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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 56576. Отображено 100.

12-01-2012 дата публикации

Recombinant polypeptide having pancreatic lipase activity and nucleic acid coding sequence thereof, and their production and use

Номер: US20120009324A1

Автор: Fang-Chueh Liu

Принадлежит: Livestock Res Inst Council of Agriculture

Disclosed herein is an isolated nucleic acid sequence encoding a recombinant polypeptide that has pancreatic lipase activity. Also disclosed herein are a recombinant vector and a recombinant host cell for producing the aforesaid recombinant polypeptide. The aforesaid recombinant polypeptide is adapted for preparation of an animal feed that is able to facilitate utilization of fats therein regarding pigs (especially postweaning pigs) and to enhance growth performance of the pigs.

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Номер записи: 1

19-01-2012 дата публикации

Methods and compositions for cns delivery of heparan n-sulfatase

Номер: US20120014936A1

Автор: Brian Vernaglia, Farah Natoli, Gaozhong Zhu, Jamie Tsung, Jennifer Terew, Jing Pan, Pericles Calias, Richard Pfeifer, YUAN Jiang, Zahra Shahrokh

Принадлежит: Shire Human Genetics Therapies Inc

The present invention provides, among other things, compositions and methods for CNS delivery of lysosomal enzymes for effective treatment of lysosomal storage diseases. In some embodiments, the present invention includes a stable formulation for direct CNS intrathecal administration comprising a heparan N-sulfatase (HNS) protein, salt, and a polysorbate surfactant for the treatment of Sanfilippo Syndrome Type A.

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Номер записи: 2

02-02-2012 дата публикации

Process for production of an enzyme product

Номер: US20120028332A1

Автор: Mette Zacho GRØNFELDT

Принадлежит: Danisco AS

The invention relates to a process for production of an enzyme product having a plurality of enzyme activities obtained by fermentation of an Aspergillus strain.

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Номер записи: 3

16-02-2012 дата публикации

Processing cellulosic biomass

Номер: US20120040408A1

Автор: David Lee, Michael Blaylock, Michael E. Himmell, Michael J. Selig, Roman Brunecky, Stephen R. Decker, Todd Vinzant

Принадлежит: Alliance for Sustainable Energy LLC, Edenspace Systems Corp

Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol are disclosed herein, along with plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols than are typically employed are also disclosed.

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Номер записи: 4

16-02-2012 дата публикации

Novel Variant Hypocrea Jecorina CBH2 Cellulases

Номер: US20120040435A1

Автор: Bradley Kelemen, Colin Mitchinson, Frits Goedegebuur, Lydia Dankmeyer, Paulien Neefe, Pauline Teunissen, Robert Caldwell, Wolfgang Aehle

Принадлежит: DANISCO US INC

Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.

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Номер записи: 5

08-03-2012 дата публикации

Method of fracturing using mannanohydrolase enzyme breaker

Номер: US20120055670A1

Автор: Charles David Armstrong

Принадлежит: Individual

A thermophilic mannanohydrolase enzyme may be used as an enzyme breaker for fracturing fluids containing hydratable polymers of guar and underivatized guar. The amino acid sequence of the mannanohydrolase is at least 90% homologous to the amino acid sequence of SEQ ID NO:2.

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Номер записи: 6

15-03-2012 дата публикации

Enzymatic Oil-Degumming Method

Номер: US20120064192A1

Автор: Jorn Borch Soe, Mark Turner

Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

A process of enzymatic degumming edible oils, comprising treating edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxyl group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprise one or more of the following amino acid sequences: SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID NO: 16 is also taught.

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Номер записи: 7

22-03-2012 дата публикации

Extracellular yaluronidase from streptomyces koganeiensis

Номер: US20120070441A1

Автор: Giovanni Gennari, Luciano Messina, Salvatore Caruso, Susana Vaccaro

Принадлежит: KETER PLASTIC LTD

The invention relates to Streptomyces koganeiensis ATCC 31394 hyaluronidase having molecular weight of 21.6 kDalton, which has hyaluronidase activity and stability markedly higher than those of the hyaluronidase obtained from such microorganism to date. The invention further relates to a process for the isolation and purification of said hyaluronidase and its use for the preparation of pharmaceutical compositions or as an analytical reagent.

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Номер записи: 8

26-04-2012 дата публикации

Phospholipases, nucleic acids encoding them and methods for making and using them

Номер: US20120100581A1

Автор: Adrian Badillo, Amit Vasavada, Blake G. Sturgis, Charles Isaac, Dan E. Robertson, David Lam, Geoff Hazlewood, Giselle Janssen, Jincai Li, Joel A. Kreps, Mark J. Burk, Nelson R. Barton, Robert C. Brown, Roderick James Fielding, Svetlana Gramatikova, Wilhelmus P. Van Hoek, Xuqiu Tan

Принадлежит: Verenium Corp

The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.

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Номер записи: 9

10-05-2012 дата публикации

Method for production of fermentable sugars from biomass

Номер: US20120115192A1

Автор: Annamma Anil Odaneth, Arvind Mallinath Lali, Jayesh Suman Varavadekar, Pooja Devidas Nagwekar, Prathamesh Chandrashekhar Wadekar, Rajeshwar Dattatray Valte, Sachinkumar Hiraman Birhade, Swapnali Subhash Gujarathi

Принадлежит: CHEMICAL ENGINEERING DEPARTMENT INSTITUTE OF CHEMICAL TECHNOLOGY (DEEMED UNIVERSITY)

A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process.

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Номер записи: 10

10-05-2012 дата публикации

Enhanced cellulase expression in s. degradans

Номер: US20120115235A1

Автор: Steven W. Hutcheson

Принадлежит: University of Maryland at Baltimore

The invention provides organisms and methods of using and making organisms with enhanced cellulase expression.

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Номер записи: 11

31-05-2012 дата публикации

Protein crystallization using molecularly imprinted polymers

Номер: US20120135442A1

Автор: Naomi Esther Chayen, Subrayal Medapati Venkt Reddy

Принадлежит: UNIVERSITY OF SURREY

The invention relates to a method comprising: providing a molecularly imprinted polymer imprinted with a first peptide or protein; exposing said molecularly imprinted polymer to a supersaturated solution of a second peptide or protein; and forming a nucleus of and/or growing a crystal of said second peptide or protein on said molecularly imprinted polymer.

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Номер записи: 12

31-05-2012 дата публикации

Method for Producing Nucleases of a Gram Negative Bacterium While Using a Gram Positive Expression Host

Номер: US20120135498A1

Автор: Stefan Schoenert, Thomas Greiner-Stoeffele

Принадлежит: C Lecta GmbH

A method for producing a nuclease of a gram negative bacterium or a nuclease preparation containing a nuclease of a gram negative bacterium including expression of the nuclease in a gram positive bacterium and subsequent secretion of the nuclease, as well as a nuclease or a nuclease preparation that can be obtained by this method.

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Номер записи: 13

07-06-2012 дата публикации

Methods and Compositions for Treatment of Muscular Dystrophy

Номер: US20120141441A1

Автор: Alfonso P. FARRUGGIO, Christopher Bjornson, Christopher L. Chavez, Chunli Zhao, Hassan Chaib, Jonathan M. Geisinger, Marisa Karow, Michele P. Calos, Tawny Neal

Принадлежит: Leland Stanford Junior University

The present disclosure provides methods for introducing a gene encoding a muscle membrane protein into a cell isolated from a subject to generate a genetically modified cell. The genetically modified cell may be introduced back, e.g., engrafted into the subject. The isolated cell may be additionally modified by introducing into the isolated cell a gene encoding one or more reprogramming transcription factors that induce the cell to form an induced pluripotent stem cell. The genetically modified cell may be differentiated in vitro to form muscle cell precursors before engrafting into the subject. Also provided are compositions comprising autologous cells isolated from a subject which cells comprise a muscle membrane protein gene integrated into a genome attachment site in the genome of the cell. The autologous cell may be an induced pluripotent cell or a mesenchymal stem cell, such as an adipose-derived mesenchymal stem cell (AD-MSC).

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Номер записи: 14

07-06-2012 дата публикации

Targeted integration into the ppp1r12c locus

Номер: US20120142055A1

Автор: David Paschon, Fyodor Urnov, Philip D. Gregory, Phillip Tam, Russell DeKelver

Принадлежит: Sangamo Biosciences Inc

Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.

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Номер записи: 15

07-06-2012 дата публикации

Engineered cleavage half-domains

Номер: US20120142062A1

Автор: Jeffrey C. Miller, Yannick Doyon

Принадлежит: Sangamo Biosciences Inc

Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.

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Номер записи: 16

14-06-2012 дата публикации

Novel protein having b-glucosidase activity and uses thereof

Номер: US20120148706A1

Автор: Fumikazu Yokoyama, Kengo Yokoyama, Nobuko MAZUKA

Принадлежит: Meiji Seika Pharma Co Ltd

By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic β-glucosidase was successfully identified from Acremonium cellulolyticus . Further, a gene corresponding to the identified β-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high β-glucosidase activity.

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Номер записи: 17

14-06-2012 дата публикации

Targeted genomic rearrangements using site-specific nucleases

Номер: US20120149115A1

Автор: Eun Ji Kim, Hyung Joo Lee, Jin Soo Kim

Принадлежит: SNU R&DB FOUNDATION, Toolgen Inc

The present invention relates to a method for genomic DNA rearrangements, and more particularly, to a method for deletion, duplication, inversion, replacement, or rearrangement of genomic DNA using pairs of site-specific nucleases targeting two or more sites in the genome, a cell in which genomic DNA is deleted, duplicated, inverted, replaced, or rearranged by the same method, and a method for expressing the site-specific nucleases in cells. Further, the present invention relates to a method for inserting synthetic DNA molecules into the genome using site-specific nucleases targeting a pre-determined site in the genome, a cell in which DNA insertion occurs by the same method, and a method for expressing the site-specific nucleases in cells.

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Номер записи: 18

21-06-2012 дата публикации

Lipid Production

Номер: US20120151833A1

Автор: Antti Larjo, Matti Karp, Perttu Koskinen, Suvi Myllyntausta, Tommi Aho, Ville Santala, Virpi Kivinen

Принадлежит: Neste Oyj

The present invention relates to a genetically modified Acinetobacter host for lipid production. The Acinetobacter host has been genetically modified to be deficient of one or more of genes A) a gene encoding fatty acyl-CoA reductase (EC1.2.1.n2), wherein said host is capable of increased production of TAGs and/or of total lipids compared to the parent host; and/or B) a gene encoding lipase (EC:3.1.1.3), a gene encoding pyruvate dehydrogenase (EC:1.2.2.2), and/or gene ACIAD 2177, or functional equivalents of any of said genes, wherein said host is capable of increased production of wax esters and/or total lipids compared to the parent host.

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Номер записи: 19

28-06-2012 дата публикации

Proteins for use in human and animal staphyococcus infections

Номер: US20120164126A1

Автор: Anja Philipp, Holger Grallert, Manfred Biebl, Michael Forchheim

Принадлежит: HYGLOS INVEST GMBH

The present invention relates to a polypeptide termed ply_pitti26 comprising the sequence as depicted in SEQ ID NO:1 as well as variants of this polypeptide. Furthermore, the present invention relates to nucleic acids and vectors encoding for said polypeptide and variants thereof as well as host cells comprising these nucleic acids and/or vectors. Finally, the present invention relates to the uses of said polypeptide, variants thereof, nucleic acid sequences, vectors and host cells, in particular for the treatment or prophylaxis of a subject infected by or exposed to Staphylococci.

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Номер записи: 20

28-06-2012 дата публикации

Combinatorial variants of glucoamylase with improved specific activity and/or thermostability

Номер: US20120164695A1

Автор: Casper Vroeman, Martijn Silvan Scheffers, Richard R. Bott, Wolfgang Aehle

Принадлежит: DANISCO US INC

Presently provided are variant glucoamylases displaying altered properties, such as improved thermostability and/or specific activity. Also disclosed are DNA sequences coding for the variants, vectors and host cells incorporating the DNA sequence, enzyme compositions, and methods of using the variants in various applications.

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Номер записи: 21

28-06-2012 дата публикации

Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass

Номер: US20120164696A1

Автор: Attila Andor, Jie Yang, Xiyun Zhang

Принадлежит: Codexis Inc

The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

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Номер записи: 22

28-06-2012 дата публикации

Renewable chemicals and fuels from oleaginous yeast

Номер: US20120164701A1

Автор: Anna Coragliotti, Anthony G. Day, Chung-Soon Im, Donald E. Trimbur, Harrison F. Dillon, Scott Franklin

Принадлежит: Solazyme Inc

The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.

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Номер записи: 23

05-07-2012 дата публикации

Polypeptides of Botryosphaeria Rhodina

Номер: US20120171189A1

Автор: Kirk Matthew Schnorr, Lene Lange, Pernille Uldall Bolvig

Принадлежит: Novozymes AS

The invention relates to functional polypeptides secreted from Botryospaeria rhodina CBS 274.96.

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Номер записи: 24

05-07-2012 дата публикации

Powdery lipase preparation and use thereof

Номер: US20120171736A1

Автор: Satoshi Negishi, Yoshie Yamauchi, Yosuke Nakamura, Yuko Toyama

Принадлежит: Nisshin Oillio Group Ltd

The present invention discloses a powdery lipase preparation in which the particles constituting the powdery lipase have 3,000 to 40,000 pores/mm 2 on the surface thereof, and each pore has a diameter of 0.5 μm to 6 μm. This powdery lipase preparation was able to improve lipase activity without using a soybean powder.

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Номер записи: 25

05-07-2012 дата публикации

Halomonas Strain WDG195-Related Alpha-Amylases, And Methods Of Use, Thereof

Номер: US20120171748A1

Автор: Brian E. Jones, Chris Leeflang, Leo P.M. Van Marrewijk, Marc Kolkman, Melodie Estabrook

Принадлежит: DANISCO US INC

Compositions and methods relating to an alpha-amylase enzyme obtained from Halomonas variabilis WDG195 are described.

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Номер записи: 26

26-07-2012 дата публикации

Methods and compositions for gene correction

Номер: US20120192301A1

Автор: Frank Soldner, Josee Laganiere, Lei Zhang, Rudolf Jaenisch

Принадлежит: Sangamo Therapeutics Inc

Disclosed herein are methods and compositions for correction and/or mutation of genes associated with Parkinson's Disease as well as clones and animals derived therefrom.

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Номер записи: 27

02-08-2012 дата публикации

Heterogenous enzymatic catalyst, preparation method, and use

Номер: US20120196337A1

Автор: Annick Babeau-Garcia, Clement Sanchez, Nicolas Brun, Renal Backov

Принадлежит: Individual

The invention relates to a heterogenous enzymatic catalyst that takes the form of a cellular monolith consisting of a silica or organically modified silica matrix, said monolith including macropores, mesopores, and micropores, said pores being interconnected, and wherein the inner surface of the macropores is functionalized by a coupling agent selected from among silanes, said inner surface moreover having an unpurified enzyme attached thereon by means of a covalent or electrostatic bond. The invention also relates to the method for preparing said catalyst, said method comprising: a first step for preparing a solid silica impression that takes the form of a cellular monolith such as defined above; a second step for functionalizing the inner surface of the macropores via a coupling agent, selected from among silanes, by vacuum-soaking the cellular monolith by dissolving the coupling agent in an organic solvent; and a third step for vacuum-soaking the thus-functionalized monolith by means of an aqueous solution or aqueous dispersion of at least one unpurified enzyme. Finally, the invention relates to the use of such catalyst to carry out catalyzed chemical reactions.

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Номер записи: 28

16-08-2012 дата публикации

Endoglucanase variants

Номер: US20120208235A1

Автор: Ish Kumar Dhawan, Jie Yang, Sachin Patil, Xiyun Zhang

Принадлежит: Codexis Inc

The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.

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Номер записи: 29

16-08-2012 дата публикации

Alpha-amylase variant with altered properties

Номер: US20120208251A1

Автор: Allan Svendsen, Carsten Andersen, Claus von der Osten, Thomas Thisted

Принадлежит: Novozymes AS

The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.

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Номер записи: 30

20-09-2012 дата публикации

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

Номер: US20120237979A1

Автор: Junxin Duan, Kirk Matthew Schnorr, Wenping Wu

Принадлежит: Novozymes AS

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 31

04-10-2012 дата публикации

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

Номер: US20120252068A1

Автор: Alfredo Lopez de Leon, Hanshu Ding, Kimberly Brown

Принадлежит: Novozymes Inc

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 32

04-10-2012 дата публикации

Alpha-Amylase Variants

Номер: US20120252095A1

Автор: Allan Svendsen, Carsten Andersen, Henrik Bisgaard-Frantzen, Sóeren Kjaerulff

Принадлежит: Novozymes AS

The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.

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Номер записи: 33

04-10-2012 дата публикации

Optimized messenger rna

Номер: US20120252117A1

Автор: Allan M. Miller, Douglas A. Treco, Richard F Selden

Принадлежит: Individual

The present invention is directed to a synthetic nucleic acid sequence which encodes a protein wherein at least one non-common codon or less-common codon is replaced by a common codon. The synthetic nucleic acid sequence can include a continuous stretch of at least 90 codons all of which are common codons.

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Номер записи: 34

11-10-2012 дата публикации

Chimeric bacteriophage lysin with activity against staphylococci bacteria

Номер: US20120258088A1

Автор: Anu Daniel, Chad Euler, Vincent A. Fischetti

Принадлежит: ROCKEFELLER UNIVERSITY

The present disclosure relates to chimeric bacteriophage lysins useful for the identification and/or reduction of staphylococcal populations. For example, a chimeric bacteriophage lysin was engineered and shown to effectively kill all strains of staphylococci tested including antibiotic resistant methicillin-resistant S. Aureus and VISA.

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Номер записи: 35

11-10-2012 дата публикации

Method for Incorporating Internal Polar and Ionizable Groups in Proteins

Номер: US20120258518A1

Автор: Bertrand E. Garcia-Moreno, Daniel G. ISOM

Принадлежит: JOHNS HOPKINS UNIVERSITY

Internal polar and ionizable groups are essential for enzymatic catalysis, proton transport, redox reactions, and many other functional properties of proteins. To engineer novel enzymes or to modify the function of existing ones, and to build switches that can be used to modify the stability of proteins in response to changes in pH, it is necessary to introduce polar or ionizable groups or to modify the properties of existing ones. However, internal polar and ionizable groups usually destabilize proteins. The disclosure provides new methods that allow the introduction of polar and ionizable groups into the interior of proteins, as well as new methods for improving the accuracy of pK a of an internal amino acid of a protein, and methods for mapping the folding free energy landscape of a protein.

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Номер записи: 36

11-10-2012 дата публикации

Polypeptides having Beta-Glucosidase activity and polynucleotides encoding same

Номер: US20120258520A1

Автор: Kristian Krogh, Paul Harris

Принадлежит: Novozymes Inc

The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

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Номер записи: 37

11-10-2012 дата публикации

Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same

Номер: US20120260371A1

Автор: Eric Abbate, Kimberly Brown, Nikolaj Spodsberg

Принадлежит: Novozymes AS, Novozymes Inc

The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 38

18-10-2012 дата публикации

Novel ginsenoside glycosidase derived from the genus terrabacter, and use thereof

Номер: US20120264167A1

Автор: Dong Shan AN, Hyung Gwan LEE, Song Gun KIM, Sun Chang Kim, Sung Taik LEE, Wan Taek IM

Принадлежит: Korea Research Institute of Bioscience and Biotechnology KRIBB

The present invention relates to a novel ginsenoside glycosidase protein derived from the genus Terrabacter , the protein having an activity of converting protopanaxadiol (PPD)-type saponins into highly active substances, which can be absorbed inside the body, by selective hydrolysis of a particular bond of ginsenoside. More specifically, the present invention relates to an amino acid sequence of the protein, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector, and a method for producing ginsenoside glycosidase derived from the genus Terrabacter by culturing the transformant, a method for converting PPD-type major saponins into the minor saponin forms using the protein, and a composition for converting PPD-type saponins into soluble saponins, comprising the protein as an active component.

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Номер записи: 39

18-10-2012 дата публикации

Dnase expression in recombinant host cells

Номер: US20120264169A1

Автор: Jon Martin Persson, Michael Dolberg Rasmussen

Принадлежит: Novozymes AS

The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.

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Номер записи: 40

25-10-2012 дата публикации

Method For Preparing Maltogenic Alpha-Amylase Variants

Номер: US20120270253A1

Автор: Allan Svendsen, Carsten Andersen, Joel Cherry, Lars Beier, Torben Peter Frandsen

Принадлежит: Novozymes AS

The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.

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Номер записи: 41

25-10-2012 дата публикации

Variant humicola grisea cbh1.1

Номер: US20120270270A1

Автор: Colin Mitchinson, Edmund Larenas, Frits Goedegebuur, Peter Gualfetti

Принадлежит: DANISCO US INC

Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.

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Номер записи: 42

25-10-2012 дата публикации

Method for constructing chimeric rat using rat embryonic stem cells

Номер: US20120272349A1

Автор: Masaki Kawamata, Takahiro Ochiya

Принадлежит: DS Pharma Biomedical Co Ltd, National Cancer Center Japan

The present invention provides a preparation method of a chimeric embryo and a chimeric rat, which is characterized by contacting a rat pluripotent stem cell and a host embryo in the presence of an ES cell differentiation inhibitor. The method includes (a) a step for contacting a fertilized host embryo collected from a female rat and a rat pluripotent stem cell in the presence of an ES cell differentiation suppressant, and (b) a step for culturing the host embryo in contact with the rat pluripotent stem cell to form a chimeric embryo.

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Номер записи: 43

01-11-2012 дата публикации

Therapeutic ribonucleases

Номер: US20120276077A1

Автор: John Kink, Laura Strong, Tony Klink

Принадлежит: Quintessence Biosciences Inc

The present invention relates to the use of ribonucleases (RNases) in the treatment or prevention of disease.

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Номер записи: 44

01-11-2012 дата публикации

Homologous recombination in the oocyte

Номер: US20120276537A1

Автор: Melanie Meyer, Ralf KÜHN, Wolfgang Wurst

Принадлежит: Individual

The present invention relates to a method of modifying a target sequence in the genome of a mammalian or avian oocyte by homologous recombination with a donor nucleic acid sequence, the method comprising the steps (a) introducing into the oocyte a zinc finger nuclease or a nucleic acid molecule encoding the zinc finger nuclease in expressible form, wherein the zinc finger nuclease specifically binds within the target sequence and introduces a double strand break within the target sequence; and (b) introducing a nucleic acid molecule into the oocyte, wherein the nucleic acid molecule comprises the donor nucleic acid sequence and regions homologous to the target sequence. The present invention further relates to a method of producing a non-human mammal or an avian carrying a modified target sequence in its genome.

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Номер записи: 45

01-11-2012 дата публикации

Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials

Номер: US20120276595A1

Автор: Amy D. Liu, Bradley R. Kelemen, Luis G. Cascao-Pereira, Thijs Kaper

Принадлежит: DANISCO US INC

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.

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Номер записи: 46

08-11-2012 дата публикации

Alpha-Amylase Mutants

Номер: US20120283164A1

Автор: Allan Svendsen, Bjarne Ronfeldt Nielsen, Helle Outtrup, Henrik Bisgård-Frantzen, Lisbeth Hedegaard, Torben Vedel Borchert, Vibeke Skovgaard Neilsen

Принадлежит: Novozymes AS

The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca 2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca 2+ concentrations (relative to the parent).

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Номер записи: 47

08-11-2012 дата публикации

Chimeric Endonucleases and Uses Thereof

Номер: US20120284877A1

Автор: Andrea Hlubek, Christian Biesgen, Hans Wolfgang HÖFFKEN

Принадлежит: BASF Plant Science Co GmbH

The invention relates to chimeric endonucleases, comprising a endonuclease and a heterologous DNA binding domain comprising one or more Zn 2 C 6 zinc fmgers, as well as methods of targeted integration, targeted deletion or targeted mutation of polynucleotides using chimeric endonucleases.

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Номер записи: 48

29-11-2012 дата публикации

Novel heparanase splice variant

Номер: US20120301475A1

Автор: Gad S. Cojocaru, Iris Hecht, Israel Vlodavsky, Michal Ayalon-Soffer, Neta Ilan, Ronen Shemesh, Sergey Nemzer, Tomer Zekharya, Uri Barash, Zurit Levine

Принадлежит: Compugen Ltd

The present invention, in at least some aspects, is of splice variants of heparanase, as well as diagnostic kits and methods of use, and therapeutic agents and methods of use based thereon, and antibodies specifically binding thereof.

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Номер записи: 49

29-11-2012 дата публикации

Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof

Номер: US20120304321A1

Автор: Emmanuel Lacroix, Frederic Paques, Jean-Charles Epinat, Patrick Chames, Phillippe Duchateau, Sylvain Arnould

Принадлежит: CELLECTIS SA

A monomer of an I-CreI meganuclease variant wherein said monomer when in dimeric form binds and cleaves DNA.

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Номер записи: 50

06-12-2012 дата публикации

Methods and Compositions for Gene Inactivation

Номер: US20120308528A1

Автор: Dale Ando, Gary Ka Leong Lee, Michael C. Holmes, Yann Jouvenot

Принадлежит: Sangamo Biosciences Inc

Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.

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Номер записи: 51

27-12-2012 дата публикации

Polypeptides having endoglucanase activity and polynucleotides encoding same

Номер: US20120331587A1

Автор: Elena Vlasenko, Kristian Bertel Romer Morkeberg Krogh, Paul Harris, Soren Flensted Lassen

Принадлежит: Novozymes AS, Novozymes Inc

The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

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Номер записи: 52

03-01-2013 дата публикации

Cleaning compositions comprising amylase variants reference to a sequence listing

Номер: US20130000055A1

Автор: Allan Svendsen, Annette Helle Johansen, Carsten Andersen, Esben Peter Friis, Frank Winther Rasmussen, Jens Oebro, Lars Beier, Lindsay Suzanne Bewick, Liv Spaangner Christiansen, Mads Bjoernvad, Michael Skjoet, Michelle Jackson, Miguel Duarte Guilherme Pereira Toscano, Philip Frank Souter, Signe Eskildsen Larsen, Svend Kaasgaard

Принадлежит: Procter and Gamble Co

The present invention relates to cleaning compositions comprising variants of an alpha-amylase and methods of treating surfaces such as textiles with aqueous liquor comprising such compositions, especially at low temperatures.

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Номер записи: 53

03-01-2013 дата публикации

Ts-23 alpha-amylase variants with altered properties

Номер: US20130005029A1

Автор: Brain E. Jones, Casper Vroemen, Chris Leeflang, Claudine Chang, Clement Choy, James T. Kellis, Jr., Luis G. Cascao-Pereira, Marc Kolkman, Melodie Estabrook, Walter Weyler

Принадлежит: DANISCO US INC

Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.

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Номер записи: 54

10-01-2013 дата публикации

Cytotoxic ribonuclease variants

Номер: US20130011904A1

Автор: George N. Phillips, JR., Jason G. McCoy, R. Jeremy Johnson, Ronald T. Raines

Принадлежит: WISCONSIN ALUMNI RESEARCH FOUNDATION

Cytotoxic variants of human ribonuclease 1 (RNase 1) identified through analysis of the interaction between RNase 1 and the human ribonuclease inhibitor (hRI) as defined by the three dimensional (3-D) atomic structure of the RNase1 hRI complex are disclosed. Also disclosed is the 3-D structure of the hRI·RNase 1 complex and methods for designing the RNase 1 variants.

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Номер записи: 55

14-02-2013 дата публикации

Treatment of Pompe's Disease

Номер: US20130039901A1

Автор: David P. Meeker, Edna H.G. Venneker, Johannes B.M.M. Van Bree

Принадлежит: Genzyme Therapeutic Products LP

The invention provides methods of treating Pompe's disease using human acid alpha glucosidase. A preferred treatment regime comprises administering greater than 10 mg/kg body weight per week to a patient.

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Номер записи: 56

14-02-2013 дата публикации

Cellobiohydrolase Variants and Polynucleotides Encoding Same

Номер: US20130040346A1

Автор: Mark Wogulis

Принадлежит: Novozymes Inc

The present invention relates to variants of a parent cellobiohydrolase. The present invention also relates to polynucleotides encoding the cellobiohydrolase variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the cellobiohydrolase variants.

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Номер записи: 57

21-02-2013 дата публикации

Novel Beta-Glucosidase and Uses thereof

Номер: US20130045510A1

Автор: Chen-Wei Li, Hsion-Wen Kuo, Ng I-Son Wu, Su-May Yu, Tuan-Hua David Ho, Yu-Ming Ju

Принадлежит: Academia Sinica

A novel beta-glucosidase and nucleic acids encoding the beta-glucosidase. Also disclosed are cells, compositions, and methods relating to using the beta-glucosidase to convert ligocellulosic material to fermentable sugars.

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Номер записи: 58

28-02-2013 дата публикации

Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity

Номер: US20130052713A1

Автор: David ELGART, Dipnath Baidyaroy, Jie Yang, John H. Grate, Jungjoo YOON, Kripa Rao, Xiyun Zhang

Принадлежит: Codexis Inc

The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.

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Номер записи: 59

28-02-2013 дата публикации

Yeast strains producing mammalian-like complex n-glycans

Номер: US20130053550A1

Автор: Steven Christian Jozef Geysens, Wouter Vervecken

Принадлежит: Oxyrane UK Ltd

Described herein are methods and genetically engineered fungal cells useful for producing target molecules containing mammalian-like complex N-glycans or containing intermediates in a mammalian glycosylation pathway.

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Номер записи: 60

07-03-2013 дата публикации

Polypeptide shaving transgalactosylating activity

Номер: US20130059034A1

Автор: Charlotte Horsmans Poulsen, Morten Krog Larsen

Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

The present invention relates to polypeptides, specifically polypeptides having transgalactosylating activity and nucleic acids encoding these, and their uses in e.g. dairy product.

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Номер записи: 61

14-03-2013 дата публикации

Coatings Containing Polymer Modified Enzyme For Stable Self-Cleaning Of Organic Stains

Номер: US20130065291A1

Автор: Andreas Buthe, Hongfei Jia, Liting Zhang, Masahiko Ishii, Minjuan Zhang, PING Wang, Songtao Wu, Xueyan Zhao

Принадлежит: Department of Bioproducts and Biosystems Engineering of University of Minnesota, Toyota Motor Corp, Toyota Motor Engineering and Manufacturing North America Inc

Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

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Номер записи: 62

21-03-2013 дата публикации

RNA Interferases and Methods of Use Thereof

Номер: US20130071374A1

Автор: Inouye Masayori, Zhang Junjie, Zhang Yong Long

Принадлежит: UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY

The present invention is directed to the discovery of a novel family of enzymes designated herein as mRNA interferases that exhibit endoribonuclease activity. The novel finding of the present inventors, therefore, presents new applications for which mRNA interferase nucleic and amino acid sequences, and compositions thereof may be used to advantage. The invention also encompasses screening methods to identify compounds/agents capable of modulating mRNA interferase activity and methods for using such compounds/agents. Also provided is a kit comprising mRNA interferase nucleic and/or amino acid sequences, mRNA interferase activity compatible buffers, and instruction materials. 1. A method for detecting an activity of an mRNA interferase or functional fragment thereof , wherein said activity is endoribonuclease activity , said method comprising:(a) providing a nucleic acid sequence encoding said mRNA interferase or a functional fragment thereof;(b) expressing said nucleic acid sequence;(c) incubating the expressed nucleic acid sequence of step (b) with an endoribonuclease substrate; and(d) measuring cleavage of said substrate, wherein cleavage of said substrate indicates endoribonuclease activity and provides means to detect endoribonuclease activity of said mRNA interferase or a functional fragment thereof.2. A method for screening to identify an agent capable of modulating an activity of an mRNA interferase or functional fragment thereof , wherein said activity is endoribonuclease activity , said method comprising:(a) providing a nucleic acid sequence encoding said mRNA interferase or a functional fragment thereof;(a) expressing said nucleic acid sequence;(b) incubating the expressed nucleic acid sequence of step (b) with an endoribonuclease substrate under conditions capable of promoting endoribonuclease activity;(d) adding at least one agent to determine if it is capable of modulating endoribonuclease activity of said mRNA interferase or functional fragment thereof ...

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Номер записи: 63

28-03-2013 дата публикации

Polypeptides having xylanase activity and polynucleotides encoding same

Номер: US20130078672A1

Автор: Hanshu Ding, Junxin Duan, Lan Tang, Ye Liu

Принадлежит: Novozymes AS, Novozymes Inc

The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 64

28-03-2013 дата публикации

Method for protein production in filamentous fungi

Номер: US20130078674A1

Автор: Hakkinen Mari, Pakula Tiina, Penttilä Merja, Saloheimo Markku, Vitikainen Marika, Westerholm-Parvinen Ann

Принадлежит: TEKNOLOGIAN TUTKIMUSKESKUS VTT

The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host. 1. A method to genetically modify a filamentous fungus host for improved protein production , said method comprising:{'i': Trichoderma, Aspergillus, Fusarium, Neurospora, Talaromyces, Phanerochaete, Chrysosporium', 'Penicillium, 'genetically modifying a filamentous fungus host to overexpress with increased amount or activity, or to be deficient with reduced or lacking amount or activity, of one or more genes selected from the group consisting of genes tre77513 (SEQ ID NO:1), tre80291 (SEQ ID NO:2), tre41573 (SEQ ID NO:3), tre74765 (SEQ ID NO:4), and tre64608 (SEQ ID NO:5); or of the closest homologue of at least one of said genes in or ; or of a fragment or derivative of any of said genes or other nucleotide sequence hybridizing under stringent conditions to at least one of said genes or said homologues, said host being capable of increased or decreased production of cellulase, hemicellulase, other proteins involved in degradation of lignocellulosic material and/or other proteins as compared to the parental strain.'}2Trichoderma, Aspergillus, Fusarium, Neurospora, Talaromyces, Phanerochaete, ChrysosporiumPenicillium. The method according to claim 1 , wherein the filamentous fungus host is genetically modified to overexpress one or more genes selected from the group consisting of genes tre77513 (SEQ ID NO:1) claim 1 , tre80291 (SEQ ID NO:2) claim 1 , tre41573 (SEQ ID NO:3) claim 1 , ...

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Номер записи: 65

04-04-2013 дата публикации

Method for improved protein production in filamentous fungi

Номер: US20130084604A1

Автор: Ann Westerholm-Parvinen, Mari Häkkinen, Marika Vitikainen, Markku Saloheimo, Merja Penttilä, Tiina Pakula

Принадлежит: Valtion teknillinen tutkimuskeskus

The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.

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Номер записи: 66

11-04-2013 дата публикации

Family 44 Xyloglucanases

Номер: US20130086755A1

Автор: Jorgensen Per Lina, Schnorr Kirk, Schulein Martin

Принадлежит: NOVOZYMES A/S

The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus especially from a strain of or sp. The xyloglucanases exhibit high performance in conventional detergent compositions. 121-. (canceled)22. An isolated polypeptide having xyloglucanase activity , which comprises an amino acid sequence which is at least 85% identical with the sequence of amino acids 40-559 of SEQ ID NO: 2.23Bacillus/Lactobacillus. The polypeptide of claim 23 , which is obtained from the subdivision.24Paenibacillus.. The polypeptide of claim 23 , which is obtained from a species of25Paenibacillus polymyxa.. The polypeptide of claim 24 , which is obtained from26. A detergent composition comprising a polypeptide of and a surfactant.27. A process for washing a fabric claim 22 , comprising treating the fabric during a washing cycle of a machine washing process with a washing solution which comprises a polypeptide of . This application is a divisional of U.S. application Ser. No. 13/287,166 filed on Nov. 2, 2011, now allowed, which is a divisional of U.S. application Ser. No. 12/779,112 filed on May 13, 2010, now U.S. Pat. No. 8,071,351, which is a continuation of U.S. application Ser. No. 11/970,559 filed on Jan. 8, 2008, now abandoned, which is a divisional of application Ser. No. 10/896,555 filed Jul. 22, 2004, now U.S. Pat. No. 7,361,736, which is a continuation of U.S. application Ser. No. 09/784,554 filed Feb. 16, 2001, now U.S. Pat. No. 6,815,192, which claims priority of Danish application no. PA 2000 00291 filed Feb. 24, 2000 and U.S. provisional application No. 60/185,317 filed Feb. 28, 2000, the contents of which are incorporated herein by reference.This application contains information in the form of a sequence listing, which is submitted on a data carrier accompanying this application. The contents of the data carrier are fully incorporated ...

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Номер записи: 67

18-04-2013 дата публикации

RECOMBINANT HUMAN NAGLU PROTEIN AND USES THEREOF

Номер: US20130095092A1

Автор: Leavitt Markley C., Quinn Anthony, Xia Zhinan

Принадлежит: Synageva Biopharma Corp.

The present invention provides compositions comprising an isolated mixture of recombinant human NaGlu proteins in which a substantial amount of the NaGlu proteins in the mixture has increased levels of phosphorylated mannose that confer the proteins to be efficiently internalized into human cells. The present invention also provides methods of producing such mixture of NaGlu proteins, vectors used in transgenesis and expression, host cells harboring such vectors, and methods of isolating and purifying the mixture of NaGlu proteins. The invention further provides methods of treating NaGlu associated diseases. 1. A composition comprising an isolated mixture of recombinant human N-acetyl-alpha-D-glucosaminidase (rhNaGlu) comprising the amino acid sequence 24-743 of SEQ ID NO:1 , wherein at least 10% of said rhNaGlu in said mixture comprises at least one glycan structure having mannose-6-phosphate (M6P).2. The composition of claim 1 , wherein said rhNaGlu having M6P is capable of being taken up into a mammalian cell deficient in NaGlu such that internalized rhNaGlu restores at least 50% of normal NaGlu activity observed in a wild-type mammalian cell of the same type.3. The composition of claim 2 , wherein said rhNaGlu contains at least 1 mole of M6P per mole of protein.4. The composition of claim 3 , wherein said rhNaGlu contains about 3 moles of M6P per mole of protein.5. The composition of claim 2 , wherein said mammalian cell deficient in NaGlu is a human cell.6. The composition of claim 2 , wherein said rhNaGlu is effectively delivered to the brain of a mammal having NaGlu deficiency when systemically administered.7. The composition of claim 6 , wherein said rhNaGlu is effectively delivered to the brain of a mammal having NaGlu deficiency when intravenously administered.8. The composition of claim 2 , wherein said rhNaGlu is effectively delivered to the brain of a mammal having NaGlu deficiency when administered intrathecally.9. The composition of claim 2 , wherein ...

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Номер записи: 68

18-04-2013 дата публикации

Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof

Номер: US20130095553A1

Автор: Alrik Pieter Los, Margot Elisabeth Francoise Schooneveld-Bergmans, Wilbert Herman Marie Heijne

Принадлежит: DSM IP ASSETS BV

The invention relates to a polypeptide which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

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Номер записи: 69

25-04-2013 дата публикации

VARIANTS OF GLUCOAMYLASE

Номер: US20130102035A1

Автор: Aehle Wolfgang, Bott Richard R., Degn Peter Edvard, Petersen Elin, Scheffers Martijn Silvan, Vroemen Casper Willem

Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells. 34-. (canceled)5. The glucoamylase variant according to claim 1 , wherein the glucoamylase variant has at least 85% claim 1 , 90% claim 1 , 95% claim 1 , 98% claim 1 , or 99.5% sequence identity with SEQ ID NO: 1 or 2.67-. (canceled)8. The glucoamylase variant of claim 1 , comprising SEQ ID NO: 1098 or SEQ ID NO: 1099.9. (canceled)10. The glucoamylase variant according to claim 1 , wherein the glucoamylase variant has a starch binding domain that has at least 96% claim 1 , 97% claim 1 , 98% claim 1 , 99% claim 1 , or 99.5% sequence identity with the starch binding domain of SEQ ID NO: 1 claim 1 , 2 claim 1 , 11 claim 1 , 385 claim 1 , 386 claim 1 , 387 claim 1 , 388 claim 1 , 389 claim 1 , or 390.11. The glucoamylase variant according to claim 1 , wherein the glucoamylase variant has a catalytic domain that has at least 80% claim 1 , 85% claim 1 , 90% claim 1 , 95% claim 1 , or 99.5% sequence identity with the catalytic domain of SEQ ID NO: 1 claim 1 , 2 claim 1 , 3 claim 1 , 5 claim 1 , 6 claim 1 , 7 claim 1 , 8 claim 1 , or 9.1217-. (canceled)18. The glucoamylase variant according to claim 1 , which glucoamylase exhibit a reduced ratio between isomaltose synthesis and starch hydrolysis activity (IS/SH ratio) as compared to the parent glucoamylase.19. (canceled)20. The glucoamylase variant according to claim 1 , which glucoamylase exhibit an enhanced real degree of fermentation as compared to the parent glucoamylase.2125-. (canceled)26. A polynucleotide encoding a glucoamylase variant according to .27. A vector comprising the ...

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Номер записи: 70

02-05-2013 дата публикации

THERMOSTABLE XYLANASE FOR THE SELECTIVE HYDROLYSIS OF PENTOSE-CONTAINING POLYSACCHARIDES

Номер: US20130109062A1

Автор: Brück Thomas, Fridjonsson Olafur H., Hreggvidsson Gudmundur O., Kettling Ulrich, Kohl Andreas, Koltermann Andre, Rarbach Markus, Reisinger Christoph, Unterstrasser Isabel

Принадлежит: SÜD-CHEMIE AG

The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicellulose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof. 1. A polypeptide having xylanase activity which maintains at least 80% of its xylanase activity after 72 hours incubation in 50 mM phosphate buffer at 70° C.2. The polypeptide according to claim 1 , wherein the polypeptide yields xylose and glucose in a weight ratio of at least 10:1 when subjecting wheat straw to the polypeptide at pH 5 and 45° C.3. The polypeptide according to claim 1 , wherein the polypeptide shows optimum xylanase activity in the pH range of 5-6.4. The polypeptide according to claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 70% claim 1 , sequence identity to the SEQ ID No: 1.5. The polypeptide according to claim 4 , wherein the amino acid sequence of the polypeptide has the sequence as defined by SEQ ID No: 1 or SEQ ID No: 2 or SEQ ID No: 3 or SEQ ID No: 4 claim 4 , or a sequence as defined by SEQ ID No: 1 or SEQ ID No: 2 or SEQ ID No: 3 or SEQ ID No: 4 wherein any 1 to 30 amino acid residues are substituted claim 4 , deleted claim 4 , or inserted.6. The polypeptide according to claim 1 , wherein the polypeptide maintains at least 80% of its xylanase activity after having been subjected to trypsin at pH 7.8 and 50° C. for 1 hour.7. The polypeptide according to claim 1 , wherein the xylanase shows an optimum activity for the hydrolysis of xylose from wheat straw in the temperature range between 45 and 80° C.8. The polypeptide according to claim 1 , which is expressed and ...

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Номер записи: 71

02-05-2013 дата публикации

METHODS AND MEANS TO MODIFY A PLANT GENOME AT A NUCLEOTIDE SEQUENCE COMMONLY USED IN PLANT GENOME ENGINEERING

Номер: US20130111620A1

Автор: DHalluin Kathleen

Принадлежит: BAYER CROPSCIENCE NV

Methods and means are provided to modify in a targeted manner the plant genome of transgenic plants comprising chimeric genes wherein the chimeric genes have a DNA element commonly used in plant molecular biology. Re-designed meganucleases to cleave such an element commonly used in plant molecular biology are provided. 2. The method according to claim 1 , wherein said predefined site comprises the nucleotide sequence of SEQ ID No 1.3. The method according to or claim 1 , wherein said 3′ end termination and polyadenylation region of gene 7 comprises the nucleotide sequence of SEQ ID No. 264. The method according to any one of claim 1 , claim 1 , or claim 1 , wherein said pair of meganucleases obligatory forms heterodimers or wherein said meganuclease is a single chain meganuclease comprising two domains derived from I-CreI covalently connected by a linker.74. The method according to any one of claim 1 , claim 1 , or claim 1 , wherein said pair of meganucleases comprises the amino acid sequence of SEQ ID No. 4 and SEQ ID No. 5 claim 1 , respectively claim 1 , or wherein said pair of meganucleases is encoded by a nucleic acid comprising the nucleotide sequence of SEQ ID No. 3 from nucleotide position 2004 to nucleotide position 2525 or to 2522 claim 1 , or the nucleotide sequence of SEQ ID No. 3 from nucleotide position 4885 to nucleotide position 5405 or to 5403 claim 1 , or wherein said single chain meganuclease comprises the amino acid sequence of SEQ ID No. 7 or said single chain meganuclease comprises an amino acid sequence comprising the amino acid sequence of SEQ ID No. 7 from position 1 to 167 and from position 206 to 362 claim 1 , or wherein said single chain meganuclease is encoded by a nucleotide sequence comprising the nucleotide sequence of SEQ ID No. 6 from position 1267 to position 1602 or to 1605 and from position 1796 or from 1795 to position 2544 or to 2541 claim 1 , or said single chain meganuclease is encoded by a nucleotide sequence comprising the ...

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Номер записи: 72

09-05-2013 дата публикации

High Fidelity Restriction Endonucleases

Номер: US20130115676A1

Автор: Chan Siu-hong, Guan Shengxi, Quimby Aine, Sun Dapeng, Wei Hua, Xu Shuang-Yong, Zhang Penghua, Zhu Zhenyu

Принадлежит: NEW ENGLAND BIOLABS, INC.

Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. 1. A composition , comprising: a restriction endonuclease having at least one artificially introduced mutation and an overall fidelity index (FI) improvement factor of at least 2 , the restriction endonuclease being capable of cleaving a substrate with at least a similar cleavage activity to that of the restriction endonuclease absent the artificially introduced mutation , in a predetermined buffer , wherein the artificially introduced mutation is the product of at least one of a targeted mutation , saturation mutagenesis , or a mutation introduced through a PCR amplification procedure.2. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with an oppositely charged residue at a target site in the restriction endonuclease.3. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with a residue selected from a Phenylalanine and an Alanine at a target site in the restriction endonuclease.4. A composition claim 1 , according to claim 1 , wherein the restriction enzyme absent the at least one artificially introduced mutation is selected from the group consisting of: BamHI claim 1 , EcoRI claim 1 , ScaI claim 1 , SalI claim 1 , SphI claim 1 , PstI claim 1 , NcoI claim 1 , NheI claim 1 , SspI claim 1 , NotI claim 1 , SacI claim 1 , PvuII claim 1 , MfeI claim 1 , HindIII claim 1 , SbfI claim 1 , EagI claim ...

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Номер записи: 73

09-05-2013 дата публикации

High Fidelity Restriction Endonucleases

Номер: US20130115677A1

Автор: Chan Siu-hong, Guan Shengxi, Quimby Aine, Sun Dapeng, Wei Hua, Xu Shuang-Yong, Zhang Penghua, Zhu Zhenyu

Принадлежит: NEW ENGLAND BIOLABS, INC.

Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. 1. A composition , comprising: a restriction endonuclease having at least one artificially introduced mutation and an overall fidelity index (FI) improvement factor of at least 2 , the restriction endonuclease being capable of cleaving a substrate with at least a similar cleavage activity to that of the restriction endonuclease absent the artificially introduced mutation , in a predetermined buffer , wherein the artificially introduced mutation is the product of at least one of a targeted mutation , saturation mutagenesis , or a mutation introduced through a PCR amplification procedure.2. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with an oppositely charged residue at a target site in the restriction endonuclease.3. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with a residue selected from a Phenylalanine and an Alanine at a target site in the restriction endonuclease.4. A composition claim 1 , according to claim 1 , wherein the restriction enzyme absent the at least one artificially introduced mutation is selected from the group consisting of: BamHI claim 1 , EcoRI claim 1 , ScaI claim 1 , SalI claim 1 , SphI claim 1 , PstI claim 1 , NcoI claim 1 , NheI claim 1 , SspI claim 1 , NotI claim 1 , SacI claim 1 , PvuII claim 1 , MfeI claim 1 , HindIII claim 1 , SbfI claim 1 , EagI claim ...

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Номер записи: 74

09-05-2013 дата публикации

High Fidelity Restriction Endonucleases

Номер: US20130115678A1

Автор: Aine Quimby, Dapeng Sun, Hua Wei, Penghua Zhang, Shengxi Guan, Shuang-Yong Xu, Siu-Hong Chan, Zhenyu Zhu

Принадлежит: New England Biolabs Inc

Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.

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Номер записи: 75

09-05-2013 дата публикации

High Fidelity Restriction Endonucleases

Номер: US20130115679A1

Автор: Chan Siu-hong, Guan Shengxi, Quimby Aine, Sun Dapeng, Wei Hua, Xu Shuang-Yong, Zhang Penghua, Zhu Zhenyu

Принадлежит: NEW ENGLAND BIOLABS, INC.

Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. 1. A composition , comprising: a restriction endonuclease having at least one artificially introduced mutation and an overall fidelity index (FI) improvement factor of at least 2 , the restriction endonuclease being capable of cleaving a substrate with at least a similar cleavage activity to that of the restriction endonuclease absent the artificially introduced mutation , in a predetermined buffer , wherein the artificially introduced mutation is the product of at least one of a targeted mutation , saturation mutagenesis , or a mutation introduced through a PCR amplification procedure.2. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with an oppositely charged residue at a target site in the restriction endonuclease.3. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with a residue selected from a Phenylalanine and an Alanine at a target site in the restriction endonuclease.4. A composition claim 1 , according to claim 1 , wherein the restriction enzyme absent the at least one artificially introduced mutation is selected from the group consisting of: BamHI claim 1 , EcoRI claim 1 , ScaI claim 1 , SalI claim 1 , SphI claim 1 , PstI claim 1 , NcoI claim 1 , NheI claim 1 , SspI claim 1 , NotI claim 1 , SacI claim 1 , PvuII claim 1 , MfeI claim 1 , HindIII claim 1 , SbfI claim 1 , EagI claim ...

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Номер записи: 76

09-05-2013 дата публикации

High Fidelity Restriction Endonucleases

Номер: US20130115680A1

Автор: Chan Siu-hong, Guan Shengxi, Quimby Aine, Sun Dapeng, Wei Hua, Xu Shuang-Yong, Zhang Penghua, Zhu Zhenyu

Принадлежит: NEW ENGLAND BIOLABS, INC.

Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. 1. A composition , comprising: a restriction endonuclease having at least one artificially introduced mutation and an overall fidelity index (FI) improvement factor of at least 2 , the restriction endonuclease being capable of cleaving a substrate with at least a similar cleavage activity to that of the restriction endonuclease absent the artificially introduced mutation , in a predetermined buffer , wherein the artificially introduced mutation is the product of at least one of a targeted mutation , saturation mutagenesis , or a mutation introduced through a PCR amplification procedure.2. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with an oppositely charged residue at a target site in the restriction endonuclease.3. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with a residue selected from a Phenylalanine and an Alanine at a target site in the restriction endonuclease.4. A composition claim 1 , according to claim 1 , wherein the restriction enzyme absent the at least one artificially introduced mutation is selected from the group consisting of: BamHI claim 1 , EcoRI claim 1 , ScaI claim 1 , SalI claim 1 , SphI claim 1 , PstI claim 1 , NcoI claim 1 , NheI claim 1 , SspI claim 1 , NotI claim 1 , SacI claim 1 , PvuII claim 1 , MfeI claim 1 , HindIII claim 1 , SbfI claim 1 , EagI claim ...

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Номер записи: 77

09-05-2013 дата публикации

High Fidelity Restriction Endonucleases

Номер: US20130115681A1

Автор: Chan Siu-hong, Guan Shengxi, Quimby Aine, Sun Dapeng, Wei Hua, Xu Shuang-Yong, Zhang Penghua, Zhu Zhenyu

Принадлежит: NEW ENGLAND BIOLABS, INC.

Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. 1. A composition , comprising: a restriction endonuclease having at least one artificially introduced mutation and an overall fidelity index (FI) improvement factor of at least 2 , the restriction endonuclease being capable of cleaving a substrate with at least a similar cleavage activity to that of the restriction endonuclease absent the artificially introduced mutation , in a predetermined buffer , wherein the artificially introduced mutation is the product of at least one of a targeted mutation , saturation mutagenesis , or a mutation introduced through a PCR amplification procedure.2. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with an oppositely charged residue at a target site in the restriction endonuclease.3. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with a residue selected from a Phenylalanine and an Alanine at a target site in the restriction endonuclease.4. A composition claim 1 , according to claim 1 , wherein the restriction enzyme absent the at least one artificially introduced mutation is selected from the group consisting of: BamHI claim 1 , EcoRI claim 1 , ScaI claim 1 , SalI claim 1 , SphI claim 1 , PstI claim 1 , NcoI claim 1 , NheI claim 1 , SspI claim 1 , NotI claim 1 , SacI claim 1 , PvuII claim 1 , MfeI claim 1 , HindIII claim 1 , SbfI claim 1 , EagI claim ...

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Номер записи: 78

09-05-2013 дата публикации

Derivatisation of erythropoietin (epo)

Номер: US20130116176A1

Автор: Gregory Gregoriadis, Norbert Oskar Rumpf, Peter Laing, Sanjay Jain

Принадлежит: Lipoxen Technologies Ltd

The present invention relates to a compound which is a polysaccharide derivative of EPO, or of an EPO like protein, wherein the polysaccharide is anionic and comprises between 2 and 200 saccharide units. The present invention also relates to pharmaceutical compositions comprising the novel compounds, and methods for making the novel compounds.

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Номер записи: 79

09-05-2013 дата публикации

Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same

Номер: US20130117892A1

Автор: Brown Kimberly, Harris Paul, Maiyuran Suchindra

Принадлежит: NOVOZYMES, INC.

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having cellulolytic enhancing activity , selected from the group consisting of:(a) a polypeptide comprising an amino acid sequence having at least 60% identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1 or SEQ ID NO: 3, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO: 1 or SEQ ID NO: 3, or (iii) a full-length complementary strand of (i) or (ii);(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% identity to the mature polypeptide coding sequence of SEQ ID NO: 1; and(d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.2. The polypeptide of claim 1 , comprising or consisting of the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4; or a fragment thereof having cellulolytic enhancing activity.3E. coliE. coli. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid plasmid pSMai190 which is contained in NRRL B-50083 or plasmid pSMai192 which is contained in NRRL B-50085.4. An isolated polynucleotide comprising a nucleotide sequence that encodes the polypeptide .5. A nucleic acid construct comprising the polynucleotide of operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.6. A recombinant host cell comprising the nucleic acid construct of .7. A method of ...

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Номер записи: 80

16-05-2013 дата публикации

GLYCEROGLYCOLIPID LIPASE

Номер: US20130122144A1

Автор: FUKAZAWA TETSUYA, TANAKA ISSHIN, TSUKAZAKI KAORU

Принадлежит:

Disclosed is a glyceroglycolipid lipase which is highly safe, can hydrolyze a neutral fat, a glycerophospholipid or a glyceroglycolipid at about pH 6, is thermally stable to some extent, can hydrolyze lecithin, cannot hydrolyze lysolecithin, can rise a bread when used singly in the production of the bread, and has no unpleasant odor. Specifically disclosed is a glyceroglycolipid lipase derived from a filamentous bacterium 1. A glyceroglycolipid lipase having the following properties:1) having a molecular weight of approximately 29,000 as determined by SDS-PAGE;2) hydrolyzing neutral fat, lecithin, and glyceroglycolipid at pH 6.0;3) having a glyceroglycolipid degradation activity at least 10-fold higher than a lecithin degradation activity at pH ranging from 3.6 to 8.9.2. The glyceroglycolipid lipase according to claim 1 , which further has the following properties:4) having a relative degradation activity of at least 80% or more for glyceroglycolipid at pH ranging from 4.1 to 7.7;5) having a relative degradation activity of at least 80% or more for lecithin at pH ranging from 5.1 to 7.1;6) not having the hydrolytic activity of 5) at a temperature of 80° C. or higher;7) having a relative residual degradation activity of 75% or more for glyceroglycolipid at pH ranging from 4.1 to 10.7.3Aspergillus japonicus.. The glyceroglycolipid lipase according to or claim 1 , which is derived from a filamentous fungus4Aspergillus japonicus. The glyceroglycolipid lipase according to claim 3 , wherein the filamentous fungus is strain SANK 11298.5. A glyceroglycolipid lipase which is a protein of any one of the following a) to e):a) a protein consisting of the amino acid sequence of SEQ ID NO: 2 of the sequence listing;b) a protein consisting of an amino acid sequence encoded by the nucleotide sequence from nucleotide number 110 to nucleotide number 991 of SEQ ID NO: 1 of the sequence listing;c) a protein consisting of an amino acid sequence in which one or several amino acids are ...

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Номер записи: 81

16-05-2013 дата публикации

Eubacterial RNA-Polymerase Mutants With Altered Product Production

Номер: US20130122548A1

Автор: Andersen Jens Toenne, Banke Niels, Joergensen Steen Troels, Nielsen Preben

Принадлежит: NOVOZYMES A/S

The present invention relates to an isolated mutant comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant and to a use of the mutant according to the invention for producing at least one product of interest. 1eubacterium. An isolated mutant comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in the isogenic parent strain grown at identical conditions , wherein the substitution of at least one amino acid occurs at any of positions 469 , 478 , 482 , 485 , or 487 in SEQ ID NO:2 , or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member.2eubacterium. The isolated mutant of claim 1 , wherein the substitution of at least one amino acid provides an improved production of the product of interest claim 1 , or a higher yield of the product of interest.3eubacterium. The isolated mutant of claim 1 , wherein the substitution of at least one amino acid comprises Q469R claim 1 , A478D claim 1 , A478V claim 1 , H482R claim 1 , H482P claim 1 , R485H claim 1 , or S487L.4eubacterium. The isolated mutant of claim 1 , wherein the substitution of at least one ...

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Номер записи: 82

16-05-2013 дата публикации

High Fidelity Restriction Endonucleases

Номер: US20130122568A1

Автор: Chan Siu-hong, Guan Shengxi, Quimby Aine, Sun Dapeng, Wei Hua, Xu Shuang-Yong, Zhang Penghua, Zhu Zhenyu

Принадлежит: NEW ENGLAND BIOLABS, INC.

Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. 1. A composition , comprising: a restriction endonuclease having at least one artificially introduced mutation and an overall fidelity index (FI) improvement factor of at least 2 , the restriction endonuclease being capable of cleaving a substrate with at least a similar cleavage activity to that of the restriction endonuclease absent the artificially introduced mutation , in a predetermined buffer , wherein the artificially introduced mutation is the product of at least one of a targeted mutation , saturation mutagenesis , or a mutation introduced through a PCR amplification procedure.2. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with an oppositely charged residue at a target site in the restriction endonuclease.3. A composition claim 1 , according to claim 1 , wherein at least one of the artificially introduced mutations is a replacement of a naturally occurring residue with a residue selected from a Phenylalanine and an Alanine at a target site in the restriction endonuclease.4. A composition claim 1 , according to claim 1 , wherein the restriction enzyme absent the at least one artificially introduced mutation is selected from the group consisting of: BamHI claim 1 , EcoRI claim 1 , Scal claim 1 , SalI claim 1 , SphI claim 1 , PstI claim 1 , NcoI claim 1 , NheI claim 1 , SspI claim 1 , NotI claim 1 , SacI claim 1 , PvuII claim 1 , MfeI claim 1 , HindIII claim 1 , SbfI claim 1 , EagI claim ...

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Номер записи: 83

16-05-2013 дата публикации

Expression of Granular Starch Hydrolyzing Enzymes in Trichoderma and Process for Producing Glucose from Granular Starch Sustrates

Номер: US20130122569A1

Автор: Baldwin Toby M., Bower Benjamin S., Chotani Gopal K., Dunn-Coleman Nigel, JR. Oreste J., Lantero, Lantz Suzanne, Pepsin Michael J., Shetty Jayarama K., Strohm Bruce A.

Принадлежит: DANISCO US INC.

The present invention relates to filamentous fungal host cells and particularly host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup. 1Trichoderma reeseiTrichoderma reesei. A fermentation medium comprising a granular starch hydrolyzing enzyme having glucoamylase activity (GSHE) produced from a culture of , wherein the comprises a heterologous polynucleotide encoding a GSHE having at least 90% amino acid sequence identity with SEQ ID NO: 3 or with SEQ ID NO: 6.218-. (canceled) The present application is a divisional application of U.S. patent application Ser. No. 10/991,654, filed Nov. 18, 2004, and claims priority to U.S. Provisional Patent Application Ser. No. 60/524,279 entitled Expression of Granular Starch Hydrolyzing Enzyme in , filed Nov. 21, 2003; U.S. Provisional Patent Application Ser. No. 60/531,953 entitled Enzyme Compositions for Glucose Feed from Granular Starch Substrates, filed Dec. 22, 2003; and U.S. Provisional Patent Application Ser. No. 60/566,358 entitled Expression of Granular Starch Hydrolyzing Enzyme in , filed Apr. 28, 2004.The present invention relates to filamentous fungal host cells useful for the expression of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). The invention further relates to the use of the GSHE in methods for producing glucose syrup and other end products from granular starch substrates comprising contacting a granular starch substrate, at or below the gelatinization temperature of the granular starch, simultaneously with a starch liquefying amylase and a GSHE. The invention ...

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Номер записи: 84

16-05-2013 дата публикации

Glucoamylase Variants

Номер: US20130122570A1

Автор: Svendsen Allan

Принадлежит: NOVOZYMES A/S

The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates. 1. A variant of a parent glucoamylase comprising one or more mutation(s) in position(s) or region(s) corresponding to the following position(s) or region(s) in the amino acid sequence of SEQ ID NO: 2:a) said variant comprising one or more insertion(s) in: Region: 1-35, Region: 40-58, Region: 60-62, Region: 73-80, Position: 93, Region: 95-101, Region: 103-121, Region: 123-124, Region: 126-127, Region: 170-175, Region: 177-184, Region: 200-212, Region: 234-246, Region: 287-312, Region: 314-319, Region: 334-339, Position: 341, Region: 354-356, Position: 358, Region: 360-374, Region: 388-392, Position: 394, Region: 396-401, Region: 403-407, Region: 409-414, Region: 445-449, Region: 452-467, Region: 469-470, and/or in a corresponding position or region in a homologous glucoamylase which displays at least 60% homology with the amino acid sequences shown in SEQ ID NO: 2, orb) said variant comprising one or more substitution(s), insertion(s) and/or deletion(s) in: Region: 36-39, Region: 63-65, Position 67, Region: 69-71, Region: 81-92, Region: 128-169, Region: 185-188, Region: 190-199, Region: 213-222, Region: 224-226, Region: 228-233, Region: 247-271, Region: 273-286, Region: 320-333, Region: 343-344, Region: 346-347, Region: 349-351, Region: 375-378, Region: 380-385, Position: 387, Position: 415, Region: 417-424, Position: 426, Region: 428-443, Region: 471-485, Region: 487-489, Region: 491-493, Region: 495-616, and/or in a corresponding position or region in a homologous glucoamylase which displays at least 60% homology with the amino acid sequence shown in SEQ ID NO: 2, except the following amino acid substitutions: A39V, P128S, G137A, G139A, D153N, S185H, G251A, D257E, E259D, E259Q, C320A, D375C, G383A, W417F, S431C, A435P, S436P, W437F, A442T, A471C, A479C, T480C, P481C, A495T and A495P.222-. ( ...

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Номер записи: 85

16-05-2013 дата публикации

Tal effector-mediated dna modification

Номер: US20130122581A1

Автор: Adam J. Bogdanove, Daniel F. Voytas, Feng Zhang

Принадлежит: Iowa State University Research Foundation ISURF, University of Minnesota

Materials and Methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; “TALENS”) are provided.

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Номер записи: 86

16-05-2013 дата публикации

Polypeptides Having Lipase Activity And Polynucleotides Encoding Same

Номер: US20130123163A1

Автор: Amolo Christopher, Borch Kim, Cherry Barbara, Ge Haiyan, Lamsa Michael, Lin Janine, Otani Suzanne, Patkar Shamkant Anant, Yaver Debbie

Принадлежит:

The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. 1. An isolated polypeptide having lipase activity , selected from the group consisting of:(a) a polypeptide comprising an amino acid sequence having at least 90% identity with the mature polypeptide of SEQ ID NO: 8 or SEQ ID NO: 10;(b) a polypeptide encoded by a polynucleotide which hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 7 or SEQ ID NO: 9, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO: 7 or SEQ ID NO: 9, or (iii) a full-length complementary strand of (i) or (ii), wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 90% identity with the mature polypeptide coding sequence of SEQ ID NO: 7 or SEQ ID NO: 9.2. The polypeptide of claim 1 , which comprises the amino acid sequence of SEQ ID NO: 8 or SEQ ID NO: 10; or a fragment thereof having lipase activity.3. The polypeptide of claim 1 , which comprises the mature polypeptide of SEQ ID NO: 8 or SEQ ID NO: 10.4. The polypeptide of claim 1 , which consists of SEQ ID NO: 8 or SEQ ID NO: 10; or a fragment thereof having lipase activity.5. The polypeptide of claim 1 , which consists of the mature polypeptide of SEQ ID NO: 8 or SEQ ID NO: 10.6E. coliE. coli. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pCrAm138 which is contained in NRRL B-30781 claim 1 , or ...

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Номер записи: 87

23-05-2013 дата публикации

Method of producing recombinant proteins with mannose-terminated n-glycans

Номер: US20130129755A1

Автор: Zhiwei Song

Принадлежит: Agency for Science Technology and Research Singapore

We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-1 into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease.

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Номер записи: 88

23-05-2013 дата публикации

Endoribonuclease compositions and methods of use thereof

Номер: US20130130248A1

Автор: Blake Wiedenheft, Jennifer A. DOUDNA, Martin Jinek, Rachel E. Haurwitz

Принадлежит: UNIVERSITY OF CALIFORNIA

The present disclosure provides variant Csy4 endoribonucleases, nucleic acids encoding the variant Csy4 endoribonucleases, and host cells genetically modified with the nucleic acids. The variant Csy4 endoribonucleases find use in a variety of applications, which are also provided. The present disclosure also provides methods of detecting a specific sequence in a target polyribonucleotide; and methods of regulating production of a target RNA in a eukaryotic cell.

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Номер записи: 89

23-05-2013 дата публикации

Method for Reducing Star Activity in Restriction Endonucleases

Номер: US20130130293A1

Автор: Matheshwaran Saravanan, Valakunja Nagaraja, Zhenyu Zhu

Принадлежит: New England Biolabs Inc

Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.

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Номер записи: 90

23-05-2013 дата публикации

Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase and Beta-Xylosidase Activity and Polynucleotides Encoding Same

Номер: US20130130327A1

Автор: Morant Marc Dominique

Принадлежит:

The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having beta-glucosidase activity , beta-xylosidase activity , or beta-glucosidase and beta-xylosidase activity , selected from the group consisting of:(a) a polypeptide having at least 70% sequence identity to the mature polypeptide of SEQ ID NO: 2, or the mature polypeptide SEQ ID NO: 4;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least midium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 3 or the cDNA sequence thereof,(c) a polypeptide encoded by a polynucleotide having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 3 or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2, or the mature polypeptide SEQ ID NO: 4, comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity.2. An isolated polynucleotide encoding the polypeptide of .3. A recombinant host cell comprising the polynucleotide of operably linked to one or more control sequences that direct the production of the polypeptide.4. A method of producing the polypeptide of claim 1 , comprising:(a) cultivating a cell, which in its wild-type form produces the polypeptide, under conditions conducive for ...

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Номер записи: 91

23-05-2013 дата публикации

NOVEL GLYCOSYL HYDROLASE WITH BETA-XYLOSIDASE AND BETA-GLUCOSIDASE ACTIVITIES AND USES THEREOF

Номер: US20130130330A1

Автор: Cheng Haili, Cheng Kedi, He Huixia, Meng Chao, Zhao Ruiyu, Zhu Huixin, Zhu Ping

Принадлежит: INSTITUTE OF MATERIA MEDICA, CHINESE ACADEMY OF ME SCIENCES

A novel glycosyl hydrolase with activities of beta-xylosidase and beta-glucosidase is provided. Said glycosyl hydrolase can convert 7-xylosyltaxane compounds to 7-hydroxyltaxane compounds. 1. A glycosyl hydrolase , characterized in that the amino acid sequence of the glycosyl hydrolase comprises ,an amino acid sequence that has at least 50% identity with SEQ ID NO: 2.2. The glycosyl hydrolase according to claim 1 , characterized in that the amino acid sequence of the glycosyl hydrolase comprises claim 1 ,an amino acid sequence that has at least 60% identity with SEQ ID NO: 2.3. The glycosyl hydrolase according to claim 2 , characterized in that the amino acid sequence of the glycosyl hydrolase comprises claim 2 ,an amino acid sequence that has at least 70% identity with SEQ ID NO: 2.4. The glycosyl hydrolase according to claim 3 , characterized in that the amino acid sequence of the glycosyl hydrolase comprises claim 3 ,an amino acid sequence that has at least 80% identity with SEQ ID NO: 2.5. The glycosyl hydrolase according to claim 4 , characterized in that the amino acid sequence of the glycosyl hydrolase comprises claim 4 ,an amino acid sequence that has at least 90% identity with SEQ ID NO: 2.6. The glycosyl hydrolase according to claim 5 , characterized in that the amino acid sequence of the glycosyl hydrolase comprises claim 5 ,an amino acid sequence that has at least 95% identity with SEQ ID NO: 2.7. The glycosyl hydrolase according to claim 6 , characterized in that the amino acid sequence of the glycosyl hydrolase is a protein which is derived from SEQ ID NO: 2 by substitution claim 6 , deletion or addition of one or several amino acid residues in the amino acid sequence of SEQ ID NO: 2 and which has the same activity as the amino acid residue sequence of SEQ ID NO: 2.8. A nucleotide sequence encoding the glycosyl hydrolase according to .9. The nucleotide sequence according to claim 8 , which is characterized in that the nucleotide sequence comprises ...

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Номер записи: 92

23-05-2013 дата публикации

Variants of Glycoside Hydrolases

Номер: US20130130353A1

Автор: Cherry Joel, Harris Paul, Jones Aubrey, Teter Sarah, Ward Connie, Yi Jung

Принадлежит: NOVOZYMES, INC.

The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences. 1. An isolated variant of a parent glycoside hydrolase , comprising a substitution at a position corresponding to position 373 of SEQ ID NO: 2 , wherein the variant comprises an amino acid sequence that has at least 90% sequence identity to SEQ ID NO: 2 , and wherein the variant has glycoside hydrolase activity.2. The variant of claim 1 , which comprises an amino acid sequence that has at least 95% sequence identity to SEQ ID NO: 2.3. The variant of claim 1 , which comprises an amino acid sequence that has at least 97% sequence identity to SEQ ID NO: 2.4. The variant of claim 1 , wherein the parent glycoside hydrolase comprises SEQ ID NO: 2 claim 1 , or a fragment thereof that has glycoside hydrolase activity.5. The variant of claim 1 , wherein the parent glycoside hydrolase comprises SEQ ID NO: 2.6. The variant of claim 1 , wherein the substitution is His.7. The variant of claim 1 , wherein the substitution is N373H.8. The variant of claim 1 , further comprising a substitution at a position corresponding to position 21 claim 1 , 94 ...

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Номер записи: 93

30-05-2013 дата публикации

Angiogenin and Variants Thereof for Treatment of Neurodegenerative Diseases

Номер: US20130136727A1

Автор: Guo-Fu Hu, Hiroko Kishikawa

Принадлежит: Harvard College

Methods and compositions for treating neurodegenerative disorders using angiogenin and/or angiogenin variants are provided.

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Номер записи: 94

30-05-2013 дата публикации

Methods and materials for reducing biofilms

Номер: US20130136730A1

Автор: Kristi L. Frank, Robin Patel

Принадлежит: Mayo Foundation for Medical Education and Research

This document provides methods and materials related to reducing biofilms. For example, enzymes (e.g., glycosyl hydrolases), nucleic acid molecules encoding enzymes, host cells containing nucleic acid encoding enzymes, and methods for using enzymes to reduce biofilms and infections associated with biofilms are provided.

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Номер записи: 95

30-05-2013 дата публикации

Use of a beta-glucosidase activator for the detection and/or identification of c. difficile

Номер: US20130137126A1

Автор: Diane Halimi, Marie Cellier, Sylvain Orenga

Принадлежит: bioMerieux SA

The present invention relates to a reaction medium comprising at least one beta-glucosidase substrate and a compound of the general formula Ar-beta-D-glucoside where Ar- designates an aromatic compound, different from said substrate. According to the invention, such a medium can be employed in a C. difficile detection and/or identification process.

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Номер записи: 96

30-05-2013 дата публикации

Expression of Beta-Mannanase in Chloroplasts and its Utilization in Lignocellulosic Woody Biomass Hydrolysis

Номер: US20130137142A1

Автор: Daniell Henry

Принадлежит: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol. In other exemplary embodiments, methods and materials are disclosed for degrading mannans. 1. A method of degrading a plant biomass sample so as to release fermentable sugars therein , the method comprising admixing with said biomass sample a plant degrading cocktail comprising at least one cell extract , said cell extract comprising an active plant degrading compound recombinantly expressed in cells from which said cell extract is derived , said at least one cell extract comprising β-Mannanase.2. The method of claim 1 , further comprising at least two cell extracts claim 1 , wherein said at least two cell extracts comprise plant cell extract comprising a first plant degrading compound claim 1 , β-Mannanase claim 1 , and at least one other cell extract comprising a second plant degrading compound.3. The method of claim 2 , wherein said at least two cell extracts are provided in a plant degrading cocktail claim 2 , and said second plant degrading compound comprises cellulase claim 2 , ligninase claim 2 , beta-glucosidase claim 2 , hemicellulase claim 2 , xylanase claim 2 , alpha amylase claim 2 , amyloglucosidase claim 2 , pectate lyase claim 2 , cutinase claim 2 , lipase claim 2 , maltogenic alpha-amylase claim 2 , pectolyase or expansin.4. The method of claim 1 , wherein said plant biomass sample comprises grain and/or grain residues claim 1 , sugar beet claim 1 , sugar cane claim 1 , grasses claim 1 , wood-based biomass claim 1 , fruits and/or fruit waste residues claim 1 , or a combination thereof.5. The method ...

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Номер записи: 97

30-05-2013 дата публикации

Polypeptides Having Glucoamylase Activitiy and Polynucleotides Encoding Same

Номер: US20130137152A1

Автор: Ayabe Keiichi, Coward-Kelly Guillermo, Landvik Sara

Принадлежит:

The present invention relates to isolated polypeptides having glucoamylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains. 128-. (canceled)29. An isolated polypeptide having glucoamylase activity , selected from the group consisting of:(a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2; and(b) a fragment of SEQ ID NO: 2 that has glucoamylase activity.30. The polypeptide of claim 29 , having at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2.31. The polypeptide of claim 29 , having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2.32. The polypeptide of claim 29 , having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.33. The polypeptide of claim 29 , comprising or consisting of SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2.34. The polypeptide of claim 33 , wherein the mature polypeptide is amino acids 18 to 568 of SEQ ID NO: 2.35. The polypeptide of claim 29 , which is encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1 claim 29 , (ii) the cDNA sequence thereof claim 29 , or (iii) the full-length complement of (i) or (ii).36. The polypeptide of claim 29 , which is a fragment of SEQ ID NO: 2 that has glucoamylase activity.37. An isolated polypeptide comprising a catalytic domain selected from the group consisting of:(a) a catalytic domain having at least 80% sequence identity to amino acids 19 to 468 of SEQ ID NO: 2; and(b) a fragment of amino acids 19 to 468 of SEQ ID NO: 2 that has glucoamylase activity.38. The polypeptide of claim ...

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Номер записи: 98

30-05-2013 дата публикации

LIPASE-CONTAINING POLYMERIC COATINGS FOR THE FACILITATED REMOVAL OF FINGERPRINTS

Номер: US20130137159A1

Автор: Buthe Andreas, Ishii Masahiko, Jia Hongfei, Wang Ping, Wu Songtao, Zhang Minjuan

Принадлежит:

A substrate or coating is provided that includes a lipase with enzymatic activity toward a component of a fingerprint. Also provided is a process for facilitating the removal of fingerprints is provided wherein an inventive substrate or coating including a lipase is capable of enzymatically degrading of one or more components of the fingerprint to facilitate fingerprint removal from the substrate or said coating. Applying heat to the substrate or coating increases the rate of fingerprint removal. 1. A composition for facilitating fingerprint removal comprising:a coating material; anda lipase capable of degrading a fingerprint component, said lipase associated with said coating material so as to be capable of facilitating fingerprint removal when said coating material is contacted by a fingerprint.2. The composition of wherein said coating comprises an organic crosslinkable polymer resin having a functional group of acetoacetate claim 1 , acid claim 1 , amine claim 1 , carboxyl claim 1 , epoxy claim 1 , hydroxyl claim 1 , isocyanate claim 1 , silane claim 1 , vinyl claim 1 , or combinations thereof.3. The composition of wherein said organic crosslinkable polymer resin is aminoplasts claim 2 , melamine formaldehydes claim 2 , carbamates claim 2 , polyurethanes claim 2 , polyacrylates claim 2 , epoxies claim 2 , polycarbonates claim 2 , alkyds claim 2 , vinyls claim 2 , polyamides claim 2 , polyolefins claim 2 , phenolic resins claim 2 , polyesters claim 2 , polysiloxanes claim 2 , or combinations thereof.4. The composition of wherein said organic crosslinkable polymer is a hydroxyl-functionalized acrylate resin.5. The composition of wherein said lipase is lipoprotein lipase claim 1 , acylglycerol lipase claim 1 , hormone-sensitive lipase claim 1 , phospholipase A1 claim 1 , phospholipase A2 claim 1 , phospholipase C claim 1 , phospholipase D claim 1 , phosphoinositide phospholipase C claim 1 , a lysophospholipase claim 1 , or a galactolipase.6. The composition of ...

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Номер записи: 99

30-05-2013 дата публикации

Nucleotide-Specific Recognition Sequences For Designer TAL Effectors

Номер: US20130137160A1

Автор: Cong Le, Zhang Feng

Принадлежит:

The invention relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in an animal cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that includes a deoxyribonucleic acid (DNA) binding polypeptide having a N-terminal capping region, a DNA binding domain comprising at least five or more Transcription activator-like effector (TALE) monomers and at least one or more half-monomers specifically ordered to target the genomic locus of interest, and a C-terminal capping region, wherein the polypeptide includes at least one or more effector domains, and wherein the polypeptide is encoded by and translated from a codon optimized nucleic acid molecule so that the polypeptide preferentially binds to the DNA of the genomic locus. 117-. (canceled)18. A non-naturally occurring or engineered composition comprising a deoxyribonucleic acid (DNA) binding polypeptide comprising:(a) a N-terminal capping region(b) a DNA binding domain comprising at least five or more Transcription activator-like effector (TALE) monomers and at least one or more half-monomers specifically ordered to target a genomic locus of interest, and(c) a C-terminal capping regionwherein (a), (b) and (c) are arranged in a predetermined N-terminus to C-terminus orientation,wherein the polypeptide includes at least one or more effector domains,wherein the polypeptide is encoded by and translated from a codon optimized nucleic acid molecule so that the polypeptide preferentially binds to DNA of the genomic locus,{'sub': 1-11', '12', '13', '14-33 or 34 or 35', 'z, 'wherein the DNA binding domain comprises (X-XX-X),'}{'sub': '1-11', 'wherein Xis a chain of 11 contiguous amino acids,'}{'sub': 12', '13, 'wherein XXis a repeat variable diresidue (RVD),'}{'sub': '14-33 or 34 or 35', 'wherein Xis a chain of 21, 22 or 23 contiguous amino acids,'}wherein z is at least 5 to 40, andwherein at least one ...

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Номер записи: 100