater or 3 fold greater or 4 fold greater or 5 fold greater than in claim 2 , on or secreted by HUVECs.4. A method for detecting an endothelial progenitor cell (EPC) comprising determining the level of expression of a protein that is a cell adhesion molecule or a nucleic acid encoding the protein as set forth in Table 2 claim 2 , or a nucleic acid or protein having at least about 70% identity thereto claim 2 , in claim 2 , on or secreted from a cell claim 2 , wherein an increased level of expression of a nucleic acid or protein set forth in Table 2 or a nucleic acid or protein having at least about 70% identity thereto compared to another cell type is indicative of an EPC.5. A method for detecting an endothelial progenitor cell (EPC) comprising determining the level ...

Подробнее
Номер записи: 26
29-08-2013 дата публикации

METHOD FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS

Номер: US20130224799A1
Автор: Korfhage Christian
Принадлежит: QIAGEN GmbH

A method is disclosed for improved isothermal amplification of nucleic acids comprising the step of release of an essential component from a matrix under predetermined conditions. Furthermore, the invention relates to a kit comprising mesophilic enzyme and a matrix with embedded essential components for isothermal amplification. A composition comprising a matrix and a mesophilic enzyme and a method for embedding a mesophilic enzyme are disclosed as well. 1. A method for isothermal amplification of nucleic acids comprising the steps of: a) a mesophilic enzyme for amplifying nucleic acids under isothermal conditions;', 'b) one or more primer for amplifying a target nucleic acid;', 'c) dNTPs and/or NTPs;', 'd) essential co-factors and/or reagents of the enzyme for amplifying nucleic acids under isothermal conditions;', 'e) at least one target nucleic acid;', 'wherein at least one of the reaction components a) to e) is embedded in a matrix, wherein said matrix disintegrates at temperatures of 60° C. or more, preferably 65° C. or more;, 'i) providing at least the following reaction componentsii) incubating the reaction components under conditions which result in the disintegration of said matrix in order to obtain the reaction mixture;iii) incubating the reaction mixture under conditions suited for the isothermal amplification reaction;wherein the incubation temperature under step ii) is 1° C. to 50° C. higher than the incubation temperature of step iii), preferably 5° C. to 25 ° C. higher, more preferably 10° C. to 20° C. higher.2. The method according to claim 1 , wherein the mesophilic enzyme for amplifying nucleic acids has an optimum temperature of 20° C. to 70° C. claim 1 , preferably 30° C. to 65° C. claim 1 , more preferably 37° C. to 60° C.3. The method according to claim 1 , wherein the matrix forms a hydrogel.4. The method according to claim 1 , wherein the matrix is selected from the group comprising polysaccharides (agarose claim 1 , low-melting agarose ...

Подробнее
Номер записи: 27
29-08-2013 дата публикации

ELECTRODE HAVING ENZYME CRYSTALS IMMOBILIZED THEREON, METHOD FOR PRODUCING ELECTRODE HAVING ENZYME CRYSTALS IMMOBILIZED THEREON, AND BIOLOGICAL FUEL CELL AND BIOSENSOR PROVIDED WITH ELECTRODE HAVING ENZYME CRYSTALS IMMOBILIZED THEREON

Номер: US20130224824A1
Автор: Mikawa Tsutomu, Nakaoki Yuichiro, Shigemori Yasushi
Принадлежит:

An objective of the present invention is to establish a technique for making it possible to immobilize an enzyme on an electrically conductive base material in a uniformly, high density, and constantly aligned orientation, for the purpose of constructing an enzyme electrode having improved electrode performance. An electrode having enzyme crystals immobilized thereon, the electrode being provided with an electrically conductive base material that can be connected to an external circuit and enzyme crystals that serve as an electrode catalyst, wherein the enzyme crystals are immobilized on the electrically conducive base material; a method for producing an electrode having enzyme crystals immobilized thereon; and a biological fuel cell and a biosensor which are provided with an electrode having enzyme crystals immobilized thereon. 1. An electrode having enzyme crystals immobilized thereon , provided with an electroconductive base material that can be connected to an external circuit and enzyme crystals serving as an electrode catalyst , the enzyme crystals being immobilized on the electroconductive base material.2. The electrode having enzyme crystals immobilized thereon as set forth in claim 1 , configured to be a microelectrode in which the enzyme crystals have been immobilized on a microfabricated electroconductive base material.3. The electrode having enzyme crystals immobilized thereon as set forth in claim 1 , wherein the enzyme crystals are crystals of an enzyme for catalyzing an oxidation reaction.4Acinetobacter calcoaceticus.. The electrode having enzyme crystals immobilized thereon as set forth in claim 3 , wherein the enzyme crystals are crystals of pyrroloquinoline quinone-dependent glucose dehydrogenase from5Acinetobacter calcoaceticus. The electrode having enzyme crystals immobilized thereon as set forth in claim 4 , wherein the pyrroloquinoline quinone-dependent glucose dehydrogenase from has the amino acid sequence of any of (A) to (C) below.(A) The ...

Подробнее
Номер записи: 28
29-08-2013 дата публикации

LIGAND FUNCTIONALIZED POLYMERS

Номер: US20130224825A1
Автор: Bothof Catherine A., Fitzsimons, Griesgraber George W., Haddad Louis C., He Yi, Hembre James I., JR. Robert T., Rasmussen Jerald K., Satterwhite Erin A., Seshadri Kannan
Принадлежит: 3M INNOVATIVE PROPERTIES COMPANY

Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed. 1. A flocculant complex comprising a water soluble or water dispersible aminopolymer functionalized with guanidinyl groups , and target biological species selected from biomacromolecules and microbiological species.3. The flocculant complex of wherein said biomacromolecules are selected from proteins claim 1 , enzymes claim 1 , nucleic acids claim 1 , and endotoxins.4. The flocculant complex of wherein said biological species is selected from bacteria claim 1 , viruses claim 1 , cells claim 1 , cell debris claim 1 , and spores.5. The flocculant complex of wherein the amino polymer is selected from the group consisting of polyethylenimine claim 1 , polylysine claim 1 , polyaminoamides claim 1 , polyallylamine claim 1 , polyvinylamine claim 1 , polydimethylamine-epichlorohydrin-ethylenediamine claim 1 , polyaminosiloxanes and dendrimers formed from polyamidoamine (PAMAM) and polypropylenimine.6. The flocculant complex of wherein 0.1 to 100 mole percent of the available amino groups of the aminopolymer are functionalized with guanidinyl groups.7. The flocculant complex of wherein the guanidinyl groups of the functionalized aminopolymer are pendent from the polymer chain.8. The flocculant complex of wherein guanidinyl groups of the functionalized aminopolymer are in the aminopolymer chain.9. The flocculant complex of wherein the cells are selected from archaea claim 4 , bacteria claim 4 , and eucaryota.10. The flocculant complex of wherein the biological species is derived from a cell culture or fermentation process.11. The flocculant complex of wherein the amount of ligand-functionalized polymer relative to the amount of target biological species is 0.01% to 100% by weight.12. The flocculant complex of wherein a portion of the amino groups of the ligand-functionalized polymer further comprise alkyl or acyl groups.13. The ...

Подробнее
Номер записи: 29
29-08-2013 дата публикации

BIOACTIVE COMPOSITION INCLUDING STABILIZED PROTEIN AND PROCESS FOR PRODUCING THE SAME

Номер: US20130224826A1
Автор: Ishii Masahiko, Jia Hongfei, Wang Ping, Wu Songtao, Zhang Minjuan
Принадлежит:

A bioactive composition includes a porous hydrogel matrix. At least one protein is immobilized in the porous hydrogel matrix forming a hydrogel protein composite that is stable in an organic solvent. A process for stabilizing a bioactive composition includes the steps of: forming hydrogel matrix pores around protein molecules and reducing water content within the hydrogel matrix pores forming a hydrogel protein composite that is stable in an organic solvent. 1. A process for stabilizing a bioactive composition comprising:forming hydrogel matrix pores around protein molecules;reducing a water content within the hydrogel matrix pores forming a hydrogel-protein composite;and stabilizing activity of said protein molecules against inactivation by an organic solvent by said step of forming and said step of reducing.2. The process of wherein the step of forming hydrogel matrix pores around protein molecules includes the steps of dissolving the protein in deionized water at a desired concentration claim 1 , dissolving a prepolymer in deionized water at a desired concentration claim 1 , mixing the dissolved protein and dissolved prepolymer in a desired ratio claim 1 , and initiating a polymerization of the prepolymer.3. The process of wherein the step of initiating the polymerization includes at least one step selected from: adding a cross linking agent claim 2 , adding an initiator claim 2 , and adjusting a temperature of the mixture of the dissolved protein and dissolved prepolymer.4. The process of wherein the ratio of dissolved prepolymer is from 20-28 percent by weight in relation to the total volume.5. The process of wherein the step of reducing a water content within the hydrogel matrix pores comprises heating the hydrogel matrix to a temperature of from 20 to 110 degrees Celsius for a time period of from 24 hours to seven days.6. The process of wherein the step of reducing a water content within the hydrogel matrix pores comprises heating the hydrogel matrix to a ...

Подробнее
Номер записи: 30
05-09-2013 дата публикации

Covalently immobilized protein gradients in three-dimensional porous scaffolds

Номер: US20130230491A1
Автор: Charu Vepari, David L. Kaplan, Gordana Vunjak-Novakovic
Принадлежит: Massachusetts Institute of Technology, TUFTS UNIVERSITY

The invention provides a method for forming an immobilized agent gradient within a 3-dimensional porous scaffold. A 3-dimensional scaffold formed from a biocompatible material is provided. The surface of the scaffold and/or the agent is activated so as to allow binding of the agent to the scaffold. The activated scaffold is contacted with a solution containing the agent. Contact with the solution is maintained for a sufficient period of time to allow diffusion of the solution through a portion of the scaffold, thereby forming a desired gradient of the agent through the 3-dimensional scaffold.

Подробнее
Номер записи: 31
05-09-2013 дата публикации

Covalently Immobilized Enzyme and Method To Make The Same

Номер: US20130230896A1
Автор: Merchant Stephen A.
Принадлежит: Fresenius Medical Care Holdings, Inc.

A composition of enzyme, polymer, and crosslinker forms a network of covalently bound macromolecules. The covalently immobilized enzyme preparation has enzymatic activity, and retains stable activity when dried and stored at ambient conditions. Methods for preparing an immobilized enzyme and methods for using the enzyme are disclosed.

Подробнее
Номер записи: 32
19-09-2013 дата публикации

Dynamic Thermoresponsive Nanoparticles For Stabilization Of Enzymes At High Temperatures

Номер: US20130244301A1
Автор: Leech Anna M., Walker Jeremy P.
Принадлежит:

The present invention provides a thermoresponsive nanoparticle useful for the stabilization of enzymes in environments having a temperature greater than thirty degrees Centigrade. The thermoresponsive nanoparticle has (a) a functionalized enzyme conjugate having one or more enzymes or biological catalysts, the enzymes or biological catalysts are modified with palmitic acid N-hydroxysuccinimide ester and acryclic acid N-hydroxysuccinimide ester, and (b) a thermally responsive polymer, wherein the functionalized enzyme conjugate is encapsulated within the thermally responsive polymer. A nanocatalyst is provided that has one or more proteins. The proteins are covalently immobilized and encapsulated within a thermally responsive polymer shell. The proteins are one or more enzymes or biological catalysts. A method for protecting the proteins is also set forth. 1. A method for protecting proteins from environmental conditions comprising: immobilizing one or more proteins and encapsulating said one or more immobilized proteins within one or more polymeric nanoparticles for protecting said protein from detrimental environmental conditions.2. The method of including wherein said protein is at least one enzyme or at least one biological catalyst.3. The method of including covalently grafting at least one moiety to said enzyme for enabling said enzyme to covalently bind to said polymer.4. The method of including attaching a vinyl group as said moiety onto the surface of the enzyme via said covalent grafting.5. The method of including subjecting said graft-modified enzyme to a polymerization process.6. The method of including employing a free-radical addition polymerization process or a living radical polymerization process.7. The method of including wherein said free-radical polymerization process is selected from the group consisting of an emulsion polymerization process and a non-emulsion polymerization process.8. The method of including wherein said free-radical ...

Подробнее
Номер записи: 33
10-10-2013 дата публикации

POLYSILICATE-POLYSILICONE ENZYME IMMOBILIZATION MATERIALS

Номер: US20130267004A1
Автор: Bucholz Tracy L., Duesing Caroline M.H., Linder Alexander J., Powell Dawn C., Rambo Brett M., Weber Luke E., Zaks Aleksey
Принадлежит: AKERMIN, INC.

The present invention generally relates to improvements in enzyme immobilization, particularly for use in the field of carbon dioxide capture and sequestering. It has been discovered that the utilization of sol-gel processes to immobilize enzymes in polysilicate-polysilicone copolymer coatings and particles, and the deposition of these coatings on solid state supports or use of suspensions of these particles, provides significant benefits for use in industrial applications involving enzymatic catalysts. 13-. (canceled)4. A coated support comprising a polysilicate-polysilicone copolymer immobilizing a biocatalyst; the polysilicate-polysilicone copolymer adhered to a solid support by an adhesive coating and wherein the biocatalyst comprises a carbonic anhydrase.56-. (canceled)7. A coated support comprisinga solid support;a coating composition forming a layer on the surface of the solid support, the coating composition comprising a polysilicate-polysilicone copolymer and a hydrophilic additive; anda biocatalyst that catalyzes hydration of carbon dioxide being entrapped in the coating composition;wherein the biocatalyst comprises a carbonic anhydrase.8. (canceled)9. The coated support of wherein the adhesive coating comprises a polymer adhesive.10. The coated support of wherein the polymer adhesive comprises a urethane polymer claim 9 , an epoxy polymer claim 9 , a resin claim 9 , a cyanoacrylate polymer claim 9 , a methacrylate polymer claim 9 , or a combination thereof.1112-. (canceled)13. The coated support of wherein the polymer adhesive comprises a two-part epoxy polymer.14. (canceled)15. The coated support of wherein the coating composition is derived from reaction of a sol claim 7 , the sol comprising (i) an alkoxy silane or an organotrialkoxy silane or metasilicate claim 7 , (ii) a poly(silicone) claim 7 , (iii) a hydrophilic additive claim 7 , and (iv) the carbonic anhydrase.16. The coated support of wherein the coating composition is derived from reaction of ...

Подробнее
Номер записи: 34
07-11-2013 дата публикации

FIBROUS SUBSTRATES FOR CELL PROPAGATION AND DIFFERENTIATION

Номер: US20130295637A1
Автор: Lu Hongfang, Narayanan Karthikeyan, Wan Andrew Chwee Aun, Ying Jackie Y.
Принадлежит:

The present invention relates to a method of releasably encapsulating pluripotent embryonic stem cells in a degradable continuous polyionic fiber for tissue culture, wherein the encapsulated embryonic stem cells are able to maintain a pluripotent phenotype in tissue culture; the method comprising (a) contacting an aqueous solution of a polyanion with an aqueous solution of a polycation to form an interface between the aqueous solution of polyanion and the aqueous solution of polycation, and wherein the aqueous solution of polyanion or the aqueous solution of polycation or both the aqueous solution of polyanion and the aqueous solution of polycation comprises a suspension of pluripotent embryonic stem cells; (b) drawing a continuous polyionic fiber which comprises encapsulated pluripotent embryonic stem cells from the interface; (c) passing the continuous polyionic fiber comprising encapsulated pluripotent embryonic stem cells in a continuous process through a solution which reduces secondary complexation of the components of the polyionic fiber. 1. A method of releasably encapsulating pluripotent embryonic stem cells in a degradable continuous polyionic fiber for tissue culture , wherein the encapsulated embryonic stem cells are able to maintain a pluripotent phenotype in culture; the method comprising:(a) contacting an aqueous solution of a polyanion with an aqueous solution of a polycation to form an interface between the aqueous solution of polyanion and the aqueous solution of polycation, and wherein the aqueous solution of polyanion or the aqueous solution of polycation or both the aqueous solution of polyanion and the aqueous solution of polycation comprises a suspension of pluripotent embryonic stem cells;(b) drawing a continuous polyionic fiber which comprises encapsulated pluripotent embryonic stem cells from the interface; and(c) passing the continuous polyionic fiber comprising encapsulated pluripotent embryonic stem cells in a continuous process through ...

Подробнее
Номер записи: 35
14-11-2013 дата публикации

NOVEL USE OF LIPOLYTIC ENZYME FOR FORMATION OF ANTI-FINGERPRINT COATING, METHOD OF FORMING ANTI-FINGERPRINT COATING, SUBSTRATE COMPRISING THE ANTI-FINGERPRINT COATING FORMED BY THE METHOD, AND PRODUCT COMPRISING THE SUBSTRATE

Номер: US20130302875A1
Автор: Hong Young Jun, LEE Eun Jeong
Принадлежит:

Provided are a novel use of a lipolytic enzyme for forming anti-fingerprint coating, a method of forming anti-fingerprint coating including treating a substrate with a composition comprising the lipolytic enzyme, a substrate including the anti-fingerprint coating formed by the same method, and a product including the same. The anti-fingerprint coating can reduce contamination of display devices, appearances of electronic devices or building materials by fingerpris. 1. A method of forming anti-fingerprint coating , comprising treating a substrate with a composition comprising a lipolytic enzyme.2. The method according to claim 1 , wherein the lipolytic enzyme is a lipase.3. The method according to claim 2 , wherein the composition further includes at least one enzyme selected from the group consisting of a protease claim 2 , an amylase claim 2 , a cellulase claim 2 , and a lactase.4. The method according to claim 1 , wherein the substrate includes plastic or glass.5. The method according to claim 4 , wherein the plastic includes at least one polymer selected from the group consisting of polyester claim 4 , polypropylene claim 4 , polyethyleneterephthalate claim 4 , polyethylenenaphthalate claim 4 , polycarbonate claim 4 , triacetylcellulose claim 4 , olefin copolymers claim 4 , and polymethylmethacrylate.6. The method according to claim 1 , wherein the lipolytic enzyme is introduced to a surface of the substrate by adsorption claim 1 , covalent bonds claim 1 , or encapsulation.7. The method according to claim 6 , wherein the covalent bond is formed through a process including treating the surface of the substrate having at least one functional group selected from the group consisting of amino claim 6 , amide claim 6 , carboxyl claim 6 , aldehyde claim 6 , hydroxyl and thiol groups with a solution including a bifunctional cross-linker; and dipping the substrate in a buffer including the lipolytic enzyme.8. The method according to claim 6 , wherein the covalent bond is ...

Подробнее
Номер записи: 36
28-11-2013 дата публикации

Immobilized organophosphate-degrading enzymes and methods of making the same

Номер: US20130316431A1
Автор: Augustine A. Dinovo, David A. Schofield, Dominic P. Dinovo, Francis H. Verhoff, Matthew F. SMIECHOWSKI
Принадлежит: Individual

The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked organophosphate-degrading enzyme having a support material which includes a biomass material and/or a polymeric material. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized organophosphate hydrolase enzyme and enzyme materials.

Подробнее
Номер записи: 37
05-12-2013 дата публикации

Microbial Fermentation Methods and Compositions

Номер: US20130324407A1
Автор: Bogosian Gregg
Принадлежит:

The present invention provides methods for the cultivation of the genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture. 1MethylobacteriumMethylobacteriumMethylobacteriumMethylobacteriumMethylobacterium. A method for obtaining a preparation comprising growing a mono-culture or co-culture of in media that comprises a liquid phase and a solid phase , wherein said solid phase provides for increased yield of said relative to yield obtained by growing the in liquid media alone , thereby obtaining a preparation.2Methylobacterium. The method of claim 1 , further comprising harvesting grown in the media.3. The method of claim 1 , wherein at least substantially all of the solid phase is suspended in the liquid phase claim 1 , or wherein at least substantially all of the solid phase is not suspended in the liquid phase claim 1 , or wherein portions of the solid phase are suspended in the liquid phase and portions of the solid phase are not suspended in the liquid phase.46.-. (canceled)7MethylobacteriumMethylobacterium.. The method of claim 1 , wherein said solid phase provides for adherent growth of said and/or wherein said solid phase does not serve as a carbon source for said8. The method of claim 1 , wherein said solid phase comprises a solid substance selected from the group consisting of a man-made material claim 1 , a material of animal origin claim 1 , a material of plant origin claim 1 , a material of microbial origin claim 1 , a material of fungal origin claim 1 , a material of mineral origin claim 1 , and combinations thereof.9. The method of claim 8 , wherein said solid substance is inanimate.1012.-. (canceled)13. The method of claim 3 , wherein either: (i) the solid phase comprises at least about 0.02% to about 0.5% of said media and substantially all of the solid phase is ...

Подробнее
Номер записи: 38
12-12-2013 дата публикации

Methods For Fermentation of Xylose and Hexose Sugars

Номер: US20130330800A1
Автор: Rao Kripa, Relue Patricia Ann, Varanasi Sasidhar, Yuan Dawei
Принадлежит: The University of Toledo

Methods and systems for the isomerization and fermentation of xylose and hexose sugars using an immobilized enzyme system capable of sustaining two different pH microenvironments in a single vessel are disclosed. Bilayer particles are dispersed in a mixture comprising an ionic borate source and xylose. The bilayer particles have a first region with a first enzymatic activity comprising xylose isomerase and a pH of 6 or above, and a second region having a second enzymatic activity at an acidic pH. 1. A packed bed system comprising co-immobilized enzyme particles connected in series to a porous hollow fiber membrane fermentor , the co-immobilized enzyme particles comprising a first region having a first enzymatic activity and a second region having a second enzymatic activity.2. The system of claim 1 , further comprising a fermentation beer substantially free from yeast claim 1 , wherein the fermentation beer is capable of being concentrated for ethanol recovery.3. The system of claim 1 , comprising a recyclable aqueous solution containing buffers and borate.4. The system of claim 1 , comprising isomerization catalyst particles confined to a packed bed claim 1 , wherein the particles do not come into direct contact with yeast.5. The system of claim 1 , comprising a high density of yeast in the fermentor.6. The system of claim 5 , wherein the yeast is reusable following fermentation.7. The system of claim 1 , further comprising a batch vessel containing a mixture of glucose and xylose.8. The system of claim 7 , further comprising:a column having a flow channel, the column containing a desired quantity of the co-immobilized enzyme pellets;wherein the batch vessel, the column, and the fermentor are connected in a closed loop system.9. The system of claim 7 , wherein isomerization of xylose into xylulose occurs when the mixture comes into contact with the co-immobilized enzyme pellets.10. The system of claim 1 , wherein the co-immobilized enzyme pellets comprise urease ...

Подробнее
Номер записи: 39
26-12-2013 дата публикации

Plant derived cell culture material

Номер: US20130344036A1
Автор: Antti Laukkanen, Madhushree Bhattacharya, Marjo Yliperttula, Patrick Laurén, Yanru Lou
Принадлежит: UPM Kymmene Oy

The present invention relates material that is useful in culturing and transferring cells as well as delivering cells. The material comprises plant derived cellulose nanofibers or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.

Подробнее
Номер записи: 40
26-12-2013 дата публикации

Immortal Unipotent Porcine PICM-19H and PICM-19B Stem Cell Lines

Номер: US20130344154A1
Автор: Caperna Thomas J, Talbot Neil C., Willard Ryan R.
Принадлежит:

Two cell lines, PICM-19H and PICM-19B, were derived from the bipotent ARS-PICM-19 pig liver stem cell line. The unipotent porcine stem cell line PICM-19H differentiates exclusively into hepatocytes and can be induced to express CYP450 enzymes. The growth rate and cell density in culture, morphological features, and hepatocyte detoxification functions, i.e., inducible CYP450 activity, ammonia clearance, and urea production of the PICM-19H cells were evaluated for their application in artificial liver devices. PICM-19H cells contain numerous mitochondria, Golgi apparatus, smooth and rough endoplasmic reticulum, vesicular bodies and occasional lipid vacuoles and display inducible CYP450 activity, clear ammonia, and produce urea in a glutamine-free medium. The data indicate that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. The results demonstrate the potential for the use of PICM-19H cells in drug biotransformation and toxicity testing. 13-. (canceled)4. An isolated , immortal , unipotent porcine stem cell line wherein cells of said cell line are capable of differentiating exclusively into functional bile duct cells expressing cholangiocyte functions.5. The stem cell line of wherein said cells exhibit all the identifying characteristics of PICM-19B cells and said characteristics are that the cells are basolaterally polarized cells exhibiting basal membrane fluid transport claim 4 , high GGT activity and a lack of serum protein production.6. The stem cell line of wherein the stem cell line is the immortal claim 26 , unipotent PICM-19B stem cell line deposited as ATCC PTA-9173.7. The cell line according to wherein a culture of said cells further comprises feeder cells.8. A composition comprising cells of the cell line according to claim 4 , said cells being attached to microbeads.9. A composition comprising cells of the cell line according to claim 4 , said cells being encapsulated in alginate ...

Подробнее
Номер записи: 41
26-12-2013 дата публикации

Processing biomass

Номер: US20130344586A1
Автор: Harrison Medoff, Marshall Medoff, Thomas Craig Masterman
Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation.

Подробнее
Номер записи: 42
02-01-2014 дата публикации

Cell attachment coatings and methods using phosphorous-containing photoreagent

Номер: US20140004158A1
Автор: Joseph S. McGonigle
Принадлежит: Surmodics Inc

Cell attachment coatings for articles such as implantable medical devices and cell culture vessels are disclosed. The coatings include an intermediate coater layer which includes a phosphorous-containing component that is bonded in the coating by reacted photoreactive functional groups. The coating also include a second coated layer including an immobilized ECM protein or peptide that includes an active portion of an ECM protein that is able to serve as an outer layer to contact cells during use. The coatings promoted enhanced cell binding and growth.

Подробнее
Номер записи: 43
16-01-2014 дата публикации

Formation Of Droplet Or Hydrogel Arrays Using Hydrophilic-Hydrophobic Patterned Surfaces For High-Throughput Screening Applications

Номер: US20140018263A1
Автор: Boles Erica, Geyer Florian, Levkin Pavel
Принадлежит:

The present invention relates to a method of forming an array of separated homogenous fluid microdroplets or hydrogel micropads of a desired shape and size in a desired spatial pattern, comprising the steps of providing a patterned substrate with a solid support coated with a porous polymer layer or film comprising hydrophilic areas having the desired shape and size, surrounded by hydrophobic areas separating the hydrophilic areas into the desired spatial pattern, and applying the fluid to a multitude of the hydrophilic areas at the same time. 1. A method of forming an array of separated homogenous aqueous fluid microdroplets of a desired shape and size in a desired spatial pattern , comprising the steps of: (i) hydrophilic areas having the desired shape and size; surrounded by', '(ii) hydrophobic areas separating the hydrophilic areas into the desired spatial pattern;', 'and, '(a) providing a patterned substrate with a solid support coated with a porous polymer layer or film comprising'}(b) applying the aqueous fluid to a multitude of the hydrophilic areas at the same time.2. The method of claim 1 , wherein step (b) is performed manually.3. The method of claim 1 , wherein step (b) comprises submerging the patterned substrate in the aqueous fluid.4. The method of claim 1 , wherein step (b) comprises spreading an amount of the aqueous fluid along the surface of the patterned substrate.5. The method of claim 1 , wherein the aqueous fluid is selected from the group consisting of water claim 1 , aqueous solutions claim 1 , aqueous media claim 1 , and solutions that can form a hydrogel upon crosslinking or UV-initiated polymerization.6. The method of claim 1 , wherein the aqueous fluid contains biological specimens.7. The method of claim 6 , wherein the biological specimens are selected from the group consisting of cells claim 6 , bacteria claim 6 , viruses and multicellular organisms.8Danio rerioCaenorhabditis elegans.. The method of claim 7 , wherein the multicellular ...

Подробнее
Номер записи: 44
13-02-2014 дата публикации

Micellar polysiloxane enzyme immobilization materials

Номер: US20140045241A1
Автор: Tizah ANJEH, Tracy BUCHOLZ
Принадлежит: AKERMIN Inc

The present invention generally relates to immobilized enzymes for use in carbon capture and other systems; particularly, materials used to immobilize carbonic anhydrase are disclosed.

Подробнее
Номер записи: 45
20-02-2014 дата публикации

SILICA ENCAPSULATED BIOMATERIALS

Номер: US20140051144A1
Автор: Aksan Alptekin, Kasinkas Lisa, Reategui Eduardo, Sadowsky Michael J, Wackett Lawrence P.
Принадлежит: Regents of the University of Minnesota

The present invention relates to compositions for encapsulation of biomaterials in a silica-matrix. The present invention includes a composition for formation of a silica-matrix encapsulated biomaterial. The composition includes a reactive silicon compound and a biomaterial with a catalytic activity. When encapsulated in the silica-matrix, the biomaterial at least partially retains its catalytic activity. The present invention also relates to methods of making silica-matrix encapsulated biomaterials, and to methods of using silica-matrix encapsulated biomaterials, including methods of treating water or gas using the silica-matrix encapsulated biomaterials. 1. A composition for formation of a silica-matrix encapsulated biomaterial , comprising:a reactive silicon compound; and,a biomaterial with a catalytic ability;wherein the silica-encapsulated biomaterial at least partially retains its catalytic ability.2. The composition of claim 1 , wherein the catalytic ability of the silica-encapsulated biomaterial comprises conversion of atrazine into a different compound claim 1 , conversion of a fracking chemical to a less toxic compound claim 1 , or conversion of a gas to a less flammable claim 1 , less explosive claim 1 , or less toxic compound.3. The composition of claim 1 , wherein the biomaterial comprises at least one of a bacteria claim 1 , archaea claim 1 , protist claim 1 , fungi claim 1 , or enzyme.4. The composition of claim 1 , wherein enzyme causes at least part of the catalytic activity claim 1 , or wherein the biomaterial expresses at least one enzyme that causes the at least part of the catalytic activity.5. The composition of claim 4 , wherein the biomaterial expresses atrazine chlorohydrolase (AtzA).6. The composition of claim 1 , wherein the reactive silicon compound comprises a silanol.7. The composition of claim 1 , further comprising water.8. The composition of claim 1 , further comprising an organic precursor.9. The composition of claim 8 , wherein the ...

Подробнее
Номер записи: 46
20-03-2014 дата публикации

NANOSTRUCTURED CARBON BASED BIOCATALYST FOR REMEDIATION OF ENVIRONMENTAL POLLUTANTS

Номер: US20140080197A1
Автор: Lin Chung-Ho, Thompson Brian
Принадлежит: The Curators of the University of Missouri

The present invention provides a series of new and improved compounds/materials as vehicles to delivery degradative enzymes to remove/remedy environmental pollutants. The inventive material comprises a series of amide-functionalized ordered mesoporous carbon (AFOMC), which utilizes chemical conjugation techniques for the tethering of enzymes to the surface of the synthesized AFOMC. The delivery mechanism may be utilized to express a wide variety of toxin-degrading enzymes for removal/remediation of organic pollutants. 1. A method of reducing pollutants and/or toxins in an environment comprising the steps of introducing to a contaminated environment an amide-functionalized ordered mesoporus carbon , wherein the amide-functionalized ordered mesoporus carbon has at least one toxin-degrading enzyme conjugated to the surface thereof.2. The method of claim 1 , wherein at least 20% of the pollutants and/or toxins are removed from the environment.3. The method of claim 1 , wherein at least 50% of the pollutants and/or toxins are removed from the environment.4. The method of claim 1 , wherein the contaminated environment is selected from the group consisting of liquid claim 1 , solid claim 1 , semi-solid claim 1 , and gaseous environments.5. The method of claim 1 , wherein the contaminated environment is contaminated with a contaminate selected from the group consisting of atrazine claim 1 , ortho-Nitrophenly-β-galactosidase claim 1 , and combinations thereof.6. The method of claim 9 , wherein the contaminate is atrazine.7. The method of claim 1 , wherein the toxin-degrading enzyme reacts with the contaminate whereby reducing the amount of the contaminant found in the contaminated environment.8. A biofilter comprising a porous material and an amide-functionalized ordered mesoporus carbon claim 1 , wherein the amide-functionalized ordered mesoporus carbon has at least one toxin-degrading enzyme conjugated to the surface thereof.911. The biofilter of claim claim 1 , wherein ...

Подробнее
Номер записи: 47
03-04-2014 дата публикации

CELL-ADHESIVE PROTEIN

Номер: US20140094590A1
Автор: Ogiwara Kazutaka
Принадлежит: FUJIFILM Corporation

It is an object of the present invention to provide a protein having high cellular adhesiveness that is useful as a cell adhesion support. The present invention provides a cell-adhesive protein comprising methionine, wherein at least a portion of the methionine residues is oxidized. 1. A cell-adhesive protein comprising methionine , wherein at least a portion of methionine residues is oxidized.2. The cell-adhesive protein according to claim 1 , wherein 7% or more of the methionine residues in the protein is oxidized.3. The cell-adhesive protein according to claim 1 , wherein at least a portion of the methionine residues is oxidized by an oxidizing agent.4. The cell-adhesive protein according to claim 1 , the cellular adhesiveness of which is improved by oxidization of the methionine residue.5. The cell-adhesive protein claim 1 , according to claim 1 , which is a gelatin-like protein.6. The cell-adhesive protein according to claim 5 , wherein the gelatin-like protein is gelatin claim 5 , collagen claim 5 , fibronectin claim 5 , pronectin claim 5 , vitronectin claim 5 , or a combination thereof.7. The cell-adhesive protein according to claim 5 , wherein the gelatin-like protein is a recombinant gelatin having an amino acid sequence derived from a partial amino acid sequence of collagen.8. The cell-adhesive protein according to claim 7 , wherein the recombinant gelatin has repeats of a sequence represented by Gly-X-Y (wherein X and Y each independently represent any amino acid) that is characteristic to collagen (wherein a plurality of sequences Gly-X-Y may be identical to or different from one another) claim 7 , and has a molecular weight of 2 KDa or more and 100 KDa or less.9. The cell-adhesive protein according to claim 7 , wherein the recombinant gelatin has repeats of a sequence represented by Gly-X-Y (wherein X and Y each independently represent any amine acid) that is characteristic to collagen (wherein a plurality of sequences Gly-X-Y may be identical to or ...

Подробнее
Номер записи: 48
10-04-2014 дата публикации

CELL-ADHERING LIGHT-CONTROLLABLE SUBSTRATE

Номер: US20140099695A1
Автор: FURUTA Toshiaki, Ozawa Satoshi, Sugiyama Hisashi, SUZUKI Akinobu, TADA Hiroko
Принадлежит:

An object of the present invention is to enable simpler operation in real time and culture while removing unnecessary cells from cultured cells for purification in analyzing, fractionating, and culturing the cells alive and to analyze and fractionate desired cells from the cultured cells to increase the purity, recovery rate, and viability of the cells. The present invention employs a cell-adhesive photocontrollable base material, wherein light irradiation causes the bond dissociation of a photolabile group comprising a coumarinylmethyl skeleton to produce the separation of a cell-adhesive material to leave a non-cell-adhesive material. As a result, cell images can be detected and analyzed to obtain the positional information of desired cells. Based on the positional information thus obtained, the cells can be analyzed and fractionated alive. 1. A cell-adhesive photocontrollable base material , wherein light irradiation causes the bond dissociation of a photolabile group comprising a coumarinylmethyl skeleton to separate a cell-adhesive material and leave a non-cell-adhesive material on a base material.2. A cell-adhesive photocontrollable base material obtained by conducting a film formation of a cell-adhesive photocontrollable material on the base material , wherein a cell-adhesive material binds to a non-cell-adhesive material via a photolabile group comprising a coumarinylmethyl skeleton in the cell-adhesive photocontrollable material in which the non-cell-adhesive material , the photolabile group and the cell-adhesive material are bound in this order from the base material side. to3. A cell-adhesive photocontrollable base material , wherein light irradiation causes the bond dissociation of a photolabile group comprising a coumarinylmethyl skeleton to irreversibly change the surface of the irradiated portion from the cell-adhesive material to the non-cell-adhesive material.12. The cell-adhesive photocontrollable base material according to claim 1 , wherein the ...

Подробнее
Номер записи: 49
06-01-2022 дата публикации

CELL CULTURE CARRIER, AND METHOD AND DEVICE FOR PRODUCING SAME

Номер: US20220002704A1
Автор: Aratani Tomoyuki, Iwashita Natsuko, Sameshima Tatsuya, Satoh Naoki, Shionoiri Momoko, Yaginuma Hidekazu, Yamazaki Takehiro
Принадлежит: RICOH COMPANY, LTD.

This invention is to develop a production method for a cell culture carrier comprising a hydrogel having high shape retainability, and cell culture carrier production device, and to provide a structure composed of a cell culture carrier produced using the same. 1. A production method for a cell culture carrier comprising:a retention step of retaining a first solution containing a multiple branching polymer with one or more nucleophilic functional groups or electrophilic functional groups at a side chain(s) and/or a terminal(s), comprising polyethylene glycol as the backbone, anda gel formation step of forming one or more hydrogels by landing a droplet of a second solution discharged from a droplet discharge device in the first solution so that the droplet contacts with the retained first solution,wherein the second solution contains a multiple branching polymer with one or more other nucleophilic functional groups or electrophilic functional groups at a side chain(s) and/or a terminal(s), comprising polyethylene glycol as the backbone.2. The production method for a cell culture carrier according to claim 1 , wherein the first solution is retained on a support material.3. The production method for a cell culture carrier according to or claim 1 , wherein the volume of the droplet is 9 μL or more and 900 μL or less.4. The production method for a cell culture carrier according to any one of to claim 1 , wherein the volume of the hydrogel formed per one landing is 9 μL or more and 900 μL or less.5. The production method for a cell culture carrier according to any one of to claim 1 , wherein all or part of the hydrogels are in contact with each other.6. The production method for a cell culture carrier according to claim 5 , wherein the hydrogels constitute a three-dimensional structure stacked so that all or part of two or more hydrogels overlap each other.7. The production method for a cell culture carrier according to any one of to comprising a removal step of removing ...

Подробнее
Номер записи: 50
02-01-2020 дата публикации

BONE AUGMENTATION UTILIZING MUSCLE-DERIVED PROGENITOR COMPOSITIONS IN BIOCOMPATIBLE MATRIX, AND TREATMENTS THEREOF

Номер: US20200000966A1
Автор: Huard Johnny, Jankowski Ronald, Payne Karin, Payne Thomas, Usas Arvydas
Принадлежит:

The present invention provides muscle-derived progenitor cells that show long-term survival following transplantation into body tissues and which can augment non-soft tissue following introduction (e.g. via injection, transplantation, or implantation) into a site of non-soft tissue (e.g. bone) when combined with a biocompatible matrix, preferably SIS. The invention further provides methods of using compositions comprising muscle-derived progenitor cells with a biocompatible matrix for the augmentation and bulking of mammalian, including human, bone tissues in the treatment of various functional conditions, including osteoporosis, Paget's Disease, osteogenesis imperfecta, bone fracture, osteomalacia, decrease in bone trabecular strength, decrease in bone cortical strength and decrease in bone density with old age. 134.-. (canceled)35. A method of treating a bone disease , defect or pathology in a human subject in need thereof , wherein the method comprises administering to the human subject small intestine submucosa (SIS) seeded with a cell population enriched for muscle derived progenitor cells (MDCs) , wherein the cell population enriched for MDCs is isolated from skeletal muscle by a method comprising:a. suspending skeletal muscle cells obtained from the human in a first cell culture container for between 30 and 120 minutes to produce a population of adherent cells and a population of non-adherent cells;b. decanting media and the population of non-adherent cells from the first cell culture container to a second cell culture container;c. allowing the population of decanted, non-adherent cells in the media to attach to the walls of the second cell culture container; andd. isolating the population of cells from the walls of the second cell culture container, wherein the isolated population of cells is the cell population enriched for MDCs.36. The method of claim 35 , wherein the isolation method further comprises culturing the cell population enriched for MDCs to ...

Подробнее
Номер записи: 51
04-01-2018 дата публикации

NANO-BIOREACTOR APPARATUS AND METHOD OF MANIPULATING EXTRACELLULAR METABOLIC SYSTEMS

Номер: US20180002171A1
Автор: Amoabediny Ghassem, Pesaran Mahdi
Принадлежит:

A nano-bioreactor, and an enzymatic nano-bioreactor apparatus are disclosed. The enzymatic nano-bioreactor, comprises at least one enzyme, and a cellulose nano-fiber. The enzyme is any one of a urease, a glutamate dehydrogenase, and a glutamine synthetase configured to immobilize on the cellulose nano-fiber. The enzymatic nano-bioreactor apparatus comprises a plurality of input pumps, which is configured to introduce at least one input to a plurality of micro-bioreactors, where connected each other by a connector. The apparatus further comprises, at least one nano-bioreactor placed inside the micro-bioreactor, configured to receive the input, and an outlet configured to extrude a resultant output from the nano-bioreactor to a collector unit. The present also discloses a method for manipulating extracellular metabolic system using the said enzymatic nano-bioreactor apparatus. 1. An enzymatic nano-bioreactor , comprising:at least one enzyme, anda nano-fiber,wherein the enzyme is a nanoparticle configured to immobilize on the nano-fiber.2. The enzymatic nano-bioreactor of claim 1 , wherein the enzyme is any one of a urease claim 1 , a glutamate dehydrogenase claim 1 , and a glutamine synthase.3. The enzymatic nano-bioreactor of claim 1 , wherein the nano-fiber is a bacterial cellulose nano-fiber.4. The enzymatic nano-bioreactor of claim 1 , wherein the enzyme is immobilized by crosslinking mechanism to the nanofibers.5. The enzymatic nano-bioreactors of claim 1 , wherein the enzyme comprising urease nanoparticle configured to immobilize on the nano-fiber to form a urease nano-bioreactor.6. The enzymatic nano-bioreactors of claim 1 , wherein the enzyme comprising glutamate dehydrogenase nanoparticle is configured to immobilize on the nano-fiber to form a glutamate dehydrogenase nano-bioreactor.7. The enzymatic nano-bioreactors of claim 1 , wherein the enzyme comprising glutamine synthetase nanoparticle configured to immobilize on the nano-fiber to form a glutamine ...

Подробнее
Номер записи: 52
07-01-2016 дата публикации

Method for preparing a hydrogel matrix by photopolymerization

Номер: US20160002368A1
Автор: Abbas Mgharbel, Alice NICOLAS, Danielle Gulino
Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS, Commissariat a lEnergie Atomique et aux Energies Alternatives CEA

The invention relates to a method for preparing a hydrogel type matrix comprising at least two contiguous zones with distinct stiffnesses, comprising a step to photopolymerize a solution comprising one or several polymerizable compounds and a photopolymerization initiator, by application of light onto the entire solution and at a different intensity in at least two zones of the solution, as a result of which a hydrogel matrix is obtained comprising at least two contiguous zones with distinct stiffnesses, characterized in that the photopolymerization step is done in the presence of a polymerization catalyst.

Подробнее
Номер записи: 53
05-01-2017 дата публикации

METHOD OF ATTACHING A CELL-OF-INTEREST TO A MICROTUBE

Номер: US20170002343A1
Автор: GREEN Michal, KLEIN Shiri, KUHN Jonathan Charles, ZUSSMAN Eyal
Принадлежит:

A method of attaching a cell or a membrane-coated particle-of-interest to a microtube is provided. The method comprising: co-electrospinning two polymeric solutions through co-axial capillaries, wherein a first polymeric solution of the two polymeric solutions is for forming a shell of the microtube and a second polymeric solution of the two polymeric solutions is for forming a coat over an internal surface of the shell, the first polymeric solution is selected solidifying faster than the second polymeric solution and a solvent of the second polymeric solution is selected incapable of dissolving the first polymeric solution and wherein the second polymeric solution comprises the cell or the membrane-coated particle-of-interest, thereby attaching the cell or the membrane-coated particle-of-interest to the microtube. Also provided are microtubes with attached, entrapped or encapsulated cells or membrane-coated particles and methods of using same 1. A microtube comprising:an electrospun shell,an electrospun coat polymer over an internal surface of said shell and a cell or membrane-coated particle-of-interest attached to the microtube,wherein said electrospun shell is formed of a first polymeric solution comprising a first solvent and said electrospun coat is formed of a second polymeric solution comprising a second solvent,wherein said second solvent of said second polymeric solution is incapable of dissolving a polymer of said first polymeric solution,wherein said first polymeric solution solidifies faster than said second polymeric solution,wherein said second polymeric solution is capable of wetting said internal surface of said shell during or following solidification of said first polymeric solution.2. The microtube of claim 1 , wherein said polymer of said first polymeric solution and a polymer of said second polymeric solution are different.3. The microtube of claim 1 , wherein said electrospun shell comprises pores.4. The microtube of claim 1 , wherein said ...

Подробнее
Номер записи: 54
04-01-2018 дата публикации

IMMOBILIZED CELL AND PREPARATION METHOD THEREOF

Номер: US20180002686A1
Автор: Li Naiqiang, Liu Charlie, LIU Xiucai, Yu Lijun
Принадлежит:

Provided are a lysine decarboxylase immobilized cell and preparation method thereof. 2. The method of immobilizing cells containing lysine decarboxylase according to claim 1 , wherein the resulting solution further comprises one or more of a natural macromolecule material and a second synthetic macromolecule material claim 1 , or a mixture thereof claim 1 , wherein the natural macromolecule material and the second synthetic macromolecule material are natural or synthetic macromolecule material having a gel-forming ability claim 1 , and the second synthetic macromolecule material is different from the first synthetic macromolecule material.3. The method of immobilizing cells containing lysine decarboxylase according to claim 2 , wherein the first synthetic macromolecule material claim 2 , the second synthetic macromolecule material are one or more selected from the group consisting of polyvinyl alcohol claim 2 , polyethylene glycol claim 2 , polyacrylonitrile claim 2 , acetate fibers claim 2 , and diacetate fibers claim 2 , preferably selected from the group consisting of polyvinyl alcohol and polyethylene glycol.4. The method of immobilizing cells containing lysine decarboxylase according to claim 2 , wherein the natural macromolecule material is one or more selected from the group consisting of sodium alginate claim 2 , carrageenan claim 2 , xanthan gum claim 2 , activated carbon claim 2 , diatomaceous earth claim 2 , curdlan claim 2 , and chitosan claim 2 , preferably sodium alginate.5. The method of immobilizing cells containing lysine decarboxylase according to claim 2 , wherein the resulting solution is a mixed solution of polyvinyl alcohol and sodium alginate or a mixed solution of polyvinyl alcohol and polyethylene glycol claim 2 , preferably claim 2 , the addition amount of the polyvinyl alcohol is 1 to 13 wt % claim 2 , the addition amount of sodium alginate is 0.1 to 3 wt % claim 2 , and the addition amount of the polyethylene glycol is 0.1 to 5 wt %.6. ...

Подробнее
Номер записи: 55
02-01-2020 дата публикации

HIERARCHICAL MAGNETIC NANOPARTICLE-ENZYME MESOPOROUS ASSEMBLIES EMBEDDED IN MACROPOROUS SCAFFOLDS

Номер: US20200002698A1
Автор: ANESHANSLEY Daniel, CORGIE Stephane C., Duan Xiaonan, Giannelis Emmanuel, WALKER Larry P.
Принадлежит: CORNELL UNIVERSITY

A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion. 122.-. (canceled)23. A method for epoxidation reactions of alkenes , the method comprising reacting alkenes in the presence of oxygen with a hierarchical catalyst composition comprising a continuous macroporous scaffold in which is incorporated self-assembled mesoporous aggregates of magnetic nanoparticles containing an oxygen-transfer enzyme embedded in mesopores of said mesoporous aggregates of magnetic nanoparticles , to produce an alkene oxide.24. The method of claim 23 , wherein said oxygen-transfer enzyme is a chloroperoxidase or a lipase.2539.-. (canceled)40. The method of claim 23 , further comprising magnetic particles claim 23 , not belonging to said mesoporous aggregates of magnetic nanoparticles claim 23 , embedded in said continuous macroporous scaffold.41. The method of claim 23 , wherein said continuous macroporous scaffold has a polymeric composition.42 ...

Подробнее
Номер записи: 56
01-01-2015 дата публикации

PARTICLES COMPRISING A RELEASABLE DOPANT THEREIN

Номер: US20150004095A1
Автор: Barbe Christophe Jean Alexandre, Finnie Kim Suzanne, KONG Linggen
Принадлежит:

A process for making particles comprising a hydrophobic dopant for subsequent release therefrom is disclosed. The process comprises providing an emulsion comprising a hydrophilic phase and a hydrophobic phase dispersed in the hydrophilic phase, and reacting the precursor material to form the particles comprising the dopant therein. The hydrophobic phase comprises a precursor material and the dopant. 125.-. (canceled)26. A process for making particles comprising a hydrophobic dopant therein said dopant being releasable from the particles , the process comprising:forming an emulsion comprising a hydrophilic phase and a hydrophobic phase dispersed in the hydrophilic phase, said hydrophobic phase comprising a precursor material and the dopant;adding an aminofunctional silane catalyst to the emulsion, whereby the catalyst penetrates into the hydrophobic phase; andreacting the precursor material in the presence of the catalyst to form the particles comprising the dopant therein,wherein (i) each of the particles is a porous solid particle and (ii) the dopant is distributed substantially homogeneously within and is releasable by diffusion from each particle.27. The process of claim 26 , wherein said hydrophobic phase additionally comprises a solvent.28. The process of claim 26 , wherein said precursor material is selected from the group consisting of hydrolysable organosilica precursors claim 26 , organotitania precursors claim 26 , organoalumina precursors claim 26 , organozirconia precursors claim 26 , and a mixture of any two or more thereof.29. The process of claim 26 , wherein the precursor material comprises an organosilane.30. The process of claim 29 , wherein the organosilane is an organotrialkoxysilane.31. The process of claim 30 , wherein the organotrialkoxysilane is selected from the group consisting of ethyltrimethoxysilane claim 30 , vinyltrimethoxysilane claim 30 , phenyltrimethoxysilane claim 30 , isopropyltrimethoxysilane claim 30 , benzyltrimethoxysilane ...

Подробнее
Номер записи: 57
07-01-2021 дата публикации

SYSTEMS AND METHODS FOR GROWING A BIOFILM OF PROBIOTIC BACTERIA ON SOLID PARTICLES FOR COLONIZATION OF BACTERIA IN THE GUT

Номер: US20210002627A1
Автор: Baram David, DABOUSH David, DIAMANT Rachel
Принадлежит:

The present invention provides a method, wherein the method forms a biofilm, wherein the biofilm comprises a population of at least one bacterial strain attached to particles, wherein the biofilm is configured to colonize a gut of a subject in need thereof for at least five days, when ingested by the subject, the method comprising: a. obtaining a population comprising at least one strain of bacteria; b. inoculating a growth medium containing particles with the population comprising at least one strain of bacteria; c. incubating the particles with the population comprising at least one bacterial strain for a time sufficient for the population of at least one strain of bacteria to attach to the particles; and d. culturing the population comprising at least one strain of bacteria attached to the particles in a growth medium, for a time sufficient to form a biofilm. 2. The method of claim 1 , wherein the population of at least one strain of bacteria attached to the particles is first cultured in the growth medium under static conditions claim 1 , followed by culture in the growth medium under flow conditions.3. The method of claim 1 , wherein the particles are porous particles ranging from 30 to 500 microns in diameter.4. The method of claim 1 , wherein the particles are selected from the group consisting of: seeds claim 1 , dicalcium phosphate claim 1 , and cellulose.5. The method of claim 1 , wherein said particle is a porous particle.6. The method of claim 1 , wherein the population comprising at least one bacterial strain is selected from gut microflora.7. The method of claim 1 , wherein said biofilm is acid tolerant.8. The method of claim 1 , wherein said biofilm is configured for pH dependent targeted release of the bacterial biofilm in the gastrointestinal tract.9. The method of claim 1 , wherein the biofilm is encapsulated with a compound configured to release the at least one bacterial strain at a pH found in the intestine of an animal.10. The method of claim 9 ...

Подробнее
Номер записи: 58
07-01-2021 дата публикации

TARGET SUBSTANCE DETECTION METHOD, TARGET SUBSTANCE DETECTION KIT, AND TARGET SUBSTANCE DETECTION DEVICE

Номер: US20210002692A1
Автор: Abe Tomoteru, Hosokawa Hiroyuki
Принадлежит:

To provide a method for detecting a target substance by using an interaction through a molecular species having low reactivity, with two types of microparticles as proximity probes. A target substance detection method includes the following steps of: a step (A) of bringing a biological sample into contact with an oxygen sensor bound to a first molecule that specifically binds to a target substance; a step (B) of bringing the biological sample into contact with a first solid phase support that holds a second molecule and an oxygen-consuming enzyme, the second molecule specifically binding to the target substance; and a step (C) of acquiring information generated from the oxygen sensor by the steps (A) and (B). 1. A target substance detection method , comprising following steps of:a step (A) of bringing a biological sample into contact with an oxygen sensor bound to a first molecule that specifically binds to a target substance;a step (B) of bringing the biological sample into contact with a first solid phase support that holds a second molecule and an oxygen-consuming enzyme, the second molecule specifically binding to the target substance; anda step (C) of acquiring information generated from the oxygen sensor by the steps (A) and (B).2. The target substance detection method according to claim 1 , wherein in the step (A) claim 1 , the oxygen sensor is held on a second solid phase support.3. The target substance detection method according to claim 1 , wherein the biological sample contains living cells.4. The target substance detection method according to claim 3 , further comprising a step (D) of bringing the living cells into contact with a cell stimulating substance.5. The target substance detection method according to claim 4 , wherein the steps (A) to (D) are performed in a well enabled to capture a single cell.6. The target substance detection method according to claim 1 , wherein the target substance is selected from a group consisting of proteins claim 1 , ...

Подробнее
Номер записи: 59
03-01-2019 дата публикации

Dephosphorylated lysosomal storage disease proteins and methods of use thereof

Номер: US20190002852A1
Автор: Reinhard Gabathuler, Timothy Z. Vitalis
Принадлежит: Bioasis Technologies Inc

Provided are substantially dephosphorylated forms of lysosomal storage disease (LSD) proteins, including dephosphorylated forms of iduronate-2-sulfatase (IDS, or I2D) and iduronidase (IDU), having increased ability to traverse or penetrate the blood brain barrier (BBB) relative to phosphorylated forms of the protein, and p97 conjugates thereof. Also provided are compositions comprising such dephosphorylated LSD proteins and p97 conjugates, and methods of use thereof, for instance, to treat any one or more lysosomal storage diseases, such as Hunter Syndrome (or MPS Type II).

Подробнее
Номер записи: 60
13-01-2022 дата публикации

OPTIMIZED METHOD FOR PRODUCING A COMPOSITION CONTAINING ISOMALTULOSE

Номер: US20220010345A1
Автор: Rose Thomas, Wach Wolfgang
Принадлежит:

The present invention relates to a method for producing a composition containing isomaltulose from a substrate containing sucrose comprising the steps of: a) contacting the substrate containing sucrose with a particulate carrier-immobilized sucrose isomerase biomass and b) obtaining a composition containing isomaltulose, characterized in that the median particle size d(0.5) of the carrier-immobilized sucrose isomerase biomass is from 370 to 550 μm. The carrier can be an alginate or a polyvinyl alcohol carrier. 1. A method for producing a composition containing isomaltulose from a substrate containing sucrose , comprising the steps of:a) contacting the substrate containing sucrose with a particulate carrier-immobilized sucrose isomerase biomass, wherein the carrier is an alginate or polyvinyl alcohol carrier andb) obtaining a composition containing isomaltulose,characterized in that the median particle size d(0.5) of the carrier-immobilized sucrose isomerase biomass is from 400 to 500 μm,{'i': 'Protaminobacter rubrum.', 'wherein the sucrose isomerase biomass originates from'}2. The method according to claim 1 , wherein the particulate carrier-immobilized sucrose isomerase biomass has d(0.5) from 420 to 470 μm.3. The method according to claim 1 , wherein the particulate carrier-immobilized sucrose isomerase biomass is spherical.4. The method according to claim 1 , wherein the weight ratio of sucrose isomerase biomass to carrier is 10 to 6 parts sucrose isomerase biomass to 6 to 2 parts of the carrier (in each case dry weight).5. The method according to claim 1 , wherein the composition containing isomaltulose contains trehalulose.6. The method according to claim 1 , wherein the sucrose isomerase biomass is carrier-immobilized by bonding claim 1 , crosslinking or entrapment immobilization.7. The method according to claim 1 , wherein the sucrose isomerase biomass is a sucrose isomerase claim 1 , a microorganism cell with sucrose isomerase activity or a cell-extract with ...

Подробнее
Номер записи: 61
01-01-2015 дата публикации

MICROTHREAD DELIVERY SYSTEM

Номер: US20150005739A1
Автор: Cornwall Kevin, Gaudette Glenn, Guyette Jacques P., Pins George D., Rolle Marsha
Принадлежит:

Compositions that include microthreads are provided. The compositions can be fully or partially encased in a sleeve along at least a portion of their length and can include biological cells and, optionally, therapeutic agents. Also provided are methods for using the compositions to repair or ameliorate damaged or defective tissue, including cardiovascular tissue (e.g., the myocardium). 135-. (canceled)36. A method for treating a tissue , comprising:(a) drawing a fibrin thread removably coupled to a needle through a region of tissue in need of repair, the fibrin thread having a biological cell disposed thereon and(b) removing the needle from the fibrin thread so that the fibrin thread comprising the biological cell is retained in the tissue in need of repair,wherein the biological cell causes an improvement in the tissue.37. The method of claim 36 , wherein the biological cell is a stem cell.38. The method of claim 37 , wherein the stem cell is one or more of an undifferentiated stem cell and a partially differentiated cell.39. The method of claim 36 , wherein the biological cell is a differentiated cell.40. The method of claim 39 , wherein the differentiated cell is a myocyte.41. The method of claim 36 , wherein the biological cell comprises a plurality of stem cells and myocytes.42. The method of claim 36 , wherein the fibrin thread further comprises a therapeutic agent.43442. The method of claim claim 36 , wherein the therapeutic agent is one or more of a growth factor claim 36 , a protein claim 36 , a vitamin claim 36 , a mineral claim 36 , an antimicrobial agent claim 36 , or a small organic molecule.44. A method of making a tissue repair composition claim 36 , the method comprising:(a) culturing biological cells that induce or enhance regeneration of tissue in the presence of a fibrin thread under conditions that allow the biological cells to attach to the fibrin thread and(b) removably coupling the fibrin thread to a needle,wherein the fibrin thread with the ...

Подробнее
Номер записи: 62
14-01-2021 дата публикации

LACTASE-CONTAINING DOUBLE MICROCAPSULE, PREPARATION METHOD THEREFOR, AND USE THEREOF

Номер: US20210007367A1
Автор: AHN Sung-Il, KWAK Hae-Soo
Принадлежит:

The present invention provides a lactase-containing double microcapsule which includes; lactase provided as a core material; and a primary coating material and a secondary coating material, which are sequentially coated on the core material to be formulated, a method for preparing the lactase-containing double microcapsule, and a dairy product including the lactase-containing double microcapsule as a use of the lactase-containing double microcapsule. 1. A lactase-containing double microcapsule comprising: lactase provided as a core material; and a primary coating material and a secondary coating material , which are sequentially coated on the core material to be formulated.2. The lactase-containing double microcapsule according to claim 1 , wherein the primary coating material includes at least one selected from the group consisting of medium-chain triglyceride (MCT) claim 1 , hydrogenated corn oil claim 1 , soybean oil claim 1 , safflower seed oil and butter oil.3. The lactase-containing double microcapsule according to claim 1 , wherein the secondary coating material includes at least one selected from the group consisting of hydroxypropyl methylcellulose phthalate claim 1 , zein claim 1 , shellac claim 1 , Eudragit claim 1 , cellulose acetate phthalate claim 1 , cellulose acetate succinate claim 1 , polyvinyl acetate phthalate claim 1 , cellulose acetate trimellitate claim 1 , hypromellose acetate succinate and phenyl salicylate.4. The lactase-containing double microcapsule according to claim 1 , wherein the formulation is in any form selected from the group consisting of powders claim 1 , solution claim 1 , tablets claim 1 , and granules.5. A method for preparing lactase-containing double microcapsule comprising:providing lactase as a core material, and stirring the core material with a primary coating material and a primary emulsifier to obtain an emulsion of lactase;stirring the emulsion of lactase with secondary coating material and a secondary emulsifier ...

Подробнее
Номер записи: 63
10-01-2019 дата публикации

Bacterial inoculating formulation based on a microorganism consortium of genus calothrix sp. to increase yield and quality of vegetable crops, the method for manufacturing the formulation and uses thereof

Номер: US20190008161A1
Автор: Héctor Álan BARRÓN LEÓN, Ivan Alejandro De La Pena Mireles, Miguel Angel BAUTISTA RAMÍREZ, Salvador OCEGUEDA ESTRADA
Принадлежит: NEWPEK SA de CV

The bacterial inoculating formulation based on a microorganism consortium of genus calothrix sp., to increase yield and quality of vegetable crops comprises: a) a microorganism consortium of genus calothrix sp., at a concentration ranging from 0.05% to 10% by weight; b) a substrate or immobilizing vehicle in order to immobilize the microorganisms at a concentration ranging from 2% to 80% by weight; c) a soil pH buffer at a concentration ranging from 0% to 3% by weight, and d) moisture at a concentration ranging from 7% to 97.95% by weight.

Подробнее
Номер записи: 64
14-01-2016 дата публикации

MICROCAPSULES AND CONCRETE CONTAINING THE SAME

Номер: US20160009596A1
Автор: De Belie Nele, Durka Maxime, SOENS Hugo, WANG Jianyun
Принадлежит:

Microcapsules, for inclusion in concrete, adapted to reduce the area of a defect by at least 45% in said concrete once a quantity of said microcapsules has ruptured, said micro-capsules each comprising: a polymeric shell encapsulating a liquid core, wherein the polymeric shell comprises a substantially impermeable polymer layer and the liquid core comprises carbonatogenic bacterial spores, and optionally bacterial nutrients, dispersed in a liquid medium. Also disclosed is a concrete composition comprising a quantity of such microcapsules, and a method of reducing the area of a defect in concrete. 139.-. (canceled)40. Microparticles , for inclusion in a concrete , concrete-based , or concrete-like material , said microparticles each comprising:a core, in the form of a porous solid and/or a liquid;carbonatogenic bacterial spores dissolved and/or dispersed in the core,wherein said microparticles are adapted to reduce, or to assist in the reduction of, the area of a defect in said material by carbonatogenesis by the carbonatogenic bacteria once a quantity of said microparticles has fractured or is exposed at an interface of the defect.41. Microparticles as claimed in claim 40 , further comprising:a shell surrounding the core.42. Microparticles as claimed in wherein the core of each of the microparticles comprises the porous solid.43. Microparticles as claimed in claim 42 , wherein liquid is present in substantially all of the pores of the porous solid claim 42 , and the carbonatogenic bacterial spores are dispersed in the liquid and bacterial nutrients are dispersed or dissolved in the liquid.44. Microparticles as claimed in 41 claim 42 , wherein the shell further comprises a polymer layer claim 42 , the polymer further comprising melamine formaldehyde resin claim 42 , the polymer having reactive functional groups claim 42 , extending outwardly of the microparticle claim 42 , whereby the microparticle is chemically bondable within the material.45. Microparticles as ...

Подробнее
Номер записи: 65
14-01-2016 дата публикации

Methods of Patterning Cells on a Surface of a Substrate and Programmed Assembly of Three-Dimensional Living Tissues

Номер: US20160010054A1
Автор: Gartner Zev Jordan, Jee Noel Youngho, Liu Jennifer S., Todhunter Michael E.
Принадлежит:

The present disclosure provides methods of patterning cells on a surface of a substrate. The methods include disposing a pattern of nucleic acids on a surface of a substrate, and contacting the patterned nucleic acids under hybridization conditions with a first suspension of cells, where cells of the first suspension include cell surface-attached nucleic acids complementary to the patterned nucleic acids, and where the cell surface-attached nucleic acids hybridize to the patterned nucleic acids to pattern the cells on the surface of the substrate. Systems and kits for practicing the methods are also provided. 1. A method of patterning cells on a surface of a substrate , comprising:disposing a pattern of nucleic acids on a surface of a substrate; andcontacting the patterned nucleic acids under hybridization conditions with a first suspension of cells, wherein cells of the first suspension comprise cell surface-attached nucleic acids complementary to the patterned nucleic acids, and wherein the cell surface-attached nucleic acids hybridize to the patterned nucleic acids to pattern the cells on the surface of the substrate.2. The method according to claim 1 , wherein the pattern of nucleic acids comprises a single population of nucleic acids having the same nucleotide sequence.3. The method according to claim 1 , wherein the pattern of nucleic acids comprises two or more population of nucleic acids claim 1 , wherein each population of nucleic acids comprises a unique nucleotide sequence.4. The method according to claim 3 , wherein the nucleic acids of each population are uniquely addressable on the surface of the substrate.5. The method according to claim 3 , wherein the first suspension of cells comprises two or more populations of cells claim 3 , wherein each population of cells comprises surface-attached nucleic acids complementary to one of the populations of nucleic acids in the pattern.6. The method according to claim 5 , wherein each population of cells of the ...

Подробнее
Номер записи: 66
14-01-2016 дата публикации

Covalent Attachment of Bacteriophages (Phages) to Polymeric Surfaces

Номер: US20160010077A1
Автор: Elasri Mohamed o, Urban Marek W.
Принадлежит: THE UNIVERSITY OF SOUTHERN MISSISSIPPI

We disclose a method of covalently attaching bacteriophages to a surface, including polymers, to create a resulting antibacterial surface device. Because the bacteriophages are specific for bacteria, other organisms for which the phages are not specific are not damaged by the phage-modified surfaces. 1. A method for covalently attaching a bacteriophage to a surface comprising:a. providing a surface;b. reacting said surface with a carboxylic acid containing compound to provide carboxylic acid groups covalently attached to said surface;c. providing at least one bacteriophage; andd. covalently bonding said at least one bacteriophage to at least one of said carboxylic acid groups on said surface.2. The method of claim 1 , wherein said surface is a silicon wafer.3. The method of claim 1 , wherein said surface is a polymeric surface.4. The method of claim 3 , wherein said polymeric surface is made from an organic polymer.5. The method of claim 4 , wherein said organic polymer is selected from the group consisting of PE and PTFE.6. The method of claim 1 , wherein said carboxylic acid containing compound is maleic acid.7. The method of claim 6 , wherein the step of reacting said surface with a carboxylic acid containing compound to provide carboxylic acid groups covalently attached to said surface includes a microwave plasma reaction in the presence of maleic acid.8. The method of claim 7 , further comprising the step of incubating said surface with carboxylic acid groups covalently attached to said surface with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide.9. The method of claim 1 , wherein said at least one bacteriophage is at least one non-enveloped bacteriophage.10. The method of claim 9 , wherein said at least one non-enveloped bacteriophage is a T1 phage.11. The method of claim 1 , wherein said at least one bacteriophage is at least one enveloped bacteriophage.12. The method of claim 11 , wherein said at least one enveloped bacteriophage is a ...

Подробнее
Номер записи: 67
11-01-2018 дата публикации

LIGAND-BINDING FIBER AND CELL CULTURE SUBSTRATE USING SAID FIBER

Номер: US20180010115A1
Автор: AIHARA Ayako, Iwamoto Shunsuke, Kishioka Takahiro, Nishino Taito, Sakuma Daisuke, Umezaki Makiko
Принадлежит: NISSAN CHEMICAL INDUSTRIES, LTD.

The invention provides a ligand-bonded fiber in which a ligand having affinity for a cell membrane receptor is immobilized on a fiber precursor, and a cell culture substrate capable of repeating ex vivo amplification of a cell expressing a cell membrane receptor by using the ligand-bonded fiber. 1. A ligand-bonded fiber comprising a ligand having affinity for a cell membrane receptor , and a fiber precursor bonded to the ligand , thus forming a ligand-bonded fiber precursor (hereinafter to be referred to as a fiber precursor).2. The ligand-bonded fiber according to claim 1 , wherein the above-mentioned cell membrane receptor is a thrombopoietin (TPO) receptor.4. The ligand-bonded fiber according to claim 3 , wherein the above-mentioned ligand has an amino group claim 3 , and the amino group and the above-mentioned Qare bonded.6. The ligand-bonded fiber according to claim 3 , wherein the fiber precursor further comprises a crosslinking agent and an acid compound.7. The ligand-bonded fiber according to claim 3 , wherein the above-mentioned fiber precursor is produced by spinning a composition for producing a fiber precursor claim 3 , which composition comprising the above-mentioned polymer compound claim 3 , a crosslinking agent claim 3 , an acid compound and a solvent.8. The ligand-bonded fiber according to claim 7 , wherein the above-mentioned fiber precursor is produced by spinning the above-mentioned composition for producing a fiber precursor claim 7 , on a surface-treated substrate.9. The ligand-bonded fiber according to claim 3 , wherein the above-mentioned polymer compound has a weight average molecular weight of 1 claim 3 ,000-1 claim 3 ,000 claim 3 ,000.10. The ligand-bonded fiber according to claim 1 , wherein the above-mentioned fiber precursor is produced by heating at 70-300° C.12. The ligand-bonded fiber according to claim 11 , wherein Xis a 4-t-butylphenyl group claim 11 , and Xis an amino group.13. A cell culture substrate comprising the ligand-bonded ...

Подробнее
Номер записи: 68
14-01-2021 дата публикации

PROCESS FOR PREPARING A POLYMER/BIOLOGICAL ENTITIES ALLOY

Номер: US20210009765A1
Автор: Barbier Jacques, BATAILLE FREDERIC, Dever Cedric, FERREIRA THIERRY
Принадлежит:

The present invention relates to a process for preparing a polymer/biological entities alloy, comprising a step of mixing a polymer and biological entities that degrade it, during a heat treatment, said heat treatment being performed at a temperature T above room temperature and said biological entities being resistant to said temperature T, characterized in that said biological entities are chosen from enzymes that degrade said polymer and microorganisms that degrade said polymer. 1. A process for preparing a polymer/biological entities alloy , comprising:{'sub': 1', '6, 'a step of selecting a polymer from polytetramethylene succinate, copolyesters, polyesteramides, polypropylene, vinyl polymers, poly(C-Chydroxyalkanoates), poly(butylene adipate-co-terephthalate), poly(butylene succinate), polyamides, polyvinyl chloride, polyethylene, polypropylene, polyethylene terephthalate, or poly(trimethylene terephthalate) and mixtures thereof;'}a step of selecting enzymes that degrade said polymer; anda step of mixing said enzymes and said polymer during extrusion at a temperature (T) corresponding to the melting point of said polymer, the extrusion being performed in a twin screw extruder.2. The process as claimed in claim 1 , wherein the enzyme/polymer weight ratio is between about 0.1:100 and about 10:100.3. The process as claimed in claim 1 , wherein the enzyme/polymer weight ratio is between about 1:100 and about 3:100.4. The process as claimed in claim 1 , wherein the enzyme/polymer weight ratio is about 2:100.5. The process as claimed in claim 1 , wherein the twin-screw extruder is a co-rotating extruder.6. A polymer/enzyme alloy made by the process as claimed in . This application is a continuation of U.S. application Ser. No. 15/220,159, filed Jul. 26, 2016, which is a continuation application of U.S. application Ser. No. 14/308,526, filed Jun. 18, 2014, now U.S. Pat. No. 9,428,744, which is a continuation-in-part of International Patent Application No. PCT/FR2012/ ...

Подробнее
Номер записи: 69
14-01-2021 дата публикации

Systems and methods for growing a biofilm of probiotic bacteria on solid particles for colonization of bacteria in the gut

Номер: US20210009985A1
Автор: David Baram, David Daboush, Rachel Diamant
Принадлежит: Mybiotics Pharma Ltd

The present invention provides a method, wherein the method forms a biofilm, wherein the biofilm comprises a population of at least one bacterial strain attached to particles, wherein the biofilm is configured to colonize a gut of a subject in need thereof for at least five days, when ingested by the subject, the method comprising: a. obtaining a population comprising at least one strain of bacteria; b. inoculating a growth medium containing particles with the population comprising at least one strain of bacteria; c. incubating the particles with the population comprising at least one bacterial strain for a time sufficient for the population of at least one strain of bacteria to attach to the particles; and d. culturing the population comprising at least one strain of bacteria attached to the particles in a growth medium, for a time sufficient to form a biofilm.

Подробнее
Номер записи: 70
19-01-2017 дата публикации

Micro-Biologically Favoring Fungus Based Organic Biocomposite Substrate Having Superior Capillary Dynamics

Номер: US20170013792A1
Автор: Croteau Hans Steve
Принадлежит:

A soil-less biodegradable and porous rigid foam substrate for cultivating plants that has been formed of a heterogeneous matrix of organic fibers pasteurized with organic matter and natural minerals, and then contacted with a fungus, resulting in a biocomposite foam with excellent capillary dynamics, the whole of which does not compact and whose organic bio-composition favors the proper micro-biological activity upon a plants' rhizosphere. The preferred embodiment is to grow said substrate inside a waxed hexagonal prism shaped box, and around an externally accessible means of internal aeration, for approximately 3-5 days, and then to deactivate the live fungus in said biocomposite foam by exposure to microwave radiation. 1. A micro-biologically favoring fungus based biocomposite substrate having superior capillary dynamics comprising:a. one or more Fungi, and,b. one or more polymers, alone, or prepared with organic matter or natural minerals, or prepared with a combination of organic matter and natural minerals.2. The biocomposite substrate of claim 1 , further comprising a means of internal aeration.3. Said means of internal aeration of claim 2 , wherein said means of internal aeration is provided by a porous hose made of sintered rubber particles.4. Said means of internal aeration of claim 2 , wherein said means of internal aeration is provided by a porous hose made of materials that biodegrade under thermophilic activity.5. The biocomposite substrate of claim 1 , wherein said biocomposite substrate is mostly encapsulated by a container.6. The biocomposite substrate of claim 1 , wherein said one or more polymers is comprised of approximately 50% coco coir pith and approximately 50% rice hulls.7. The biocomposite substrate of claim 1 , wherein said organic matter includes approximately 0.5% in molasses of the total volume of said one or more polymers.8. The biocomposite substrate of claim 1 , wherein said natural minerals include approximately 0.12% in gypsum of ...

Подробнее
Номер записи: 71
03-02-2022 дата публикации

CONTINUOUS FLOW METHOD FOR PREPARING (R)-3-HYDROXY-5-HEXENOATE

Номер: US20220033863A1
Автор: CHEN Fener, CHENG Dang, Hu Chen, HUANG Huashan, HUANG Zedu, JIANG Meifen, LIU Minjie
Принадлежит:

Disclosed herein relates to biopharmaceuticals, and more particularly to a continuous flow method for preparing (R)-3-hydroxy-5-hexenoate. Carbonyl reductase and isopropanol dehydrogenase are co-immobilized onto an inert solid medium simultaneously to prepare a carbonyl reductase/isopropanol dehydrogenase co-immobilized catalyst, which is then filled into a microchannel reactor of the micro reaction system. A solution containing substrate 3-carbonyl-5-hexenoate is subsequently pumped into the microchannel reactor to perform an asymmetric carbonyl reduction reaction to obtain (R)-3-hydroxy-5-hexenoate. 2. The method of claim 1 , wherein in step (1) claim 1 , the inert solid medium is a composite material of polyvinyl alcohol and polyethylene glycol; and the step of “co-immobilizing a carbonyl reductase and an isopropanol dehydrogenase onto an inert solid medium simultaneously to prepare the co-immobilized catalyst” comprises:(a) preparing an aqueous solution of the polyvinyl alcohol and the polyethylene glycol; heating the aqueous solution until the aqueous solution becomes clear; and cooling the aqueous solution to 50° C. or less to obtain a first solution;(b) adding a crude carbonyl reductase solution and a crude isopropanol dehydrogenase solution into the first solution followed by uniform mixing to obtain a second solution; and(c) dropwise adding the second solution onto a polyethylene film; drying the polyethylene film at 35-40° C. for 0.5-1 hour to obtain the co-immobilized catalyst; and storing the co-immobilized catalyst at 4° C. for later use;wherein an amino acid sequence of the carbonyl reductase is shown in SEQ ID NO: 1; and an amino acid sequence of the isopropanol dehydrogenase is shown in SEQ ID NO: 2;a weight ratio of the polyvinyl alcohol to the polyethylene glycol is 5:1-3;the crude carbonyl reductase solution and the crude isopropanol dehydrogenase solution both have an initial concentration of 10%-30% (w/v); andin step (b), a volume ratio of the ...

Подробнее
Номер записи: 72
15-01-2015 дата публикации

NOVEL CROSSLINKING REAGENTS, MACROMOLECULES, THERAPEUTIC BIOCONJUGATES, AND SYNTHETIC METHODS THEREOF

Номер: US20150017246A1
Автор: Huang Yumei
Принадлежит: CellMosaic, Inc.

The invention provides novel chemical entities based on sugar alcohols. These new chemical entities are biocompatible and biodegradable. The molecules can be made in a single and pure form. The molecular weights of these molecules range from small (<1000 Da) to large (1000-120,000 Da). The sugar alcohol-based molecules can have functional groups throughout the molecule for crosslinking compounds, such as the preparation of antibody-drug conjugates, or to facilitate the delivery of therapeutic proteins, peptides, siRNA, and chemotherapeutic drugs. Also provided are new conjugate entities prepared through sugar alcohol molecules. Methods of synthesizing sugar alcohol-based molecules and conjugates are also within the scope of the invention. 120-. (canceled)22. The conjugate of claim 21 , wherein B comprises from 3 to about 1000 sugar alcohol units.23. The conjugate of claim 21 , wherein S is selected from the group consisting of polystyrene supports claim 21 , polyamide supports claim 21 , polyethylene glycol supports claim 21 , polyacrylic supports claim 21 , polyacrylic/beta-alanine copolymer supports claim 21 , polyacrylamide/polystyrene copolymer supports claim 21 , polyacrylamide/polyethylene glycol copolymer supports claim 21 , polyethyleneglyco/polystyrene copolymer supports claim 21 , controlled pore glass claim 21 , agarose claim 21 , dextran gel claim 21 , polysaccharide based polymer. a polymeric microsphere claim 21 , a latex microsphere claim 21 , a polymeric particle consist of polystyrene claim 21 , a polymeric particle consist of copolymers of styrene claim 21 , poly(methyl methacrylate) claim 21 , polyvinyltoluene claim 21 , poly(2-hydroxyethyl methacrylate) claim 21 , the copolymer of poly(2-hydroxyethyl methacrylate) claim 21 , poly(ethylene glycol dimethacrylate/2-hydroxyethylmetacrlate) claim 21 , poly(lactic-co-polycolic acid) claim 21 , inorganic constructs claim 21 , metals claim 21 , semiconductors claim 21 , super paramagnetic composites ...

Подробнее
Номер записи: 73
15-01-2015 дата публикации

Internally functionalized graphene substrates

Номер: US20150017699A1
Автор: Martinez William Emerson, McKinney Jeffrey Alan
Принадлежит: NANOTECH BIOMACHINES, INC.

The present invention is generally directed to internally functionalized graphene substrates, methods of making such substrates and methods of using such substrates. In one aspect, the present invention is a graphene substrate. The substrate comprises edge and non-edge regions. Organic or inorganic molecules are bound to the non-edge regions of the substrate, and the organic or inorganic molecules are present on the substrate edges at a population greater than about one molecule per 10,000 nm. 1. A graphene substrate , wherein the substrate comprises edge and non-edge regions , and wherein organic or inorganic molecules are bound to the non-edge regions of the substrate , and wherein the organic or inorganic molecules are present on the non-edge regions at a population greater than about one molecule per 30 ,000 nm.2. The graphene substrate according to claim 1 , wherein organic molecules are present on the substrate claim 1 , and wherein the organic molecules are selected from a group consisting of: antibodies; antibody fragments; aptamers; large molecule therapeutics; oligonucleotides; oligopeptides; oligosaccharides claim 1 , proteins and small molecule therapeutics.3. The graphene substrate according to claim 2 , wherein the population is greater than about 1 molecule per 10 claim 2 ,000 nm.4. The graphene substrate according to claim 3 , wherein the organic molecules are selected from a group consisting of: antibodies; antibody fragments; proteins; and claim 3 , aptamers.5. The graphene substrate according to claim 3 , wherein the organic molecules are selected from a group consisting of: large molecule therapeutics; oligonucleotides; oligopeptides; oligosaccharides and small molecule therapeutics.6. The graphene substrate according to claim 4 , wherein the population is greater than about 1 molecule per 3 claim 4 ,000 nm.7. The graphene substrate according to claim 5 , wherein the population is greater than about 1 molecule per 3 claim 5 ,000 nm.8. The ...

Подробнее
Номер записи: 74
16-01-2020 дата публикации

Cell mass or cell structure-embedding agent, cell mass or cell structure-containing composition, and kit

Номер: US20200015475A1
Автор: Kentaro Nakamura, Yasuhiro Yoshioka
Принадлежит: Fujifilm Corp

The object of the present invention is to provide a cell mass or cell structure-embedding agent that can stably transport cell masses or cell structures in a case of transportation at a low temperature and after transportation, conveniently recover a cell mass or cell structure from the cell mass or cell structure-embedding agent, a cell mass or cell structure-containing composition, and a kit. According to the present invention, provided is a cell mass or cell structure-embedding agent including: polypeptide which is represented by Formula 1 and in which an area of the maximum molecular weight peak in molecular weight distribution measurement is 80% or more of the total area of all of the molecular weight peaks. A-[(Gly-X-Y) n ] m -B  Formula (1): In the formula, X's and Y's each independently represent an amino acid, m is an integer of 2 to 10, n is an integer of 3 to 100, and A and B represent any amino acids or amino acid sequences.

Подробнее
Номер записи: 75
03-02-2022 дата публикации

DEVICES AND METHODS FOR SINGLE CELL ANALYSIS

Номер: US20220034867A1
Автор: Butler John, Chaudhuri Bidhan
Принадлежит:

The present disclosure provides systems, methods, and devices for the simultaneous determination of a single cell's response to a stimuli and characterization of its cell response. The present disclosure further provides methods for detection of disease state, clinical management of a subject suffering from a disease, drug screening, prediction of drug response, and stands to help direct drug and diagnostic development for the treatment of disease. 1. (canceled)2. A method of agent transfer comprising:providing a plurality of cells from a biological sample comprising a heterogenous population of cells from a patient positioned on a surface of a first solid substrate at a plurality of cell-philic sites;immobilizing a portion of the cells in a hydrogel concentration at their respective cell-philic sites thereby creating a plurality of three-dimensional cell microenvironments comprising the hydrogel composition and the cells, the hydrogel composition of each three-dimensional cell microenvironment being sized and positioned so as to extend upward with a dome shape from the surface of the solid substrate;dispending an agent onto an agent transfer device comprising a plurality of agent transfer array elements positioned on a surface of a second solid substrate; andcontacting the plurality of agent transfer array elements to the plurality of three-dimensional cell microenvironments, such that each agent transfer array element corresponds to a three-dimensional cell microenvironment, wherein the contacting allows the agent to be transferred to the plurality of three-dimensional cell microenvironments,3. The method of claim 2 , wherein the agent transfer device transfers at least 50% of the original concentration of the agent on the agent transfer device to the plurality of three-dimensional cell microenvironments.4. The method of claim 2 , wherein the plurality of cells comprise a clinical sample.5. The method of claim 2 , wherein the plurality of cells comprise a tumor ...

Подробнее
Номер записи: 76
18-01-2018 дата публикации

Nanocaged Enzymes with Enhanced Catalytic Activity and Increased Stability

Номер: US20180016569A1
Автор: Fu Jinglin, WOODBURY Neal, YAN Hao, ZHAO Zhao
Принадлежит:

The present disclosure describes a nanoparticle comprising a three dimensional DNA nanocage and a payload biological macromolecule, and methods of assembly thereof. 1. A nanocage comprising;a three dimensional body comprising a plurality of structural members comprising M13 viral DNA, wherein internal surfaces of the plurality of structural members form an inner cavity, wherein architectural arrangement of the structural members in the three dimensional body forms an arrangement selected from the group consisting of a honeycomb lattice, a single-walled square lattice, and a double-walled square lattice.2. The nanocage of claim 1 , wherein the three dimensional body is smaller than 100 nm×100 nm×100 nm.3. The nanocage of claim 2 , wherein the three dimensional body is smaller than 75 nm×50 nm×50 nm.4. The nanocage of claim 1 , wherein the inner cavity of the three dimensional body measures less than 50 nm×50 nm×50 nm.5. The nanocage of claim 1 , wherein the three dimensional body further comprises at least one nanopore.6. The nanocage of claim 5 , wherein the at least one nanopore has a diameter of about 1 nm to about 5 nm.7. The nanocage of claim 5 , wherein the at least one nanopore has a diameter of about 1.5 nm to about 3 nm.8. The nanocage of claim 1 , wherein the three dimensional body comprises between 0.10 to 0.30 DNA helices per nm.9. The nanocage of claim 1 , wherein the three dimensional body comprises between 0.11 to 0.17 DNA helices per nm.10. A nanoparticle comprising:a nanocage comprising a plurality of structural members comprising DNA in a three-dimensional lattice, wherein internal surfaces of the plurality of structural members form an inner cavity; andone or more payload molecules bound to internal surfaces of the inner cavity, wherein the one or more payload molecules comprise enzymes, nucleic acids, polypeptides, antibodies, phospholipids, or any combination thereof.11. The nanoparticle of claim 10 , wherein the inner cavity encapsulates two ...

Подробнее
Номер записи: 77
21-01-2021 дата публикации

AUTOMATED BIONANOCATALYST PRODUCTION

Номер: US20210016263A1
Автор: Brooks Rani Talal, Chairil Ricki, CHUN Matthew, Corgie Stephane
Принадлежит: ZYMtronix, LLC

The present invention provides machines, compositions and methods for producing bionanocatalysts (BNCs) comprising one or more enzymes selected from a broad spectrum of industrially and medically important enzymes. The BNCs are self-assembled and magnetically immobilized enzymes. The machines, compositions, and methods are fully scalable from bench top to industrial manufacturing volumes. 2. The machine of claim 1 , wherein said MNP disruptor is a sonicator.3. The machine of claim 2 , wherein said sonicator further comprises a sonicator coil and a sonication container claim 2 , wherein said sonicator coil is operable to sonicate said MNPs within said sonication container.4. The machine of claim 2 , wherein the sonicator is an in-line sonicator.5. The machine of claim 2 , further comprising a cooling system operable for cooling said sonicator.6. The machine of claim 5 , wherein said cooling system is a water cooling system.7. The machine of claim 1 , wherein said MNP disruptor is operable to mechanically disrupt said MNPs.8. The machine of claim 1 , wherein said MNP disruptor is operable to magnetically disrupt said MNPs.9. The machine of claim 1 , wherein said MNP disruptor is operable to thermally disrupt said MNPs.10. The machine of claim 1 , wherein said BNC mixer comprises a mixing tee.11. The machine of claim 1 , wherein said BNC mixer comprises a mixing coil.12. The machine of claim 1 , wherein said enzyme pump sends said enzyme preparation to said BNC mixer via mechanical or gravitational force.13. The machine of claim 1 , wherein said MNP pump sends said MNP preparation to said MNP disruptor via mechanical or gravitational force.14. The machine of claim 1 , further comprising a magnetic scaffolding container operable for mixing a magnetic scaffolding preparation with said BNCs in said scaffolding container to produce BNCs in a level 2 assembly.15. The machine of claim 14 , wherein said magnetic scaffolding container is operable to mix said BNCS and said ...

Подробнее
Номер записи: 78
17-04-2014 дата публикации

Microorganisms-immobilized felt-based resin for the treatment of phenolic effluents and a preparation method thereof

Номер: US20140102979A1
Автор: Jianmei Lu, Najun Li
Принадлежит: Suzhou Timelyblue Environmental Tech Co Ltd

A method of preparing a microorganisms-immobilized felt-based resin includes the following steps: providing a mixture of an acrylate monomer, an initiator, a solvent, and water; adding a felt to the mixture; initiating a polymerization reaction of the mixture to form a felt-based resin; and immobilizing microorganisms on the felt-based resin to form the microorganisms-immobilized felt-based resin.

Подробнее
Номер записи: 79
21-01-2021 дата публикации

METHOD FOR PREPARING SALIDROSIDE

Номер: US20210017560A1
Автор: CUI LANGJUN, GENG YINGJUN, GUI JICHENG, LIU MAODONG, MAO ZHAOLIN, ZHAO CAIHONG
Принадлежит:

The present invention provides a method for preparing salidroside. The present invention uses β-glucoside and CoFeOparticles to form a cross-linked aggregate capable of effectively catalyzing the reaction of β-D-ghucose and tyrosol, thereby increasing the yield of the salidroside. The steps of the preparation method of the present invention are simple and short, and the method is easy to operate and readily applicable to industrial production. 1. A method for preparing salidroside , comprising the following steps:{'sub': 2', '4, '(1) adding β-glucosidase into a phosphate-citric acid buffer solution, adding polyacrylamide cross-linked hollow CoFeOparticles, adding a settling agent, glutaraldehyde and sodium borohydride after oscillating, oscillating, centrifuging same at 320-480 rpm for 5-10 min, feeding same into a thermostatic water bath at 40-45° C., keeping the temperature and stirring same for 1-2 h, discharging, and collecting precipitates to obtain a β-glucosidase cross-linked aggregate;'}(2) adding β-D-glucose and tyrosol into a solvent, adding the buffer solution and the β-glucosidase cross-linked aggregate obtained in the step (1), and reacting to obtain a reaction solution; and(3) filtering the reaction solution obtained in the step (2), and carrying out reduced pressure distillation on a filtrate to obtain a crude product; and recrystallizing the crude product to obtain the salidroside.2. The method for preparing the salidroside according to claim 1 , wherein a method for preparing the polyacrylamide cross-linked hollow CoFeOparticles comprises the following steps:(1) adding 1-2 parts by weight of ammonium persulfate into deionized water which is 20-30 times of the weight of the ammonium persulfate, and uniformly stirring same; and{'sub': 2', '4, '(2) adding 40-45 parts by weight of methyl methacrylate into the deionized water which is 5-8 times of the weight of the methyl methacrylate, uniformly stirring same, feeding same into a reaction kettle, ...

Подробнее
Номер записи: 80
28-01-2016 дата публикации

CELL-BASED COMPOSITIONS, CELL-BASED BANDAGE DEVICES AND SYSTEMS AND METHODS OF TREATMENT THEREWITH

Номер: US20160022872A1
Автор: TAYLOR DONALD P., Wells Alan, YATES-BINDER CECELIA CHRISTINA
Принадлежит:

A composition includes a biodegradable matrix, cells within the biodegradable matrix and at least one bioactive agent having limited mobility relative to the cells within the biodegradable matrix. The at least one bioactive agent is bioactive within the biodegradable matrix for regulation of the cells to enhance cell survival. 1. A composition comprising a biodegradable matrix , cells within the biodegradable matrix and at least one bioactive agent having limited mobility relative to the cells within the biodegradable matrix , wherein the at least one bioactive agent is bioactive within the biodegradable matrix for regulation of the cells to enhance cell survival.2. The composition of wherein the at least one bioactive agent is a matrikine or a matrikine fragment which is immobilized upon the cells or is immobilized within the biodegradable matrix.3. The composition of wherein the at least one bioactive agent is a matrikine or a matrikine fragment.4. The composition of wherein the cells are cells which can differentiate into at least one other cell type.5. The composition of wherein the cells are multipotent.6. The composition of wherein the cells include at least one of mesenchymal stem cells claim 4 , tissue stromal cells claim 4 , tissue epithelial cells or endothelial progenitor cells.7. The composition of further comprising cells or another agent that facilitates or directs differentiation.8. The composition of wherein the matrikine or the matrikine fragment is tethered to the biodegradable matrix.9. The composition of wherein the matrikine or the matrikine fragment is covalently bonded to the biodegradable matrix.10. The composition of wherein the matrikine or the matrikine fragment is non-covalently bonded to the biodegradable matrix.114. The composition of wherein the matrikine or the matrikine fragment is adhered to the cells.12. The composition of wherein the matrikine or the matrikine fragment is selected from the group of a tenascin claim 4 , a laminin ...

Подробнее
Номер записи: 81
22-01-2015 дата публикации

Oil Absorption and Bioremediation Apparatus Employing Oil Digesting MIcroorganisms

Номер: US20150024454A1
Автор: Hondroulis Dimitrios, Johnson, JR. Troy W.
Принадлежит:

An absorbent material may include banana tree stalk fibers separated and processed for moisture content reduction to generate processed fibers, and oil eating microorganisms (OEMs) infused into the processed fibers to form OEM infused fibers. The processed fibers may be configured to concentrate oil therein responsive to exposure to the oil. The OEMs may remain in a dormant state in the OEM infused fibers until activated by water and exposed to the oil. 1. A process for producing absorbent materials , the process comprising:reducing a banana tree stalk into separated fibers;processing the separated fibers to reduce moisture content thereof to generate processed fibers;infusing oil eating microorganisms (OEMs) into the processed fibers to create OEM infused fibers; andincorporating the OEM infused fibers into an absorbent material.2. The process of claim 1 , wherein processing the separated fibers further includes pressing the separated fibers using a hydraulic press claim 1 , a screw press or a belt press to remove water claim 1 , latex claim 1 , or natural juices.3. The process of claim 1 , wherein processing the separated fibers includes one or more of washing claim 1 , treating claim 1 , pre-drying and drying the separated fibers.4. The process of claim 3 , wherein drying the separated fibers employs one or more of heat claim 3 , airflow and kinetic energy application methods.5. The process of claim 1 , wherein infusing the OEMs comprises infusing OEMs that are in a dormant state in the OEM infused fibers until activation by water and exposure to a petroleum-related product occurs.6. The process of claim 5 , wherein incorporating the OEM infused fibers into the absorbent material comprises incorporating the OEM infused fibers into one or more of a loose absorbent material claim 5 , a wipe claim 5 , a rag claim 5 , a towel claim 5 , a pillow or a boom.7. The process of claim 5 , wherein the processed fibers concentrate the petroleum product and the OEMs digest the ...

Подробнее
Номер записи: 82
24-01-2019 дата публикации

Cross-Linked Poly-E-Lysine Non-Particulate Support

Номер: US20190023844A1
Автор: Donald A. Wellings
Принадлежит: SPHERITECH LTD

The invention provides a non-particulate cross-linked poly-ε-lysine polymer. The poly-ε-lysine and cross linker are linked by amide bonds and may the cross linker has at least two functional groups capable of reacting with an alpha carbon amine of poly-ε-lysine. The polymer is suitably insoluble in water and other solvents and is provided in macro form for example a sheet, article or fibre. The macro form polymer is useful in a wide range of applications including wound treatment, as a medical diagnostic comprising a particulate support and a functional material bound or retained by the support and solid phase synthesis of peptides, oligonucleotides, oligosaccharides, immobilisation of species, cell culturing and in chromatographic separation.

Подробнее
Номер записи: 83
24-01-2019 дата публикации

NAD(P)- Dependent Responsive Enzymes, Electrodes And Sensors, And Methods For Making And Using The Same

Номер: US20190024130A1
Автор: Feldman Benjamin J., Ouyang Tianmei
Принадлежит: ABBOTT DIABETES CARE INC.

Embodiments of the present disclosure relate to NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Also provided are methods for making the compositions and for detecting and/or measuring analytes with NADP-dependent oxidoreductase compositions. 1. An enzyme composition comprising:{'sup': '+', 'nicotinamide adenine dinucleotide phosphate (NAD(P)) or derivative thereof;'}{'sup': '+', 'an NAD(P)-dependent dehydrogenase;'}an NAD(P)H oxidoreductase; andan electron transfer agent comprising a transition metal complex.2. The enzyme composition according to claim 1 , wherein the composition comprises nicotinamide adenine dinucleotide phosphate (NAD(P)).3. The enzyme composition according to claim 1 , wherein the composition comprises a derivative of nicotinamide adenine dinucleotide phosphate.6. The enzyme composition according to claim 1 , wherein the NAD(P)-dependent dehydrogenase is selected from the group consisting of glucose dehydrogenase claim 1 , alcohol dehydrogenase claim 1 , D-3-hydroxybutyrate dehydrogenase.7. The enzyme composition according to claim 1 , wherein the NAD(P)H oxidoreductase is diaphorase.8. The enzyme composition according to claim 1 , wherein electron transfer agent comprises an osmium-containing complex.9. The enzyme composition according to claim 8 , wherein the osmium-containing complex comprises three heterocyclic nitrogen-containing bidentate ligands.10. The enzyme composition according to claim 9 , wherein the osmium-containing complex comprises two biimidazole and one imidazole-pyridine bidentate ligands.11. The enzyme composition according to claim 1 , wherein the composition comprises a heterocycle-containing polymer and a crosslinker.12. The enzyme composition according to claim 11 , wherein the heterocycle-containing polymer comprises polyvinylpyridine.13. The enzyme composition according to claim 11 , wherein one or more of the nicotinamide adenine dinucleotide phosphate (NAD(P)) ...

Подробнее
Номер записи: 84
23-01-2020 дата публикации

PHOTOBIOREACTOR SYSTEMS AND METHODS

Номер: US20200024559A1
Автор: Gross Martin Anthony, Wen Zhiyou
Принадлежит:

An algal growth system includes a first flexible sheet material mounted on a first frame in a first mounted geometry having a first height and a first width, the first height being greater than the first width, and a second flexible sheet material mounted on a second frame in a second mounted geometry having a second height and a second width, the second height being greater than the second width. The first flexible sheet and the second flexible sheet material are noncontiguous. The algal growth system also includes a motor, the motor being coupled with an actuator system, where the motor actuates the actuator system, and a reservoir. 1. A biomass growth system comprising:a. a frame;b. a first flexible sheet material mounted on a first frame in a first mounted geometry having a first height and a first width, wherein the first height is greater than the first width;c. a second flexible sheet material mounted on a second frame in a second mounted geometry having a second height and a second width, the first flexible sheet material and the second flexible sheet material being noncontiguous, wherein the second height is greater than the second width;d. a first drive shaft, the first drive shaft being coupled with the first frame, wherein the first drive shaft actuates the first flexible sheet material, and a second drive shaft, the second drive shaft being coupled with the second frame, wherein the second drive shaft actuates the second flexible sheet material;e. an actuator system, wherein the actuator system is coupled with the first drive shaft and the second drive shaft such that the first flexible sheet material and the second flexible sheet material are concurrently actuated;f. a motor, the motor being coupled with the actuator system, wherein the motor actuates the actuator system and the first drive shaft such that the first flexible sheet material is actuated and the second drive shaft such that the second flexible sheet material is actuated concurrently;g. a ...

Подробнее
Номер записи: 85
28-01-2021 дата публикации

Microorganism separation and detection

Номер: US20210024877A1
Автор: Andrew Rogers, Daniel Lockhart, James Turner, Matthew Crow, Paul Jay, William Mullen
Принадлежит: Momentum Bioscience LTD

Methods for separating microorganisms from non-microorganism cells in a non-microorganism cell-containing sample comprise incubating the sample with particles to form particle-microorganism complexes and then separating the particle-microorganism complexes from the non-microorganism cells. These methods are used to detect the absence or presence of a microorganism in a sample that also contains non-microorganism cells. Particular reagents and combinations of reagents enhance the selective capture of microorganisms in mixed samples. Corresponding compositions and kits are also provided.

Подробнее
Номер записи: 86
28-01-2021 дата публикации

DEVICE

Номер: US20210025870A1
Автор: Aiba Kazuhiro, Hori Kosuke, Sudo Masaki, Tooi Norie
Принадлежит:

The present invention provides a nerve cell device in which early observation of nerve activity (spikes, bursts, and the like) is made possible and the measured electric strength is increased by cultivating neurons upon a cell scaffold. By using this nerve cell device, imaging of intracellular signaling is also possible. 1. A nerve cell device comprising a cell scaffold and neurons.2. The nerve cell device according to claim 1 , wherein the neurons are oriented.3. The nerve cell device according to claim 1 , wherein the cell scaffold is a fiber sheet formed of a polymeric material.4. The nerve cell device according to claim 3 , wherein the fiber sheet has an oriented structure claim 3 , a non-oriented structure or a mixed structure of orientation and non-orientation.5. The nerve cell device according to claim 3 , wherein the fiber sheet is coated with the extracellular matrix protein selected from polylysine claim 3 , polyornithine claim 3 , laminin claim 3 , fibronectin claim 3 , MATRIGEL® and GELTREX®.6. The nerve cell device according to claim 1 , wherein the neurons form a three-dimensional structure on the cell scaffold and/or in the cell scaffold.7. The nerve cell device according to claim 1 , wherein the neurons are neural cells derived from primary cultured cells or pluripotent stem cells.8. The nerve cell device according to claim 7 , wherein the neural cells derived from the primary cultured cells or pluripotent stem cells are neural cells derived from mammals.9. The nerve cell device according to claim 1 , wherein the neurons comprise glutamatergic claim 1 , dopaminergic claim 1 , gamma-aminobutyratergic claim 1 , monoaminergic claim 1 , histaminergic or cholinergic neurons.10. The nerve cell device according to claim 1 , wherein: the neurons are seeded at a density of 1×10cells/cmto 4×10cells/cmagainst the cell scaffold.11. The nerve cell device according to claim 1 , further comprising: a frame for holding the periphery of the nerve cell device.12. The ...

Подробнее
Номер записи: 87
04-02-2016 дата публикации

CONTAINER WITH BIOFILM FORMATION-INHIBITING MICROORGANISMS IMMOBILIZED THEREIN AND MEMBRANE WATER TREATMENT APPARATUS USING THE SAME

Номер: US20160030890A1
Автор: Choi Dong-Chan, Lee Chung-Hak, Lee Kibaek, Lee Sang Hyun, Lee Seon-Ki
Принадлежит:

The present disclosure relates to a technique for inhibiting biofouling of the surface of a membrane caused by a biofilm, through immobilizing biofilm formation-inhibiting microorganisms to a container in a membrane water treatment process. The present disclosure provides a non-hollow/hollow columnar or sheet-like permeable carrier with flowability owing to submerged aeration and a container with biofilm formation-inhibiting microorganisms immobilized therein, comprising biofilm formation-inhibiting microorganisms immobilized in the carrier. The present disclosure also provides a membrane water treatment apparatus comprising a reactor accommodating water to be treated, a membrane module for water treatment and a container with biofilm formation-inhibiting microorganisms immobilized therein placed in the reactor. 1. A container with biofilm formation-inhibiting microorganisms immobilized therein , comprising a columnar or sheet-like permeable carrier having fluidisability through submerged aeration and biofilm formation-inhibiting microorganisms immobilized in the carrier.2. The container with biofilm formation-inhibiting microorganisms immobilized therein according to claim 1 , wherein the permeable carrier is a circular columnar carrier.3. The container with biofilm formation-inhibiting microorganisms immobilized therein according to claim 1 , wherein the permeable carrier is a hollow columnar carrier.4. The container with biofilm formation-inhibiting microorganisms immobilized therein according to claim 1 , wherein the columnar permeable carrier has an aspect ratio of around 5-500 claim 1 , the aspect ratio being a ratio of a maximum diameter of a cross-section to length thereof.5. The container with biofilm formation-inhibiting microorganisms immobilized therein according to claim 1 , wherein the sheet-like permeable carrier has a ratio of surface area to volume (SAN) of around 5-1 claim 1 ,000 mm.6. The container with biofilm formation-inhibiting microorganisms ...

Подробнее
Номер записи: 88
01-02-2018 дата публикации

CONTAINER WITH BIOFILM FORMATION-INHIBITING MICROORGANISMS IMMOBILIZED THEREIN AND MEMBRANE WATER TREATMENT APPARATUS USING THE SAME

Номер: US20180028982A1
Автор: Choi Dong-Chan, Lee Chung-Hak, Lee Kibaek, Lee Sang Hyun, Lee Seon-Ki
Принадлежит: SNU R&DB FOUNDATION

The present disclosure relates to a technique for inhibiting biofouling of the surface of a membrane caused by a biofilm, through immobilizing biofilm formation-inhibiting microorganisms to a container in a membrane water treatment process. The present disclosure provides a non-hollow/hollow columnar or sheet-like permeable carrier with flowability owing to submerged aeration and a container with biofilm formation-inhibiting microorganisms immobilized therein, comprising biofilm formation-inhibiting microorganisms immobilized in the carrier. The present disclosure also provides a membrane water treatment apparatus comprising a reactor accommodating water to be treated, a membrane module for water treatment and a container with biofilm formation-inhibiting microorganisms immobilized therein placed in the reactor. 1. A membrane bio-reactor (MBR) process for water treatment , comprising:contacting water to be treated with microorganisms for biological water treatment; andfiltering the biologically treated water through the membrane in the presence of a container comprising a permeable carrier and biofilm formation-inhibiting microorganisms, other than the microorganisms for biological water treatment, immobilized in the carrier,wherein the biofilm formation-inhibiting microorganisms inhibit quorum sensing of the microorganisms for biological water treatment responsible for biofilm formation on a surface of the membrane; andwherein the permeable carrier comprises a hydrogel and has fluidisability through submerged aeration.2. A membrane bio-reactor (MBR) process for water treatment according to claim 1 , wherein the permeable carrier is a spherical in shape.3. A membrane bio-reactor (MBR) process for water treatment according to claim 1 , wherein the permeable carrier is a columnar or sheet-like in shape.4. A membrane bio-reactor (MBR) process for water treatment according to claim 3 , wherein the permeable carrier is a circular columnar in shape.5. A membrane bio- ...

Подробнее
Номер записи: 89
02-02-2017 дата публикации

Methods, compositions and devices for maintaining chemical balance of chlorinated water

Номер: US20170029304A1
Автор: Vivian I. Teichberg
Принадлежит: Mia Levite, Nof Lyle Teichberg, Yaar Teichberg

A composition-of-matter for use in water treatment, composed of a water-insoluble matrix and one or more amidohydrolase, such as cyanuric acid amidohydrolase, incorporated in or on the matrix, is disclosed. Also disclosed are devices containing same and methods utilizing same for water treatment. The water treatment is effected by an enzymatically-catalyzed reduction of the concentration of an amide-containing compound, such as cyanuric acid, found in chlorinated water of swimming polls, spas and other similar structures.

Подробнее
Номер записи: 90
04-02-2016 дата публикации

APPARATUS AND PROCESS FOR PRODUCTION OF AN ENCAPSULATED CELL PRODUCT

Номер: US20160032270A1
Автор: Beckler Andersen Lisa, Evans John H., Singer Christine A.
Принадлежит: GEOSYNFUELS, LLC

A process for production of an encapsulated cell product, the process comprises the steps of concentrating cells from a propagation medium using a tangential flow filtration system. Mixing the concentrated cells with an encapsulation medium to form a cell encapsulation mixture. Polymerizing, gelling, or cross-linking the cell encapsulation mixture to form an encapsulated cell product. 1. A process for production of an encapsulated cell product , the process comprising the steps of:a. concentrating cells from a propagation medium using a tangential flow filtration system;b. mixing concentrated cells with an encapsulation medium to form a cell encapsulation mixture; andc. polymerizing the cell encapsulation mixture to form an encapsulated cell product.2. The process of claim 1 , wherein the cells are concentrated in the retentate of the tangential flow filtration system.3. The process of claim 1 , further comprising the step of using retentate from the tangential flow filtration system as an inoculum for a subsequent production of an encapsulated cell product.4. The process of claim 2 , further including the step of cycling the retentate between a retentate holding vessel and the tangential flow filtration system.5. The process of claim 4 , wherein the cycling step is performed until the retentate achieves a cell concentration of greater than 180 grams wet mass of cells per liter.6. The process of claim 1 , wherein the mixing step is performed with a reciprocating shaker.7. The process of claim 1 , wherein the mixing step is performed with a reciprocating disk.810-. (canceled)11. A process for production of an encapsulated cell product claim 1 , the process comprising the steps of:a. concentrating cells from a propagation medium;b. mixing the concentrated cells with an encapsulation medium using a reciprocating shaker to form a cell encapsulation mixture; andc. polymerizing the cell encapsulation mixture to form an encapsulated cell product.12. The process of claim 11 ...

Подробнее
Номер записи: 91
04-02-2016 дата публикации

ENCAPSULATION OF BACTERIA AND VIRUSES IN ELECTROSPUN FIBERS

Номер: US20160032271A1
Автор: KUHN Jonathan Charles, ZUSSMAN Eyal
Принадлежит: TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED

A method of preserving organisms in viable form, the method comprising: suspending organisms in a solution of electrospinnable polymer; drawing droplets of said solution through a spinneret; applying an electrostatic field to said droplets under electrospinning conditions; so as to form fibers having a diameter no greater than about 5 μm within which distinct organisms are encapsulated in viable form. 2. The method of claim 1 , wherein said biological matter is selected from the group consisting of bacteria and non-filamentous viruses.3. The method of claim 2 , wherein said solution further comprises an osmolarity-regulating agent.4. The method of claim 3 , wherein said osmolarity-regulating agent is selected from the group consisting of glycerol claim 3 , a polysaccharide polymer claim 3 , glycol claim 3 , and polyethylene glycol.5Escherichia coliStaphylococcus albus.. The method of claim 2 , wherein said bacteria comprises or6. The method of claim 2 , wherein said viruses are selected from the group consisting of bacterial viruses T7 claim 2 , T4 claim 2 , and λ claim 2 , Herpes simplex claim 2 , Cytomegalovirus claim 2 , Papilloma virus claim 2 , Adenovirus claim 2 , Burkitt lymphoma virus claim 2 , Arbovirus claim 2 , Arenavirus claim 2 , Epstein-Barr virus claim 2 , Varicella virus claim 2 , Cornavirus claim 2 , Coxsackievirus claim 2 , Eboli virus claim 2 , Enterovirus claim 2 , Hepatitis virus claim 2 , Influenza virus claim 2 , Marburg virus claim 2 , Measles virus claim 2 , Mumps virus claim 2 , Polio virus claim 2 , Rhinovirus claim 2 , Rubella virus claim 2 , Smallpox virus claim 2 , Rabies virus claim 2 , and Rotavirus.7E. coli. The method of claim 6 , wherein said bacterial viruses are grown on strain.8E. coli. The method of claim 7 , wherein said strain comprises K12.9. The method of claim 1 , wherein said spinneret is selected from the group consisting of a pipette and a syringe.10. The method of claim 9 , wherein said syringe further comprises a ...

Подробнее
Номер записи: 92
04-02-2016 дата публикации

PREPARATION METHOD OF YEAST CELL IMMOBILIZATION MEDIUM AND APPLICATION THEREOF

Номер: US20160032272A1
Автор: Chen Xiaochun, Chen Yong, LIU Qingguo, Wu Jinglan, Xie Jingjing, Ying Hanjie
Принадлежит:

Provided is a preparation method of a yeast cell immobilization medium, which comprises the following steps: (1) boiling a fiber material in boiling water and drying the fiber material; (2) soaking the fiber material in a surface modified aqueous solution with a concentration of 1-100 g/L, using hydrochloric acid to adjust a PH of the solution to 7.0, fully rinsing the fiber material in deionized water and drying the fiber material; (3) soaking the fiber material in a cross-linking agent aqueous solution with a concentration of 1-100 g/L, fully rinsing the fiber material in deionized water and drying the fiber material; and (4) attaching the fiber material to supporting framework. Also provided is the yeast cell immobilization medium prepared using the preparation method and a method for producing ethanol using the yeast cell immobilization medium. 1. A preparation method of a yeast cell immobilization medium , which comprises the following steps:(1) Boiling a fiber material in boiling water followed by drying;(2) Soaking the fiber material in a surface modifier aqueous solution at a concentration of 1-100 g/L, adjusting the pH of the solution to 7.0 with hydrochloric acid, and then fully rinsing the fiber material in deionized water followed by drying;(3) Soaking the fiber material in a cross-linking agent aqueous solution at a concentration of 1-100 g/L, and then fully rinsing the fiber material in deionized water followed by drying;(4) Attaching the fiber material to a supporting framework.2. The preparation method according to claim 1 , wherein said surface modifier is one or more selected from a group consisting of polyetherimide claim 1 , diethylenetriamine claim 1 , dimethylaminopropylamine claim 1 , polyethyleneimine and succinimide.3. The preparation method according to claim 1 , wherein said fiber material is one or more selected from a group consisting of cotton fabric; activated carbon fabric; polyester fiber; silk; bamboo fiber; polyvinyl alcohol fiber ...

Подробнее
Номер записи: 93
05-02-2015 дата публикации

SURFACE-TREATED CALCIUM CARBONATE FOR BINDING AND BIOREMEDIATING HYDROCARBON-CONTAINING COMPOSITIONS

Номер: US20150034554A1
Автор: Di Maiuta Nicola, Schwarzentruber Patrick, Skovby Michael
Принадлежит:

The invention relates to a surface-treated calcium carbonate for binding and bioremediating hydrocarbon-containing compositions, to a method for binding and bioremediating hydrocarbon-containing compositions as well as to the use of surface-treated calcium carbonate for binding and bioremediating hydrocarbon-containing compositions and to a composite material comprising the surface-treated calcium carbonate and a hydrocarbon-containing composition. 1. A surface-treated calcium carbonate for binding and bioremediating hydrocarbon-containing compositions having a degradation rate for the hydrocarbon-containing composition of at least 25% , based on the total weight of the hydrocarbon-containing composition , wherein at least 10% of the aliphatic carboxylic acid accessible surface area of the calcium carbonate is covered by a coating comprising at least one aliphatic carboxylic acid having between 5 and 24 carbon atoms and/or reaction products thereof.2. The surface-treated calcium carbonate according to claim 1 , wherein the surface-treated calcium carbonate comprises ground calcium carbonate and/or precipitated calcium carbonate and/or surface-modified calcium carbonate claim 1 , preferably ground calcium carbonate.3. The surface-treated calcium carbonate according to claim 2 , wherein the source of ground calcium carbonate (GCC) is selected from marble claim 2 , chalk claim 2 , calcite claim 2 , dolomite claim 2 , limestone and mixtures thereof and/or the precipitated calcium carbonate (PCC) is selected from one or more of the aragonitic claim 2 , vateritic and calcitic mineralogical crystal forms.4. The surface-treated calcium carbonate according to claim 1 , wherein the surface-treated calcium carbonate has a weight median particle diameter dvalue of between 0.1 μm and 250 μm claim 1 , preferably between 1 μm and 200 μm claim 1 , more preferably between 1 μm and 150 μm claim 1 , even more preferably between 1 μm and 100 μm and most preferably between 3 μm and 100 ...

Подробнее
Номер записи: 94
04-02-2021 дата публикации

ELECTROCHEMICALLY ENGINEERED SURFACE OF HYDROGELS, PARTICULARLY PEG HYDROGELS, FOR ENHANCED CELLULAR PENETRATION

Номер: US20210032412A1
Автор: EHRBAR Martin, MILLERET Vincent, SIMONA Benjamin R., Voros Janos
Принадлежит:

The invention relates to a polymer structure () formed by at least a polymer, wherein said structure () comprises a volume () and a surface (), wherein said polymer comprises a plurality of polymer chains connected by linkings, characterized by a linking density, wherein said linking density increases, particularly monotonously, from the surface () into the volume () of the polymer structure (). 1. A method for embedding cells into a polymer structure , providing a polymer structure formed by at least one polymer, wherein said polymer structure comprises a volume and a surface, wherein said polymer comprises a plurality of polymer chains connected by linkings, wherein the polymer structure is characterized by a linking density, wherein said linking density increases monotonously from the surface into the volume of the polymer structure, wherein the linking density is minimal at the surface and reaches a maximum in the volume, thereby forming a linking density gradient, and', 'seeding cells or cell aggregates comprising said cells on said surface of said polymer structure, such that the cells migrate into the volume of the polymer structure along said linking density gradient to become embedded within the volume., 'the method comprising the steps of2. The method according to claim 1 , wherein the linking density is zero at the surface.3. The method according to claim 1 , wherein the linking density reaches said maximum at a distance from the surface ranging between 1 μm and 1000 μm.4. The method according to claim 1 , wherein said linking density of the polymer structure increases monotonously along a gradient direction which is perpendicular to the surface.5. The method according to claim 4 , wherein said linking density of the polymer structure is uniform along the entire surface of the polymer structure.6. The method according to claim 1 , wherein said surface of the polymer structure extends along a horizontal direction.7. The method according to claim 1 , ...

Подробнее
Номер записи: 95
05-02-2015 дата публикации

COMPOSITIONS FOR CAUSING, ENHANCING, AND/OR EXPEDITING OXO-BIODEGRADATION OF ARTICLES AND METHODS OF PRODUCTION AND USE THEREOF

Номер: US20150037865A1
Автор: Weder Donald E.
Принадлежит:

Kits and assemblies for causing, enhancing, and/or expediting consumption of an article by at least one biodegradative living organism are disclosed. The kits and assemblies include at least one additional living organism that enhances and/or expedites consumption of the article by the at least one biodegradative living organism. Methods of producing and using same are also provided. 1. An assembly , comprising:an article formed of a material; anda composition disposed on at least a portion of the material, the composition comprising at least one agent of oxo-biodegradation, and wherein the composition causes, enhances, and/or expedites oxo-biodegradation of at least a portion of the treated material.2. The assembly of claim 1 , wherein the material from which at least a portion of the article is formed is a substantially non-biodegradable material.3. The assembly of claim 2 , wherein the composition is only disposed on a portion of the material claim 2 , and the remaining portion of the material is not substantially degraded by oxo-biodegradation.4. The assembly of claim 2 , wherein the composition is only disposed on a portion of the material claim 2 , and wherein the composition causes claim 2 , enhances claim 2 , and/or expedites oxo-biodegradation of at least a portion of the untreated material.5. The assembly of claim 1 , wherein the material from which at least a portion of the article is formed is a substantially non-oxo-biodegradable material.6. The assembly of claim 1 , wherein the at least one agent of oxo-biodegradation comprises a transition metal salt and/or a transition metal chelate.7. The assembly of claim 6 , wherein the transition metal salt is a transition metal stearate selected from the group consisting of chromium stearate claim 6 , cobalt stearate claim 6 , copper stearate claim 6 , iron stearate claim 6 , manganese stearate claim 6 , nickel stearate claim 6 , titanium stearate claim 6 , vanadium stearate claim 6 , and combinations thereof.8. ...

Подробнее
Номер записи: 96
01-05-2014 дата публикации

IMMOBILIZED ENZYME AND ITS FABRICATION METHOD AND REACTION SYSTEM

Номер: US20140120600A1
Автор: CHANG Kuo-Chung, CHENG CHEAN-YEH
Принадлежит: CHUNG YUAN CHRISTIAN UNIVERSITY

This invention provides immobilized enzymes, fabrication methods thereof, and reaction systems using the immobilized enzymes. In an embodiment, silica is silanized first and then modified by nano-gold particles, an amino acid, and a peptide-bond coupling agent in sequence. Finally, the modified silica is chemically bonded with an enzyme. 1. A method for producing an immobilized enzyme , comprising the steps of:providing a silica;activating the silica with an acid solution so as to expose hydroxyl groups on the surface of the silica;{'sub': 3', '3', '2', 'n, 'silanizing the silica by a (mercapto)trimethoxysilane ((CHO)Si—(CH)—SH, where n is an integer);'}modifying the silanized silica by gold nano-particles;modifying the gold nano-particles modified silica by an amino acid with an amino group, a carboxyl group, and a thiol group;modifying the amino acid modified silica on the carboxyl group of the amino acid by a peptide-bond coupling agent with a diimide;chemically bonding an enzyme to the peptide-bond coupling agent modified silica;continuously catalyzing a substrate with the immobilized enzyme; andrecycling and recovering the reacted immobilized enzyme for reusing by filtering, cleaning, drying, and refrigerating, whereby the activity of the recycled and recovered immobilized enzyme is not reduced and is even increased for at least 5 repeated uses by comparing with the activity of the first-time use of the immobilized enzyme.2. The method as recited in claim 1 , wherein the (mercapto)trimethoxysilane ((CHO)Si—(CH)—SH) comprises (3-mercaptopropyl) trimethoxysilane.3. The method as recited in claim 1 , wherein the amino acid comprises L-cysteine.4. The method as recited in claim 1 , wherein the peptide-bond coupling agent comprises N claim 1 ,N′-dicyclohexylcarbodiimide.5. The method as recited in claim 1 , wherein the enzyme comprises cellulase.67-. (canceled)9. The immobilized enzyme as recited in claim 8 , wherein n is 3.10. The immobilized enzyme as recited in ...

Подробнее
Номер записи: 97
11-02-2016 дата публикации

Modified silk films containing glycerol

Номер: US20160038637A1
Автор: David L. Kaplan, Fiorenzo Omenetto, Shenzhou Lu, Xiaoqin Wang
Принадлежит: TUFTS UNIVERSITY

The present invention provides for compositions and methods for preparing aqueous insoluble, ductile, flexible silk fibroin films. The silk films comprise silk fibroin and about 10% to about 50% (w/w) glycerol, and are prepared by entirely aqueous processes. The ductile silk film may be further treated by extracting the glycerol from and re-drying the silk film. Active agents may be embedded in or deposited on the glycerol modified silk film for a variety of medical applications. The films may be shaped into 3-dimentional structures, or placed on support surfaces as labels or coatings. The glycerol modified silk films of the present invention are useful in variety of applications such as tissue engineering, medical devices or implants, drug delivery, and edible pharmaceutical or food labels.

Подробнее
Номер записи: 98
08-02-2018 дата публикации

Structures and methods of manufacturing structures using biological based materials

Номер: US20180037000A1
Автор: Ryan Church
Принадлежит: Individual

A structure for a turbine, the structure comprising a body having a multi-layer construction including an interior layer with substantially uniform concentrations throughout of facultative anaerobic organisms (FAO) that have gene sets capable of producing the enzyme urease and/or the proteins purloin, lustre A and perlustrin, along with glucose, and non-uniform concentrations throughout of a structural composition, the structural composition including a chitin-based component with silk fibronectin and water; an exterior layer of urea, water, calcium ions and facultative anaerobic organisms (FAOs) including urease, aragonite; and a binding layer of conchiolin protein intermediate the interior layer and the exterior layer. The facultative anaeorobic organisms (FAOs) are organisms classified in one of the Saccharomyces, Escherichia and Bacillus genuses.

Подробнее
Номер записи: 99
12-02-2015 дата публикации

Bone Augmentation Utilizing Muscle-Derived Progenitor Compositions in Biocompatible Matrix, and Treatments Thereof

Номер: US20150044185A1
Автор: Huard Johnny, Jankowski Ronald, Payne Karin, Payne Thomas, Usas Arvydas
Принадлежит:

The present invention provides muscle-derived progenitor cells that show long-term survival following transplantation into body tissues and which can augment non-soft tissue following introduction (e.g. via injection, transplantation, or implantation) into a site of non-soft tissue (e.g. bone) when combined with a biocompatible matrix, preferably SIS. The invention further provides methods of using compositions comprising muscle-derived progenitor cells with a biocompatible matrix for the augmentation and bulking of mammalian, including human, bone tissues in the treatment of various functional conditions, including osteoporosis, Paget's Disease, osteogenesis imperfecta, bone fracture, osteomalacia, decrease in bone trabecular strength, decrease in bone cortical strength and decrease in bone density with old age. 134-. (canceled)35. A method of accelerating formation of mineralized matrix on small intestine submucosa (SIS) sheets and/or scaffolds comprising seeding SIS disks with human muscle-derived progenitor cells (hMDCs) in an osteogenic medium to form SIS sheets , wherein the mineralization is achieved within 7 days; wherein the mineralization is accelerated compared to SIS scaffolds not seeded with hMDCs (empty SIS) cultured in the osteogenic medium.36. The method of claim 35 , wherein the hMDCs seeded SIS disks are incubated for 28 days in the osteogenic medium.37. The method of claim 35 , wherein volume of the mineralized matrix is progressively increased as observed at 7 days claim 35 , at 10 days claim 35 , at 14 days claim 35 , at 21 days claim 35 , and at 28 days.38. The method of claim 37 , wherein density of the mineralized matrix in SIS scaffolds with hMDCs cultivated in the osteogenic medium is about 222 mm HA/ccm at 7 days.39. The method of claim 37 , wherein density of the mineralized matrix in SIS scaffolds with hMDCs cultivated in the osteogenic medium is about 200 mm HA/ccm at 28 days.40. The method of claim 35 , wherein the empty SIS scaffolds ...

Подробнее
Номер записи: 100