Гиспидин-синтазы и их применение

Группа изобретений относится к области биотехнологии, конкретно к белкам биосинтеза люциферина грибов, представляющим собой гиспидин-синтазу. Предложены новые гиспидин-синтазы, кодирующие их нуклеиновые кислоты, кассеты экспрессии и клетки-хозяевы для получения указанных ферментов, применение гиспидин-синтаз в биосинтезе люциферина грибов и в способе биосинтеза предлюциферина грибов в системах in vitro и in vivo. Изобретения обеспечивают эффективный способ синтеза гиспидина и люциферина грибов и могут быть использованы для получения автономных биолюминесцентных систем, обладающих видимым свечением. 7 н. и 12 з.п. ф-лы, 9 ил., 6 табл., 25 пр.

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Изобретение раскрывает способ крупномасштабного получения антител против амилоидного белка бета в культуре клеток. В способе используют питательную среду для культивирования клеток, трансформированных геном, кодирующим целевой продукт. Одной из особенностей питательной среды является следующий признак: суммарная концентрация аминокислот больше, чем приблизительно 70 мМ; отношение суммарного молярного количества глютамина к суммарному количеству аспарагина меньше, чем приблизительно 2; отношение суммарного молярного количества глютамина к суммарному количеству всех аминокислот меньше, чем приблизительно 0.2; отношение суммарного молярного количества неорганических ионов к суммарному количеству всех аминокислот составляет приблизительно от 0.4 до 1 или совокупное суммарное количество глютамина и аспарагина на единицу объема находится в диапазоне приблизительно от 16 мМ до 36 мМ. Такая система культивирования обеспечивает высокие уровни продуцирования антител. Способы культивирования могут ...

ОБЪЕКТ СОИ 9582.814.19.1, ПРИДАЮЩИЙ УСТОЙЧИВОСТЬ К НАСЕКОМЫМ И УСТОЙЧИВОСТЬ К ГЕРБИЦИДАМ

Изобретение относится к области биохимии, в частности к способу борьбы с чешуекрылыми насекомыми. Также раскрыта выделенная нуклеиновая последовательность, которая является праймером. Раскрыты растение сои, которое устойчиво к Pseudoplusia includens (соевой пяденице), семя для выращивания указанного растения сои и часть растения сои, которая устойчива к Pseudoplusia includens (соевой пяденице). Изобретение позволяет эффективно бороться с чешуекрылыми насекомыми. 5 н. и 2 з.п. ф-лы, 3 ил., 8 табл., 7 пр.

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Настоящее изобретение относится к биохимии. Культивируют содержащие покоящийся центр ткани корня растения в среде, содержащей 2,4-D. Отбирают белую недифференцированную ткань из культивируемой ткани, где ткань представляет собой клеточную линию. Указанная клеточная линия характеризуется следующим: в суспензионной культуре находится в одноклеточном состоянии, морфологически ее ядра больше, чем в клеточных линиях корня того же растения, окружена слизистыми веществами, растет стабильно и дольше по сравнению с клеточными линиями корня того же растения, демонстрирует высокую жизнеспособность при криоконсервации. Изобретение позволяет получать генетически стабильные клеточные линии для получения полезных веществ с помощью культур растительных клеток. 2 н. и 2 з.п. ф-лы, 11 ил., 1 табл., 3 пр.

СПОСОБЫ IN VITRO ДЛЯ СОЗДАНИЯ И ПОДДЕРЖАНИЯ ЛИНИЙ РАСТИТЕЛЬНЫХ КЛЕТОК В ВИДЕ ОТДЕЛЬНЫХ КЛЕТОК В СУСПЕНЗИИ С ИНТАКТНЫМИ КЛЕТОЧНЫМИ СТЕНКАМИ И ИХ ТРАНСФОРМАЦИИ

Изобретение относится к биотехнологии. Описан способ получения отдельной растительной клетки, где способ включает: помещение агрегированных растительных клеток, содержащих интактные клеточные стенки, в среду, содержащую глицерин и разделяющее средство, выбранное из группы, состоящей из фермента, расщепляющего пектин, и соединения, деполимеризующего тубулин; встряхивание агрегированных растительных клеток со стадии (а) при температуре 25-28°С в темноте на орбитальном шейкере при 150 об/мин и перенос одной или нескольких растительных клеток, содержащих интактные клетки, в среду культивирования растения, которая не содержит глицерин и разделяющее средство, выбранное из группы, состоящей из фермента, расщепляющего пектин, и соединения, деполимеризующего тубулин. Раскрыт способ трансформации клетки, полученной описанным способом, при этом указанный способ включает получение изолированной клетки согласно описанному способу, трансформацию указанной клетки гетерологичным полинуклеотидом и отбор ...

КЛЕТОЧНЫЕ КЛОНЫ КАМБИЯ, СПОСОБ ИХ ВЫДЕЛЕНИЯ И КОНСЕРВАЦИИ

Ткань растения, содержащую камбий, культивируют, индуцируя слой камбия и каллус из других тканей. Из полученной смеси выделяют и пролиферируют клон одной клетки камбия. Создание гомогенной клеточной линии из камбия позволяет минимизировать варьирование скорости роста клеток и образования вторичных метаболитов. Тем самым обеспечивается стабильность производства биологически активных веществ растительного происхождения в ходе долгосрочного культивирования. 4 н. и 11 з.п. ф-лы, 5 ил., 5 табл.

ФУНКЦИОНАЛЬНЫЕ ЛОКУСЫ FAD2 И СООТВЕТСТВУЮЩИЕ СПЕЦИФИЧНЫЕ ДЛЯ САЙТА-МИШЕНИ СВЯЗЫВАЮЩИЕСЯ БЕЛКИ, СПОСОБНЫЕ ИНДУЦИРОВАТЬ НАПРАВЛЕННЫЕ РАЗРЫВЫ

Изобретение относится к области биохимии, в частности к способу интегрирования интересующей последовательности нуклеиновой кислоты в ген FAD2 в клетку сои, включающему расщепление сайт-специфическим образом гена FAD2 клетки сои с использованием нуклеазы с «цинковыми пальцами». Также раскрыта сайт-специфическая нуклеаза с «цинковыми пальцами» для использования с целью модификации гена FAD2. Изобретение позволяет эффективно интегрировать интересующую последовательность нуклеиновой кислоты в клетку растения сои. 2 н. и 4 з.п. ф-лы, 8 ил., 5 табл., 5 пр.

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Изобретение относится к области биохимии, в частности к способу введения сиквенс-специфичной нуклеазы (ССН) в растительную клетку. При этом способ включает обеспечение наличия растительной клетки, имеющей клеточную стенку; покрытие поверхности наночастицы ССН; приведение растительной клетки, имеющей клеточную стенку, и покрытой наночастицы в контакт друг с другом; и обеспечение поглощения наночастицы и ССН в растительную клетку, содержащую клеточную стенку. Изобретение позволяет эффективно осуществлять введение ССН в растительную клетку, имеющую клеточную стенку. 14 з.п. ф-лы, 7 ил., 5 пр.

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Изобретение относится к косметической промышленности и представляет собой косметическое применение дедифференцированных растительных клеток из растения рода Rosa sp., лиофилизата указанных клеток или экстракта указанных клеток, полученного в водном или водорастворимом растворителе или в их смесях, в качестве активного агента для ухода за стареющей кожей для лечения эстетического дефекта, являющегося следствием недостатка в обновлении тканей кожи, и/или для сокращения, и/или лечения признаков старения кожи, которые выбраны из группы, состоящей из: истончения кожи, потери упругости, потери эластичности, потери плотности или потери тонуса кожи, сухости кожи, изменения внешнего вида поверхности кожи, появления заметного микрорельефа кожи, появления шероховатости, формирования и/или наличия тонких линий и/или морщин, изменения интенсивности цвета кожи лица, морщинистого вида кожи, дряблости кожи или увядания кожи. Изобретение обеспечивает эффективное восстановление регенерационной способности ...

БЛОК КЛЕТОЧНЫХ ЯЧЕЕК МИКРОФЛЮИДНОГО ЧИПА

Полезная модель относится к области биохимии и молекулярной биологии, а именно к конструкции микрофлюидного чипа, который может быть использован для культивирования и/или исследования клеток человека, животных, растений и/или культур вирусов. В частности, полезная модель представляет собой усовершенствованную часть микрофлюидного чипа, характеризующую выполнение, по меньшей мере, двух клеточных ячеек с пробками для герметизации полостей ячеек.Блок клеточных ячеек микрофлюидного чипа включает клеточные ячейки, входящие в микрофлюидную систему чипа заданной топологии, снабженные пробками, выполненными с возможностью герметизации полостей ячеек, где пробки выполнены объединенными жесткой связью с образованием единой монолитной детали, при этом пробки образованы выступами, сформированными на внутренней стороне упомянутой детали, контактирующей с соответствующей поверхностью чипа. Количество, форма и взаимное расположение данных выступов соответствуют количеству ячеек, форме верхней части их ...

СПОСОБ ПОВЫШЕНИЯ ЭФФЕКТИВНОСТИ КУЛЬТИВИРОВАНИЯ КАЛЛУСНОЙ КУЛЬТУРЫ ЛИМОННИКА КИТАЙСКОГО (SCISANDRA CHINENSIS (TURCZ.) BAILL.) В УСЛОВИЯХ IN VITRO

Изобретение относится к области биотехнологии и может быть использовано в пищевой и фармацевтической промышленности для культивирования каллусной культуры лимонника китайского (Schisandra chinensis (Turcz.) Baill.). Способ культивирования каллусной культуры лимонника китайского {Schisandra chinensis (Turcz.) Baill.) включает стерилизацию посадочного материала, приготовление питательной среды на минеральной основе Мурасиге-Скуга (MS) или Гамборга (В-5) с добавлением или без гормонов - 6-бензиламинопурина (6-БАП) и нафтилуксусной кислоты (НУК), культивирование в стерильных условиях при фотопериоде (свет/темнота) 16/8 часов, температуре 25±2°C и влажности 60-70%. Техническим результатом, полученным при реализации заявленного способа, является получение биомассы каллусной культуры лимонника китайского с большим выходом и высоким содержанием биологически активных веществ фенольной природы. 3 з.п. ф-лы, 3 табл.

УСТРОЙСТВО ДЛЯ БИОПЕЧАТИ ОДИНОЧНЫМИ ТКАНЕВЫМИ СФЕРОИДАМИ И ИСПОЛЬЗУЕМАЯ В НЕМ ПЕЧАТАЮЩАЯ ГОЛОВКА

Группа изобретений относится к области биотехнологии. Предложена печатающая головка и устройство печати тканевыми сфероидами. Печатающая головка включает систему каналов, содержащую входной и выходной каналы, верхний и нижний каналы сепарации питательной среды, а также канал для подпирающего плунжера. Входной канал имеет диаметр, обеспечивающий перемещение сфероида по нему только по одному. Выходной канал имеет диаметр не меньше диаметра входного канала, включает входное отверстие для ввода печатающего инструмента и выходное отверстие для вывода сфероидов. Каналы сепарации выполнены с возможностью соединения приспособления для отвода питательной среды с выходным каналом через систему микроканалов. Устройство включает печатающую головку, устройство подачи сфероидов с питательной средой во входной канал, приспособление для отвода питательной среды, подпирающий плунжер, печатающий инструмент, систему регистрации положения сфероида в выходном канале и вычислительное устройство для управления ...

ЛОКУСЫ ФУНКЦИОНАЛЬНОСТИ FAD2 И СООТВЕТСТВУЮЩИЕ СПЕЦИФИЧНЫЕ К УЧАСТКУ-МИШЕНИ СВЯЗЫВАЮЩИЕ БЕЛКИ, СПОСОБНЫЕ ИНДУЦИРОВАТЬ НАПРАВЛЕННЫЕ РАЗРЫВЫ

... 1. Способ модификации генома клетки, причем способ включает:сайт-специфическое расщепление участка-мишени в гене FAD2 клетки, тем самым внося разрыв в ген FAD2;где ген FAD2 модифицируют после расщепления.2. Способ по п. 1, где способ дополнительно включает встраивание в разрыв представляющей интерес последовательности нуклеиновой кислоты.3. Способ по п. 1 или 2, где ген FAD2 представляет собой ген FAD2A, FAD2A', FAD2C и/или FAD2C'.4. Способ по п. 1 или 2, где сайт-специфическое расщепление включает введение слитого белка, содержащего ДНК-связывающий домен и домен расщепления или половинный домен расщепления в клетку, или полинуклеотида, кодирующего слитый белок, где слитый белок специфически связывается с участком-мишенью и осуществляет расщепление в участке-мишени или вблизи участка-мишени, тем самым внося разрыв.5. Способ по п. 4, где ДНК-связывающий домен выбран из группы, состоящей из ДНК-связывающего домена мегануклеазы, ДНК-связывающего домена лейциновой молнии, ДНК-связывающий домен ...

КОМПОЗИЦИИ И СПОСОБЫ ДЛЯ БОРЬБЫ С ФУЗАРИОЗОМ

Группа изобретений относится к биотехнологии и сельскому хозяйству. Предложены выделенный штамм вида Variovorax sp. NRRL В-50469, обладающий подавляющей активностью в отношении фузариоза, композиции и способы с использованием указанного штамма. Способ предотвращения развития фузариоза включает выращивание микробного штамма или его культуры в среде для роста или почве растения. Способ обработки против развития фузариоза включает нанесение на растение или на окружающую его среду эффективного количества микробного штамма или его культуры. Также предложены композиции для подавления фузариоза, содержащие микробный штамм или его культуру, семя, резистентное к инфекции грибка Fusarium, покрытое составом для покрытия семян, содержащим композицию. Изобретения эффективны в борьбе с фузариозом злаковых растений, таких как пшеница и ячмень. 8 н. и 10 з.п. ф-лы, 7 табл., 11 пр.

СПОСОБ ПОЛУЧЕНИЯ ЭКСТРАКТОВ ИЗ РОДА PANAX, ВКЛЮЧАЯ ДИКИЙ ЖЕНЬШЕНЬ ИЛИ ЖЕНЬШЕНЬ, ЛИБО КАМБИАЛЬНЫХ МЕРИСТЕМАТИЧЕСКИХ КЛЕТОК, ПРОИСХОДЯЩИХ ИЗ РОДА PANAX, ИЛИ ИХ ЭКСТРАКТОВ, СОДЕРЖАЩИХ РЕДКИЕ ГИНСЕНОЗИДЫ В БОЛЬШОМ КОЛИЧЕСТВЕ

Изобретение относится к области биотехнологии. Изобретение относится к способу повышения содержания гинсенозида Rh2 и одного или нескольких гинсенозидов, выбранных из группы, состоящей из Rg3, Rk1, Rg5, Rk2, Rh3 и PPD, в камбиальных меристематических клетках (CMCs) из рода Panax, который включает тепловую обработку культивируемых камбиальных меристематических клеток (CMCs) из рода Panax при температуре от 85°C до 160°C, камбиальным меристематическим клеткам (CMCs) из рода Panax, в которых содержание гинсенозида Rh2 и одного или нескольких гинсенозидов, выбранных из группы, состоящей из Rg3, Rk1, Rg5, Rk2, Rh3 и PPD, повышено по сравнению с их содержанием до тепловой обработки с помощью способа по п. 1, экстракту женьшеня, в котором содержание гинсенозида Rh2 и одного или нескольких гинсенозидов, выбранных из группы, состоящей из Rg3, Rk1, Rg5, Rk2, Rh3 и PPD, повышено по сравнению с их содержанием до тепловой обработки с помощью способа по п. 2, применению камбиальных меристематических ...

ИНСЕКТИЦИДНЫЕ ПОЛИПЕПТИДЫ С ШИРОКИМ СПЕКТРОМ АКТИВНОСТИ И ИХ ПРИМЕНЕНИЯ

РЕГУЛЯТОРНЫЕ ЭЛЕМЕНТЫ РАСТЕНИЙ И СПОСОБЫ ИХ ПРИМЕНЕНИЯ

ОПОСРЕДОВАННАЯ НАНОЧАСТИЦАМИ ДОСТАВКА СИКВЕНС-СПЕЦИФИЧНЫХ НУКЛЕАЗ

... 1. Способ введения сиквенс-специфичной нуклеазы (ССН) в растительную клетку, где способ включает:обеспечение наличия растительной клетки, имеющей клеточную стенку;покрытие поверхности наночастицы ССН с помощью подхода, выбранного из группы,состоящей из электростатической адсорбции, конъюгации с лигандом на поверхности наночастицы, покрытия наночастицы малым кофактором, который ССН может распознать и связать, и непосредственной конъюгации с наночастицей;приведение растительной клетки, имеющей клеточную стенку, и покрытой наночастицы в контакт друг с другом; иобеспечение поглощения наночастицы и ССН в растительную клетку, содержащую клеточную стенку.2. Способ по п. 1, в котором покрытие наночастицы ССН включает иммобилизацию ССН за счет нековалентной адсорбции на поверхности наночастицы.3. Способ по п. 1, который дополнительно включает поглощение ССН внутрь наночастицы.4. Способ по п. 1, который дополнительно включает обеспечение поглощения наночастицы компартментом растительной клетки, имеющей ...

КОМБИНАЦИИ, ВКЛЮЧАЮЩИЕ БЕЛКИ Cry34Ab/35Ab И Сry3Ba, ДЛЯ ПРЕДОТВРАЩЕНИЯ РАЗВИТИЯ УСТОЙЧИВОСТИ У КУКУРУЗНЫХ КОРНЕВЫХ ЖУКОВ (Diabrotica spp.)

... 1. Трансгенное растение, которое продуцирует белок Cry34, белок Cry35 и инсектицидный белок Cry3B.2. Трансгенное растение по п.1, где указанное растение дополнительно продуцирует четвертый инсектицидный белок, выбранный из группы, состоящей из Cry3A и Cry6A.3. Семя растения по любому из пп.1 и 2, где указанное семя содержит указанную ДНК.4. Поле растений, содержащее множество растений по любому из пп.1 и 2.5. Поле растений по п.4, где указанное поле дополнительно содержит резервные растения, не содержащие Bt белки, где указанные резервные растения составляют менее чем 40% всех культур на указанном поле.6. Поле растений по п.5, где указанные резервные растения составляют менее чем 30% всех культур на указанном поле.7. Поле растений по п.5, где указанные резервные растения составляют менее чем 20% всех культур на указанном поле.8. Поле растений по п.5, где указанные резервные растения составляют менее чем 10% всех культур на указанном поле.9. Поле растений по п.5, где указанные резервные ...

СПОСОБЫ И КОМПОЗИЦИИ ДЛЯ УСТОЙЧИВОСТИ РАСТЕНИЙ К ГЕРБИЦИДАМ

СПОСОБ СТИМУЛЯЦИИ МОРФОГЕНЕЗА В КУЛЬТУРЕ ТКАНИ ЯЧМЕНЯ

КОМБИНАЦИИ, ВКЛЮЧАЮЩИЕ БЕЛКИ Cry34Ab/35Ab И Cry6Aa, ДЛЯ ПРЕДОТВРАЩЕНИЯ РАЗВИТИЯ УСТОЙЧИВОСТИ У КУКУРУЗНЫХ КОРНЕВЫХ ЖУКОВ (Diabrotica spp.)

... 1. Трансгенное растение, которое продуцирует белок Cry34, белок Cry35 и инсектицидный белок Cry6A.2. Трансгенное растение по п.1, где указанное растение дополнительно продуцирует четвертый инсектицидный белок, выбранный из группы, состоящей из Cry3B и Cry3A.3. Семя растения по любому из пп.1 или 2, где указанное семя содержит указанную ДНК.4. Поле растений, содержащее множество растений по любому из пп.1 или 2.5. Поле растений по п.4, где указанное поле дополнительно содержит резервные растения, не содержащие белки Bt, где указанные резервные растения составляют менее чем 40% всех культур на указанном поле.6. Поле растений по п.5, где указанные резервные растения составляют менее чем 30% всех культур на указанном поле.7. Поле растений по п.5, где указанные резервные растения составляют менее чем 20% всех культур на указанном поле.8. Поле растений по п.5, где указанные резервные растения составляют менее чем 10% всех культур на указанном поле.9. Поле растений по п.5, где указанные резервные ...

КОМБИНАЦИИ, ВКЛЮЧАЮЩИЕ БЕЛКИ Cry3Aa И Cry6Aa, ДЛЯ ПРЕДОТВРАЩЕНИЯ РАЗВИТИЯ УСТОЙЧИВОСТИ У КУКУРУЗНЫХ КОРНЕВЫХ ЖУКОВ (Diabrotica spp.)

... 1. Трансгенное растение, которое продуцирует инсектицидный белок Cry3A и инсектицидный белок Cry6A.2. Трансгенное растение по п.1, где указанное растение дополнительно продуцирует третий инсектицидно активный белок, выбранный из группы, состоящей из Cry3B, Cry34 и Cry35.3. Семя растения по любому из пп.1-2, где указанное семя содержит указанную ДНК.4. Поле растений, содержащее множество растений по любому из пп.1-2.5. Поле растений по п.4, где указанное поле дополнительно содержит резервные не содержащие Bt белки растения, где указанные резервные растения составляют менее чем 40% всех культур на указанном поле.6. Поле растений по п.5, где указанные резервные растения составляют менее чем 30% всех культур на указанном поле.7. Поле растений по п.5, где указанные резервные растения составляют менее чем 20% всех культур на указанном поле.8. Поле растений по п.5, где указанные резервные растения составляют менее чем 10% всех культур на указанном поле.9. Поле растений по п.5, где указанные резервные ...

КОМПОЗИЦИИ И СПОСОБЫ ДЛЯ БОРЬБЫ С ФУЗАРИОЗОМ

... 1. Выделенный микробный штамм, выбранный из группы, состоящей из штамма вида Microbacterium, штамма вида Bacillus, штамма вида Mycosphaerella и штамма вида Variovorax, где указанный микробный штамм обладает супрессорной активностью против фузариоза.2. Выделенный микробный штамм по п.1, где указанный микробный штамм выбирают из группы, состоящей из штамма SGI-010-H11 вида Mycosphaerella (депозит NRRL 50471), штамма SGI-014-C06 вида Microbacterium (депозит NRRL B-50470), штамма SGI-014-G01 вида Variovorax (депозит NRRL B-50469), штамма SGI-015-F03 вида Bacillus (депозит NRRL B-50760), штамма SGI-015-H06 вида Bacillus (депозит NRRL B-50761) и их обладающих пестицидной активностью вариантов.3. Выделенный микробный штамм по п.2, где указанный микробный штамм содержит последовательность ДНК, демонстрирующую по меньшей мере 85% идентичность любой из нуклеотидных последовательностей, приведенных в Перечне последовательностей.4. Биологически чистая культура микробного штамма по п.1.5. Обогащенная ...

КОСМЕТИЧЕСКОЕ ПРИМЕНЕНИЕ ДЕДИФФЕРЕНЦИРОВАННЫХ РАСТИТЕЛЬНЫХ КЛЕТОК

... 1. Косметическое применение дедифференцированных растительных клеток из растения рода Rosa sp.или экстракта или лиофилизата указанных клеток в качестве активного агента для ухода за стареющей кожей или стареющими волосами.2. Применение по п. 1 для лечения и/или профилактики эстетического дефекта, являющегося результатом недостатка обновления ткани кожи или волос.3. Применение по п. 1 или 2 для предотвращения, и/или сокращения, и/или лечения признаков старения кожи.4. Применение по п. 3, при котором признаки старения кожи выбраны из следующих: истончение кожи, потеря упругости, потеря эластичности, потеря плотности или потеря тонуса кожи, сухость кожи, изменение внешнего вида поверхности кожи, появление заметного микрорельефа кожи, появление шероховатости, формирование и/или наличие тонких линий и/или морщин, изменение интенсивности цвета кожи лица, морщинистый вид кожи, изменение запаха кожи, дряблость кожи или увядание кожи.5. Применение по п. 1 или 2 для предотвращения, и/или сокращения ...

СПОСОБЫ IN VITRO ДЛЯ СОЗДАНИЯ И ПОДДЕРЖАНИЯ ЛИНИЙ РАСТИТЕЛЬНЫХ КЛЕТОК В ВИДЕ ОТДЕЛЬНЫХ КЛЕТОК В СУСПЕНЗИИ С ИНТАКТНЫМИ КЛЕТОЧНЫМИ СТЕНКАМИ И ИХ ТРАНСФОРМАЦИИ

... 1. Изолированная отдельная растительная клетка, содержащая интактную клеточную стенку. ! 2. Способ получения клетки по п.1, в котором указанный способ включает в себя культивирование растительной клетки, содержащей интактную клеточную стенку, в среде, содержащей глицерин и разделяющее средство, выбранное из группы, состоящей из фермента, расщепляющего пектин, и соединения, деполимеризующего тубулин. ! 3. Способ по п.2, в котором указанная среда является жидкой средой, и указанные клетки находятся в суспензии. ! 4. Способ по п.2, в котором указанное разделяющее средство представляет собой соединение, деполимеризующее тубулин. ! 5. Способ по п.4, в котором указанное соединение, деполимеризующее тубулин, выбрано из группы, состоящей из колхицина и динитроанилина. ! 6. Способ по п.4, в котором указанное соединение, деполимеризующее тубулин соответствует следующей формуле ! ! где ! X = CO2R, CH2CO2R, CH2CH2CO2R, (CH2)3CO2R, OCH2CO2R, OCH(CH3)CO2R, OC(CH3)2CO2R, CH2OCH2CO2R, CH2CH(CO2CH2CH3)CO2R ...

Process for the preparation of rosmarinic acid from plant cell cultures and of plant cell pellets containing rosmarinic acid

Two-stage process for the preparation of rosmarinic acid from plant cell cultures of species of Labiatae and Umbelliferae by growing the plant cells in aqueous nutrient medium in which the production of rosmarinic acid in the plant cells is induced in the 2nd stage by transferring the cells from the maintenance medium into a special production medium and removing the resulting biomasses. The latter can, after drying, be processed directly to pharmaceutical compositions, in particular to tablets which can then be used as medicines.

VERFAHREN ZUR VERBESSERUNG DER GENTRANSFER-EFFIZIENZ IN PFLANZENZELLEN

Transgene Pflanzen mit divergenten SCaM-4 und SCaM-5 Genen zur Etablierung multipler Krankheitsresistenz

PROCESS FOR PROCUCING MATERIAL FOR SMOKING

... 1355570 Cultured tobacco cells JAPAN MONOPOLY CORP 27 Sept 1971 [4 Nov 1970] 44951/71 Heading A2C [Also in Division C6] Processes for producing material for smoking in sheet-like or rod-like form, respectively, involve either of the following sequences of steps, viz. filtering a cultured broth of tobacco cells derived from plants of genus Nicotiana on a 50-120 mesh gauze or filter cloth to form as residue a sheet-like cake having a thickness corresponding to a dried weight of not less than 50 g./m.2 and drying the sheet. like cake, below 80‹C (at least initially), or separating tobacco cells from a cultured broth forming a paste from the cells by adjusting the overall moisture content to 80-93% by weight, casting the paste into a rod shape and freeze-drying the rod-shaped paste. The moisture content of the [separated] tobacco cells may be adjusted by adding limited amounts of fibrous and/or inorganic materials such as wood pulp, hemp, bagasse, beet, tobacco vein, glass fibre, carbon ...

Wheat

A method of producing male-sterile wheat (Triticum) is disclosed comprising: analysing the RNA-transcriptome of wheat stamen cells and pistil cells during the development of the flower, identifying one or more male-fertility wheat (Mfw) genes that at the time of flowering are preferentially expressed in stamens rather than pistils, and inhibiting the expression of the at least one Mfw gene over 50 examples of which are listed at tables 1 and 2. Also disclosed are populations of wheat plants that are predominantly male-sterile as a result of deactivating one or more Mfw genes, processes of obtaining F1 hybrids comprising crossing male-sterile wheat plants with male-fertile wheat, and hybrids produced by these processes. Preferably, the RNA-transcriptome analysis is carried out during meiosis and between stages 41-49 of the Zadoks scale, and the wheat is T. aestivum. Preferably, at least two Mfw genes are inhibited or disrupted, for instance Mfw genes may be deactivated by site-directed mutagenesis ...

Photosynthetic process

CONE OF SEA KALE

A method of production of phytocannabinoids for use in medical treatments

Wheat

Described herein are methods and compositions relating to male-sterile wheat, as well as uses thereof and methods for propagating and maintaining the same.

A process for the preservation of plant embryos.

To improve the storage time of plant embryos, ...

Resistance in plants to infection by ssDNA virus using inoviridae virus ssDNA-binding protein, compositions and methods of use.

The invention describes methods for producing plant resistance to a ssDNA virus, particularly a geminivirus such as mastrevirus, curtovirus or begomovirus. The method comprises introducing a ssDNA-binding protein of the inoviridae virus into the plant, and includes a phage coat protein, particularly, a coliphage gene 5 protein. The invention also describes a transgenic plant comprising a gene that expresses the ssDNA-binding protein and vectors for expressing the protein in plants.

Insect resistant and herbicide tolerant breeding stack of soybean event pDAB9582.814.19.1 and pDAB4468.04.16.1

A PROCESS FOR THE PRESERVATION OF PLANT EMBRYOS

Protein kinases and uses thereof.

Nucleic acid molecules are disclosed that are induced upon pathogen invasion or elicitor treatment. Such molecules aree functional in plants, plant tissue and in plant cells for inducible gene expression and altering the diseasee resistance phenotype of plants. Such molecules are, or are related to, sequences of calcium dependent protein kinase genes. Also diclosed are methods for obtaining transgenic plants containing such nucleic acid molecules and methods for using such molecules. Polypeptides encoded by such nucleic acids are also diclosed herein.

Resistance in plants to infection by ssdna virus using inoviridae virus ssdna-binding protein compositions and methods of use

Reducing levels of nicotinic alkaloids in plants

Method for cellular tissue multiplication from jatropha curcas

Plant stem cell line derived from quiescent centerand method for isolating the same

Plant stem cell line derived from cambium of herbaceous plant with storage root and method for isolating the same

Reducing levels of nicotinic alkaloids in plants

Reducing levels of nicotinic alkaloids in plants

Insect resistant and herbicide tolerant soybean event 9582.814.19.1

Method for cellular tissue multiplication from jatropha curcas.

Plant stem cell line derived from cambium of herbaceous plant with storage root and method for isolating the same

"A process for the preservation of plant embryos".

Improvements brought to the methods of preparation of suspensions of stabilized fresh cells intended for the pharmaceutical, cosmetic or food use.

Insect resistant and herbicide tolerant breeding stack of soybean event pDAB9582.814.19.1 and pDAB4468.04.16.1

Insect resistant and herbicide tolerant soybean event 9582.814.19.1

A PROCESS FOR THE PRESERVATION OF PLANT EMBRYOS

Plant stem cell line derived from quiescent centerand method for isolating the same

MANIPULATION THE PROTOPORPHYRINOGEN OXIDASE ENZYME ACTIVITY INTO EUKARYOTI ORGANISMS

PROCEDURE FOR THE CHROMATOGRAPHI SEPARATION FROM PACLITAXEL AND CEPHALOMANNIN

VERBESSERUNGEN IN ODER BEI DER KULTIVIERUNG VON VERBESSERUNGEN IN ODER BEI DER KULTIVIERUNG VON NADELBÄUMEN NADELBÄUMEN

TRITERPENZUSAMMENSETZUNGEN AND THEIR USE METHODS

PROCEDURE FOR THE ADJUSTMENT OF THE GROWTH FROM PLANTS AND MEANS TO THE EXECUTION OF THE EN OF DRIVING

PROCEDUREPROCEDURE PROCEDURE FOR VEGETABLE CELL VERB END.

AN EFFICIENT METHOD, COTTON FROM CULTIVATED CELLS TO REGENERATING.

PROCEDURE FOR THE PRODUCTION OF PLANT SUBSTANCES.

AGAINST HERBICIDES OF ARYLOXY PHENOXYALKANCARBONSÄURETYP RESISTANT CORN CELLS AND PROCEDURES FOR THE PRODUCTION OF HERBICIDE-RESISTANT CORN CELL LINES

METHODS AND COMPOSITIONS FOR THE PRODUCTION OF STABLY TRANSFORMED ONE, FRUITFUL CORN PLANTS AND CELLS FOR IT

CONTROL OF MALE ONES FERTILITÄT BY USE OF EXTERNALINDUCE-CASH PROMOTER SEQUENCES

INCREASED PRODUCTION OF TAXANEN BY CELL CULTURES OF TAXUS KINDS

RESISTANCE FOR PLANTS IN RELATION TO AN INFECTION WITH A EINZELSTRANG DNS VIRUS, WHICH IS BASED TO ON A EINZELSTRANG DNS BINDING PROTEIN, COMPOSITIONS AND PROCEDURES FOR YOUR USE.

STABILIZED CULTURE FROM ZELLULARAGGREGATEN AND PROCEDURES TO THE DEVELOPMENT OF EMBRYOS FROM PRO EMBRYO GENES TRUNKS FOR THE REGENERATION OF COMMON GRAPE VINES

AGAINST HERBICIDES OF ARYLOXY PHENOXYALKANCARBONSÄURETYP RESISTANT CORN

TRANSGENE PLANTS WITH DIVERGENTEN SCAM-4 AND SCAM-5 GENES FOR THE ESTABLISHMENT OF MULTIPLE DISEASE RESISTANCE

PROCEDURE FOR THE PRODUCTION OF C-4-DEACETYLTAXANEN

FERTILE TRANSGENE CORN PLANTS WITH KIND-STRANGE GENE AS WELL AS PROCEDURES FOR YOUR PRODUCTION

METHODS AND MEDIA FOR ENHANCED SOMATIC EMBRYOGENESIS

A soybean leaf protoplast separation method for subcellular localization

Abstract The invention relates to a soybean leaf protoplast separation method for subcellular localization. The most suitable conditions for isolation of soybean leaf protoplasts are selected by the following factors: adopting the young leaves of Xiangdou No. 3 as materials. In addition, the regulation of the plasmolysis time, the ratios and concentrations of cellulase Onozuka R 10, Hemicellulase, Macerozyme R-10 and Pectolyase Y-23, as well as the regulation of the concentration of mannitol in the the enzymatic hydrolysate, the pH of the enzymatic hydrolysate, and the enzymatic time. The method described in the present invention obtains a large number of free protoplasts with high activity and it is effective for subcellular localization, which is a simple and fast method to isolate the protoplasts of Xiangdou No. 3 leaves.

GENERATION OF COTTON FROM CULTURED CELLS

A method for mass production of taxol by semi-continuous cul ture

Herbicide resistance in plants

High yielding soybean plants with low linolenic acid

METHOD FOR PRODUCING KETOCAROTENOIDS BY CULTIVATING GENETICALLY MODIFIED ORGANISMS

CONTROL OF PLANT CELL PROLIFERATION AND GROWTH

IMPROVEMENTS IN OR RELATING TO PROTEIN PRODUCTION

Insect resistant and herbicide tolerant breeding stack of soybean event

A breeding stack of soybean event 9582.814.19.1 and soybean event pDAB4468.04.16.1 resulted in the novel soybean event pDAB9582.814.19.1 :: pDAB4468.04.16.1. Soybean event pDAB9582.814.19.1 :: pDAB4468.04.16.1 comprises genes encoding AAD-12, CrylF, CrylAc (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.

Enterobacter sp. 638 and methods of use thereof

The present invention relates to a novel species of ...

BULBOUS PLANT TISSUE CULTURE

A method for producing taxol and taxanes from embryo cultures of taxus species

Cotton Variety Sicot 714B3F

H:\tm\Intrwovn\NRPortbl\DCC\TRN\I l106488_l.docx-1/09/2016 The present invention provides a plant of the cotton (Gossypium hirsutum) variety Sicot 714B3F, or a part, cell, tissue or organ thereof.

Glycosylated lysosomal proteins, method of production and uses

The present invention relates to a lysosomal protein composition comprising a plurality of lysosomal proteins that are potentially diversely glycosylated according to a glycosylation pattern, wherein said glycosylation pattern has at least 45 % paucimannosidic N-glycans; a method of manufacturing the lysosomal protein composition in a bryophyte plant or cell, and medical and non-medical uses of the lysosomal protein composition. E.g. the lysosomal protein can be a-Galactosidase for the treatment of Fabry Disease or β-Glucoceramidase for the treatment of Gaucher 's Disease. The unique glycosylation results in improved therapeutic efficacy - surprisingly even without mannose- 6-phosphate that is common for CHO cell produced lysosomal proteins.

Antioxidant, anti-inflammatory, or anti-aging composition containing a plant stem cell line derived from Taxus cambium or procambium as an active ingredient

Compositions and methods for efficient targeting of transgenes

The invention provides recombinant DNA molecules and constructs useful for providing efficient transgene sub-cellular localization of proteins in transgenic plants. Recombinant DNA molecules and constructs for conferring herbicide tolerance or resistance to plants are further provided, as well as plants exhibiting herbicide tolerance and methods for producing or utilizing such plants.

Improvements in or relating to organic compounds

Method of improving gene transfer efficiency into plant cells

STRESS TOLERANT PLANTS AND METHOD FOR THEIR PRODUCTION

PROCESS FOR GENE TRANSFER IN PLANTS

Sulfonylurea-tolerant sunflower plants

Method for identifying plant pathogen tolerance

Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner

A method and device for growing plant, animal or stem cells in a continuous manner.

Supplying treated exhaust gases for effecting growth of phototrophic biomass

There is provided a process for growing a phototrophic biomass in a reaction zone. The process includes treating an operative carbon dioxide supply-comprising gaseous material feed so as to effect production of a carbon dioxide-rich product material. The carbon dioxide concentration of the carbon dioxide-rich product material is greater than the carbon dioxide concentration of the operative carbon dioxide supply-comprising gaseous material feed. Production of at least a fraction of the operative carbon dioxide supply-comprising gaseous material feed is effected by a gaseous exhaust material producing process. At least a fraction of the carbon dioxide-rich product material is supplied to the reaction zone so as to effect growth of the phototrophic biomass by photosynthesis in the reaction zone.

NEW MELON VARIETY NUN 02602 ME

The invention relates to the field of , in particular to a new variety of , NUN 02602 ME, plants, seeds and melon fruits. 1. A seed of melon variety NUN 02602 ME , wherein a representative sample of said seed has been deposited under Accession Number NCIMB ______.2. A plant of melon variety NUN 02602 ME , or a part thereof , wherein a representative sample of seed of said variety has been deposited under Accession Number NCIMB ______.3. A fruit of melon variety NUN 02602 ME claim 2 , or a plant part produced from the plant of .4. A method of producing a melon plant claim 2 , comprising crossing the plant of with a second melon plant one or more times claim 2 , and selecting progeny from said crossing.5. A method of producing a melon plant claim 2 , comprising selfing the plant of one or more times claim 2 , and selecting progeny from said selfing.6. Progeny of melon variety NUN 02602 ME obtained by further breeding with said variety.7. The progeny of claim 6 , wherein said progeny have all the physiological and morphological characteristics of melon variety NUN 02602 ME when grown under the same environmental conditions.8. An Essentially Derived Variety of NUN 02602 ME having one claim 6 , two or three physiological and/or morphological characteristics which are different from those of NUN 02602 ME and which otherwise has all the physiological and morphological characteristics of NUN 02602 ME claim 6 , wherein a representative sample of seed of variety NUN 02602 ME has been deposited under Accession Number NCIMB ______.9. A method of producing plants claim 6 , or a part thereof claim 6 , of variety NUN 02602 ME comprising vegetative propagation of variety NUN 02602 ME.10. The method of claim 9 , wherein said vegetative propagation comprises regenerating a whole plant from a part of variety NUN 02602 ME.11. The method of claim 10 , wherein said part is a cutting claim 10 , a cell culture or a tissue culture.12. A vegetative propagated plant of variety NUN 02602 ME ...

Replication of Undifferentiated Cells in a Weightless Environment, Uses Thereof and a Facility for Such Replication and The Acceleration of the Evolution of Plants and Animals

The present invention provides manufacturing processes for biological replication of undifferentiated plant and animal cells and tissue in a weightless condition, including those systems used in current stem cell research and development and use of undifferentiated parenchyma in plants. The present invention further provides methods for adapting plants and animals to survive outside their native environments. In particular, undifferentiated cells from plants or animals are replicated under weightless conditions in which cell replication or proliferation is accelerated and sustained. Under such conditions, the undifferentiated cells can be “forced” to express sets of genes useful for survival in particular environmental conditions. In this manner, cells surviving prolonged exposure to specific environmental conditions can be selected for and cultivated to produce an organism adapted to that particular environment in an accelerated manner. Methods of identifying specific genes associated with adaptation of a plant or animal to a specific environment are also disclosed. 1. A method of producing at least one undifferentiated cell comprising uniting two haploid cells to form a diploid single cell under conditions to support fertilization in a weightless condition or environment , wherein said single cell can replicate itself forming a diploid cell that will not develop into an embryo of differentiated cells and tissue.2. The method of claim 1 , wherein said haploid cells are gametes claim 1 , and said gametes are a sperm and an egg from an animal or said gametes are a pollen and an ovule from an plant.3. The method of claim 1 , further comprising culturing the diploid single cell to replicate in a weightless condition or environment.4. The method of claim 3 , wherein said method further comprises culturing the diploid single cell to obtain multiple replications of said replicated single cell to produce a suspension of undifferentiated cells.5. The method of claim 4 , ...

Plant Stem Cell Line Derived from Cambium of Herbaceous Plant with Storage Root and Method for Isolating the Same

The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has an active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time. 111-. (canceled)12. A method for isolating a cell line derived from the cambium of an herbaceous plant having a storage root , the method comprising:(a) obtaining storage root tissue of an herbaceous plant having a storage root, wherein the tissue comprises cambium;(b) treating the tissue such that differentiated, non-cambium cells lose the ability to divide;(c) inducing cambium cell proliferation by culturing the obtained cambium-containing storage root tissue in an induction medium comprising a cambium-division hormone; and(d) collecting the induced cambium-derived cell line.13Codonopsis lanceolata, Ostericum koreanumPlatycodon grandiflorum, Pueraria thunbergiana, Aralia contonentalisLedebouriella seseloides, Angelica gigas. The method of claim 12 , wherein the herbaceous plant is selected from the group consisting of ginseng claim 12 , KITAGAWA claim 12 , Kitagawa claim 12 , NAKAI claim 12 , carrot claim 12 , sweet potato claim 12 , Maca claim 12 , and cassava.14. A method for isolating a cell line derived from the cambium of a ginseng root claim 12 , the method comprising:(a) obtaining root tissue of a ginseng plant, wherein the tissue comprises cambium;(b) treating ...

LIQUID-BASED METHOD FOR PRODUCING PLANT EMBRYOS

The present invention relates to methods for developing embryos and producing germination-competent embryos using a liquid embryo development media. 135.-. (canceled)36. A method for determining an optimal settled cell volume for developing plant embryos comprising (1) adding a volume of proliferative plant cells to a volume of liquid embryonic development medium to produce a solution of cells in a vessel , (2) agitating the solution for a first period of time , (3) measuring cell density at least once during and/or after the first period of time , (4) further agitating the solution for a second period of time , (5) recording the number of embryos that have developed in the solution after the second period of time , (6) repeating steps (1) to (5) by adding a different volume of proliferative plant cells to the same volume of liquid embryonic development medium in another vessel and shaking and further shaking the solution for same periods of time , and (7) comparing the recorded numbers of plant embryos that have developed in the solutions , wherein the cell density value for the volume of proliferative plant cells that produces the most number of embryos is an optimal cell density for developing embryos from that species of plant cells.37. The method of claim 36 , wherein the first period of time is about 1-3 weeks.38. The method of claim 36 , wherein the second period of time is about 2 weeks.39. A method for determining an optimal settled cell volume for developing plant embryos comprising (1) adding a volume of proliferative plant cells to a volume of liquid embryonic development medium to produce a solution of cells claim 36 , (2) agitating the solution for a first period of time claim 36 , (3) removing an aliquot of the solution after the first period of time to a vessel claim 36 , (4) allowing the cells in the aliquot of the solution to settle as a layer of cells claim 36 , (5) measuring and recording the height of the cell layer to generate a settled cell ...

METHODS AND DEVICES FOR DISPERSING SOMATIC PLANT EMBRYOS

Methods and devices for dispersion of clusters of somatic plant embryos suspended in a liquid are disclosed. The methods comprise i) subjecting the clusters of embryos to fluid dynamics forces causing axially extensional strain and radially compressional strain and ii) subjecting the clusters of embryos to fluid dynamics forces causing axially compressional strain and radially extensional strain fluid dynamics and iii) repeating said steps in sequence until the individual embryos are separated from each other. The devices may comprise a flow channel including at least one constriction, such that clusters of embryos flowing through the flow channel are first subjected to axially extensional strain and radially compressional strain, and then to axially compressional strain and radially extensional strain from fluid dynamics forces. 1. A method of dispersion of clusters of plant embryos suspended in a liquid into individual plant embryos , said method including at least one dispersion sequence , which comprises the following steps:i) subjecting the clusters of embryos to fluid dynamics forces causing axially extensional strain and radially compressional strain;ii) subjecting the clusters of embryos to fluid dynamics forces causing axially compressional strain and radially extensional strain from fluid dynamics forces; repeating said steps in sequence until the individual embryos are separated from each other.2. The method of claim 1 , wherein the strength of the extensional and compressional strains increases with each repeated sequence.3. A disperser for separating individual somatic plant embryos contained in clusters of embryos claim 1 , comprising a flow channel including at least two constrictions claim 1 ,wherein each constriction has an inner diameter, which is equal to or smaller than the inner diameter of the constriction immediately up-stream of thereof,wherein the flow channel includes an intermediate portion having an essentially constant cross-section, ...

Tomato hybrid px 02481245 and parents thereof

The invention provides seed and plants of tomato hybrid PX 02481245 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of tomato hybrid PX 02481245 and the parent lines thereof, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

COMBINATIONS INCLUDING Cry34Ab/35Ab AND Cry6Aa PROTEINS TO PREVENT DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (DIABROTICA SPP.)

The subject invention relates in part to Cry34Ab/35Ab in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry34Ab/Cry35Ab and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm ( Diabrotica spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. Plants (and acreage planted with such plants) that produce these two insecticidal protein systems are included within the scope of the subject invention. The subject invention also relates in part to combinations of Cry34Ab/35Ab and Cry3Aa proteins “triple stacked” with a Cry6Aa protein. Transgenic plants, including corn, comprising a cry6Aa gene, cry34Ab/35Ab genes, and a cry3Aa gene are included within the scope of the subject invention. Thus, such embodiments target rootworms with three modes of action.

Method of meristem excision and transformation

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

MODIFIED DNA-BINDING PROTEINS AND USES THEREOF

Disclosed herein are enhanced polypeptides, polynucleotides encoding these polypeptides, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences. 1. An isolated , non-naturally occurring TALE DNA-binding polypeptide comprising a plurality of TALE-repeat units , each TALE-repeat unit comprising a repeat variable di-residue region (RVD) , and wherein the TALE DNA-binding polypeptide comprises at least 3 non-canonical RVDs.2. The TALE DNA-binding polypeptide of claim 1 , wherein the amino acid at position 11 in at least one of the repeat units has been altered as compared to a naturally occurring TALE repeat unit.3. The TALE DNA-binding polypeptide of claim 2 , wherein the amino acid at position 11 is altered to an amino acid selected from the group consisting of an Alanine (A) claim 2 , Cysteine (C) claim 2 , Glycine (G) claim 2 , Histidine (H) claim 2 , Lysine (K) claim 2 , Methionine (M) claim 2 , Asparagine (N) claim 2 , Glutamine (Q) and Arginine (R).4. A fusion protein comprising the TALE DNA-binding polypeptide of and a functional domain5. The fusion protein of claim 4 , wherein the functional domain is selected from the group consisting of a transcriptional activator claim 4 , a transcriptional repressor claim 4 , methyltransferase and a nuclease cleavage domain.6. A polynucleotide encoding the TALE DNA-binding protein of .7. A polynucleotide encoding the fusion protein of .8. An isolated host cell comprising the TALE DNA-binding protein of .9. The isolated host cell of claim 8 , wherein the cell is a eukaryotic cell.10. The isolated host cell of claim 9 , wherein the cell is a mammalian or plant cell.11. The isolated host cell of claim 10 , wherein the mammalian cell is a stem cell.12. The fusion protein according to claim 5 , wherein the nuclease cleavage domain ...

PREPARATION AND USE OF PLANT EMBRYO EXPLANTS FOR TRANSFORMATION

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation. 1. A method for obtaining transformable plant tissue comprising:(a) obtaining a plant seed; and(b) preparing an explant from the plant seed under conditions wherein the explant does not germinate and remains viable and competent for genetic transformation, thereby producing transformable plant tissue.2. The method of claim 1 , wherein the explant comprises a substantially intact seed modified to expose at least a first transformable cell of the seed.3. The method of claim 2 , wherein preparing the explant comprises drilling at least a first aperture in the seed.4. The method of claim 2 , wherein preparing the explant comprises nicking the seed.5. The method of claim 2 , wherein the substantially intact seed comprises a meristem of the seed.6. The method of claim 1 , further comprising the step of(c) transforming at least a first cell of the explant with a selected DNA.7. The method of claim 6 , further comprising the step of:(d) regenerating a transgenic plant from said cell, wherein the plant is stably transformed with the selected DNA.8. The method of claim 7 , further defined as comprising storing the explant prior to step (d) under conditions wherein the explant does not germinate.9. The method of claim 1 , further defined as comprising dehydrating said seed and/or explant prior to or after preparing the explant.10. The method of claim 7 , further defined as comprising increasing the moisture content of the explant prior to or concurrently with step (d).11. The method of claim 1 , wherein preparing the explant is carried out in the absence of a liquid.12. The method of claim 1 , ...

MODULAR DNA-BINDING DOMAINS AND METHODS OF USE

The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell. 1. A method of modulating expression of a target gene in a cell , the method comprising introducing into the cell a non-naturally occurring fusion protein comprising an artificial transcription activator-like (TAL) effector repeat domain of contiguous repeat units 33 to 35 amino acids in length , wherein the repeat domain is engineered for recognition of a predetermined nucleotide sequence , wherein the repeat domain is contained in a polypeptide controlling the transcription of a gene , and wherein the fusion protein recognizes the predetermined nucleotide sequence.2. The method of claim 1 , wherein the cell is a eukaryotic cell.3. The method of claim 1 , wherein the cell is an animal cell.4. The method of claim 1 , wherein the cell is a mammalian cell.5. The method of claim 1 , wherein the cell is a human cell.6. The method of claim 1 , wherein the cell is a plant cell.7. The method of claim 1 , wherein the cell is a prokaryotic cell. This application is a continuation of U.S. application Ser. No. 13/019,526, filed Feb. 2, 2011, which is a continuation-in-part of U.S. application Ser. No. 13/016,297, filed Jan. 28, 2011 which is a continuation of International Application PCT/IB2010/000154, filed Jan. 12, 2010, which designates the U.S and was published by the International Bureau in English on Jul. 15, 2010, and which claims the benefit of U.S. Provisional Patent Application No. 61/225,043, filed Jul. 13, 2009, European (EP) Patent Applicaton No. 09165328.7, filed Jul. 13, 2009, German (DE) Patent Application No. 102009004659.3, ...

Als inhibitor herbicide tolerant beta vulgaris mutants

The present invention relates to an ALS inhibitor herbicide tolerant Beta vulgaris plant and parts thereof comprising a mutation of an endogenous acetolactate synthase (ALS) gene, wherein the ALS gene encodes an ALS polypeptide containing an amino acid different from tryptophan at a position 569 of the ALS polypeptide.

METHODS FOR CONDENSING A HUMID GAS

A method for processing a fluid includes sparging a fluid within the compartment of a container with an initial gas so that the initial gas passes through a portion of the fluid to form a humid gas within the compartment. The humid gas is passed out of the compartment of the container and into a flexible condenser bag. The humid gas within the condenser bag is cooled so as to separate the humid gas within the condenser bag into a condensed fluid and a dehumidified gas. The condensed fluid and the dehumidified gas is then removed from the condenser bag. 1. A method for processing a fluid , the method comprising:sparging a fluid within the compartment of a container with an initial gas so that the initial gas passes through a portion of the fluid to form a humid gas within the compartment;passing the humid gas out of the compartment of the container and into a flexible condenser bag;cooling the humid gas within the condenser bag so as to separate the humid gas within the condenser bag into a condensed fluid and a dehumidified gas; andremoving the condensed fluid and the dehumidified gas from the condenser bag.2. The method as recited in claim 1 , wherein the flexible condenser bag is comprised one or more flexible sheets of polymeric material.3. The method as recited in claim 1 , wherein the container comprises a flexible bag comprised one or more flexible sheets of polymeric material.4. The method as recited in claim 1 , further comprising:dispensing media and cells or microorganisms into the compartment of the container to form the fluid; andmixing the fluid within the container.5. The method as recited in claim 1 , further comprising returning the condensed fluid back into the compartment of a container.6. The method as recited in claim 1 , further comprising exhausting the dehumidified gas through a filter and into the open environment.7. The method as recited in claim 1 , wherein the condenser bag is mounted on a condenser and the step of cooling the humid gas ...

Artificial biological materials produced by self-assembly of photosynthetic unicells

Methods for the self-assembly of photosynthetic unicells into a multicellular shape are provided. DNA constructs as well as methods for integration of the DNA constructs into the genomes of photosynthetic unicells for the expression of cell wall proteins or cell adhesion molecules for the self-assembly of photosynthetic unicells into a multicellular shape are also disclosed.

SYSTEM FOR AND METHOD OF SEPARATING GERM FROM GRAINS USED FOR ALCOHOL PRODUCTION

Methods of and device for separating germs from grains used for alcohol production are provided. The principle of density difference is able to be used to separate germs from a slurry. By adjusting the density and/or viscosity of the slurry, higher germ recovering rate is attended. The method of adjusting the density and/or viscosity includes recycling a cook-water to reduce the viscosity of the slurry before fermentation and pre-concentrating a whole stillage at an evaporator and adding a syrup to the whole stillage after fermentation. An air flotation unit is able to be used to increase oil and germ recovery rate. 1. A method of separating germs from a whole stillage comprising increasing a liquid density of a higher density layer of a slurry after a distilling step.2. The method of claim 1 , wherein the increasing the liquid density comprises making the higher density layer having a solid content in the range of 15%-30% DS.3. The method of claim 1 , wherein the increasing the liquid density comprises optimizing the higher density layer having a solid content in the range of 17%-24% DS.4. The method of claim 1 , wherein the increasing the liquid density comprises sending the whole stillage to an evaporator before separating germs.5. The method of claim 1 , wherein the increasing the liquid density comprises adding a concentrated syrup to the whole stillage.6. The method of claim 1 , wherein the slurry comprises the whole stillage.7. The method of claim 1 , further comprising separating germs from fiber using a germ cyclone claim 1 , a decanter or a combination thereof.8. The method of claim 7 , wherein the germ cyclone claim 7 , the decanter or both comprises a single or a multiple stage separating device.9. The method of claim 7 , wherein the germ cyclone claim 7 , the decanter or both comprises a counter current setup.10. The method of claim 1 , further comprising recovering germs from a thin stillage layer.11. The method of claim 10 , further comprising ...

METHOD OF EXTRACTING EMBRYOS FROM KERNELS OF CORN

A method is provided for extracting embryos from the kernels of an ear of corn. In various embodiments, the method includes receiving an extraction solution in a vessel, receiving at least a portion of an ear of corn in the vessel, the portion of the ear of corn including a plurality of kernels having embryos and intact pericarps, sealing the vessel, subjecting the sealed vessel to a vigorous shaking motion to create an embryo mixture, and collecting the embryo mixture. In some embodiments, embryos are separated from the embryo mixture. 1. A method for extracting embryos from the kernels of an ear of corn , said method comprising:receiving an extraction solution in a vessel;receiving at least a portion of an ear of corn in the vessel, the portion of the ear of corn including a plurality of kernels having embryos and intact pericarps;sealing the vessel;subjecting the sealed vessel to a vigorous shaking motion to create an embryo mixture; andcollecting the embryo mixture.2. The method of claim 1 , further comprising separating embryos from the embryo mixture.3. The method of claim 1 , wherein subjecting the vessel to a vigorous shaking motion comprises shaking the vessel in a mechanical device.4. The method of claim 1 , wherein receiving an extraction solution in a vessel comprises receiving a sterile media in the vessel.5. The method of claim 1 , wherein the extraction solution comprises a media mixture.6. The method of claim 5 , wherein the media mixture comprises a mixture of deionized water claim 5 , MS basal salt mixture claim 5 , myo-inositol claim 5 , nicotinic acid claim 5 , pyridoxine.HCl claim 5 , thiamine.HCl claim 5 , vitamin assay casamino acids claim 5 , sucrose claim 5 , and glucose.7. The method of claim 1 , wherein the extraction solution comprises at least one of coconut water claim 1 , coconut milk claim 1 , calcium chloride claim 1 , and cow's milk.8. The method of claim 2 , wherein separating embryos from the embryo mixture comprises filtering the ...

POLYPHENOL PRODUCTION BY VACCINIUM MYRTILLUS CELL CULTURES

Cell cultures of configured to grow in suspension culture in a liquid medium. The cells are derived from one or more plant parts, such as an edible plant part (e.g., a leaf part or a berry part) or a stem part. The cells are adapted to grow to a high density is a relatively short period of time (e.g., about 7 days). In addition, the cells are adapted to produce high concentrations of polyphenols and/or procyanidins and essentially no anthocyanin. Methods for production of polyphenols and/or procyanidins from cells grown in suspension culture are disclosed. 1. A cell culture , comprising:{'i': 'Vaccinium myrtillus', 'a plurality of friable cells in a suspension cell culture, the cells being derived from one or more ofa hypocotyl, a cotyledon, a leaf section, a stem section, or a root section of a seedling; or [{'i': 'Vaccinium myrtillus', 'wherein the plurality of cells are selected to be capable of obtaining a packed cell volume of at least 55% in 7 days of growth, and'}, {'i': 'Vaccinium myrtillus', 'wherein at least 5% of a dry mass of the plurality of cells is comprised of procyanidins.'}], 'a berry, a stem section including a node or an internode, or a leaf section of a mature plant,'}2. The cell culture of claim 1 , wherein the berry includes a berry skin.3Vaccinium myrtillus. The cell culture of claim 1 , wherein at least 10% claim 1 , 15% claim 1 , or 20% of a dry mass of the plurality of cells is comprised of polyphenols.4Vaccinium myrtillus. The cell culture of claim 1 , wherein at least 7.5% of the dry mass of the plurality of cells is comprised of procyanidins.5Vaccinium myrtillus. The cell culture of claim 1 , wherein at least 10% of the dry mass of the plurality of cells is comprised of procyanidins.6Vaccinium myrtillus. The cell culture of claim 1 , wherein at least 15% of the dry mass of the plurality of cells is comprised of procyanidins.7Vaccinium myrtillus. The cell culture of claim 1 , wherein the dry mass of the plurality of cells comprises less ...

METHODS OF TRANSFERRING PLANT EMBRYOS TO GERMINATION MEDIUM

Methods of transferring a plurality of plant somatic embryos to germination medium are provided. 1. A method of transferring a plurality of plant somatic embryos to germination medium comprising the steps of:(a) depositing a plurality of plant somatic embryos on a surface of a substrate, wherein the substrate has a top surface and a bottom surface;(b) inverting the substrate with the disposed plurality of plant somatic embryos over germination medium contained in a container such that the plurality of plant somatic embryos disposed on the top surface of the substrate are opposite to and facing a surface of the germination medium; and(c) applying a sufficient force to the bottom surface of the substrate such that the plurality of plant somatic embryos are dislodged from the substrate and fall onto the surface of the germination medium.2. The method of claim 1 , wherein the substrate is a porous substrate.3. The method of claim 2 , wherein the porous substrate is a polymeric or nylon membrane mounted in a frame.4. The method of claim 1 , wherein the force is applied by striking the bottom surface of the substrate with one or more implements.5. The method of claim 1 , wherein steps (b) and (c) are performed manually.6. The method of claim 1 , wherein steps (b) and (c) are performed in an automated system.7. The method of claim 6 , wherein step (b) is performed by a robotic arm.8. The method of claim 6 , wherein the force in step (c) is automatically applied by one or more implements striking the bottom surface of the substrate.9. The method of claim 8 , wherein a plurality of implements automatically strike the bottom surface of the substrate simultaneously.10. The method of claim 8 , wherein a plurality of implements automatically strike the bottom surface of the substrate sequentially.11. The method of claim 1 , further comprising placing the container of germination medium and the disposed plurality of plant somatic embryos into a suitable environment for a period ...

COMPOSITION FOR PREVENTING OR TREATING AIDS CONTAINING PLANT STEM CELL LINE DERIVED FROM CAMBIUM OF PANAX GINSENG INCLUDING WILD GINSENG OR GINSENG AS ACTIVE INGREDIENT

The present invention relates to a composition for preventing or treating acquired immunodeficiency syndrome (AIDS), comprising one or more of the following: a homogenous cell line, and a lysate, an extract and a culture thereof as an active ingredient. The homogenous cell line, the lysate, the extract and the culture thereof, which are derived from a natural product, minimize adverse side effects of prior therapeutic agents and safe for the human body. Further, they effectively increase the count of T cells such as CD4+ T cells and decrease the number of HIVs, thereby preventing opportunistic infection, dysneuria, and neoplaia caused by immune incompetence, and ultimately the risk of death. Therefore, they are useful in preventing and treating AIDS, and relieving symptoms of AIDS. 1Panax ginseng. A method of treating acquired immunodeficiency syndrome (AIDS) , the method comprising administering one or more of the following: a cell line , and a lysate , an extract and a culture thereof , wherein the cell line is derived from the cambium of and has the following characteristics:(a) it is in an innately undifferentiated state; and(b) it is a homogeneous cell line.2. The method of claim 1 , wherein the cell line further has the following characteristics:(a) it is present as single cell level during a suspension culture;{'i': 'Panax ginseng', '(b) it has a lower sensitivity to shear stress in a bioreactor, compared to cell lines derived from tissues other than the cambium of ; and'}{'i': 'Panax ginseng', '(c) its growth rate is higher than those of cell lines derived from tissues other than the cambium of and is cultured stably.'}3. The method of claim 1 , wherein the cell line is obtained by using an isolation method comprising the steps of:{'i': 'Panax ginseng', '(a) obtaining a cambium-containing tissue;'}(b) culturing the cambium-containing tissue in a medium supplemented with IAA (indole-3-acetic acid) or IBA (indole-3-butyric acid) to induce a cell line derived ...

COTTON VARIETY 15R535B2XF

The invention relates to the novel cotton variety designated 15R535B2XF. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 15R535B2XF. Also provided by the invention are methods of using cotton variety 15R535B2XF and products derived therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 15R535B2XF with itself or another cotton variety and plants and seeds produced by such methods. 1. A plant of cotton variety 15R535B2XF , wherein a sample of seed of said variety has been deposited under ATCC Accession No. ______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. The plant part of claim 2 , further defined as pollen claim 2 , a meristem claim 2 , a cell claim 2 , or an ovule.4. A seed of cotton variety 15R535B2XF claim 2 , wherein a sample of seed of said variety has been deposited under ATCC Accession No. ______.5. A cotton plant that expresses all of the morphological and physiological characteristics of cotton variety 15R535B2XF claim 2 , wherein a sample of seed of said variety has been deposited under ATCC Accession No. ______.6. A method of producing cotton seed claim 1 , wherein the method comprises crossing the plant of with itself or a second cotton plant.7. The method of claim 6 , wherein the method comprises crossing the plant of cotton variety 15R535B2XF with a second claim 6 , distinct cotton plant to produce an Fhybrid cotton seed.8. A cotton seed produced by the method of .9. A cotton plant produced by growing the seed of .10. A composition comprising a seed of cotton variety 15R535B2XF comprised in plant seed growth media claim 8 , wherein a sample of seed of said variety has been deposited under ATCC Accession No. ______.11. The composition of claim 10 , wherein the growth media is soil or a synthetic cultivation medium.12. A plant of cotton variety 15R535B2XF further ...

COTTON VARIETY 15R537

The invention relates to the novel cotton variety designated 15R537. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 15R537. Also provided by the invention are methods of using cotton variety 15R537 and products derived therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 15R537 with itself or another cotton variety and plants and seeds produced by such methods. 1. A plant of cotton variety 15R537 , wherein a sample of seed of said variety has been deposited under ATCC Accession No. ______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. The plant part of claim 2 , further defined as pollen claim 2 , a meristem claim 2 , a cell claim 2 , or an ovule.4. A seed of cotton variety 15R537 claim 2 , wherein a sample of seed of said variety has been deposited under ATCC Accession No. ______.5. A cotton plant that expresses all of the morphological and physiological characteristics of cotton variety 15R537 claim 2 , wherein a sample of seed of said variety has been deposited under ATCC Accession No. ______.6. A method of producing cotton seed claim 1 , wherein the method comprises crossing the plant of with itself or a second cotton plant.7. The method of claim 6 , wherein the method comprises crossing the plant of cotton variety 15R537 with a second claim 6 , distinct cotton plant to produce an Fhybrid cotton seed.8. A cotton seed produced by the method of .9. A cotton plant produced by growing the seed of .10. A composition comprising a seed of cotton variety 15R537 comprised in plant seed growth media claim 8 , wherein a sample of seed of said variety has been deposited under ATCC Accession No. ______.11. The composition of claim 10 , wherein the growth media is soil or a synthetic cultivation medium.12. A plant of cotton variety 15R537 further comprising a single locus conversion claim 10 , ...

PLANT CELL CULTURE FOR PRODUCTION OF NATURAL PRODUCTS WITH REDUCED FLUCOSINOLATE CONTAMINATION

Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described. 1Brassica. An isolated cabbage cell line , comprising callus cells derived from a plant , the callus cells being selected to grow in suspension cell culture and to produce less than 76.54 μg total glucosinolate per gram fresh cell weight and at least 81.9 mg of anthocyanin per liter of packed cells when grown in cell suspension culture.2. The isolated cabbage cell line of claim 1 , wherein the callus cells are selected to produce anthocyanins at a ratio of anthocyanin to glucosinolate of at least 40:1.3Brassica. The isolated cabbage cell line of claim 1 , wherein the callus cells are selected to have reduced glucosinolate production of at least a 10% decrease as compared to the plant from which the callus cells were derived.4. The isolated cabbage cell line of claim 1 , wherein the callus cells are selected to have reduced glucosinolate production of less than 5 μg of total glucosinolate per gram fresh cell weight.5. The isolated cabbage cell line of claim 1 , wherein the callus cells are selected to produce at least 1119.0 mg ...

FUNGICIDE ENHANCERS EFFECTIVE FOR TREATING PLANTS INFECTED WITH FUNGAL PATHOGENS

The present invention includes compositions and methods of for treating plants infected with fungal pathogens by contacting an infected plant or plant at risk of infection with a fungicidal composition comprising an fungicide selected from copper compound such as copper octanoate or copper hydroxide, or a triazole fungicide such as myclobutanil, propiconazole, tebuconazole or epoxiconazole, an enhancer selected from apyrase inhibitors, e.g., N-(m-toly1)-[1, 1′-biphenyl]-4-sulfonamide, S-heptyl 2-oxo-2H-chromene-3-carbothioate, 3-(N-(4-bromophenyl) sulfamoyl)-N-(3-nitrophenyl) benzamide, or (E)-3-methyl-N-(1-(naphthalen-2-yl) ethylidene) benzohydrazide and, optionally, a phytologically-acceptable inert carrier. 1. A composition comprising an fungicide selected from a copper class or triazole , and an enhancer selected from N-(m-toly1)-[1 , 1′-biphenyl]-4-sulfonamide , S-heptyl 2-oxo-2H-chromene-3-carbothioate , 3-(N-(4-bromophenyl) sulfamoyl)-N-(3-nitrophenyl) benzamide , or (E)-3-methyl-N′ -(1-(naphthalen-2-yl) ethylidene).2. The composition of claim 1 , wherein the fungicide is selected from at least one of copper octanoate claim 1 , myclobutanil claim 1 , propiconazole claim 1 , tebuconazole claim 1 , or epoxiconazole.3. The composition of claim 1 , wherein the composition further comprises a phytologically-acceptable inert carrier.4. The composition of claim 1 , wherein the amount of the fungicide is sub-optimal for treating a fungus.5. The composition of claim 1 , wherein the amount of the fungicide is optimal for treating a fungus and is adapted for application to a fungicide-resistant fungus.6. The composition of claim 1 , wherein the enhancer is provided at 0.005 claim 1 , 0.01 claim 1 , 0.05 claim 1 , 0.1 claim 1 , 0.5 claim 1 , 1.0 claim 1 , 1.1 claim 1 , 1.2 claim 1 , 1.3 claim 1 , 1.4 claim 1 , 1.5 claim 1 , 1.6 claim 1 , 1.7 claim 1 , 1.8 claim 1 , 1.9 claim 1 , 2.0 claim 1 , 2.5 claim 1 , 3.0 claim 1 , 4.0 claim 1 , 5.0 claim 1 , 7.5 claim 1 , and 10% ...

SOYBEAN VARIETY 01064461

The invention relates to the soybean variety designated 01064461. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064461. Also provided by the invention are tissue cultures of the soybean variety 01064461 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064461 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01064461 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124754.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01064461 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124754.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01064461 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01064461 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124754.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN VARIETY 01064571

The invention relates to the soybean variety designated 01064571. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064571. Also provided by the invention are tissue cultures of the soybean variety 01064571 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064571 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01064571 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124755.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01064571 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124755.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01064571 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01064571 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124755.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN VARIETY 01068141

The invention relates to the soybean variety designated 01068141. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01068141. Also provided by the invention are tissue cultures of the soybean variety 01068141 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01068141 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01068141 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124824.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01068141 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124824.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01068141 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01068141 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124824.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN VARIETY 01063576

The invention relates to the soybean variety designated 01063576. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01063576. Also provided by the invention are tissue cultures of the soybean variety 01063576 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01063576 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01063576 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124829.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01063576 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124829.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01063576 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01063576 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124829.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN VARIETY 01064568

The invention relates to the soybean variety designated 01064568. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064568. Also provided by the invention are tissue cultures of the soybean variety 01064568 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064568 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01064568 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124756.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01064568 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124756.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01064568 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01064568 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124756.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

TOMATO HYBRID SVTC0630 AND PARENTS THEREOF

The invention provides seed and plants of tomato line CHI-XF15-9015. The invention thus relates to the plants, seeds, and tissue cultures of tomato line CHI-XF15-9015 and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants. 1. A tomato plant comprising at least a first set of the chromosomes of tomato line CHI-XF15-9015 , a sample of seed of said line having been deposited under ATCC Accession Number PTA-124481.2. A tomato seed that produces the plant of .3. The plant of claim 1 , wherein the plant is a plant of said tomato line CHI-XF15-9015.4. (canceled)5. The seed of claim 2 , wherein the seed is a seed of said tomato line CHI-XF15-9015.6. (canceled)7. A plant part of the plant of claim 1 , wherein the plant part comprises a cell of said plant.8. A tomato plant having all the physiological and morphological characteristics of the plant of .9. A tissue culture of regenerable cells of the plant of .10. A method of vegetatively propagating the tomato plant of claim 1 , the method comprising the steps of:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(a) collecting tissue capable of being propagated from the plant of ; and'}(b) propagating a tomato plant from said tissue.11. A method of introducing a trait into a tomato line claim 1 , the method comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'sub': '1', '(a) utilizing as a recurrent parent the plant of by crossing said plant with a donor tomato plant that comprises a trait to produce Fprogeny;'}{'sub': '1', '(b) selecting an Fprogeny that comprises the trait;'}{'sub': '1', '(c) backcrossing the selected Fprogeny with a plant of the same tomato line used as the recurrent parent in step (a) to produce backcross progeny;'}(d ...

XYLANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

The present invention relates to xylanase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the xylanase variants and methods of using the variants. 1. A xylanase variant , comprising a substitution at one or more positions corresponding to positions 24 , 26 , 36 , 37 , 60 , 71 , 74 , 75 , 76 , 124 , 133 , 155 , 167 , 208 , 317 , and 321 of SEQ ID NO: 1 , wherein the xylanase variant has xylanase activity and wherein the xylanase variant has at least 60% , but less than 100% sequence identity to SEQ ID NO: 1 , 2 , 3 , 4 , 5 and/or 6.2. The xylanase variant of claim 1 , which has at least 70% claim 1 , but less than 100% sequence identity to SEQ ID NO: 1 claim 1 , 2 claim 1 , 3 claim 1 , 4 claim 1 , 5 and/or 6.3. The xylanase variant of claim 1 , wherein the substitution is selected from the group consisting of H24W claim 1 , A26E claim 1 , R36L claim 1 , R36T claim 1 , E37T claim 1 , R60N claim 1 , K71T claim 1 , K71I claim 1 , V74L claim 1 , V74I claim 1 , K75N claim 1 , K75L claim 1 , H76L claim 1 , I155M claim 1 , N167E claim 1 , V208L claim 1 , S317D and G321A.4. The xylanase variant of claim 1 , which is a variant of a parent xylanase wherein the parent xylanase has at least 60% sequence identity to SEQ ID NO: 1.5. (canceled)6. A composition comprising the xylanase variant of and a formulating agent.7. (canceled)8. A granule comprising the xylanase variant of and a formulating agent.9. An animal feed additive comprising the xylanase variant of and one or more components selected from the group consisting of:one or more vitamins;one or more minerals;one or more amino acids;one or more phytogenics;one or more prebiotics;one or more organic acids; andone or more other feed ingredients.10. A liquid formulation comprising the xylanase variant of and optionally 20% to 80% w/w of polyol and/or 0.01% to 2.0% w/w preservative.11. (canceled)12. An animal feed ...

METHODS AND SYSTEMS FOR EXTRACTING DICOT EMBRYOS

Methods and systems are provided which permit extraction of a dicot embryo of a dicot seed without damage to the dicot embryo. Methods disclosed herein provide embryos for uses in plant breeding and research procedures. 1. A method of manually or automatically isolating a dicot embryo from an immature dicot seed having a proximal end and an opposed distal end , wherein , prior to removal of the immature dicot seed from its biological carrier , the proximal end of the immature dicot seed is attached to the biological carrier and the distal end of the immature dicot seed is spaced from the biological carrier , the method comprising:a. providing an isolated immature dicot seed having an opening or hole in the proximal end of the dicot seed, wherein the isolated immature dicot seed is provided following removal of the immature dicot seed from the biological carrier; andb. extracting the dicot embryo through the opening or hole in the proximal end of the isolated immature dicot seed, wherein the dicot embryo is undamaged following extraction.2. The method of claim 1 , wherein claim 1 , following extraction of the dicot embryo claim 1 , the dicot embryo retains an ability to grow with full viability and vigor.3. The method of claim 1 , wherein the step of providing the dicot seed comprises removing at least a portion of the proximal end of the dicot seed without damage to the dicot embryo.4. The method of claim 1 , wherein the immature dicot seed is a sunflower seed.5. The method of claim 1 , wherein the immature dicot seed comprises side portions extending between its proximal and distal ends claim 1 , and wherein the step of extracting the dicot embryo through the hole comprises applying pressure to at least one side portion of the dicot seed to force the dicot embryo through the hole in the proximal end of the dicot seed.6. The method of claim 1 , further comprising forming the opening or hole in the proximal end of the dicot seed.7. The method of claim 1 , wherein ...

Wheat cultivar 07651-10

The invention relates to the wheat cultivar designated 07651-10. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar 07651-10. Also provided by the invention are tissue cultures of the wheat cultivar 07651-10 and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar 07651-10 with itself or another wheat cultivar and plants produced by such methods.

WHEAT CULTIVAR BZ9S09-0300RM

The invention relates to the wheat cultivar designated BZ9S09-0300RM. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar BZ9S09-0300RM. Also provided by the invention are tissue cultures of the wheat cultivar BZ9S09-0300RM and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar BZ9S09-0300RM with itself or another wheat cultivar and plants produced by such methods. 1. A plant of wheat cultivar BZ9S09-0300RM , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. The plant part of claim 2 , further defined as head claim 2 , awn claim 2 , leaf claim 2 , pollen claim 2 , ovule claim 2 , embryo claim 2 , cotyledon claim 2 , hypocotyl claim 2 , meristematic cell claim 2 , root claim 2 , root tip claim 2 , pistil claim 2 , anther claim 2 , floret claim 2 , seed claim 2 , pericarp claim 2 , spike claim 2 , stem claim 2 , and callus.4. A seed that produces the plant of .5. A method of producing wheat seed claim 1 , wherein the method comprises crossing the plant of with itself or a second wheat plant.6. The method of claim 5 , wherein the method is further defined as comprising crossing the plant of wheat cultivar BZ9S09-0300RM with a second claim 5 , distinct wheat plant to produce an Fhybrid wheat seed.7. An Fhybrid wheat seed produced by the method of .8. An Fhybrid wheat plant produced by growing the seed of .9. A composition comprising the seed of comprised in plant seed growth media claim 4 , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.10. The composition of claim 9 , wherein the growth media is soil or a synthetic cultivation medium.11. A plant of wheat cultivar BZ9S09-0300RM claim 9 , further comprising a single locus conversion claim 9 , wherein ...

WHEAT CULTIVAR BZ9S09-0309RM

The invention relates to the wheat cultivar designated BZ9S09-0309RM. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar BZ9S09-0309RM. Also provided by the invention are tissue cultures of the wheat cultivar BZ9S09-0309RM and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar BZ9S09-0309RM with itself or another wheat cultivar and plants produced by such methods. 1. A plant of wheat cultivar BZ9S09-0309RM , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. The plant part of claim 2 , further defined as head claim 2 , awn claim 2 , leaf claim 2 , pollen claim 2 , ovule claim 2 , embryo claim 2 , cotyledon claim 2 , hypocotyl claim 2 , meristematic cell claim 2 , root claim 2 , root tip claim 2 , pistil claim 2 , anther claim 2 , floret claim 2 , seed claim 2 , pericarp claim 2 , spike claim 2 , stem claim 2 , and callus.4. A seed that produces the plant of .5. A method of producing wheat seed claim 1 , wherein the method comprises crossing the plant of with itself or a second wheat plant.6. The method of claim 5 , wherein the method is further defined as comprising crossing the plant of wheat cultivar BZ9S09-0309RM with a second claim 5 , distinct wheat plant to produce an Fhybrid wheat seed.7. An Fhybrid wheat seed produced by the method of .8. An Fhybrid wheat plant produced by growing the seed of .9. A composition comprising the seed of comprised in plant seed growth media claim 4 , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.10. The composition of claim 9 , wherein the growth media is soil or a synthetic cultivation medium.11. A plant of wheat cultivar BZ9S09-0309RM claim 9 , further comprising a single locus conversion claim 9 , wherein ...

WHEAT CULTIVAR F9N12-0151

The invention relates to the wheat cultivar designated F9N12-0151. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar F9N12-0151. Also provided by the invention are tissue cultures of the wheat cultivar F9N12-0151 and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar F9N12-0151 with itself or another wheat cultivar and plants produced by such methods. 1. A plant of wheat cultivar F9N12-0151 , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. The plant part of claim 2 , further defined as head claim 2 , awn claim 2 , leaf claim 2 , pollen claim 2 , ovule claim 2 , embryo claim 2 , cotyledon claim 2 , hypocotyl claim 2 , meristematic cell claim 2 , root claim 2 , root tip claim 2 , pistil claim 2 , anther claim 2 , floret claim 2 , seed claim 2 , pericarp claim 2 , spike claim 2 , stem claim 2 , and callus.4. A seed that produces the plant of .5. A method of producing wheat seed claim 1 , wherein the method comprises crossing the plant of with itself or a second wheat plant.6. The method of claim 5 , wherein the method is further defined as comprising crossing the plant of wheat cultivar F9N12-0151 with a second claim 5 , distinct wheat plant to produce an Fhybrid wheat seed.7. An Fhybrid wheat seed produced by the method of .8. An Fhybrid wheat plant produced by growing the seed of .9. A composition comprising the seed of comprised in plant seed growth media claim 4 , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.10. The composition of claim 9 , wherein the growth media is soil or a synthetic cultivation medium.11. A plant of wheat cultivar F9N12-0151 claim 9 , further comprising a single locus conversion claim 9 , wherein a sample of seed of ...

Wheat cultivar f9n12-0153

The invention relates to the wheat cultivar designated F9N12-0153. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar F9N12-0153. Also provided by the invention are tissue cultures of the wheat cultivar F9N12-0153 and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar F9N12-0153 with itself or another wheat cultivar and plants produced by such methods.

Wheat cultivar fa9s10-0038r

The invention relates to the wheat cultivar designated FA9S10-0038R. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar FA9S10-0038R. Also provided by the invention are tissue cultures of the wheat cultivar FA9S10-0038R and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar FA9S10-0038R with itself or another wheat cultivar and plants produced by such methods.

WHEAT CULTIVAR FA9S10-0049R

The invention relates to the wheat cultivar designated FA9S10-0049R. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar FA9S10-0049R. Also provided by the invention are tissue cultures of the wheat cultivar FA9S10-0049R and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar FA9S10-0049R with itself or another wheat cultivar and plants produced by such methods. 1. A plant of wheat cultivar FA9S 10-0049R , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. The plant part of claim 2 , further defined as head claim 2 , awn claim 2 , leaf claim 2 , pollen claim 2 , ovule claim 2 , embryo claim 2 , cotyledon claim 2 , hypocotyl claim 2 , meristematic cell claim 2 , root claim 2 , root tip claim 2 , pistil claim 2 , anther claim 2 , floret claim 2 , seed claim 2 , pericarp claim 2 , spike claim 2 , stem claim 2 , and callus.4. A seed that produces the plant of .5. A method of producing wheat seed claim 1 , wherein the method comprises crossing the plant of with itself or a second wheat plant.6. The method of claim 5 , wherein the method is further defined as comprising crossing the plant of wheat cultivar FA9S10-0049R with a second claim 5 , distinct wheat plant to produce an Fhybrid wheat seed.7. An Fhybrid wheat seed produced by the method of .8. An Fhybrid wheat plant produced by growing the seed of .9. A composition comprising the seed of comprised in plant seed growth media claim 4 , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.10. The composition of claim 9 , wherein the growth media is soil or a synthetic cultivation medium.11. A plant of wheat cultivar FA9S10-0049R claim 9 , further comprising a single locus conversion claim 9 , wherein a ...

WHEAT CULTIVAR FA9S10-5037W

The invention relates to the wheat cultivar designated FA9S10-5037W. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar FA9S10-5037W. Also provided by the invention are tissue cultures of the wheat cultivar FA9S10-5037W and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar FA9S10-5037W with itself or another wheat cultivar and plants produced by such methods. 1. A plant of wheat cultivar FA9S10-5037W , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. The plant part of claim 2 , further defined as head claim 2 , awn claim 2 , leaf claim 2 , pollen claim 2 , ovule claim 2 , embryo claim 2 , cotyledon claim 2 , hypocotyl claim 2 , meristematic cell claim 2 , root claim 2 , root tip claim 2 , pistil claim 2 , anther claim 2 , floret claim 2 , seed claim 2 , pericarp claim 2 , spike claim 2 , stem claim 2 , and callus.4. A seed that produces the plant of .5. A method of producing wheat seed claim 1 , wherein the method comprises crossing the plant of with itself or a second wheat plant.6. The method of claim 5 , wherein the method is further defined as comprising crossing the plant of wheat cultivar FA9S10-5037W with a second claim 5 , distinct wheat plant to produce an Fhybrid wheat seed.7. An Fhybrid wheat seed produced by the method of .8. An Fhybrid wheat plant produced by growing the seed of .9. A composition comprising the seed of comprised in plant seed growth media claim 4 , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.10. The composition of claim 9 , wherein the growth media is soil or a synthetic cultivation medium.11. A plant of wheat cultivar FA9S10-5037W claim 9 , further comprising a single locus conversion claim 9 , wherein a ...

WHEAT CULTIVAR Y9A12-0004

The invention relates to the wheat cultivar designated Y9A12-0004. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar Y9A12-0004. Also provided by the invention are tissue cultures of the wheat cultivar Y9A12-0004 and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar Y9A12-0004 with itself or another wheat cultivar and plants produced by such methods. 1. A plant of wheat cultivar Y9A12-0004 , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. The plant part of claim 2 , further defined as head claim 2 , awn claim 2 , leaf claim 2 , pollen claim 2 , ovule claim 2 , embryo claim 2 , cotyledon claim 2 , hypocotyl claim 2 , meristematic cell claim 2 , root claim 2 , root tip claim 2 , pistil claim 2 , anther claim 2 , floret claim 2 , seed claim 2 , pericarp claim 2 , spike claim 2 , stem claim 2 , and callus.4. A seed that produces the plant of .5. A method of producing wheat seed claim 1 , wherein the method comprises crossing the plant of with itself or a second wheat plant.6. The method of claim 5 , wherein the method is further defined as comprising crossing the plant of wheat cultivar Y9A12-0004 with a second claim 5 , distinct wheat plant to produce an Fhybrid wheat seed.7. An Fhybrid wheat seed produced by the method of .8. An Fhybrid wheat plant produced by growing the seed of .9. A composition comprising the seed of comprised in plant seed growth media claim 4 , wherein a sample of seed of said cultivar has been deposited under ATCC Accession No. ______.10. The composition of claim 9 , wherein the growth media is soil or a synthetic cultivation medium.11. A plant of wheat cultivar Y9A12-0004 claim 9 , further comprising a single locus conversion claim 9 , wherein a sample of seed of ...

LETTUCE VARIETY 45-175 RZ

The present invention relates to a seed designated 45-175 RZ. The present invention also relates to a sativa plant produced by growing the 45-175 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 45-175 RZ. 1. A seed of lettuce variety 45-175 RZ , a sample of seed of said variety having been deposited under NCIMB accession No. 42609.2. A lettuce plant grown from the seed of .3. The lettuce plant of claim 2 , which is a plant grown from seed having been deposited under NCIMB accession No. 42609.4. A lettuce plant claim 2 , or a part thereof claim 2 , having all the physiological and morphological characteristics of the lettuce plant of .5. A part of the plant of claim 2 , wherein said part comprises a microspore claim 2 , pollen claim 2 , ovary claim 2 , ovule claim 2 , embryo sac claim 2 , egg cell claim 2 , cutting claim 2 , root claim 2 , stem claim 2 , cell or protoplast.6. A tissue culture of regenerable cells or protoplasts from the lettuce plant of .7. The tissue culture as claimed in claim 6 , wherein said cells or protoplasts of the tissue culture are derived from a tissue comprising a leaf claim 6 , pollen claim 6 , embryo claim 6 , cotyledon claim 6 , hypocotyl claim 6 , meristematic cell claim 6 , root claim 6 , root tip claim 6 , anther claim 6 , flower claim 6 , seed or stem.8. A lettuce plant regenerated from the tissue culture of or claim 6 , wherein the regenerated plant expresses all of the physiological and morphological characteristics of lettuce variety 45-175 RZ claim 6 , a sample of seed of said variety having been deposited under NCIMB accession No. 42609.9. A method of vegetatively propagating a plant of lettuce variety 45-175 RZ comprising (a) collecting tissue capable of being propagated from a plant of lettuce 45-175 RZ claim 6 , a sample of seed of said variety having been deposited under NCIMB accession No. 42609 claim 6 , (b) cultivating the tissue to obtain proliferated ...

Modulating Laccase Enzyme to Regulate Cell Wall Biosynthesis and Recalcitrance in Plants

This disclosure provides genetically modified plants having desirable levels of sugar release and syringyl/guaiacyl (S/G) ratio; methods of genetically modifying plants to modulate sugar release and S/G ratio; and uses of such plants. The inventors have determined that genetic modification of a laccase gene (LAC2) from , encoded by locus Potri.008G064000 resulted in transgenic trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. Plants with altered sugar release, and S/G ratio, based on modulation of the expression or activity of the LAC2 gene, have divergent uses including pulp and paper production, and biofuel and bioproducts production. 1. A genetically modified plant characterized by a reduced expression of the LAC2 gene as compared to a control plant.2Populus, Manihot, Gossypium, Eucalyptus, Medicago, Arabidopsis, Solanum, OryzaZea.. The genetically modified plant of claim 1 , wherein said genetically modified plant belongs to a genus selected from the group consisting of and3Populus balsamifera, Populus deltoides, Populus trichocarpa, Populus tremuloides, Populus tremula, Populus albaPopulus maximowiczii.. The genetically modified plant of claim 2 , wherein the plant is selected from the group consisting of and4. The genetically modified plant of claim 1 , wherein the reduced expression of the LAC2 gene is achieved by a method selected from the group consisting of introducing a nucleic acid inhibitor claim 1 , the CRISPR/Cas system claim 1 , the Cre/Lox system claim 1 , the TALEN system claim 1 , and homologous recombination.5. The genetically modified plant of claim 4 , wherein said nucleic acid inhibitor is selected from the group consisting of an antisense RNA claim 4 , a small interfering RNA claim 4 , an RNAi microRNA claim 4 , an artificial microRNA claim 4 , and a ribozyme.6. A method for biofuel production claim 1 , comprising using the plant of in a biofuel fermentation process.7. An expression vector claim 1 , ...

GLYCOSYLATED LYSOSOMAL PROTEINS, METHOD OF PRODUCTION AND USES

The present invention relates to a lysosomal protein composition comprising a plurality of lysosomal proteins that are potentially diversely glycosylated according to a glycosylation pattern, wherein said glycosylation pattern has at least 45% paucimannosidic N-glycans; a method of manufacturing the lysosomal protein composition in a bryophyte plant or cell, and medical and non-medical uses of the lysosomal protein composition. E.g. the lysosomal protein can be a-Galactosidase for the treatment of Fabry Disease or β-Glucoceramidase for the treatment of Gaucher's Disease. The unique glycosylation results in improved therapeutic efficacy—surprisingly even without mannose-6-phosphate that is common for CHO cell produced lysosomal proteins. 1. A method of manufacturing a lysosomal protein composition comprising expressing a transgene encoding a lysosomal protein in a bryophyte plant or cell , wherein said lysosomal protein is expressed with a N-terminal secretory signal , wherein said secretory signal is optionally removed during intracellular processing , and said method further comprises obtaining an expressed lysosomal protein from said plant or cell.2. The method of wherein the expressed lysosomal protein is obtained from secreted matter of the plant or cell claim 1 , preferably without disrupting the producing cells or plant.3. The method of claim 1 , wherein the lysosomal protein lacks a C-terminal vacuolar signal with the sequence VDTM (SEQ ID NO: 1) and/or lacks a C-terminal ER retention signal with the sequence KDEL (SEQ ID NO: 2).4. The method of claim 1 , wherein the lysosomal protein lacks any C-terminal ER retention signal sequence and/or lacks any C-terminal vacuolar signal sequence.5. The method of claim 1 , wherein the lysosomal protein comprises an expressed amino acid sequence that terminates on the C-terminus with the amino acids of a native lysosomal protein or a truncation thereof.6P. patens. The method of claim 1 , wherein the bryophyte plant or ...

FUNGICIDE ENHANCERS EFFECTIVE FOR TREATING PLANTS INFECTED WITH FUNGAL PATHOGENS

The present invention includes compositions and methods of for treating plants infected with fungal pathogens by contacting an infected plant or plant at risk of infection with a fungicidal composition comprising an fungicide selected from copper compound such as copper octanoate or copper hydroxide, or a triazole fungicide such as myclobutanil, propionazoie, tebuconazole or epoxiconazole, an enhancer selected from apyrase inhibitors, e.g., N-(m-toly)-[1,1′-biphenyl]-4-sulfonamide, S-heptyl 2-oxo-2H-chromene 3-carbothioate, 3-(N-(4-bromophenyl) sulfamoyl)-N-(3-nitrophenyl) benzamide, or (E)-3-methyl-N-(1-(naphthalen-2-yl) ethylidene) benzohydrazide and, optionally, a phytologically-acceptable inert carrier. 1. A composition comprising an fungicide selected from a copper class or triazole , and an enhancer selected from N-(m-tolyl)-[1 ,1′-biphenyl]-4-sulfonamide , S-heptyl 2-oxo-2H-chromene-3-carbothioate , 3-(N-(4-bromophenyl) sulfamoyl)-N-(3-nitrophenyl) benzamide , or (E)-3-methyl-N′-(1-(naphthalen-2-yl) ethylidene).2. The composition of claim 1 , wherein the fungicide is selected from at least one of copper octanoate claim 1 , myclobutanil claim 1 , propiconazole claim 1 , tebuconazole claim 1 , or epoxiconazole.3. The composition of claim 1 , wherein the composition further comprises a phytologically-acceptable inert carrier.4. The composition of claim 1 , wherein the amount of the fungicide is sub-optimal for treating a fungus.5. The composition of claim 1 , wherein the amount of the fungicide is optimal for treating a fungus and is adapted for application to a fungicide-resistant fungus.6. The composition or claim 1 , wherein the enhancer is provided at 0.005 claim 1 , 0.01 claim 1 , 0.05 claim 1 , 0.1 claim 1 , 0.5 claim 1 , 1.0 claim 1 , 1.1 claim 1 , 1.2 claim 1 , 1.3 claim 1 , 1.4 claim 1 , 1.5 claim 1 , 1.6 claim 1 , 1.7 claim 1 , 1.8 claim 1 , 1.9 claim 1 , 2.0 claim 1 , 2.5 claim 1 , 3.0 claim 1 , 4.0 claim 1 , 5.0 claim 1 , 7.5 claim 1 , and 10% weight ...

Biomembrane, Closed Structure with Biomembrane Characteristics or Cellular Compartment Derived from Natural Sources and/or Self-Assembly Techniques, Preparation Method and Applications Thereof

The present invention provides a method of preparing biomembrane, closed structure with biomembrane characteristics or cellular compartment, comprising the following steps: 1), acquire biological cells from natural tissues or natural biological species; 2), culture the cells obtained in step 1) massively in an appropriate environment; 3), acquire the lysates of cells in step 2), and extracting the biomembrane, closed structure with biomembrane characteristics and cellular compartment through differential centrifugation, density gradient centrifugation or dual-phase extraction individually or a combination of two methods or a combination of three methods thereof. The membrane is a natural biomembrane, closed structure with biomembrane characteristics and cellular compartment, which can be used for package of active ingredients in various fields. 1. A method of preparing a biomembrane in vitro , comprising the steps of: 1) , acquiring biological cells from natural tissues or natural biological species; 2) , culturing the cells obtained in step 1) massively in an appropriate environment; 3) , acquiring the lysates of the cells in step 2) , then carrying out separation and purification in vitro with different methods to obtain various biomembranes or mixtures; the methods for acquiring biomembranes in vitro herein include differential centrifugation , density gradient centrifugation and dual-phase extraction , individually or a combination of two methods or a combination of three methods thereof , to extract the desired biomembranes.2. The method according to claim 1 , wherein the differential centrifugation extraction method comprises the following steps: centrifuge the cell lysate at the first high-speed to obtain a supernate and then certrifuge the supernate at a second speed less than the first high-speed to get the precipitate as the desired biomembranes.3. The method according to claim 2 , wherein the first high-speed is 1.5 claim 2 , 1 claim 2 , 2 or 3 times of ...

NANOPARTICLE MEDIATED DELIVERY OF SEQUENCE SPECIFIC NUCLEASES

Provided are methods for introducing a sequence-specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. 1. A method of introducing a sequence specific nuclease (SSN) into a plant cell comprising an intact cell wall , the method comprising:providing the plant cell;coating a nanoparticle with a SSN;placing the cell having a cell wall and the coated nanoparticle in contact with each other; andallowing uptake of the nanoparticle and the SSN into the plant cell;wherein the nanoparticle is selected from the group consisting of porous nanoparticles, mesoporous nanoparticles, silica nanoparticles, polymer nanoparticles, tungsten nanoparticles, gelatin nanoparticles, nanoshells, nanocores, nanospheres, nanorods, magnetic nanoparticles, semiconductor nanoparticles, nanomatrices, dendrimeric nanomatrices and combinations thereof.2. The method according to claim 1 , wherein coating a nanoparticle with a SSN comprises immobilizing the SSN via noncovalent absorption on the surface of the nanoparticle.3. The method according to claim 1 , further comprising absorbing the SSN into the nanoparticle.4. The method according to claim 1 , further comprising allowing uptake of the nanoparticle into a compartment of the plant cell.5. The method according to claim 4 , further comprising coating the nanoparticle with a cell penetrating peptide and or subcellular compartment targeting protein.6. The method according to claim 4 , wherein the compartment is selected from the group consisting of cytosol claim 4 , nucleus claim 4 , tonoplasts claim 4 , plastid claim 4 , etioplast claim 4 , chromoplast claim 4 , leucoplast claim 4 , elaioplast claim 4 , proteinoplast claim 4 , amyloplast claim 4 , chloroplast claim 4 , and the lumen of the double membrane.7OryzaArabidopsisRicinus. The method according to claim 1 , wherein the plant cell comprising a ...

Methods for plant transformation using spectinomycin selection

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

SULFONYLUREA-TOLERANT SUNFLOWER PLANTS

This invention relates to sunflower lines and hybrids tolerant to sulfonylurea herbicides wherein the tolerance trait is obtained using induced mutagenesis and artificial selection, and to a method for selectively controlling undesired vegetation, including parasitic weeds, by applying sulfonylurea herbicides to sulfonylurea-tolerant sunflower crops. 1. A method for producing a sunflower line containing a highly heritable trait conferring tolerance to sulfonylurea herbicides , wherein the method comprises:(a) treating sunflower seeds with a mutagenic agent;(b) growing the treated seeds into mature plants to produce second-generation seeds;(c) harvesting the second-generation seeds;(d) germinating the second-generation seeds in the presence of an selectably effective amount of a sulfonylurea herbicide to select for survival only germinated seeds containing a trait conferring tolerance to the sulfonylurea herbicide; and(e) growing a surviving germinated seed into a mature plant to produce through self-pollination seeds of the sunflower line containing the heritable trait.2. A sunflower seed containing a highly heritable trait conferring tolerance to sulfonylurea herbicides , wherein said trait is obtained through mutagenesis.3. A seed of designated sunflower line M7 claim 2 , representative seed of said line M7 having been deposited under ATCC Deposit Nos. PTA-2295 and PTA-2296.4. A seed of designated sunflower line M11 claim 2 , representative seed of said line M11 having been deposited under ATCC Deposit Nos. PTA-2767 and PTA-2768.5. A seed of designated sunflower line M12 claim 2 , representative seed of said line M12 having been deposited under ATCC Deposit Nos. PTA-2769 and PTA-2770.6. A sunflower plant claim 2 , or a part thereof claim 2 , grown from a seed of claim 2 , or .7Orobanche. A seed of further containing a trait conferring resistance to parasitism.8. A sunflower plant claim 2 , or a part thereof claim 2 , produced by growing the seed of .9. Pollen of ...

CUCUMBER HYBRID DRCE1266 AND PARENTS THEREOF

The invention provides seed and plants of cucumber hybrid DRCE1266 and the parent lines thereof. The invention thus relates to the plants, seeds, and tissue cultures of cucumber hybrid DRCE1266 and the parent lines thereof and to methods for producing a cucumber plant produced by crossing such plants with themselves or with another cucumber plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants. 1. A cucumber plant comprising at least a first set of the chromosomes of cucumber line EUR-Y316-1044GY or cucumber line EURY314-1032GY , a sample of seed of said lines having been deposited under ATCC Accession Number PTA-124221 and ATCC Accession Number PTA-124220 , respectively.2. A cucumber seed that produces the plant of .3. The plant of claim 1 , wherein the plant is a plant of said cucumber line EUR-Y316-1044GY or cucumber line EURY314-1032GY.4. The plant of claim 1 , wherein the plant is a plant of cucumber hybrid DRCE1266 claim 1 , a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-124219.5. The seed of claim 2 , wherein the seed is a seed of said cucumber line EUR-Y316-1044GY or cucumber line EURY314-1032GY.6. The seed of claim 2 , wherein the seed is a seed of cucumber hybrid DRCE1266 claim 2 , a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-124219.7. A plant part of the plant of claim 1 , wherein the plant part comprises a cell of said plant.8. A cucumber plant having all the physiological and morphological characteristics of the plant of .9. A tissue culture of regenerable cells of the plant of .10. A method of vegetatively propagating the cucumber plant of claim 1 , the method comprising the steps of:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(a) collecting tissue capable of being propagated from the plant of ; and ...

SOYBEAN VARIETY 01064206

The invention relates to the soybean variety designated 01064206. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064206. Also provided by the invention are tissue cultures of the soybean variety 01064206 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064206 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01064206 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01064206 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-______.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01064206 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01064206 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-______.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

RADIOFREQUENCY DEVICE FOR INCREASING INTRACELLULAR BIOACTIVE SUBSTANCE AND PLANT CELL CULTURE METHOD USING THE SAME

There are provided a radiofrequency device for increasing amount of a bioactive substance in a plant cell and a plant cell culture method for increasing amount of useful intracellular secondary metabolites by using the radiofrequency device. The cell culture method of the present invention makes it possible to increase specific secondary metabolites such as daidzein, equol, and the like in a cell and thus can be used for development into various medicines, agricultural pesticides, spices, pigments, food additives, and cosmetics containing bioactive substances. Further, the cell culture method of the present invention improves conventional cell culture methods limitedly used for specific cells or specific metabolites for increasing amount of intracellular bioactive substances and thus can be widely applied to production of cells and secondary metabolites. 1. A cell culture method for increasing amount of an intracellular bioactive substance , the method comprising:(a) a culturing step of inoculating a plant cell into a medium and culturing the plant cell; and(b) a radiofrequency processing step of processing the cultured cell with a radiofrequency for increasing amount of an intracellular bioactive substance in the plant cell.2. The cell culture method of claim 1 , wherein the radiofrequency is used for process in a range of 0.1 to 15 MHz.3. The cell culture method of claim 2 , wherein the culturing step further includes:(a1) a callus inducing step of culturing the plant cell in an MS(Murashige and Skoog) medium containing IAA (indole acetic acid), BAP (6-benzylaminopurine), sucrose, and gelite for 2 to 5 weeks for inducing a callus from a germinated plant; and(a2) an adventitious root inducing step of culturing the callus in an MS medium containing IBA (indole-3-butyric acid), MES monohydrate(2-(N-morpholino), benzyladenine, sucrose, and gelite for 2 to 5 weeks for inducing an adventitious root from the callus.4. The cell culture method of claim 3 , wherein the ...

Cryopreservation using sucralose

Embodiments of the present invention relate to methods of preparing a cell, tissue, organ or plant for cryopreservatiom wherein the method includes contacting the cell, tissue, organ or plant with a composition including sucrose and/or sucralose.

System and Substances for Cryopreservation of Viable Cells

An improved cryopreservation process and substances can involve a cellular collection () in a cryopreservation fluid () that has been conditioned or treated () to enhance the cryopreservation process by adding () energy () such as in the surface energy of a substance in the cryopreservation fluid () prior to reducing energy for that same cryopreservation media for freezing. This can offer enhanced-post-cryogenic viability of the cryopreserved structures or a more optimum cooling curve () for a specific cell type.

HYBRID MELON VARIETY 34-749 RZ

The present invention relates to a seed designated 34-749 RZ. The present invention also relates to a plant produced by growing the 34-749 RZ seed. The invention further relates to methods for producing the melon cultivar, represented by melon variety 34-749 RZ. 1Cucumis melo. A melon () plant designated 34-749 RZ , representative seed of which having been deposited under NCIMB Accession No. 42760.2. A seed of the plant of .3. A part of the plant of claim 1 , wherein said part of the plant is suitable for sexual reproduction.4. The part of the plant as claimed in claim 3 , wherein said part comprises a microspore claim 3 , pollen claim 3 , an ovary claim 3 , an ovule claim 3 , an embryo sac claim 3 , or an egg cell.5. The part of the plant of claim 1 , wherein said part of the plant is suitable for vegetative reproduction.6. The part as claimed in claim 5 , said part comprises a cutting claim 5 , a root claim 5 , a stem claim 5 , a cell claim 5 , or a protoplast.7. A tissue culture of regenerable cells from the melon plant of .8. The cell or protoplast of derived from a leaf claim 6 , pollen claim 6 , an embryo claim 6 , a cotyledon claim 6 , a hypocotyl claim 6 , a meristematic cell claim 6 , a root claim 6 , a root tip claim 6 , an anther claim 6 , a flower claim 6 , a seed claim 6 , or a stem.9Cucumis meloCucumis melo. A method for producing a progeny plant of a melon () plant of claim 6 , comprising crossing the plant of with itself or with another plant claim 6 , harvesting the resultant seed claim 6 , and growing said seed.10Cucumis melo. A progeny plant of a melon () plant of claim 1 , wherein said progeny plant has genetic material for exhibiting the morphological and physiological characteristics as found in melon variety 34-749 RZ; wherein the genetic information is as contained in a plant claim 1 , representative seed of which having been deposited under NCIMB Accession No. 42760.11Cucumis meloCucumis melo. A progeny plant of a melon () plant of claim 1 , ...

TRIPLOID WATERMELON PLANTS WITH A BUSH GROWTH HABIT

The application relates to the field of plant breeding, in particular watermelon breeding. Provided are bush type, triploid watermelon plants (and seeds from which these plants can be grown) and seedless watermelon fruits produced by these plants. Also provided are bush type pollenizer plants and bush type tetraploid plants and methods for producing triploid hybrids having a bush growth type, as well as methods for producing seedless watermelon fruits of high quality. 1Citrullus lanatus. A plant of the species , wherein said plant is triploid and has a bush growth habit.2. The plant according to comprising three copies of a recessive allele designated bush claim 1 , wherein a representative sample of seed containing said allele has been deposited under accession number NCIMB 41907.3. The plant according to claim 1 , wherein the plant is an F1 hybrid produced from a cross between a diploid inbred male parent line claim 1 , which has a bush growth habit claim 1 , and a tetraploid inbred parent line claim 1 , which has a bush growth habit.4. The plant according to claim 1 , wherein the plant has a longest vine length of not longer than 100 cm.5. The plant according to claim 1 , wherein said plant has not more than 15 internodes on the longest vine.6. The plant according to claim 1 , wherein said plant has an average internode length on the longest vine of 7 cm or less claim 1 , but at least 4.7 cm.7. The plant according to claim 1 , wherein the ratio of longest vine length:number of internodes on the longest vine is 7 or less.8. The method according to claim 1 , wherein said plant has a stem diameter of at least 8 mm.9. The plant according to claim 1 , wherein said plant comprises leaves of at least 10 cm long and at least 15 cm wide.10. The plant according to claim 1 , wherein said plant produces one or more fruits with an average fruit weight of at least 5 kg after pollination with diploid pollen.11. Seed from which a plant according to can be grown.12Citrullus ...

FERMENTATION SYSTEMS

The present disclosure pertains to novel cell cultivation and cell and/or cell-derived product production processes that have advantages over currently existing fermentation strategies. The processes and methods according to the present disclosure may be used for an efficient supply of highly viable and metabolically active eukaryotic cells for transient production platforms, as an alternative production process with advantages over currently applied processes (batch, fed-batch or perfusion strategies) and for generating metabolically highly active biomass for subsequent use for transient expression systems or infection by a virus or pseudovirus or in cell-free systems. 1. A cell-density regulated cell cultivation process for the production of eukaryotic cells and/or an eukaryotic cell-derived product , wherein the cell density during the cell cultivation is regulated by adjusting the volume of the cell culture comprising the cells and a nutrient medium in a cultivation vessel , said process comprises the following steps:a) growing the cells in a cell culture having a variable cell culture volume, wherein the culture volume is not regulated to be constant over the entire cell cultivation process,b) measuring the cell density in the cell culture with a density sensor,c) regulating the cell density in the cell culture by adding an appropriate volume of the nutrient medium to the cell culture to keep the cells in the growth phase,d) harvesting a fraction of the cell culture at a desired cell density, wherein the harvested fraction comprises cells and/or a cell-derived product, and wherein the cell culture volume in the vessel is not kept constant continuously by adding nutrient medium into the vessel after harvesting said fraction and/or wherein the volume of the harvested fraction is not immediately replenished by adding nutrient medium into the vessel;e) repeating one or all of the aforementioned steps in the order set forth to allow a repeated harvest of cell ...

Methods and compositions for herbicide tolerance in plants

The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

Functionalization of plant tissues for human cell expansion

Decellularized plant tissues and the use of these plant tissues as scaffolds are disclosed herein. Particularly, decellularized plant tissues are functionalized such to allow for human cell adhesion, thereby allowing for their use as scaffolds for human cells. These scaffolds can then be used in a number of applications/markets, including as research tools for tissue engineering, regenerative medicine, and basic cellular biology.

Improving plant regeneration

The present invention relates to the field of plant breeding and in particular to the regeneration of plants from cells and other tissues. More particularly, the invention provides methods and means for improving callus and shoot formation and regeneration of plants using hyperphyllin or derivatives thereof.

PRODUCTION OF INGENOL, INGENOL ESTERS AND/OR TIGLIAN-3-ONE DERIVATIVES BY EUPHORBIACEAE PLANT CELL SUSPENSION CULTURES

The present invention relates to a method of producing Ingenol, Ingenol esters and/or Tiglian-3-one derivatives, the method comprising the steps of: (a) culturing plant cells obtained from a plant selected from the family Euphorbiaceae in a nutrient medium in a suspension cell culture, wherein the cells produce Ingenol, one or more Ingenol esters and/or one or more Tiglian-3-one derivatives; and (b) recovering the Ingenol, the one or more Ingenol esters and/or the one or more Tiglian-3-one derivatives produced in (a). The present invention further relates to a plant suspension cell culture, wherein the cells are obtained from a plant selected from the family Euphorbiaceae, and wherein the plant cells produce Ingenol and/or one or more Ingenol ester and/or one or more Tiglian-3-one derivatives. The present invention further relates to a plant cell biomass comprising plant cells obtained from the suspension cell culture of the invention, and comprising Ingenol and/or one or more Ingenol esters and/or one or more Tiglian-3-one derivatives. Also, the present invention relates to cryopreserved cells of the plant suspension cell culture of the invention as well as to a method of producing Ingenol, Ingenol esters and/or Tiglian-3-one derivatives based on these cryopreserved cells. 1. A method of producing Ingenol , Ingenol esters and/or Tiglian-3-one derivatives , the method comprising the steps of:(a) culturing plant cells obtained from a plant selected from the family Euphorbiaceae in a nutrient medium in a suspension cell culture, wherein the cells produce Ingenol, one or more Ingenol esters and/or one or more Tiglian-3-one derivatives; and(b) recovering the Ingenol, the one or more Ingenol esters and/or the one or more Tiglian-3-one derivatives produced in (a).2. The method of claim 1 , further comprising prior to step (a) an additional step of:(a-0) culturing explants of a plant from the family Euphorbiaceae on medium, thereby obtaining friable callus material.3. The ...

Nanobionic engineering of organelles and photosynthetic organisms

In one aspect, a composition can include an organelle, and a nanoparticle having a zeta potential of less than −10 mV or greater than 10 mV contained within the organelle. In a preferred embodiment, the organelle can be a chloroplast and the nanoparticle can be a single-walled carbon nanotube associated with a strongly anionic or strongly cationic polymer.

TOMATO HYBRID EX01420200 AND PARENT LINES THEREOF

The invention provides seed and plants of tomato hybrid EX01420200 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of tomato hybrid EX01420200 and the parent lines thereof, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants. 1. A tomato plant of hybrid EX01420200 , a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-9907.2. A seed of tomato hybrid EX01420200 , a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-9907.36-. (canceled)7. A plant part of the plant of .8. The plant part of claim 7 , further defined as a leaf claim 7 , an ovule claim 7 , pollen claim 7 , a fruit claim 7 , or a cell.9. (canceled)10. A tomato plant claim 1 , or a part thereof claim 1 , having all the physiological and morphological characteristics of the tomato plant of .11. A tissue culture of regenerable cells of the plant of .12. The tissue culture according to claim 11 , comprising cells or protoplasts from a plant part selected from the group consisting of embryos claim 11 , meristems claim 11 , cotyledons claim 11 , pollen claim 11 , leaves claim 11 , anthers claim 11 , roots claim 11 , root tips claim 11 , pistil claim 11 , flower claim 11 , seed and stalks.13. A tomato plant regenerated from the tissue culture of claim 12 , wherein the plant comprises all of the morphological and physiological characteristics of tomato hybrid EX01420200.14. A method of vegetatively propagating the plant of comprising the steps of:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(a) collecting tissue capable of being propagated from the plant according to ;'}(b) cultivating said tissue to obtain ...

HYBRID PEPPERS 'E20B30160', 'E20B30161', and 'E20B30162'

Hybrid peppers designated ‘E20B30160’, ‘E20B30161’, and ‘E20B30162’ are disclosed. The invention relates to the seeds of hybrid peppers ‘E20B30160’, ‘E20B30161’, and ‘E20B30162’, to the plants of hybrid peppers ‘E20B30160’, ‘E20B30161’, and ‘E20B30162’, to methods for producing hybrid plants, and to methods for producing other pepper lines, cultivars or hybrids derived from the hybrid peppers ‘E20B30160’, ‘E20B30161’, and ‘E20B30162’. 1. A hybrid pepper seed selected from the group consisting of:hybrid pepper seed designated as ‘E20B30160’, representative sample of seed having been deposited under NCIMB Accession Number X1;hybrid pepper seed designated as ‘E20B30161’, representative sample of seed having been deposited under NCIMB Accession Number X2; andhybrid pepper seed designated as ‘E20B30162’, representative sample of seed having been deposited under NCIMB Accession Number X3.2. A pepper plant produced by growing the seed of .3. A plant part from the plant of .4. The plant part of claim 3 , wherein said part is a leaf claim 3 , a seed claim 3 , a fruit claim 3 , a cell claim 3 , or a portion thereof.5. The plant part of claim 4 , wherein said part is a fruit.6. A pepper plant having all the physiological and morphological characteristics of the pepper plant of .7. A plant part from the plant of .8. The plant part of claim 6 , wherein said part is a leaf claim 6 , a seed claim 6 , a fruit claim 6 , a cell claim 6 , or a portion thereof.9. The plant part of claim 8 , wherein said part is a fruit.10. An Fhybrid pepper plant claim 8 , wherein:{'sub': '1', 'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'the Fhybrid pepper plant has ‘E20B30160’ as a parent where ‘E20B30160’ is grown from the seed of ;'}{'sub': '1', 'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'the Fhybrid pepper plant has ‘E20B30161’ as a parent where ‘E20B30161’ is grown from the seed of ; or'}{'sub': '1', 'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'the Fhybrid pepper plant has ‘ ...

Modular dna-binding domains and methods of use

The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.

CONTINUOUS CULTURE APPARATUS WITH MOBILE VESSEL, ALLOWING SELECTION OF FITTER CELL VARIANTS AND PRODUCING A CULTURE IN A CONTINUOUS MANNER

A method and device for growing plant, animal or stem cells in a continuous manner. 1. A method for growing cells that are not in suspension , comprising:a) providing flexible tubing containing culture medium, and a surface on which the living cells can grow in said tubing, and a system of clamps, each of the clamps being capable of open and closed positions, the clamps being positioned so as to be able to divide the tubing into:i) an upstream region containing unused culture medium;ii) a downstream region containing spent culture medium; andiii) a growth chamber region for growing said cells on the surface, the growth chamber region being disposed between the upstream and downstream regions; andb) closing selected ones of the clamps on the tubing to define the growth chamber region of the tubing between the upstream and downstream regions of the tubing, and introducing viable cells into the growth chamber region;c) cyclically closing and opening selected ones of the clamps to redefine the growth chamber region of the tubing so that a first portion of the previously defined growth chamber region becomes a portion of the downstream region of the tubing, and a portion of the previously defined upstream region of the tubing becomes a portion of the growth chamber region of the tubing; andd) repeating step c);wherein the growth chamber region is subjected to a pre-determined growth condition, and the repetition of step c) selects for those of the living cells that demonstrate an improved rate of reproduction responsive to said growth condition;wherein the living cells are cells that do not grow in suspension.2. The method of claim 1 , further comprising a step of providing a control system that measures culture density in the growth chamber claim 1 , and controls the step of cyclically closing and opening selected ones of the clamps based on the measured culture density.3. The method according to claim 2 , comprising the further step of withdrawing a sample of living ...

Pepper variety nun 70030 pph

The invention relates to the field of Capsicum spp., in particular to a new Capsicum variety designated NUN 70030 PPH plants, seeds and pepper fruits thereof.

PEPPER VARIETY NUN 70038 PPH

The invention relates to the field of spp., in particular to a new variety designated NUN 70038 PPH plants, seeds and pepper fruits thereof. 1. A plant , plant part or seed of pepper variety NUN 70038 PPH , wherein a representative sample of said seed has been deposited under Accession Number NCIMB ______.2. A plant or part thereof grown from the seed of .3. The plant part of claim 2 , further defined as a leaf claim 2 , pollen claim 2 , an ovule claim 2 , a fruit claim 2 , a scion claim 2 , a rootstock claim 2 , cutting claim 2 , flower or a part of any of these or a cell.4capsicum. A plant claim 2 , or a part thereof which does not significantly differ from the plant of in any of the distinguishing characteristics selected from the group consisting of 1) average number of seeds per fruit; 2) heat of (dried) fruit expressed as Scoville units; 3) maturity (at region of adaptability) claim 2 , expressed as days from transplanting until mature red stage; 4) average plant height; 5) average plant width; 6) average seed cavity diameter; 7) a concentrated fruit set; 8) average placental length; 9) average fruit flesh thickness and 10) third internode length.5. A tissue or cell culture of regenerable cells of the plant of .6. The tissue or cell culture according to claim 5 , comprising cells or protoplasts from a plant part selected from the group consisting of embryos claim 5 , meristems claim 5 , cotyledons claim 5 , hypocotyl claim 5 , pollen claim 5 , leaves claim 5 , anthers claim 5 , roots claim 5 , root tips claim 5 , pistil claim 5 , petiole claim 5 , flower claim 5 , fruit claim 5 , seed claim 5 , stem and stalks.7. A pepper plant regenerated from the tissue or cell culture of claim 5 , wherein the plant has all of the physiological and morphological characteristics of the plant of as listed in Table 1 when determined at the 5% significance level.8. A pepper plant regenerated from the tissue or cell culture of claim 5 , wherein the plant has all of the ...

LETTUCE THAT IS RESISTANT TO THE LETTUCE APHID NASONOVIA RIBISNIGRI BIOTYPE 1

The present invention relates to lettuce plants and heads that are resistant to biotype 1 (also called herein Nr:1) as well as to progeny of the plants and propagation material for producing the plants. The invention further relates to a source of the resistance for use in breeding. 1Lactuca sativaNasonovia ribisnigriLactuca serriola. Lettuce () plant , which has the resistance against biotype 1 (Nr:1) as found in plants grown from seeds of 10G.913571 representative seeds of which were deposited under NCIMB accession number 41775.2Nasonovia risbisnigriLactuca serriola. Lettuce plant as claimed in claim 1 , obtainable by introgressing the resistance to Nr:1 as found in 10G.913571 representative seeds of which were deposited under NCIMB accession number 41775 into a plant that is not resistant.3Nasonovia ribisnigriL. serriola. Lettuce plant as claimed in claim 1 , which is resistant to biotype 1 (Nr:1) claim 1 , and which plant is obtainable by crossing a lettuce plant with a plant grown from seeds of 10G.913571 representative seeds of which were deposited with the NCIMB under NCIMB accession number 41775 and selecting in the F2 progeny of the cross that is obtained after selfing the F1 for plants showing the resistance.4. Seed of a lettuce plant as claimed in .5Nasonovia ribisnigri. Seed as claimed in claim 4 , wherein the plant that can be grown from the seed is resistant to biotype 1 (Nr:1).6Nasonovia ribisnigri. Progeny of a lettuce plant or lettuce seeds as claimed in claim 1 , wherein the progeny is resistant to biotype 1 (Nr:1).7. Propagation material suitable for producing a plant as claimed in .8. Propagation material as claimed in claim 7 , which propagation material is formed by seeds.9. Propagation material as claimed in claim 7 , which propagation material is formed by parts of the plant that are suitable for sexual reproduction claim 7 , in particular microspores claim 7 , pollen claim 7 , ovaries claim 7 , ovules claim 7 , embryo sacs and egg cells.10. ...

WHEAT VARIETY W070540B1

A wheat variety designated W070540B1, the plants and seeds of wheat variety W070540B1, methods for producing a wheat plant produced by crossing the variety W070540B1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety W070540B1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety W070540B1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety W070540B1 and to wheat varieties or breeding lines produced by those methods are also provided. 1. A plant , plant part , seed , or plant cell of wheat variety W070540B1 , representative seed of said variety having been deposited under ATCC accession number PTA-123452.2. A wheat seed produced from (i) selfing the plant or plant part of or (ii) crossing of the plant or plant part of with a different wheat plant or plant part.3. A wheat plant or plant part produced by growing the wheat seed of .4. The wheat seed of claim 2 , wherein the seed is an F1 hybrid wheat seed.5. A method for producing a progeny seed claim 3 , the method comprising crossing the wheat plant of claim 3 , to a plant of wheat variety W070540B1 claim 3 , representative seed of said variety having been deposited under ATCC accession number PTA-123452 and producing a progeny seed.6. The method of claim 5 , wherein the method further comprises crossing a plant grown from the progeny seed to a plant of wheat variety W070540B1 and producing a backcrossed seed.7. The backcrossed seed produced by the method of .8. A method for producing a second wheat plant claim 3 , the method comprising applying plant breeding techniques to the wheat plant or plant part of claim 3 , wherein application of said techniques results in the production of a second wheat plant.9. A method for producing a second wheat plant claim 3 , the method comprising doubling haploid seed generated from a cross of the wheat plant or plant part ...

Cotton Variety Sicot 714B3F

The present invention provides a plant of the cotton () variety Sicot 714B3F, or a part, cell, tissue or organ thereof. 1Gossypium hirsutum. A plant of the cotton () variety Sicot 714B3F , or a part , cell , tissue or organ thereof.2Gossypium hirsutum. Seed of the cotton () variety Sicot 714B3F.3. A cotton plant produced by growing the seed of claim 2 , or a part claim 2 , cell claim 2 , tissue or organ of said plant.4. A tissue culture of regenerable cells of the cotton plant of .5. The tissue culture of wherein the tissue culture regenerates plants capable of expressing all the morphological and physiological characteristics of said cotton plant.6. The tissue culture of wherein said tissue culture is generated from cells or protoplasts of a tissue selected from the group consisting of seeds claim 5 , leaves claim 5 , stems claim 5 , pollen claim 5 , anthers claim 5 , ovules claim 5 , embryos claim 5 , cotyledon or hypocotyl.7. A cotton plant regenerated from the tissue culture of claim 4 , or a part claim 4 , cell claim 4 , tissue or organ of said cotton plant claim 4 , wherein said cotton plant has all the morphological and physiological characteristics of Sicot 714B3F.8. A method for producing F1 hybrid cotton seed claim 1 , comprising crossing the cotton plant of with a different cotton plant claim 1 , and harvesting the resultant F1 hybrid cotton seed.9. The method of claim 8 , which comprises obtaining a cotton plant from the F1 hybrid cotton seed claim 8 , and optionally obtaining progeny plants or seed of a second or subsequent generation.10. Hybrid cotton seed produced by the method of .11. A hybrid cotton plant produced by growing the hybrid cotton seed of claim 10 , or a part claim 10 , cell claim 10 , tissue or organ of said hybrid cotton plant.12. A method of producing cotton seed claim 11 , comprising growing the hybrid cotton plant of and harvesting the resultant seed.13. A method of producing a transgenic cotton plant claim 1 , comprising ...

Hybrid Tomato Variety H1648

Hybrid tomato variety ‘H1648’ is described. The tomato variety is a ground-culture hybrid tomato variety suitable for machine harvest, and is adaptable to the climactic conditions of regions such as California (USA), Ontario (Canada), Australia, Portugal, Spain, and Italy. 1. Tomato seed designated as ‘H1648’ , representative sample of seed having been deposited under ATCC Accession Number PTA-124671.2. A plant produced by growing the seed of .3. A plant part from the plant of claim 2 , wherein the plant part comprises at least one cell from tomato variety ‘H1648’.4. The plant part of claim 3 , wherein the part is selected from the group consisting of leaf claim 3 , ovule claim 3 , pollen claim 3 , cell claim 3 , rootstock claim 3 , scion claim 3 , fruit claim 3 , cotyledon claim 3 , meristem claim 3 , anther claim 3 , root claim 3 , root tip claim 3 , pistil claim 3 , flower claim 3 , stem claim 3 , calli claim 3 , stalk claim 3 , hypocotyl claim 3 , pericarp claim 3 , and portion thereof.5. A tomato plant having all the physiological and morphological characteristics of the tomato plant of .6. A plant part from the plant of claim 5 , wherein the plant part comprises at least one cell from tomato variety ‘H1648’.7. The plant part of claim 6 , wherein the part is selected from the group consisting of leaf claim 6 , ovule claim 6 , pollen claim 6 , cell claim 6 , rootstock claim 6 , scion claim 6 , fruit claim 6 , cotyledon claim 6 , meristem claim 6 , anther claim 6 , root claim 6 , root tip claim 6 , pistil claim 6 , flower claim 6 , stem claim 6 , calli claim 6 , stalk claim 6 , hypocotyl claim 6 , pericarp claim 6 , and portion thereof.8. A tomato plant having all the physiological and morphological characteristics of tomato variety ‘H1648’ claim 6 , wherein a representative sample of seed has been deposited under ATCC Accession Number PTA-124671.9. A plant part from the plant of claim 8 , wherein the plant part comprises at least one cell from tomato variety ‘ ...

Hybrid Tomato Variety H1651

Hybrid tomato variety ‘H1651’ is described. The tomato variety is a ground-culture hybrid tomato variety suitable for machine harvest, and is adaptable to the climactic conditions of regions such as California (USA), Ontario (Canada), Australia, Portugal, Spain, and Italy. 1. Tomato seed designated as ‘H1651’ , representative sample of seed having been deposited under ATCC Accession Number PTA-124672.2. A plant produced by growing the seed of .3. A plant part from the plant of claim 2 , wherein the plant part comprises at least one cell from tomato variety ‘H1651’.4. The plant part of claim 3 , wherein the part is selected from the group consisting of leaf claim 3 , ovule claim 3 , pollen claim 3 , cell claim 3 , rootstock claim 3 , scion claim 3 , fruit claim 3 , cotyledon claim 3 , meristem claim 3 , anther claim 3 , root claim 3 , root tip claim 3 , pistil claim 3 , flower claim 3 , stem claim 3 , calli claim 3 , stalk claim 3 , hypocotyl claim 3 , pericarp claim 3 , and portion thereof.5. A tomato plant having all the physiological and morphological characteristics of the tomato plant of .6. A plant part from the plant of claim 5 , wherein the plant part comprises at least one cell from tomato variety ‘H1651’.7. The plant part of claim 6 , wherein the part is selected from the group consisting of leaf claim 6 , ovule claim 6 , pollen claim 6 , cell claim 6 , rootstock claim 6 , scion claim 6 , fruit claim 6 , cotyledon claim 6 , meristem claim 6 , anther claim 6 , root claim 6 , root tip claim 6 , pistil claim 6 , flower claim 6 , stem claim 6 , calli claim 6 , stalk claim 6 , hypocotyl claim 6 , pericarp claim 6 , and portion thereof.8. A tomato plant having all the physiological and morphological characteristics of tomato variety ‘H1651’ claim 6 , wherein a representative sample of seed has been deposited under ATCC Accession Number PTA-124672.9. A plant part from the plant of claim 8 , wherein the plant part comprises at least one cell from tomato variety ‘ ...

Hybrid Tomato Variety H1662

Hybrid tomato variety ‘H1662’ is described. The tomato variety is a ground-culture hybrid tomato variety suitable for machine harvest, and is adaptable to the climactic conditions of regions such as California (USA), Ontario (Canada), Australia, Portugal, Spain, and Italy.

APPARATUS FOR AND METHOD OF PREPARING PLANT TISSUE FOR PLANT PRODUCTION

An apparatus for preparing multiple plant embryos for plant production includes a first station having a first rack system configured to support at least one incubation vessel, a second station having an automated member configured to manipulate the at least one incubation vessel and a third station having a second rack system configured to support the at least one incubation vessel after being manipulated by the automated member. The second station can be selectively adjusted to perform more than one operation required in the development of plant embryos. 122-. (canceled)23. A method of preparing plant tissue for plant production comprising:transporting a first culture vessel from a first station to a second station;manipulating the first culture vessel between a closed position and an open position;supplying at least one substance to the first culture vessel while at the second station; andcontrolling with a controller the steps of transporting, manipulating and supplying.24. The method of claim 23 , wherein the step of supplying at least one substance includes supplying a base media.25. The method of claim 24 , further comprising the step of reconfiguring the second station and supplying a different substance to the first culture vessel claim 24 , the different substance being at least one of a somatic embryo claim 24 , embryogenic tissue claim 24 , organogenic tissue claim 24 , vegetative tissue and seed.26. The method of claim 23 , further comprising the step of inspecting the development of the plant tissue in the first culture vessel.27. The method of claim 26 , wherein the step of inspecting the development of the plant tissue in the first culture vessel comprises using at least one camera coupled to the controller to inspect the development of the plant tissue in the first culture vessel while the first culture vessel is at the second station.28. A method of preparing plant tissue for plant production comprising:transporting a first culture vessel from a ...

Plant Cell Differentiation Promoter

The present invention addresses the problem of providing a plant cell differentiation promoter with which it is possible to promote differentiation from a callus to a normal adventitious embryo, or promote differentiation of an adventitious root or adventitious bud from a plant cutting, and as a result, obtain a regenerated plant with stability. The present invention provides a plant differentiation promoter comprising as the active ingredient a specific ketole fatty acid or derivative thereof. 1. A plant cell differentiation promoting agent , comprising a ketol fatty acid having 4 to 24 carbon atoms , wherein a carbon atom that composes a carbonyl group and a carbon atom that is bound to a hydroxyl group are in the α position.2. The plant cell differentiation promoting agent according to claim 1 , wherein carbon-carbon double bonds are present at 1 to 6 locations in the ketol fatty acid (provided that the number of double bonds does not exceed the number of carbon bonds of the ketol fatty acid).3. The plant cell differentiation promoting agent according to or claim 1 , wherein the number of carbon atoms of the ketol fatty acid is 18 claim 1 , and carbon-carbon double bonds are present at 2 locations.4. The plant cell differentiation promoting agent according to any one of to claim 1 , wherein the ketol fatty acid is 9-hydroxy-10-oxo-12(Z) claim 1 ,15(Z)-octadecadienoic acid.5. The plant cell differentiation promoting agent according to any one of to claim 1 , wherein the plant cell differentiation promoting agent promotes differentiation from a plant callus to an adventitious embryo claim 1 , an adventitious root or an adventitious bud.6. The plant cell differentiation promoting agent according to any one of to claim 1 , wherein the plant cell differentiation promoting agent promotes differentiation from a plant section to an adventitious bud or an adventitious root.7. A method for producing a regenerated plant from a callus claim 1 , comprising a step for applying ...

SOYBEAN VARIETY 01068122

The invention relates to the soybean variety designated 01068122. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01068122. Also provided by the invention are tissue cultures of the soybean variety 01068122 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01068122 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01068122 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124890.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01068122 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124890.4. A method of producing a soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01068122 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01068122 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124890.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN VARIETY 01064594

The invention relates to the soybean variety designated 01064594. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064594. Also provided by the invention are tissue cultures of the soybean variety 01064594 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064594 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01064594 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124891.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01064594 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124891.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01064594 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01064594 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124891.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN VARIETY 01064460

The invention relates to the soybean variety designated 01064460. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064460. Also provided by the invention are tissue cultures of the soybean variety 01064460 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064460 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01064460 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01064460 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-______.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01064460 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01064460 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-______.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN VARIETY 01063959

The invention relates to the soybean variety designated 01063959. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01063959. Also provided by the invention are tissue cultures of the soybean variety 01063959 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01063959 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01063959 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-11124898.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01063959 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-11124898.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01063959 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01063959 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-11124898.11. The plant of claim 10 , wherein the single locus conversion comprises ...

SOYBEAN VARIETY 01064013

The invention relates to the soybean variety designated 01064013. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064013. Also provided by the invention are tissue cultures of the soybean variety 01064013 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064013 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01064013 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124892.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01064013 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124892.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01064013 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01064013 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124892.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN VARIETY 01068143

The invention relates to the soybean variety designated 01068143. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01068143. Also provided by the invention are tissue cultures of the soybean variety 01068143 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01068143 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01068143 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-______.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01068143 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-______.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01068143 with a second claim 4 , nonisogenic soybean plant to produce said soybean seed.6. An Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01068143 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-______.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN VARIETY 01064056

The invention relates to the soybean variety designated 01064056. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01064056. Also provided by the invention are tissue cultures of the soybean variety 01064056 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01064056 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety 01064056 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124897.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. A seed of soybean variety 01064056 claim 1 , wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124897.4. A method of producing soybean seed claim 1 , the method comprising crossing the plant of with itself or a second soybean plant to produce said soybean seed.5. The method of claim 4 , the method further comprising crossing the plant of soybean variety 01064056 with a second claim 4 , nonisogenic soybean plant to produce an Fsoybean seed.6. The Fsoybean seed produced by the method of .7. A soybean plant produced by growing the Fsoybean seed of .8. A composition comprising the seed of comprised in plant seed growth media.9. The composition of claim 8 , wherein the plant seed growth media is soil or a synthetic cultivation medium.10. A plant of soybean variety 01064056 further comprising a single locus conversion claim 8 , wherein said plant otherwise comprises all of the morphological and physiological characteristics of said soybean variety when grown under the same environmental conditions claim 8 , and wherein representative seed of said soybean variety have been deposited under ATCC Accession No. PTA-124897.11. The plant of claim 10 , wherein the single locus conversion comprises a ...

SOYBEAN CULTIVAR S170008

A soybean cultivar designated S170008 is disclosed. The invention relates to the seeds of soybean cultivar S170008, to the plants of soybean cultivar S170008, to the plant parts of soybean cultivar S170008, and to methods for producing progeny of soybean cultivar S170008. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170008. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170008, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170008 with another soybean cultivar. 1. A plant of soybean cultivar S170008 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124445.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170008 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170008 having been deposited under ATCC Accession No. PTA-124445.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170003

A soybean cultivar designated S170003 is disclosed. The invention relates to the seeds of soybean cultivar S170003, to the plants of soybean cultivar S170003, to the plant parts of soybean cultivar S170003, and to methods for producing progeny of soybean cultivar S170003. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170003. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170003, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170003 with another soybean cultivar. 1. A plant of soybean cultivar S170003 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124446.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170003 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170003 having been deposited under ATCC Accession No. PTA-124446.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170002

A soybean cultivar designated S170002 is disclosed. The invention relates to the seeds of soybean cultivar S170002, to the plants of soybean cultivar S170002, to the plant parts of soybean cultivar S170002, and to methods for producing progeny of soybean cultivar S170002. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170002. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170002, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170002 with another soybean cultivar. 1. A plant of soybean cultivar S170002 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124456.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170002 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170002 having been deposited under ATCC Accession No. PTA-124456.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170024

A soybean cultivar designated S170024 is disclosed. The invention relates to the seeds of soybean cultivar S170024, to the plants of soybean cultivar S170024, to the plant parts of soybean cultivar S170024, and to methods for producing progeny of soybean cultivar S170024. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170024. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170024, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170024 with another soybean cultivar. 1. A plant of soybean cultivar S170024 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-11124464.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170024 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170024 having been deposited under ATCC Accession No. PTA-11124464.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from ...

SOYBEAN CULTIVAR S170019

A soybean cultivar designated S170019 is disclosed. The invention relates to the seeds of soybean cultivar S170019, to the plants of soybean cultivar S170019, to the plant parts of soybean cultivar S170019, and to methods for producing progeny of soybean cultivar S170019. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170019. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170019, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170019 with another soybean cultivar. 1. A plant of soybean cultivar S170019 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-1124463.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170019 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170019 having been deposited under ATCC Accession No. PTA-124463.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170020

A soybean cultivar designated S170020 is disclosed. The invention relates to the seeds of soybean cultivar S170020, to the plants of soybean cultivar S170020, to the plant parts of soybean cultivar S170020, and to methods for producing progeny of soybean cultivar S170020. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170020. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170020, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170020 with another soybean cultivar. 1. A plant of soybean cultivar S170020 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124462.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170020 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170020 having been deposited under ATCC Accession No. PTA-124462.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170011

A soybean cultivar designated S170011 is disclosed. The invention relates to the seeds of soybean cultivar S170011, to the plants of soybean cultivar S170011, to the plant parts of soybean cultivar S170011, and to methods for producing progeny of soybean cultivar S170011. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170011. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170011, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170011 with another soybean cultivar. 1. A plant of soybean cultivar S170011 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124461.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170011 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170011 having been deposited under ATCC Accession No. PTA-124461.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170006

A soybean cultivar designated S170006 is disclosed. The invention relates to the seeds of soybean cultivar S170006, to the plants of soybean cultivar S170006, to the plant parts of soybean cultivar S170006, and to methods for producing progeny of soybean cultivar S170006. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170006. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170006, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170006 with another soybean cultivar. 1. A plant of soybean cultivar S170006 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124454.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170006 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170006 having been deposited under ATCC Accession No. PTA-124454.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170014

A soybean cultivar designated S170014 is disclosed. The invention relates to the seeds of soybean cultivar S170014, to the plants of soybean cultivar S170014, to the plant parts of soybean cultivar S170014, and to methods for producing progeny of soybean cultivar S170014. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170014. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170014, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170014 with another soybean cultivar. 1. A plant of soybean cultivar S170014 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124458.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170014 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170014 having been deposited under ATCC Accession No. PTA-124458.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170013

A soybean cultivar designated S170013 is disclosed. The invention relates to the seeds of soybean cultivar S170013, to the plants of soybean cultivar S170013, to the plant parts of soybean cultivar S170013, and to methods for producing progeny of soybean cultivar S170013. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170013. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170013, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170013 with another soybean cultivar. 1. A plant of soybean cultivar S170013 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124457.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170013 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170013 having been deposited under ATCC Accession No. PTA-124457.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170001

A soybean cultivar designated S170001 is disclosed. The invention relates to the seeds of soybean cultivar S170001, to the plants of soybean cultivar S170001, to the plant parts of soybean cultivar S170001, and to methods for producing progeny of soybean cultivar S170001. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170001. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170001, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170001 with another soybean cultivar. 1. A plant of soybean cultivar S170001 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124453.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170001 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170001 having been deposited under ATCC Accession No. PTA-124453.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170022

A soybean cultivar designated S170022 is disclosed. The invention relates to the seeds of soybean cultivar S170022, to the plants of soybean cultivar S170022, to the plant parts of soybean cultivar S170022, and to methods for producing progeny of soybean cultivar S170022. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170022. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170022, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170022 with another soybean cultivar. 1. A plant of soybean cultivar S170022 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124448.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170022 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170022 having been deposited under ATCC Accession No. PTA-124448.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

SOYBEAN CULTIVAR S170017

A soybean cultivar designated S170017 is disclosed. The invention relates to the seeds of soybean cultivar S170017, to the plants of soybean cultivar S170017, to the plant parts of soybean cultivar S170017, and to methods for producing progeny of soybean cultivar S170017. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S170017. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S170017, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S170017 with another soybean cultivar. 1. A plant of soybean cultivar S170017 , representative seed of said soybean cultivar having been deposited under ATCC Accession No. PTA-124447.2. A seed that produces the plant of .3. A cell of the plant of .4. A tissue culture of regenerable cells comprising the cell of .5. A method of soybean breeding claim 1 , said method comprising crossing the plant of with itself or a second soybean plant to produce soybean seed.6. The method of claim 5 , further defined as comprising crossing a plant of soybean cultivar S170017 with a second soybean plant of a different genotype to produce hybrid soybean seed claim 5 , representative seed of said soybean cultivar S170017 having been deposited under ATCC Accession No. PTA-124447.7. A soybean seed produced by the method of .8. A method of producing a plant comprising an added desired trait claim 1 , said method comprising introducing a transgene conferring the desired trait into the plant of .9. The method of claim 8 , wherein the desired trait is selected from the ...

METHOD OF PRODUCTION OF PHYTOCANNABINOIDS FOR USE IN MEDICAL TREATMENTS

A method of producing cannabinoids for use in medical treatments by growing cultured plant cells through tissue culture, the method of comprising the steps of: selecting leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimized depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids. 1Cannabis. A method of producing a cannabinoid from plant cells , the method comprising the steps of:{'i': 'Cannabis', 'selecting a tissue for culture;'}{'i': 'Cannabis', 'growing a cell suspension culture from the selected tissue in a liquid based medium whilst controlling the light exposure of the cell suspension culture to control the content of the cannabinoid produced by the cell suspension culture, the light exposure comprising UV light; and'}collecting and drying cells from the cell suspension culture after growing the cell suspension culture.2. A method according to claim 1 , wherein the drying is freeze-drying.3. A method of further comprising dissolving the dried cells in water.4. A method of further comprising using the dried cells as a medicament or as part of a medicament.5. A method according to claim 1 , wherein the light exposure is controlled such that tissue culture is constantly exposed to PAR during growth of the tissue culture.6. A method according to claim 5 , wherein the PAR is controlled to provide at least 0.2 moles of photons per day.7. A method according to claim 5 , wherein the ...

SOYBEAN VARIETY XB04F13

The invention relates to the soybean variety designated XB04F13. Provided by the invention are the seeds, plants and derivatives of the soybean variety XB04F13. Also provided by the invention are tissue cultures of the soybean variety XB04F13 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety XB04F13 with itself or another soybean variety and plants produced by such methods. 1. A plant of soybean variety XB04F13 , wherein a sample of seed of said variety has been deposited under ATCC Accession No. PTA-121423.2. A plant part of the plant of claim 1 , wherein the plant part comprises at least one cell of said plant.3. The plant part of claim 2 , further defined as pollen claim 2 , a meristem claim 2 , a cell claim 2 , or an ovule.4. A seed of soybean variety XB04F13 claim 2 , wherein a sample of seed of said variety has been deposited under ATCC Accession No. PTA-121423.5. A soybean plant that expresses all of the physiological and morphological characteristics of the plant of .6. A method of producing soybean seed claim 1 , wherein the method comprises crossing the plant of with itself or a second soybean plant.7. The method of claim 6 , wherein the method comprises crossing the plant of soybean variety XB04F13 with a second claim 6 , distinct soybean plant to produce an Fhybrid soybean seed.8. An Fhybrid soybean seed produced by the method of .9. An Fhybrid soybean plant produced by growing the seed of .10. A composition comprising the seed of comprised in plant seed growth media.11. The composition of claim 10 , wherein the growth media is soil or a synthetic cultivation medium.12. A plant produced by introducing a single locus conversion into soybean variety XB04F13 claim 10 , or a selfed progeny thereof comprising the single locus conversion claim 10 , wherein the single locus conversion was introduced into soybean variety XB04F13 by backcrossing or genetic ...

TOMATO VARIETY NUN 09015 TOF

The invention provides a new and distinct hybrid variety of tomato, NUN 09015 TOF. 1. A plant , plant part or seed of tomato variety NUN 09015 TOF , wherein a representative sample of said seed has been deposited under Accession Number NCIMB ______.2. A plant or part thereof grown from the seed of .3. The plant part of claim 2 , further defined as a leaf claim 2 , pollen claim 2 , an ovule claim 2 , a fruit claim 2 , a scion claim 2 , a rootstock claim 2 , cutting claim 2 , flower or a part of any of these or a cell.4Solanum. A plant claim 2 , or a part thereof which does not significantly differ from the plant of in any of the distinguishing characteristics selected from the group consisting of 1) average length of mature fruit (along stem axis); 2 average diameter of mature fruit (at widest point); 3) average weight of mature fruit; 4) average number of locules; 5) an average number of flowers in inflorescence; 6) average number of nodes between early inflorescence; 7) typical fruit shape of type 7; 8) an average length of dedicel (from joint to calyx attachment); 9) pubescence on younger stems and 10) shape of blossom end.5. A tissue or cell culture of regenerable cells of the plant of .6. The tissue or cell culture according to claim 5 , comprising cells or protoplasts from a plant part selected from the group consisting of embryos claim 5 , meristems claim 5 , cotyledons claim 5 , hypocotyl claim 5 , pollen claim 5 , leaves claim 5 , anthers claim 5 , roots claim 5 , root tips claim 5 , pistil claim 5 , petiole claim 5 , flower claim 5 , fruit claim 5 , seed claim 5 , stem and stalks.7. A tomato plant regenerated from the tissue or cell culture of claim 5 , wherein the plant has all of the physiological and morphological characteristics of the plant of as listed in Table 1 when determined at the 5% significance level.8. A tomato plant regenerated from the tissue or cell culture of claim 5 , wherein the plant has all of the physiological and morphological ...