БЕТА-ГЛЮКАНАЗЫ TALAROMYCES EMERSONII

Изобретение относится к биотехнологии и представляет собой бета-глюканазу, получаемую из гриба Talaromyces emersonii, или композицию, обладающую активностью бета-глюканазы. Данный фермент или композиция используются при обработке растительного материала для разрушения или модификации целлюлозы, а также входят в состав пищевых продуктов и кормов для животных. Заявленное изобретение позволяет расширить ассортимент ферментов, обладающих бета-глюканазной активностью. 9 н. и 8 з.п. ф-лы, 15 табл.

РАЗРУШАЮЩИЕСЯ ОБЕРТКИ ФИЛЬТРОВ И ВАРИАНТЫ ИХ ПРИМЕНЕНИЯ

Изобретение относится к фильтрующему стержню для курительного изделия, который содержит стержень фильтрующего материала и обертку заглушки, окружающую стержень фильтрующего материала, с перекрывающимися боковыми краями обертки заглушки, скрепленными вместе адгезивом обертки заглушки, содержащим агент, ускоряющий разложение, причем агент, ускоряющий разложение, включает фермент. Технический результат заключается в обеспечении повышенной скорости разложения. 2 н. и 11 з.п. ф-лы, 3 ил.

СТАБИЛЬНОЕ ПРИ ХРАНЕНИИ ЖИДКОЕ МОЮЩЕЕ ИЛИ ЧИСТЯЩЕЕ СРЕДСТВО, СОДЕРЖАЩЕЕ ПРОТЕАЗУ И ЦЕЛЛЮЛАЗУ

Изобретение относится к области биохимии. Представлено применение модифицированной протеазы в качестве средства для повышения стабильности при хранении целлюлазы в жидком моющем или чистящем средстве, включающем целлюлазу и протеазу. Изобретение обеспечивает пониженную дезактивацию целлюлазы посредством вышеуказанной протеазы, тем самым позволяя сохранить высокую целлюлолитическую остаточную активность в указанном моющем или чистящем средстве даже после 8 недель хранения. 7 з.п. ф-лы, 2 табл., 1 пр.

УЛУЧШЕННЫЙ СПОСОБ ПРОЛУЧЕНИЯ ФЕРМЕНТОВ ЦЕЛЛЮЛАЗЫ И/ИЛИ ГЕМИЦЕЛЛЮЛАЗЫ

... 1. Способ получения ферментов целлюлазы и/или гемицеллюлазы микроорганизмом, включающий по меньшей мере одну стадию роста в присутствии углеродного источника и по меньшей мере одну стадию продуцирования в присутствии индуцирующего субстрата, в котором указанный индуцирующий субстрат представляет собой смесь глюкозы или целлюлозных гидролизатов, лактозы и ксилозы или раствора гемицеллюлолитических гидролизатов, причем содержание каждого из компонентов смеси определено в следующих границах, мас.%:- от 40 до 65 глюкозы или целлюлозных гидролизатов,- от 21 до 25 лактозы и- от 10 до 39 ксилозы или раствора гемицеллюлозных гидролизатов,причем суммарное количество этих трех компонентов равно 100%.2. Способ по п.1, в котором микроорганизм относится к виду Trichoderma reesei и делетируется для катаболитной репрессии глюкозой.3. Способ по п.1 или 2, в котором внесение индуцирующего субстрата осуществляется в растворе, причем концентрация индуцирующего субстрата в питательном растворе, используемом ...

НЕБОЛЬШИЕ ФЕРМЕНТО-СОДЕРЖАЩИЕ ГРАНУЛЫ

... 1. Совокупность ферменто-содержащих гранул, где, по меньшей мере, около 95% указанных гранул содержит одно ядро и ферменто-содержащий слой, нанесенный поверх указанного ядра, причем указанное ядро состоит из одной или нескольких неорганических солей, и где, по меньшей мере, около 80% указанных гранул имеет диаметр около 150-355 мкм. ! 2. Совокупность гранул по п. 1, где указанное ядро состоит из сульфата натрия. ! 3. Совокупность гранул по п. 2, где, по меньшей мере, около 80% указанных натриево-сульфатных ядер имеет диаметр около 100-250 мкм. ! 4. Совокупность гранул по п. 1, где ферменто-содержащий слой дополнительно содержит, по меньшей мере, один из компонентов: полимер, сахар, крахмал или поверхностно-активное вещество. ! 5. Совокупность гранул по п. 1, дополнительно содержащая нанесенный поверх указанного ферменто-содержащего слоя слой, содержащий барьерную соль. ! 6. Совокупность гранул по п. 5, где барьерный солевой слой содержит сульфат натрия. ! 7. Совокупность гранул по п. 5, ...

СИСТЕМЫ И СПОСОБЫ ДЛЯ ИЗМЕНЕНИЯ СКОРОСТИ ЭНЗИМАТИЧЕСКИХ ПРОЦЕССОВ

... 1. Способ увеличения скорости гидролиза полисахарида, включающий: ! обеспечение полисахарида в водной среде; ! смешивание фермента и полимера с водной средой для сформирования по существу гомогенной смеси фермента, полимера и полисахарида в водной среде; и вступление в реакцию фермента с полисахаридом в присутствии полимера для преобразования, по крайней мере, части полисахарида в глюкозу, где скорость реакции фермента с полисахаридом в присутствии полимера больше, чем скорость реакции фермента с полисахаридом в отсутствии полимера. ! 2. Способ по п.1, где полисахарид включает целлюлозу или ее производную, и где фермент включает целлюлазу. ! 3. Способ по п.1, где полисахарид включает крахмал или его производную, и где фермент включает амилазу. ! 4. Способ по п.1, где полимер включает катионный полимер. ! 5. Способ по п.4, где полимер имеет молекулярную массу приблизительно от 100000 Да до приблизительно 20 миллионов Да и плотность заряда от приблизительно 5% до приблизительно 95%. ! 6.

ЦЕЛЬНОКЛЕТОЧНЫЕ БИОКАТАЛИЗАТОРЫ В ДЕГРАДАЦИИ ЦЕЛЛЮЛОЗНОЙ БИОМАССЫ

... 1. Молекула нуклеиновой кислоты, содержащая следующие компоненты:(1) участок, кодирующий сигнальный пептид,(2) участок, кодирующий гетерологичную целлюлазу,(3) необязательный участок, кодирующий сайт распознаваемый протеазой,(4) участок, кодирующий трансмембранный линкер, и(5) участок, кодирующий транспортный домен белка-автотранспортера или его вариант.2. Молекула нуклеиновой кислоты по п. 1, отличающаяся тем, что целюлаза является бета-глюкозидазой или эндо- либо экзоцеллюлазой, предпочтительно выбираемой из группы, содержащей эндоцеллюлазу Bacillus subtilis, экзоцеллюлазу Clostridium thermocellum и бета-глюкозидазу Clostridium thermocellum.3. Молекула нуклеиновой кислоты по п. 1, отличающаяся тем, что транспортный домен белка-автотранспортера выбирают из группы, содержащей Ssp, Ssp-h1, Ssp-h2, PspA, PspB, Ssa1, SphB1, AspA/NalP, VacA, AIDA-I, IcsA, MisL, TibA, Ag43, ShdA, AutA, Tsh, SepA, EspC, EspP, Pet, Pic, SigA, Sat, Vat, EpeA, EatA, EspI, EaaA, EaaC, Pertactin, BrkA, Tef, Vag8, ...

ENDO-beta-1,4-GLUKANASE UND IHRE ENTSPRECHENDE DNA

MICROORGANISMS, ESPECIALLY E. COLI, TRANSFORMED WITH A DNA SEQUENCE ORIGINATING FROM C. THERMOCELLUM, COMPOSITIONS HAVING A CELLULOLYTIC ACTIVITY ISSUED FROM THESE MICROORGANISMS AND PROCESS TO OBTAIN THEM

CELLULASEZUSAMMENSETZUNGEN MIT EINEM DEFIZIT AN KOMPONENTEN DES TYPS-CBH I ENTHALTENDE REINIGUNGSMITTELZUSAMMENSETZUNGEN

MUTIERTE ALKALISCHE CELLULASE

ENDOGLUCANASE-ENZYM UND CELLULASE-ZUSAMMENSETZUNGEN, DIE DIESES ENTHALTEN

MUTIERTE TRICHODERMA REESEI EGIII CELLULASEN, DAFÜR KODIERENDE DNA UND VERFAHREN ZU DEREN HERSTELLUNG

Enzyme

Method of removing seed coats of soybeans and defatted soybeans with cellulase produced by microorganisms

Cellulase, produced by propagating Trichoderma, Myrothrecium Penicillium, Aspergillus or Actinomycetes microorganisms, particularly Trichoderma viridae, on steam-sterilized wet bran or an aqueous culture medium, is used, in the form of an aqueous solution having a pH between 3.5 and 6.5 for removing seed coats from soyabeans (see Division A2). The aqueous solution of cellulase may have a potency of 40 units per ml., and may be the aqueous cultured medium itself, a filtrate thereof, an aqueous extract from said cultured bran, or a solution of dry, refined cellulose (precipitated from the extract and having a potency of 4000 units per gm.) in an aqueous sodium acetate-acetic acid buffer mixture. Pectinase may be added to the said aqueous solution.

PROCESS FOR PREPARATION OF ENZYMES

... 1465307 Drain cleaning composition AMANO PHARMACEUTICAL CO Ltd 1 Nov 1974 [10 Nov 1973] 47325/74 Heading C5D [Also in Divisions C1 and C3] The novel enzyme cellulase 212 whose activity in the alkaline region within a broad pH range of from 4À5 to 9À0 renders it very effective as an ingredient in cleaning agents, is prepared by aerobically culturing the strain Aeromonas sp. No. 212 (FERM-P No. 2306 and ATCC No. 31085) in a culture medium maintained at a pH of 5-10 and a temperature of 27-45‹ C. for 1-5 days and recovering the enzyme by conventional procedures of adsorption and gel filtration. Example 8 discloses a cleaning agent useful for cleaning drains clogged with cellulosic material and consisting of 50 parts by weight crude powdered cellulase 212, 10 parts primary sodium phosphate, 35 parts secondary sodium phosphate and 5 parts of polyoxyethylene glycol.

Method for treating cellulosic material and CGHII/CEL4A enzymes useful therein

Processing biomass

Talaromyces emersonii enzyme system

Treatment of cellulosic material and enzymes useful therein

Improved endoglucanases for treatment of cellulosic material

METHOD FOR TREATING CELLULOSIC MATERIAL AND CBHII/CEL6A ENZYMES USEFUL THEREIN

Talaromyces emersonii enzyme system

Treatment of cellulosic material and enzymes useful therein

Treatment of cellulosic material and enzymes useful therein

Novel proteins for the treatment of cellulosic material

Processing of biomass materials

Processing biomass

Talaromyces emersonii enzyme system

Method for treating cellulosic material and CGHII/CEL4A enzymes useful therein

Treatment of cellulosic material and enzymes useful therein

Improved endoglucanases for treatment of cellulosic material

Processing biomass

Processing of biomass materials

Method for treating cellulosic material and CGHII/CEL4A enzymes useful therein

Processing of biomass materials

Improved endoglucanases for treatment of cellulosic material

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

SYSTEMS TO THE MASS PRODUCTION OF PROTEINS OR PEPTIDEN BY MICROORGANISMS OF THE KIND HUMICOLA

IMPROVED CELLULASE CONNECTION TO THE TREATMENT OF ZELLULOSEBEHALTIGEN TEXTILES

CELLULOLYTI ENZYME EXPRIMIERENDE ONE TRANSGENE PLANTS

CELLULASEVARIANTEN

PROCEDURE FOR THE PRODUCTION OF GLUCOSE ALSO A ERMODIFIZIERTEN ZELLULASE

PROCEDURE FOR THE PRODUCTION OF CELLULASE AND PLANT FOR THE EXECUTION OF THE PROCEDURE

POLYPEPTIDE WITH CELLO BIO HYDROLASE II-ACTIVITY AND BUT CODING POLYNUCLEOTIDE

NICHTIONI TENSID ABSTENTION CELLULASEPRÄPARATION AND PROCEDURES FOR THE FIBER TREATMENT

CELLULASE THERMAL STABILITY IMPROVED BY T. REESEI WITH

WITHOUT PRESSURE TAKING PLACE PRETREATMENT, ENZYMATIC HYDROLYSIS AND FERMENTATION OF WASTE PARLIAMENTARY GROUPS

PROCEDURE FOR THE PRODUCTION OF CELLULASE AND PLANT FOR THE EXECUTION OF THE PROCEDURE

POLYPEPTIDE WITH ENDOGLUCANASE AKTIVTÄT AND BUT CODING POLYNUKLEOTIDE

POLYPEPTIDE WITH ENDOGLUCANASE ACTIVITY AND POLYNUKLEOTIDE TO YOUR CODING

TRANSFORMATION OF TRICHODERMA.

A PROCEDURE FOR THE STOFFAUFBEREITUNG USING CELLULASE.

PROTEINS WITH ZELLULASE ACTIVITIES AND PROCESS FOR YOUR PRODUCTION

ZELLULASE ENZYMES AND EXPRESS ION SYSTEMS FOR IT

ENDO-BETA-1,4-GLUKANASEN

REGULATORI SEQUENCES OF THE CELLULASEGENS CBH1 OUT TRICHODERMA VIRIDAE AND WHEREUPON BASED SYSTEM FOR THE MASS PRODUCTION OF PROTEINS AND PEPTIDEN

COMPACT DETERGENT COMPOSITIONS WITH HIGH-ACTIVITY CELLULASEN

ENDO-1,4-BETA-GLUKANASE

ENDO-BETA-1,4-GLUKANASE AND YOUR APPROPRIATE DNA

ENZYMHALTIGER FODDER ADDITIVE AND THIS CONTAINING FODDER

USE OF AN ENZYMATIC COMPOSITION AND PROCEDURE FOR AFFINAGE OF FRESH WOOD

SALT-STABILIZED ENZYME PREPARATION

METHOD FOR THE TREATMENT OF CELLULOSEHALTIGEN TEXTILES USING CELLULASE A FUSION PROTEIN, WHICH CONTAINS TWO CELLULOLYTI CATALYTIC CENTERS

ALKALINE CELLULASE AND PROCEDURES FOR THEIR PRODUCTION

USE OF THE GLUCOAMYLASEPROMOTORS OUT APERGILLUS

TRICHODERMA CELLULASE WITH HIGH MOLECULAR WEIGHT

ALKALINE CELLULASE AND PROCEDURES FOR THEIR PRODUCTION

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

AN ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY

Production of cellulase in plastids of transgenic plants

GENETICALLY-ENGINEERED STRAIN OF SYNECHOCYSTIS PCC6803 FOR PRODUCING CELLULASE AND CONSTRUCTION METHOD THEREFOR

Abstract The present invention provides a genetically-engineered strain of synechocystis PCC6803 for producing cellulase and a construction method therefor and relates to the field of industrial microorganisms.The present invention integrates the genes of the cellulose exonuclease CBH II into the genome of synechocystis PCC6803 through the homologous recombination technology, the synechocystis PCC6803 strain PSNC II obtained through the method can produce cellulase, and under identical culture conditions, the activity of the cellulase produced by the strain is obviously superior to that of a wild strain. The strain PSNC II capable of producing cellulase obtained in the present invention is of high theoretical and practical significance to the construction of genetically-engineered bacteria for producing cellulase.

Immobilized enzyme complexes and related methods

Immobilized enzyme complexes (IEC) with enzymes that are non-covalently linked to matrices are provided along with methods for making the same. Methods of using the IEC for a wide variety of industrial enzymatic processes are also provided. Methods of converting cellulosic biomass and methods of effecting blood type conversions with the IEC are amongst the methods disclosed.

Cellulolytic enzyme compositions and uses thereof

CELLULOLYTIC ENZYME COMPOSITIONS AND USES THEREOF The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

NOVEL GLYCOSYL HYDROLASE ENZYMES AND USES THEREOF

The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars. WO 2011/038019 PCT/US2010/049849 Cellulase Activity Assay 0.7% PASC, 500C, 2 Hours c 0.20 y = 0.0272x + 0.0146 0.10R2 =0.9955 GC220 (mg Protein/G PASC) FG. 2%5 AfuXyn5 ...

Cellulase composition for treatment of cellulose-containing textile materials

Mutant egiii cellulase, dna encoding such egiii compositions and methods for obtaining same

Treatment of cellulosic material and enzymes useful therein

The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.

Method of producing fungal culture

It is intended to provide a method of producing a fungal culture obtained by culturing a fungus in a liquid medium, which contains as a culture material at least one member selected from among cereals, beans, potatoes, amaranthus and quinua, wherein the speed of releasing a nutrient in the culture material into the culture system is controlled to thereby control the productivity of an enzyme (in particular, a starch digesting enzyme, a vegetable fiber digesting enzyme or a protease) of the fungal culture. Namely, a method of producing a fungal culture characterized by comprising culturing a fungus by using a liquid medium, which contains as a culture material at least one member selected from among cereals, beans, potatoes, amaranthus and quinua, while controlling the speed of releasing a nutrient in the culture material into the culture system to thereby control the enzyme productivity of the fungal culture.

Method for production of beta-glucanase and xylanase, and liquid culture medium

The object is to produce a cellulase having an excellent ability of decomposing a cellulosic resource containing xylan at low cost. Disclosed is a method for producing -glucanase and xylanase, which comprises the step of culturing a microorganism belonging to the genus by using a liquid culture medium containing (a) a pulp derived from paper which has not been heated or treated with an alkali as a carbon source and (b) an ammonia nitrogen or an amino nitrogen as a nitrogen source.

Cellulases from rumen

Cellulase variants with improved expression, activity and/or stability, and use thereof

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.

Methods of increasing the cellulolytic enhancing activity of a polypeptide

The present invention relates to methods of increasing the activity of a polypeptide having cellulolytic enhancing activity, comprising: adding a soluble activating divalent metal cation to a composition comprising the polypeptide having cellulolytic enhancing activity, wherein the presence of the soluble activating divalent metal cation and the polypeptide having cellulolytic enhancing activity increases degradation or conversion of a cellulose-containing material by a cellulolytic enzyme composition compared to the polypeptide having cellulolytic enhancing activity without the soluble activating divalent metal cation. The present invention also relates to compositions, methods for degrading or converting a cellulose-containing material, and methods for producing a fermentation product.

Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars

The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having β-glucosidase activity, methods for hydrolyzing biomass material, and methods for improving the stability and saccharification efficacy of a composition comprising such β-glucosidase polypeptides and/or activity.

Cellulolytic enzyme compositions and uses thereof

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

Enzyme systems for saccharification of plant cell wall polysaccharides

Process for production of an enzyme product

The invention relates to a process for production of an enzyme product having a plurality of enzyme activities obtained by fermentation of an Aspergillus strain.

Processing cellulosic biomass

Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol are disclosed herein, along with plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols than are typically employed are also disclosed.

Novel Variant Hypocrea Jecorina CBH2 Cellulases

Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.

Enhanced cellulase expression in s. degradans

The invention provides organisms and methods of using and making organisms with enhanced cellulase expression.

Endoglucanase variants

The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides having Beta-Glucosidase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Variant humicola grisea cbh1.1

Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.

Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.

Polypeptides having endoglucanase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Cellobiohydrolase Variants and Polynucleotides Encoding Same

The present invention relates to variants of a parent cellobiohydrolase. The present invention also relates to polynucleotides encoding the cellobiohydrolase variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the cellobiohydrolase variants.

Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity

The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.

Method for improved protein production in filamentous fungi

The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.

Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof

The invention relates to a polypeptide which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having cellulolytic enhancing activity , selected from the group consisting of:(a) a polypeptide comprising an amino acid sequence having at least 60% identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1 or SEQ ID NO: 3, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO: 1 or SEQ ID NO: 3, or (iii) a full-length complementary strand of (i) or (ii);(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% identity to the mature polypeptide coding sequence of SEQ ID NO: 1; and(d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.2. The polypeptide of claim 1 , comprising or consisting of the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4; or a fragment thereof having cellulolytic enhancing activity.3E. coliE. coli. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid plasmid pSMai190 which is contained in NRRL B-50083 or plasmid pSMai192 which is contained in NRRL B-50085.4. An isolated polynucleotide comprising a nucleotide sequence that encodes the polypeptide .5. A nucleic acid construct comprising the polynucleotide of operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.6. A recombinant host cell comprising the nucleic acid construct of .7. A method of ...

Variants of Glycoside Hydrolases

The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences. 1. An isolated variant of a parent glycoside hydrolase , comprising a substitution at a position corresponding to position 373 of SEQ ID NO: 2 , wherein the variant comprises an amino acid sequence that has at least 90% sequence identity to SEQ ID NO: 2 , and wherein the variant has glycoside hydrolase activity.2. The variant of claim 1 , which comprises an amino acid sequence that has at least 95% sequence identity to SEQ ID NO: 2.3. The variant of claim 1 , which comprises an amino acid sequence that has at least 97% sequence identity to SEQ ID NO: 2.4. The variant of claim 1 , wherein the parent glycoside hydrolase comprises SEQ ID NO: 2 claim 1 , or a fragment thereof that has glycoside hydrolase activity.5. The variant of claim 1 , wherein the parent glycoside hydrolase comprises SEQ ID NO: 2.6. The variant of claim 1 , wherein the substitution is His.7. The variant of claim 1 , wherein the substitution is N373H.8. The variant of claim 1 , further comprising a substitution at a position corresponding to position 21 claim 1 , 94 ...

Novel expression-regulating sequences and expression products in the field of filamentous fungi

The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.

NOVEL GLYCOSYL HYDROLASE ENZYMES AND USES THEREOF

The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars. 1. A non-naturally occurring composition comprising:{'i': 'Trichoderma reesei', 'a. a first polypeptide or a variant thereof having β-xylosidase activity, L-α-arabinofuranosidase activity, or both β-xylosidase activity and L-α-arabinofuranosidase activity, selected from a Fv43D, Fv3A, Pf43A, Fv43E, Fv39A, Fv43A, Fv43B, Pa51A, Fo43A, Gz43A, Bxl1, Fv51A, Af43A, or Pf51A polypeptide; and'}{'i': Trichoderma', 'Aspergillus, 'b. a second polypeptide or a variant thereof having xylanase activity, selected from a xylanase or an xylanase;'}wherein the composition is capable of converting 60% or more of the hemicellulose xylan from a biomass into xylose.24-. (canceled)5T. reesei. The composition of claim 1 , wherein the first polypeptide is selected from a Fv43D claim 1 , Fv3A claim 1 , Pf43A claim 1 , Fv43E claim 1 , Fv39A claim 1 , Fo43A claim 1 , Gz43A claim 1 , or Bxl1 claim 1 , or Fv43A claim 1 , further comprising a third polypeptide or a variant thereof having L-α-arabinofuranosidase activity claim 1 , selected from a Fv51A claim 1 , Af43A claim 1 , Fv43B claim 1 , Pa51A or Pf51A polypeptide.6. (canceled)7Trichoderma reesei. The composition of claim 1 , further comprising a third polypeptide having β-xylosidase activity claim 1 , wherein the first polypeptide is an Fv3A polypeptide or an Fv43A polypeptide claim 1 , and the third polypeptide is an Fv43D claim 1 , Pa51A claim 1 , Gz43A claim 1 , Bxl1 claim 1 , Pf43A claim 1 , Fv43E claim 1 , Fv39A claim 1 , Fo43A claim 1 , or Fv43B polypeptide.8. (canceled)9. The composition of claim 1 , wherein claim 1 , if present:(a) the Fv43D polypeptide has at least 90% sequence identity to SEQ ID NO:28, or to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of ...

"Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And An Organic Compound And Uses Thereof"

The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and an organic compound. The present invention also relates to methods of using the compositions. 1. An aqueous composition comprising: (a) a polypeptide having cellulolytic enhancing activity and (b) an organic compound , wherein the combination of the polypeptide having cellulolytic enhancing activity and the organic compound enhances hydrolysis of a cellulosic material by a cellulolytic enzyme.2. The composition of claim 1 , wherein the organic compound comprises a pKa that is less than or about equal to the pH of the composition.3. The composition of or claim 1 , wherein the pH of the composition is greater than or equal to about 3.0 claim 1 , 3.5 claim 1 , 4.0 claim 1 , 4.5 claim 1 , 5.0 claim 1 , 5.5 claim 1 , 6.0 claim 1 , 6.5 claim 1 , 7.0 claim 1 , 7.5 claim 1 , or 8.0.4. The composition of claim 1 , wherein the organic compound comprises a pKa less than or equal to about 8.0 claim 1 , 7.5 claim 1 , 7.0 claim 1 , 6.5 claim 1 , 6.0 claim 1 , 5.5 claim 1 , 5.0 claim 1 , 4.5 claim 1 , 3.5 claim 1 , 3.0 claim 1 , or 2.5.5. The composition of claim 1 , wherein greater than or about 95% claim 1 , 90% claim 1 , 85% claim 1 , 80% claim 1 , 75% claim 1 , 70% claim 1 , 65% claim 1 , 60% claim 1 , 55% claim 1 , 50% claim 1 , 45% claim 1 , 40% claim 1 , 35% claim 1 , 30% claim 1 , 25% claim 1 , 20% claim 1 , 15% claim 1 , 10% claim 1 , 5% claim 1 , 2.5% claim 1 , or 1% of the organic compound is in proton dissociated form.6. The composition of claim 5 , wherein the proton dissociated form of the organic compound is neutral or anionic with respect to the proton dissociated moiety.7. The composition of claim 1 , wherein the organic compound comprises a carboxylic acid moiety claim 1 , a lactone moiety claim 1 , a phenolic moiety claim 1 , a flavonoid moiety claim 1 , or a combination thereof.8. The composition of claim 1 , wherein the organic compound is ...

Polypeptides Having Cellobiohydrolase Activitiy and Polynucleotides Encoding Same

The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having cellobiohydrolase activity , selected from the group consisting of:(a) a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1;(c) a variant comprising a substitution, deletion, and/or insertion of one or more amino acids of the mature polypeptide of SEQ ID NO: 2; and(d) a fragment of a polypeptide of (a), (b), or (c) that has cellobiohydrolase activity.2. The polypeptide of claim 2 , comprising or consisting of SEQ ID NO: 2 or the mature polypeptide thereof.3E. coli. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pGEM-T-CBHII46949-2 which is contained in DSM 24143.4. An isolated polypeptide comprising a catalytic domain selected from the group consisting of:(a) a catalytic domain having at least 96% sequence identity to amino acids 112 to 469 of SEQ ID NO: 2;(b) a catalytic domain encoded by a polynucleotide having at least 96% sequence identity to nucleotides 430 to 1749 of SEQ ID NO: 1 or the cDNA sequence thereof;(c) a variant of amino acids 112 to 469 of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion of one or more positions; and(d) a fragment of a catalytic domain of (a), (b), or (c), which has cellobiohydrolase activity.5. The polypeptide of claim 4 , comprising or consisting of amino acids 112 to 469 of SEQ ID NO: 2.6E. coli. The polypeptide of claim 4 , which is encoded by the polynucleotide contained in plasmid pGEM-T-CBHII46949-2 which is contained in DSM 24143.7. The polypeptide ...

Method and Compositions for Improved Lignocellulosic Material Hydrolysis

A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of sp. ActE secretome such that at least partial lignocellulosic digestion occurs. 1Streptomyces. A method of digesting a lignocellulosic material , comprising the step of exposing the material to an effective amount of sp: ActE secretome preparation such that at least partial lignocellulosic digestion occurs.2Streptomyces. The method of wherein the preparation is a supernatant preparation obtained from a sp. ActE culture.3StreptomycesStreptomyces. The method of claim 1 , wherein the preparation is obtained from sp. ActE grown on a substrate wherein at least 40% claim 1 , preferably 85% claim 1 , of sp. ActE's carbon source in the substrate is derived from a material selected from the group consisting of cellulose claim 1 , cellulose/hemicelluloses mixture claim 1 , hemicelluloses claim 1 , xylan claim 1 , non-wood biomass claim 1 , wood biomass claim 1 , and chitin.4. The method of claim 1 , wherein the lignocellulosic material is selected from the group consisting of materials that comprise at least 75% cellulose claim 1 , cellulose/hemicelluloses claim 1 , xylose claim 1 , biomass and chitin.5Streptomyces. A purified preparation comprising the sp. ActE secretome.6Streptomyces. The preparation of wherein the preparation is a supernatant preparation obtained from a sp. ActE culture.7StreptomycesStreptomyces. The preparation of wherein sp. ActE is grown on a substrate wherein at least 40% claim 5 , preferably 85% claim 5 , of sp. ActE's carbon source in the substrate is derived from a material selected from the group consisting of cellulose claim 5 , cellulose/hemicelluloses mixture claim 5 , hemicelluloses claim 5 , xylan claim 5 , non-wood biomass claim 5 , wood biomass claim 5 , and chitin.8. A composition useful for digesting lignocellulosic material comprising SActE0237 (GH6) (SEQ ID NOs:1 and 17) gene ...

Polypeptides Having Endoglucanase Activity And Polynucleotides Encoding Same

The present invention provides isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cell comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having endoglucanase activity , selected from the group consisting of:(a) a polypeptide having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1;(c) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions, wherein the variant has endoglucanase activity; and(d) a fragment of a polypeptide of (a), (b), or (c) that has endoglucanase activity.2. The polypeptide of claim 1 , comprising or consisting of SEQ ID NO: 2 or the mature polypeptide thereof.3E. coli. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pGEM-T-Ppin13 which is contained in DSM 24144.4. An isolated polypeptide comprising a catalytic domain selected from the group consisting of:(a) a catalytic domain having at least 91% sequence identity to amino acids 24 to 419 of SEQ ID NO: 2;(b) a catalytic domain encoded by a polynucleotide having at least 91% sequence identity to nucleotides 70 to 1257 of SEQ ID NO: 1;(c) a variant of amino acids 24 to 419 of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions, wherein the variant has endoglucanase activity; and(d) a fragment of a catalytic domain of (a), (b), or (c), which has endoglucanase activity.5. An isolated polypeptide comprising a cellulose binding domain selected from the group consisting of:(a) a cellulose binding domain having at least 91% sequence identity to amino acids 463 to 498 of SEQ ID NO: 2;(b) a cellulose ...

HYDROLYTIC ENZYME MIXTURES FOR SACCHARIFICATION OF LIGNOCELLULOSIC POLYSACCHARIDES

The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose. These systems have a number of applications. Some embodiments relate to a method of producing ethanol using the cell wall degradative systems of the present invention. 1. A method for producing ethanol from lignocellulosic material , comprising treating lignocellulosic material with an effective saccharifying amount of one or more Compounds listed in to obtain saccharides and converting the saccharides to produce ethanol.2Microbulbifer degradans. The method of claim 1 , wherein the at least one of the compounds listed in are from 2-40.3. The method of claim 1 , wherein the lignocellulosic material is treated with an effective saccharifying amount of all of the compounds listed in .4. The method of claim 1 , wherein the one or more compounds listed in are present in dry form claim 1 , in a buffer claim 1 , or in the form of a paste claim 1 , paint claim 1 , or micelle.5. The method of claim 1 , wherein the one or more compounds listed in are in a system consisting essentially of one or more compounds listed in .6. The method of claim 1 , wherein the one or more compounds listed in are present in a system further comprising metal ions claim 1 , chelators claim 1 , detergents claim 1 , organic ions claim 1 , inorganic ions claim 1 , or one or more additional proteins.7. The method of claim 6 , where the one or more additional proteins are biotin and/or albumin.8. The method of claim 1 , wherein the treating is conducted in a marine environment.9. The method of claim 8 , wherein the treating is conducted under water.10. The method of claim 1 , wherein the one or more compounds listed in is cellulase cel5A listed in .11. A method for producing ethanol from lignocellulosic material claim 1 , comprising contacting lignocellulosic material with a microorganism expressing an effective saccharifying amount of one or more ...

Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same

The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides Having Endoglucanase Activity And Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides Having Hemicellulolytic Activity And Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having hemicellulolytic activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having hemicellulolytic activity , selected from the group consisting of:(a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 20; at least 70% sequence identity to the mature polypeptide of SEQ ID NO: 22 or SEQ ID NO: 24; at least 75% sequence identity to the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, or SEQ ID NO: 18; at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 16; or at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 4, SEQ ID NO: 12, or SEQ ID NO: 14;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, or SEQ ID NO: 23, (ii) the cDNA thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 19 or the cDNA sequence thereof; at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 21, or SEQ ID NO: 23, or the cDNA sequence thereof; at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, or SEQ ID NO: 17, or the cDNA sequence thereof; at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 15 or the cDNA sequence thereof; or at least 85% sequence identity to the mature polypeptide coding ...

Heterologous Expression of Fungal Cellobiohydrolase 2 Genes in Yeast

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms , and sp. in host cells, such as the yeast . The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems. 1Cochliobolus heterostrophus; Gibberella zeae; Irpex lacteus; VolvariellaPiromyces. An isolated polynucleotide comprising a nucleic acid which encodes a cellobiohydrolase or a fragment thereof , wherein said nucleic acid is codon-optimized for expression in a yeast strain and wherein the cellobiohydrolase is selected from the group consisting of a: ; and sp. cellobiohydrolase.2. (canceled)3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. (canceled)9. (canceled)10. (canceled)11. The polynucleotide of claim 1 , wherein the fragment of the cellobiohydrolase is a cellobiohydrolase signal peptide.12. (canceled)13. The polynucleotide of claim 1 , wherein the fragment of the cellobiohydrolase is a cellobiohydrolase cellulose-binding module (CBM).14. (canceled)15. The polynucleotide of claim 1 , wherein the fragment of the cellobiohydrolase is a GH family 6 domain.16. (canceled)17. (canceled)18. (canceled)19. (canceled)20. (canceled)21. (canceled)22. (canceled)23. (canceled)24. (canceled)25. (canceled)26. The polynucleotide of claim 1 , wherein said polynucleotide is operably associated with a heterologous nucleic acid.27. The polynucleotide of claim 26 , wherein the heterologous nucleic acid encodes a signal peptide claim 26 , a secretion signal claim 26 , or a carbohydrate binding module.28. (canceled)29. (canceled)30. (canceled)31. (canceled)32. (canceled)33. (canceled)34. A vector comprising the polynucleotide of .35S. cerevisiaeS. cerevisiaeS. cerevisiaeS. cerevisiae. The ...

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

CELLULOYTIC ENZYMES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM

The invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, β-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention. 2. The isolated claim 1 , synthetic or recombinant nucleic acid of claim 1 , wherein the nucleic acid sequence comprises the sequence of SEQ ID NO:519.3. The isolated claim 1 , synthetic or recombinant nucleic acid of claim 1 , wherein the sequence comparison algorithm is a BLAST version 2.2.2 algorithm where a filtering setting is set to blastall -p blastp -d “nr pataa”-F F claim 1 , and all other options are set to default. stylecl4. The isolated claim 1 , synthetic or recombinant nucleic acid of claim 1 , wherein the cellulase activity comprises an endocellulase activity claim 1 , an endoglucanase activity claim 1 , a cellobiohydrolase activity claim 1 , an β-glucosidase claim 1 , β-xylosidase claim 1 , a mannanase activity claim 1 , or any combination thereof.5. The isolated claim 1 , synthetic or recombinant nucleic acid of claim 1 , wherein the oligomerase activity comprises hydrolyzing (degrading) soluble oligomers to fermentable claim 1 , monomeric sugars.6. The isolated claim 1 , synthetic or recombinant nucleic acid of claim 1 , wherein the oligomerase activity comprises hydrolyzing (degrading) soluble cellooligsaccharides and arabinoxylan oligomers into monomers claim 1 , and optionally the monomers comprise xylose claim 1 , arabinose and glucose.7. The isolated claim 1 , ...

"Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same"

The present invention relates to polypeptide having cellulolytic enhancing activity variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. 1. A variant , comprising a substitution at one or more positions corresponding to positions 75 , 77 , 179 , 181 , and 183 of the mature polypeptide of SEQ ID NO: 2 , wherein the variant has cellulolytic enhancing activity.2. The variant of claim 1 , which is a variant of a parent polypeptide having cellulolytic enhancing activity selected from the group consisting of:(a) a polypeptide having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least low stringency conditions, e.g., medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof; and(d) a ...

Heterologous Expression of Fungal Cellobiohydrolase 2 Genes in Yeast

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms , and sp. in host cells, such as the yeast . The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems. 1Cochliobolus heterostrophus; Gibberella zeae; Irpex lacteus; VolvariellaPiromyces. An isolated polynucleotide comprising a nucleic acid which encodes a cellobiohydrolase or a fragment thereof , wherein said nucleic acid is codon-optimized for expression in a yeast strain and wherein the cellobiohydrolase is selected from the group consisting of a: ; and sp. cellobiohydrolase.2. (canceled)3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. (canceled)9. (canceled)10. (canceled)11. The polynucleotide of claim 1 , wherein the fragment of the cellobiohydrolase is a cellobiohydrolase signal peptide.12. (canceled)13. The polynucleotide of claim 1 , wherein the fragment of the cellobiohydrolase is a cellobiohydrolase CBM.14. (canceled)15. The polynucleotide of claim 1 , wherein the fragment of the cellobiohydrolase is a GH family 6 domain.16. (canceled)17. (canceled)18. (canceled)19. (canceled)20. (canceled)21. (canceled)22. (canceled)23. (canceled)24. (canceled)25. (canceled)26. The polynucleotide of claim 1 , wherein said polynucleotide is operably associated with a heterologous nucleic acid.27. The polynucleotide of claim 26 , wherein the heterologous nucleic acid encodes a signal peptide claim 26 , a secretion signal claim 26 , or a carbohydrate binding module.28. (canceled)29. (canceled)30. (canceled)31. (canceled)32. (canceled)33. (canceled)34. A vector comprising the polynucleotide of .35S. cerevisiaeS. cerevisiaeS. cerevisiaeS. cerevisiae. The vector of further comprising ...

Production of proteins in filamentous fungi

The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to be deficient or to overexpress specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host. 1. A method to genetically modify a filamentous fungus host for improved protein production , said method comprising:{'i': Trichoderma, Aspergillus, Fusarium, Neurospora, Talaromyces, Phanerochaete, Chrysosporium', 'Penicillium, 'genetically modifying a filamentous fungus host to be deficient, with reduced or lacking amount or activity, or to overexpress, to increased amount or activity, of one or more genes selected from the group consisting of genes tre108381 (SEQ ID NO:1), tre81972 SEQ ID NO:2), tre62244 (SEQ ID NO:3), tre76677 (SEQ ID NO:4), and tre47317 (SEQ ID NO:5); or the closest homologue of at least one of said genes in or ; or a fragment or derivative of any of said genes or other nucleotide sequence hybridizing under stringent conditions to at least one of said genes or said homologues, said host being capable of increased or decreased production of cellulase, hemicellulase, other proteins involved in the degradation of lignocellulosic material and/or other proteins as compared to the parental strain.'}2Trichoderma, Aspergillus, Fusarium, Neurospora, Talaromyces, Phanerochaete, ChrysosporiumPenicillium. The method according to claim 1 , wherein the filamentous fungus host is genetically modified to be deficient of one or more genes selected from the group consisting of genes tre108381 (SEQ ID NO:1) claim 1 , tre81972 SEQ ID NO:2) claim 1 , tre62244 (SEQ ID NO:3) claim 1 , tre76677 ...

VARIANT HUMICOLA GRISEA CBH1.1

Disclosed are variants of Cel7A (CBH1.1), CBH1 variant or CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted. 119-. (canceled)20H. jecorina. An isolated polynucleotide encoding a polypeptide having cellobiohydrolase I activity , wherein said polynucleotide is a CBH1 variant comprising a substitution at a position corresponding to one or more of T55E , T55K , S58T , Q101Y , Q101H , N250D , N250E , P265A , P265S and L288I of SEQ ID NO:10.21. A nucleic acid construct comprising the isolated polynucleotide of claim 20 , operably linked to one or more control sequences.22. A recombinant expression vector comprising the nucleic acid construct of .23. A recombinant host cell comprising the nucleic acid construct of or the recombinant expression vector of .24. A method of producing a polypeptide having cellobiohydrolase I activity claim 21 , said method comprising:{'claim-ref': {'@idref': 'CLM-00020', 'claim 20'}, 'a) transforming a host cell with a nucleic acid comprising the isolated polynucleotide of ;'}b) culturing the host cell under conditions suitable for producing the polypeptide; andc) recovering the polypeptide.25. A composition comprising a polypeptide having cellobiohydrolase I activity prepared by performing the method of .26. The composition of claim 24 , which is a fermentation broth produced by the recombinant host cell of .27. A method of applying the composition of or to convert biomass to sugars comprising contacting the biomass with the composition. This application claims priority to U.S. Provisional Application No. 60/459,734 filed Apr. 1, 2003 (Attorney Docket No. GC794P) herein incorporated by reference.Portions of this work were funded by Subcontract No. ZCO-0-30017-01 with the National Renewable Energy Laboratory under Prime Contract No. DE-AC36-99GO10337 with the U.S. Department of ...

INTEIN-MODIFIED ENZYMES, THEIR PRODUCTION AND INDUSTRIAL APPLICATION

A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided. 1. An intein modified protein comprising a target protein and an intein inserted into the target protein at a site i) immediately prior to a cysteine , threonine , or serine residue of the target protein , ii) within ten angstroms from an active site of the target protein , and iii) within 10 amino acids of a loop-β-sheet junction or within 10 amino acids of a loop-α-helix junction of the target protein , wherein the target protein is a xylanase or a cellulase.2. The intein modified protein of claim 1 , wherein the site includes an insertion site cassette having amino acid positions −3 claim 1 , −2 claim 1 , −1 claim 1 , 0 claim 1 , 1 claim 1 , and 2 where the 0 position is the cysteine claim 1 , theronine claim 1 , or serine and the insertion site cassette has a −3 claim 1 , −2 claim 1 , −1 claim 1 , 0 claim 1 , 1 claim 1 , 2 sequence selected from the group consisting of GGKCGG (SEQ ID NO: 1513) claim 1 , GGKSGG (SEQ ID NO: 1514) claim 1 , GGKTGG (SEQ ID NO: 1515) claim 1 , PGATSP (SEQ ID NO: 1516) claim 1 , PGATVP (SEQ ID NO: 1517) claim 1 , GAKSLG (SEQ ID NO: 1518) claim 1 , PGATSL (SEQ ID NO: 1519) claim 1 , PGASPL (SEQ ID NO: 1520) claim 1 , PGATGP (SEQ ID NO: 1521) claim 1 , AQRSLG (SEQ ID NO:1522) claim 1 , NQPSIV (SEQ ID NO: 1523) claim 1 , NQASIV (SEQ ID NO: 1524) claim 1 , PNMSSA (SEQ ID NO: 1525) claim 1 , GNHSSG (SEQ ID NO: 1526) ...

NOVEL CELLULASE DERIVED FROM THERMOSPOROTHRIX HAZAKENSIS

This invention provides a novel cellulase derived from . The cellulase derived from has enzyme activity on at least β-glucan, soluble cellulose, crystalline cellulose, phosphoric acid-swollen cellulose, and xylan. 1Thermosporothrix hazakensis. A cellulase derived from having enzyme activity on at least β-glucan , soluble cellulose , crystalline cellulose , phosphoric acid-swollen cellulose , and xylan.2Thermosporothrix hazakensisThermosporothrix hazakensis. The cellulase according to claim 1 , wherein the is the SK20-1strain (JCM 16142T=ATCC BAA-1881T).3. The cellulase according to or claim 1 , which retains enzyme activity at a temperature of at least 10° C. to 80° C.4. The cellulase according to or claim 1 , which retains enzyme activity at a pH of at least 2 to 11.5. The cellulase according to or claim 1 , which retains enzyme activity in the presence of an organic solvent at 0% to 25% (v/v) or higher concentration.6. The cellulase according to claim 5 , wherein the organic solvent is selected from the group consisting of toluene claim 5 , acetone claim 5 , chloroform claim 5 , butanol claim 5 , hexane claim 5 , and DMSO.7. The cellulase according to or claim 5 , which retains enzyme activity in the presence of ethanol at 0% to 50% (v/v) or higher concentration.8. The cellulase according to or claim 5 , which retains enzyme activity in the presence of salt at 0% to 25% (v/v) or higher concentration.9. The cellulase according to or claim 5 , which comprises one or more hydrolases selected from the group consisting of hydrolases comprising polypeptides represented by the amino acid sequences below:(I) a polypeptide comprising the amino acid sequence as shown in SEQ ID NO: 1, a polypeptide comprising an amino acid sequence having deletion, substitution, insertion, or addition of one or several amino acids in the amino acid sequence as shown in SEQ ID NO: 1 and having cellulase activity, or a polypeptide comprising an amino acid sequence having at least 90% identity ...

Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having cellobiohydrolase activity selected from the group consisting of:(a) a polypeptide having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2 or a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 4;(b) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof or a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3 or the genomic DNA sequence thereof;(c) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO: 2 or of SEQ ID NO: 4; and(d) a fragment of the polypeptide of (a), (b), or (c) that has cellobiohydrolase activity.2. The polypeptide of claim 1 , having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2.3. The polypeptide of claim 1 , having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 4.4. The polypeptide of claim 1 , comprising or consisting of SEQ ID NO: 2 or SEQ ID NO. 4.5. The polypeptide of claim 4 , comprising or consisting of the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.6. The polypeptide of claim 5 , wherein the mature polypeptide is amino acids 19 to 455 of SEQ ID NO: 2 or 26 to 537 of SEQ ID NO: 4.7. An isolated polypeptide comprising a catalytic domain selected from the group consisting of:(a) a catalytic domain having at least 90% sequence identity to the catalytic domain of SEQ ID NO: 2 or a catalytic domain ...

METHOD OF IMPROVING THE ACTIVITY OF CELLULASE ENZYME MIXTURES IN THE SACCHARIFICATION (LIGNO)CELLULOSIC MATERIAL

The present invention relates to modified filamentous fungal organisms having improved activity profiles with respect to the conversion of complex carbohydrates into simple sugars from cellulosic materials, including fungal organisms belonging to a genus selected from the group consisting of: , and , plus anamorphs and teleomorphs thereof. Filamentous fungal organisms having improved activity profiles are obtained by modifying genes encoding enzymes involved in the production of cellobionolactone, cellobionic acid, gluconolactone, gluconic acid, and related products, by a variety of mutagenic methods, resulting in nucleotide substitutions, insertions, and deletions, increasing the level of saccharification in enzyme mixtures obtained from the modified organisms. 1. A modified fungus comprising one or more genes encoding enzymes having one or more cellulase or hemicellulase activities;wherein said fungus comprises one or more modified genes encoding enzymes responsible for the production of one or more products selected from cellobionolactone, cellobionic acid, gluconolactone, and gluconic acid;wherein the level of expression of said modified genes is reduced or eliminated or the level of activity of modified enzymes encoded by said modified genes is reduced or eliminated compared to the endogenous level of expression or activity in a parent fungus lacking one or more of said modifications.2Chrysosporium, Thielavia, Talaromyces, Thermomyces, Thermoascus, Neurospora, Aureobasidium, Filibasidium, Piromyces, Corynascus, Cryplococcus, Acremonium, Tolypocladium, Scytalidium, Schizophyllum, Sporotrichum, Penicillium, Gibberella, Myceliophthora, Mucor, Aspergillus, Fusarium, HumicolaTrichodermaTalaromyces emersonii. The modified fungus of claim 1 , wherein said fungus is a filamentous fungus from a genus or genus and species selected from the group consisting of claim 1 , and claim 1 , and claim 1 , plus anamorphs and teleomorphs claim 1 , and derivatives thereof. ...

Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 125-. (canceled)26. An isolated polypeptide having cellobiohydrolase activity , selected from the group consisting of:(a) a polypeptide having at least 81% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; or the cDNA sequence thereof;(d) a fragment of the polypeptide of (a), (b), or (c) that has cellobiohydrolase activity.27. The polypeptide of claim 26 , comprising or consisting of SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2.28. The polypeptide of claim 27 , wherein the mature polypeptide is amino acids 19 to 464 of SEQ ID NO: 2.29. The polypeptide of claim 26 , which is a fragment of SEQ ID NO: 2 claim 26 , wherein the fragment has cellobiohydrolase activity.30. An isolated polypeptide comprising a catalytic domain selected from the group consisting of:(a) a catalytic domain having at least 81% sequence identity to the catalytic domain of SEQ ID NO: 2;(b) a catalytic domain encoded by a polynucleotide having at least 60% sequence identity to the catalytic domain coding sequence of SEQ ID NO: 1;(c) a fragment of a catalytic domain of (a) or (b), which has cellobiohydrolase activity.31. The polypeptide of claim 30 , comprising or consisting of the catalytic domain of SEQ ID NO: 2.32. The polypeptide of claim 31 , wherein the catalytic domain is amino acids 105 to ...

Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains. 1. An isolated polypeptide having endoglucanase activity , selected from the group consisting of:(a) a polypeptide having at least 75% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under medium-high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, or (ii) the full-length complement of (i);(c) a polypeptide encoded by a polynucleotide having at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1;(d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or several positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has endoglucanase activity.2. (canceled)3. The polypeptide of claim 1 , comprising or consisting of SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2.4. The polypeptide of claim 3 , wherein the mature polypeptide is amino acids 19 to 477 of SEQ ID NO: 2.5. An isolated polypeptide comprising a catalytic domain selected from the group consisting of:(a) a catalytic domain having at least 80% sequence identity to amino acids 19 to 397 of SEQ ID NO: 2;(b) a catalytic domain encoded by a polynucleotide that hybridizes under high, or very high stringency conditions with (i) nucleotides 55 to 1191 of SEQ ID NO: 1, or (ii) the full-length complement of (i);(c) a catalytic domain encoded by a polynucleotide having at least 80% sequence identity to the catalytic domain of SEQ ID NO: 1;(d) a variant ...

TREATMENT OF SANFILIPPO SYNDROME TYPE B

Among other things, the present invention provides methods and compositions of treating Sanfilippo syndrome type B (Sanfilippo B) by, e.g., intrathecal (IT) administration of a Naglu protein. A suitable Naglu protein can be a recombinant, gene-activated or natural protein. In some embodiments, a suitable Naglu protein is a recombinant Naglu protein. In some embodiments, a recombinant Naglu protein is a fusion protein containing a Naglu domain and a lysosomal targeting moiety. In some embodiments, the lysosomal targeting domain is an IGF-II moiety.

Processes for Treating Textile with Polypeptide Having Cellulolytic Enzyme Enhancing Activity

The present invention relates to the use of glycosyl hydrolase family (61) polypeptides in the presence of cellulases for textile manufacture as well as a textile composition comprising glycosyl hydrolase family (61) polypeptides and cellulases. 1. A method for treating textile with a glycosyl hydrolase family 61 polypeptide in the presence of a cellulase in an aqueous solution.2. The method according to wherein the method is applied in a biostoning process.3. The method according to claim 1 , wherein the textile is dyed cellulosic or cellulose-containing fabric claim 1 , preferably denim fabric claim 1 , more preferably indigo dyed denim fabric.4. The method according to claim 1 , wherein the method is applied in a biopolishing process.5. The method according to claim 4 , wherein the textile is yarn claim 4 , fabric or garment.6. The method according to claim 1 , wherein the cellulase is an endoglucanase (EC 3.2.1.4).7. The method according to claim 1 , wherein the aqueous solution further comprises one or more enzymes selected from the group consisting of proteases claim 1 , lipases claim 1 , cutinases claim 1 , amylases claim 1 , pectinases claim 1 , hemicellulases claim 1 , oxidoreductases claim 1 , peroxidases claim 1 , laccases claim 1 , and transferases.8. The method according to claim 1 , wherein a cosubstance is used together with a glycosyl hydrolase family 61; preferably the cosubstance is cysteine.9. The method according to claim 1 , wherein the glycosyl hydrolase family 61 polypeptide is applied in the range of from 0.001 to about 10 milligram enzyme protein per gram of fabric.10. The method according to (Original) claim 1 , wherein the cellulase is applied in the range from 0.001 to about 10 milligram enzyme protein per gram of fabric.11. The method according to claim 1 , wherein the method is conducted in the pH range of from about pH 3 to about pH 11.12. The method according to claim 1 , wherein the method is conducted in the temperature range of 10- ...

MUTANT CELLOBIOHYDROLASE

The invention relates to Mutant cellobiohydrolase, being a mutant of SEQ ID NO:1, having a substitution at position N247(I,F,H,W) of SEQ ID NO: 1, wherein the mutant cellobiohydrolase has at least 50% sequence identity with SEQ ID NO: 1, and wherein the mutant cellobiohydrolase has CBHI activity. 1. A mutant cellobiohydrolase , being a mutant of SEQ ID NO: 1 , comprising a substitution at position N247(I ,F ,H ,W) of SEQ ID NO: 1 , wherein said mutant cellobiohydrolase comprises at least 50% sequence identity with SEQ ID NO: 1 , and wherein said mutant cellobiohydrolase comprises CBHI activity.2. The mutant cellobiohydrolase according to claim 1 , wherein said mutant comprises substitution N247F.3. The mutant cellobiohydrolase according to claim 1 , wherein said mutant comprises substitution N247H.4. The mutant cellobiohydrolase according to claim 1 , wherein CBH-I comprises the amino acid sequence as set out in SEQ ID NO: 1 and said mutant has a substitution or deletion at a position corresponding to at least one of residues F427 claim 1 , K163 claim 1 , G357 claim 1 , S36 claim 1 , D77 claim 1 , and/or Q232.5. The mutant cellobiohydrolase according to claim wherein said mutant comprises at least one of the following substitutions: F4271 claim 1 , K163N claim 1 , G357R claim 1 , S36E claim 1 , D77M and/or Q232A.6. The mutant cellobiohydrolase according to claim 1 , wherein CBH-I comprises the amino acid sequence as set out in SEQ ID NO: 1 and said mutant comprises a substitution or deletion at a position corresponding to at least one of residues T52 claim 1 , V101 claim 1 , S192 claim 1 , T198 claim 1 , T246 claim 1 , T344 claim 1 , D346 claim 1 , A375 and/or A376.7. The mutant cellobiohydrolase according to claim 6 , wherein said mutant comprises at least one of the following substitutions: T52(G claim 6 ,M claim 6 ,Y claim 6 ,D claim 6 ,H claim 6 ,K claim 6 ,R) claim 6 , V101(T claim 6 ,I claim 6 ,F claim 6 ,H) claim 6 , S192(A claim 6 ,I claim 6 ,F claim 6 ,Q ...

Endoglucanases

The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity, which perform very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications. 1125-. (canceled)126. An isolated polypeptide having cellulase activity , selected from the group consisting of:(a) a polypeptide encoded by a nucleic acid sequence with hybridizes with SEQ ID NO: 7, 9, 13, 15, 21, or 25 carried out in 2×SSC (Sambrook, 1989), 5×Denhardt's solution (Sambrook, 1989), 0.5% (w/v) SDS, 100 micrograms/ml denatured salmon sperm DNA for 20 h at 65° C. followed by washes in 5×SSC at 25° C. (2×15 min), 2×SSC, 0.5% SDS at 65° C. (30 min), 0.2×SSC, 0.5% SDS at 65° C. (30 min) and finally in 5×SSC (2×15 min) at 25° C.;(b) a polypeptide having an amino acid sequence which has a degree of identity of at least 90% with SEQ ID NO: 8, 10, 14, 16, 22, or 26, wherein the degree of identity is determined by means of GAP provided in the GCG program package using settings of a GAP creation penalty of 3.0 and GAP extension penalty of 0.1; and(c) a functional fragment of SEQ ID NO: 12.127. A polypeptide of claim 126 , having endoglucanase activity.128. A laundry composition comprising a polypeptide of and a component selected from the group consisting of a surfactant claim 126 , a builder compound claim 126 , and a fabric softening agent.129. The laundry composition of claim 128 , which further comprises one or more enzymes selected from the group consisting of proteases claim 128 , amylases claim 128 , lipases claim 128 , cellulases claim 128 ...

CELLULASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM

This invention relates to molecular and cellular biology and biochemistry. In one aspect, the invention provides polypeptides having cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or β-glucosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or β-glucosidase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. 1. An isolated or recombinant nucleic acid comprising(a) a nucleic acid sequence having at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more or complete sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53, SEQ ID NO:55, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:65, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, SEQ ID NO:79, SEQ ID NO:81, SEQ ID NO:83, SEQ ID NO:85, SEQ ID NO:87, SEQ ID NO:89, SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:99, SEQ ID NO:101, SEQ ID NO:103, SEQ ID NO:105, SEQ ID NO:107, SEQ ID NO:109, SEQ ID NO:111, SEQ ID NO:113, SEQ ...

Beta-glucosidase enhanced filamentous fungal whole cellulase compostions and methods of use

The present disclosure provides beta-glucosidase enhanced filamentous fungal whole cellulase compositions. Also provided are methods of hydrolyzing a cellulosic material with beta-glucosidase enhanced whole cellulase compositions. The present disclosure further provides methods of decreasing the amount of a whole cellulase required to hydrolyze a cellulosic material by adding an effective amount beta-glucosidase.

Cellulase Enzyme Mixtures For Depilling and Uses Thereof

The present invention relates to a depilling composition comprising an enzyme mixture that comprises a Family 45 cellulase enzyme component and one or more additional cellulase enzyme components selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof. The enzyme mixture may be characterized in that the Family 45 and the Family 5 cellulase enzyme components or the Family 45 and the Family 6 cellulase enzyme components are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity. The enzyme mixture may be secreted by a genetically modified microbe overexpressing theforegoing cellulase enzyme components. Also provided is a process for depilling that comprises a step of contacting cellulose-containing goods with the depilling composition. 1. A depilling composition comprising an enzyme mixture , which enzyme mixture comprises a Family 45 cellulase enzyme component and one or more additional cellulase enzyme components selected from a Family 5 cellulase , a Family 6 cellulase or a combination thereof , wherein said enzyme mixture is secreted by a genetically modified microbe overexpressing (i) a Family 45 cellulase gene encoding said Family 45 cellulase enzyme; and (ii) a gene or genes encoding the one or more additional cellulase enzyme component selected from a Family 5 cellulase , a Family 6 cellulase or a combination thereof , wherein the Family 45 cellulase has at least 75% sequence identity to amino acids 1-213 of SEQ ID NO:7 (HiCel45) or at least 20% sequence identity to amino acids 1-166 of SEQ ID NO:4 (TrCel45).2. The depilling composition of claim 1 , wherein said enzyme mixture is characterized in that the Family 45 and the Family 5 cellulase enzyme components or the Family 45 and the Family 6 cellulase enzyme components are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity.3. The depilling composition of claim 1 , comprising both the ...

Signal Peptide for Producing a Polypeptide

The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.

CHIMERIC ENZYMES WITH IMPROVED CELLULASE ACTIVITIES

Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose. 1Clostridium thermocellumCaldicellulosiruptor bescii. An isolated nucleic acid molecule encoding a chimeric CbhA polypeptide comprising domains from CbhA and Ce1A polypeptides.2Caldicellulosiruptor bescii. The isolated nucleic acid molecule of claim 1 , wherein the chimeric CbhA polypeptide comprises the linker domain from the Ce1A polypeptide.3. The isolated nucleic acid molecule of claim 1 , wherein the chimeric CbhA polypeptide has a cellulase activity at least 2-fold greater than the wild-type CbhA polypeptide.4. The isolated nucleic acid molecule of claim 1 , wherein the chimeric CbhA polypeptide has an amino acid sequence at least 95% identical to SEQ ID NO:6.5. The isolated nucleic acid molecule of claim 1 , wherein the chimeric CbhA polypeptide has the amino acid sequence of SEQ ID NO:6.6. The isolated nucleic acid molecule of claim 1 , further comprising a promoter operably linked to the nucleic acid molecule.7. The isolated nucleic acid molecule of claim 6 , wherein the promoter allows expression of the nucleic acid in a bacterial host cell.8. An expression vector comprising the nucleic acid molecule of .9. A host cell that expresses a recombinant polypeptide encoded by the nucleic acid molecule of .10E. coli. The host cell of claim 9 , wherein the cell is an cell.11. An isolated chimeric CbhA polypeptide encoded by the nucleic acid molecule of .12Clostridium thermocellumCaldicellulosiruptor bescii. An isolated chimeric CbhA polypeptide comprising domains from CbhA and Ce1A polypeptides.13Caldicellulosiruptor bescii. The isolated chimeric CbhA polypeptide of claim 12 , wherein the chimeric CbhA ...

Cellobiohydrolase variants and polynucleotides encoding same

The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. 1. An isolated variant of a parent cellobiohydrolase II , comprising a substitution at a position corresponding to position 464 of residues 18-481 of SEQ ID NO: 2 , wherein the variant has cellobiohydrolase II activity and is selected from the group consisting of:(a) a variant having at least 90% sequence identity to residues 18-481 of SEQ ID NO: 2;(b) a variant encoded by a polynucleotide that hybridizes under high stringency conditions with nucleotides 52-1443 of SEQ ID NO: 1, (ii) the genomic DNA sequence of nucleotides 52-1443 of SEQ ID NO: 1, or (iii) the full-length complementary strand of (i) or (ii); and(c) a variant encoded by a polynucleotide having at least 90% sequence identity to nucleotides 52-1443 of SEQ ID NO: 1 or the genomic DNA sequence thereof.2. The variant of claim 1 , which comprises a substitution at a position corresponding to position 464 of the mature polypeptide of SEQ ID NO: 2 with Ala claim 1 , Arg claim 1 , Asn claim 1 , Asp claim 1 , Cys claim 1 , Gln claim 1 , Glu claim 1 , Gly claim 1 , His claim 1 , Ile claim 1 , Leu claim 1 , Lys claim 1 , Met claim 1 , Phe claim 1 , Pro claim 1 , Ser claim 1 , Thr claim 1 , Trp claim 1 , or Tyr or comprises a substitution at position 464 of the mature polypeptide of SEQ ID NO: 2 with Ala claim 1 , Arg claim 1 , Asn claim 1 , Cys claim 1 , Gln claim 1 , Glu claim 1 , Gly claim 1 , His claim 1 , Ile claim 1 , Leu claim 1 , Lys claim 1 , Met claim 1 , Phe claim 1 , Pro claim 1 , Ser claim 1 , Thr claim 1 , Trp claim 1 , or Tyr.3. The variant of claim 2 , wherein the substitution is Gln.4. The variant of claim 1 , wherein the variant has at least 95% sequence identity to residues 18-481 of SEQ ID NO: 2.5. The variant of claim 1 , ...

FREE ENZYME AND CELLULOSOME PREPARATIONS FOR CELLULOSE HYDROLYSIS

Disclosed herein are combinations of free fungal enzymes and cellulosomes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using the combinations are also disclosed. 1. A method for degrading cellulose or lignocellulosic biomass , comprising contacting a cellulose containing material or lignocellulosic biomass with an enzyme cocktail comprising at least one fungal cellulase and at least one high molecular weight (HMW) cellulosome complex.2Clostridium.. The method of claim 1 , wherein the at least one cellulosome complex is from a bacterium of the genus3C. thermocellum.. The method of claim 2 , wherein the bacterium is4. The method of claim 1 , wherein the at least one fungal cellulase comprises a Family 7 cellobiohydrolase.5Hypocrea.. The method of claim 4 , wherein the Family 7 cellobiohydrolase is from a fungus of the genus6H. jecorina.. The method of claim 5 , wherein the fungus is7. The method of claim 6 , wherein the Family 7 cellobiohydrolase is Cel7A.8. The method of claim 4 , wherein the enzyme cocktail further comprises a β-glucosidase.9. The method of claim 4 , wherein the enzyme cocktail further comprises at least one hemicellulase.10. The method of claim 4 , wherein the enzyme cocktail further comprises at least one oxidoreductase.11. The method of claim 1 , wherein the at least one fungal cellulase comprises CTec2.12. The method of claim 1 , wherein the contacting is carried out at a temperature of between 50-60° C.13. The method of claim 1 , wherein the contacting is carried out at a temperature of 50° C.14. An enzyme cocktail comprising at least one HMW cellulosome complex and a Family 7 cellobiohydrolase.15C. thermocellum.. The enzyme cocktail of claim 14 , wherein the at least one HMW cellulosome complex is from16Hypocrea jecorina.. The enzyme cocktail of claim 14 , wherein the Family 7 cellobiohydrolase is from17. The enzyme cocktail of claim 14 , wherein the Family 7 cellobiohydrolase ...

Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having cellobiohydrolase activity , selected from the group consisting of:(a) a polypeptide having at least 84%, e.g., at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 2, or a polypeptide having at least 81%, e.g., at least 82%, at least 83%, at least 84%, at least 85%, at least 87%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 4;(b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, or medium stringency conditions, or medium-high stringency conditions, or high stringency conditions, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, or SEQ ID NO: 3, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, or SEQ ID NO: 3; or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2, or SEQ ID NO: 4 comprising a substitution, deletion, and/or ...

Proteins for the Treatment of Cellulosic Material

The present invention discloses novel polypeptides and enzyme preparations containing them, which enhance the efficiency of the cellulosic degradation even at elevated temperatures. The polypeptides are produced by recombinant technology, and means for their production are described. The novel polypeptides are useful in processing biomass, and in biofuel, starch, textile, detergent, pulp and paper, food, feed or beverage industries. They may also be used e.g. in cleaning the interior of a dishwashing machine or for biofinishing or biostoning. The novel polypeptides are also useful in animal feed. 1. A GH61 polypeptide comprising an amino acid sequence having at least 75% sequence identity to SEQ ID NO: 23 , at least 78% sequence identity to SEQ ID NO: 24 or at least 79% sequence identity to SEQ ID NO: 25 , or a fragment or variant thereof capable of enhancing hydrolysis of cellulosic material.2. The GH61 polypeptide of claim 1 , wherein the polypeptide has at least 90% identity to SEQ ID NO: 23 claim 1 , SEQ ID NO: 24 or SEQ ID NO: 25 or a fragment thereof capable of enhancing hydrolysis of cellulosic material.3Acremonium thermophilumMelanocarpus albomyces.. The GH61 polypeptide of claim 1 , wherein the polypeptide is obtainable from or4. An isolated polynucleotide selected from the group consisting of:a) a polynucleotide comprising the coding sequence as shown in SEQ ID NO: 20, 21 or 22{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'b) a polynucleotide encoding a polypeptide of ;'}c) a polynucleotide encoding a fragment of a polypeptide encoded by a polynucleotide of a) or b), wherein said fragment is capable of enhancing hydrolysis of cellulosic material; andd) a polynucleotide comprising a nucleotide sequence which is degenerate to the nucleotide sequence of a polynucleotide sequence of a) or b);or the complementary strand of such a polynucleotide.5. The polynucleotide of claim 4 , comprising a gene included in a microorganism having accession number selected ...

Mutant cells for protein secretion and lignocellulose degradation

The present disclosure provides mutant cells for the secretion of proteins and for the degradation of lignocellulosic biomass. Methods for the use of these cells are also provided. Specifically, the utility of combined genetic deletions of β-glucosidases and the catabolite repressor gene creA/cre-1 for protein secretion in fungal and yeast cells is disclosed.

Endoglucanases for Treatment of Cellulosic Material

The present invention relates to production of fermentable sugars from lignocellulosic material by enzymatic conversion. The fermentable sugars are useful e.g. in the production of bioethanol. Novel polypeptides having endoglucanase activity, polynucleotides encoding them and vectors and host cells containing the polynucleotides are disclosed. A method for treating cellulosic material with the novel endoglucanase as well as uses of the enzymes and enzyme preparations and a method of preparing them are described. 1. A polypeptide having endoglucanase activity and comprising an amino acid sequence having at least 57% sequence identity to EG_A having SEQ ID NO: 7 or at least 58% sequence identity to EG_B having SEQ ID NO: 8 , or a fragment or variant thereof having endoglucanase activity.2Acremonium thermophilumA. thermophilum. The polypeptide of claim 1 , wherein said polypeptide is obtainable from claim 1 , preferably from CBS 116240.3Trichoderma reesei.. The polypeptide of claim 1 , wherein said polypeptide is a recombinant fusion protein further comprising a cellulose binding module (CBM) of4T. reeseiT. reesei. The polypeptide of claim 3 , wherein said CBM is a CBM derived from EGI/Cel7B or CHBI/Cel7A.5T. reeseiT. reesei. The polypeptide of claim 3 , wherein the natural CBM of endoglucanase EG_A has been replaced with a CBM of EGI/Cel7B or CHBI/Cel7A and preferably the resulting fusion protein comprises an amino acid sequence having at least 55% sequence identity to SEQ ID NO: 23 or 27 claim 3 , respectively.6T. reeseiT. reesei. The polypeptide of claim 3 , wherein a CBM of EGI/Cel7B or CHBI/Cel7A has been genetically attached to the endoglucanase EG_B and preferably the resulting fusion protein comprises an amino acid sequence having at least 64% sequence identity to SEQ ID NO: 24 or 28 claim 3 , respectively.7. The polypeptide of claim 3 , wherein the fusion protein comprises an amino acid sequence having SEQ ID NO: 23 claim 3 , SEQ ID NO: 24 claim 3 , SEQ ID NO: ...

Compositions for saccharification of cellulosic material

The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof. 1. An enzyme composition , comprising:(I) a polypeptide having cellobiohydrolase I activity selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 6; (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 5, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO: 5, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 5;(II) a polypeptide having cellobiohydrolase II activity selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 18; (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 17, (ii) the cDNA sequence of the mature polypeptide coding sequence of SEQ ID NO: 17, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and (c) a polypeptide encoded by a ...

Polypeptides Having Cellobiohydrolase I Activity and Polynucleotides Encoding Same

The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. 129-. (canceled)30. An isolated polypeptide having cellobiohydrolase I activity , which has at least 80% identity with the sequence of amino acids 1 to 452 of SEQ ID NO:16.31. The polypeptide of claim 30 , which has at least 85% identity with the sequence of amino acids 1 to 452 of SEQ ID NO:16.32. The polypeptide of claim 30 , which has at least 90% identity with the sequence of amino acids 1 to 452 of SEQ ID NO:16.33. The polypeptide of claim 30 , which has at least 95% identity with the sequence of amino acids 1 to 452 of SEQ ID NO:16.34. The polypeptide of claim 30 , which has at least 97% identity with the sequence of amino acids 1 to 452 of SEQ ID NO:16.35. The polypeptide of claim 30 , which has at least 98% identity with the sequence of amino acids 1 to 452 of SEQ ID NO:16.36. The polypeptide of claim 30 , which has at least 99% identity with the sequence of amino acids 1 to 452 of SEQ ID NO:16.37. The polypeptide of claim 30 , comprising the sequence of amino acids 1 to 452 of SEQ ID NO:16.38. The polypeptide of claim 30 , which is a fragment of the sequence of amino acids 1 to 452 of SEQ ID NO:16.39. A detergent composition comprising a surfactant and the polypeptide of .40. A method for producing ethanol from biomass claim 30 , comprising{'claim-ref': {'@idref': 'CLM-00030', 'claim 30'}, '(a) contacting the biomass with the polypeptide of , an endo-1,4-beta-glucanase, and a beta-D-glucosidase to produce sugar; and'}(b) fermenting the sugar to produce ethanol. This application is a divisional of U.S. application Ser. No. 13/681,490 filed on Nov. 20, 2012, now allowed, which is a divisional of U.S. application Ser. No ...

Polypeptides Having Cellobiohydrolase I Activity and Polynucleotides Encoding Same

The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. 129.-. (canceled)30. An isolated polypeptide having cellobiohydrolase I activity , which has at least 80% identity with the sequence of amino acids 1 to 525 of SEQ ID NO:60.31. The polypeptide of claim 30 , which has at least 85% identity with the sequence of amino acids 1 to 525 of SEQ ID NO:60.32. The polypeptide of claim 30 , which has at least 90% identity with the sequence of amino acids 1 to 525 of SEQ ID NO:60.33. The polypeptide of claim 30 , which has at least 95% identity with the sequence of amino acids 1 to 525 of SEQ ID NO:60.34. The polypeptide of claim 30 , which has at least 97% identity with the sequence of amino acids 1 to 525 of SEQ ID NO:60.35. The polypeptide of claim 30 , which has at least 98% identity with the sequence of amino acids 1 to 525 of SEQ ID NO:60.36. The polypeptide of claim 30 , which has at least 99% identity with the sequence of amino acids 1 to 525 of SEQ ID NO:60.37. The polypeptide of claim 30 , comprising the sequence of amino acids 1 to 525 of SEQ ID NO:60.38. The polypeptide of claim 30 , which is a fragment of the sequence of amino acids 1 to 525 of SEQ ID NO:60.39. A detergent composition comprising a surfactant and the polypeptide of .40. A method for producing ethanol from biomass claim 30 , comprising{'claim-ref': {'@idref': 'CLM-00030', 'claim 30'}, '(a) contacting the biomass with the polypeptide of , an endo-1,4-beta-glucanase, and a beta-D-glucosidase to produce sugar; and'}(b) fermenting the sugar to produce ethanol. This application is a divisional of U.S. application Ser. No. 13/681,490 filed on Nov. 20, 2012, now allowed, which is a divisional of U.S. application Ser. ...

SURFACTANT TOLERANT CELLULASE AND METHOD FOR MODIFICATION THEREOF

A method for suppressing a reduction in an endoglucanase activity in the presence of a surfactant, characterized by modifying a protein having the endoglucanase activity in which the N-terminus is an amino acid other than pyroglutamic acid, to a protein having the N-terminus of pyroglutamic acid, is disclosed. Further, a modified protein having an endoglucanase activity wherein the N-terminal amino acid is converted into pyroglutamic acid by an amino acid modification, a polynucleotide encoding the protein, an expression vector comprising the polynucleotide, a host cell transformed with the expression vector, and a process for producing the protein by cultivating the host cell, are disclosed. 1. A polynucleotide encoding a protein selected from the group consisting of:(a) a protein comprising the amino acid sequence of SEQ ID NO: 2, 4, 38, or 40, wherein the N-terminal amino acid is pyroglutamic acid; and(b) a protein comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2, 4, 38, or 40, and having an endoglucanase activity, wherein the N-terminal amino acid is pyroglutamic acid.2. A polynucleotide selected from the group consisting of:(a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1, 3, 37, or 39; and(c) a polynucleotide hybridizing under stringent conditions to a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 1, 3, 37, or 39, and encoding a protein having an endoglucanase activity.3. An expression vector comprising the polynucleotide according to .4. A host cell transformed with the expression vector according to .5. The host cell according to claim 4 , wherein the host cell is a yeast or filamentous fungus.6HumicolaTrichoderma.. The host cell according to claim 5 , the filamentous fungus is a microorganism belonging to genus or7Humicola insolensTrichoderma viride.. The host cell according to claim 6 , the filamentous fungus is or8. A process for producing a protein ...

Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having cellobiohydrolase activity, catalytic domains, and cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains, and cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, or cellulose binding domains. 1. An isolated polypeptide having cellobiohydrolase activity , selected from the group consisting of:(a) a polypeptide having at least 85%, e.g., at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 85%, e.g., at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has cellobiohydrolase activity.2E. coliE. coli. The polypeptide of claim 1 , which is encoded by a polynucleotide which is identical to the polynucleotide contained in plasmid pAJ227 which is contained in NRRL B-50474 or which is identical to the polypeptide encoded by the polynucleotide contained in ...

Novel Glycoside Hydrolases from Thermophlic Fungi

The present invention relates to isolated polypeptides having cellulolytic activity or hemicellulolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having cellobiohydrolase activity , selected from the group consisting of:(a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4; at least 90% sequence identity to the polypeptide of SEQ ID NO: 6; at least 91% sequence identity to the polypeptide of SEQ ID NO: 8; or at least 99% sequence identity to the polypeptide of SEQ ID NO: 10;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with (i) the polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, or SEQ ID NO: 9, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the polypeptide coding sequence of SEQ ID NO: 1 or SEQ ID NO: 3, or the cDNA sequences thereof; at least 90% sequence identity to the polypeptide coding sequence of SEQ ID NO: 5 or the cDNA sequence thereof; at least 91% sequence identity to the polypeptide coding sequence of SEQ ID NO: 7 or the cDNA sequences thereof; or at least 99% sequence identity to the polypeptide coding sequence of SEQ ID NO: 9 or the cDNA sequence thereof;(d) a variant comprising the polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, or SEQ ID NO: 10 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has cellobiohydrolase activity.25-. (canceled)6. An isolated polynucleotide encoding the polypeptide of .7. A recombinant host cell comprising the ...

CELLULASE COMPOSITIONS AND METHODS OF USING THE SAME FOR IMPROVED CONVERSION OF LIGNOCELLULOSIC BIOMASS INTO FERMENTABLE SUGARS

The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having β-glucosidase activity, methods for hydrolyzing biomass material, and methods for improving the stability and saccharification efficacy of a composition comprising such β-glucosidase polypeptides and/or activity. 2. The isolated polypeptide of claim 1 , comprising an amino acid sequence that has at least about 80% identity to SEQ ID NO:135 or at least about 90% identity to SEQ ID NO:135.3. (canceled)4. The isolated polypeptide of claim 1 , comprising the N-terminal sequence derived from the first β-glucosidase and the C-terminal sequence derived from the second β-glucosidase claim 1 , wherein the first β-glucosidase and the second β-glucosidase are different from each other.5. The isolated polypeptide of claim 1 , wherein the N-terminal sequence and the C-terminal sequences are not directly connected claim 1 , but are functionally connected via a linker domain.6. The isolated polypeptide of claim 5 , wherein the N-terminal sequence claim 5 , the C-terminal sequence claim 5 , or the linker domain comprises a loop region sequence of 3 claim 5 , 4 claim 5 , 5 claim 5 , 6 claim 5 , 7 claim 5 , 8 claim 5 , 9 claim 5 , 10 claim 5 , or 11 amino acid residues in length claim 5 , comprising an amino acid sequence of SEQ ID NO:171 or 172.7. The isolated polypeptide of claim 1 , which has improved stability as compared to the first β-glucosidase or to the second β-glucosidase claim 1 , optionally wherein the improved stability is an increased resistance to proteolytic cleavage under storage conditions or production conditions.8. (canceled)9. The isolated polypeptide of claim 4 , wherein:(a) the N-terminal sequence comprises an amino acid sequence that has at least 90% sequence identity to a sequence of the same length of SEQ ID NO:54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78 or 79, wherein the C-terminal sequence comprises a sequence ...

Targeted perhydrolases

Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.

Multifunctional Cellulase And Hemicellulase

A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed. 1. A multifunctional polypeptide capable of hydrolyzing a cellulosic material , xylan , and mannan , comprising{'i': 'Clostridium thermocellum', 'sub': '—', 'the catalytic core (cc) of Cthe0797 (CelE),'}{'i': 'Clostridium thermocellum', 'a cellulose-specific carbohydrate-binding module (CBM), wherein the CBM is CBM3 of the cellulosome anchoring protein cohesion region (CipA) of (CBM3a), and'}a linker region interposed between the catalytic domain and the cellulose-specific carbohydrate binding module.2. The polypeptide of claim 1 , wherein the linker region is a 5-150 amino acid sequence.3. The polypeptide of claim 1 , wherein the linker region is a 15-40 amino acid sequence.4. The polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence of the protein CelEcc_CBM3a (SEQ ID NO:6).5. A polypeptide composition for increasing the rate and the extent of fiber digestion for a mammal comprising the polypeptide of .6. A method of hydrolyzing a substrate comprising a cellulosic material claim 1 , xylan claim 1 , and mannan claim 1 , comprising the step of contacting a substrate comprising a cellulosic material claim 1 , xylan claim 1 , and mannan with an effective amount of the multifunctional polypeptide of claim 1 , whereby the cellulosic material claim 1 , xylan claim 1 , and mannan in the substrate are at least partially hydrolyzed.7. The method of claim 6 , wherein the cellulosic material is selected from the group consisting of filter paper claim 6 , crystalline ...

Cellulose Binding Domain Variants and Polynucleotides Encoding Same

The present invention relates to cellobiohydrolase variants and cellulose binding domain variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. 1. An isolated cellobiohydrolase variant , comprising a substitution at one or more positions corresponding to positions 482 , 484 , and 507 of the mature polypeptide of SEQ ID NO: 2 , wherein the variant has cellobiohydrolase activity and the variant has at least 60% , e.g. , at least 65% , at least 70% , at least 75% , at least 80% , at least 81% , at least 82% , at least 83% , at least 84% , at least 85% , at least 86% , at least 87% , at least 88% , at least 89% , at least 90% , at least 91% , at least 92% , at least 93% , at least 94% , at least 95% , at least 96% , at least 97% , at least 98% , or at least 99% , but less than 100% , sequence identity to the mature polypeptide of SEQ ID NO: 2 , SEQ ID NO: 38 , SEQ ID NO: 40 , SEQ ID NO: 42 , SEQ ID NO: 44 , SEQ ID NO: 46 , or SEQ ID NO: 48.2. The variant of claim 1 , which is a variant of a parent cellobiohydrolase selected from the group consisting of:(a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 42, SEQ ID NO: 44, SEQ ID NO: 46, or SEQ ID NO: 48;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, or SEQ ID NO: 47, (ii) the genomic DNA or cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 60% identity to the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, or SEQ ID NO: 47 or the ...

Methods For Enhancing The Degradation Or Conversion Of Cellulosic Material

The present invention relates to methods for degrading or converting a cellulosic material and for producing a substance from a cellulosic material. 1. A method for degrading or converting a cellulosic material , comprising: treating the cellulosic material with an enzyme composition in the presence of a polypeptide having cellulolytic enhancing activity selected from the group consisting of:(a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has cellulolytic enhancing activity.2. The method of claim 1 , wherein the polypeptide comprises or consists of SEQ ID NO: 2 or the mature polypeptide thereof claim 1 , or a fragment thereof having cellulolytic enhancing activity.3. The method of claim 1 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase claim 1 , a hemicellulase claim 1 , an expansin claim 1 , an esterase claim 1 , a laccase claim 1 , a ligninolytic enzyme claim 1 , a pectinase claim 1 , a peroxidase claim 1 , a protease claim 1 , and a swollenin.4. The method of claim 1 , further comprising recovering the degraded cellulosic material.5. The method of claim 4 , wherein the degraded cellulosic material is a sugar.6. A method for producing a fermentation product claim 4 , comprising: (a) saccharifying a cellulosic material with an enzyme ...

Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 129-. (canceled)30. An isolated polypeptide having endoglucanase activity , selected from the group consisting of:(a) a polypeptide having at least 76% to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2, comprising a substitution, deletion, and/or insertion at one or more positions, having at least 76% to the mature polypeptide of SEQ ID NO: 2; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has endoglucanase activity.31. The polypeptide of claim 30 , having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2.32. The polypeptide of claim 30 , having at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2.33. The polypeptide of claim 30 , having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2.34. The polypeptide of claim 30 , having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.35. The polypeptide of claim 30 , which is encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1 claim 30 , (ii) the cDNA sequence thereof claim 30 , or (iii) the full-length complement of (i) or (ii).36. The polypeptide of claim 30 , comprising or ...

Polypeptides Having Cellulolytic Enhancing Activity And Nucleic Acids Encoding Same

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Polypeptides having endoglucanase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having endoglucanase activity , selected from the group consisting of:(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 4;(b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence that hybridizes under at least very high stringency conditions with (i) SEQ ID NO: 3, (ii) a cDNA sequence of SEQ ID NO: 3, or (iii) a full-length complement of (i) or (ii); and(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 3 or a cDNA sequence thereof.2. The polypeptide of claim 1 , comprising SEQ ID NO: 4; or a fragment thereof having endoglucanase activity.3. A nucleic acid construct comprising an isolated polynucleotide comprising a nucleotide sequence that encodes the polypeptide of operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host.4. A recombinant expression vector comprising an isolated polynucleotide comprising a nucleotide sequence that encodes the polypeptide of .5. An isolated recombinant host cell comprising the nucleic acid construct of .6Penicillium brasilianum. A method of producing the polypeptide of claim 1 , comprising: (a) cultivating an isolated cell under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.7. A method of producing the polypeptide of claim 1 , comprising: (a) cultivating a host cell comprising a nucleic acid construct comprising a nucleotide sequence encoding the polypeptide under conditions conducive for production of the polypeptide; and (b) ...

ALTERING ENZYME BALANCE THROUGH FERMENTATION CONDITIONS

This present disclosure relates to methods for improved production of proteins from a cell culture, particularly to culture components and conditions that can preferentially increase the expression of proteins produced from genes under the control of xylanase gene promoter sequences. The improved methods can be used for the production of enzyme compositions with enhanced xylanase and hem icellulolytic activity. 124-. (canceled)25. A mixed saccharide composition produced by:a) mixing a glucose solution with a transglycosylating enzyme to give an enzyme-glucose mixture;b) incubating the enzyme-glucose mixture at an elevated temperature of 50° C. to 75° C. for a time sufficient to give a processed glucose mixture comprising at least one oligosaccharide;c) mixing the processed glucose mixture with a pentose to yield the mixed saccharide composition,wherein the transglycosylating enzyme is capable of processing the glucose in the glucose mixture into at least one oligosaccharide, which, when mixed with the pentose of c), is capable of enhancing hemicellulase production without significantly reducing cellulase production by filamentous fungi.26. An enzyme composition produced by:a) mixing a glucose solution with a transglycosylating enzyme to give an enzyme-glucose mixture;b) incubating the enzyme-glucose mixture at an elevated temperature of 50° C. to 75° C. for a time sufficient to give a processed glucose mixture comprising at least one oligosaccharide;c) mixing the processed glucose mixture with a pentose to yield a mixed saccharide composition;d) exposing a filamentous fungi to the mixed saccharide composition under conditions conducive to protein expression to generate the enzyme composition,wherein the transglycosylating enzyme is capable of processing the glucose in the glucose mixture into at least one oligosaccharide, which, when mixed with the pentose of c), is capable of enhancing hemicellulase production without significantly reducing cellulase production by ...

Fungal Endoglucanases, Their Production and Use

Novel fungal endoglucanases Cel5 and Cel12 are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry, or in hydrolysis of lignocellulosic material for, e.g. bioethanol production. 1. A fungal endoglucanase polypeptide , which belongs to glycosyl hydrolase family 12 , and which comprises an amino acid sequence having at least at least 83% sequence identity to SEQ ID NO: 68 , or a cellulase active fragment thereof.2Trichoderma.. The endoglucanase polypeptide of claim 1 , which is derived from3. The endoglucanase polypeptide of having at least 90% claim 1 , sequence identity to SEQ ID NO: 68 claim 1 , or a cellulase active fragment thereof.4. The endoglucanase polypeptide of claim 3 , having the amino acid sequence of SEQ ID NO: 68.5. An isolated polynucleotide selected from the group consisting of:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a) a nucleotide sequence having SEQ ID NO: 67, or a sequence encoding the endoglucanse polypeptide of ,'}b) a complementary strand of a), orc) a sequence that is degenerate as a result of the genetic code to anyone of the sequences of a) or b).6E. coli. The polynucleotide of claim 5 , which is similar to a polynucleotide carried by DSM 19899.7. An expression vector claim 5 , comprising a polynucleotide of .8. A host cell comprising the expression vector of .9. A method for the production of an endoglucanase polypeptide of comprising the steps of transforming a host cell with an expression vector encoding said polypeptide claim 1 , and culturing said host cell under conditions enabling expression of said polypeptide claim 1 , and optionally recovering and purifying said polypeptide.10. An enzyme preparation comprising the endoglucanase ...

HETEROLOGOUS EXPRESSION OF FUNGAL CELLOBIOHYDROLASE 2 GENES IN YEAST

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms , and sp. in host cells, such as the yeast . The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems. 169-. (canceled)70. A polynucleotide comprising a nucleic acid which encodes a cellobiohydrolase , or domains , variants or a functional fragments thereof , wherein said nucleic acid is codon-optimized for expression in an heterologous yeast host cell and wherein the cellobiohydrolase has an amino acid sequence at least about 80% of the SEQ ID NO: 11 , SEQ ID NO: 12 , SEQ ID NO: 13 , SEQ ID NO: 14 or SEQ ID NO: 15.71. The polynucleotide of claim 70 , wherein the domains of the cellobiohydrolase is a cellobiohydrolase signal peptide.72. The polynucleotide of claim 71 , wherein the signal peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 23 claim 71 , SEQ ID NO: 25 claim 71 , SEQ ID NO: 28 claim 71 , SEQ ID NO: 31 claim 71 , and SEQ ID NO: 34.73. The polynucleotide of claim 70 , wherein the domain of the cellobiohydrolase is a cellobiohydrolase cellulose-binding module (CBM).74. The polynucleotide of claim 73 , wherein the CBM comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 26 claim 73 , SEQ ID NO: 29 claim 73 , SEQ ID NO: 32 claim 73 , SEQ ID NO: 35 claim 73 , and SEQ ID NO: 36.75. The polynucleotide of claim 70 , wherein the domains of the cellobiohydrolase is a GH family 6 domain.76. The polynucleotide of claim 75 , wherein the GH family 6 domain comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 22 claim 75 , SEQ ID NO: 24 claim 75 , SEQ ID NO: 27 claim 75 , SEQ ID NO: 30 claim 75 , ...

GENES ENCODING CELLULASE

Polynucleotide sequences are provided encoding a thermostable cellulase and directing its increased expression are provided, and the use of the thermostable cellulase in hydraulic fracturing methods and the treatment of flowback fluids. 1. A nucleotide sequence of SEQ ID NO:1. This application is a divisional of U.S. application Ser. No. 14/389,339, which is the United States National Phase filing of International Application No.: PCT/US2013/030527, filed Mar. 12, 2013, which designated the United States, was published in English and claims priority of U.S. Provisional Applications 61/618,610, filed Mar. 30, 2012 and 61/704,368, filed Sep. 21, 2012. The contents of the aforementioned applications are hereby expressly incorporated by reference in their entireties.Polynucleotide sequences encoding a cellulase are provided. In particular, the polynucleotide sequences may provide increased expression of a specific, thermostable, thermotolerant, pressure stable enzyme such as a cellulase.This application is being filed electronically via the USPTO EFS-WEB server, as authorized and set forth in MPEP § 502.05 and this electronic filing includes an electronically submitted sequence listing; the entire content of this sequence listing is hereby incorporated by reference into the specification of this application. The sequence listing is identified on the electronically filed ASCII (.txt) text file as follows:O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of naturally-occurring enzymes that hydrolyze the glycosidic bond between two or more carbohydrates or between a carbohydrate and a non-carbohydrate moiety. The International Union of Biochemistry and Molecular Biology (IUBMB) enzyme nomenclature of glycosyl hydrolases (or glycosylases) is based principally on their substrate specificity and occasionally on their molecular mechanism (Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (NC-IUBMB), Accessed Oct. 24, 2011).IUBMB ...

Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having cellulolytic enhancing activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains. 120-. (canceled)21. A nucleic acid construct , comprising a polynucleotide encoding a GH61 polypeptide having cellulolytic enhancing activity , wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the GH61 polypeptide in a recombinant host cell , and wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of:(a) a GH61 polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 4;(b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO: 3 or the cDNA sequence of the mature polypeptide coding sequence of SEQ ID NO: 3, wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, for 12 to 24 hours, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.; and(c) a GH61 polypeptide encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3 or the cDNA sequence of the mature polypeptide coding sequence of SEQ ID NO: 3.22. The nucleic acid construct of claim 21 , wherein the GH61 polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 4.23. The nucleic acid construct of claim 21 , wherein the GH61 ...

Cellulolytic enzyme compositions and uses thereof

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions. 123-. (canceled)24Aspergillus fumigatusAspergillus fumigatusAspergillus fumigatusPenicillium. An enzyme composition comprising: (a) an cellobiohydrolase I; (b) an cellobiohydrolase II; (c) an beta-glucosidase or a variant thereof; and (d) a sp. GH61 polypeptide having cellulolytic enhancing activity; or homologs thereof;{'i': 'Aspergillus fumigatus', 'wherein the cellobiohydrolase I or homolog thereof is selected from the group consisting of(i) a cellobiohydrolase I comprising amino acids 27 to 532 of SEQ ID NO: 2;(ii) a cellobiohydrolase I comprising an amino acid sequence having at least 90% sequence identity to amino acids 27 to 532 of SEQ ID NO: 2;(iii) a cellobiohydrolase I encoded by a polynucleotide comprising a nucleotide sequence having at least 90% sequence identity to nucleotides 79 to 1596 of SEQ ID NO: 1; and(iv) a cellobiohydrolase I encoded by a polynucleotide that hybridizes under high stringency conditions with the full-length complement of nucleotides 79 to 1596 of SEQ ID NO: 1, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;{'i': 'Aspergillus fumigatus', 'wherein the cellobiohydrolase II or homolog thereof is selected from the group consisting of(i) a cellobiohydrolase II comprising amino acids 20 to 454 of SEQ ID NO: 4;(ii) a cellobiohydrolase II comprising an amino acid sequence having at least 90% sequence identity to amino acids 20 to 454 of SEQ ID NO: 4;(iii) a cellobiohydrolase II encoded by a polynucleotide comprising a nucleotide sequence having at least 90% sequence identity to nucleotides 58 to 1700 of SEQ ID NO: 3; and(iv) a cellobiohydrolase II ...

Cellobiohydrolase Variants and Polynucleotides Encoding Same

The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing and using the variants. 1. A cellobiohydrolase variant , comprising a substitution at one or more positions corresponding to positions 112 , 154 , 197 , 228 , 261 , 306 , and 375 of the mature polypeptide of SEQ ID NO: 2 , wherein the variant has cellobiohydrolase activity.2. The variant of claim 1 , which has at least 60% claim 1 , at least 65% claim 1 , at least 70% claim 1 , at least 75% claim 1 , at least 80% claim 1 , at least 81% claim 1 , at least 82% claim 1 , at least 83% claim 1 , at least 84% claim 1 , at least 85% claim 1 , at least 86% claim 1 , at least 87% claim 1 , at least 88% claim 1 , at least 89% claim 1 , at least 90% claim 1 , at least 95% claim 1 , at least 96% claim 1 , at least 97% claim 1 , at least 98% claim 1 , or at least 99% claim 1 , but less than 100% claim 1 , sequence identity to the amino acid sequence of a parent cellobiohydrolase.3. The variant of claim 1 , which is a variant of a parent cellobiohydrolase selected from the group consisting of: (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2 claim 1 , SEQ ID NO: 4 claim 1 , SEQ ID NO: 6 claim 1 , SEQ ID NO: 8 claim 1 , SEQ ID NO: 10 claim 1 , SEQ ID NO: 12 claim 1 , SEQ ID NO: 14 claim 1 , SEQ ID NO: 16 claim 1 , SEQ ID NO: 18 claim 1 , SEQ ID NO: 20 claim 1 , SEQ ID NO: 22 claim 1 , SEQ ID NO: 24 claim 1 , SEQ ID NO: 26 claim 1 , SEQ ID NO: 28 claim 1 , SEQ ID NO: 30 claim 1 , SEQ ID NO: 32 claim 1 , SEQ ID NO: 34 claim 1 , SEQ ID NO: 36 claim 1 , SEQ ID NO: 38 claim 1 , SEQ ID NO: 40 claim 1 , SEQ ID NO: 42 claim 1 , SEQ ID NO: 44 claim 1 , SEQ ID NO: 46 claim 1 , SEQ ID NO: 48 claim 1 , SEQ ID NO: 50 claim 1 , SEQ ID NO: 52 claim 1 , SEQ ID NO: 54 claim 1 , SEQ ID NO: 56 claim 1 , SEQ ID ...

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. An isolated polypeptide having cellulolytic enhancing activity , selected from the group consisting of:(a) a polypeptide comprising an amino acid sequence having at least 80% identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii);(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 80% identity to the mature polypeptide coding sequence of SEQ ID NO: 1; and(d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO: 2.2. The polypeptide of claim 1 , comprising or consisting of the amino acid sequence of SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2; or a fragment thereof having cellulolytic enhancing activity.3E. coli. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pGEM-T-GH61 D23Y4 which is contained in DSM 22882.4. An isolated polynucleotide comprising a nucleotide sequence that encodes the polypeptide of .5. A method of producing the polypeptide of claim 1 , comprising: (a) cultivating a cell claim 1 , which in its wild-type form produces the polypeptide claim 1 , under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.6. A method of producing a polypeptide having cellulolytic enhancing activity claim 4 , comprising: (a) cultivating ...

Method for producing washing enzyme having protease resistance

Provided is a method for producing washing enzyme having protease resistance. According to the method, the washing enzyme having resistance to protease is obtained by carrying out fusion expression on a gene of the washing enzyme with the gene of a protease inhibitory peptide, thereby facilitating maintaining the stability of various enzyme components in an enzyme-containing detergent, and improving the use effect of the detergent.

Soft biomass decomposition method

An object of the present invention is to provide a soft biomass decomposition method, a production method for a target substance from soft biomass, and an enzyme or group of enzymes for decomposing soft biomass. Provided is a soft biomass decomposition method, including a step of bringing an enzyme selected from specific exocellulase, endocellulase, and processive endocellulase into contact with soft biomass such as bagasse and rice straw. Also provided is a production method for a target substance from soft biomass by incorporating the soft biomass decomposition method as a step. Further provided is an enzyme or group of enzymes for decomposing soft biomass selected from specific exocellulase, endocellulase, and processive endocellulase.

Polypeptide having endoglucanase activity

The present invention relates to a family 5 glycoside hydrolase variant having endoglucanase activity, polynucleotides encoding the family 5 glycoside hydrolase variant, vectors, host cells comprising the polynucleotides, and methods for using the family 5 glycoside hydrolase variant.

GH61 GLYCOSIDE HYDROLASE PROTEIN VARIANTS AND COFACTORS THAT ENHANCE GH61 ACTIVITY

The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol. 1. A polynucleotide comprising a nucleic acid sequence encoding a GH61 variant protein that is at least about 90% identical to SEQ ID NO:2 or a polynucleotide that hybridizes under stringent hybridization conditions to the polynucleotide and/or a complement of a polynucleotide encoding said GH61 variant protein.2. A polynucleotide sequence encoding a GH61 variant protein , wherein said polynucleotide sequence is at least about 75% , at least about 80% , at least about 85% , at least about 90% , at least about 91% , at least about 92% , at least about 93% , at least about 94% , at least about 95% , at least about 96% , at least about 97% , at least about 98% , or at least about 99% identical to any of SEQ ID NOS:1 , 4 , 7 , and/or 10 , or a polynucleotide that hybridizes under stringent hybridization conditions to the polynucleotide and/or a complement of any of SEQ ID NOS:1 , 4 , 7 , and/or 10.3. A recombinant nucleic acid construct comprising at least one polynucleotide sequence encoding at least one GH61 protein , wherein the polynucleotide is selected from: (a) a polynucleotide that encodes a polypeptide comprising an amino acid sequence having at least about 70% , at least about 75% , at least about 80% , at least about 85% , at least about 90% , at least about 91% , at least about 92% , at least about 93% , at least about 94% , at least about 95% , at least about 96% , at least about 97% , at least about 98% , or at least about 99% identity to SEQ ID NO:2 , 3 , 5 , 6 , 8 , and/or 9 , wherein the amino acid sequence comprises at least one ...

Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

COMPOSITIONS FOR ENHANCED ENZYME PRODUCTION

The present invention relates to compositions to induce production of proteins, e.g., enzymes, e.g., amylases or biomass degrading enzymes in a host cell, and methods for increasing the yield of the proteins, e.g., enzymes produced. Such compositions comprise a caramelized sugar product. The methods described herein can also be used to enhance processing of biomass materials, e.g., to produce sugar products. 1. A method for inducing production of a biomass degrading enzyme comprising contacting a microorganism that produces the biomass degrading enzyme with a composition comprising a caramelized sugar product under conditions sufficient for production of a biomass degrading enzyme.2. The method of claim 1 , wherein the microorganism is in a cell culture.3. The method of claim 2 , wherein sugar is added to the cell culture prior to contacting the microorganism with the composition comprising a caramelized sugar product.4. The method of claim 3 , wherein the microorganism is contacted with the composition comprising a caramelized sugar product when the cell culture is substantially free from sugar.5. The method of or claim 3 , wherein the caramelized sugar product is produced by caramelizing glucose claim 3 , xylose claim 3 , maltose claim 3 , lactose claim 3 , or a combination thereof.6. The method of claim 5 , wherein the caramelized sugar product produced by caramelizing saccharified biomass comprises xylose and glucose.7. The method of claim 5 , or claim 5 , wherein the caramelized sugar product comprises oligosaccharides claim 5 , dehydration products of the oligosaccharides claim 5 , hydration products of the oligosaccharides claim 5 , disproportionation products of the oligosaccharides claim 5 , colored aromatic products claim 5 , or any combination thereof.8. The method of claim 7 , wherein the oligosaccharides comprise disaccharides claim 7 , trisaccharides claim 7 , tetrasaccharides claim 7 , pentasacchrides claim 7 , hexasaccharides claim 7 , or a ...

Polypeptides Having Cellobiohydrolase Activity And Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 126-. (canceled)27. A process for degrading a cellulosic material , comprising: treating the cellulosic material with an enzyme composition comprising a polypeptide having cellobiohydrolase activity , wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:(a) a polypeptide having at least 90% sequence identity to the sequence of amino acids 26 to 532 of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with the full-length complement of the sequence of nucleotides 76 to 1596 of SEQ ID NO: 1 or the cDNA sequence thereof, wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, following standard Southern blotting procedures for 12 to 24 hours, followed by washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;(c) a polypeptide fragment of the sequence of amino acids 26 to 532 of SEQ ID NO: 2 that has cellobiohydrolase activity;(d) a polypeptide comprising a catalytic domain having cellobiohydrolase activity, wherein the catalytic domain has at least 90% sequence identity to the sequence of amino acids 26 to 460 of SEQ ID NO: 2; and(e) a polypeptide fragment of the sequence of amino acids 26 to 460 of SEQ ID NO: 2 that has cellobiohydrolase activity.28. The process of claim 27 , wherein the polypeptide having cellobiohydrolase activity comprises the sequence of amino acids 26 to 532 of SEQ ID NO: 2.29. The process of claim 27 , wherein the catalytic domain comprises the sequence of amino acids 26 to 460 of SEQ ID ...

Method for Restoring Sexual Reproduction in the Fungus Trichoderma Reesei

The present invention relates to a method for restoring sexual reproduction between two sterile, female strains of using a helper strain ΔMAT, said helper strain being a fertile female strain of in which the sexual-type locus MAT has been eliminated. 1Trichoderma reesei,. A method for restoring sexual reproduction between two sterile female strains of comprising the following steps:{'i': 'Trichoderma reesei', 'a) incubation in a suitable medium of a ΔMAT helper strain, said strain being a fertile female strain of wherein the locus of the MAT mating type has been knocked out,'}{'i': 'Trichoderma reesei', 'b) a first watering of said ΔMAT helper strain with conidia of a first strain of a first mating type,'}{'i': 'Trichoderma reesei', 'c) a second watering of said ΔMAT helper strain resulting from step b) with conidia of a second strain of a second mating type.'}2Trichoderma reeseiTrichoderma reesei. The method for restoring sexual reproduction between two sterile female strains of as claimed in claim 1 , wherein the first mating type of the first strain is MAT1-1.3Trichoderma reeseiTrichoderma reesei. The method for restoring sexual reproduction between two sterile female strains of as claimed in claim 1 , wherein the second mating type of the second strain is MAT1-2.4Trichoderma reeseiTrichoderma reesei. The method for restoring sexual reproduction between two sterile female strains of as claimed in claim 1 , wherein the strain is the QM6a strain or a strain derived from the QM6a strain.5Trichoderma reesei. The method for restoring sexual reproduction between two sterile female strains of as claimed in claim 1 , wherein step a) of incubating claim 1 , in a suitable medium claim 1 , said ΔMAT helper strain lasts at least 2 days.6Trichoderma reesei. The method for restoring sexual reproduction between two sterile female strains of as claimed in claim 1 , wherein step a) of incubating claim 1 , in a suitable medium claim 1 , said ΔMAT helper strain is an incubation in ...

BIOPOLYMER, PROCESS FOR PRODUCING A BIOPOLYMER, PROCESS FOR PRODUCING PAPER, PROCESS FOR PRODUCING CELLULOSE, USE OF A BIOPOLYMER PRODUCT

The present invention is directed to the production of a biopolymer, which may be applied both as an additive in the papermaking process, aiming at improving the physical and mechanical properties thereof in the process for producing cellulose, facilitating the drying process of the same, while conferring the same properties mentioned above for the production of paper, when it is processed. The present invention further relates to the process for producing said biopolymer, the papermaking process, the process for producing cellulose, the use of said biopolymer and the product comprising said biopolymer. 1. A biopolymer characterized in that it is derived from a cationized cellulose fiber of maize , wheat , sorghum , rice , barley , sugarcane or combinations thereof and in that it has:a. a size of from 50 to 1000 μm in length and 5 to 80 μm in width; andb. a degree of cationization ranging from 0.005 to 0.1%.2. The biopolymer of claim 1 , characterized in that it has a degree of cationization ranging from 0.010 to 0.05%.3. The biopolymer of claim 1 , wherein it is in an aqueous solution comprising:i. a total solids content ranging from 1 to 50% by weight of the biopolymer by the total volume of the solution;ii. viscosity ranging from 10 to 90,000 cP at 50° C.;iii. density ranging from 0.900 to 1.300 g/ml at 5% solids by weight at 25° C. by the dry weight of the composition;iv. electric conductivity ranging from 3,000.0 to 25,000.0 μS/cm at 5% solids by weight at 25° C. by the dry weight of the composition; andv. pH ranging from 2.0 to 14.0, preferably 5.0 to 12.0.4. The biopolymer of claim 1 , wherein it is for use as paper or cellulose strength enhancer.5. A process for producing the biopolymer of claim 1 , comprising the steps of:A. digesting the fiber of maize, wheat, sorghum, rice, barley, sugarcane bagasse or combinations thereof through a basic pH medium ranging from 10 to 14 and at a temperature ranging from 40 to 120° C., more preferably 50 to 80° C.; andB. ...

Cellulase suitable for use in detergent compositions

The present invention relates to a novel cellulase, the use of the novel cellulase in various applications such as detergent compositions and a composition comprising the novel cellulase. 1. Cellulase comprising the catalytic domain motif [STA]-T-R-Y-[FYW]-D-x(5)-[CA] and a carbohydrate binding domain with a sequence identity of at least 80% to SEQ ID NO: 3 or a carbohydrate binding domain comprising the tag motif V-[PSC]-[DQEN]-S-G-G-P-G-P-G-P-G-P-G-P.2. Cellulase according to claim 1 , wherein the catalytic domain motif is T-T-R-Y-[FYW]-D-x(5)-[CA].3. Cellulase according to any of or claim 1 , wherein the catalytic domain motif is T-T-R-Y-W-D-x(5)-C.4. Cellulase according to any of to claim 1 , wherein the catalytic domain motif is T-T-R-Y-W-D-C-C-K-P-S-C.5. Cellulase according to any of the preceding claims claim 1 , comprising a sequence with at least 80% sequence identity to SEQ ID NO: 1.6. Cellulase according to any of the preceding claims claim 1 , further comprising a linker.7. Cellulase according to claim 6 , wherein the linker has a sequence identity of at least 80% to SEQ NO: 5.8. Cellulase according to claim 6 , wherein the linker has a sequence identity of at least 80% to SEQ NO: 7.9. Cellulase according to any of or claim 6 , wherein the linker comprises an amino acid sequence of [AGSVT](5 claim 6 ,65).10. Cellulase according to claim 8 , wherein the linker comprises an amino acid sequence of ([SG]-P)(5 claim 8 ,10).11. Use of the cellulase as defined in any of to in textile claim 8 , feed claim 8 , food claim 8 , biomass hydrolysis claim 8 , plant oil refining claim 8 , detergent and pulp and paper processing applications.12. Use of the cellulase according to as a biofinishing agent such as a depilling agent or a defuzzing agent; a biostoning agent; a fabric care agent such as a color clarification agent claim 11 , an anti greying agent claim 11 , a softness agent claim 11 , an antipilling agent; a laundry agent such as an an anti-redeposition agent ...

ß-GLUCOSIDASE

The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from cellulolyticus, a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided. 1. A β-glucosidase , comprising a β-glucosidase catalytic domain which comprises:{'b': '1', '(A) a polypeptide comprising an amino acid sequence represented by SEQ ID NO. ,'}(B) a polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having β-glucosidase activity, or(C) a polypeptide comprising an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having β-glucosidase activity.2. The β-glucosidase according to claim 1 , which has β-glucosidase activity at pH 3.0 to pH 5.5 and a temperature of 30° C. to 45° C. that uses p-Nitrophenyl β--glucopyranoside as a substrate.3. A polynucleotide claim 1 , comprising a region that encodes a β-glucosidase catalytic domain which comprises:(a) a base sequence that encodes a polypeptide comprising an amino acid sequence represented by SEQ ID NO: 1,(b) a base sequence that encodes a polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence ...

ß-GLUCOSIDASE

The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from , a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided. 1. A β-glucosidase , comprising a β-glucosidase catalytic domain which comprises:(A) a polypeptide comprising an amino acid sequence represented by SEQ ID NO. 1,(B) a polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having β-glucosidase activity, or(C) a polypeptide comprising an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having β-glucosidase activity.2. The β-glucosidase according to claim 1 , which has β-glucosidase activity at pH 3.0 to pH 5.5 and a temperature of 30° C. to 60° C. that uses p-Nitrophenyl 3-D-glucopyranoside as a substrate.3. A polynucleotide claim 1 , comprising a region that encodes a β-glucosidase catalytic domain which comprises:(a) a base sequence that encodes a polypeptide comprising an amino acid sequence represented by SEQ ID NO: 1,(b) a base sequence that encodes a polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and ...

BETA-GLUCOSIDASE

The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from , a polynucleotide encoding the β-glucosidase, an expression vector for expressing the β-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the β-glucosidase can be provided. 1. A β-glucosidase , comprising a β-glucosidase catalytic domain which comprises:(A) a polypeptide comprising an amino acid sequence represented by SEQ ID NO. 1,(B) a polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having β-glucosidase activity, or(C) a polypeptide comprising an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having β-glucosidase activity.2. The β-glucosidase according to claim 1 , which has β-glucosidase activity at pH 3.0 to pH 5.5 and a temperature of 30° C. to 60° C. that uses p-Nitrophenyl β-D-glucopyranoside as a substrate.3. A polynucleotide claim 1 , comprising a region that encodes a β-glucosidase catalytic domain which comprises:(a) a base sequence that encodes a polypeptide comprising an amino acid sequence represented by SEQ ID NO: 1,(b) a base sequence that encodes a polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and ...

Methods For Producing Multiple Recombinant Polypeptides In A Filamentous Fungal Host Cell

The present invention relates to methods for constructing a filamentous fungal strain for production of multiple recombinant polypeptides having biological activity. The present invention also relates to methods for producing multiple recombinant polypeptides having biological activity in a filamentous fungal strain. The present invention also relates to filamentous fungal strains expressing multiple recombinant polypeptides having biological activity. 124-. (canceled)25. A filamentous fungal strain , comprising:(a) an endogenous first gene replaced by targeted integration with a first tandem construct comprising (i) a homologous 5′ region of the first gene, a homologous flanking region thereof, or a combination thereof, (ii) one or more first selectable markers, (iii) a first polynucleotide encoding a first polypeptide having biological activity operably linked to a first promoter and a first terminator, (iv) a second polynucleotide encoding a second polypeptide having biological activity operably linked to a second promoter and a second terminator, and (v) a homologous 3′ region of the first gene, a homologous flanking region thereof, or a combination thereof; and(b) an endogenous second gene replaced by targeted integration with a second tandem construct comprising (i) a homologous 5′ region of the second gene, a homologous flanking region thereof, or a combination thereof, (ii) one or more second selectable markers, (iii) a third polynucleotide encoding a third polypeptide having biological activity operably linked to a third promoter and a third terminator, (iv) a fourth polynucleotide encoding a fourth polypeptide having biological activity operably linked to a fourth promoter and a fourth terminator, and (v) a homologous 3′ region of the second gene, a homologous flanking region thereof, or a combination thereof.26. The filamentous fungal strain of claim 25 , wherein the first gene is a cellobiohydrolase I gene.27. The filamentous fungal strain of claim 26 , ...

Methods for Using Polypeptides Having Cellobiohydrolase Activity

The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

BACTERIA AND ENZYMES PRODUCED THEREFROM AND METHODS OF USING SAME

A bacteria referred to here as 6A-1 is provided, compositions thereof and processes for use of the bacteria, spores, cells, extracts and enzymes. The compositions which comprise the bacteria, spores, cells, extracts and/or enzymes are capable of degrading polysaccharides. Such compositions are capable of degrading cellulose, including plant-produced cellulose, microcrystalline cellulose and carboxymethyl cellulose. The bacteria produces at least two cellulose-degrading protein fractions. Cellulose degrading activity continues across pH2 to pH13. 125.-. (canceled)26Bacillus subtilis. A method of degrading a composition comprising at least one polysaccharide , said method comprising combining a composition comprising 6A-1 (6A-1) , reference culture comprising said 6A-1 having been deposited at ATCC under deposit number PTA-125135 , or cells of said 6A-1 , or spores produced by said 6A-1 , or at least one polysaccharide-degrading enzyme extracted from said 6A-1 , or combination thereof , wherein said 6A-1 or cells or spores are filtered , dried , freeze dried or ground , or have added thereto at least one excipient , carrier or diluent to , and contacting said composition comprising said 6A-1 or cells or spores or at least one polysaccharide-degrading enzyme or combination thereof with said composition comprising said at least one polysaccharide and degrading said at least one polysaccharide.27. The method of claim 26 , wherein said composition comprising said 6A-1 or cells or spores or at least one polysaccharide-degrading enzyme or combination thereof comprises at least three cellulose-degrading protein fractions produced by said 6A-1 claim 26 , said protein fractions capable of degrading crystalline cellulose claim 26 , carboxymethyl cellulose and unmodified cellulose.28. The method of claim 26 , wherein composition comprising said 6A-1 or cells or spores or at least one polysaccharide-degrading enzyme or combination thereof is capable of degrading protein at a pH ...

PROCESSES FOR RECOVERING PRODUCTS FROM A CORN FERMENTATION MASH

Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product. 1. A process for recovering products from a fermentation mash , comprising:processing a ground corn product to produce a fermentation mash comprising ethanol;separating at least a portion of the ethanol from the fermentation mash to produce a whole stillage; andseparating the whole stillage to produce a fiber rich product and a filtrate, wherein the fiber rich product has an ethanol potential of at least 90 gallons of ethanol per 1,000 kilograms of the fiber rich product.2. The process of claim 1 , wherein the ethanol potential is 90 gallons of ethanol to about 160 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.3. The process of claim 1 , wherein the ethanol potential is 90 gallons of ethanol to about 145 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.4. The process of claim 1 , wherein the ethanol potential is about 100 gallons of ethanol to about 160 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.5. The process of claim 1 , wherein the ethanol potential is about 100 gallons of ethanol to about 150 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.6. The process of claim 1 , wherein the ethanol potential is about 100 gallons of ethanol to about 145 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.7. The process of claim 1 , ...

RASAMSONIA TRANSFORMANTS

A method for carrying out recombination at a target locus in a cell, which method comprises: providing two or more nucleic acids which, when taken together, comprise: (a) sequences capable of homologous recombination with sequences flanking the target locus; (b) two or more site-specific recombination sites; (c) a sequence encoding a recombinase which recognizes the site-specific recombination sites; and (d) a sequence encoding a marker, wherein the two or more nucleic acids are capable of homologous recombination with each other so as to give rise to a single nucleic acid, and wherein at least two of the two or more nucleic acids each comprise a sequence encoding a non-functional portion of the marker; and recombining in a cell the said two or more nucleic acids with each other and with the sequences flanking the target locus so that a contiguous nucleic acid sequence encoding a functional marker and the sequence encoding the recombinase are inserted at the target locus, said marker-encoding and/or recombinase-encoding sequence being flanked by at least two site-specific recombination sites and the said site-specific recombination sites being flanked by the sequences capable of homologous recombination with sequences flanking the target locus, thereby to carry out recombination at a target locus in a cell. The method may be used to generate marker free cells. 1Rasamsonia. A method for carrying out recombination at a target locus in a cell , which method comprises:providing two or more nucleic acids which, when taken together, comprise: (a) sequences capable of homologous recombination with sequences flanking the target locus; (b) two or more site-specific recombination sites; (c) a sequence encoding a recombinase which recognizes the site-specific recombination sites; and (d) a sequence encoding a marker,wherein the two or more nucleic acids are capable of homologous recombination with each other so as to give rise to a single nucleic acid, andwherein at least two ...

PROCESSES FOR RECOVERING PRODUCTS FROM A CORN FERMENTATION MASH

Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product. 1. A process for recovering products from a fermentation mash , comprising:processing a ground corn product to produce a fermentation mash comprising ethanol;separating at least a portion of the ethanol from the fermentation mash to produce a whole stillage;separating the whole stillage to produce a fiber rich product and a filtrate;hydrolyzing the fiber rich product to produce a saccharification mash; andprocessing the saccharification mash to produce additional ethanol and a stillage protein product.2. The process of claim 1 , wherein the stillage protein product comprises about 15 wt % to about 35 wt % of yeast claim 1 , based on a dry weight of the stillage protein product.3. The process of claim 1 , wherein the stillage protein product comprises about 40 wt % to about 80 wt % of protein claim 1 , about 3 wt % to about 20 wt % of fat claim 1 , about 1 wt % to about 4 wt % of ash claim 1 , about 2 wt % to about 30 wt % of neutral detergent fibers claim 1 , about 1 wt % to about 15 wt % of acid detergent fibers claim 1 , and about 15 wt % to about 35 wt % of yeast claim 1 , based on a dry weight of the stillage protein product.4. The process of claim 1 , wherein the stillage protein product comprises about 47 wt % to about 57 wt % of protein claim 1 , about 3 wt % to about 5 wt % of fat claim 1 , about 1 wt % to about 3 wt % of ash claim 1 , about 4 wt ...

Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Quinone Compound And Uses Thereof

The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a quinone compound. The present invention also relates to methods of using the compositions.

Novel process for enzymatic acrylamide reduction in food products

The present invention relates to a novel enzyme composition comprising asparaginase and at least one hydrolysing enzyme, the use of such composition to reduce acrylamide levels in food products and a method to produce food products involving at least one heating step, comprising adding: a) asparaginase and b) at least one hydrolyzing enzyme to an intermediate form of said food product in said production process whereby the asparaginase and at least one hydrolyzing enzyme are added prior to said heating step in an amount that is effective in reducing the level of acrylamide of the food product in comparison to a food product whereto no asparaginase and hydrolyzing enzyme were added.