ЛИПАЗНЫЕ ПОРОШКОВЫЕ СОСТАВЫ

Изобретение относится к области биотехнологии. Предложен порошковый состав, обладающий липазной активностью. Состав содержит фильтрующий вспомогательный материал(ы) и продукт, полученный тонким измельчением липазы, происходящей из Thermomyces sp., иммобилизированной на кремниевом носителе(ях), до среднего диаметра частиц 1 мкм или более до менее чем 300 мкм. Предложены также способы для переэтерификации жиров и масел и для этерификации с использованием полученного липазного порошкового состава. Порошковый состав по данному изобретению обладает улучшенной липазной активностью. 3 н. и 7 з.п. ф-лы, 1 ил., 2 табл.

ОПОСРЕДОВАННАЯ НАНОЧАСТИЦАМИ ДОСТАВКА СИКВЕНС-СПЕЦИФИЧНЫХ НУКЛЕАЗ

Изобретение относится к области биохимии, в частности к способу введения сиквенс-специфичной нуклеазы (ССН) в растительную клетку. При этом способ включает обеспечение наличия растительной клетки, имеющей клеточную стенку; покрытие поверхности наночастицы ССН; приведение растительной клетки, имеющей клеточную стенку, и покрытой наночастицы в контакт друг с другом; и обеспечение поглощения наночастицы и ССН в растительную клетку, содержащую клеточную стенку. Изобретение позволяет эффективно осуществлять введение ССН в растительную клетку, имеющую клеточную стенку. 14 з.п. ф-лы, 7 ил., 5 пр.

Препарат для очистки почв и воды от нефти и нефтепродуктов

Изобретение относится к биотехнологии. Препарат для очистки почв и воды от нефти и нефтепродуктов содержит нефть, биомассу штамма углеводородокисляющих бактерий Bacillus subtilis СНБС- 1, депонированного во Всероссийской Коллекции Промышленных Микроорганизмов под регистрационным номером ВКПМ В-12239, связующий компонент, в качестве которого используют натрийкарбоксиметилцеллюлозу и органоминеральный субстрат-носитель - композиционную смесь на основе клиноптилолита, подготовленных древесных опилок и перегноя в заданном соотношении компонентов. Изобретение позволяет повысить эффективность очистки почвы и воды от нефти и нефтепродуктов. 5 табл., 2 пр.

Гетерогенный катализатор жидкофазного окисления органических соединений

Изобретение относится к химической промышленности, а именно к области производства гетерогенных катализаторов процессов жидкофазного окисления органических соединений (в том числе, производных фенолов) и может быть применено на предприятиях различных отраслей промышленности для проведения реакций окисления, а также для каталитической очистки сточных вод от токсичных органических контаминантов. Гетерогенный катализатор жидкофазного окисления органических соединений содержит носитель, глутаровый диальдегид в качестве сшивающего агента и экстракт корня хрена (Armoracia Rusticana) в качестве активного компонента. Согласно изобретению в качестве носителя используют диоксид титана, модифицированный последовательно 0,095÷0,105 н. раствором соляной кислоты, 0,195÷0,205%-ным раствором хитозана в 0,0045÷0,0055 М растворе соляной кислоты и 4,95÷5,05%-ным раствором аминопропилтриэтоксисилана в 95,5÷96,5%-ном этаноле при следующем соотношении компонентов, % масс.: диоксид титана - 45÷55; хитозан - 7,5 ...

ФИКСИРОВАННЫЙ НА НОСИТЕЛЕ ФЕРМЕНТ И СПОСОБ ЕГО ПОЛУЧЕНИЯ

Изобретение относится к области биотехнологии и может быть применено при ферментативной реакции синтеза, а также для фиксации ферментов на носителе. Фиксированный на носителе фермент выбран из группы, состоящей из пенициллин-G-амидазы (E. C.3.5.I, II), глутарил-7-АСА-ацилазы и оксидазы D-аминокислоты (E.C.I. 4.3.3), ковалентно связан на материале-носителе на основе полиорганосилоксана с функциональными аминогруппами. Способ иммобилизации заключается в ковалентном связывании фермента на материале-носителе с помощью диальдегида. В каждом конкретном случае устанавливают оптимальное соотношение фермента к материалу-носителю. Используют материал-носитель со средним диаметром частиц, преимущественно 0,01-3 и 0,1-0,4 мм, а также шарообразный. Предложенный фиксированный на носителе фермент обладает повышенной объемной активностью и стабильностью. 2 с. и 19 з.п. ф-лы, 2 табл., 5 ил.

УСТРОЙСТВО ДЛЯ БИООБРАСТАНИЯ БИОПЛЕНКИ В ПРОСВЕТЕ ТРУБОПРОВОДОВ И ВОЗДЕЙСТВИЯ ДЕЗИНФИЦИРУЮЩИХ СРЕДСТВ НА БИОПЛЕНКИ В ПРОТОЧНЫХ СИСТЕМАХ

Полезная модель относится к современным приборам для изучения биопленок, предназначена для микроскопического (визуального) исследования жизненного цикла биопленок, образованных различными сообществами микроорганизмов на различных материалах и в различных средах в условиях контролируемого воздействия температуры, освещения, скорости перемещения жидкой среды, состава газовой фазы.Устройство для изучения процесса воздействия дезинфицирующих средств на биопленки в проточных системах, включающее открытый или закрытый резервуар, соединенный системой трубопроводов циркуляционный насос, систему изолированных двумя кранами трубопроводов и запорный кран. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 179 657 U1 (51) МПК C12Q 1/24 (2006.01) C12N 11/14 (2006.01) C12M 3/04 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ (52) СПК C12Q 1/24 (2006.01); C12N 11/14 (2006.01); C12M 3/04 (2006.01) (21)(22) Заявка: 2017130117, 25.08.2017 (24) Дата начала отсчета срока действия патента: Дата регистрации: 21.05.2018 (45) Опубликовано: 21.05.2018 Бюл. № 15 1 7 9 6 5 7 R U (56) Список документов, цитированных в отчете о поиске: RU 2559546, 10.08.2015. РОМАНОВА Ю.М., АЛЕКСЕЕВА Н.В. и др., Способность к формированию биопленок в искусственных системах у различных штаммов Salmonella Typhimurium // Журнал микробиологии, эпидемиологии и иммунологии, 4, 2006, стр. 38-42. RU 2477320 C2, 10.03.2013. US 5,281,537, 25.01.1994. US 3510406 A, 05.05.1970. US 3510406 A, 05.05.1970. (54) УСТРОЙСТВО ДЛЯ БИООБРАСТАНИЯ БИОПЛЕНКИ В ПРОСВЕТЕ ТРУБОПРОВОДОВ И ВОЗДЕЙСТВИЯ ДЕЗИНФИЦИРУЮЩИХ СРЕДСТВ НА БИОПЛЕНКИ В ПРОТОЧНЫХ СИСТЕМАХ (57) Реферат: Полезная модель относится к современным фазы. приборам для изучения биопленок, предназначена Устройство для изучения процесса воздействия для микроскопического (визуального) дезинфицирующих средств на биопленки в исследования жизненного цикла биопленок, проточных системах, включающее открытый или образованных различными ...

СОРБЕНТ ДЛЯ ОЧИСТКИ ПРИРОДНЫХ ВОД И ПОЧВЫ ОТ НЕФТЯНЫХ ЗАГРЯЗНЕНИЙ "МОСКАТ"

Изобретение относится к экологии. Предложен сорбент для очистки экосистем от нефтепродуктов, содержащий алюмосиликатный носитель с иммобилизованными микроорганизмами RHODOCOCCUS SP. штамм 30 и фосфор- и азотсодержащие соли. Сорбент обеспечивает высокую степень очистки при полной экологической безопасности. 1 з.п. ф-лы, 3 табл.

Гетерогенный катализатор жидкофазного окисления органических соединений и способ его получения

Изобретение относится к химической промышленности, а именно к области производства гетерогенных катализаторов процессов жидкофазного окисления органических соединений (в том числе производных фенолов), и может быть применено на предприятиях различных отраслей промышленности для проведения реакций окисления, а также для каталитической очистки сточных вод от токсичных органических загрязнителей. Гетерогенный катализатор жидкофазного окисления органических соединений содержит носитель, модифицированный 3-аминопропилтриэтоксисиланом, глутаровый диальдегид в качестве сшивающего агента и пероксидазу корня хрена в качестве активного компонента, в котором носителем являются магнитные наночастицы Fe3O4, модифицированные SiO2, при следующем соотношении компонентов, % мас.: Fe3O4- 34,2÷34,6; SiO2- 41,0÷41,4; 3-аминопропилтриэтоксисилан - 18,3÷18,8; глутаровый диальдегид - 3,8÷4,0; пероксидаза хрена - 1,9÷2,0. Способ получения гетерогенного катализатора жидкофазного окисления органических соединений ...

ОПОСРЕДОВАННАЯ НАНОЧАСТИЦАМИ ДОСТАВКА СИКВЕНС-СПЕЦИФИЧНЫХ НУКЛЕАЗ

... 1. Способ введения сиквенс-специфичной нуклеазы (ССН) в растительную клетку, где способ включает:обеспечение наличия растительной клетки, имеющей клеточную стенку;покрытие поверхности наночастицы ССН с помощью подхода, выбранного из группы,состоящей из электростатической адсорбции, конъюгации с лигандом на поверхности наночастицы, покрытия наночастицы малым кофактором, который ССН может распознать и связать, и непосредственной конъюгации с наночастицей;приведение растительной клетки, имеющей клеточную стенку, и покрытой наночастицы в контакт друг с другом; иобеспечение поглощения наночастицы и ССН в растительную клетку, содержащую клеточную стенку.2. Способ по п. 1, в котором покрытие наночастицы ССН включает иммобилизацию ССН за счет нековалентной адсорбции на поверхности наночастицы.3. Способ по п. 1, который дополнительно включает поглощение ССН внутрь наночастицы.4. Способ по п. 1, который дополнительно включает обеспечение поглощения наночастицы компартментом растительной клетки, имеющей ...

НЕБОЛЬШИЕ ФЕРМЕНТО-СОДЕРЖАЩИЕ ГРАНУЛЫ

... 1. Совокупность ферменто-содержащих гранул, где, по меньшей мере, около 95% указанных гранул содержит одно ядро и ферменто-содержащий слой, нанесенный поверх указанного ядра, причем указанное ядро состоит из одной или нескольких неорганических солей, и где, по меньшей мере, около 80% указанных гранул имеет диаметр около 150-355 мкм. ! 2. Совокупность гранул по п. 1, где указанное ядро состоит из сульфата натрия. ! 3. Совокупность гранул по п. 2, где, по меньшей мере, около 80% указанных натриево-сульфатных ядер имеет диаметр около 100-250 мкм. ! 4. Совокупность гранул по п. 1, где ферменто-содержащий слой дополнительно содержит, по меньшей мере, один из компонентов: полимер, сахар, крахмал или поверхностно-активное вещество. ! 5. Совокупность гранул по п. 1, дополнительно содержащая нанесенный поверх указанного ферменто-содержащего слоя слой, содержащий барьерную соль. ! 6. Совокупность гранул по п. 5, где барьерный солевой слой содержит сульфат натрия. ! 7. Совокупность гранул по п. 5, ...

СПОСОБЫ И МИКРООРГАНИЗМЫ ДЛЯ ПОЛУЧЕНИЯ 1,4-БУТАНДИОЛА И ЕГО ПРОИЗВОДНЫХ ИЗ С1-УГЛЕРОДНЫХ СОЕДИНЕНИЙ

Магнитоотделяемый катализатор окисления органических соединений и способ его получения

Настоящее изобретение относится к гетерогенному катализатору жидкофазного окисления глюкозы и технологии его получения и может применяться на предприятиях химической и фармацевтической промышленности для получения компонентов пищевых продуктов и биологически активных добавок, таких как глюконовая кислота и глюконат кальция. Магнитоотделяемый катализатор окисления глюкозы, содержащий в качестве носителя магнитные наночастицы Fe3O4, модифицированные хитозаном и триполифосфатом натрия, и глюкозооксидазу из Aspergillus niger, характеризуется тем, что носитель дополнительно включает ацетилцистеин. Соотношение компонентов катализатора в % по массе составляет: Fe3O4 - 82,65÷83,22, хитозан - 8,25÷8,35, триполифосфат натрия - 1,61÷1,71, ацетилцистеин - 0,80÷0,86, глюкозооксидаза - 6,12÷6,32. Технический результат изобретения заключается в повышении активности, селективности и стабильности катализатора в реакции окисления глюкозы, а также его способности к отделению от реакционной среды. 2 н.п. ф-лы ...

BIOPROCESSING

BIOPROCESSING

Bioprocessing.

Bioprocessing.

BIOPROCESSING

Bioprocessing.

BIOPROCESSING

PROCEDURE FOR THE PRODUCTION OF HEATPROOF ENZYMES

PROCEDURE AND DEVICE FOR THE MEASUREMENT OF THE CONCENTRATION OF LOWMOLECULAR EN CONNECTIONS IN COMPLEXES MEDIA

Process for the immobilization of proteins, peptides, coenzymes, ligands on a support

In a process for the immobilization of proteins, peptides, coenzymes and ligands on a support which has amino, mercapto or hydroxyl groups, a support is reacted with a compound of the general formula I in which X is halogen, and R1, R2 are identical or different and are X, R, COR, COOH, COOR, CN, CNS, N3, in which R is C1-C8- alkyl, and dried if required, where one of the X substituents reacts with an amino, mercapto or hydroxyl group of the support material, after which the proteins, peptides, coenzymes, ligands and redox mediators to be immobilized are reacted with the second X substituent which is adjacent to the X substituent which has already reacted and is para to a C=O group. ...

Process for the immobilization of proteins, peptides, coenzymes on a support

In a process for the immobilization of proteins, peptides, coenzymes on a support which has amino, mercapto or hydroxyl groups, a support is reacted with a compound of the general formula I in which X is halogen, and R1, R2 are identical or different and are X, COOH, CN, R, COR, COOR, in which R is C1-C8-alkyl, and dried if required, after which the peptides, proteins, coenzymes to be immobilized are reacted with the free halogen in the para-position of the compound of the general formula I. ...

IMMOBILISIERTES BETA-GALACTOSIDASE-ENZYM- PRAEPARAT UND VERFAHREN ZU SEINER HERSTELLUNG

VERFAHREN ZUM IMMOBILISIEREN VON PROTEINEN, PEPTIDEN, COENZYMEN, LIGANDEN AN EINEM TRAEGER

PROCEDURE FOR THE CONNECTION OF ENZYME TO A CARRIER USING KATIONI COPOLYMERS AND THEREBY MANUFACTURED PRODUCT

ENZYME PREPARATIONS

PROCEDURE FOR THE IMMOBILIZATION OF PROTEINS, PEPTIDEN, COENZYMEN AT A CARRIER

AIR CLEANING FILTER AND PROCEDURE FOR THE PRODUCTION OF THE SAME

fILTER SUBSTRATE FOR BIOLOGICAL MARKET PREPARATION CONTRACTS OF pURE WATER AND METHODS FOR PREPARATION

Die Erfindung betrifft ein Filtersubstrat zur biologischen Aufbereitung von Reinwasser aus künstlich hergestellten Substratteilchen (7). Die Oberfläche der Substratteilchen (7) ist mit bestimmten, vorab definierten, im aufzubereitenden Reinwasser in Mangel vorhandenen Elementen für den Biofilmaufbau in biologisch verfügbarer Form konditioniert worden, wobei die Substratteilchen (7) von jenem Element bzw. jenen Elementen, welche anwendungsspezifisch das bzw. die zu limitierende(n) Element(e) im aufzubereitenden Reinwasser ist bzw. sind, in biologisch verfügbarer Form frei ist bzw. sind.

MUTATED PENICILLIN G ACYLASEN

RESUSPENDIERBARE COATED MAGNETIC PARTICLES AND STABLE ONE MAGNETIC PARTICLE SUSPENSIONS

CARRIER FOR BIOLOGICALLY EFFECTIVE MOLECULES OR BIOLOGICAL OF EFFECTIVE CARRIERS, PROCEDURES FOR ITS PRODUCTION AND ITS BIOLOGICAL USE.

AMORPHOUS SILICIC ACID PARTICLES, PROCEDURES FOR THEIR PRODUCTION AND THEIR USE.

TONHALTIGES MATERIAL AND PROCEDURE FOR THE PRODUCTION OF THE SAME.

PROCEDURE FOR CHEMICAL IMMOBILISATION BIOLOGICALLY ACTIVE SUBSTANCES ON SILICA.

PROCEDURE FOR SPLITTING OF PRO ENZYMES

PROCEDURE FOR THE PRODUCTION OF IMMOBILIZED LIPASEN

ENDOWED SOL GEL GLASSES FOR CHEMICAL INTERACTIONS

CARRIER-FIXED PENICILLIN G AMIDASE, GLUTARYL-7 ACA ACYLASE OR D-AMINOSAÜRE-OXIDASE

PROCEDURE FOR THE IMMOBILIZATION OF BIOLOGICAL MATERIAL ON A DOCUMENT

PROCEDURE FOR THE CONNECTION OF BIOLOGICAL CATALYTIC ACTIVITIES TO SINTERED EXPANDED ONES CLAY/TONE AND THEREBY RECEIVED PRODUCTS

IMMOBILIZED ENZYME AND PROCEDURE FOR THE PRODUCTION OF OPTICALLY ACTIVE ONE CYANHYDRINEN

Procedure for the production of Deacylaseenzymzubereitungen

SPRAY-DRIED ENZYME PRODUCT

VERFAHREN ZUR SPALTUNG VON PROTEINEN

PROCEDURE FOR THE PRODUCTION OF IMMOBILIZED ENZYMKONJUGATEN AND SO PRESERVATION IMMOBILIZED ENZYMKONJUGATE

PROCEDURE FOR THE PRODUCTION OF AN IMMOBILIZED ENZYME PREPARATION AND YOUR USE

LIPASE ON SOLID PHASE

Method for transforming arsenic sulfide slag and curing and stabilizing resulting compound by means of microencapsulation

The present invention provides a method for transforming an arsenic sulfide slag and curing and stabilizing the resulting compound by means of microencapsulation, comprising the following steps: (1) preparing arsenic trioxide from the arsenic sulfide slag as a raw material; (2) preparing 4-hydroxy-3-nitrophenylarsonic acid from the arsenic trioxide as a raw material; (3) preparing an iron-manganese dinuclear cluster metal arsenate compound having a porous structure; (4) subjecting the iron-manganese dinuclear cluster metal arsenate compound having a porous structure to surface coating with silicon; (5) synthesizing a Fe(0)/Al-SBA-15 mesoporous composite stabilizer by a hydrothermal reaction; and (6) subjecting the silicon coated iron-manganese dinuclear cluster metal arsenate compound to curing and stabilizing treatment by means of microencapsulation. The present invention involves transforming the arsenic sulfide slag into 4-hydroxy-3-nitrophenylarsonic acid and finally into a metal arsenate ...

Immobilized enzyme complexes and related methods

Immobilized enzyme complexes (IEC) with enzymes that are non-covalently linked to matrices are provided along with methods for making the same. Methods of using the IEC for a wide variety of industrial enzymatic processes are also provided. Methods of converting cellulosic biomass and methods of effecting blood type conversions with the IEC are amongst the methods disclosed.

APATITE IMMOBILIZED GLUCANASE

Enzyme granulate

GLASS FIBER MAT

Materials and method for immobilizing, isolating, and concentrating cells using carboxylated surfaces

The present disclosure relates to immobilization of a cell using a carboxylated surface by contacting the carboxylated surface with a sample comprising the cell for a sufficient time to permit the cell to bind to the carboxylated surface. The immobilized cell may then be separated from the remainder of the sample and further manipulated to isolate, concentrate, and/or analyze the cell or a component thereof.

Capsules with porous mineral cortex

COLUMN FOR A CIRCULATING DIALYSATE SYSTEM

LIPASE AND OTHER PROTEINS PHYSICALLY BOUND TO A SOLID SUPPORT

GLUCOAMYLASE IMMOBILIZED ON CATIONIC COLLOIDAL SILICA

Support for enzymes and proteins comprising an inorganic solid and combined material comprising the support

Process and reaction particles for carrying out reactions

NON-VITREOUS, NON-FUSED, MONOLITHIC CERAMIC FIBRES WITH POROUS OUTER LAYER AND NON-POROUS CORE

METHOD USING GLUCOAMYLASE IMMOBILIZED ON POROUS ALUMINA

METHOD FOR PREPARING A SUBSTRATE FOR IMMOBILISING A CELL, SAID SUBSTRATE AND USES THEREOF

La présente invention concerne un procédé de préparation d'un support sol ide susceptible d'immobiliser au moins une cellule et/ou au moins une partie de cellule, ledit procédé comprenant une étape consistant à fixer audit sup port solide un composé fusogène capable de s'insérer dans les membranes cell ulaires. La présente invention concerne également un procédé pour immobilise r au moins une cellule et/ou au moins une partie de cellule utilisant le sup port solide ainsi préparé, ledit support solide et ses utilisations dans le domaine du diagnostic biomédical ou de la surveillance sanitaire de fluides biologiques ou destinés à l'usage humain ou animal.

CERAMIC ARTICLES HAVING A NONPOROUS CORE AND POROUS OUTER LAYER

This invention relates to high technology ceramics. The ceramic articles of this invention are fired, non-vitreous, monolithic, non-fused ceramic articles in various forms. Articles capable of being filled or infiltrated with certain substances in a controlled geometry and in controlled amounts to render the articles useful for special applications, e.g., catalytic, reinforcing, are rare. The articles of this invention exhibit a uniform, porous sheath and a core. The porous sheath or outer layer of the articles can be filled with various infiltrates, e.g., catalysts, in order to provide the articles with useful properties. The articles can be prepared by treating fired ceramic articles with a leachant, e.g, hydrofluoric acid or a precursor thereof.

AMINOORGANIC TYPE MINERAL SUPPORTS FOR THE FIXATION OF ENZYMES

Complexes support-enzyme constitués d'enzymes fixées par liaison covalente à des supports minéraux greffés et caractérisés en ce que les enzymes sont liées à au moins une fonction réactive de groupements organiques choisis dans le groupe constitué par des restes aliphatiques linéaires ou ramifiés; dont la chaîne possède de 1 à 8 atomes de carbone, des restes cyclaniques, des restes aryles et des restes alkylaryles. Ces complexes sont obtenus par réaction d'un support minéral insoluble possédant des groupes hydroxyle avec un composé formé d'un groupement organique possédant au moins une fonction alcool ou phénol et au moins une fonction réactive, à laquelle l'enzyme est ensuite liée. Les complexes support-enzyme selon l'invention sont stables et résistants aux facteurs de dénaturation, pH, température et peuvent être utilisés aussi bien en discontinu qu'en continu, sans perdre leur activité enzymatique. Ils peuvent être employés en chromatographie d'affinité, catalyse enzymatique, analytique ...

PROTEIN IMMOBILIZER

Biologically active proteins are immobilized on polar supports by first applying to the support a monolayer of a water-soluble polymer containing a .beta.-hydroxyalkylene-amine moiety wherein the equivalent weight of the polymer based on the .beta.-hydroxyalkyleneamine moiety is 87 to about 10,000. The protein irreversibly attaches to the polymertreated support and retains substantially all of its biological activity.

METHOD AND AN ARRANGEMENT FOR THE MEASURING OF THE CONCENTRATION OF LOW-MOLECULAR COMPOUNDS IN COMPLEX MEDIA ICULARLY IN MEDICAL TREATMENT

The present invention provides a method for the measurement of the content of a low-molecular compound in a complex medium, in which a small portion of the complex medium is dialysed via a semipermeable membrane whereupon the measurement of the dialysate is carried out. The invention also provides a method for the measurement of any low-molecular compound the dialysate is diluted before a first enzyme bed and/or before a second enzyme bed with water, buffer and/or reagent solution.

SUPPORT MATRIX FOR AMINO-CONTAINING BIOLOGICALLY ACTIVE SUBSTANCES

An improved immobilized enzyme composite is prepared by subjecting a siliceous carrier to an initial treatment with alkali, then acid, then reacting the carrier with an organosilane. The organosilane may then be coupled through a covalent coupling agent with an enzyme. The alkali-acid treatment provide n exceptionally high number of hydroxyl groups per unit volume. so that the treated carrier is especially useful in the preparation of high performance immobilized enzyme compositions, particularly by multi-layering immobilization, providing a very high amount of enzyme activity per unit volume. Multi-layering is accomplished by successive steps of bonding a difunctional covalent coupling agent to a previously immobilized enzyme layer, then bonding an additional layer of enzyme to the coupling agent. This may be repeated to apply as many layers as desired.

PROCESSES FOR PREPARING IMMOBILIZED ENZYME AND REACTION PRODUCT WITH SAID IMMOBILIZED ENZYME

The present invention relates to processes for preparing immobilized enzyme, particularly immobilized-starch-degrading enzyme, and reaction product with said immobilized enzyme. More precisely, the present processes are based on the invention that an immobilized-starch-degrading enzyme having a high starch-degrading activity is easily obtainable by modifying in solution a starch-degrading enzyme with a modifying reagent in a manner that the enzyme is not insolubilized substantially, and adsorbing the modified enzyme onto a carrier. The processes are also based on the finding that reaction products from amylaceous materials can be easily produced with said immobilizedstarch-degrading enzyme.

IMMOBILIZED ENZYMES, A PROCESS FOR THEIR PREPARATION, AND THEIR USE IN CONVERTING SUBSTRATES TO PRODUCTS

Immobilized enzyme products wherein active immobilized enzyme is part of an external gel or other layer on the support material can be made by a process which comprises forming a gellable, external, enzyme-containing coating on support material by contacting the inert support material with an aqueous solution of the enzyme to be immobilized and with a water-miscible organic solvent, and contacting the externally coated support material with a cross-linking agent to gel the coating.

PROCESS FOR THE PRODUCTION AND IMMOBILIZATION OF MODIFIED PROTEINS

PRINTABLE MAGNETIC POWDERS AND 3D PRINTED OBJECTS FOR BIONANOCATALYST IMMOBILIZATION

The invention provides materials, and in particular, magnetic materials, for the universal immobilization of enzymes and enzyme systems. Described herein are highly magnetic and highly porous composite blends of thermoplastics with magnetic particles to form powders, single-layered, or multiple-layered materials that are used as scaffolds for magnetically immobilized enzymes known as bionanocatalysts (BNCs). Designed objects are produced using 3D printing by sintering composite magnetic powders. In some embodiments, Selective Laser Sintering (SLS) is used. The invention provides the use of the material compositions for 3D printing of enzyme supports and flow cells allowing continuous production of, e.g., small molecules.

BIOCHARS FOR USE WITH ANIMALS

Biochars and methods for treating biochars are provided that are useful in various applications, including, but not limited to, applications related to the raising, care, maintenance, disease prevention, disease treatment and odor control of animals.

REACTION METHOD, REACTION APPARATUS AND ENZYME

A reaction method wherein a mediator in an activated state is poured into a reaction system containing a substrate. In this method, it is preferable to activate the mediator under mild conditions with the use of an enzyme as a preliminary step. It is particularly preferable that a highly active mediator, which has been improved in the activity and/or stability, is poured into the above-described reaction system. A method of enzymatically decomposing a substrate comprising immobilizing an oxidizing enzyme peroxidase in a structural unit which has almost the same size as the enzyme size and a structural stability, and decomposing the substrate in the presence of an oxidizing agent at such a concentration as being accepted by the immobilization. A reaction promoter which is to be used in a reaction system of decomposing a substrate with an oxidizing enzyme, has a diketone structure allowing radicalization due to the function of the oxidizing enzyme and is soluble in water. A manganese peroxidase ...

METHOD OF PRODUCING NANOBIOCATALYSTS

A method of producing nanobiocatalysts comprises: suspending a support material in a liquid medium; functionalizing a surface of the support material by mixing the suspended support material with a functionalizer for a first predefined time at a first predefined temperature wherein a first broth results; applying a protein directly in the first broth after the first predefined time; mixing the protein with the first broth and incubating the protein for a second predefined time at a second predefined temperature wherein a second broth results; immobilizing the incubated protein onto the functionalized surface of the support material wherein a third broth results; filtering the third broth; and gathering a retentate of the filtering. By directly processing the first and second broths the method according to the invention allows for providing a continuous process and production of the nanobiocatalysts. Thereby, the production can be optimized regarding time, water, chemical and energy consumption ...

MATERIALS AND METHODS FOR PRODUCING CELL-SURFACE DIRECTED AND ASSOCIATED NON-NATURALLY OCCURRING BIOINORGANIC MEMBRANES AND USES THEREOF

Materials and methods are provided for producing cell-surface directed, non-naturally occurring, bioinorganic membranes for association with the cell surfaces of living cells. The methods comprise exposing a cell to an acidic biomineralization buffer environment for cell-mediated deposition of the biomineral membrane onto the surface of the cell. The methods also comprise attaching a peptide, having a net positive charge under the acidic conditions, to the cell surface for serving as a template in directing the cell-mediated deposition of the biomineral membrane onto the surface of the cell.

IMMOBILIZED BIOCATALYST, ITS PREPARATION AND USE FOR ESTER SYNTHESIS IN A COLUMN REACTOR

HOE 91/F 100 of the disclosures An immobilized biocatalyst, its preparation and use for ester synthesis in a column reactor The invention relates to a process for the acylation of alcohols, with the exception of tertiary alcohols, using lipases from Pseudomonas, Mucor, Candida, Rhizopus, Penicillium or pig pancreas, which are immobilized on inert carriers (seasand, powdered rock, powdered brick, glass, ceramic powders).

ENZYME CONTAINING COATED GRANULES

A granular enzyme composition is disclosed, having reduced tendencies to form dust and leave residue, and exhibiting improved stability and delayed release characteristics. The granular composition comprises a core, an enzyme layer and an outer coating layer. The enzyme layer, and optimally the core and coating layers, contain a vinyl polymer. Also disclosed are methods for making such enzyme-containing granules, the methods having greatly reduced processing time.

SILICEOUS SUPPORT MEDIA FOR IMMOBILIZATION OF ENZYMES

A simple, efficient method for immobilization of enzymes on siliceous supports is reported. The support and the enzyme are linked using a polyfunctional cross-linking agent such as glutaraldehyde. Owing to properties peculiar to siliceous supports, the resulting immobilized enzyme is much more stable than enzymes immobilized on other supports. Consequently, much less enzyme is required. The immobilized enzymes generated via this process are ideally suited for continuous enzymatic production of commodity chemicals and pharmaceuticals such as L-DOPA, and for other enzyme-mediated industrial processes.

SILICEOUS SUPPORT MEDIA FOR IMMOBILIZATION OF ENZYMES

A simple, efficient method for immobilization of enzymes on siliceous supports is reported. The support and the enzyme are linked using a polyfunctional cross-linking agent such as glutaraldehyde. Owing to properties peculiar to siliceous supports, the resulting immobilized enzyme is much more stable than enzymes immobilized on other supports. Consequently, much less enzyme is required. The immobilized enzymes generated via this process are ideally suited for continuous enzymatic production of commodity chemicals and pharmaceuticals such as L-DOPA, and for other enzyme-mediated industrial processes.

Procédé de préparation d'un produit doué d'activité enzymatique, insoluble en milieu aqueux

Procédé pour l'obtention d'articles textiles porteurs d'enzymes

PROCEDE DE FABRICATION D'UNE PREPARATION ENZYMATIQUE INSOLUBLE DANS L'EAU.

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Immobilised, support-fixed, enzyme

Hesperetin dihydrochalcone glucoside sweetener prepn. - by hesperidin dihydrochalcone degradation using carrier-bonded glycolytic enzyme system

Hesperetine dihydrochalcone glucoside, used as sweetener, is prepd. from hesperidin dihydrochalcone by using a carrier-bonded enzyme or enzyme system, pref. hesperidinase, having inhibited beta-glucosidase activity. Fixation to a carrier selectively inhibits beta-glucosidase activity without loss in rhamnosidase activity. Carrier may be an (in)org. polymer, e.g. cellulose, polyamides, but pref. activated silicate-contg. materials, esp. porous glass which is activated with a layer of diazotised aromatic diamine, or 4,4'-diaminodiphenylmethane.

1,3-Phenylenediamine/glutaraldehyde condensation product - useful as substrate for immobilizing proteins, esp. enzymes

A condensation prod. obtd. by reaction of 1,3-phenylenediamine with glutaraldehyde is of use as a substrate for immobilizing proteins e.g. enzymes; it is formed in large particles, and has a high protein-binding capacity. The immobilized proteins (esp. enzymes) can be used for a variety of purposes, e.g. for analytical or preparative purposes or in food technology (e.g. in the prodn. of aromas). Pref. the condensation is carried out in the presence of a finely ground, solid inert additive spec. silica gel, and pref. the reaction is a two-phase system consisting of water and CHCl3.

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PROCEDURE FOR THE ADJUSTMENT OF A BIOLOGICALLY ACTIVE SUBSTANCE ON A POROUS SUBSTRATE.

PROCEDURE AND DEVICE FOR THE MEASUREMENT OF THE CONCENTRATION OF LOWMOLECULAR CONNECTIONS IN COMPLEXES MEDIA.

Condensn prods of aromatic polyamines with dialdehydes or acrolein

Condensn prods of aromatic polyamines with dialdehydes or acrolein useful for fixing proteins, esp enzymes ...

Hydrophobic magnetic particles

A process for making a particulate material comprising mesoporous particles having granules of a metal containing species in at least some of the pores thereof, said process comprising: allowing a compound of the metal to enter pores of hydrophobic mesoporous particles, said compound being thermally decomposable at a decomposition temperature to form a metal containing species and said particles being substantially thermally stable at said decomposition temperature; and heating the hydrophobic mesoporous particles having the compound in the pores thereof to the decomposition temperature so as to decompose the compound and to form the mesoporous particles having granules of the metal containing species in at least some of the pores thereof.

Cell concentration, capture and lysis devices and methods of use thereof

The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Heterogenous enzymatic catalyst, preparation method, and use

The invention relates to a heterogenous enzymatic catalyst that takes the form of a cellular monolith consisting of a silica or organically modified silica matrix, said monolith including macropores, mesopores, and micropores, said pores being interconnected, and wherein the inner surface of the macropores is functionalized by a coupling agent selected from among silanes, said inner surface moreover having an unpurified enzyme attached thereon by means of a covalent or electrostatic bond. The invention also relates to the method for preparing said catalyst, said method comprising: a first step for preparing a solid silica impression that takes the form of a cellular monolith such as defined above; a second step for functionalizing the inner surface of the macropores via a coupling agent, selected from among silanes, by vacuum-soaking the cellular monolith by dissolving the coupling agent in an organic solvent; and a third step for vacuum-soaking the thus-functionalized monolith by means of an aqueous solution or aqueous dispersion of at least one unpurified enzyme. Finally, the invention relates to the use of such catalyst to carry out catalyzed chemical reactions.

Materials and methods for producing cell-surface directed and associated non-naturally occurring bioinorganic membrances and uses thereof

Materials and methods are provided for producing cell-surface directed, non-naturally occurring, bioinorganic membranes for association with the cell surfaces of living cells. The methods comprise exposing a cell to an acidic biomineralization buffer environment for cell-mediated deposition of the biomineral membrane onto the surface of the cell. The methods also comprise attaching a peptide, having a net positive charge under the acidic conditions, to the cell surface for serving as a template in directing the cell-mediated deposition of the biomineral membrane onto the surface of the cell.

METHOD OF REGENERATING AN ENZYMATI CATALYST

A method of regenerating an enzymatic catalyst arranged in a reactor includes a mineral support based on metal oxide and at least one enzyme, wherein it contains at least one step of detachment of the spent enzymes by solvation by scavenging of the catalyst using at least one ionic surfactant, and at least one step of re-attachment of active enzymes by scavenging of the purified support with at least one solution of active enzymes, the two steps being performed in situ within the reactor. 1. A method of regenerating an enzymatic catalyst arranged in a reactor comprising a mineral support based on at least one metal oxide and at least one enzyme , at least one step of detachment of the enzymes by solvation by scavenging of the catalyst using at least one ionic surfactant , and at least one step of re-attachment of active enzymes by scavenging of the purified support with at least one solution of active enzymes , these two steps being performed in situ within the reactor.2. The method according to claim 1 , wherein the step of detachment of the enzyme comprises scavenging of the catalyst with an aqueous solution of so-called amphiphilic ionic surfactant selected from the group including salts of alkyl sulphonates claim 1 , salts of alkyl sulphates claim 1 , salts of alkyl sulphosuccinates claim 1 , salts of alkyl phosphate esters claim 1 , salts of alkylbenzene sulphonates claim 1 , and quaternary ammonium salts.3. The method according to claim 1 , wherein the concentration of spent enzymes measured by absorbance at a wavelength characteristic of the enzyme in UV spectrometry decreases in the outgoing effluent over the entire duration of the scavenging.4. The method according to claim 1 , wherein the end of the detachment step is reached when the differential measurement of the concentration of enzymes expressed by its absorbance between the outgoing effluent and the stream entering the reactor becomes zero.5. The method according to claim 1 , wherein among the ionic ...

Particle matrix for storage of biomolecules

Matrices for manipulation of biopolymers, including the separation, purification, immobilization and archival storage of biopolymers is disclosed.

Support for protein immobilization, immobilized protein, and methods for producing the same

A support for enzyme immobilization is described, which is for immobilizing enzymes of various molecular sizes and also for, due to the modification of the surface silanol groups of porous silica particles, for immobilizing various kinds of enzymes, and enables the design of an immobilized enzyme, which exhibits an activity equivalent to that of the corresponding non-immobilized enzyme and withstands repeated use. A method for producing the support is also described. The support includes porous silica particles having an interparticle void structure therein, characterized in that the porous silica particles have a specific average particle size, a specific surface area, a specific pore volume, a specific pore size distribution and a specific porosity and have a substituent containing an organic group or an amino group on the surface thereof. An immobilized protein obtained by immobilizing a protein on the above support is also described.

Selective photo-induced protein immobilization using bovine serum albumin

Provided is a biomaterial immobilizing method including immobilizing a bovine serum albumin on a substrate, providing a biomaterial on the substrate immobilized with the bovine serum albumin, and irradiating an ultraviolet light onto the substrate provided with the bovine serum albumin and the biomaterial to immobilize the biomaterial selectively on the substrate immobilized with the bovine serum albumin.

Phospholipase-Carrier Complex

A phospholipase-phospholipase-carrier complex for degumming crude oils. The phospholipase-carrier complex includes at least one phospholipase A1, A2 or B and at least one carrier, such as silicic acid, precipitated silicic acid, polyacrylate, polymethacrylate or divinylbenzene-crosslinked methacrylate. The disclosure further relates to a method of degumming crude oils and to a method of producing the phospholipase-carrier complex. 1. A phospholipase-carrier complex comprising at least one phospholipase A1 , A2 or B or a mixture thereof and at least one carrier for degumming crude oils.2. The phospholipase-carrier complex according to claim 1 , wherein the carrier has a pore volume according to BJH of more than 0.1 ml/g for pores with a diameter of from 1.7 to 300 nm.3. The phospholipase-carrier complex according to claim 1 , wherein the carrier has a pore diameter according to BJH of more than 2 nm.4. The phospholipase-carrier complex according to claim 1 , wherein the carrier is selected from silicates and organic polymers or copolymers.5. The phospholipase-carrier complex according to claim 4 , wherein the silicate is selected from the group consisting of natural and synthetic silicates and layered silicates or a mixture thereof.6. The phospholipase-carrier complex according to claim 5 , wherein the silicate is based on silicon dioxide.7. The phospholipase-carrier complex according to claim 4 , wherein the organic polymer or copolymer is selected from the group consisting of polyacrylate claim 4 , polymethacrylate claim 4 , polyethylene claim 4 , polyethylene terephthalate claim 4 , polytetrafluorethylene claim 4 , polypropylene claim 4 , polyvinyl styrene claim 4 , polystyrene claim 4 , styrene-divinylbenzene copolymers claim 4 , polymethylmethacrylate claim 4 , and polyamide or a mixture thereof.8. The phospholipase-carrier complex according to claim 6 , wherein the silicate is silicic acid claim 6 , precipitated silicic acid or a mixture thereof.9. The ...

HETEROGENEOUS ENZYMATIC CATALYST, PROCESS FOR PREPARING SAME AND USE FOR CONTINUOUS FLOW ENZYMATIC CATALYSIS

The present invention relates to a heterogeneous enzymatic catalyst consisting of a macroprous silica monolith incorporating an enzyme immobilized by means of a compiling agent, to a process for preparing this enzymatic catalyst, to the use of the catalyst for carrying out chemical reactions by continuous flow heterogeneous enzymatic catalyst and to a process of continuous flow heterogeneous enzymatic catalysis using said catalyst. 1. A heterogeneous enzymatic catalyst , in the form of a cellular monolith comprising:{'sub': A', 'E, 'a silica monolith, said monolith being free of micropores and having macropores having a mean size dof from 1 μm to 100 μm and mesopores having a mean size dof from 2 to 50 nm, said macropores being interconnected, and in which the internal surface of the macropores is functionalized with a coupling agent, chosen from silanes, to which an enzyme is attached by means of either one of a covalent or electrostatic bond.'}2. The catalyst as claimed in claim 1 , wherein the enzyme immobilized is an unpurified enzyme.3. The catalyst as claimed in claim 1 , wherein the macropores have a mean size dranging from 10 to 100 μm.4. The catalyst as claimed in claim 1 , wherein said monolith has a specific surface area of from 200 to 1000 m/g.5. The catalyst as claimed in claim 1 , wherein the coupling agent is chosen from silanes selected from the group consisting of γ-glycidoxypropyltrimethoxysilane; silylated ionic liquids and silanes of formula Si(OR)Rin which Rrepresents a C-Calkyl group claim 1 , and Rrepresents a —(CHOH—CHOH)—CHOH or —(CHOH—CHOH)—CHCHgroup in which q is an integer ranging from 1 to 10.6. The catalyst as claimed in claim 5 , wherein the coupling agent is γ-glycidoxypropyltrimethoxysilane.7. The catalyst as claimed in claim 1 , wherein the enzyme is selected from the group consisting of hydrolases claim 1 , lyases claim 1 , isomerases and oxidoreductases.8. The catalyst as claimed in claim 7 , wherein the enzyme is a hydrolase ...

MAGNETIC GLASS PARTICLES FOR USE IN BIOGAS PLANTS, FERMENTATION AND SEPARATION PROCESSES

A method for treating an organic and/or inorganic substrate utilizing a granular material made of a solid foam as support for an active component, for example a biocatalyst such as a microorganism or an enzyme. The solid foam has a continuous phase in which magnetizable particles are embedded, such that the support with the biologically active component immobilized thereon can be separated from a mixture with a magnetic separation device. 1. A method for treating an organic and/or inorganic substrate comprising:providing a substrate mixture which contains the organic and/or inorganic substrate in a reaction chamber;adding a magnetizable aggregate to the substrate mixture, wherein the magnetizable aggregate comprises a magnetizable support and an active component immobilized on the magnetizable support;converting the substrate mixture to a product mixture with the magnetizable aggregate; andseparating the magnetizable aggregate from the product mixture with a magnetic separation device,wherein the magnetizable support is present in the form of a particulate magnetizable support,wherein the particulate magnetizable support is constructed from a solid foam with a continuous phase which surrounds pores of the solid foam,wherein magnetizable areas are arranged in the continuous phase, andwherein the solid foam is closed-pore at least in the core of the magnetizable support.2. The method according to claim 1 , further comprising transferring the product mixture into a magnetic separation device in which the magnetizable aggregate is separated off from the product mixture.3. The method according to claim 1 , further comprising returning the magnetizable aggregate separated off from the product mixture to the reaction chamber.4. The method according to claim 1 , wherein the active component is a biocatalytically active system.5. The method according to claim 4 , wherein the biocatalytically active system is formed by at least one microorganism.6. The method according to ...

NOVEL USE OF LIPOLYTIC ENZYME FOR FORMATION OF ANTI-FINGERPRINT COATING, METHOD OF FORMING ANTI-FINGERPRINT COATING, SUBSTRATE COMPRISING THE ANTI-FINGERPRINT COATING FORMED BY THE METHOD, AND PRODUCT COMPRISING THE SUBSTRATE

Provided are a novel use of a lipolytic enzyme for forming anti-fingerprint coating, a method of forming anti-fingerprint coating including treating a substrate with a composition comprising the lipolytic enzyme, a substrate including the anti-fingerprint coating formed by the same method, and a product including the same. The anti-fingerprint coating can reduce contamination of display devices, appearances of electronic devices or building materials by fingerpris. 1. A method of forming anti-fingerprint coating , comprising treating a substrate with a composition comprising a lipolytic enzyme.2. The method according to claim 1 , wherein the lipolytic enzyme is a lipase.3. The method according to claim 2 , wherein the composition further includes at least one enzyme selected from the group consisting of a protease claim 2 , an amylase claim 2 , a cellulase claim 2 , and a lactase.4. The method according to claim 1 , wherein the substrate includes plastic or glass.5. The method according to claim 4 , wherein the plastic includes at least one polymer selected from the group consisting of polyester claim 4 , polypropylene claim 4 , polyethyleneterephthalate claim 4 , polyethylenenaphthalate claim 4 , polycarbonate claim 4 , triacetylcellulose claim 4 , olefin copolymers claim 4 , and polymethylmethacrylate.6. The method according to claim 1 , wherein the lipolytic enzyme is introduced to a surface of the substrate by adsorption claim 1 , covalent bonds claim 1 , or encapsulation.7. The method according to claim 6 , wherein the covalent bond is formed through a process including treating the surface of the substrate having at least one functional group selected from the group consisting of amino claim 6 , amide claim 6 , carboxyl claim 6 , aldehyde claim 6 , hydroxyl and thiol groups with a solution including a bifunctional cross-linker; and dipping the substrate in a buffer including the lipolytic enzyme.8. The method according to claim 6 , wherein the covalent bond is ...

Thiol-ene coupling chemistry for immobilization of biocatalysts

The present invention generally relates to immobilized biocatalysts or immobilized enzymes for use in carbon capture and sequestration technology. Thiol-ene chemistry is used to couple a biocatalyst, particularly carbonic anhydrase, to a substrate including a substrate, a solid support, a microparticle, a nanoparticle, or a combination thereof.

MICROORGANISM CONCENTRATION AGENT AND METHOD OF MAKING

A concentration agent for capture of microorganisms, including diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof, and methods for making the concentration agent. 1. A concentration agent comprising a surface treatment comprising titanium dioxide , fine-nanoscale gold or platinum , or a combination thereof on a particulate support selected from diatomaceous earth , metal oxide-modified diatomaceous earth , and combinations thereof.2. The concentration agent of claim 1 , wherein the surface treatment is titanium dioxide.3. The concentration agent of claim 1 , wherein the surface treatment is fine-nanoscale gold or platinum claim 1 , or a combination thereof.4. The concentration agent of claim 1 , wherein the metal oxide of the metal oxide-modified diatomaceous earth is selected from ferric oxide claim 1 , titanium dioxide claim 1 , zinc oxide claim 1 , aluminum oxide claim 1 , and combinations thereof; with the proviso that if the metal oxide is titanium dioxide claim 1 , then the surface treatment is fine-nanoscale gold or platinum claim 1 , or a combination thereof.5. An article comprising the concentration agent of having at least one microorganism strain bound to the concentration agent.6. A method of making a concentration agent according to claim 2 , the method comprising:(a) providing a particulate support selected from diatomaceous earth, metal oxide-modified diatomaceous earth, and combinations thereof;(b) providing a precursor compound comprising a hydrolysable titanium dioxide precursor;(c) combining the particulate support and the precursor compound; and(d) hydrolyzing the precursor compound such that titanium dioxide is deposited on the particulate support.7. The method of claim 6 , further comprising heating the particulate support having the titanium dioxide deposited thereon at a temperature in a range ...

METHOD FOR PROCESSING A SURFACE

A method for processing a surface involves depositing at least one class of enzymes () onto the surface (); introducing at least a reactant () into an environment of the surface (), and causing interaction between the enzymes () and the reactant (), thereby to cause processing of a region of the surface (), the processed region of the surface () being defined with respect to a region thereof that is proximate () to where the enzymes () have been deposited. 1. A method for processing a surface comprising the steps of:depositing at least one class of enzymes onto the surface;introducing at least a reactant into an environment of the surface, andcausing interaction between the enzymes and the reactant, thereby to cause processing of a region of the surface, the processed region of the surface being defined with respect to a region of the surface proximate to where the enzymes have been deposited.2. A method as claimed in wherein processing of the surface comprises one of: etching of the surface and deposition of at least a depositing species on the surface.3. A method as claimed in wherein claim 1 , in the step of causing interaction between the enzymes and the reactant claim 1 , the processed region of the surface is substantially outside the proximate region.4. A method as claimed in wherein claim 1 , in the step of causing interaction between the enzymes and the reactant claim 1 , the processed region of the surface is substantially in the proximate region.5. A method as claimed in wherein claim 1 , the spatial extension of the proximate region relative to where the enzymes have been deposited on the surface is adjustable by adjusting the concentration of the reactant claim 1 , the density of the enzymes claim 1 , the temperature of the environment of the surface or a combination thereof.6. A method as claimed in wherein claim 1 , in the step of depositing the enzymes claim 1 , at least another class of enzymes is additionally deposited on the surface.7. A method as ...

SILICA ENCAPSULATED BIOMATERIALS

The present invention relates to compositions for encapsulation of biomaterials in a silica-matrix. The present invention includes a composition for formation of a silica-matrix encapsulated biomaterial. The composition includes a reactive silicon compound and a biomaterial with a catalytic activity. When encapsulated in the silica-matrix, the biomaterial at least partially retains its catalytic activity. The present invention also relates to methods of making silica-matrix encapsulated biomaterials, and to methods of using silica-matrix encapsulated biomaterials, including methods of treating water or gas using the silica-matrix encapsulated biomaterials. 1. A composition for formation of a silica-matrix encapsulated biomaterial , comprising:a reactive silicon compound; and,a biomaterial with a catalytic ability;wherein the silica-encapsulated biomaterial at least partially retains its catalytic ability.2. The composition of claim 1 , wherein the catalytic ability of the silica-encapsulated biomaterial comprises conversion of atrazine into a different compound claim 1 , conversion of a fracking chemical to a less toxic compound claim 1 , or conversion of a gas to a less flammable claim 1 , less explosive claim 1 , or less toxic compound.3. The composition of claim 1 , wherein the biomaterial comprises at least one of a bacteria claim 1 , archaea claim 1 , protist claim 1 , fungi claim 1 , or enzyme.4. The composition of claim 1 , wherein enzyme causes at least part of the catalytic activity claim 1 , or wherein the biomaterial expresses at least one enzyme that causes the at least part of the catalytic activity.5. The composition of claim 4 , wherein the biomaterial expresses atrazine chlorohydrolase (AtzA).6. The composition of claim 1 , wherein the reactive silicon compound comprises a silanol.7. The composition of claim 1 , further comprising water.8. The composition of claim 1 , further comprising an organic precursor.9. The composition of claim 8 , wherein the ...

ORGANIC-INORGANIC NANOFLOWERS, THEIR SYNTHESIS AND USE

Organic-inorganic nanoflowers, methods of synthesis, and uses of the nanoflowers are described. It has been found that organic-inorganic nanoflowers can be grown in the presence of a solid substrate containing copper without the requirement for added copper ion. The method includes exposing bacteria to a solid substrate containing copper in the presence of an aqueous solution that contains phosphate ions. The aqueous solution can additionally contain chloride ions, similar to that of a phosphate-buffered saline composition. The solid substrate can be an alloy of copper and tin, and the substrate can have phosphorus incorporated into it. 1. A method for the production of nanoflowers , comprising:exposing bacteria to a solid substrate comprising copper in the presence of aqueous solution comprising phosphate ions.2. The method of claim 1 , wherein the aqueous solution further comprises chloride ions.3. The method of claim 2 , wherein the solid substrate comprises an alloy of copper and tin.4. The method of claim 3 , wherein the alloy comprises between about 5% and 40% tin by weight.5. The method of claim 4 , wherein the solid substrate further comprises phosphorus.6. The method of claim 5 , wherein the phosphorus content of the solid substrate is between 0.5% and 2% by weight of the substrate.7. The method of claim 6 , wherein the copper content of the solid substrate is between 60% and 95% by weight of the solid substrate.8. The method of claim 7 , wherein a surface of the substrate to which the bacteria is exposed has a surface roughness claim 7 , R claim 7 , of in a range of from about 1 μm to about 10 μm.9. The method of claim 8 , wherein said surface has a Rof no greater than about 30 μm.10. The method of claim 9 , wherein the bacteria comprises a gram negative bacterium.11E. coli.. The method of claim 10 , wherein the bacteria is12. The method of claim 11 , wherein the solution has a pH of between 7.0 and 7.4 claim 11 , the solution comprises chloride ion in a ...

SUBSTRATES COMPRISING SWITCHABLE FERROMAGNETIC NANOPARTICLES

In a process for producing organic substrate particles bonded to switchable ferromagnetic nanoparticles with a mean particle diameter in the range from 10 to 1000 nm, the ferromagnetic nanoparticles used are those nanoparticles which are nonferromagnetic at first, but become ferromagnetic when the temperature is lowered, these at first nonferromagnetic nanoparticles in dispersed form are bonded to the organic substance particles, and then the nanoparticles bonded to the substrate particles are made ferromagnetic as a result of the temperature being lowered. 1. (canceled)2. Diagnostic substrate particles , which comprise organic substrate particles bonded to switchable ferromagnetic nanoparticles with a mean particle diameter in the range from 10 to 1000 nm , said substrate particles having a specific bonding action for a substance to be analyzed.3. Diagnostic substrate particles according to claim 2 , which comprise switchable nanoparticles with a mean particle diameter in the range from 10 to 1000 nm claim 2 , wherein the switchable nanoparticles are in a nonferromagnetic state during a bonding of organic substrate to said switchable nanoparticles claim 2 , and the switchable nanoparticles are nanoparticles that have not been switched to the ferromagnetic state by a first cooling of the nanoparticles prior to said bonding.4. A medicament for hyperthermia treatment in the human or animal body claim 2 , comprising switchable ferromagnetic nanoparticles with a mean particle diameter in the range from 10 to 1000 nm claim 2 , which become ferromagnetic when the temperature is lowered.5. The medicament according to claim 4 , wherein the switchable ferromagnetic nanoparticle is magnetocaloric.6. The medicament according to for cancer treatment.7. Diagnostic substrate particles according to claim 2 , which are obtained by a process comprising bonding an organic substrate compound to switchable nanoparticles with a mean particle diameter in the range from 10 to 1000 nm ...

Composite Material

The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material. 1. A self-supporting composite material comprisinga substrate of discrete particles; anda network of interconnected mycelia cells extending through and around all of said discrete particles to fully colonize said substrate and bond said discrete particles together.2. A self-supporting composite material as set forth in wherein said particles are selected from the group consisting of at least one of vermiculite and perlite.3. A self-supporting composite material as set forth in wherein said particles are selected from the group consisting of at least one of straw claim 1 , hay claim 1 , hemp claim 1 , wool claim 1 , cotton and recycled sawdust.4. A self-supporting composite material as set forth in wherein said particles include synthetic insulating particles.5. A self-supporting composite material as set forth in wherein said synthetic insulating particles include foam based products and polymers.6. A self-supporting composite material as set forth in wherein said particles are in the form of fibers.7. A self-supporting composite material as set forth in further comprising elements in said substrate having a dimension 5 times larger than the mean diameter of the largest average particle size.8. A self-supporting composite material as set forth in wherein said elements are at least one of rods claim 7 , cubes claim 7 , panels claim 7 , and lattices.9. A self-supporting composite material ...

ISOLATION OF MICRONICHES FROM SOLID-PHASE AND SOLID SUSPENSION IN LIQUID PHASE MICROBIOMES USING LASER INDUCED FORWARD TRANSFER

A method for printing materials by: providing a receiving substrate; providing a target substrate having a photon-transparent support, a photon absorbent interlayer coated on the support, and a transfer material of a solid-phase microbiome sample coated on top of the interlayer opposite to the support; and directing photon energy through the transparent support so that the photon energy strikes the interlayer. A portion of the interlayer is energized by absorption of the photon energy, and the energized interlayer causes a transfer of a portion of the transfer material across a gap between the target substrate and the receiving substrate and onto the receiving substrate. 1. A method for printing materials comprising the steps of:providing a receiving substrate;providing a target substrate comprising a photon-transparent support, a photon absorbent interlayer coated on the support, and a transfer material comprising a solid-phase microbiome sample coated on top of the interlayer opposite to the support;providing a source of photon energy; and wherein a portion of the interlayer is energized by absorption of the photon energy; and', 'wherein the energized interlayer causes a transfer of a portion of the transfer material across a gap between the target substrate and the receiving substrate and onto the receiving substrate., 'directing the photon energy through the transparent support so that the photon energy strikes the interlayer;'}2. The method of claim 1 , further comprising:mixing the solid-phase microbiome sample with a liquid to form a suspension; andforming a layer of the transfer material by applying the suspension to the target substrate and optionally drying the suspension.3. The method of claim 1 , further comprising:forming a layer of the transfer material by applying a slice or portion of the microbiome sample to the target substrate and optionally adding a fluid between the microbiome sample and the target substrate.4. The method of claim 1 , wherein ...

HIERARCHICAL MAGNETIC NANOPARTICLE-ENZYME MESOPOROUS ASSEMBLIES EMBEDDED IN MACROPOROUS SCAFFOLDS

A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion. 122.-. (canceled)23. A method for epoxidation reactions of alkenes , the method comprising reacting alkenes in the presence of oxygen with a hierarchical catalyst composition comprising a continuous macroporous scaffold in which is incorporated self-assembled mesoporous aggregates of magnetic nanoparticles containing an oxygen-transfer enzyme embedded in mesopores of said mesoporous aggregates of magnetic nanoparticles , to produce an alkene oxide.24. The method of claim 23 , wherein said oxygen-transfer enzyme is a chloroperoxidase or a lipase.2539.-. (canceled)40. The method of claim 23 , further comprising magnetic particles claim 23 , not belonging to said mesoporous aggregates of magnetic nanoparticles claim 23 , embedded in said continuous macroporous scaffold.41. The method of claim 23 , wherein said continuous macroporous scaffold has a polymeric composition.42 ...

AMINOPEPTIDASE AND ITS USES

The present invention relates to the use of a TET protein as a N-terminus aromatic amino acid residues specific exopeptidase, said TET protein comprising the amino acid sequence as set forth in SEQ ID NO: 1. 1. A method for providing a N-terminus aromatic amino acid residues specific exopeptidase , wherein said a N-terminus aromatic amino acid residues specific exopeptidase is provided by a TET protein comprising , consisting essentially , or consisting of the amino acid sequence as set forth in SEQ ID NO: 1 ,or any homologous protein derived from said TET protein as set forth in SEQ ID NO: 1 by substitution, addition or deletion of at least one amino acid, provided that the derived protein retains at least 70% of identity with the amino acid sequence as set forth in SEQ ID NO: 1, and said derived protein retaining a N-terminus aromatic amino acid residues specific exopeptidase activity.2. A method for the modification of all or part of a polypeptide content of a substrate comprising peptides , polypeptides and/or proteins , wherein said modification is performed by at least a TET protein harboring at least a N-terminus aromatic amino acid residues specific exopeptidase activity , said at least a TET protein comprising , consisting essentially , or consisting of the amino acid sequence as set forth in SEQ ID NO: 1 ,or any homologous protein derived from said at least a TET protein as set forth in SEQ ID NO: 1 by substitution, addition or deletion of at least one amino acid, provided that the derived protein retains at least 70% of identity with the amino acid sequence as set forth in SEQ ID NO: 1, and said derived protein retaining a N-terminus aromatic amino acid residues specific exopeptidase activity.3. The method according to claim 1 , wherein said a TET protein or said derived protein originates from an extremophile microorganism belonging to the Methanococcales order.4Methanocaldococcus jannaschii.. The method according to claim 3 , wherein said extremophile ...

SYSTEMS AND METHODS FOR GROWING A BIOFILM OF PROBIOTIC BACTERIA ON SOLID PARTICLES FOR COLONIZATION OF BACTERIA IN THE GUT

The present invention provides a method, wherein the method forms a biofilm, wherein the biofilm comprises a population of at least one bacterial strain attached to particles, wherein the biofilm is configured to colonize a gut of a subject in need thereof for at least five days, when ingested by the subject, the method comprising: a. obtaining a population comprising at least one strain of bacteria; b. inoculating a growth medium containing particles with the population comprising at least one strain of bacteria; c. incubating the particles with the population comprising at least one bacterial strain for a time sufficient for the population of at least one strain of bacteria to attach to the particles; and d. culturing the population comprising at least one strain of bacteria attached to the particles in a growth medium, for a time sufficient to form a biofilm. 2. The method of claim 1 , wherein the population of at least one strain of bacteria attached to the particles is first cultured in the growth medium under static conditions claim 1 , followed by culture in the growth medium under flow conditions.3. The method of claim 1 , wherein the particles are porous particles ranging from 30 to 500 microns in diameter.4. The method of claim 1 , wherein the particles are selected from the group consisting of: seeds claim 1 , dicalcium phosphate claim 1 , and cellulose.5. The method of claim 1 , wherein said particle is a porous particle.6. The method of claim 1 , wherein the population comprising at least one bacterial strain is selected from gut microflora.7. The method of claim 1 , wherein said biofilm is acid tolerant.8. The method of claim 1 , wherein said biofilm is configured for pH dependent targeted release of the bacterial biofilm in the gastrointestinal tract.9. The method of claim 1 , wherein the biofilm is encapsulated with a compound configured to release the at least one bacterial strain at a pH found in the intestine of an animal.10. The method of claim 9 ...

Method for improving monoclonal antibody detection results

The present invention provides a detection method for a monoclonal antibody in a sample, comprising: (a) a step of capturing the monoclonal antibody in the sample and immobilizing the monoclonal antibody in pores of a porous body; (b) a step of bringing the porous body in which the monoclonal antibody is immobilized with nanoparticles on which protease is immobilized to conduct selective protease digestion of the monoclonal antibody; (c) a step of detecting, by a liquid chromatography mass spectrometry (LC-MS), peptide fragments obtained by the selective protease digestion; and (a′) a step of conducting a reduction reaction under an acidic condition after the step (a). By the present invention, further applicability of the detection method for the monoclonal antibodies using mass spectrometry is expected.

Systems and methods for growing a biofilm of probiotic bacteria on solid particles for colonization of bacteria in the gut

The present invention provides a method, wherein the method forms a biofilm, wherein the biofilm comprises a population of at least one bacterial strain attached to particles, wherein the biofilm is configured to colonize a gut of a subject in need thereof for at least five days, when ingested by the subject, the method comprising: a. obtaining a population comprising at least one strain of bacteria; b. inoculating a growth medium containing particles with the population comprising at least one strain of bacteria; c. incubating the particles with the population comprising at least one bacterial strain for a time sufficient for the population of at least one strain of bacteria to attach to the particles; and d. culturing the population comprising at least one strain of bacteria attached to the particles in a growth medium, for a time sufficient to form a biofilm.

METHOD FOR EX VIVO TREATING BLOOD OR PLASMA

A method for ex vivo treating blood or plasma is provided. The method includes (a) ex vivo contacting a blood or plasma with an enzyme composition to react the enzyme composition with the blood or plasma, wherein the enzyme composition is capable of eliminating electronegative low-density lipoprotein from the blood or plasma by the activity of the enzyme composition, and the enzyme composition is selected from a group consisting of: a first enzyme for eliminating a glycan residue of an electronegative low-density lipoprotein (LDL); a second enzyme for eliminating ceramide carried by a electronegative low-density lipoprotein (LDL); and a combination thereof; and (b) terminating contact between the blood or plasma and the enzyme composition to terminate the reaction of the enzyme composition with the blood or plasma. 1. A method for ex vivo treating blood or plasma , comprising: a first enzyme for eliminating a glycan residue of an electronegative low-density lipoprotein (LDL);', 'a second enzyme for eliminating ceramide carried by a electronegative low-density lipoprotein (LDL); and', 'a combination thereof; and, '(a) ex vivo contacting a blood or plasma with an enzyme composition to react the enzyme composition with the blood or plasma, wherein the enzyme composition is capable of eliminating electronegative low-density lipoprotein from the blood or plasma by the activity of the enzyme composition, and the enzyme composition is selected from a group consisting of(b) terminating contact between the blood or plasma and the enzyme composition to terminate the reaction of the enzyme composition with the blood or plasma.2. The method for ex vivo treating blood or plasma as claimed in claim 1 , wherein the step (a) is performed for about 0.25-8 hours.3. The method for ex vivo treating blood or plasma as claimed in claim 1 , wherein the step (a) is performed at about 4-40° C.4. The method for ex vivo treating blood or plasma as claimed in claim 1 , wherein the step (a) is ...

HUMAN FACTOR XIII AS A NORMALIZATION CONTROL FOR IMMUNOASSAYS

The present disclosure provides compositions and methods that are useful for normalizing the amount of signal detected in an assay, such as an immunoassay. The compositions and methods are useful for improving the accuracy of immunoassays, such as immunoassays that detect whether a subject is infected with a retrovirus such as HIV. 1. A kit for correcting for variations in sample processing and/or biological matrix effects when performing biological assays , said kit comprising a normalization factor immobilized on a solid support.2. The kit of claim 1 , wherein the normalization factor does not bind an analyte from a biological sample.3. The kit of claim 1 , wherein the normalization factor is hFXIII.4. The kit of claim 1 , further comprising an antibody to human Factor XIII immobilized on a solid support.5. The kit of claim 1 , further comprising tetramethylcadaverine rhodamine (TMRC) immobilized on a solid support.6. The kit of claim 1 , wherein the solid support is a bead or magnetic bead.7. The kit of claim 1 , further comprising a plurality of solid supports having binding members immobilize thereon that bind one or more analytes in a sample.8. The kit of claim 7 , wherein the plurality of solid supports are divided into subpopulations that are differentiable from each other by a differentiation parameter comprising a characteristic that is independent of the binding members immobilized on the solid supports claim 7 , the binding members immobilized on each subpopulation capable of binding to one analyte in the sample.9. A composition for correcting for variations in sample processing and/or biological matrix effects when performing biological assays claim 7 , said composition comprising a normalization factor coupled to a solid support.10. The composition of claim 9 , wherein the normalization factor does not bind an analyte from a biological sample.11. The composition of claim 9 , wherein the normalization factor is hFXIII.12. The composition of claim 9 , ...

AUTOMATED BIONANOCATALYST PRODUCTION

The present invention provides machines, compositions and methods for producing bionanocatalysts (BNCs) comprising one or more enzymes selected from a broad spectrum of industrially and medically important enzymes. The BNCs are self-assembled and magnetically immobilized enzymes. The machines, compositions, and methods are fully scalable from bench top to industrial manufacturing volumes. 2. The machine of claim 1 , wherein said MNP disruptor is a sonicator.3. The machine of claim 2 , wherein said sonicator further comprises a sonicator coil and a sonication container claim 2 , wherein said sonicator coil is operable to sonicate said MNPs within said sonication container.4. The machine of claim 2 , wherein the sonicator is an in-line sonicator.5. The machine of claim 2 , further comprising a cooling system operable for cooling said sonicator.6. The machine of claim 5 , wherein said cooling system is a water cooling system.7. The machine of claim 1 , wherein said MNP disruptor is operable to mechanically disrupt said MNPs.8. The machine of claim 1 , wherein said MNP disruptor is operable to magnetically disrupt said MNPs.9. The machine of claim 1 , wherein said MNP disruptor is operable to thermally disrupt said MNPs.10. The machine of claim 1 , wherein said BNC mixer comprises a mixing tee.11. The machine of claim 1 , wherein said BNC mixer comprises a mixing coil.12. The machine of claim 1 , wherein said enzyme pump sends said enzyme preparation to said BNC mixer via mechanical or gravitational force.13. The machine of claim 1 , wherein said MNP pump sends said MNP preparation to said MNP disruptor via mechanical or gravitational force.14. The machine of claim 1 , further comprising a magnetic scaffolding container operable for mixing a magnetic scaffolding preparation with said BNCs in said scaffolding container to produce BNCs in a level 2 assembly.15. The machine of claim 14 , wherein said magnetic scaffolding container is operable to mix said BNCS and said ...

Immobilized microbial agent for in situ restoration of contaminated sediments, preparation method and application thereof

An immobilized microbial agent for in situ restoration of contaminated sediments, composed of Hangjin clay 2 #-loaded conductive microorganisms, obtained by the following methods: 1) pretreating Hangjin clay 2 #to obtain particulate filler; 2) amplification culture of conductive microorganisms to a bacterial liquid to be inoculated, and adding the Hangjin clay 2 #pretreated in step 1 in a certain ratio, mixing under anaerobic conditions, removing the supernatant after standing, and obtaining the immobilized microbial agent; the conductive microorganisms are Geobacter sulfurreducens, Geobacter metallireducens and Shewanella . The invention also discloses a method for preparing the immobilized microbial agent and the application of in situ restoration of contaminated sediments.

METHOD FOR PREPARING SALIDROSIDE

The present invention provides a method for preparing salidroside. The present invention uses β-glucoside and CoFeOparticles to form a cross-linked aggregate capable of effectively catalyzing the reaction of β-D-ghucose and tyrosol, thereby increasing the yield of the salidroside. The steps of the preparation method of the present invention are simple and short, and the method is easy to operate and readily applicable to industrial production. 1. A method for preparing salidroside , comprising the following steps:{'sub': 2', '4, '(1) adding β-glucosidase into a phosphate-citric acid buffer solution, adding polyacrylamide cross-linked hollow CoFeOparticles, adding a settling agent, glutaraldehyde and sodium borohydride after oscillating, oscillating, centrifuging same at 320-480 rpm for 5-10 min, feeding same into a thermostatic water bath at 40-45° C., keeping the temperature and stirring same for 1-2 h, discharging, and collecting precipitates to obtain a β-glucosidase cross-linked aggregate;'}(2) adding β-D-glucose and tyrosol into a solvent, adding the buffer solution and the β-glucosidase cross-linked aggregate obtained in the step (1), and reacting to obtain a reaction solution; and(3) filtering the reaction solution obtained in the step (2), and carrying out reduced pressure distillation on a filtrate to obtain a crude product; and recrystallizing the crude product to obtain the salidroside.2. The method for preparing the salidroside according to claim 1 , wherein a method for preparing the polyacrylamide cross-linked hollow CoFeOparticles comprises the following steps:(1) adding 1-2 parts by weight of ammonium persulfate into deionized water which is 20-30 times of the weight of the ammonium persulfate, and uniformly stirring same; and{'sub': 2', '4, '(2) adding 40-45 parts by weight of methyl methacrylate into the deionized water which is 5-8 times of the weight of the methyl methacrylate, uniformly stirring same, feeding same into a reaction kettle, ...

Photocatalytic systems comprising graphene and associated methods

The present invention generally relates to photocatalytic systems comprising graphene and associated methods. Some embodiments are directed to systems comprising one or more layers of graphene having a first surface and a second, opposed surface. A light-absorbing complex may be associated with the first surface of the one or more graphene layers, and an electron donor complex may be associated with the light-absorbing complex. A catalytic complex may be associated with the first surface or the second surface of the one or more graphene layers. For example, the catalytic complex may catalyze the formation of hydrogen gas, NADH, and/or NADPH.

Matrix-mediated cell culture system

The invention relates to a matrix-mediated algal cell culture system comprising a porous matrix, a microalgal cell culture comprising cells immobilised on the porous matrix, and a vector including a nucleic acid sequence encoding a heterologous polypeptide of interest, wherein immobilisation of microalgal cells on the porous matrix results in the formation of interstitial spaces between the microalgal cells to allow for increased contact of the microalgal cells with the vector compared with a culture of microalgal cells which are not immobilised on a porous matrix, thereby allowing for more efficient transfection of the microalgal cells with the vector. The invention also relates to methods of screening single species of microalgae and mixed ecology samples for the ability to be transfected using the algal cell culture system, and to methods for the production of heterologous polypeptides using the matrix-mediated cell culture system.

METHOD OF PREPARING AN ORGANIC-INORGANIC HYBRID NANOFLOWER

The technical field of enzyme immobilization, and particularly, an organic-inorganic hybrid nanoflower and a preparation method thereof. The organic-inorganic hybrid nanoflower is a flower-like immobilized enzyme formed by self-assembly of a layered rare earth compound as an inorganic carrier and a biological enzyme as an organic component. The layered rare earth compound is Ln(OH)NO.nHO, where Ln is one or more of La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, or Y, and n=1.1-2.5. The biological enzyme is one or more of α-amylase, horseradish peroxidase, or laccase. A layered rare earth compound is used as the inorganic carrier for the organic biological enzyme to form the flower-like immobilized enzyme. The immobilized enzyme has better stability and higher catalytic performance when compared with a free enzyme. 1. (canceled)2. A method of preparing the organic-inorganic hybrid nanoflower of claim 1 , comprising:mixing a rare earth nitrate aqueous solution with a biological enzyme to obtain a mixed solution, wherein a rare earth ion in a rare earth nitrate is one or more of La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, or Y;adding ammonium nitrate and ammonia water in sequence to the mixed solution, and then aging to obtain an aged solution;centrifuging, washing, and drying the aged solution in sequence to obtain the organic-inorganic hybrid nanoflower.3. The method of claim 2 , wherein the rare earth ion in the rare earth nitrate aqueous solution has a molar concentration of 0.005-1 mol/L.4. The method of claim 2 , wherein the biological enzyme in the rare earth nitrate aqueous solution has a concentration of 0.001-3 mg/mL.5. The method of claim 2 , wherein the mixing is carried out at 15-60° C.6. The method of claim 2 , wherein the molar ratio of the ammonium nitrate to the rare earth ion is (1-10):1.7. The method of claim 2 , wherein the mixed solution after addition of ammonia water has a pH of 5-8.8. The method of claim 2 , wherein the aging is ...

PHOTOBIOREACTOR SYSTEMS AND METHODS

An algal growth system includes a first flexible sheet material mounted on a first frame in a first mounted geometry having a first height and a first width, the first height being greater than the first width, and a second flexible sheet material mounted on a second frame in a second mounted geometry having a second height and a second width, the second height being greater than the second width. The first flexible sheet and the second flexible sheet material are noncontiguous. The algal growth system also includes a motor, the motor being coupled with an actuator system, where the motor actuates the actuator system, and a reservoir. 1. A biomass growth system comprising:a. a frame;b. a first flexible sheet material mounted on a first frame in a first mounted geometry having a first height and a first width, wherein the first height is greater than the first width;c. a second flexible sheet material mounted on a second frame in a second mounted geometry having a second height and a second width, the first flexible sheet material and the second flexible sheet material being noncontiguous, wherein the second height is greater than the second width;d. a first drive shaft, the first drive shaft being coupled with the first frame, wherein the first drive shaft actuates the first flexible sheet material, and a second drive shaft, the second drive shaft being coupled with the second frame, wherein the second drive shaft actuates the second flexible sheet material;e. an actuator system, wherein the actuator system is coupled with the first drive shaft and the second drive shaft such that the first flexible sheet material and the second flexible sheet material are concurrently actuated;f. a motor, the motor being coupled with the actuator system, wherein the motor actuates the actuator system and the first drive shaft such that the first flexible sheet material is actuated and the second drive shaft such that the second flexible sheet material is actuated concurrently;g. a ...

Thermostable carbonic anhydrases and methods of use thereof

The present compositions and methods relate to a thermostable carbonic anhydrases, polynucleotides encoding the carbonic anhydrase, and methods of make and/or use thereof. Formulations containing the carbonic anhydrase are suitable for use in extracting carbon dioxide.

HEMATIN MODIFIED BILIRUBIN OXIDASE CATHODE

A cathode can include: an electrode substrate; a porphyrin precursor attached to the substrate; and an enzyme coupled to the electrode substrate to be associated with the porphyrin precursor, the enzyme reduces oxygen. The cathode can include a conductive material associated with the porphyrin precursor and/or the enzyme. The cathode can include 1-pyrenebutanoic acid, succinimidyl ester (PBSE) associated with the porphyrin precursor and/or the enzyme and/or the conductive material. The cathode can include 2,5-dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (DMY-Carb) associated with the 1-pyrenebutanoic acid, succinimidyl ester (PBSE) and/or the porphyrin precursor and/or the enzyme and/or the conductive material. The porphyrin precursor is attached to the substrate through covalent coupling. In some aspects, substrate is linked to the porphyrin precursor, the porphyrin precursor is linked to the conductive material, the conductive material is linked to the PBSE, the PBSE is linked to the DMY-carb, and the DMY-carb is linked to the enzyme. 1. A cathode comprising:an electrode substrate;a porphyrin precursor attached to the substrate; andan enzyme coupled to the electrode substrate so as to be associated with the porphyrin precursor, wherein the enzyme reduces oxygen.2. The cathode of claim 1 , comprising a conductive material associated with the porphyrin precursor and/or the enzyme.3. The cathode of claim 1 , comprising 1-pyrenebutanoic acid claim 1 , succinimidyl ester (PBSE) associated with the porphyrin precursor and/or the enzyme and/or the conductive material.4. The cathode of claim 3 , comprising 2 claim 3 ,5-dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (DMY-Carb) associated with the 1-pyrenebutanoic acid claim 3 , succinimidyl ester (PBSE) and/or the porphyrin precursor and/or the enzyme and/or the conductive material.5. The cathode of claim 4 , wherein the porphyrin precursor links a mixture of the enzyme claim 4 , conductive material claim 4 , PBSE claim 4 , ...

Symbiont for enhancement of plant performance

The present disclosure provides a novel endophyte, Serendipita vermifera ssp. bescii (“ S. bescii ”), uses thereof and methods incorporating the use thereof for enhancement of plant performance, particularly the use of S. bescii with phosphite as a phosphorous source. The present disclosure also provides methods for detecting the presence of and identifying S. bescii.

NOVEL METHOD AND DEVICE FOR WHOLE-CELL BACTERIAL BIO-CAPACITOR CHIP FOR DETECTING CELLULAR STRESS INDUCED BY TOXIC CHEMICALS

The present invention is directed to methods and a bio-capacitor sensing device for the detection of toxic chemicals using bacteria. The sensing platform comprises gold interdigitated capacitor with a defined geometry, a layer of carboxy-CNTs immobilized with viable cells as sensing elements. Also included are methods of making the bio-capacitor device and methods for detecting toxic chemicals that induce cellular stress response. The present innovation discloses the development of a bio capacitor chips immobilized with carboxy-CNTs tethered bacteria. In addition, the present invention also includes determination of behavior and characteristics of chemically stimulated bacteria on biochip using electric field including frequency and/or amplitude as controlling parameters. 1. A method of producing a bio-capacitor sensing device , the method comprising the steps of:a) providing a substrate;b) depositing a metal layer on the substrate to form a capacitor, wherein the metal layer comprises at least one electrode in interdigitated structure;c) patterning the metal layer on the capacitor;d) covalently attaching a layer of carboxylated carbon nanotubes (carboxy-CNTs) to the capacitor to form a carboxy-CNT activated capacitor;e) immobilizing viable cells to the carboxy-CNT activated capacitor,whereby the viable cells are sensing elements that are capable of adapting to respond with a target chemical,wherein the viable cells are monitored for stress imposed by the target chemical on the viable cells.2. The method of claim 1 , wherein the substrate is selected from the group consisting of silicon claim 1 , glass claim 1 , melted silica claim 1 , and plastics.3. The method of claim 1 , wherein in the electrode is a material selected from the group consisting of gold claim 1 , silver claim 1 , platinum claim 1 , palladium claim 1 , copper and indium tin oxide (ITO).4. The method of claim 1 , wherein the capacitor is a gold interdigitated capacitor.5Escherichia coli.. The method ...

Synthetic Matrix Assembled and Rapidly Templated Spheroids, Organoids and 3D Cell Cultures

Disclosed are spheroidal hybrid biodegradable materials containing low dimensional manganese dioxide (MnO) support structures and cells, methods of manufacture thereof, and methods of use thereof. 1. A biodegradable scaffolding material comprising a plurality of at least one of zero-dimensional , one-dimensional or two-dimensional manganese dioxide support structures , and a plurality of cells ,wherein the zero-dimensional, one-dimensional or two-dimensional manganese dioxide support structures each have a surface, and upon each surface is a coating comprising a plurality of cell adhesion molecules;wherein the zero-dimensional, one-dimensional or two-dimensional manganese dioxide support structures define a structure comprising a plurality of interstices, and the plurality of cells are disposed around and between the zero-dimensional, one-dimensional or two-dimensional manganese dioxide support structures and through the zero-dimensional, one-dimensional or two-dimensional manganese dioxide support structure interstices;wherein the cell adhesion molecules comprise a plurality of cell binding domains, have a binding affinity with the zero-dimensional, one-dimensional or two-dimensional manganese dioxide support structures, and promote the adhesion of the cells to the zero-dimensional, one-dimensional or two-dimensional manganese dioxide support structures; andwherein together, the support structures and the plurality of cells are configured to self-assemble to form at least one spheroid.2. The biodegradable scaffolding material of claim 1 , wherein the cell adhesion molecules comprise at least one of biopolymers or small molecules claim 1 ,wherein the small molecules have at least one of a mass of no more than 900 daltons, a molecular weight of no more than 1500, or a size of from about 0.5 nm to about 1.5 nm.3. The biodegradable scaffolding material of claim 1 , wherein the cell binding domains are peptides comprising at least one of an arginine-glycine-aspartic ...

APPARATUS FOR THE EXTRACORPOREAL TREATMENT OF BLOOD

An apparatus for the extracorporeal treatment of blood comprising an extracorporeal blood circuit (), a pump () configured to provide fluid displacement within the extracorporeal blood circuit, and a reaction chamber () connected to the extracorporeal blood circuit and configured to receive blood or plasma from the circuit and treat the blood or plasma. The reaction chamber comprises a protease enzyme immobilized to a support, in which the protease enzyme is specific for, and capable of irreversibly cleaving, a human C5a present in the blood or plasma, wherein the abundance of the human C5a in the treated blood or plasma is less than that in the untreated blood or plasma. The apparatus finds utility in the extracorporeal treatment of blood from patients with inflammatory conditions, especially auto-immune disease and sepsis. 1. A method for the extracorporeal treatment of blood , the method comprising removing blood or a blood fraction from a patient and reacting the blood or blood fraction with a protease enzyme that is specific for and capable of irreversibly cleaving functional C5a , thereby reducing an abundance of functional C5a in the blood or blood fraction , wherein the protease enzyme is immobilised to a support.23. The method of claim 1 , wherein the protease enzyme is a recombinant bacterial C5a protease comprising SEQ ID NO. claim 1 , or a functional variant thereof having at least 90% sequence identity with SEQ ID NO: 3.3. The method of claim 2 , wherein the functional variant of SEQ ID NO: 3 is SEQ ID NO: 4 or SEQ ID NO: 5.4. The method of claim 1 , wherein the reacting step is carried out with an apparatus comprising:an extracorporeal blood circuit;a pump configured to provide fluid displacement with the extracorporeal blood circuit; anda reaction chamber connected to the extracorporeal blood circuit, the reaction chamber configured to receive the blood or blood fraction from the extracorporeal blood circuit and to treat the blood or blood fraction, ...

TRANSGLUTAMINASE NANOFLOWERS

The present invention relates to novel immobilized nanoflowers made of Transglutaminase (TGase), a method for their synthesis and applications thereof. The Transglutaminase nanoflowers of the present invention are catalytically more active without mass transfer limitation, stable and reusable, wherein the overall flower is in the nano-range, is homo-dispersed and small-sized, and wherein Transglutaminase functions both as a cross-linking enzyme and as a seeding template. 1. Immobilized Transglutaminase nanoflowers comprising Transglutaminase and an inorganic metal , wherein the overall flower is in the nano-range , is homo-dispersed and small-sized , and whereinTransglutaminase functions both as a cross-linking enzyme and as a seeding template, and wherein said nanoflowers can tolerate cold temperature and possess higher durability in terms of organic solvents, temperature, pH, storage and operational stability, having higher catalytic efficiency of 6.92 fold as compared to free Transglutaminase without any mass transfer limitation.2. The nanoflower as claimed in wherein the size of the nanoflowers is <50 nm.3. The nanoflower as claimed in wherein the catalytic efficiency of the nanoflowers is 6.92 fold higher as compared to free TGase.4Streptomyces mobaraensis.. The nanoflower as claimed in wherein the source of Transglutaminase is extremophilic actinomycetes5. The nanoflower as claimed in claim 1 , wherein said inorganic metal is selected from the group comprising zinc claim 1 , iron claim 1 , manganese claim 1 , magnesium claim 1 , potassium claim 1 , calcium and copper.6. The nanoflower as claimed in claim 1 , wherein said inorganic metal is copper.7. The nanoflowers as claimed in claim 1 , wherein said nanoflower is homo-dispersed and less than 50 nm in size.8. The nanoflowers as claimed in claim 1 , wherein said nanoflowers have uniform cages/pockets in between the petals.9. The nanoflowers as claimed in claim 1 , wherein said nanoflower is bio-catalytically ...

Urease Purification And Purified Urease Products Thereof And Sorbent Cartridges, Systems And Methods Using The Same

Methods for purifying urease are described that make use of a precipitating agent such as ammonium sulfate to obtain urease as a precipitate. The method can involve use of a solubilizing agent, such as a citrate-containing solution, to dissolve the precipitate. The method can involve the use of a sugar to provide protected urease in a sugar-urease solution and immobilization of the urease. The method can involve freeze drying of the immobilized urease. A sugar-urease preparation and an immobilized urease, and a sorbent cartridge containing the urease are described as well as methods to conduct dialysis. 1. A method of purifying urease , comprising:a) mechanically separating an extract mixture to provide a separated solution, wherein the extract mixture comprises an extracting agent in solution and a comminuted source of urease;b) combining ammonium sulfate with the separated solution to precipitate urease in the separated solution to provide a precipitate-containing mixture;c) mechanically separating the precipitate-containing mixture to collect the precipitate;d) dissolving the precipitate collected to provide a urease-containing solution;e) combining the urease-containing solution with a sugar to provide a sugar-urease solution;f) combining the sugar-urease solution and a sorbent material to immobilize urease on the sorbent material to provide an immobilized urease preparation; andg) optionally freeze drying the immobilized urease preparation to provide an immobilized urease product.2. The method of claim 1 , wherein the source of urease is a botanical source of urease claim 1 , fungal source of urease claim 1 , algal source of urease claim 1 , bacterial source of urease claim 1 , invertebrate source of urease claim 1 , or any combination thereof.3. The method of claim 1 , wherein the source of urease comprises jack beans claim 1 , sword beans claim 1 , soy beans claim 1 , or any combination thereof.4. The method of claim 1 , wherein the source of urease is jack ...

Enzyme forming mesoporous assemblies embedded in macroporous scaffolds

A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion.

Method for immobilizing and releasing microorganism

Intended is to provide a more practical technique for immobilizing a microorganism using an adhesive protein AtaA derived from Acinetobacter sp. Tol 5. Provided is a method for attaching and releasing a microorganism, including (1) a step of contacting a microorganism, into which DNA encoding autotransporter adhesin derived from a microorganism belonging to the genus Acinetobacter has been introduced to impart or enhance non-specific adhesiveness, with a carrier under a high ionic strength and thus attaching the microorganism to the carrier; and (2) a step of releasing the microorganism from the carrier by washing under a low ionic strength.

MICROSOMAL BIOREACTOR FOR SYNTHESIS OF DRUG METABOLITES

Reusable microsomal biocatalytic systems (bioreactors) constructed on carbon nanostructure modified electrodes are provided. The bioreactors comprise stable, biologically active immobilized enzymes such as human cytochromes P 450 (CYPs) and their redox partner proteins, e.g. CYP-NADPH (reduced nicotinamide adenine dinucleotide phosphate) reductases (CPR), on the carbon nanostructure surface. The immobilized enzymes may be present in liver microsomes, such as human liver microsomes (HLM) or as bactosomes, S9 fractions, etc. The bioreactors are used, for example, for synthesizing metabolites of interest from compounds such as drugs that are catabolized by the enzymes. 1. A bioreactor device , comprisingan electrode coated with carbon nanostructured material, andone or more enzymes on the carbon nanostructured material.2. The bioreactor device of claim 1 , wherein the one or more enzymes are i) membrane-bound enzymes; or ii) enzymes that are not associated with a membrane.3. The bioreactor device of claim 2 , wherein the membrane-bound enzymes are present in a microsome claim 2 , a bactosome or an S9 fraction.4. The bioreactor device of claim 1 , wherein the enzymes are liver enzymes.5. The bioreactor device of claim 4 , wherein the liver enzymes are human liver enzymes.6. The bioreactor device of claim 1 , wherein the one or more enzymes are drug metabolizing enzymes.7. The bioreactor of claim 1 , wherein the enzymes comprise biocatalytically active cytochromes P 450 (CYPs) and/or CYP-NADPH (reduced nicotinamide adenine dinucleotide phosphate) reductases (CPRs).8. The bioreactor device of claim 1 , wherein the carbon nanostructured material is selected from the group consisting of single walled carbon nanotubes claim 1 , multiwalled carbon nanotubes claim 1 , Buckypaper and graphene nanostructures.9. The bioreactor device of claim 1 , wherein the electrode is a conductive metallic or non-metallic material.10. The bioreactor device of claim 1 , wherein the electrode is ...

Biofunctional materials

The present invention relates to compositions and processes in the field of self-cleaning system using digestive proteins. One composition includes a substrate, a digestive protein capable of decomposing a stain molecule, and a linker moiety bound to both said digestive protein and said substrate. The processes include binding a substrate to a surface and forming a linker moiety between a digestive protein and said substrate.

IMMOBILIZED PROTEASE WITH IMPROVED RESISTANCE TO CHANGE IN EXTERNAL ENVIRONMENT

The present invention provides a highly active protease that can be used in sample preparation for mass spectrometry of a protein and has excellent stability against a change in an external environment. 1. An immobilized protease obtained by immobilizing a crudely purified protease or a protease that has not been subjected to a self-digestion resistance treatment on surfaces of nanoparticles.2. The immobilized protease according to claim 1 , wherein the nanoparticle has a particle size of 100-500 nm.3. The immobilized protease according to any one of and claim 1 , wherein the nanoparticle is a magnetic nanoparticle.4. The immobilized protease according to any one of - claim 1 , wherein the protease is trypsin claim 1 , chymotrypsin claim 1 , lysyl endopeptidase claim 1 , V8 protease claim 1 , Asp N protease claim 1 , Arg C protease claim 1 , papain claim 1 , pepsin claim 1 , or dipeptidyl peptidase.5. The immobilized protease according to any one of - claim 1 , wherein the self-digestion resistance treatment is a reductive dimethylation treatment.6. The immobilized protease according to claim 5 , wherein the protease is trypsin or lysyl endopeptidase.7. A method for preparing an immobilized protease comprising a process of immobilizing a crudely purified protease or a protease that has not been subjected to a self-digestion resistance treatment on surfaces of nanoparticles.8. A method of imparting claim 5 , to a crudely purified protease or a protease that has not been subjected to a self-digestion resistance treatment claim 5 , resistance to a change in an external environment by immobilizing the protease on surfaces of nanoparticles.9. The method according to any one of and claim 5 , wherein the protease is trypsin claim 5 , chymotrypsin claim 5 , lysyl endopeptidase claim 5 , V8 protease claim 5 , Asp N protease claim 5 , Arg C protease claim 5 , papain claim 5 , pepsin claim 5 , or dipeptidyl peptidase.10. The method according to any one of and claim 5 , wherein ...

Method of Forming a Mycological Product

The method grows a mycelial mass over a three-dimensional lattice such that a dense network of oriented hyphae is formed on the lattice. Growth along the lattice results in mycelium composite with highly organized hyphae strands and allows the design and production of composites with greater strength in chosen directions due to the organized nature of the supporting mycelia structure. 1. A method of making a mycological product comprising the steps ofproviding a three-dimensional framework; andgrowing mycelium on said framework in an environment held at a temperature and humidity to stimulate mycelia growth for a time sufficient for the mycelia growth to form a dense network of oriented hyphae on said framework.2. A method as set forth in wherein said framework is digestible.3. A method as set forth in which further comprises the step of coating said framework with a mixture of starch and water prior to said step of growing mycelium on said framework.4ostreatus. A method as set forth in which further comprises the step of placing the coated framework on a bed of inoculum containing Plearotus on a nutrient carrier prior to said step of growing mycelium on said framework.5. A method of forming a product comprisingproviding a three-dimensional lattice having at least two grids oriented orthogonally to each other;coating the lattice with a mixture of starch and water;{'i': 'Plearotus ostreatus', 'thereafter placing the lattice in a bed of inoculum containing in a nutrient carrier;'}thereafter stimulating mycelium growth over and through said grids of the lattice to produce a dense network of hyphae; andallowing said hyphae to interweave over time to produce a mat of said thickly formed mycelia on said lattice.6. A method as set forth in further comprising the step of drying said mat. This is a Division of U.S. Ser. No. 13/856,086, filed Apr. 3, 2013 which is a Division of U.S. Ser. No. 12/001,556, filed Dec. 12, 2007, now U.S. Pat. No. 9,485,917.This invention claims ...

MICROSOMAL BIOREACTOR FOR SYNTHESIS OF DRUG METABOLITES

Reusable microsomal biocatalytic systems (bioreactors) constructed on carbon nanostructure modified electrodes are provided. The bioreactors comprise stable, biologically active immobilized enzymes such as human cytochromes P 450 (CYPs) and their redox partner proteins, e.g. CYP-NADPH (reduced nicotinamide adenine dinucleotide phosphate) reductases (CPR), on the carbon nanostructure surface. The immobilized enzymes may be present in liver microsomes, such as human liver microsomes (HLM) or as bactosomes, S9 fractions, etc. The bioreactors are used, for example, for synthesizing metabolites of interest from compounds such as drugs that are catabolized by the enzymes. 1. A bioreactor device , comprisingan electrode coated with carbon nanostructured material, andone or more enzymes on the carbon nanostructured material.2. The bioreactor device of claim 1 , wherein the one or more enzymes are i) membrane-bound enzymes; or ii) enzymes that are not associated with a membrane.3. The bioreactor device of claim 2 , wherein the membrane-bound enzymes are present in a microsome claim 2 , a bactosome or an S9 fraction.4. The bioreactor device of claim 1 , wherein the enzymes are liver enzymes.5. The bioreactor device of claim 4 , wherein the liver enzymes are human liver enzymes.6. The bioreactor device of claim 1 , wherein the one or more enzymes are drug metabolizing enzymes.7. The bioreactor of claim 1 , wherein the enzymes comprise biocatalytically active cytochromes P 450 (CYPs) and/or CYP-NADPH (reduced nicotinamide adenine dinucleotide phosphate) reductases (CPRs).8. The bioreactor device of claim 1 , wherein the carbon nanostructured material is selected from the group consisting of single walled carbon nanotubes claim 1 , multiwalled carbon nanotubes claim 1 , Buckypaper and graphene nanostructures.9. The bioreactor device of claim 1 , wherein the electrode is a conductive metallic or non-metallic material.10. The bioreactor device of claim 1 , wherein the electrode is ...

Magnetically immobilized metabolic enzymes and cofactor systems

The present invention provides compositions and methods for producing magnetic bionanocatalysts (BNCs) comprising metabolically self-sufficient systems of enzymes that include P450 monooxygenases or other metabolic enzymes and cofactor regeneration enzymes.

SELF-POWERED ENZYME MICROPUMPS

Drug delivery devices, sensors, and micropumps provided herein can utilize a reaction of an analyte triggered by an enzyme to drive fluid flow. In some cases, a drug delivery device can include a reservoir including a drug (e.g., insulin) and have an enzyme (e.g., glucose oxidase) positioned adjacent to said reservoir. The enzyme can catalyze a reaction of said analyte to drive a fluid flow adjacent to said reservoir to increase a release of the drug from said reservoir. A sensor for an analyte can include an enzyme bound to a surface and a flow meter to detect a flow of fluids adjacent to said surface. A self-powered enzyme micropump provided herein can provide precise control over flow rate in response to specific signals. 1. A drug delivery device adapted to release a drug in response to an analyte comprising:a. a reservoir comprising said drug; andb. an enzyme positioned adjacent to said reservoir, the enzyme being adapted to catalyze a reaction of said analyte to drive a fluid flow adjacent to said reservoir to increase a release of said drug from said reservoir.2. The drug delivery device of claim 1 , wherein said reservoir is a hydrogel.3. The drug delivery device of claim 2 , wherein said hydrogel is a positively charged hydrogel.4. The drug delivery device of claim 2 , wherein said hydrogel is a quaternary ammonium-terminated hydrogel.5. The drug delivery device of claim 2 , wherein said enzyme is bound to a surface of said hydrogel.6. The drug delivery device of claim 1 , wherein said drug is insulin and said analyte is glucose.7. The drug delivery device of claim 6 , wherein said enzyme is glucose oxidase.8. The drug delivery device of claim 1 , wherein the analyte is a nerve agent and the drug is an antidote for said nerve agent.9. The drug delivery device of claim 1 , wherein said enzyme catalyzes a decomposition reaction of said analyte.10. A sensor for an analyte comprising:a. an enzyme bound to a surface, the enzyme adapted to catalyze a reaction of ...

Fluorescent probe

A fluorescent probe is obtained via hydrolysis and condensation reaction using 3-glycidoxypropyl trimethoxysilane. The fluorescent probe includes a silicon oxide core and a self-assembled monolayer. The self-assembled monolayer has an epoxide group, and joins the silicone oxide core by a covalent bond. The epoxide group of the fluorescent probe can form a conjugated bond with a molecule with an amino group via an aminolysis reaction, forming a nanoparticle including the molecule and the fluorescent probe.

BIOSENSORS PRODUCED FROM ENZYMES WITH REDUCED SOLUBILITY AND METHODS OF PRODUCTION AND USE THEREOF

Multi-use biosensors are disclosed that include enzymes that have been modified to reduce the solubility thereof; the multi-use biosensors are used to detect analytes in fluidic biological samples, and the biosensors also maintain their enzyme activity after many uses. Multi-sensor arrays are disclosed that include multiple biosensors. Also disclosed are methods of producing and using these devices. 1. A multi-use biosensor for detecting the presence and/or concentration of at least one target analyte in a fluidic biological sample , the multi-use biosensor comprising:an electrode;a modified enzyme dispensed on at least a portion of the electrode, wherein the enzyme has been modified to reduce the solubility thereof through reaction of at least one functional group thereon with a reactant such that the modified enzyme is substantially insoluble in the fluidic biological sample and in calibration reagents utilized with the multi-use biosensor, and wherein the modified enzyme comprises an active site that interacts with the target analyte for detection of the target analyte; anda membrane disposed on at least a portion of the modified enzyme, wherein the membrane immobilizes the modified enzyme on the electrode.2. The multi-use biosensor of claim 1 , further defined as a potentiometric analyte biosensor.3. The multi-use biosensor of claim 1 , wherein the at least one functional group on the modified enzyme is selected from the group comprising an aldehyde- claim 1 , amine- claim 1 , carbonyl- claim 1 , carboxyl- claim 1 , hydroxyl- claim 1 , ketone- claim 1 , maleimide- claim 1 , sulfhydryl- claim 1 , and thiol-reactive group.4. The multi-use biosensor of claim 1 , wherein the reactant comprises a long chain biotin.5. The multi-use biosensor of claim 1 , wherein the membrane is permeable to the target analyte to be detected but substantially impermeable to the modified enzyme.6. The multi-use biosensor of claim 1 , wherein the membrane is formed of a material selected ...

IMMOBILIZATION OF PROTEINS WITH CONTROLLED ORIENTATION AND LOAD

Methods for immobilizing a protein or functional protein fragment on a surface in a controlled orientation, for immobilizing a protein or functional protein fragment on a surface with efficient immobilization loading of the protein or protein fragment, and for immobilizing a protein or functional protein fragment on a surface with retention of the activity of the protein or protein fragment. In the methods, a tetrazine-modified protein or a tetrazine-modified functional protein fragment is contacted with a trans-cyclooctene-modified surface to provide a surface having the protein or functional protein fragment immobilized thereon. Surfaces having a protein or functional protein fragment immobilized thereon obtainable by the method and methods for using the surfaces for measuring the binding of a ligand to a protein or functional protein fragment. 2. The method of claim 1 , wherein R is selected from substituted or unsubstituted C1-C6 alkyl group; R claim 1 , R claim 1 , R claim 1 , and Rare hydrogen; Ris hydrogen claim 1 , a counter ion claim 1 , or a carboxyl protecting group; and Ris hydrogen or an amine protecting group.3. The method of claim 1 , wherein the tetrazine-modified protein or functional protein fragment is prepared by genetic encoding using a non-canonical amino acid bearing a tetrazine moiety selected from 3-(6-methyl-s-tetrazin-3-yl)phenylalanine (Tet-v3.0-methyl) claim 1 , 3-(6-ethyl-s-tetrazin-3-yl)phenylalanine (Tet-v3.0-ethyl) claim 1 , 3-(6-isopropyl-s-tetrazin-3-yl)phenyl alanine (Tet-v3.0-isopropyl) claim 1 , 3-(6-t-butyl-s-tetrazin-3-yl)phenylalanine (Tet-v3.0-t-butyl) claim 1 , or 3-(6-n-butyl-s-tetrazin-3-yl)phenylalanine (Tet-v3.0-n-butyl).4. A method for efficiently immobilizing a protein or functional protein fragment on a surface claim 1 , comprising contacting a tetrazine-modified protein or a tetrazine-modified functional protein fragment with a trans-cyclooctene-modified surface to provide a surface having the protein or functional ...

METHOD FOR PRODUCING MICROBIOLOGIC AGENT, AND MICROBIOLOGIC AGENT

A method for producing a microbiologic agent is described. The method includes a step of adding biomass containing a target compound-degrading microorganism and an inorganic microparticle carrier to a medium containing a target compound. The microorganisms included in the biomass are carried on the inorganic microparticles, and the medium is cultured while monitoring an abundance ratio of the target compound-degrading microorganism in microorganisms carried on the inorganic microparticle carrier. After the abundance ratio of the target compound-degrading microorganism reaches a predetermined value, the inorganic microparticle carrier carrying the target compound-degrading microorganism is collected to obtain the microbiologic agent. 1. A method for producing a microbiologic agent carrying a target compound-degrading microorganism , comprising:a step of adding biomass including the target compound-degrading microorganism and an inorganic microparticle carrier to a medium including a target compound, allowing microorganisms included in the biomass to be carried on the inorganic microparticles, and culturing while monitoring an abundance ratio of the target compound-degrading microorganism in microorganisms carried on the inorganic microparticle carrier; anda step of, after the abundance ratio of the target compound-degrading microorganism reaches a predetermined value, collecting the inorganic microparticle carrier carrying the target compound-degrading microorganism to obtain the microbiologic agent;wherein the abundance ratio of the target compound-degrading microorganism is determined by a method comprising:a step of amplifying a predetermined region of a 16S rRNA gene of microorganisms carried on the inorganic microparticle carrier to obtain an amplified product; anda step of analyzing nucleotide sequence data of the amplified product, dividing the obtained nucleotide sequence data into a plurality of groups based on homologies among the nucleotide sequence data, ...

SILICA ENCAPSULATION OF UREOLYTIC BACTERIA FOR SELF-HEALING OF CEMENT-BASED COMPOSITES

One aspect of the present invention is directed to a method of preparing encapsulated ureolytic cells. This method includes blending freeze dried ureolytic cells and an aqueous solution to form a base mixture; mixing the base mixture with a silicate-forming compound to form a blend comprising silica encapsulated ureolytic cells; and freeze drying the silica encapsulated ureolytic cells. The present invention also relates to a method of producing a self-healing concrete. This method comprises providing silica encapsulated freeze-dried ureolytic cells; mixing the silica encapsulated freeze-dried ureolytic cells with cement to form a mixture; and blending the mixture with a calcium salt and a urea solution to form a concrete mixture. Also disclosed are silica encapsulated ureolytic cells, a method of making a concrete form, and a cured concrete product. 1. A method of preparing encapsulated ureolytic cells , said method comprising:blending freeze dried ureolytic cells and an aqueous solution to form a base mixture;mixing the base mixture with a silicate-forming compound to form a blend comprising silica encapsulated ureolytic cells; andfreeze drying the silica encapsulated ureolytic cells.2. The method of claim 1 , wherein the solution contains a base precursor.3. The method of claim 2 , wherein the base precursor is urea.4Sporosacina pateurii, Sporsacina ureae, Bacillus sphaericus, Bacillus pseudofirmus, Bacillus cohniiBacillus alkalinitrilicus.. The method of claim 1 , wherein the ureolytic cells are selected from the group consisting of claim 1 , and5. The method of claim 1 , wherein the silicate-forming compound is an organosilicate compound.6. The method of claim 5 , wherein the organosilicate compound is selected from the group consisting of tetraethyl orthosilicate claim 5 , tetramethyl orthosilicate claim 5 , tetraprophy orthosilicate claim 5 , and tetrabutyl orthosilicate.7. The method of further comprising:washing the mixture andrecovering the encapsulated ...

Bio-sensor and manufacturing method therefor

Disclosed is a manufacturing method for a bio-sensor. The present manufacturing method comprise the steps of: preparing an acidic solution comprising an enzyme and a molecule containing a catechol group; an immersing electrodes in the acidic solution and applying a voltage to the electrodes, thereby attaching a structure, which is generated by a reaction of the enzyme and the molecule containing a catechol group, to the electrodes.

RNA AFFINITY PURIFICATION

Provided herein, in some embodiments, are methods of purifying a nucleic acid preparation. The methods may comprise contacting a nucleic acid preparation comprising messenger ribonucleic acid with an RNase III enzyme that is immobilized on a solid support and binds to double-stranded RNA contaminants. 1. A method of purifying a nucleic acid preparation , comprisingcontacting a nucleic acid preparation comprising messenger ribonucleic acid (mRNA) with an RNase III enzyme that is immobilized on a solid support, under conditions that result in binding of the RNase III enzyme to double-stranded RNA.2. The method of claim 1 , wherein the mRNA is an in vitro-transcribed mRNA.3. The method of or claim 1 , wherein the preparation further comprises double-stranded RNA (dsRNA).4. The method of any one of - claim 1 , wherein the RNase III enzyme is catalytically inactive.5. The method of any one of - claim 1 , wherein the RNase III enzyme is a thermostable RNase III enzyme.6Thermotoga maritima. The method of claim 5 , wherein the thermostable RNase III enzyme is a RNase III enzyme.7. The method of any one of - claim 5 , wherein the RNase III enzyme comprises an amino acid sequence identified by SEQ ID NO: 3.8. The method of any one of - claim 5 , wherein the RNase III enzyme comprises an amino acid sequence having a modification at an amino acid position corresponding to E130 of the sequence identified by SEQ ID NO: 3.9. The method of claim 8 , wherein the RNase III enzyme comprises an amino acid sequence identified by SEQ ID NO: 4.10. The method of any one of - claim 8 , wherein the solid support comprises a carboxy-reactive resin or an amino-reactive resin.11. The method of any one of - claim 8 , wherein the mRNA comprises at least one chemical modification.12. The method of claim 11 , wherein the chemical modification is selected from pseudouridine claim 11 , N1-methylpseudouridine claim 11 , 2-thiouridine claim 11 , 4′-thiouridine claim 11 , 5-methylcytosine claim 11 , 5- ...

METHOD FOR TRANSFORMING ARSENIC SULFIDE SLAG AND CURING AND STABILIZING RESULTING COMPOUND BY MEANS OF MICROENCAPSULATION

The present disclosure provides a method for transforming an arsenic sulfide slag and curing and stabilizing the resulting compound by means of microencapsulation, comprising the following steps: (1) preparing arsenic trioxide from the arsenic sulfide slag as a raw material; (2) preparing 4-hydroxy-3-nitrophenylarsonic acid from the arsenic trioxide as a raw material; (3) preparing an iron-manganese dinuclear cluster metal arsenate compound having a porous structure; (4) subjecting the iron-manganese dinuclear cluster metal arsenate compound having a porous structure to surface coating with silicon; (5) synthesizing an Fe(0)/Al-SBA-15 mesoporous composite stabilizer by a hydrothermal reaction; and (6) subjecting the silicon coated iron-manganese dinuclear cluster metal arsenate compound to curing and stabilizing treatment by means of microencapsulation. The present disclosure involves transforming the arsenic sulfide slag into 4-hydroxy-3-nitrophenylarsonic acid and finally into a metal arsenate compound having a porous structure, which has the characteristics of good stability and low toxicity in comparison to conventional arsenic compounds. Thus, the toxicity associated with arsenic compounds can be greatly reduced. 1. A method for transforming an arsenic sulfide slag and curing and stabilizing the resulting compound by means of microencapsulation , comprising the following steps:(1) preparing arsenic trioxide from the arsenic sulfide slag as a raw material;(2) preparing 4-hydroxy-3-nitrophenylarsonic acid from the arsenic trioxide as a raw material;(3) induce-preparing an iron-manganese dinuclear cluster metal arsenate compound having a porous structure by using the 4-hydroxy-3-nitrophenylarsonic acid through transformation and solvent evaporation;(4) subjecting the iron-manganese dinuclear cluster metal arsenate compound having a porous structure to surface coating with silicon;(5) synthesizing an Fe(0)/Al-SBA-15 mesoporous composite stabilizer by a hydrothermal ...

Methods And Materials For Microorganism Capture

Material complexes that capture biologicals and methods of synthesizing and using such complexes composed of fluid-insoluble material and a receptor are provided herewith. The fluid-insoluble material has reactive functionality on its surface, including hydroxyl, amino, mercapto or eposy functionality material. The material can be agarose, sand, textile, or any combination thereof. The receptor is selected from the group consisting of mono-and poly-saccharides, heparin, or any combination thereof. Also provide are methods whereby releasing the captured biologicals and is controllable. 131-. (canceled)32. A method for separating a biological from a fluid sample comprising:mixing the fluid sample comprising the biological with a material complex comprising at least one hydroxyl-, amino-, mercapto or epoxy-containing material that is fluid-insoluble and at least one receptor selected from lactose, lactose derivative, mono- or poly-saccharide, heparin, chitosan, or any combination thereof, wherein the receptor is bound to the material via a linker;separating the biological from the fluid sample by immobilizing the biological to the material complex; andphysically separating the immobilized biological from the fluid sample by filtration, decantation, or a combination thereof while the biological remains immobilized to the material complex.33. The method of claim 32 , wherein the linker is a polymer comprising polyethylene glycol claim 32 , polyethylenimine claim 32 , polyvinylpyrrolidone claim 32 , acrylics claim 32 , acrylonitrile-butadiene-styrene claim 32 , polyacrylonitrile claim 32 , acetals claim 32 , polyphenylene oxides claim 32 , polyimides claim 32 , polystyrene claim 32 , polypropylene claim 32 , polyethylene claim 32 , polytetrafluoroethylene claim 32 , polyvinylidene fluoride claim 32 , polyvinyl chloride claim 32 , polyethylenimine claim 32 , polyesters claim 32 , polyethers claim 32 , polyamide claim 32 , polyorthoester claim 32 , polyanhydride claim 32 , ...

Method of Determining An Analyte Concentration in a Sample

A reagent composition for a biosensor sensor strip is disclosed that provides for rapid rehydration after drying. The composition includes porous particles and is preferably formed as a colloidal suspension. The dried reagent composition including porous particles may provide analytically useful output from the sensor strip in a shorter time period than observed from dried reagent compositions using solid particles. The output signal from the porous particle compositions may be correlated to the analyte concentration of a sample within about two seconds. In this manner, an accurate concentration determination of an analyte concentration in a sample may be obtained in less time than from sensor strips including conventional compositions. The reagent composition including the porous particles also may allow for the redox reaction between the reagents and the analyte to reach a maximum kinetic performance in a shorter time period than observed from conventional sensor strips. 134-. (canceled)35. A biosensor system for determining the concentration of an analyte in a sample , the biosensor system comprising:a sensor strip including a reservoir for receiving the sample, a working electrode, a counter electrode, a reagent composition comprising an enzyme, an electron transfer mediator and porous particles with a plurality of pores having an average diameter from 0.05 micrometer to 10 micrometer and a void volume of at least 20% (v/v); anda measurement device comprising a sensor interface, signal generator, and a processor, the signal generator providing an electrical input signal to the sensor interface, the processor being adapted to (i) direct the signal generator to provide the electrical input signal to the sensor interface, (ii) receive an output signal from the sensor interface, and (iii) measure the at least one output signal to obtain a current value from an excitation wherein the initial current is greater than those that follow in the decay and within less than ...

THERMOSTABLE CARBONIC ANHYDRASES AND METHODS OF USE THEREOF

The present compositions and methods relate to a thermostable carbonic anhydrases, polynucleotides encoding the carbonic anhydrase, and methods of make and/or use thereof. Formulations containing the carbonic anhydrase are suitable for use in extracting carbon dioxide. 1. A composition comprising a recombinant polypeptide that comprises an amino acid sequence having carbonic anhydrase activity , wherein said amino acid sequence has high thermostability and/or melting temperature under high ionic strength conditions , and wherein said amino acid sequence is at least 90% identical to the amino acid sequence of SEQ ID NO:28.29-. (canceled)10. The composition of claim 1 , wherein the amino acid sequence is at least 95% identical to the amino acid sequence of SEQ ID NO:28.11. The composition of claim 1 , wherein the polypeptide retains greater than 70% of the carbonic anhydrase activity when incubated for 5 or more hours at a pH of 8.5 to 9.5.12. The composition of claim 1 , wherein the polypeptide retains at least 80% of the carbonic anhydrase activity when incubated for about 24 hours at a temperature of about 25° C.13. The composition of claim 1 , wherein the polypeptide retains at least 90% of the carbonic anhydrase activity when incubated for about 24 hours at a temperature of about 40° C.14. The composition of claim 1 , wherein the polypeptide retains at least 40% of the carbonic anhydrase activity when incubated for about 3 hours at a temperature of about 50° C.15. The composition of claim 1 , wherein the polypeptide retains at least 25% of the carbonic anhydrase activity when incubated for about 3 hours at a temperature of about 40° C.16. The composition of claim 1 , wherein the polypeptide retains at least 50% of the carbonic anhydrase activity when incubated for 1 to 3 hours at a temperature of from 20° C. to 50° C. under high ionic strength conditions.1758-. (canceled)59. The composition of claim 1 , wherein the high ionic strength conditions comprise 1 M ...

Hierarchical magnetic nanoparticle-enzyme mesoporous assemblies embedded in macroporous scaffolds

A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion.

Protein films and methods of forming the same

Various embodiments disclosed relate to protein films and methods of making the same. In various embodiments, the present invention provides a method of making a protein film including placing on a substrate a protein solution, to form a precursor protein film. The protein solution includes one or more proteins. The method includes compressing the precursor protein film to form a protein film.

PROCESSES FOR PREPARING SILICA-CARBON ALLOTROPE COMPOSITE MATERIALS AND USING SAME

The present document describes a carbon allotrope-silica composite material comprising a silica microcapsule comprising a silica shell having a thickness of from about 50 nm to about 500 μm, and a plurality of pores, said shell forming a capsule having a diameter from about 0.2 μm to about 1500 and having a density of about 0.001 g/cm3 to about 1.0 g/cm3, wherein said shell comprises from about 0% to about 70% Q3 configuration, and from about 30% to about 100% Q4 configuration, or wherein said shell comprises from about 0% to about 60% T2 configuration and from about 40% to about 100% T3 configuration, or wherein said shell comprises a combination of T and Q configurations thereof, and wherein an exterior surface of said capsule is covered by a functional group; a carbon allotrope attached to said silica microcapsule. Also described is a carbon allotrope-silica composite material comprising a carbon allotrope attached to a silica moiety comprising a silica nanoparticle having a diameter from about 5 nm to about 1000 nm, wherein an exterior surface of said silica nanoparticle is covered by a functional group. 1. A carbon allotrope-silica composite material comprising:a silica microcapsule comprising:a silica shell having a thickness of from about 50 nm to about 500 μm, and a plurality of pores,{'sup': 3', '3, 'said shell forming a capsule having a diameter from about 0.2 μm to about 1500 μm, and having a density of about 0.001 g/cmto about 1.0 g/cm,'}wherein said shell comprises from about 0% to about 70% Q3 configuration, and from about 30% to about 100% Q4 configuration, orwherein said shell comprises from about 0% to about 60% T2 configuration and from about 40% to about 100% T3 configuration, orwherein said shell comprises a combination of T and Q configurations thereof, andwherein an exterior surface of said capsule is covered by a functional group;a carbon allotrope attached to said silica microcapsule.2. A carbon allotrope-silica composite material comprising: ...

MESOPOROUS CATALYSTS OF MAGNETIC NANOPARTICLES AND FREE-RADICALPRODUCING ENZYMES, AND METHODS OF USE

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described. 147.-. (canceled)48. A composition comprising lactoperoxidase immobilized within mesoporous aggregates of magnetic nanoparticles , wherein said mesoporous aggregates of magnetic nanoparticles have mesopores in which said lactoperoxidase is embedded , wherein said mesopores are located between magnetic nanoparticles in said aggregates of magnetic nanoparticles , and wherein said mesopores and aggregates of magnetic nanoparticles in said mesoporous aggregates are simultaneously produced by a self-assembly process in which said lactoperoxidase and magnetic nanoparticles are combined in solution.491. The composition of claim , wherein said mesoporous aggregates of magnetic nanoparticles have an iron oxide composition.501. The composition of claim , wherein said mesoporous aggregates of magnetic nanoparticles have a magnetic nanoparticle size distribution in which at least 90% of magnetic nanoparticles have a size of at least 3 nm and up to 30 nm , and an aggregated particle size distribution in which at least 90% of said mesoporous aggregates of magnetic nanoparticles have a size of at least 10 nm and up to 500 nm.511. The composition of claim , wherein said mesoporous aggregates of magnetic nanoparticles possess a saturated magnetization of at least 10 emu/g.521. The composition of claim , wherein said mesoporous aggregates of magnetic nanoparticles possess a remanent magnetization up to 5 emu/g. ...

COMPOSITE MATERIAL AND METHOD OF MANUFACTURING COMPOSITE MATERIAL

A composite material includes: an apatite crystal in the form of a tube; and a functional component accommodated in the apatite crystal tube and constituted by a material having physical properties different from those of the apatite crystal. The apatite crystal may be a monocrystal given by the general formula M(PO)X, where Mdenotes at least one element selected from the group consisting of divalent alkali earth metals and Eu, and X denotes at least one element or molecule selected from the group consisting of halogens and OH. 1. A composite material comprising:an apatite crystal in the form of a tube; anda functional component accommodated in the apatite crystal tube and constituted by a material of physical properties different from those of the apatite crystal.3. The composite material according to claim 1 , wherein the apatite crystal has a transmittance of at least 65% with respect to visible light.4. The composite material according to claim 1 , wherein the functional component is constituted by a material of higher rigidity than that of the apatite crystal.5. The composite material according to claim 1 , wherein the functional component is constituted by a photocatalytic substance.6. The composite material according to claim 1 , wherein the functional component is constituted by an enzyme.7. The composite material according to claim 1 , wherein the apatite crystal in outer form is a hexagonal prism claim 1 , and a hole-opening formed in either of top or bottom surfaces of the hexagonal prism is of hexagonal form.8. The composite material according to claim 1 , wherein the apatite crystal has a tube-hole inner diameter of 3 nm to 800 μm.9. The composite material according to claim 1 , wherein the apatite crystal is 1 μm to 1 mm in diameter.10. The composite material according to claim 1 , wherein the apatite crystal measures 2 μm to 4 mm lengthwise.11. A method of a manufacturing a composite material claim 1 , comprising:placing, tube-internally in an apatite ...

PHYSICAL DEPOSITION OF SILICEOUS PARTICLES ON PLASTIC SUPPORT TO ENHANCE SURFACE PROPERTIES

The present invention relates to products and method of preparing and using surface modified polymeric material having siliceous particles deposited thereon. The method and article are disclosed wherein a plastic substrate is provided with high surface area and increase of surface roughness. The methods for treating the surface are provided. 1. Surface modified polymeric material comprising a plurality of silica particles deposited and partially embedded on a surface thereof , wherein said silica particles are bioavailable for interaction with a microorganism or a biological molecule or complex , available for chemical interaction , available for chemical reaction , or a combination thereof.2. The surface modified polymeric material of claim 1 , wherein said plurality of silica particles is a plurality of one type of silica particle claim 1 , a plurality of at least one type of silica particle claim 1 , or a plurality of more than one type of silica particle.3. The surface modified polymeric material of claim 2 , wherein said polymeric material is a plastic material.4. The surface modified polymeric material of any one of - claim 2 , wherein said plurality of silica particles deposited and partially embedded on a surface thereof is deposited on said surface is at or over a melting point of said polymeric material.5. The surface modified polymeric material of any one of - claim 2 , wherein said silica particles are about 10% to about 90% partially embedded in the polymeric material.6. The surface modified polymeric material of any one of - claim 2 , wherein said silica particles cover from about 0.01% to 100% of said surface.7. The surface modified polymeric material of any one of - claim 2 , wherein said silica particle is a nanoparticle claim 2 , a microparticle claim 2 , a nanosphere claim 2 , a microsphere claim 2 , or combinations thereof.8. The surface modified polymeric material of any one of - claim 2 , wherein said silica particles have a diameter of from ...

ENZYME-IMMOBILIZED POROUS MEMBRANE AND PREPARATION METHOD OF ANTIBIOTICS USING THE SAME

The present disclosure relates to an enzyme-immobilized porous membrane and a preparation method of antibiotics using the same, and more specifically, to an enzyme-immobilized porous membrane prepared by immobilizing a specific enzyme through dead-end filtration, and a preparation method of antibiotics with a high yield using the enzyme-immobilized porous membrane. 1. An enzyme-immobilized porous membrane in which an enzyme promoting a synthesis reaction of an antibiotic substance is immobilized ,wherein the porous membrane is three-dimensionally interconnected by pores,the porous membrane forms a three-dimensional network by polymerizing a first monomer and a second monomer each having two to four functional groups,the functional group of the first monomer is an amino group,the functional group of the second monomer is an isocyanate group, an acyl halide group or an ester group,the first monomer and/or the second monomer has four functional groups, andthe enzyme is at least one selected from the group consisting of penicillin G acylase, penicillin V acylase, and cephalosporin C acylase.2. The enzyme-immobilized porous membrane according to claim 1 , wherein the antibiotic substance is a penicillin-based substance claim 1 , or a cephalosporin-based substance.3. A preparation method of antibiotics comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(B) permeating a derivative solution of an antibiotic substance through the enzyme-immobilized porous membrane according to .'}4. The preparation method according to claim 3 , wherein the derivative of the antibiotic substance includes a first derivative and a second derivative claim 3 ,the first derivative and the second derivative are mixed at a molar (M) ratio of 1:1 to 3,the first derivative and the second derivative are different from each other, and are each independently at least one selected from the group consisting of 6-aminopenicillanic acid, p-hydroxyphenylglycine methyl ester, 7- ...

METHOD OF IMMOBILIZING A CELL ON A SUPPORT USING COMPOUNDS COMPRISING A POLYETHYLENE GLYCOL MOIETY

The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support. 1. A method of immobilizing a cell on a support , the method comprising wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and', 'wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and', 'wherein the compound comprises a linking group;, 'a) providing a compound or salt thereof comprising one or more hydrophobic domains attached to a hydrophilic domain,'}b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; andc) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.2. The method of claim 1 , wherein the compound comprises one or more hydrophobic domains and a hydrophilic domain claim 1 ,wherein the one or more hydrophobic domains are ...

METAL-COATED SCAFFOLDS FOR TISSUE ENGINEERING

Metal nanoparticle-coated scaffolds are provided for use in tissue engineering. 1. A composition of matter comprising viable cells seeded on a scaffold , wherein an outer surface of said scaffold comprises a coating of metal nanoparticles and wherein more than 50% of said metal nanoparticles are positioned on said outer surface of said scaffold.2. A scaffold comprising fibers of decellularized extracellular matrix (ECM) , wherein an outer surface of said scaffold comprises a coating of metal nanoparticles , with the proviso that said metal nanoparticles are not crosslinked to said scaffold.3. A scaffold comprising decellularized omentum , wherein an outer surface of said scaffold comprises a coating of metal nanoparticles.4. The composition of matter or scaffold of claim 1 , wherein said metal nanoparticles comprise gold nanoparticles.5. The composition of matter of claim 1 , wherein said cells comprise electrically excitable cells.6. The composition of matter of claim 1 , wherein said scaffold comprises fibers.7. The composition of matter or scaffold of claim 1 , wherein said coating is between 2-20 nm in thickness.8. (canceled)9. The composition of matter of claim 6 , wherein said fibers comprise electrospun fibers.10. The composition of matter of claim 6 , wherein said fibers comprise a non-biodegradable polymer.11. The composition of matter of claim 1 , wherein said scaffold comprises decellularized extracellular matrix (ECM).12. The composition of matter of claim 6 , wherein said fibers comprise a biodegradable polymer.13. The composition of matter of claim 12 , wherein said biodegradable polymer is selected from the group consisting of polycaprolactone (PCL) claim 12 , polylactic acid (PLA) claim 12 , polyglycolic acid (PGA) claim 12 , and poly(Lactide-co-Glycolide) (PLGA).14. The composition of matter of claim 12 , further comprising an adhesive agent.15. The composition of matter of claim 14 , wherein said adhesive agent is selected from the group consisting ...

Reactive and sorbent materials

Reactive and sorbent materials including a non-encapsulated crosslinked biological material immobilized on a support matrix that includes a polyamine and at least one support material are described. The support material can be an inorganic or organic support material. The reactive and sorbent materials are formed by reacting the biological material with the polyamine, at least one support material, and a crosslinking agent. The materials exhibit enhanced properties generally, are capable of maintaining their reactive and sorbent properties in contact with digestive fluids, and exhibiting their reactive and sorbent properties as they pass throughout an organism's entire digestive system. Reactive and sorbent materials in contact with digestive juices at pH's ranging from about 3 to about 7 have maintained their reactive and sorbent properties for at least 4 hours.

Microbial Conductive Ceramics and Preparation Method and Application thereof

The disclosure discloses microbial conductive ceramics and a preparation method and application thereof, and belongs to the technical field of microorganisms and the technical field of semiconductor materials. The disclosure is based on ordinary insulating macroporous ceramics, using the means of cell immobilization and the principle of microbial adsorption, to prepare the microbial conductive ceramics including macroporous ceramics, microbes immobilized on the macroporous ceramics and metal ions adsorbed to the microbes. The microbial conductive ceramics have excellent performance, and the conductivity of the microbial conductive ceramics can reach 2.91×10S/m. At the same time, the cost of the microbial conductive ceramics is low, only 10% of the cost of conductive ceramics with the same conductivity. 1. A preparation method of microbial conductive ceramics , comprising: culturing microbes in a culture medium to a logarithmic growth phase or a stable phase to obtain a microbial bacterial solution; soaking macroporous ceramics in a hydrochloric acid or sodium hydroxide solution and then drying the macroporous ceramics for the first time to obtain pretreated macroporous ceramics; placing the pretreated macroporous ceramics into the microbial bacterial solution for shaking and then drying the macroporous ceramics for the second time to obtain macroporous ceramics with immobilized microbes; and passing a metal ion solution through the macroporous ceramics with immobilized microbes , and then drying the macroporous ceramics for the third time to obtain the microbial conductive ceramics , wherein the microbes comprise saccharomycetes , filamentous fungi or bacteria.2. The preparation method according to claim 1 , wherein when the microbes are saccharomycetes claim 1 , the culture time of the microbes in the culture medium is 12-60 h; when the microbes are filamentous fungi claim 1 , the culture time of the microbes in the culture medium is 24-72 h; and when the microbes ...

IMMOBILIZED PROTEINS AND USE THEREOF

The invention relates to an immobilized protein material comprising a protein that is immobilized on a glass material or organic polymer through affinity tag binding. The glass material may be a porous glass material such as (hybrid) controlled porosity glass. The invention also relates to the use of an immobilized enzyme material as a heterogeneous biocatalyst in chemical synthesis. The invention further relates to a method for the immobilization of affinity tagged proteins on a glass material or organic polymer, and to a method for the purification and isolation of affinity tagged proteins by the immobilization of such proteins on a glass material or organic polymer. 1. A method for catalyzing an enzyme-catalyzed cascade reaction , comprising(a) providing a solid support and two or more enzymes immobilized on said solid support,wherein the solid support has pores with diameter ranging between about 10 and 300 nm,wherein the solid support comprises non-swelling carrier material,wherein the non-swelling carrier material comprises a chelated metal ion, and wherein each of said immobilized enzymes comprises an affinity tag that binds to the chelated metal ion; and(b) bringing said immobilized enzymes into contact with a continuous flow of at least one substrate, thereby catalyzing the cascade reaction.2. The method according to claim 1 , wherein at least three enzymes are immobilized on said solid support.3. The method according to claim 1 , wherein the non-swelling carrier material is a porous organic polymer.4. The method according to claim 3 , wherein the porous organic polymer is chosen from the group consisting of polyethylene claim 3 , ultra-high molecular weight polyethylene (UHMWPE) claim 3 , high-density polyethylene (HDPE) claim 3 , polypropylene (PP) claim 3 , polytetrafluoroethylene (PTFE) claim 3 , polyvinylidene fluoride (PVDF) claim 3 , polystyrene claim 3 , polymethacrylate and poly(methyl methacrylate).5. The method according to claim 3 , wherein the ...

MICROCAPSULE OF SUSTAINABLE SELF-HEALING COAL MINE VENTILATION SEALING MATERIAL CRACK AND PREPARATION METHOD THEREOF

A microcapsule of sustainable self-healing coal mine ventilation sealing material crack. The microcapsule includes a microcapsule core material and a microcapsule wall material. The microcapsule core material is prepared using a bacterial lyophilized powder and a substrate. By using urease-producing bacteria, there is provided a method of protecting bacteria to survive a long time in the cement-based material, supplying sufficient nutrient substances and reducing the impact of the doping of bacteria on the mechanical property of the cement-based material. The bacterial lyophilized powder and substrate are prepared into microcapsules which are added into the cement-based material when the cement-based material is mixed. In this case, once concrete cracks, the microcapsules breaks and the spores in the material are activated to perform normal metabolism so as to induce precipitation of calcium carbonate continuously, thereby continuously realizing self-healing of coal mine ventilation sealing material cracks. 1. A microcapsule of sustainable self-healing coal mine ventilation sealing material crack , comprising a microcapsule core material and a microcapsule wall material , wherein the microcapsule core material is prepared using a bacterial lyophilized powder and a substrate.2. The microcapsule of sustainable self-healing coal mine ventilation sealing material crack according to claim 1 , wherein a mass ratio of the microcapsule core material to the microcapsule wall material is 1:1.2-1.5.3. The microcapsule of sustainable self-healing coal mine ventilation sealing material crack according to claim 2 , wherein a granule size of the microcapsule core material is 1.9-2.5 mm with a wall thickness being 60-100 μm.4bacillus sphaericus, sporosarcina pasteuriibacillus cereus.. The microcapsule of sustainable self-healing coal mine ventilation sealing material crack according to claim 3 , wherein the bacterial lyophilized powder in the microcapsule core material is selected ...

GLUCANASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM

The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature. 1220-. (canceled)221. An isolated , synthetic , or recombinant polypeptide comprising:(i) an amino acid sequence at least 70% or more or complete (100%) sequence identity to SEQ ID NO:352, or an enzymatically active fragment thereof, wherein the polypeptide or the enzymatically active fragment has a glucanase activity;(ii) an amino acid sequence encoded by a nucleic acid having at least 65% sequence identity to SEQ ID NO.: 351, wherein the polypeptide has glucanase activity;(iii) the amino acid sequence of (i), or (ii), lacking a signal sequence and/or a carbohydrate binding module(iv) the amino acid sequence of (i), (ii), or (iii), further comprising a heterologous sequence.222P. pastorisS. pombe.. The polypeptide of claim 221 , wherein the polypeptide comprises at least one glycosylation site; or claim 221 , wherein the polypeptide comprises at least one N-linked glycosylation site claim 221 , or claim 221 , wherein the polypeptide is glycosylated after being expressed in a or a223. A protein preparation comprising the polypeptide of claim 221 , wherein the protein preparation comprises a liquid claim 221 , a solid or ...

Method for Coating Surfaces by Enzymatic Reaction

The invention relates to a method for coating surfaces by enzymatic reaction of a biopolymer, wherein the method comprises the following steps: a) applying an enzyme to the surface of a substrate, and b) contacting the enzyme with the biopolymer to be deposited, wherein the enzyme cleaves the biopolymer, wherein the cleavage gives rise to at least two cleavage products of the biopolymer having different solubility in a solvent, and at least one cleavage product of the biopolymer having relatively low solubility is deposited on the surface of the substrate, and to a coated article obtainable by the method and to a coating composition comprising a biopolymer and at least one component selected from the group comprising binders, fillers, pigments and/or additives, and optionally a solvent. 1. A method for coating surfaces by enzymatic reaction of a biopolymer , the method comprising:a) applying an enzyme to the surface of a substrate, andb) contacting the enzyme with the biopolymer to be deposited, the enzyme cleaving the biopolymer, the cleavage giving rise to at least two cleavage products of the biopolymer having differing solubility in a solvent, and at least one cleavage product of the biopolymer having relatively low solubility being deposited on the surface of the substrate.2. The method as claimed in claim 1 , wherein the biopolymer is a protein claim 1 , protein complex or protein mixture and the enzyme is a protease.3. The method as claimed in claim 2 , wherein the protein is casein and the protease is selected from the group comprising chymosin and/or pepsin claim 2 , or the protein is fibrinogen and the protease is thrombin.4. The method as claimed in claim 1 , wherein the enzyme is applied to the surface by means of physical adsorption claim 1 , or ionic claim 1 , coordinate or covalent bonding claim 1 , it being possible to effect the covalent bonding to the surface via a polymeric spacer preferably selected from the group comprising polyethylene glycol ...

Soluble Intein Fusion Proteins And Methods For Purifying Biomolecules

The present invention relates to fusion proteins comprising an N-intein polypeptide and an N-intein solubilization partner, and affinity chromatography matrices comprising such fusion proteins, as well as methods of using same. 1. A split intein-based affinity chromatography system , comprising:a) a fusion protein comprising a C-intein polypeptide joined to a target molecule by a peptide bond; andb) an affinity chromatography matrix comprising an N-intein polypeptide capable of forming an active intein complex by associating with a fusion protein comprising the C-intein polypeptide and comprising at least one cysteine substituted with an amino acid selected from the group consisting of: threonine, lysine, asparagine, serine, methionine, and tyrosine, wherein the N-intein polypeptide is attached by covalent attachment to a solid support.2. The split intein-based affinity chromatography system of claim 1 , wherein the N-intein polypeptide is part of a fusion protein comprising the N-intein polypeptide and a N-intein solubilization partner joined by a peptide bond.3. The split intein-based affinity chromatography system of claim 1 , wherein the solid support is a chromatography resin.4. The split intein-based affinity chromatography system of claim 1 , wherein the chromatography resin includes a hydrophilic polyvinyl ether base.5. The split intein-based affinity chromatography system of claim 1 , wherein the solid support is a bead claim 1 , a hollow fiber claim 1 , a solid fiber claim 1 , a pad claim 1 , a gel claim 1 , a membrane claim 1 , a cassette claim 1 , a column claim 1 , a chip claim 1 , a slide claim 1 , a plate claim 1 , or a monolith.6. The split intein-based affinity chromatography system of claim 1 , wherein the solid support is a magnetic bead.7. The split intein-based affinity chromatography system of claim 1 , wherein the solid support comprises controlled pore glass claim 1 , silica claim 1 , zirconium oxide claim 1 , titanium oxide claim 1 , agarose ...

PHOTOBIOREACTOR SYSTEMS AND METHODS

An algal growth system can include a frame and a flexible sheet material that can have a substantially vertical orientation, where the flexible sheet material can be configured to facilitate the growth and attachment of algae, where the flexible sheet material can be supported by the frame. The algal growth system can include a first drive shaft, where the first drive shaft can be coupled with the frame, where the first drive shaft can support and actuate the flexible sheet material, a gear system, where the gear system can be coupled with the first drive shaft, a first roller, where the first roller can be coupled with the frame and can be configured to guide the flexible sheet material, and a drive motor, where the drive motor can be coupled with the gear system, where the drive motor can actuate the gear system and the at least one drive shaft such that the flexible sheet material can be actuated. 1. An algal growth method comprising the steps of: a. a frame;', 'b. a first flexible sheet material mounted on a first frame in a first mounted geometry, the first flexible sheet material having a substantially vertical orientation when mounted on the first frame such that the height of the first mounted geometry is greater than the width of the first mounted geometry;', 'c. a second flexible sheet material mounted on a second frame in a second mounted geometry, the first flexible sheet and the second flexible sheet material being noncontiguous, where the second flexible sheet material has a substantially vertical orientation when mounted on the second frame such that the height of the second mounted geometry is greater than the width of the second mounted geometry;', 'd. a first drive shaft, the first drive shaft being coupled with the first frame, wherein the first drive shaft supports and actuates the first flexible sheet material, and a second drive shaft, the second drive shaft being coupled with the second frame, wherein the second drive shaft supports and ...

Microbial Fermentation Methods and Compositions

The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.

METHODS FOR SELECTING PHOSPHATASE SELECTIVE AND NON-SELECTIVE PHOSPHATASE INHIBITORS

The present invention discloses a method to discover selective inhibitors of phosphatases. Thus the invention provides a method for screening a test compound to determine whether the compound binds a holophosphatase selectively or non-selectively comprising: i) providing a first holophosphatase wherein said holophosphatase is captured/immobilised; ii) testing a test compound for its ability to bind to the first holophosphatase; iii) providing a second holophosphatase wherein said second holophosphatase is captured/immobilised; iv) testing the same test compound for its ability to bind to the second holophosphatase; v) comparing the binding of the test compound to said first holophosphatase with the binding to said second phosphatase wherein a compound that binds a holophosphatase selectively will bind to said first holophosphatase but not said second holophosphatase; or will bind to said second holophosphatase but not said first; or wherein a compound that binds a holophosphatase non-selectively will bind to both said first holophosphatase and said second holophosphatase. 1. A method for screening a test compound to determine whether the compound binds a holophosphatase selectively or non-selectively comprising:i) providing a first holophosphatase wherein said holophosphatase is captured/immobilised;ii) testing a test compound for its ability to bind to the first holophosphatase;iii) providing a second holophosphatase wherein said second holophosphatase is captured/immobilised;iv) testing the same test compound for its ability to bind to the second holophosphatase;v) comparing the binding of the test compound to said first holophosphatase with the binding to said second phosphatasewherein a compound that binds a holophosphatase selectively will bind to said first holophosphatase but not said second holophosphatase; or will bind to said second holophosphatase but not said first; or wherein a compound that binds a holophosphatase non-selectively will bind to both said ...

APPARATUS FOR THE EXTRACORPOREAL TREATMENT OF BLOOD

An apparatus for the extracorporeal treatment of blood comprising an extracorporeal blood circuit (), a pump () configured to provide fluid displacement within the extracorporeal blood circuit, and a reaction chamber () connected to the extracorporeal blood circuit and configured to receive blood or plasma from the circuit and treat the blood or plasma. The reaction chamber comprises a protease enzyme immobilized to a support, in which the protease enzyme is specific for, and capable of irreversibly cleaving, a human C5a present in the blood or plasma, wherein the abundance of the human C5a in the treated blood or plasma is less than that in the untreated blood or plasma. The apparatus finds utility in the extracorporeal treatment of blood from patients with inflammatory conditions, especially auto-immune disease and sepsis. 1. A method for the extracorporeal treatment of blood , the method comprising removing blood or a blood fraction from a patient and reacting the blood or blood fraction with a protease enzyme that is specific for and capable of irreversibly cleaving functional C5a , thereby reducing an abundance of functional C5a in the blood or blood fraction , wherein the protease enzyme is immobilised to a support.2. The method of claim 1 , wherein the protease enzyme is a recombinant bacterial C5a protease comprising SEQ ID NO. 3 claim 1 , or a functional variant thereof having at least 90% sequence identity with SEQ ID NO: 3.3. The method of claim 2 , wherein the functional variant of SEQ ID NO: 3 is SEQ ID NO: 4 or SEQ ID NO: 5.5. The method of claim 4 , wherein the protease enzyme is a recombinant bacterial C5a protease comprising SEQ ID NO. 3 claim 4 , or a functional variant thereof having at least 90% sequence identity with SEQ ID NO: 3.6. The method of claim 5 , wherein the functional variant of SEQ ID NO: 3 is SEQ ID NO: 4 or SEQ ID NO: 5.7. The method of claim 4 , wherein the apparatus further comprises separating means adapted to separate the blood ...

MAGNETIC NANOPARTICLE MICROBIAL COMPOSITE WITH CORE-SHELL STRUCTURE, PREPARATION METHOD THEREOF, AND ITS APPLICATION IN THE TREATMENT OF AZO DYES

The invention discloses a magnetic nanoparticle microbial composite material with a core-shell structure and a preparation method thereof as well as application of the magnetic nanoparticle microbial composite material in azo dye treatment. The preparation method comprises the following steps: putting ferroferric oxide into an ethanol solution with ferric trichloride and trimesic acid; carrying out layer-by-layer self-assembly and ultrasonic condition reaction in sequence to prepare modified ferroferric oxide nanoparticles; then loading the modified composite material on the surfaces of microorganisms. The composite material prepared by the preparation method disclosed by the invention has the advantages of high adsorption effects and capability of carrying out local enrichment on the dye; meanwhile, magnetic separation can be performed, and thereby the azo dye can be removed efficiently. 1. A preparation method of magnetic nanoparticle microbial composite material with core-shell structure , characterized in comprising the following steps:(1) dissolving a mixture of ferric chloride, sodium citrate, sodium acetate, and ethylene glycol at 180° C. to 200° C. for 8 to 10 hours to prepare ferroferric oxide nanoparticles; then dispersing the ferroferric oxide nanoparticles to the alcohol solution, and then adding thioglycolic acid ultrasonically reacting to prepare modified ferroferric oxide nanoparticles;(2) the modified ferroferric oxide nanoparticles are sequentially reacted with ferric chloride and trimesic acid to prepare core-shell structured magnetic nanoparticles; said modified ferroferric oxide nanoparticles are sequentially reacted with ferric chloride and trimesic acid for 8 to 20 times;(3) the core-shell structured magnetic nanoparticles are modified to the surface of microbial to prepare magnetic nanoparticle microbial composite with core-shell structure.2. The preparation method of magnetic nanoparticle microbial composite material with core-shell structure ...

THREE-DIMENSIONAL POROUS STRUCTURE MADE OF NANOFIBRE WEB FRAGMENTS AND METHODS FOR PRODUCTION THEREOF

A three-dimensional, porous structure made of fragments of a nanofibre web is provided. Furthermore, a method for the production of a three-dimensional, porous structure made of nanofibre web fragments is proposed. The three-dimensional, porous structure is used for example in medicine, preferably in regenerative medicine. Furthermore, the structure according to the invention made of fragments of a nanofibre web can be used for the treatment of tissue damage, for the immobilisation of biological cells, for the construction of biological tissue and as a biological filler in vitro and also in vivo. 1. A method for the production of a three-dimensional porous structure made of fragments which consist of a web made of nanofibres , comprisinga) cutting a dry or wet web made of nanofibres into fragments with a laser and suspending the web fragments in a liquid medium; orb) cutting a web made of nanofibres which is present in a liquid medium into fragments with a laser, as a result of which a suspension of web fragments in the liquid medium is produced; andc) at least partial removal of the liquid medium, a three-dimensional, porous structure being formed from web fragments by means of self-organisation.2. The method according to claim 1 , wherein in step c) claim 1 , a gel claim 1 , a paste or a solid structure claim 1 , in particular a structure serving as biological extracellular matrix is formed.3. The method according to wherein step a) or step b) claim 1 , the web is cut into polygonal web fragments claim 1 , web fragments with rounded edges claim 1 , round web fragments claim 1 , triangular web fragments claim 1 , square web fragments claim 1 , rectangular web fragments claim 1 , rhomboid web fragments and/or trapezoidal web fragments.4. The method according to claim 1 , wherein step a) or step b) claim 1 , fragments with an edge length of 50 μm to 100 mm and/or with a surface area ≦1 mmare produced.5. The method according to claim 1 , wherein after step a) or step ...

Photobioreactor systems and methods

An algal growth system includes a first flexible sheet material mounted on a first frame in a first mounted geometry, the first flexible sheet material having a substantially vertical orientation when mounted on the first frame such that a first height of the first mounted geometry is greater than a first width of the first mounted geometry. The algal growth system also includes a first drive shaft coupled with the first frame, an actuator system coupled with the first drive shaft, a motor coupled with the actuator system. The motor actuates the actuator system and the first drive shaft such that the first flexible sheet material is actuated. The algal growth system also includes a liquid source consisting of a dripper or a mister. The liquid source is configured to direct a contacting liquid to the first flexible sheet material.

IMMOBILIZED CYCLOALIPHATIC PEPTIDE ACYLTRANSFERASE AND PREPARATION METHOD AND USES THEREOF

Disclosed in the present invention are an immobilized cycloaliphatic peptide acyltransferase and a preparation method and use thereof. The cycloaliphatic peptide acyltransferase is immobilized on a carrier; the cycloaliphatic peptide acyltransferase is derived from natural or artificial mutants or variants thereof, or can be obtained by introducing a foreign cyclic acyltransferase gene and transforming thereafter; the material of the carrier is selected from an inorganic carrier or a polypropylene resin carrier. Also disclosed in the present invention are the preparation method for the immobilized cycloaliphatic peptide acyltransferase and uses thereof. 1. An immobilized cycloaliphatic peptide acyltransferase.2. The immobilized cycloaliphatic peptide acyltransferase according to claim 1 , wherein cycloaliphatic peptide acyltransferase is immobilized on a carrier; and the carrier is selected from inorganic carrier claim 1 , or porous claim 1 , hydrophilic enzyme carrier.3. The immobilized cycloaliphatic peptide acyltransferase according to claim 2 , wherein claim 2 , based on the total weight of the inorganic carrier claim 2 , the content of SiOis more than 50 wt % claim 2 , and the content of AlOis more than 1 wt %.4. The immobilized cycloaliphatic peptide acyltransferase according to claim 2 , wherein the porous hydrophilic enzyme carrier is selected from the following: a enzyme-carrier in which polymethacrylate is used as matrix with bonded epoxide or containing amino functional groups.7. A method for preparing the immobilized cycloaliphatic peptide acyltransferase according to claim 1 , comprising the following steps:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'mixing a solution containing free cycloaliphatic peptide acyltransferase with a carrier to obtain the immobilized cycloaliphatic peptide acyltransferase according to .'}8. The method according to claim 7 , wherein said cycloaliphatic peptide acyltransferase is derived from natural or artificial ...

CELL-COLLAGEN-SILICA COMPOSITES AND METHODS OF MAKING AND USING THE SAME

Soluble, self-assembling collagens derived from tissues are extensively characterized such that one can predict and customize the final collagen-fibril matrix with respect to fibril microstructure (i.e., fibril density, interfibril branching), viscoelasticity and proteolytic degradability. As shown herein these matrices template and direct the deposition of mesoporous silica at the level of individual collagen fibrils. The fibril density, silicic acid concentration, and time of exposure to silicifying solution were varied and the resulting hybrid materials were analyzed by scanning electron microscopy, energy-dispersive x-ray spectroscopy, and rheology. Microstructural properties of the collagen-fibril template are preserved in the silica surface of hybrid materials. Results for three different collagen fibril densities, corresponding to shear storage moduli of 200 Pa, 1000 Pa, and 1600 Pa, indicate that increased fibril density increases the absolute amount of templated silica when all other silica synthesis conditions are kept constant. The mechanical properties of the hybrid material are dominated by the presence of the silica coating rather than the starting collagen matrix stiffness. 1. A composition comprising:at least one biological material;a tuned collagen material comprised of individual fibril branches, and having an inner portion, and an outer portion, wherein the inner portion is configured to at least partially encapsulate the at least one biological material; anda silica layer, wherein the silica layer is coupled to the outer portion of the collagen material.2. The composition of claim 1 , wherein the tuned collagen material is self-assembled or polymerized from oligomers.3. The composition of claim 1 , wherein the at least one biological material includes at least one biological material selected from the group consisting of: bioelements claim 1 , biomolecules claim 1 , biogenic substances claim 1 , biotic materials claim 1 , natural materials claim ...

Biodegradable polyester composition and uses thereof

The present invention relates to plastic composition comprising at least one polyester, biological entities having a polyester-degrading activity and at least an anti-acid filler, wherein the biological entities represent less than 11% by weight, based on the total weight of the plastic composition, and uses thereof for manufacturing biodegradable plastic articles.

Microbial Fermentation Methods and Compositions

The present invention provides methods for the cultivation of the genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture. 123-. (canceled)24MethylobacteriumMethylobacteriumMethylobacteriumMethylobacteriumMethylobacterium. A method for treating a plant or a plant part with comprising the step of applying to said plant or plant part a composition comprising a fermentation product comprising a solid substance wherein a mono-culture or co-culture of is adhered thereto by having been grown on said solid substance , wherein said solid substance comprises a plurality of particles of about 1 micron to about 1000 microns in average length or average diameter and is not a photosynthetic microorganism , and wherein said fermentation product is essentially free of contaminating microorganisms other than the , and wherein said composition (i) has a titer of at least 5×10colony-forming units per ml of said composition to about 6×10colony-forming units per ml of said composition for a fermentation broth , or (ii) has a titer of at least 5×10colony-forming units per gram of said composition to about 5×10colony-forming units per gram of said composition for a dry composition.25. The method of claim 24 , wherein said composition further comprises at least one of an agriculturally acceptable adjuvant or an agriculturally acceptable excipient.26. The method of claim 24 , wherein said composition is an essentially dry product claim 24 , an emulsion claim 24 , or a suspension.27Methylobacterium. The method of claim 24 , wherein said plant part is a seed and said composition has a titer of at least about 5×10colony-forming units per gram of said composition to about 5×10colony-forming units per gram of said composition.28. The method of claim 24 , wherein said plant part is a seed claim 24 , stem ...

IMMOBILIZED POLY(N)POLYMERASE

The present invention relates to an immobilized poly(N)polymerase (PNP), methods of producing said PNP and uses thereof. Further disclosed is an enzyme reactor and kit comprising the PNP for producing polynucleotidylated ribonucleic acid poly(N)RNA)molecules which are useful in gene therapy, immunotherapy, protein replacement therapy and/or vaccination. 1. Poly(N)polymerase characterized in that the poly(N)polymerase is a bacterial poly(N)polymerase and immobilized onto a solid support.2. The poly(N)polymerase according to claim 1 , wherein the poly(N)polymerase is selected from the group consisting of poly(A)polymerase claim 1 , poly(U)polymerase claim 1 , poly(G)polymerase and poly(C)polymerase.3. The poly(N)polymerase according to or claim 1 , wherein the poly(N)polymerase is a poly(A)polymerase.4. The poly(N)polymerase according to any one of the preceding claims claim 1 , wherein the poly(N)polymerase is immobilized onto the solid support by covalent binding claim 1 , affinity binding claim 1 , or physical adsorption.5. The poly(N)polymerase according to any one of the preceding claims claim 1 , wherein the poly(N)polymerase is immobilized onto the solid support by covalent binding.6. The poly(N)polymerase according to any one of the preceding claims claim 1 , wherein claim 1 , the poly(N)polymerase is immobilized by covalent binding to a thiol-activated solid support claim 1 , haloacetyl functionalized solid support claim 1 , epoxy-functionalized solid support claim 1 , pyridyl disulfide-functionalized solid support claim 1 , maleimide-activated solid support or a mixture thereof claim 1 , preferably the poly(N)polymerase is immobilized by covalent binding to a thiol-activated solid support claim 1 , haloacetyl functionalized solid support claim 1 , pyridyl disulfide-functionalized solid support claim 1 , maleimide-activated solid support or a mixture thereof.7. The poly(N)polymerase according to any one of the preceding claims claim 1 , wherein the poly(N) ...

MAGNETICALLY IMMOBILIZED MICROBIOCIDAL ENZYMES

The present invention provides compositions and methods for reducing microbial contamination or infection in plants, animals, fabrics, and products therefrom. The present invention also provides compositions and methods for reducing human infections. In particular, it provides solid magnetic nanoparticles comprising bacteriostatic, bactericidal, fungistatic, or fungicidal enzymes in one component, and substrates for the enzymes in another component. The compositions are dormant and become active upon exposure to hydration and oxygen. 2. The antimicrobial composition of claim 1 , wherein said activity is bacteriostatic or bacteriocidal.3. The antimicrobial composition of claim 1 , wherein said activity is viricidal.4. The antimicrobial composition of claim 1 , wherein said activity is fungicidal.6. The liquid antimicrobial composition of claim 5 , wherein said first component further comprises a hydrogen peroxide producing enzyme and said hydrogen peroxide source is a substrate for said hydrogen peroxide producing enzyme7. The antimicrobial composition of claim 5 , wherein said activity is bacteriostatic or bacteriocidal.8. The antimicrobial composition of claim 5 , wherein said activity is viricidal.9. The antimicrobial composition of claim 5 , wherein said activity is fungicidal.10. The antimicrobial composition of claim 1 , wherein said first and second components are layers.11. The antimicrobial composition of claim 10 , wherein one of said layers is internal to the other said layer.12. The antimicrobial composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles have an iron oxide composition.13. The antimicrobial composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles have a magnetic nanoparticle size distribution in which at least 90% of magnetic nanoparticles have a size of at least 3 nm and up to 30 nm claim 1 , and an aggregated particle size distribution in which at least 90% of said mesoporous ...

Rna affinity purification

Provided herein, in some embodiments, are methods of purifying a nucleic acid preparation. The methods may comprise contacting a nucleic acid preparation comprising messenger ribonucleic acid with an RNase III enzyme that is immobilized on a solid support and binds to double-stranded RNA contaminants.

Probiotic bacteria comprising metals, metal nanoparticles and uses thereof

The invention relates to probiotic bacteria selected from lactic acid bacteria, such as Lactobacillus and Bifidobacteria, having metals and/or metal nanoparticles and to foodstuff and pharmaceutical composition having these bacteria. The invention also provides methods for obtaining these bacteria and uses of these bacteria for the treatment and prevention of mineral deficiency pathologies, as a contrast agent for the imaging of the digestive tract and for the treatment of cancer.

BIOFUNCTIONAL MATERIALS

Provided are methods and compositions for self-cleaning that include a digestive protein capable of decomposing stain forming molecules, a substrate applied to a solid surface, and a linker moiety bound to an outer surface of said substrate and an active group of said digestive protein, said linker moiety between said protein and said substrate and covalently linking said protein to a surface of said substrate by an amide bond, the linker moiety between a free amine of said protein and said outer surface of said substrate wherein the digestive protein forms a layer on a surface of said substrate such that the digestive protein is surface exposed for reaction with a stain. 1. A process for self-cleaning , comprising:binding a digestive protein to the surface of a substrate, the digestive protein capable of decomposing a stain forming molecule;the digestive protein bound to the surface by a linker moiety between an active group of the digestive protein and said substrate, said active group comprising a free amine; andthe digestive protein forming a layer on the surface such that the digestive protein is surface exposed for self-cleaning of a stain comprising one or more of said stain forming molecules.2. The process according to claim 1 , wherein said substrate comprises one or more selected from the group consisting of alcohol claim 1 , thiol claim 1 , aldehyde claim 1 , carboxylic acid claim 1 , anhydride claim 1 , epoxy claim 1 , and ester.3. The process according to claim 1 , wherein said surface is selected from the group consisting of metal claim 1 , glass claim 1 , paint claim 1 , plastic claim 1 , and fabrics.4. The process according to claim 1 , wherein said free amine is a lysine claim 1 , arginine claim 1 , asparagine claim 1 , glutamine claim 1 , or an N-terminal end.5. The process according to claim 1 , wherein the self-cleaning of a stain molecule by said digestive protein occurs in a dry environment.6. The process of claim 5 , wherein an end product of ...

BIOFUNCTIONAL MATERIALS

Provided are methods for providing thermal stability to a protein whereby a digestive protein is covalently bound to a substrate by a linker moiety bound to an outer surface of said substrate and an active group of the protein, the linker moiety covalently linking the protein to a surface of the substrate wherein the digestive protein forms a layer on the surface of said substrate such that the digestive protein is surface exposed. 1. A process for stabilizing a protein against thermal inactivation , comprising:binding a protein to the surface of a solid substrate;the protein bound to the surface by a linker moiety between an active group of the protein and said substrate such that said binding of the protein to the solid substrate stabilizes the protein against thermal inactivation.2. The process of wherein the protein forms a layer on the surface such that the protein is surface exposed.3. The process according to claim 1 , wherein said substrate comprises one or more selected from the group consisting of alcohol claim 1 , thiol claim 1 , aldehyde claim 1 , carboxylic acid claim 1 , anhydride claim 1 , epoxy claim 1 , and ester.4. The process according to claim 1 , wherein said surface is selected from the group consisting of metal claim 1 , glass claim 1 , paint claim 1 , plastic claim 1 , and fabrics.5. The process according to claim 1 , wherein the digestive protein is bound to the substrate via a free amine on the protein.6. The process according to wherein said free amine is a lysine claim 5 , arginine claim 5 , asparagine claim 5 , glutamine claim 5 , or an N-terminus.7. The process according to claim 1 , wherein the protein is a lysozyme claim 1 , protease claim 1 , lipase claim 1 , cellulase claim 1 , glycosidase claim 1 , or amylase.8. The process according to wherein the binding is performed by spin coating the protein onto the surface.9. The process according to wherein the surface comprises polystyrene.10. The process according to wherein the binding ...

BIOFUNCTIONAL MATERIALS

The present disclosure relates to compositions and processes in the field of self-cleaning system using digestive proteins. One composition includes a substrate, a digestive protein capable of decomposing a stain molecule, and a linker moiety bound to both said digestive protein and said substrate. The processes include binding a substrate to a surface and forming a linker moiety between a digestive protein and said substrate. 1. A composition for removing a stain from a solid surface comprising:a digestive protein capable of decomposing stain forming molecules;a substrate applied to a solid surface; anda linker moiety bound to an outer surface of said substrate and an active group of said digestive protein, said linker moiety between said protein and said substrate and covalently linking said protein to the surface of the substrate;{'sup': −3', '2, 'said digestive protein forming a layer on a surface of said substrate such that the digestive protein is covalently attached on the surface of the substrate with an activity in excess of 0.6×10unit/cmand wherein the digestive protein is surface exposed for reaction with a stain molecule.'}2. The composition according to claim 1 , wherein the digestive protein is selected from the group consisting of lysozymes claim 1 , proteases claim 1 , lipases claim 1 , cellulases claim 1 , glycosidases claim 1 , amylases claim 1 , and combinations thereof.3. The composition according to claim 1 , wherein said stain molecule comprises protein claim 1 , oil claim 1 , fat claim 1 , carbohydrate claim 1 , or combinations thereof.4. The composition according to claim 1 , wherein said substrate comprises one or more surface active groups selected from alcohol claim 1 , thiol claim 1 , aldehyde claim 1 , carboxylic acid claim 1 , anhydride claim 1 , epoxy claim 1 , and ester.5. The composition according to claim 4 , wherein said surface active group forms an amide bond to said digestive protein.6. The composition according to claim 1 , ...

IMMOBILIZED CYCLOALIPHATIC PEPTIDE ACYLTRANSFERASE AND PREPARATION METHOD AND USES THEREOF

Disclosed in the present invention are an immobilized cycloaliphatic peptide acyltransferase and a preparation method and use thereof. The cycloaliphatic peptide acyltransferase is immobilized on a carrier; the cycloaliphatic peptide acyltransferase is derived from natural or artificial mutants or variants thereof, or can be obtained by introducing a foreign cyclic acyltransferase gene and transforming thereafter; the material of the carrier is selected from an inorganic carrier or a polypropylene resin carrier. Also disclosed in the present invention are the preparation method for the immobilized cycloaliphatic peptide acyltransferase and uses thereof. 122-. (canceled)24. The method according to claim 23 , wherein the ratio of the immobilized cycloaliphatic peptide acyltransferase to the compound of formula I is 0.01-10 u/g.25. The method according to claim 23 , wherein the pH value of the buffer in step (a) is 4-9.26. The method according to claim 23 , wherein the buffer in step (a) is selected from one or more of the group consisting of sodium citrate buffer claim 23 , potassium dihydrogen phosphate-disodium hydrogen phosphate buffer and Tris-HCl buffer.27. The method according to claim 23 , wherein the temperature for deacylation reaction in step (b) is 20-70° C.28. The method according to claim 23 , wherein claim 23 , in step (b) claim 23 , upon deacylation claim 23 , the immobilized cycloaliphatic peptide acyltransferase is separated from the product-containing reaction solution to give the compound of formula II.29. The method according to claim 28 , wherein the method for separating the immobilized cycloaliphatic peptide acyltransferase from the product-containing reaction solution includes filtration or centrifugation. The present invention relates to enzyme immobilization, particularly to an immobilized cycloaliphatic peptide acyltransferase and preparation method and uses thereof.Echinocandins, as a new class of antifungal agents, exhibit good effects in ...

Apparatus for the extracorporeal treatment of blood

An apparatus for the extracorporeal treatment of blood comprising an extracorporeal blood circuit ( 2 ), a pump ( 6 ) configured to provide fluid displacement within the extracorporeal blood circuit, and a reaction chamber ( 8 ) connected to the extracorporeal blood circuit and configured to receive blood or plasma from the circuit and treat the blood or plasma. The reaction chamber comprises an protease enzyme immobilized to a support, in which the protease enzyme is specific for, and capable of irreversibly cleaving, a human C5a present in the blood or plasma, wherein the abundance of the human C5a in the treated blood or plasma is less than that in the untreated blood or plasma. The apparatus finds utility in the extracorporeal treatment of blood from patients with inflammatory conditions, especially auto-immune disease and sepsis.

PRINTABLE MAGNETIC POWDERS AND 3D PRINTED OBJECTS FOR BIONANOCATALYST IMMOBILIZATION

The invention provides materials, and in particular, magnetic materials, for the universal immobilization of enzymes and enzyme systems. Described herein are highly magnetic and highly porous composite blends of thermoplastics with magnetic particles to form powders, single-layered, or multiple-layered materials that are used as scaffolds for magnetically immobilized enzymes known as bionanocatalysts (BNCs). Designed objects are produced using 3D printing by sintering composite magnetic powders. In some embodiments, Selective Laser Sintering (SLS) is used. The invention provides the use of the material compositions for 3D printing of enzyme supports and flow cells allowing continuous production of, e.g., small molecules. 1. A magnetic macroporous powder , comprising a thermoplastic polymer and magnetic microparticles , wherein said powder is operative for additive manufacturing (AM) of a shaped magnetic macroporous scaffold for immobilizing self-assembled mesoporous aggregates of magnetic nanoparticles.2. The magnetic macroporous powder of claim 1 , wherein said magnetic particles have a size of between about 50-100 μm.3. The magnetic macroporous powder of claim 1 , wherein said magnetic particles have a size of between about 10-50 μm.4. The magnetic macroporous powder of claim 1 , wherein said magnetic particles have a size of between about 5-10 μm.5. The magnetic macroporous powder of claim 1 , wherein said magnetic particles have a size of about 10 μm.6. The magnetic macroporous powder of claim 1 , wherein said magnetic particles have a size of about 5 μm.7. The magnetic macroporous powder of claim 1 , wherein said magnetic particles have a size of less than 5 μm.8. The magnetic macroporous powder of claim 1 , wherein said magnetic particles have a size of greater than 100 μm.9. The magnetic macroporous powder of has an average size of about 150 μm.10. The magnetic macroporous powder of has an average size of about 75 μm.11. The magnetic macroporous powder of has ...