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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 3958. Отображено 100.

12-01-2012 дата публикации

Biomass hydrothermal decomposition system and saccharide-solution production method using biomass material

Номер: US20120009642A1

Автор: Hideo Suzuki, Yoshio Kuromi, Yoshitaka Kimura

Принадлежит: Mitsubishi Heavy Industries Ltd

A biomass hydrothermal decomposition system includes a hydrothermal decomposition unit 17 that transports the fed biomass material from a lower side to an upper side in an apparatus body 13 by screw means 14, feeds pressurized hot water 15 from an upper side different from a feed position of the biomass material 11 into the apparatus body 13, which is pressurized hot water to be discharged, so as to separate a lignin component and a hemicellulose component from the biomass material; a biomass solid discharging unit 18 that discharges a biomass solid 20 from the upper side of the apparatus body 13; and a slurrying vessel 21 communicating with the biomass solid discharging unit 18, into which water 19 is injected and the discharged biomass solid 20 is added to obtain a slurried biomass solid are provided.

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Номер записи: 1

03-05-2012 дата публикации

Production Of Pure Lignin From Lignocellulosic Biomass

Номер: US20120108798A1

Автор: David A. Hogsett, John Basdsley, Kevin S. Wenger, Michael Ladisch

Принадлежит: Mascoma Corp

The present invention is directed to a process of producing substantially pure lignin from lignocellulosic biomass, which comprises: pre-treating a lignocellulosic feedstock to produce a reactive lignin-carbohydrate mixture; biologically-reacting the carbohydrates in the mixture, separating remaining solids from the liquid fermentation products, and drying the resulting solids to yield a substantially pure lignin product. Optionally, the lignin product may be washed and subjected to a second hydrolysis step. Optionally, the lignin product may be further processed by hydrotreating and/or pyrolysis in order to yield desirable products such as fuel additives.

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Номер записи: 2

24-05-2012 дата публикации

Continuously fed biomass pretreatment process for a packed bed reactor

Номер: US20120125548A1

Автор: Jeffrey David Cohen

Принадлежит: EI Du Pont de Nemours and Co

Biomass pretreatment using anhydrous ammonia is effective in a static reactor vessel when the ammonia can penetrate through the biomass particles or pieces in vapor state, and when biomass is continuously fed and moved through the reactor. To achieve this condition, total system moisture content is kept below 40 weight % based on total mass in the system. The pretreated biomass product is effectively saccharified to produce fermentable sugars for biocatalyst production of a product.

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Номер записи: 3

05-07-2012 дата публикации

De-fatted soy production process and value added by-products from de-fatted soy flour

Номер: US20120171740A1

Автор: John E. Prevost, Neal A. Hammond

Принадлежит: Individual

An improved process for producing de-fatted soy utilizing a de-fatted soy flour and for producing value added by-products from de-fatted soy flour wherein soybeans are de-hulled and the de-hulled stream ground to a flour consistency. The ground soy flour is mixed with water and other additives to produce a vitamin and mineral enriched stream that is then filtered to various value added by-products. In a preferred embodiment the vitamin and mineral enriched stream is filtered through a 0.1-1.0 micron membrane to produce a de-fatted soy product stream and a fatted soy product stream. The fatted soy product stream can be dried to produce dry, less than 12% water by weight, product B for use in cosmetics and pharmaceutical products. The de-fatted soy product can be filtered through reverse osmosis (RO) filtration unit to obtain a vitamin and mineral enriched product stream that can be dried to powder form and used as a food supplement additive I. If desired the full fatted soy flour or the de-fatted soy product stream can be combined with whole stillage from an ethanol process and used in producing various other value added products.

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Номер записи: 4

04-10-2012 дата публикации

Biomass shock pretreatment

Номер: US20120252070A1

Автор: John J. Dunkleman, Mark A. Deimund, Mark T. Holtzapple, Matthew Falls, Maxine Jones Madison, Rocio Sierra Ramirez

Принадлежит: TEXAS A&M UNIVERSITY SYSTEM

Methods and apparatus for treating biomass that may include introducing a biomass to a chamber; exposing the biomass in the chamber to a shock event to produce a shocked biomass; and transferring the shocked biomass from the chamber. In some aspects, the method may include pretreating the biomass with a chemical before introducing the biomass to the chamber and/or after transferring shocked biomass from the chamber.

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Номер записи: 5

08-11-2012 дата публикации

Enhanced soluble c5 saccharide yields

Номер: US20120282655A1

Автор: Phillip R. Gibbs

Принадлежит: Renmatix Inc

Methods are disclosed for increasing the level of soluble C 5 saccharides produced from lignocellulosic biomass comprising acidifying fractionated lignocellulosic biomass to prevent the recondensation of soluble C 5 saccharides, including C 5 oligosaccharides and xylose and arabinose monomers, to insoluble higher molecular weight C 5 oligosaccharides.

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Номер записи: 6

13-12-2012 дата публикации

Saccharide-solution producing apparatus, fermentation system, saccharide-solution producing method, and fermentation method

Номер: US20120315677A1

Автор: Gaku Kondo, Hideo Suzuki, Michio Nishiyama, Minoru Genta, Seiichi Terakura

Принадлежит: Mitsubishi Heavy Industries Mechatronics Systems Ltd

A saccharide-solution producing apparatus 11 A according to the present invention is a saccharide-solution producing apparatus for producing a saccharide solution 22 derived from a carbohydrate-based material 21 , and includes a saccharide-solution controlling unit 15 A that controls the saccharide solution derived from the carbohydrate-based material 21 , a cellulosic biomass saccharifying unit 16 that saccharifies hydrothermally treated biomass obtained by hydrothermally decomposing a cellulosic biomass material 35 that contains a lignin component and a hemicellulose component, and produces a diluted saccharide solution 37 , and a diluted-saccharide-solution supply pipe L 11 that mixes the diluted saccharide solution 37 produced by the cellulosic biomass saccharifying unit 16 into the saccharide-solution controlling unit 15 A. With this configuration, it is possible to improve production efficiency of the saccharide solution 22 and to realize cost reduction.

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Номер записи: 7

17-01-2013 дата публикации

Process for the production of alcohols and/or solvents from papermaking pulps with recycling of non-hydrolyzed vegetation

Номер: US20130017585A1

Автор: Caroline Aymard, Marcel Ropars

Принадлежит: IFP Energies Nouvelles IFPEN

This invention describes a process for the production of alcohols and/or solvents from cellulosic or lignocellulosic biomass that comprises at least the following stages: a) Alkaline chemical pretreatment based on sodium sulfate of a cellulosic or lignocellulosic substrate; b) Washing of the pretreated substrate; c) Enzymatic hydrolysis of the substrate that is pretreated and washed using cellulolytic and/or hemicellulolytic enzymes that produce a hydrolyzate and a water-insoluble residue; d) Microorganism fermentation of the hydrolyzate that is obtained from stage c) and production of a fermentation must that contains at least one alcohol and/or solvent; e) Separation/purification of alcohol and/or solvent, and f) Separation of a cake that contains the insoluble residue, in which at least a portion of the cake that is obtained in stage f) is recycled upstream from the pretreatment stage a) and/or upstream from the washing stage b).

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Номер записи: 8

24-01-2013 дата публикации

Cooling and processing materials

Номер: US20130023020A1

Автор: Marshall Medoff

Принадлежит: Xyleco Inc

Systems and methods for cooling and processing materials are disclosed.

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Номер записи: 9

14-03-2013 дата публикации

System for the treatment of biomass

Номер: US20130065289A1

Автор: David Charles Carlson

Принадлежит: Poet Research Inc

A system for treating biomass for the production of ethanol is disclosed. A biorefinery for producing a fermentation product from biomass is disclosed. The biorefinery comprises a system for preparing the biomass into prepared biomass and a system for pre-treating the biomass into pre-treated biomass. The biorefinery comprises a separator, a first treatment system, a second treatment system, and a fermentation system. A method for producing a fermentation product from biomass is disclosed.

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Номер записи: 10

28-03-2013 дата публикации

Pretreatment Method for Producing Water-Soluble Sugars From Lignocellulosic Material

Номер: US20130078695A1

Автор: Jaakko Palola, Janne Sandqvist, Paivi Rousu

Принадлежит: CHEMPOLIS OY

The invention relates to manufacturing hydrolyzable cellulose and further, if desired, sugars from lignocellulosic material by means of formic and performic acid treatment.

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Номер записи: 11

06-06-2013 дата публикации

Method for dilute acid pretreatment of lignocellulosic feedstocks

Номер: US20130143285A1

Автор: Daphne Wahnon, Jeffrey S. Tolan, Steven Cardile

Принадлежит: Iogen Energy Corp

The present invention relates to a process for the conversion of a lignocellulosic feedstock involving acid pretreatment. The process comprises the steps of treating the lignocellulosic feedstock with alkali at a pH of between about 8.0 and about 12.0 so as to dissolve acetyl groups present on said lignocellulosic feedstock, while converting less than about 10% of the xylan present in the lignocellulosic feedstock to xylose and less than about 10% of the cellulose to glucose, thereby producing an alkali conditioned feedstock. The alkali conditioned feedstock is then pretreated at a temperature of about 160° C. to about 250° C., at a pH of about 0.5 to about 2.5 for about 0.5 to about 10 minutes so as to hydrolyze about 80 to 100% of the xylan and about 3 to about 15% of the cellulose to produce an acid pretreated feedstock comprising cellulose. The cellulose in the pretreated feedstock can be hydrolyzed to glucose with cellulase and the glucose can be fermented to produce a fermentation product.

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Номер записи: 12

01-08-2013 дата публикации

Processing biomass

Номер: US20130196386A1

Автор: Marshall Medoff

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 13

12-09-2013 дата публикации

Methods for Producing a Fermentation Product from Lignocellulose-Containing Material

Номер: US20130236933A1

Автор: Feng Xu, Haiyu Ren, HONGZHI HUANG, Ye Chen, Yun Wang

Принадлежит: China Petroleum and Chemical Corp, Cofco Corp, Novozymes AS

The present invention provides a method for producing a fermentation product from lignocellulose-containing material, a method for converting lignocellulose-containing material into a hydrolyzate comprising mono- and oligo-saccharides, and a method for treating lignocellulose-containing material, all of which comprise the step of mixing an acid pre-treated lignocellulose-containing material and an alkaline pre-treated lignocellulose-containing material. The present invention further provides a fermentation product made according to the method for producing a fermentation product.

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Номер записи: 14

19-09-2013 дата публикации

Processes and apparatus for producing fermentable sugars and low-ash biomass for combustion at reduced emissions

Номер: US20130244290A1

Автор: Theodora Retsina, Vesa Pylkkanen

Принадлежит: API Intellectual Property Holdings LLC

This invention provides processes and apparatus to convert biomass, including wood and agricultural residues, into low-ash biomass pellets for combustion, alone or in combination with another solid fuel. Some embodiments provide processes for producing hemicellulosic sugars and low-ash biomass from cellulosic biomass, comprising providing an aqueous extraction solution with acetic acid; extracting the feedstock to produce an extract liquor containing soluble ash, hemicellulosic oligomers, acetic acid, dissolved lignin, and cellulose-rich solids; dewatering and drying the cellulose-rich, lignin-rich solids to produce a low-ash biomass; hydrolyzing the hemicellulosic oligomers to produce fermentable hemicellulosic sugars, wherein additional acetic acid is generated; removing a vapor stream comprising vaporized acetic acid from the extract; recycling the vapor or its condensate to provide some starting acetic acid for the extraction solution; and recovering fermentable hemicellulosic sugars. The disclosed processes can produce clean power from biomass. Co-products include fermentation products such as ethanol, fertilizers, and lignin.

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Номер записи: 15

17-10-2013 дата публикации

Processing biomass and petroleum containing materials

Номер: US20130273612A1

Автор: Marshall Medoff

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 16

31-10-2013 дата публикации

Processing Biomass Containing Materials

Номер: US20130288307A1

Автор: Marshall Medoff

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 17

07-11-2013 дата публикации

Processing biomass

Номер: US20130295624A1

Автор: Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 18

28-11-2013 дата публикации

Pre-treated biomass having enhanced enzyme accessibility

Номер: US20130313472A1

Автор: Francesco Cherchi, Paolo Torre, Piero Ottonello, Simone Ferrero

Принадлежит: Beta Renewables Spa

This invention is to a biomass composition of 5 and 6 carbon sugars, lignin, and cellulose which has been steam exploded and the composition has a very high enzyme accessibility at 24 hours for hydrolysis.

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Номер записи: 19

26-12-2013 дата публикации

Methods using peracids for controlling corn ethanol fermentation process infection and yield loss

Номер: US20130344556A1

Автор: Jay Kummet, Peter J. Fernholz

Принадлежит: ECOLAB USA INC

A process for the use of peracid compositions to eliminate and/or control the growth of undesirable bacteria, including contaminating bacteria, in the fermentation production of alcohol is disclosed. Beneficially, the peracid compositions and methods of use of the same do not interfere or inhibit the growth or replication of yeast and have low or no adverse environmental impact.

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Номер записи: 20

26-12-2013 дата публикации

Processing biomass

Номер: US20130344586A1

Автор: Harrison Medoff, Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation.

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Номер записи: 21

02-01-2014 дата публикации

Processing biomass

Номер: US20140004570A1

Автор: Marshall Medoff, Michael W. Finn, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Provided herein are methods of increasing the efficiency of biomass saccharification. In particular, the methods include ways of avoiding feedback inhibition during the production of useful products.

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Номер записи: 22

06-02-2014 дата публикации

Process for acetic acid removal from pretreated biomass

Номер: US20140034253A1

Автор: Paolo Corbellani, Simone Ferrero

Принадлежит: Beta Renewables Spa

The process for treating a ligno-cellulosic biomass feed stream comprised of solids, C5's, C6's, lignin, and water comprises the steps of: pretreating the ligno-cellulosic biomass feed stream by contacting the ligno-cellulosic biomass with water in the temperature range of 40 to 210 ° C. to create a pre-treated ligno-celluosic biomass comprised of a pre-treatment ligno-cellulosic biomass liquid comprised of suspended solids, C5's, C6's, and acetic acid, wherein the ratio of the C6's to C5's is less than 0.8 to 1.0, and a pre-treated ligno-cellulosic biomass solids; separating a portion of the pre-treatment ligno cellulosic biomass liquid from the pre-treated ligno-cellulosic biomass feed stream; separating a portion of the suspended solids from the pre-treatment ligno-cellulosic biomass liquid using filters, centrifuge or combination thereof, to create a clarified liquid stream, and nano filtering a portion of the clarified liquid stream to create a nano-filtered permeate stream comprised of acetic acid and water and a nano-filtered retentate stream comprised of C5's, C6's, acetic acid and water, wherein the ratio of acetic acid to the total amount of C5's, C6's in the clarified liquid stream is greater than the ratio of the acetic acid to the total amount of C5's, C6's in the nano-filtered retentate.

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Номер записи: 23

06-02-2014 дата публикации

Processing biomass

Номер: US20140038251A1

Автор: Marshall Medoff, Michael W. Finn, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Fructose, e.g., fructose derived from a cellulosic or lignocellulosic material, is use, e.g., fermented to produce a product, e.g., a solvent.

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Номер записи: 24

27-02-2014 дата публикации

Method for processing vegetable biomass

Номер: US20140053827A1

Автор: Celia Maria Araujo Galvao, Dionisio Fabiano Pegoretti, Dionisio Morelli Filho, Henrique Macedo Baudel, Jaime Fingerut, Jose Augusto Travassos Rios Tome, Liliane Pires Andrade, Osawaldo Godoy Neto

Принадлежит: CTC-Cento de Tecnologia Canavieira SA

The present invention relates to an energy-efficient process for the treatment of plant biomass, particularly sugar cane, for the production of carbohydrates and ethanol, using physico-chemical and extraction techniques, as well as very simple milling configurations, thereby minimizing energy consumption during extraction of the cane juice. The biomass treated and obtained through this process, when subjected to a fermentation process for the production of ethanol, increases the yield of the process in comparison with that of traditional sugar cane. It can also be used for the production of enzymes, animal feedstuffs, and other useful products.

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Номер записи: 25

03-04-2014 дата публикации

Polypeptides Having Cellulolytic Enhancing Activity And Nucleic Acids Encoding Same

Номер: US20140093940A1

Автор: Alfredo Lopez de Leon, Edward Re, Elena Vlasenko, Elizabeth Zaretsky, Keith Mcfarland, Kimberly Brown, Paul Harris

Принадлежит: Novozymes Inc

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

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Номер записи: 26

07-01-2016 дата публикации

Biomass processing using ionic liquids

Номер: US20160002358A1

Автор: Kurtis G. Knapp, Rodrigo E. TEIXEIRA

Принадлежит: HYRAX ENERGY Inc

Without limitation, the disclosure provides processes for (a) dissolving biomass in ionic liquids, (b) deconstructing cellulose, hemicellulose and/or lignin into derivatives including fermentable sugars, (c) separating the biomass derivatives from the ionic liquid, and (d) converting the biomass derivatives to useful fuels or chemicals, either dissolved within or separated from the ionic liquid. It should be understood that processes described herein can be used in isolation or in combination with each other.

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Номер записи: 27

07-01-2016 дата публикации

METHODS FOR DETOXIFYING A LIGNOCELLULOSIC HYDROLYSATE

Номер: US20160002359A1

Автор: Kustedjo Karen, Sato Yukiko, SLUPSKA MALGORZATA, Wong Kelvin

Принадлежит:

The present disclosure relates to methods for detoxifying a hydrolysate obtained from a lignocellulosic biomass and methods of producing ethanol from the detoxified hydrolysate. The present methods provide detoxified hydrolysates in which the quantity of compounds that are deleterious to fermenting microorganisms are substantially reduced relative to the starting hydrolysate and in which the amount of fermentable sugars loss is minimal. 1. A method of reducing the toxicity of a lignocellulosic hydrolysate towards a fermenting organism , or for reducing at least a portion of one inhibitor to a fermenting organism from a lignocellulosic hydrolysate , comprising the step of mixing a starting lignocellulosic hydrolysate solution , said starting lignocellulosic hydrolysate solution comprising a mixture of fermentable sugars , furan aldehydes and aliphatic acids , with a magnesium base selected from magnesium hydroxide , magnesium carbonate and magnesium oxide for a period of time and under conditions that result in the formation of a detoxified hydrolysate solution comprising at least 90% of the total fermentable sugars present in the starting lignocellulosic hydrolysate solution and no greater than 40% of furan aldehydes present in the starting lignocellulosic hydrolysate solution , thereby reducing the toxicity of the lignocellulosic hydrolysate.2. The method of claim 1 , wherein the starting hydrolysate solution is prepared by hydrolyzing a lignocellulosic biomass.3. The method of claim 2 , wherein the lignocellulosic biomass is selected from Napier grass claim 2 , energy cane claim 2 , sorghum claim 2 , giant reed claim 2 , sugar beet claim 2 , switchgrass claim 2 , bagasse claim 2 , rice straw claim 2 , miscanthus claim 2 , switchgrass claim 2 , wheat straw claim 2 , wood claim 2 , wood waste claim 2 , paper claim 2 , paper waste claim 2 , agricultural waste claim 2 , municipal waste claim 2 , birchwood claim 2 , oat spelt claim 2 , corn stover claim 2 , eucalyptus ...

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Номер записи: 28

07-01-2016 дата публикации

SOLAR STEAM PROCESSING OF BIOFUEL FEEDSTOCK AND SOLAR DISTILLATION OF BIOFUELS

Номер: US20160002673A1

Автор: Halas Nancy J., Neumann Oara, Nordlander Peter, Urban Alexander

Принадлежит: William Marsh Rice University

A method of producing bioethanol that includes receiving a feedstock solution that includes polysaccharides in a vessel comprising a complex is described. The complex may be copper nanoparticles, copper oxide nanoparticles, nanoshells, nanorods, carbon moieties, encapsulated nanoshells, encapsulated nanoparticles, and/or branched nanostructures. The method also includes applying electromagnetic (EM) radiation to the complex such that the complex absorbs the EM radiation to generate heat. Using the heat generated by the complex, sugar molecules may be extracted from the polysaccharides in the feedstock solution, and fermented. Then, bioethanol may be extracted from the vessel. 1. A method of producing bioethanol , the method comprising:receiving, in a vessel comprising a complex, a feedstock solution comprising polysaccharides, wherein the complex is a least one selected from a group consisting of copper nanoparticles, copper oxide nanoparticles, nanoshells, nanorods, carbon moieties, encapsulated nanoshells, encapsulated nanoparticles, and branched nanostructures;applying electromagnetic (EM) radiation to the complex, wherein the complex absorbs the EM radiation to generate heat;extracting, using the heat generated by the complex, sugar molecules from the polysaccharides in the feedstock solution; andfermenting the sugars molecules to generate bioethanol; andextracting the bioethanol from the vessel.2. The method of claim 1 , wherein extracting the bioethanol from the vessel comprises:condensing, using a condenser, the bioethanol from the vessel; andstoring the bioethanol in a storage tank.3. The method of claim 1 , wherein extracting the bioethanol from the vessel comprises:applying additional EM radiation to the complex, wherein the complex absorbs the additional EM radiation to generate additional heat;transforming, using the additional heat generated by the complex, the bioethanol to a vapor; andextracting the vapor from the vessel.4. The method of claim 1 , ...

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Номер записи: 29

05-01-2017 дата публикации

PROCESSES FOR FERMENTATION OF LIGNOCELLULOSIC GLUCOSE TO ALIPHATIC ALCOHOLS OR ACIDS

Номер: US20170002387A1

Автор: PYLKKANEN Vesa, RETSINA Theodora

Принадлежит:

A process for producing an organic aliphatic product (such as butanol) from lignocellulosic biomass is provided, comprising: (a) fractionating lignocellulosic biomass in the presence of a solvent for lignin, a hydrolysis catalyst, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin; (b) washing the cellulose-rich solids and separating the cellulose-rich solids from the liquor; (c) enzymatically hydrolyzing the cellulose-rich solids to generate a hydrolysate comprising glucose; (d) detoxifying the hydrolysate by neutralizing the hydrolysate, removing insoluble solids, and removing or oxidizing residual hydrolysis catalyst, thereby generating a purified hydrolysate; (e) fermenting the purified hydrolysate using a suitable microorganism to produce a dilute organic aliphatic product, wherein the microorganism is recycled with a membrane; (f) extracting the dilute organic aliphatic product into a water-immiscible extractant, to generate an intermediate material; and (g) distilling the intermediate material to generate a concentrated organic aliphatic product. 1. A process for producing an organic aliphatic product from lignocellulosic biomass , said process comprising:(a) fractionating a feedstock comprising lignocellulosic biomass in the presence of a solvent for lignin, a hydrolysis catalyst, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin;(b) washing said cellulose-rich solids and separating said cellulose-rich solids from said liquor;(c) enzymatically hydrolyzing said cellulose-rich solids to generate a hydrolysate comprising glucose;(d) detoxifying said hydrolysate by neutralizing said hydrolysate, removing insoluble solids, and removing or oxidizing residual hydrolysis catalyst, thereby generating a purified hydrolysate;(e) fermenting said purified hydrolysate using a suitable microorganism in a fermentor to produce a dilute organic aliphatic product, wherein said microorganism is ...

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Номер записи: 30

05-01-2017 дата публикации

Method of preparing seaweed-derived galactose using agarase

Номер: US20170002389A1

Автор: Chae Hwan Hong, Na Kyong Yun, SungWan Jeon

Принадлежит: Hyundai Motor Co

Provided is a method of preparing galactose by enzymatic treatment of a red algae residue. The method of preparing galactose comprises: preparing a residue; treating the residue with an enzyme; concentrating the enzyme treated residue containing sugar mixture, and precipitating and particulating galactose by adding an alcohol to the concentrated sugar mixture. The preparation method according to the present invention provides a technique capable of industrially producing a substantial amount of galactose which is utilized as an important intermediate material in the preparation of biochemical materials.

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Номер записи: 31

02-01-2020 дата публикации

METHOD FOR TREATING LIGNOCELLULOSIC BIOMASS

Номер: US20200002482A1

Автор: Aymard Caroline, KNOSPE Emilia, PEROTTA Larissa, ROUSSET Romain

Принадлежит:

The invention relates to a process for treating a lignocellulosic biomass, with: 2. Process according to claim 1 , characterized in that the continuous filter functions counter-currentwise between the circulation of the solid/liquid mixture to be separated and the extraction/washing fluid.3. Process according to claim 1 , characterized in that the separation of the solid/liquid mixture is performed during step b on a first portion of the pretreated substrate obtained on conclusion of step a claim 1 , in that a second portion of said pretreated substrate is subjected to at least one subsequent treatment step claim 1 , notably hydrolysis claim 1 , and in that the solid phase enriched in solid obtained in step b is also subjected to at least one subsequent treatment step claim 1 , notably the same as those to which the second portion of the pretreated substrate is subjected.4. Process according to claim 1 , characterized in that it comprises a step e of enzymatic hydrolysis of the pretreated substrate ofrived from the pre-treatment step a to obtain a hydrolysate claim 1 , in that the solid/liquid separation b is performed on at least a portion of said hydrolysate.5. Process according to claim 1 , characterized in that the solid/liquid separation b is performed on at least a portion claim 1 , notably all claim 1 , of the pretreated substrate obtained on conclusion of the pretreatment step a to obtain a solid phase enriched in solid claim 1 , and in that it comprises a step e of enzymatic hydrolysis of said solid phase enriched in solid to obtain a hydrolysate.6. Process according to claim 1 , characterized in that the liquid phases enriched in liquid ofrived from the separation step b are sugary liquors claim 1 , and in that said process also comprises a step c of producing enzymes and/or a step d of producing yeasts claim 1 , and in that at least one of said sugary liquors is used for said production of enzymes c or of yeasts d.8. Process according to claim 1 , ...

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Номер записи: 32

04-01-2018 дата публикации

METHOD OF PRODUCING SACCHARIDES HAVING GLUCOSE AS MAJOR COMPONENT

Номер: US20180002730A1

Автор: Taneda Daisuke

Принадлежит:

A method of efficiently producing saccharides having glucose as the major component by inexpensively suppressing the non-productive adsorption of the enzyme to lignin is provided. The method of producing saccharides includes: a first step of preparing a water-soluble protein by adding at least any one of an animal protein and a vegetable protein to an aqueous sodium hydroxide solution or an aqueous calcium hydroxide solution to react with each other; a second step of adding the water-soluble protein to a slurry including a biomass; and a third step of producing saccharides having glucose as a major component by adding a degrading enzyme to the slurry for at least any one of a cellulose or a hemicellulose included in the biomass to be degraded by the degrading enzyme simultaneously with addition of the water-soluble protein to the slurry or after addition of the water-soluble protein. 1. A method of producing saccharides comprising:a first step of preparing a water-soluble protein by adding at least any one of an animal protein and a vegetable protein to an aqueous sodium hydroxide solution or an aqueous calcium hydroxide solution to react with each other;a second step of adding the water-soluble protein to a slurry including a biomass; anda third step of producing saccharides having glucose as a major component by adding a degrading enzyme to the slurry for at least any one of a cellulose or a hemicellulose included in the biomass to be degraded by the degrading enzyme simultaneously with addition of the water-soluble protein to the slurry or after addition of the water-soluble protein to the slurry.2. The method of producing saccharides according to claim 1 , wherein a concentration of the aqueous sodium hydroxide solution or a concentration of the aqueous calcium hydroxide solution is 0.05 mol/L to 1 mol/L.3. The method of producing saccharides according to claim 1 , wherein an addition amount of the at least any one of the animal protein and the vegetable protein ...

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Номер записи: 33

02-01-2020 дата публикации

PROCESS FOR RECOVERING HIGHER SUGAR FROM BIOMASS

Номер: US20200002778A1

Автор: GUPTA Ravi Prakash, Kumar Ravindra, PURI Suresh Kumar, RAMAKUMAR Sankara Sri Venkata, SATLEWAL Alok, SEMWAL Surbhi

Принадлежит:

An advantageous process for pre-treating lignocellulosic biomass to hydrolysable polysaccharide enriched biomass with reduced amount of inhibitors of catalysis comprising steps of: providing a feedstock comprising cellulosic biomass; chopping of cellulosic biomass to have a cellulosic biomass feed material of uniform size; feeding of uniform sized feed material to a horizontal counter current extraction unit; removing excess of water from the feed material obtained from the extraction unit; soaking the extruded feed material obtained from the extraction unit; optionally removing excess of water from the feed material obtained after acid soaking; steam pre-treating the feed material obtained after removing excess of water from the acid soaked feed material; neutralizing the slurry obtained from the steam pre-treating; and optionally saccharifying the neutralized slurry. 1. A process for pre-treating lignocellulosic biomass to hydrolysable polysaccharide enriched biomass comprising steps of:a) providing a feedstock comprising cellulosic biomass;b) chopping of cellulosic biomass using knife-mill to have a cellulosic biomass feed material of size in the range of 1 mm to 40 mm;c) feeding of feed material obtained from the preceding step b) in to a horizontal counter current extraction unit through one end;d) removing excess of water from the feed material obtained from the preceding step c);e) soaking the feed material obtained from the preceding step d);f) optionally removing excess of water from the feed material obtained from preceding step e);g) steam pre-treating the feed material obtained from the preceding step f);h) neutralizing the slurry obtained from the preceding step g); andi) optionally saccharifying the neutralized slurry obtained from the preceding step h).2. The process as claimed in claim 1 , wherein in step c) a hot water is simultaneously introduced in to a horizontal counter current extraction unit from the other end.3. The process as claimed in ...

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Номер записи: 34

07-01-2021 дата публикации

PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS

Номер: US20210002676A1

Автор: Gierveld Elisabeth Maria, Smits Johannes Petrus

Принадлежит:

The invention relates to a process for the preparation of a sugar and/or fermentation product from lignocellulosic material. 1. A process for preparation of a sugar product from lignocellulosic material , comprising:a) optionally, pretreatment of the lignocellulosic material;b) optionally, washing of the optionally pretreated lignocellulosic material;c) fed-batch addition of the optionally washed and/or optionally pretreated lignocellulosic material to a first container that comprises an enzyme composition comprising at least two cellulases;d) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material in the first container using the enzyme composition comprising at least two cellulases to liquefy the lignocellulosic material;e) addition of the liquefied lignocellulosic material to a second container;f) enzymatic hydrolysis of the liquefied lignocellulosic material in the second container using an enzyme composition comprising at least two cellulases to obtain a sugar product; andg) optionally, recovery of the sugar product.2. A process for preparation of a fermentation product from lignocellulosic material , comprising:a) optionally, pretreatment of the lignocellulosic material;b) optionally, washing of the optionally pretreated lignocellulosic material;c) fed-batch addition of the optionally washed and/or optionally pretreated lignocellulosic material to a first container that comprises an enzyme composition comprising at least two cellulases;d) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material in the first container using the enzyme composition comprising at least two cellulases to liquefy the lignocellulosic material;e) addition of the liquefied lignocellulosic material to a second container;f) enzymatic hydrolysis of the liquefied lignocellulosic material in the second container using an enzyme composition comprising at least two cellulases to obtain a hydrolysed ...

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Номер записи: 35

07-01-2021 дата публикации

Process for obtaining a concentrated protein-rich phase from residues of bioethanol production

Номер: US20210002686A1

Автор: Anne Lamp, Jan Kühling, Julia Pohl, Michael SCHLIMBACH, Oliver Lüdtke, Wolfram Klein

Принадлежит: VERBIO VEREINIGTE BIOENERGIE AG

1) A method for obtaining a concentrated protein-rich phase from residues of bioethanol production. 2.1) Previously, the separation of a protein-rich phase from whole stillage from bioethanol production has been achieved either by the addition of chemicals or by process steps that are complex in terms of equipment and/or energy. 2.2) Whole stillage from bioethanol production is fed to a solid-liquid separation, and the liquid phase (thin stillage) resulting from this is partially returned to the mashing process. This recirculation increases the raw protein content in the process. Part of the thin stillage is diluted and fed to a simple separation process without the addition of chemicals and temperature treatment, with a protein-rich phase being obtained. 2.3) A protein-rich phase is obtained from residues of bioethanol production.

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Номер записи: 36

03-01-2019 дата публикации

METHOD FOR THE SIMULTANEOUS PRODUCTION OF ETHANOL AND A FERMENTED, SOLID PRODUCT

Номер: US20190002929A1

Автор: Ellegard Katrine Hvid, Hansen Ole Kaae, Thomsen Karl Kristian

Принадлежит: Hamlet Protein A/S

The invention relates to a method for the simultaneous production of a fermented, solid product and ethanol comprising the following steps: 1) providing a mixture of milled or flaked or otherwise disintegrated biomass, comprising oligosaccharides and/or polysaccharides and live yeast in a dry matter ratio of from 2:1 to 100:1, and water; 2) fermenting the mixture resulting from step (1) under conditions where the water content in the initial mixture does not exceed 65% by weight, for 1-36 hours at a temperature of about 25-60°C. under anaerobic conditions; 3) incubating the fermented mixture resulting from step (2) for 0.5-240 minutes at a temperature of about 70-150°C.; and 4) separating wet fermented, solid product from the fermented mixture resulting from step (3); further comprising either a) that the fermentation in step (2) is performed in one or more interconnected paddle worm or continuous worm conveyers with inlet means for the fermentation mixture and additives and outlet means for the ferment as well as control means for rotation speed, temperature and pH, or b) that one or more processing aids are added in any of steps (1), (2) and (3) and further comprising a step of 5) separating crude ethanol from the fermented mixture in step (2) by vacuum and/or in step (3) by vacuum or by injection of steam and condensing the surplus stripping steam. The invention further relates to the products of this method as well as uses thereof. 125-. (canceled)26. A fermented , solid protein product obtained from a biomass by a process comprising:(1) providing an initial mixture of a milled or flaked or otherwise disintegrated biomass, comprising proteins and oligosaccharides and/or polysaccharides, and live yeast in a dry matter ratio of from 2:1 to 100:1, and water, wherein the water content in the initial mixture does not exceed 65% by weight of the initial mixture;(2) adding one or more processing aids to the mixture prior to fermenting,(3) fermenting the mixture for 1- ...

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Номер записи: 37

03-01-2019 дата публикации

WET OXIDATION OF BIOMASS

Номер: US20190002930A1

Автор: Foody Brian, Foody Patrick J.

Принадлежит:

A process for producing a transportation fuel from a lignocellulosic feedstock comprising subjecting a stream comprising lignin to a wet oxidation that produces low molecular weight carboxylic acids. These carboxylic acids and/or the corresponding esters are fed to a hydrogenation reaction or gas fermentation wherein they are converted to an alcohol. Heat from the wet oxidation may be supplied to any stage of the process in which heat is introduced. 150.-. (canceled)51. A process for producing ethanol from lignocellulosic feedstock comprising:(i) hydrolyzing at least a portion of the hemicellulose and cellulose in the lignocellulosic feedstock to provide an aqueous mixture comprising insoluble lignin and a soluble sugar;(ii) fermenting the soluble sugar to provide ethanol, said fermenting comprising mixing the soluble sugar with a fermentation microorganism;(iii) separating the insoluble lignin from at least portion of the soluble sugar or the ethanol;(iv) subjecting a mixture comprising the separated insoluble lignin to a wet oxidation to produce at least one of acetic acid, acetate, and carbon dioxide;(v) converting at least a portion of the at least one of acetic acid, acetate, and carbon dioxide produced by the wet oxidation to ethanol;(vi) recovering ethanol produced in (ii) and (v).52. The process according to claim 51 , wherein step (iii) comprises separating the insoluble lignin from at least a portion of the soluble sugar claim 51 , said separating comprising conducting a solid-liquid separation on the aqueous mixture produced in (i) to produce lignin solids and a liquid comprising the soluble sugar claim 51 , and wherein the mixture subjected to the wet oxidation comprises the lignin solids.53. The process according to claim 51 , wherein step (iii) comprises separating the insoluble lignin from at least a portion of the ethanol claim 51 , said separating comprising distilling an aqueous mixture comprising the ethanol and the insoluble lignin claim 51 , and ...

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Номер записи: 38

01-01-2015 дата публикации

METHOD OF PRODUCING SUGAR SOLUTION, SUGAR SOLUTION, AND METHOD OF PRODUCING ETHANOL

Номер: US20150004647A1

Автор: Kishimoto Junpei, Kurihara Hiroyuki, Minamino Atsushi, Nishida Takanori, Niwa Masahiro, Shindo Sho, Yamada Katsushige

Принадлежит:

A method of producing a sugar solution includes a pretreatment step of treating cellulosic biomass with a treatment agent containing ammonia to obtain an ammonia-treated product, an ammonia-treated sugar solution preparation step of enzymatically saccharifying the ammonia-treated product to obtain an ammonia-treated sugar solution, and a purified sugar solution preparation step of removing coumaramide and/or ferulamide in the ammonia-treated sugar solution by purification to obtain a purified sugar solution having a concentration of coumaramide and/or ferulamide of 10 to 1,100 ppm. 1. A method of producing a sugar solution comprising:a pretreatment step of treating cellulosic biomass with a treatment agent containing ammonia to obtain an ammonia-treated product;an ammonia-treated sugar solution preparation step of enzymatically saccharifying the ammonia-treated product to obtain an ammonia-treated sugar solution; anda purified sugar solution preparation step of removing coumaramide and/or ferulamide in the ammonia-treated sugar solution by purification to obtain a purified sugar solution having a concentration of coumaramide and/or ferulamide of 10 to 1,100 ppm.2. The method according to claim 1 , wherein the cellulosic biomass contains herbaceous biomass.3. The method according to claim 1 , wherein in a purification treatment of the ammonia-treated sugar solution claim 1 , a nano-filtration membrane is used.4. The method according to claim 1 , wherein the ammonia-treated product is enzymatically saccharified with a solution having a concentration of a solid matter of the ammonia-treated product of 1 to 10% by mass during enzymatic saccharification of the ammonia-treated product.5. A sugar solution obtained by a method of producing a sugar solution claim 1 , the method comprising:a pretreatment step of treating cellulosic biomass with a treatment agent containing ammonia to obtain an ammonia-treated product;an ammonia-treated sugar solution preparation step of ...

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Номер записи: 39

03-01-2019 дата публикации

METHOD FOR SAVING ENERGY IN PAPER PRODUCTION

Номер: US20190003120A1

Автор: Azhayev Alexey, Birikh Klara

Принадлежит:

The present invention is in the field of paper production, more in particular it relates to the process of wood pulping. It provides useful biological methods and compounds for reducing the energy requirements of the production of wood pulp. It describes a method for reducing the energy requirement of a thermo-mechanical pulping (TMP) process wherein cellulose fibers are recovered from a biomass comprising lignocellulosic material, wherein the lignocellulosic material is treated with a CotA laccase before recovering the cellulose from the lignocellulosic material. 120.-. (canceled).21. A vector comprising:a nucleic acid molecule encoding a protein having laccase activity and having a primary amino acid sequence having at least 99% sequence identity with the sequence of COT1 (SEQ ID NO:1) or COT2 (SEQ ID NO:2); anda polynucleotide heterologous to the nucleic acid molecule encoding a protein having laccase activity.22. The vector of claim 21 , wherein the protein having laccase activity is COT1 (SEQ ID NO:1) or COT2 (SEQ ID NO:2).23. Wood chips comprising the vector of . This application is a divisional of U.S. patent application Ser. No. 14/478,527, filed Sep. 18, 2015, pending, which application is a national phase entry under 35 U.S.C. § 371 of International Patent Application PCT/EP2013/055866, filed Mar. 20, 2013, designating the United States of America and published as International Patent Publication WO 2014/146712 on Sep. 25, 2014, which are hereby incorporated by reference.The present invention is in the field of paper production, more in particular it relates to the process of wood pulping. It provides useful methods and compounds for reducing the energy requirements of the production of wood pulp.Lignin is a major component of wood (seen as brown material), also present in non-wood plants. This heterogeneous polyphenolic compound provides rigidity to the wood structure and protects cellulose fibers from damage. Naturally, lignin creates a major hurdle to ...

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Номер записи: 40

01-01-2015 дата публикации

METHOD FOR SACCHARIFICATION OF BIOMASS

Номер: US20150005484A1

Автор: Kubo Takafumi

Принадлежит:

A method for saccharification of lignocellulosic biomass, the method comprising (1) a pretreatment step of impregnating lignocellulosic biomass with an aqueous alkali solution, subjecting the resultant mixture to solid-liquid separation to remove part of the aqueous alkali solution, and then performing heat treatment, and (2) a saccharification step of enzymatically degrading the lignocellulosic biomass resulting from the pretreatment step to obtain a saccharified liquid can be applied to high-lignin lignocellulosic biomass, reduce the usage of alkali and water in the pretreatment step, increase the sugar yield in the saccharification step, decrease the reaction time, reduce enzyme adsorption on a biomass residue, and improve the enzyme recovery rate. 1. A method for saccharification of lignocellulosic biomass , the method comprising (1) a pretreatment step of impregnating lignocellulosic biomass with an aqueous alkali solution , subjecting the resultant mixture to solid-liquid separation to remove part of the aqueous alkali solution , and then performing heat treatment , and (2) a saccharification step of enzymatically degrading the lignocellulosic biomass resulting from the pretreatment step to obtain a saccharified liquid.2. The saccharification method according to claim 1 , wherein the liquid-solid ratio of the mixture as calculated by Formula (I) is 2 to 20 before solid-liquid separation and 1 to 6 after solid-liquid separation in the pretreatment step.{'br': None, 'Liquid-solid ratio=(total mass of all liquid components in mixture)/(mass of solid matter of lignocellulosic biomass in mixture)\u2003\u2003Formula (I)'}3. The saccharification method according to claim 1 , wherein the heat treatment in the pretreatment step is performed at 100 to 200° C.4. The saccharification method according to claim 1 , wherein the saccharification step is performed in the presence of solubilized lignocellulosic biomass that is a pretreatment-degradation product resulting from ...

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Номер записи: 41

20-01-2022 дата публикации

Two-Times-Two Tank Process and System

Номер: US20220017397A1

Автор: Andreas Helland Lillebø, Hans Rasmus Holte

Принадлежит: Cambi Technology AS

A method and plant for hydrolysis of a liquid substrate, having a dry solids content of between 2 and 30%, to be used in an anaerobic fermentation, digestion or another process aimed at producing or extracting methane or other valuable substances, which method and plant relies on the use of vacuum and several pulpers and one or several flashtanks in series for step-wise pre-heating and pressure reduction, respectively.

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Номер записи: 42

12-01-2017 дата публикации

BIOMASS TRANSFORMATION

Номер: US20170009257A1

Автор: COZE FABIEN, DREUXZIGHA ASSIA, LAZZARELLI CLARINE, Leonetti Jean-Paul, WARD NAGIB

Принадлежит:

The present invention provides improved methods of transforming biomass using bacteria. More particularly, the invention discloses improved methods that combine suitable biomass treatment and processing conditions, allowing transformation of biomass and generation of valuable products under industrially-effective conditions 123-. (canceled)24. A method of transforming a biomass , comprisinga) subjecting the biomass to a treatment to increase the hydrolytic power thereof;b) simultaneously with or subsequently to step a), exposing the biomass to an enzymatic activity that degrades polysaccharides in the biomass; and{'i': 'Deinococcus', 'c) simultaneously with or subsequently to step b), exposing the biomass to a bacterium or an extract thereof.'}25. The method of claim 24 , wherein step a) claim 24 , b) and c) are conducted simultaneously.26. The method of claim 24 , wherein step b) and c) are performed simultaneously claim 24 , after step a).27. The method of claim 24 , wherein step a) comprises subjecting the biomass to steam claim 24 , heat claim 24 , pressure claim 24 , pressurized hot water claim 24 , supercritical water claim 24 , mild acid claim 24 , strong acid claim 24 , mild or strong alkaline treatment claim 24 , or ammonia fiber explosion claim 24 , or a combination thereof.28. The method of claim 27 , wherein step a) comprises an acid treatment at a pH comprised between 3 and 6.29. The method of claim 27 , wherein step a) (further) comprises a transient thermic and/or pressure treatment.30. The method of claim 24 , wherein the biomass is pretreated to remove fine particles.31. The method claim 24 , wherein the treated biomass is washed after treatment step a).32. The method of claim 24 , wherein exposing the biomass to an enzymatic activity comprises adding one or several enzymes to the biomass claim 24 , and/or inducing or allowing expression of one or several enzymes from a microorganism.33Deinococcus. The method of claim 32 , wherein said microorganism ...

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Номер записи: 43

12-01-2017 дата публикации

Enhancement of Lignocellulose Saccharification Via A Low Temperature Ionic Liquid Pre-Treatment Scheme

Номер: US20170009265A1

Автор: Farahani Samira Vasheghani, Schall Constance A.

Принадлежит: The University of Toledo

A method for pretreating lignocellulosic biomass having a lignin component, a hemicellulose component, and a cellulose component, for conversion to sugar is disclosed. Also disclosed is the pretreated lignocellulosic biomass resulting from the method. 1. A method of pretreating lignocellulosic biomass having a lignin component , a hemicellulose component , and a cellulose component , for conversion to sugar , the method comprising:contacting the biomass with an oxidizing agent at a first temperature for a first period of time sufficient to at least partially remove or decompose the lignin component, thereby producing LOX biomass;contacting the LOX biomass with an ionic liquid (IL) at a second temperature for a second period of time, thereby producing a first mixture comprising IL and LOX biomass;contacting the first mixture with a solvent, thereby producing a second mixture comprising LOXIL-treated biomass and solvent, wherein the IL is substantially soluble in the solvent and at least one of the cellulose component or the hemicellulose component is substantially insoluble in the solvent; andseparating the LOXIL-treated biomass from the solvent to produce pretreated lignocellulosic biomass.2. The method of claim 1 , wherein the lignocellulosic biomass comprises poplar claim 1 , corn stover claim 1 , switchgrass claim 1 , or a combination thereof.3. The method of claim 1 , wherein the separating comprises mixing the second mixture so as to precipitate the LOXIL-treated biomass from the IL.4. The method of claim 3 , further comprising washing the precipitated LOXIL-treated biomass with the solvent so as to displace the IL.5. The method of claim 3 , further comprising removing liquid from the precipitated LOXIL-treated biomass through filtration.6. The method of claim 1 , further comprising the step of contacting the second mixture with an acid catalyst to hydrolyze at least one of the hemicellulose component or the cellulose component.7. The method of claim 1 , ...

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Номер записи: 44

08-01-2015 дата публикации

Processing biomass

Номер: US20150010967A1

Автор: Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material.

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Номер записи: 45

08-01-2015 дата публикации

Methods for producing end-products from carbon substrates

Номер: US20150010970A1

Автор: Gopal K. Chotani, Jayarama K. Shetty, Jeff P. Pucci, Karl J. Sanford, Manoj Kumar

Принадлежит: DANISCO US INC

The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate.

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Номер записи: 46

27-01-2022 дата публикации

A METHOD OF PRODUCING BIOMASS DEGRADATION PRODUCTS

Номер: US20220025410A1

Автор: GYLLENBORG Morten Enzo, JEPPESEN Martin Dan

Принадлежит: NGF Nature Energy Biogas A/S

A method of producing biomass degradation products from soft biomass is disclosed, the method comprising the steps of—providing a soft biomass, pretreating the soft biomass in a pretreatment step at a pressure below 2 bar by heating the soft biomass to at a pretreatment temperature between 65 and 100 degrees Celsius to obtain a pretreated biomass, hydrolyzing the pretreated biomass in a first hydrolyzation step to obtain a biomass hydrolysate, and posttreating the biomass hydrolysate in a pressurized posttreatment step by heating the biomass hydrolysate to a posttreatment temperature above 150 degrees Celsius to obtain a posttreated biomass, hydrolyzing the posttreated biomass in a second hydrolyzation step, wherein biogas is obtained from at least the first hydrolyzation step or the second hydrolyzation step. 1. A method of producing biomass degradation products from soft biomass , the method comprising the steps of:providing a soft biomass,pretreating the soft biomass in a pretreatment step at a pressure below 2 bar by heating the soft biomass to at a pretreatment temperature between 65 and 100 degrees Celsius to obtain a pretreated biomass,hydrolyzing the pretreated biomass in a first hydrolyzation step to obtain a biomass hydrolysate, andposttreating the biomass hydrolysate in a pressurized posttreatment step by heating the biomass hydrolysate to a posttreatment temperature above 150 degrees Celsius to obtain a posttreated biomass, 'wherein biogas is obtained from at least the first hydrolyzation step or the second hydrolyzation step.', 'hydrolyzing the posttreated biomass in a second hydrolyzation step,'}2. The method according to claim 1 , wherein the pretreatment step is non-pressurized.3. The method according to claim 1 , wherein the method further comprises separating the biomass hydrolysate in a first separation step into a solid fraction and a liquid fraction.4. The method according to claim 1 , wherein the method further comprises separating the ...

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Номер записи: 47

14-01-2016 дата публикации

BIOMASS TREATMENT SYSTEM, SACCHARIDE SOLUTION PRODUCING PROCESS USING BIOMASS AS RAW MATERIAL, AND ORGANIC RAW MATERIAL PRODUCING PROCESS

Номер: US20160010051A1

Автор: Genta Minoru, Kobayashi Seiji, Terakura Seiichi, Uehara Ryosuke

Принадлежит:

This biomass treatment system includes: a hydrothermal decomposition section for decomposing the cellulose, hemicellulose and lignin contained in a biomass as a raw material under high-temperature and high-pressure conditions in a main section, namely a tank having a gas-liquid interface, and thus removing a lignin component and a hemicellulose component from the biomass; a discharge section for discharging a biomass solid (a component insoluble in hot-water) obtained by the decomposition; a slurrying tank which is connected to the discharge section and in which the discharged biomass solid is slurried in water fed thereinto to form a biomass slurry; and a solid-liquid separation apparatus including both a settling tank which is provided on a discharge line for discharging the biomass slurry and in which the biomass slurry is settled and a scooping-up and conveying means for scooping up the biomass solid deposited at the bottom of the settling tank and separating the solid biomass component from water. 1. A biomass treatment system comprising:a biomass treatment section comprising a treatment column having a gas-liquid interface by which a biomass raw material containing cellulose, hemicellulose, and lignin is decomposed under high-temperature, and high-pressure conditions, and thus a lignin component and a hemicellulose component are removed from the biomass;a biomass solid discharge section for discharging a biomass solid treated by the biomass treatment section;a slurrying tank connected to the biomass solid discharge section, into which water is poured and the discharged biomass solid is slurried; anda solid-liquid separation apparatus including a settling tank for settling the biomass slurry, the settling tank being provided downstream of the slurrying tank, and a scooping-up and conveying means for separating water while scooping up the biomass solid settled on a bottom of the settling tank.2. The biomass treatment system according to claim 1 , further ...

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Номер записи: 48

14-01-2016 дата публикации

CONSORTIUM AND PREPARATION OF MICROORGANISMS FOR CATALYZING CELLULOSE HYDROLYSIS, PREPARATION FOR METHANE FERMENTATION SUPPLEMENTATION, COMBINATION PREPARATION, USE THEREOF AND METHOD USING THE SAME

Номер: US20160010114A1

Автор: Ciezkowska Martyna, Drewniak Lukasz, Poszytek Krzysztof, Sklodowska Aleksandra

Принадлежит:

The invention relates to a consortium of microorganisms capable of hydrolyzing cellulose, preferably lignocellulosic biomass, which may comprise the following mixtures of bacterial strains: sp. KP7, KP20 and sp. KP8 (the mixture deposited in PCM under the no. B/), sp. KP14; sp. KP6 and KP16 (the mixture deposited in PCM under the no. B/), sp. KP4, KP5, KP17 and KP22 (the mixture deposited in PCM under the no. B/), sp. KP10; sp. KP1 and KP19 (the mixture deposited in PCM under the no. B/), sp. KP13; sp. KP9 and KP12 (the mixture deposited in PCM under the no. B/), as well as a preparation for hydrolyzing cellulose which may comprise this consortium, a supplement preparation, a combination preparation, and use and method of using the same. 1BacillusOchrobactrumProvidenciaBacillusBacillusProvidenciaBacillusOchrobactrum; Bacillus. A consortium of microorganisms capable of hydrolyzing cellulose , preferably lignocellulosic biomass , characterized in that it comprises the following mixtures of bacterial strains: sp. KP7 , KP20 and sp. KP8 (the mixture deposited in PCM under the no B/00064) , sp. KP14 , sp. KP6 and KP16 (the mixture deposited in PCM under the no. B/00065) , sp. KP4 , KP5 , KP17 and KP22 (the mixture deposited in PCM under the no. B/00066) , sp. KP10; sp , KP1 and KP19 (the mixture deposited in PCM under the no. B/00067) , sp. KP13sp. KP9 and KP12 (the mixture deposited in PCM under the no. B/00068).2. The consortium of microorganisms according to claim 1 , characterized in that the individual strains in each mixture are mixed in equal proportions.3. The consortium of microorganisms according to claim 2 , characterized in that all the mixtures are mixed in an equal quantitative ratio.4. A preparation for hydrolysis of cellulose claim 1 , increasing the efficiency of biogas production in methane fermentation process claim 1 , revival and/or propagation of methanogenic consortia and/or methanogenic microorganisms themselves claim 1 , characterized in that it ...

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Номер записи: 49

14-01-2016 дата публикации

METHOD FOR PRETREATING BIOMASS, AND METHOD FOR PRODUCING SUGAR CONTAINING GLUCOSE AS MAIN COMPONENT

Номер: US20160010131A1

Автор: Taneda Daisuke

Принадлежит:

A biomass pretreatment method includes a first process performed on the biomass so as to improve contact efficiency between the cellulose and/or hemicellulose and the enzymes that degrade the cellulose and/or hemicellulose; and a second process of removing a substance containing at least an organic acid from the biomass subjected to the first process, the second process including placing the biomass subjected to the first process into a container that has a vent communicating with outside and heating the biomass subjected to the first process at a temperature equal to or lower than a boiling temperature of water by using water while leaving the vent constantly open. 1. A method for pretreating a biomass to be supplied to a method for producing saccharides containing glucose as a main component , the method for producing saccharides including degrading cellulose and/or hemicellulose contained in a biomass with enzymes that degrade cellulose and/or hemicellulose , comprising:a first process performed on the biomass so as to improve contact efficiency between the cellulose and/or hemicellulose and the enzymes that degrade the cellulose and/or hemicellulose; anda second process of removing a substance containing at least an organic acid from the biomass subjected to the first process, the second process including placing the biomass subjected to the first process into a container that has a vent communicating with outside and heating the biomass subjected to the first process at a temperature equal to or lower than a boiling temperature of water by using water while leaving the vent constantly open.2. The method for pretreating a biomass according to claim 1 , wherein the first process is a steam explosion process or a diluted sulfuric acid process.3. The method for pretreating a biomass according to claim 1 , wherein the second process involves heating the biomass by using water at 100° C. or lower.4. The method for pretreating a biomass according to claim 3 , wherein ...

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Номер записи: 50

14-01-2021 дата публикации

PRETREATMENT WITH LIGNOSULFONIC ACID

Номер: US20210010036A1

Автор: Foody Brian, MacDonald Daniel G., Tolan Jeffrey S.

Принадлежит:

A process for converting lignocellulosic biomass to glucose or ethanol includes subjecting the lignocellulosic biomass to a lignosulfonic acid pretreatment, wherein the lignosulfonic acid has a concentration of sulfonate groups in acid form that is greater than 0.02 mol/L and a total amount of sulfur dioxide is greater than 15 wt % based on dry weight of lignocellulosic biomass. 1. A process for converting lignocellulosic biomass to a fermentation product , said process comprising:providing the lignocellulosic biomass;subjecting the lignocellulosic biomass to a lignosulfonic acid pretreatment wherein the concentration of lignosulfonic acid is greater than 0.02 mol/L and wherein a total amount of sulfur dioxide is greater than 15 wt % based on dry weight of lignocellulosic biomass, said lignosulfonic acid pretreatment conducted between 110° C. and 150° C. for at least 30 minutes, thereby providing a pretreated slurry comprising cellulose;subjecting at least the cellulose to an enzymatic hydrolysis to provide glucose;subjecting at least the glucose to a microbial fermentation to produce the fermentation product; and,recovering the fermentation product.2. The process according to claim 1 , comprising separating the pretreated slurry into a liquid stream and a solids stream claim 1 , wherein the solids stream comprises the cellulose and the liquid stream comprises xylose and lignosulfonate.3. The process according to claim 2 , comprising washing solids in the solids stream claim 2 , and wherein subjecting at least the cellulose to an enzymatic hydrolysis comprises mixing the washed solids with cellulase and maintaining the mixture at a temperature and for a time effective to convert at least 60% of the cellulose in the lignocellulosic biomass to glucose.4. The process according to claim 1 , wherein subjecting at least the cellulose to an enzymatic hydrolysis comprises mixing a stream comprising the cellulose with cellulase and maintaining the mixture at a temperature ...

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Номер записи: 51

10-01-2019 дата публикации

ARRAY FOR PROCESSING MATERIALS

Номер: US20190010662A1

Автор: Masterman Thomas Craig, Medoff Marshall, PARADIS Robert

Принадлежит:

Materials (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems equipment, and methods are described that can be used to treat feedstock materials, such as cellulosic and/or lignocellulosic materials, using an array of vaults. 1. A treatment operating unit , comprising:a plurality of enclosure systems, each enclosure system including one or more vaults, andwithin each vault, an irradiation device and a treatment conveyor.2. The operating unit of claim 1 , wherein the enclosure systems are arranged in rows.3. The operating unit of claim 2 , wherein the rows extend in a first direction claim 2 , and wherein each enclosure system comprises two or more vaults extending in a direction generally perpendicular to the first direction.4. The operating unit of claim 3 , wherein the first and second vaults of each enclosure share a common wall.5. The operating unit of claim 4 , wherein each first vault is configured to accept untreated biomass from a storage facility claim 4 , and wherein the biomass material is treated in each vault utilizing the irradiation device and the treatment conveyor.6. The operating unit of claim 5 , wherein the first vault of each enclosure system further encloses equipment configured to transfer treated biomass from the first vault to the second vault of the enclosure system.7. The operating unit of claim 1 , wherein the irradiation device comprises an electron accelerator.8. The operating unit of claim 1 , wherein the treatment conveyor comprises a vibratory conveyor.9. A method for producing treated materials claim 1 , the method comprising;partitioning a material into a plurality of material portions,conveying the material portions into a plurality of first vaults, each first vault accepting one of the material portions,treating the material portions in the vaults,conveying the material portions out of the ...

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Номер записи: 52

03-02-2022 дата публикации

METHOD FOR CARRING OUT THE COMBINED OPERATION OF A BIOETHANOL PRODUCTION UNIT AND A BIOGAS UNIT

Номер: US20220033860A1

Автор: BONK Fabian, LÜDTKE Oliver, SCHLIMBACH Michael

Принадлежит: Verbio Verinigte BioEnergie AG

The present invention concerns a method for carrying out the combined operation of a bioethanol production unit and a biogas unit. The method comprises the following steps: 2. The method as claimed in claim 1 , wherein at least 0.2 t TS per tonne of corn meal is added to the mashing step in 1a) in the form of a mixture of thin stillage and whole stillage.3. The method as claimed in claim 1 , wherein at least 0.12 t TS per tonne of corn meal is recycled to the mashing step in 1a) in the form of whole stillage.4. The method as claimed in claim 1 , wherein at least 0.1 t TS of thin stillage per t of mash is recycled to the mashing step in 1a).5. The method as claimed in claim 1 , wherein the cooking stage in 1b) claim 1 , the mash is heated to a maximum of 70° C.6. The method as claimed in claim 1 , wherein the corn meal in 1a) from a dry milling step has a mass fraction of at least 60% of particles with a particle size of <0.5 mm.7. The method as claimed in claim 1 , wherein the pH of the mash in 1a) is adjusted to less than 4.5.8. The method as claimed in claim 1 , wherein the ammonium nitrogen content in the biogas fermenters of the biogas unit is kept at between 6000-9000 ppm.9. The method as claimed in claim 1 , wherein the mean hydraulic residence time for the biogas unit is at least 30 days.10. The method as claimed in claim 1 , wherein the proportion of outflow from the biogas unit per tonne of corn meal in 1a) is at least 0.2 m.11. The method as claimed in claim 1 , wherein the distillation step in 1b) claim 1 , at least 400 liters of ethanol are separated per tonne of corn meal.12. The method as claimed in claim 1 , wherein the mashing step in 1a) claim 1 , at least 100 g of ammonium nitrogen per tonne of corn meal is recycled via the outflow from the biogas unit.13. The method as claimed in claim 1 , wherein the mashing step in 1a) claim 1 , a maximum of 1000 g of ammonium nitrogen per tonne of corn is recycled via the outflow from the biogas unit.14. The ...

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Номер записи: 53

18-01-2018 дата публикации

Solvent-based processes for producing nanocellulose, and compositions and products produced therefrom

Номер: US20180016355A1

Автор: Kimberly Nelson, Ryan O'Connor, Theodora Retsina, Vesa Pylkkanen

Принадлежит: API Intellectual Property Holdings LLC

The present invention provides a process for producing a nanocellulose material, comprising: fractionating a lignocellulosic biomass feedstock in the presence of a solvent for lignin and water, but no acid catalyst, to generate cellulose-rich solids; and then mechanically treating the cellulose-rich solids to form a nanocellulose material comprising cellulose nanofibrils and/or cellulose nanocrystals. Many organic or inorganic solvents are possible. In some embodiments, the solvent for lignin is an oxygenated organic compound, such as a C 1 -C 18 alcohol, e.g. ethanol, ethylene glycol, propanol, propanediol, glycerol, butanol, or butanediol. The solvent for lignin may be an aromatic alcohol, such as phenol, cresol, or benzyl alcohol. The solvent for lignin may be a ketone, an aldehyde, or an ether, such as methyl ethyl ketone or diethyl ether. The solvent for lignin may be a non-oxygenated alkane, olefin, or aromatic hydrocarbon. In some embodiments, the solvent for lignin is an ionic liquid.

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Номер записи: 54

21-01-2016 дата публикации

Process for producing lower alkyl alcohols from cellulosic biomass using microorganisms

Номер: US20160017378A1

Автор: Kim Seon-Woo, Kohn Richard Allen

Принадлежит:

At least one isolated microorganism, which converts at least 10% by weight, and preferably 50% by weight, of cellulosic biomass to a lower alkyl alcohol by direct digestion, and which produces at least 4% by volume of the lower alkyl alcohol in an aqueous-based digestion medium.

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Номер записи: 55

21-01-2016 дата публикации

METHOD FOR PRODUCING ALCOHOLS AND/OR SOLVENTS FROM LIGNOCELLULOSIC BIOMASS WITH WASHING OF THE SOLID RESIDUE OBTAINED AFTER FERMENTATION

Номер: US20160017380A1

Автор: Aymard Caroline, BOUILLON Pierre-Antoine, Fleurier Stephanie, Louret Sylvain, PEROTTA Larissa, TOTH Eszter

Принадлежит: IFP ENERGIES NOUVELLES

The process for the production of alcohol and/or solvent from a biomass feedstock comprises the stages for pretreatment (P) of the biomass feedstock, for enzymatic hydrolysis (H and HF), and for fermenting the hydrolyzate (HF). To prevent solids from being sent and to facilitate operating the section for purifying the fermentation products, at least a portion of the solid material in the fermentation wine is extracted (Ex) to obtain a stream of solid residue () comprising lignin and a fermentation wine () that is low in solid material. Then, the stream of solid residue is washed (L) with a liquid stream to recover a liquid stream that is enriched with fermentation products (). The liquid stream that is enriched with fermentation products () is recycled in the enzymatic hydrolysis stage (H) to recover any fermentation product and to increase the overall yield of the process.

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Номер записи: 56

21-01-2016 дата публикации

Pretreatment and fractionation of lignocellulosic biomass

Номер: US20160017540A1

Автор: Anantharam Prasad Dadi, Praveen Paripati

Принадлежит: Suganit Systems Inc

Method and apparatus for enhanced production of sugars and lignin via fractionation of lignocellulosic biomass through ionic liquid pretreatment and mild alkaline treatment. The resulting biomass is easily fractionated and amenable to efficient and rapid enzymatic hydrolysis or acid hydrolysis and catalytic conversion to valuable products with high recovery of the enzymes used in the hydrolysis.

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Номер записи: 57

18-01-2018 дата публикации

CONTROLLING THE PRETREATMENT OF A SOFTWOOD STARTING MATERIAL

Номер: US20180016607A1

Автор: HÄGGLUND Karin

Принадлежит:

The present invention relates to a method for pretreating a softwood starting material comprising the following steps: a) pretreating the softwood starting material in a pretreatment process to form a slurry; b) measuring the concentration of glucose and the concentration of mannose in the slurry formed in step a); c) calculating a ratio glucose:mannose from the concentrations measured in step b) d) comparing the ratio calculated in step c) to a reference value and if the ratio calculated in step c) differs from the reference value, controlling at least one process parameter of the pretreatment process in response to the difference. 1. Method for pretreating a softwood starting material comprising the following steps:a) pretreating the softwood starting material in a pretreatment process to form a slurry;b) measuring the concentration of glucose and the concentration of mannose in the slurry formed in step a);c) calculating a ratio glucose:mannose from the concentrations measured in step b); andd) comparing the ratio calculated in step c) to a reference value and if the ratio calculated in step c) differs from the reference value, controlling at least one process parameter of the pretreatment process in response to the difference.2. The method according to further comprising the step e1) hydrolyzing the slurry obtained in step a) such that a sugar solution is obtained.3. The method according to further comprising the step e2) anaerobic digestion of the slurry obtained in step a) or of the sugar solution obtained in step e1) such that biogas is obtained.4. The method according to wherein the hydrolyzing in step e1) is enzymatic hydrolysis.515-. (canceled)16. The method according to wherein:if the ratio glucose:mannose is lower than the reference value at least one process parameter of the pretreatment process is controlled such that the severity of the pretreatment is increased, andif the ratio glucose:mannose is higher than the reference value at least one process ...

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Номер записи: 58

21-01-2021 дата публикации

Feed control in conversion of biomass into hydrocarbon fuels and chemicals

Номер: US20210017548A1

Автор: Arsam Behkish, Giovanni Pilloni, Kelsey M. MCNEELY

Принадлежит: ExxonMobil Research and Engineering Co

The present disclosure relates to processes for producing hydrocarbon fuels from lignocellulosic biomass. A process may include introducing biomass to a pretreatment system, and a first separation system forming a pentose-rich stream and a pentose-lean stream. The pentose-lean stream may be introduced to a hydrolysis system forming a hydrolysate and the hydrolysate introduced to a second separation system forming a hexose-rich stream and a lignin stream. Additionally, at least one of the pentose-rich stream or the hexose-rich stream may be introduced to a bioreactor containing microorganisms configured to produce hydrocarbon fuels. Additionally, the present disclosure also relates to systems for the production of hydrocarbon fuels. A system may include a pretreatment system, a first separation system, a hydrolysis system, a second separation system, and one or more bioreactors. Alternatively a system may include a pretreatment system, a hydrolysis system, a sugar separation system, and one or more bioreactors.

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Номер записи: 59

21-01-2021 дата публикации

BIOLOGICAL GUARD BEDS IN CONVERSION OF BIOMASS INTO HYDROCARBON FUELS AND CHEMICALS

Номер: US20210017549A1

Автор: Behkish Arsam, Paek Changyub, Pilloni Giovanni

Принадлежит:

The present disclosure relates to processes for producing hydrocarbon fuels from lignocellulosic biomass. A process may include introducing biomass to a pretreatment system forming a pretreatment effluent and introducing the pretreatment effluent to a hydrolysis system forming a hydrolysate. The hydrolysate may be introduced to a lignin separation system to form a sugar-rich stream and a lignin-rich stream. The sugar-rich stream may be introduced to a purification system comprising at least one toxin converting microorganism or subcellular material to form a purified sugar-rich stream, and the purified sugar-rich stream and one or more sugar converting microorganisms are introduced to a bioreactor configured to produce hydrocarbon fuels. Additionally, the present disclosure also related to systems for production of hydrocarbon fuels including, a pretreatment system, a hydrolysis system, a lignin separation system, a purification system, and at least one bioreactor. 1. A process for producing hydrocarbon fuels from lignocellulosic biomass , the process comprising:introducing biomass to a pretreatment system forming a pretreatment effluent;introducing the pretreatment effluent to a hydrolysis system forming a hydrolysate;introducing the hydrolysate to a lignin separation system to form a sugar-rich stream and a lignin-rich stream;introducing the sugar-rich stream to a purification system comprising at least one toxin converting microorganism or subcellular material from toxin-converting microorganisms to form a purified sugar-rich stream; andintroducing the purified sugar-rich stream and one or more sugar converting microorganisms to a bioreactor configured to produce hydrocarbon fuels.2. The process of claim 1 , wherein the purification system comprises a subcellular material comprising an enzyme.3. The process of claim 1 , wherein the purification system comprises a biofilm comprising the toxin converting microorganism.4. The process of claim 1 , wherein the ...

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Номер записи: 60

18-01-2018 дата публикации

Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Quinone Compound And Uses Thereof

Номер: US20180019412A1

Автор: Feng Xu, Jason Quinlan, Matthew Sweeney

Принадлежит: Novozymes Inc

The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a quinone compound. The present invention also relates to methods of using the compositions.

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Номер записи: 61

28-01-2016 дата публикации

CELLULOSIC BIOFUEL

Номер: US20160024406A1

Автор: Dieker Kurt A., Gerken Christopher Riley William, Hoefling Ryan Edward, Javers Jeremy Edward, Kohl Scott Dennis, Licklider Jonathon Phillip, Malburg Laercio, Rivers Douglas Bernard, Spooner Jesse, Vander Griend Samuel

Принадлежит:

This disclosure describes processes for using a single cellulosic feedstock or a combination of two or more different cellulosic feedstocks with a starch component to produce a fermented product. The process includes separating the components of the cellulosic feedstocks with fractionation, pretreating a component with wet fractionation with chemicals, hydrolysis and fermentation of the pretreated feedstock(s) to produce cellulosic biofuel. The process may include combining the cellulosic feedstock(s) with other components to a cook and/or a fermentation process, distilling and dehydrating the combined components to produce the bio fuel. The process may also include producing a whole stillage stream from the feedstock(s) and mechanically processing the whole stillage stream to produce a high-value protein animal feed. 1. A method integrating processes to an existing plant , the method comprising:identifying an existing plant that converts grain to ethanol, wherein the existing plant comprises a milling process, a cook process, a fermentation process, a distillation process, a dehydration process, an evaporation process, a solid-liquid separation process, a propagation process;adding a fractionation process to separate bran from other components in the feedstock;adding a pretreatment process by using water and heat to break down cellulose and hemicellulose in the bran;adding a hydrolysis and cellulosic fermentation process to hydrolyze the bran with a cellulase enzyme complex/cocktail and to ferment with an organism to produce cellulosic beer, andcombining the cellulosic beer with starch from the grain in the existing plant into the fermentation process to increase overall yield per feedstock unit in the existing plant.2. The method of claim 1 , further comprising releasing steam from the pretreatment process and sending the steam to be used in the processes described above in the existing plant.3. The method of claim 1 , further comprising redirecting cook water ...

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Номер записи: 62

28-01-2016 дата публикации

METHODS USING PERACIDS FOR CONTROLLING CORN ETHANOL FERMENTATION PROCESS INFECTION AND YIELD LOSS

Номер: US20160024531A1

Автор: Fernholz Peter J., Kummet Jay

Принадлежит:

A process for the use of peracid compositions to eliminate and/or control the growth of undesirable bacteria, including contaminating bacteria, in the fermentation production of alcohol is disclosed. Beneficially, the peracid compositions and methods of use of the same do not interfere or inhibit the growth or replication of yeast and have low or no adverse environmental impact. 1. A method for reducing and/or eliminating microbial populations in a fermentation system comprising:applying a peracid composition to sanitize a fermentation system, wherein the peracid composition comprises a short chain peracid and wherein the fermentation system comprises one or more fermentation vessels, pipes and/or components;providing or obtaining a mash sources in said fermentation system; andreducing and/or eliminating a microbial population of yield loss organisms in said fermentation system.2. The method of claim 1 , wherein the yield loss organisms are lactic acid bacteria and/or acetic acid bacteria.3. The method of claim 1 , wherein the peracid composition comprises peroxyformic acid claim 1 , formic acid claim 1 , hydrogen peroxide and water and does not include acetic acid and/or peracetic acid.4. The method of claim 1 , wherein the peracid composition comprises from about 0.5 wt-% to about 5 wt-% peroxyformic acid claim 1 , from about 1 wt-% to about 10 wt-% formic acid claim 1 , from about 5 wt-% to about 97 wt-% water claim 1 , from about 0 wt-% to about 20 wt-% anionic surfactant claim 1 , from about 0 wt-% to about 10 wt-% oxidizing agent; about 0 wt-% to about 35 wt-% inorganic acid claim 1 , and from about 0 wt-% to about 5 wt-% sequestrant.5. The method of claim 1 , further comprising fermenting a fermentable mash in the presence of at least a portion of said peracid composition claim 1 , and yeast in a vessel to produce ethanol and a solids content claim 1 , wherein said peracid composition controls growth of bacteria in the mash without reducing yeast population; ...

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Номер записи: 63

28-01-2016 дата публикации

ELECTROCHEMICAL DETOXIFICATION METHOD OF WOOD-BASED HYDROLYSATE FOR PRODUCING BIOCHEMICALS OR BIOFUELS, AND DETOXIFIED WOOD-BASED HYDROLYSATE

Номер: US20160024535A1

Автор: HA Jeong Myeong, KIM Yunje, LEE Kyung Min, SUH Dongjin, UM Youngsoon

Принадлежит: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Provided is a method for producing a saccharified solution of wood-based biomass having toxicity reduced or removed, comprising the steps of: preparing a saccharified solution in which wood-based biomass has been pretreated by hydrolysis; and reducing toxicity in the saccharified solution using an electrochemical detoxification method. The method is capable of efficiently removing the toxicity of compounds which are produced during the pretreatment process and inhibit growth of microorganisms and fermentation. In addition, the method is capable of increasing the efficiency of production by minimizing sugar loss and additional costs during the toxicity removal process. 1. A lignocellulosic biomass hydrolysate , which is used for fermentation by microorganisms , the hydrolysate being a hydrolysate derived from a lignocellulosic biomass , comprising a phenolic compound reduced by 50 wt % or more as compared to before electrochemical treatment , and comprising a furanic compound reduced by 90 wt % or more as compared to before electrochemical treatment.2. The lignocellulosic biomass hydrolysate according to claim 1 , wherein the hydrolysate comprises p-coumaric acid reduced by 45 wt % or more as compared to before electrochemical treatment.3. The lignocellulosic biomass hydrolysate according to claim 1 , wherein the hydrolysate comprises p-coumaric acid reduced by 70 wt % or more as compared to before electrochemical treatment.4. The lignocellulosic biomass hydrolysate according to claim 1 , wherein the hydrolysate comprises ferulic acid reduced by 25 wt % or more as compared to before electrochemical treatment claim 1 , comprises vanillin reduced by 45 wt % or more as compared to before electrochemical treatment claim 1 , and comprises syringaldehyde reduced by 70 wt % or more as compared to before electrochemical treatment.5. The lignocellulosic biomass hydrolysate according to claim 4 , wherein the hydrolysate comprises furfural and 5-hydroxymethylfurfural (5-HMF) ...

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Номер записи: 64

28-01-2016 дата публикации

NOVEL EXTREME THERMOPHILIC BACTERIA OF THE GENUS CALDICELLULOSIRUPTOR

Номер: US20160024603A1

Автор: Curvers Simon, Svetlichnyi Vitaly

Принадлежит:

A method for converting lignocellulosic biomass material to a carboxylic acid comprising the step of contacting the lignocellulosic biomass material with a microbial culture for a period of time at an initial temperature and an initial pH, thereby producing an amount of a carboxylic acid; wherein the microbial culture comprises an extremely thermophilic bacteria strain selected from the group consisting of sp. DIB041C, sp. DIB087C, sp. DIB103C, sp. DIB104C, sp. DIB107C, sp. DIB101C, and sp. DIB004C, and wherein the lignocellulosic biomass material is converted in a single step process as part of a consolidated bioprocessing (CBP) system. 1CaldicellulosiruptorCaldicellulosiruptor, Caldicellulosiruptor, Caldicellulosiruptor, Caldicellulosiruptor, Caldicellulosiruptor, CaldicellulosiruptorCaldicellulosiruptor. A method for converting lignocellulosic biomass material to a carboxylic acid comprising the step of contacting the lignocellulosic biomass material with a microbial culture for a period of time at an initial temperature and an initial pH , thereby producing an amount of a carboxylic acid; wherein the microbial culture comprises an extremely thermophilic bacteria strain of the genus , wherein the strain is selected from the group consisting of sp. DIB041C , deposited as DSM 25771sp. DIB087C , deposited as DSM 25772sp. DIB103C , deposited as DSM 25773sp. DIB104C , deposited as DSM 25774sp. DIB107C , deposited as DSM 25775sp. DIB101C , deposited as DSM 25178 and sp. DIB004C , deposited as DSM 25177 , and wherein the lignocellulosic biomass material is converted in a single step process as part of a consolidated bioprocessing (CBP) system.2. The method according to claim 1 , wherein the period of time is 10 hours to 300 hours.3. The method according to claim 1 , wherein the period of time is 50 hours to 200 hours claim 1 , 80 hours to 160 hours.4. The method according to claim 1 , wherein the initial temperature is in the range between 55° C. and 80° C. claim 1 , ...

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Номер записи: 65

26-01-2017 дата публикации

BIOMASS LIQUEFACTION PROCESSES, AND USES OF SAME

Номер: US20170022523A1

Автор: Kim Youngmi, Ladisch Michael R., Mosier Nathan

Принадлежит:

Described are processes for the liquefaction of lignocellulosic biomass under the digestive action of dicarboxylic acid(s). Such digests can exhibit enhanced flowability, reduced volume, and significant biomass conversion to dissolved components, and can in some embodiments be further liquefied by contact with an enzyme. Products resultant of these steps can be used for their sugar content to manufacture biofuels or other products. 19-. (canceled)10. A method for liquefaction of wood biomass , comprising:forming a mixture including an aqueous solution of a dicarboxylic acid and solid, particulate wood biomass; andincubating the mixture for a period of time and under conditions effective to cause hydrolysis of the wood biomass substantially by the dicarboxylic acid to form a flowable composition.11. The method of claim 10 , wherein the solid claim 10 , particulate wood biomass comprises elongate fibrous particles having an average length of at least 1 mm.12. The method of claim 10 , wherein the dicarboxylic acid is selected from maleic acid claim 10 , succinnic acid claim 10 , and oxalic acid.13. The method of claim 12 , wherein the dicarboxylic acid is maleic acid.141. The method of claim claim 12 , wherein said adding is conducted so as to achieve an initial loading of the wood biomass of at least 15% w/w.15. The method of claim 10 , wherein said incubating is conducted sufficiently to liquefy at least 10% by weight of the wood biomass dry matter.16. The method of claim 10 , also comprising the step of neutralizing the flowable composition to form a neutralized composition.17. The method of claim 10 , wherein the dicarboxylic acid constitutes greater than 50% claim 10 , on a molar basis claim 10 , of the organic protic species in the aqueous solution.18. The method of claim 10 , wherein the aqueous solution is essentially free of organic protic species other than the dicarboxylic acid.19. A method for liquefaction of lignocellulosic biomass claim 10 , comprising: ...

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Номер записи: 66

26-01-2017 дата публикации

PROCESSING BIOMASS

Номер: US20170022530A1

Автор: Fung Jennifer Mon Yee, LYNCH James J., Masterman Thomas Craig, Medoff Marshall, YOSHIDA Aiichiro

Принадлежит:

Provided are methods of inducing enzymes, and for treating cellulosic and lignocellulosic biomass with the enzyme. 1. A method , the method comprising:treating a lignocellulosic material with a beam of electrons, wherein the electrons have an energy level range of between 0.8 MeV and 1.8 MeV and wherein the treating is performed to a dose of between 2 and 50 Mrad;milling the treated lignocellulosic material to a particle size of between 30 and 1400 μm; and{'i': 'Trichoderma reesei', 'combining the milled material with to induce enzyme production.'}2. The method of claim 1 , wherein the lignocellulosic material has been treated to reduce its recalcitrance by a treatment method selected from the group consisting of: sonication claim 1 , oxidation claim 1 , pyrolysis claim 1 , steam explosion claim 1 , chemical treatment claim 1 , mechanical treatment claim 1 , and freeze grinding.3. The method of claim 1 , wherein the milling comprises dry milling.4. The method of claim 1 , wherein the milling comprises wet milling.5. The method of claim 1 , wherein the treated lignocellulosic material is milled to a particle size of between 30 and 200 μm.6. The method of claim 1 , wherein the treated lignocellulosic material is milled to a particle size of between 30 and 50 μm.7. The method of claim 1 , wherein the lignocellulosic material is selected from the group consisting of wood claim 1 , grasses claim 1 , and agricultural residues.8sorghum,. The method of claim 1 , wherein the lignocellulosic material is selected from the group consisting of: paper claim 1 , paper products claim 1 , paper waste claim 1 , paper pulp claim 1 , pigmented papers claim 1 , loaded papers claim 1 , coated papers claim 1 , filled papers claim 1 , magazines claim 1 , printed matter claim 1 , printer paper claim 1 , polycoated paper claim 1 , card stock claim 1 , cardboard claim 1 , paperboard claim 1 , cotton claim 1 , wood claim 1 , particle board claim 1 , forestry wastes claim 1 , sawdust claim 1 , ...

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Номер записи: 67

28-01-2016 дата публикации

PROCESS FOR THE TREATMENT OF LIGNOCELLULOSIC BIOMASS

Номер: US20160024712A1

Автор: GRISEL Rudie Johan Hendrik, Huijgen Wouter Johannes Joseph, Smit Adrianus Theodorus

Принадлежит: STICHTING ENERGIEONDERZOEK CENTRUM NEDERLAND

Lignocellulosic biomass can be fractionated for the purpose of increasing cellulose purity in the pulp, increasing native lignin content of the isolated lignin, and improving cellulose hydrolysis, by performing the steps of: (a) extracting the biomass with an extracting liquid comprising at least 20 wt % of a first organic solvent at a temperature below 100° C.; (b) treating the extracted biomass with a treatment liquid comprising a second organic solvent selected from lower alcohols, ethers and ketones, optionally water and optionally an acid, at a temperature between 120° C. and 280° C., and, optionally: (c) subjecting a cellulose-enriched product stream resulting from step (b) to enzymatic hydrolysis. The first and second organic solvent may be different or the same; in particular they comprise ethanol or acetone. 117-. (canceled)18. A process for fractionating lignocellulosic biomass , comprising:{'sub': '2', '(a) extracting the biomass with an extracting liquid comprising at least 50 wt % of a first organic solvent selected from lower alcohols and diols, ethers, ketones, amides, lower alkanes, carboxylic acids and CO(sc), at a temperature below 100° C., wherein the amount of extracting liquid is 0.5-20 L per kg biomass;'}(b) subjecting the extracted biomass to an organosolv step with a treatment liquid comprising a second organic solvent selected from lower alcohols, ethers and ketones, water and optionally an acid, at a temperature between 120° C. and 280° C.19. The process according to claim 18 , wherein the first organic solvent is a water-miscible solvent selected from lower alcohols claim 18 , ethers and ketones.20. The process according to claim 19 , wherein the first organic solvent comprises ethanol.21. The process according to claim 18 , wherein the extracting liquid comprises between 0 and 30 vol. % of water.22. The process according to claim 18 , wherein extraction step (a) is performed at a temperature between 30° C. and 80° C.23. The process ...

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Номер записи: 68

25-01-2018 дата публикации

IN-SITU BIOSTIMULATION OF THE HYDROLYSIS OF ORGANIC MATTER FOR OPTIMIZING THE ENERGY RECOVERY THEREFROM

Номер: US20180023106A1

Автор: CACHO RIVERO Jesùs Andrés, FDZ-POLANCO IÑIGUEZ DE LA TORRE Maria, MANSOUR Alicia

Принадлежит:

Some embodiments are directed to a process for the treatment of organic waste which couples in situ biostimulation to produce hydrolytic enzymes and hydrolysis of the refractory organic matter from waste using these enzymes with a view to energy recovery. 118-. (canceled)19. A process for the treatment of a first , at least partially organic and at least partially solid , substrate , comprising:A. introduction of an initial volume of said first substrate to be treated into at least one hydrolysis reactor;B. introduction of an initial volume of second substrate into at least one biostimulation reactor;{'b': '11', 'C. biostimulation of the second substrate contained in said biostimulation reactor, under aerobic conditions, at a temperature of between 20° C. and 40° C., a pH of between 4 and 7, a moisture level of between 50% and 80% and a residence time of between 1 and 5 days, to ensure at least partial hydrolysis of the organic portion () of said substrate and the in situ production of hydrolytic enzymes;'}{'b': 5', '31, 'D. percolation of a liquid through said volume of second substrate contained in said biostimulation reactor, in order to form a first leachate () enriched in hydrolytic enzymes ();'}E. injection of the first leachate enriched in hydrolytic enzymes into at least one hydrolysis reactor containing said first substrate to be treated; andF. hydrolysis of the first substrate at least partially by the first enriched leachate; wherein the succession of the steps C and D define a biostimulation cycle.20. The process as claimed in claim 19 , in which the hydrolytic enzymes are produced by filamentous fungi.21TrichodermaAspergillusPleurotusPenicilliumFomitopsis. The process as claimed in claim 20 , in which the filamentous fungi belong to the group consisting of the fungi sp. claim 20 , sp. claim 20 , sp. claim 20 , sp. claim 20 , and sp.22. The process as claimed in claim 19 , wherein the succession of the steps C and D defining a biostimulation cycle is ...

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Номер записи: 69

25-01-2018 дата публикации

METHODS FOR MITIGATING THE INHIBITORY EFFECTS OF LIGNIN AND SOLUBLE PHENOLICS FOR ENZYMATIC CONVERSION OF CELLULOSE

Номер: US20180023107A1

Автор: Kim Young Mi, Ladisch Michael Ralph

Принадлежит: PURDUE RESEARCH FOUNDATION

Disclosed herein are methods for improving ethanol production from biomass sources by blocking cellulose from binding to lignin. 1. A method for improving enzymatic digestibility of lignocellulose , comprising:pretreating a lignocellulose feedstock comprising cellulose, hemicellulose, and lignin with at least one hydrothermal pretreatment step at a temperature above 140° C. to produce a pretreated lignocellulose feedstock;solubilizing at least 50% of the hemicellulose by weight in the pretreated lignocellulose feedstock to release sugar oligomers including xylo-oligosaccharides from the hemicellulose;washing the pretreated lignocellulose feedstock after the at least one hydrothermal pretreatment step to remove solids comprising the sugar oligomers and phenolic compounds;forming a slurry of washed pretreated lignocellulose feedstock;blocking the lignin retained in the slurry from binding with a cellulase enzyme by adding a non-specific binding protein to the washed pretreated lignocellulose feedstock slurry; andadding a cellulase enzyme to the washed pretreated lignocellulose feedstock slurry.2. The method of claim 1 , wherein the lignocellulose feedstock comprises a component of corn.3. (canceled)4. The method of claim 3 , wherein the washing step further comprises using water of between about 0.5 to about 15 times the weight of the biomass.5. The method of claim 4 , wherein the water is recycled water.6. The method of claim 5 , wherein the washing step further comprises using distillation column bottoms.7. The method of claim 1 , wherein the washing step is conducted at room temperature.8. The method of claim 1 , wherein the washing step is conducted at temperatures up to 140° C. claim 1 , wherein the washing step comprises pressure washing.9. (canceled)10. The method of claim 1 , wherein the washing step is carried out in at least two stages claim 1 , wherein the first washing stage comprises washing with water having a temperature between about 50° C. and about ...

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Номер записи: 70

24-01-2019 дата публикации

Polypeptides Having Cellulolytic Enhancing Activity And Nucleic Acids Encoding Same

Номер: US20190023751A1

Автор: Brown Kimberly, Harris Paul, Lopez de Leon Alfredo, McFarland Keith, Re Edward, Vlasenko Elena, Zaretsky Elizabeth

Принадлежит: NOVOZYMES, INC.

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. 173-. (canceled)74. A method for degrading a cellulosic material , comprising: treating the cellulosic material with a composition comprising one or more cellulolytic proteins and a GH61 polypeptide having cellulolytic enhancing activity , wherein the composition comprising the GH61 polypeptide having cellulolytic enhancing activity increases the degradation of cellulosic material compared to a composition without the GH61 polypeptide having cellulolytic enhancing activity , and wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of:(a) a GH61 polypeptide comprising an amino acid sequence having at least 90% sequence identity to amino acids 19 to 226 of SEQ ID NO: 8;(b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with the full-length complement of nucleotides 55 to 678 of the polynucleotide of SEQ ID NO: 7, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;(c) a GH61 polypeptide encoded by a polynucleotide having at least 90% sequence identity to nucleotides 55 to 678 of the polynucleotide of SEQ ID NO: 7; and(d) a fragment of the GH61 polypeptide of (a), (b), or (c), wherein the fragment has cellulolytic enhancing activity.75. The method of claim 74 , wherein the GH61 polypeptide comprises an amino acid sequence having at least 90% sequence identity to amino acids 19 to 226 of SEQ ID NO: 8.76. The method of claim 74 , wherein the ...

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Номер записи: 71

23-01-2020 дата публикации

PROCESSES FOR PRODUCING FERMENTATION PRODUCTS

Номер: US20200024620A9

Автор: Clark Suzanne, Craig Joyce, Deinhammer Randall, Hjulmand Anne Glud, Kang Zhengfang, Matthews John, Soong Chee-Leong

Принадлежит: NOVOZYMES A/S

The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention. 1. A process for producing fermentation products from starch-containing material comprising the steps of: an alpha-amylase;', 'optionally a protease having a thermostability value of more than 20% determined as Relative Activity at 80° C./70° C.; and', 'optionally a carbohydrate-source generating enzyme;, 'i) liquefying the starch-containing material at a temperature above the initial gelatinization temperature usingii) saccharifying using a carbohydrate-source generating enzyme;iii) fermenting using a fermenting organism;wherein a cellulolytic composition is present or added during fermentation or simultaneous saccharification and fermentation.2. The process of claim 1 , wherein the pH during liquefaction is between above 5.0-6.5 claim 1 , such as above 5.0-6.0 claim 1 , such as above 5.0-5.5 claim 1 , such as between 5.2-6.2 claim 1 , such as around 5.2 claim 1 , such as around 5.4 claim 1 , such as around 5.6 claim 1 , such as around 5.8.3. The process of claim 1 , wherein the fermentation product is an alcohol claim 1 , preferably ethanol claim 1 , especially fuel ethanol claim 1 , potable ethanol and/or industrial ethanol.4Bacillus,Bacillus stearothermophilus,Bacillus stearothermophilus. The process of claim 1 , wherein the alpha-amylase is from the genus such as a strain of in particular a variant of a alpha-amylase claim 1 , such as the one shown in SEQ ID NO: 1 herein.5Bacillus stearothermophilus. The process of claim 4 , wherein the alpha-amylase has a double deletion of positions I181+ ...

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Номер записи: 72

29-01-2015 дата публикации

Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase Activity and Beta-Xylosidase Activity And Polynucleotides Encoding Same

Номер: US20150031082A1

Автор: Morant Marc Dominique

Принадлежит:

The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 1. A nucleic acid construct or recombinant expression vector comprising an isolated polynucleotide encoding a polypeptide , wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host , wherein the polypeptide having beta-glucosidase activity is selected from:(a) a polypeptide having at least 95% sequence identity to amino acids 20 to 776 of SEQ ID NO: 6;(b) a polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with (i) nucleotides 58 to 2630 of SEQ ID NO: 5 or a cDNA sequence thereof, or (ii) the full-length complement of (i), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;(c) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to nucleotides 58 to 2630 of SEQ ID NO: 5 or a cDNA sequence thereof; and(d) an isolated fragment of the polypeptide of (a), (b), or (c) that has beta-glucosidase activity.2. A recombinant host cell comprising the nucleic acid construct or recombinant expression vector of .3. A nucleic acid construct comprising a heterologous nucleic acid operably linked to an isolated polynucleotide encoding a signal peptide comprising amino acids 1 to 19 of SEQ ID NO: 6.4. The nucleic acid construct of claim 3 , wherein the nucleic acid is operably linked to an isolated polynucleotide ...

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Номер записи: 73

02-02-2017 дата публикации

Useful halophilic, thermostable and ionic liquids tolerant cellulases

Номер: US20170029856A1

Автор: Datta Supratim, Rubin Edward M., Simmons Blake A., Zhang Tao

Принадлежит:

The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide. 1. A method of hydrolyzing a cellulose , comprising: (a) providing a composition comprising (i) an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:5 , wherein said isolated or recombinant polypeptide comprises a catalytic domain , a fibronectin domain 3 (FN-3) and an Ig-like domain , and has a halophilic thermostable or thermophilic cellobiohydrolase (CBH) activity , (ii) a suitable salt concentration , (iii) an ionic liquid (IL) , and (iv) a cellulose; and (b) incubating the composition for a suitable length of time , such that the cellulose is hydrolyzed by the polypeptide.2. The method of claim 2 , wherein the composition comprises a pretreatment biomass.3. The method of claim 2 , wherein the amino acid sequence has at least 80% identity with the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:5.4. The method of claim 3 , wherein the amino acid sequence has at least 90% identity with the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:5.5. The method of claim 4 , wherein the amino acid sequence has at least 95% identity with the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:5.6. The method of claim 5 , wherein the amino acid sequence has at least 99% identity with the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:5.7. The polypeptide of claim 6 , wherein the amino acid sequence of the polypeptide comprises the ...

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Номер записи: 74

01-02-2018 дата публикации

PROCESS FOR TREATING LIGNOCELLULOSIC FEEDSTOCK COMPRISING WET OXIDATION

Номер: US20180029896A1

Автор: Dechman John, Foody Brian, Foody Patrick J.

Принадлежит:

A process for producing one or more products from a lignocellulosic feedstock comprising treating the lignocellulosic feedstock to produce sugar in one or more stages comprising addition of acid, base or a combination thereof and introduction of heat. The addition of the acid, base, or a combination thereof produces a salt. The sugar is fermented to produce a fermentation product and the fermentation product is recovered. A stream comprising the salt is treated by wet oxidation with the introduction of heat. A stream comprising salt resulting from the wet oxidation is provided for use as a salt product or a process chemical for introduction within the process. Heat from the wet oxidation may be supplied to any stage of the process in which heat is introduced. 1. A process for producing one or more products from a lignocellulosic feedstock comprising: 'wherein step (i) optionally comprises addition of cellulase enzymes to produce glucose;', '(i) treating the lignocellulosic feedstock to produce sugar in one or more stages comprising addition of acid, base, or a combination thereof, wherein the addition of acid, base or a combination thereof provides at least one of a sulfite, a sulfite salt, sulfurous acid, and a sulfonic acid,'}(ii) fermenting sugar produced in one or more of the stages of step (i) to produce a fermentation product;(iii) recovering the fermentation product, thereby producing a stream of concentrated fermentation product; (a) lignin,', '(b) the at least one of a sulfite, a sulfite salt, sulfurous acid, and a sulfonic acid, or', '(c) a combination thereof; and, '(iv) treating a process stream by wet oxidation to produce a treated stream comprising a salt, the process stream originating from the process and comprising(v) providing a stream comprising salt from the treated stream of step (iv) for use as a salt product, as a process chemical for introduction within the process, or a combination thereof.2. A process for producing one or more products from ...

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Номер записи: 75

04-02-2016 дата публикации

PROCESSING BIOMASS AND PETROLEUM CONTAINING MATERIALS

Номер: US20160032341A1

Автор: Medoff Marshall

Принадлежит:

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation. 1. A method comprising:processing a cellulosic or lignocellulosic material that has been exposed to an ion beam generated by an accelerator, to produce a product or intermediate;the ion beam providing a radiation dose of greater than 10 Mrad to the cellulosic or lignocellulosic material.2. The method of claim 1 , where in the accelerator is selected from the group consisting of: a pulsed accelerator claim 1 , a continuous wave accelerator claim 1 , a LINAC accelerator claim 1 , and a DC accelerator.3. The method of claim 2 , wherein the DC accelerator device comprises a DC insulated core transformer (ICT) type system.4. The method of claim 1 , wherein the radiation dose is provided at a dose rate of about 1 Mrad/s to about 10 Mrad/s.5. The method of claim 1 , wherein the exposure provides a dose rate of at least 1 Mrad/s.6. The method of claim 1 , wherein the cellulosic or lignocellulosic material is saccharified utilizing an enzyme.7. The method of claim 1 , wherein the accelerator has an output power of at least 50 kW.8. The method of claim 1 , wherein the radiation dose is between about 10 and 50 Mrad.9. The method of claim 1 , wherein the accelerator has an output power of at least 75 kW.10. The method of claim 1 , wherein the accelerator has an output power of at least 100 kW.11. The method of claim 1 , wherein the radiation dose is between 10 and 100 Mrad.12. The method of claim 1 , wherein the radiation dose is between 10 and 50 Mrad.13. The method of claim 1 , further comprising converting the saccharified cellulosic or lignocellulosic material into a product.14. The method of claim 13 , wherein the product is an alcohol.15. The method of claim 1 ...

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Номер записи: 76

01-02-2018 дата публикации

PROCESSING BIOMASS

Номер: US20180030655A1

Автор: Masterman Thomas Craig, Medoff Marshall

Принадлежит:

Methods of manufacturing fuels are provided. These methods use often difficult to process lignocellulosic materials, for example crop residues and grasses. The methods can be readily practiced on a commercial scale in an economically viable manner, in some cases using as feedstocks materials that would otherwise be discarded as waste. 1. A method comprising:irradiating a lignocellulosic material at a dose rate of at least 0.5 Mrad/sec, with an electron beam operating at a voltage of less than 3 MeV and a power of at least 25 kW,combining and agitating the irradiated lignocellulosic material, a liquid medium and a saccharifying agent in a tank by jet mixing to disperse and saccharify the irradiated lignocellulosic material.2. The method of wherein the liquid medium comprises water.3. The method of wherein the saccharifying agent is an enzyme.4. The method of claim 1 , further comprising claim 1 , fermenting the contents of the tank claim 1 , without removing the contents from the tank claim 1 , to produce an alcohol.5. The method of claim 1 , further comprising claim 1 , isolating sugars from the contents of the tank.6. The method of further comprising hammermilling the lignocellulosic material prior to irradiating.7. The method of wherein the lignocellulosic material comprises corncobs.8. The method of wherein irradiating comprises delivering to the lignocellulosic material a total dose of from about 25 to 35 Mrads.9. The method of wherein irradiating comprises multiple passes of irradiation claim 1 , each pass delivering a dose of 20 Mrads or less.10. The method of further comprising soaking the irradiated lignocellulosic material in water at a temperature of at least 40° C. prior to combining the irradiated lignocellulosic material with a liquid medium and a saccharifying agent in a tank. This application is a continuation application of U.S. application Ser. No. 14/794,716, filed Jul. 8, 2015, which is a continuation application of U.S. application Ser. No. 13/ ...

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Номер записи: 77

17-02-2022 дата публикации

Efficient methods and compositions for recovery of products from organic acid pretreatment of plant materials

Номер: US20220049421A1

Автор: Feng Ling

Принадлежит: Pierson Capital Environmental Beijing Ltd

The invention is directed to compositions and processes concerning efficient downstream processing of products derived from organic acids pretreatment of plant materials.

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Номер записи: 78

31-01-2019 дата публикации

METHOD FOR PREPARING SUGAR, BIOETHANOL OR MICROBIAL METABOLITE FROM LIGNOCELLULOSIC BIOMAS

Номер: US20190032094A1

Автор: Eom In Yong, Hong Kyung Sik, JEGAL Jong Geon, Jung Chan Duck, Kim In Chul, LEE Seung Hwan, SONG Bong Keun, Yu Ju Hyun

Принадлежит:

The present invention relates to a method for preparing bioethanol from lignocellulosic biomass. The method of the present invention is capable of: minimizing the impurity content of an enzymatic saccharification raw material, by extracting biomass using hot water, before pretreatment, and removing extractable substances such as inorganic salts; suppressing, to the greatest extent, the production of overdecomposition products of sugar, by pretreating the biomass, from which the hot water extractable substances have been removed, in a condition for maximizing xylan yield; preparing fermentable sugar at a low cost, without washing a pretreated solid obtained from subsequent solid-liquid separation, but by only concentrating a sugar solution obtained after enzymatic saccharification, using a separation film; and preparing bioethanol therefrom in high yield. 133.-. (canceled)34. A method for preparing a sugar solution , the method comprising the steps of:modifying a polyamide nanofiltration membrane with sodium hypochlorite and polyethylene glycol methacrylate (step 1); andfiltering an aqueous sugar solution obtained by hydrolyzing cellulosic biomass using the modified polyamide nanofiltration membrane to recover a refined sugar solution from the non-permeate side and remove fermentation inhibitors from the permeate side (step 2).35. The method according to claim 34 , between the step 1) and the step 2) claim 34 , further comprising:filtering the aqueous sugar solution obtained by hydrolyzing cellulosic biomass using a microfiltration membrane or ultrafiltration membrane to recover a sugar solution from the permeate side (step 1-1).36. The method according to claim 34 , after the step 2) claim 34 , further comprising:filtering the refined sugar solution using a reverse osmosis membrane to recover a refined sugar solution from the non-permeate side and remove fermentation inhibitors from the permeate side (step 2-1).37. The method according to claim 34 , wherein the step ...

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Номер записи: 79

05-02-2015 дата публикации

THERMOPHILIC MUTANTS OF TRICHODERMA REESEI ENDOGLUCANASE I

Номер: US20150037845A1

Автор: Blanch Harvey W., Chokhawala Harshal Akshay, Clark Douglas S., KIM Tae-wan

Принадлежит: The Regents of the University of California

The present disclosure relates to mutant thermostable glycosyl hydrolases family 7 enzymes, including mutant endoglucanase I. In particular, the present disclosure relates to mutant thermostable enzymes, compositions containing the enzymes, and methods of use thereof. 152-. (canceled)53. A mutant thermostable glycosyl hydrolase family 7 enzyme comprising at least two mutations selected from the group consisting of:a) a serine, threonine, leucine, methionine, lysine, alanine, glutamine, glutamic acid, or arginine at an amino acid which aligns with amino acid 230 of SEQ ID NO: 1;b) a leucine or a serine at an amino acid which aligns with amino acid 113 of SEQ ID NO: 1; andc) a threonine or a glycine at an amino acid which aligns with amino acid 115 of SEQ ID NO: 1.54. A mutant thermostable glycosyl hydrolase family 7 enzyme comprising:a) a serine, threonine, leucine, methionine, lysine, alanine, glutamine, glutamic acid, or arginine at an amino acid which aligns with amino acid 230 of SEQ ID NO: 1;b) a leucine or a serine at an amino acid which aligns with amino acid 113 of SEQ ID NO: 1; andc) a threonine or a glycine at an amino acid which aligns with amino acid 115 of SEQ ID NO: 1.55. The mutant thermostable enzyme of claim 53 , wherein the enzyme exhibits increased thermostability as compared to the wild-type enzyme after incubation at about 50° C. for about one hour.56. The mutant thermostable enzyme of claim 53 , wherein the enzyme has a specific activity of at least about 0.5 mMole GE/μMole enzyme/hr after incubation at about 50° C. for about one hour.57. The mutant thermostable enzyme of claim 53 , wherein the enzyme is an endoglucanase or an exoglucanase.58. The mutant thermostable enzyme of claim 53 , wherein the enzyme is derived from fungi or bacteria.59. A composition comprising the mutant thermostable enzyme of .60. An isolated nucleic acid encoding the mutant thermostable enzyme of .61. An expression vector comprising the nucleic acid of operably linked ...

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Номер записи: 80

05-02-2015 дата публикации

METHOD FOR BIOMASS HYDROLYSIS

Номер: US20150037846A1

Автор: Colakyan Manuk, Kayihan Ferhan

Принадлежит: RENMATIX, INC.

Methods and systems for biomass hydrolysis are disclosed. The methods use wash liquor in a sequencing process to maximize sugar yields, particularly Csaccharides. 1. A method comprising , in a processing cycle:forming a mixture comprising fresh water and a first fresh biomass;heating the mixture at a first temperature and a first pressure for a first time period, thereby forming a first liquid fraction and a first solid fraction; 'wherein the first liquid fraction comprises a product composition;', 'redirecting at least a portion of the first liquid fraction from the first solid fraction,'} optionally, wherein the first wash liquor comprises a wash liquor from a previous processing cycle;', 'wherein the washing comprises heating the first solid fraction and the first wash liquor at a second temperature and a second pressure for a second time period, thereby forming a second wash liquor and a second solid fraction;, 'washing the first solid fraction with a first wash liquor;'}removing at least a portion of the second wash liquor from the second solid fraction; andrepeating the processing cycle using a second fresh biomass in place of the first fresh biomass.2. The method of claim 1 ,wherein the portion of the second wash liquor is used as at least a portion of the first wash liquor in the repeating.3. The method of claim 1 ,wherein the first wash liquor comprises a wash liquor from a previous processing cycle.4. The method of claim 1 ,wherein liquid comprising biomass hydrolysate is combined with fresh water to form the first wash liquor.5. The method of claim 1 , further comprising:pressing the second solid fraction to obtain a third solid fraction and a third liquid fraction,wherein at least a portion of the third liquid fraction is used as at least a portion of the first wash liquor in the repeating.6. The method of claim 1 , further comprising:subjecting the second solid fraction to further treatment selected from the group consisting of hydrothermal treatment, ...

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Номер записи: 81

09-02-2017 дата публикации

Processing materials

Номер: US20170036186A1

Автор: Marshall Medoff, Robert Paradis, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, novel systems, methods and equipment for conveying and/or cooling treated biomass are described.

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Номер записи: 82

08-02-2018 дата публикации

Process for Preparing 5-(Chloromethyl)furfural

Номер: US20180037562A1

Автор: Chou Chien-Hung, HUANG Tzu-Chiao, Liao Ruey-Fen, LIU Fang-yu, Lu Xin-An

Принадлежит:

Disclosed herein is a process for preparing 5-(chloromethyl)furfural which includes the steps of: subjecting a cellulosic biomass to a pretreatment so as to obtain a pretreated cellulosic biomass, the pretreatment including a dilute acid treatment and a steam explosion treatment conducted after the dilute acid treatment; mixing the pretreated cellulosic biomass with hydrochloric acid so as to obtain a mixture; reacting the pretreated cellulosic biomass with the hydrochloric acid in the mixture so as to produce a reaction product containing 5-(chloromethyl) furfural; and extracting 5-(chloromethyl)furfural from the reaction product with an organic solvent. 1. A process for preparing 5-(chloromethyl)furfural , comprising the steps of:subjecting a cellulosic biomass to a pretreatment so as to obtain a pretreated cellulosic biomass, the pretreatment including a dilute acid treatment and a steam explosion treatment conducted after the dilute acid treatment;mixing the pretreated cellulosic biomass with hydrochloric acid so as to obtain a mixture;reacting the pretreated cellulosic biomass with the hydrochloric acid in the mixture so as to produce a reaction product containing 5-(chloromethyl)furfural; andextracting 5-(chloromethyl)furfural from the reaction product with an organic solvent.2. The method of claim 1 , wherein the pretreatment further includes an enzymatic hydrolysis treatment using cellulase which is conducted after the steam explosion treatment.3. The method of claim 1 , wherein the pretreatment further includes a solid-liquid separation treatment conducted between the dilute acid treatment and the steam explosion treatment claim 1 , so as to remove a liquid portion of the cellulosic biomass4. The method of claim 1 , wherein the mixing step is conducted under stirring at a temperature ranging from 10° C. to 35° C. for 40 minutes to 150 minutes.5. The method of claim 1 , wherein the pretreated cellulosic biomass in the mixture contains glucanin an amount from ...

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Номер записи: 83

09-02-2017 дата публикации

HYDROTHERMAL-MECHANICAL CONVERSION OF LIGNOCELLULOSIC BIOMASS TO ETHANOL OR OTHER FERMENTATION PRODUCTS

Номер: US20170037436A1

Автор: MONCLIN Jean-Pierre, PYLKKANEN Vesa, RETSINA Theodora, RUTHERFORD Steven R.

Принадлежит:

A low-cost process is provided to render lignocellulosic biomass accessible to cellulase enzymes, to produce fermentable sugars. Some variations provide a process to produce ethanol from lignocellulosic biomass (such as sugarcane bagasse or corn stover), comprising introducing a lignocellulosic biomass feedstock to a single-stage digestor; exposing the feedstock to a reaction solution comprising steam or liquid hot water within the digestor, to solubilize the hemicellulose in a liquid phase and to provide a cellulose-rich solid phase; refining the cellulose-rich solid phase, together with the liquid phase, in a mechanical refiner, thereby providing a mixture of refined cellulose-rich solids and the liquid phase; enzymatically hydrolyzing the mixture in a hydrolysis reactor with cellulase enzymes, to generate fermentable sugars; and fermenting the fermentable sugars to produce ethanol. Many alternative process configurations are described. The disclosed processes may be employed for other fermentation products. 1. A process to produce ethanol from lignocellulosic biomass , said process comprising:(a) introducing a lignocellulosic biomass feedstock to a single-stage digestor, wherein said feedstock contains cellulose, hemicellulose, and lignin;(b) exposing said feedstock to a reaction solution comprising steam or liquid hot water within said single-stage digestor, to solubilize at least a portion of said hemicellulose in a liquid phase and to provide a cellulose-rich solid phase;(c) enzymatically hydrolyzing said cellulose-rich solid phase in a first hydrolysis reactor configured for cellulose liquefaction, thereby providing a first hydrolyzed cellulose mixture containing said liquid phase;(d) refining said first hydrolyzed cellulose mixture in a mechanical refiner to reduce average particle size of said first hydrolyzed cellulose mixture, thereby providing a second hydrolyzed cellulose mixture;(e) enzymatically hydrolyzing said second hydrolyzed cellulose mixture in ...

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Номер записи: 84

07-02-2019 дата публикации

Processing materials

Номер: US20190039023A1

Автор: Marshall Medoff, Robert Paradis, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Materials, such as biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. Conveying systems, such as flowing gas conveying systems and such as closed-loop flowing gas conveying systems are described.

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Номер записи: 85

24-02-2022 дата публикации

METHOD FOR CLEANING A REACTOR FOR PROCESSING A LIGNOCELLULOSIC BIOMASS

Номер: US20220056395A1

Автор: Aymard Caroline, BOURAS GALINIE Meriem, CARNNOT Olivier

Принадлежит:

The present invention concerns a method for cleaning a reactor () for treating a lignocellulosic biomass (P), said method comprising the following steps: 14. A method for cleaning a reactor () for treating a lignocellulosic biomass (P) , said method comprising:a) draining the reactor of the reaction mixture containing the biomass,b) filling the reactor with a basic aqueous solution (EB),c) draining the reactor,d) injecting steam (V) into the reactor,e) filling the reactor with an aqueous solution (E),f) draining the reactor.24. The method as claimed in claim 1 , characterized in that it comprises at least one step a′) of filling the reactor () with an aqueous solution (E) claim 1 , followed by a step a″) of draining the reactor claim 1 , between step a) of draining the reaction mixture and step b) of filling with a basic aqueous solution (EB).34. The method as claimed in claim 1 , characterized in that it further comprises claim 1 , after step a) claim 1 , introducing particles of solid abrasive material into the reactor () claim 1 , either separately or by introducing the particles into the basic solution (EB) or into the or one of the aqueous solution(s) (E).44. The method as claimed in claim 1 , characterized in that the basic aqueous solution (EB) and/or the or one of the aqueous solution(s) (E) is or are heated before introduction into the reactor () claim 1 , in particular to a temperature of at least 20° C. claim 1 , in particular of at least 60° C. claim 1 , and preferably at most 90° C.54. The method as claimed in claim 1 , characterized in that at least step b) of filling the reactor () with the basic aqueous solution (EB) takes place with the interior volume of the reactor at a temperature of at least 60° C. claim 1 , in particular of at least 80° C. claim 1 , in particular of at least 100° C.6. The method as claimed in claim 1 , characterized in that claim 1 , during draining step c) claim 1 , the basic solution drained is recovered for recycling claim 1 ...

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Номер записи: 86

06-02-2020 дата публикации

ENCLOSURES FOR TREATING MATERIALS

Номер: US20200038811A1

Автор: Masterman Thomas Craig, Medoff Marshall, PARADIS Robert

Принадлежит:

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems and methods are described that can be used to treat feedstock materials, such as cellulosic and/or lignocellulosic materials, in two or more vaults that can share a common wall. 115-. (canceled)16. A biomass treatment facility , comprising:a first conveying system configured to convey a biomass material through a first enclosure while exposing the biomass material to a first dose of ionizing radiation from a first electron accelerator to produce a first treated biomass material; anda second conveying system configured to convey the first treated biomass material through a second enclosure while exposing the first treated biomass material to a second dose of ionizing radiation from a second electron accelerator to produce a second treated biomass material.17. The facility of claim 16 , wherein the first and second enclosure share a common wall.18. The facility of claim 16 , wherein each accelerator operates at a power of between about 100 kW and about 300 kW.19. The facility of claim 16 , wherein walls of both the first and second enclosures are fabricated from discrete interconnecting blocks configured to provide a photo-tight structure. This application is a continuation of PCT/US14/21604 filed Mar. 7, 2014 which claims priority to the following provisional applications: U.S. Ser. No. 61/774,684, filed Mar. 8, 2013; U.S. Ser. No. 61/774,773, filed Mar. 8, 2013; U.S. Ser. No. 61/774,731, filed Mar. 8, 2013; U.S. Ser. No. 61/774,735, filed Mar. 8, 2013; U.S. Ser. No. 61/774,740, filed Mar. 8, 2013; U.S. Ser. No. 61/774,744, filed Mar. 8, 2013; U.S. Ser. No. 61/774,746, filed Mar. 8, 2013; U.S. Ser. No. 61/774,750, filed Mar. 8, 2013; U.S. Ser. No. 61/774,752, filed Mar. 8, 2013; U.S. Ser. No. 61/774,754, filed Mar. 8, 2013; U.S. Ser. No. 61/774,775, filed Mar. 8, 2013; U.S ...

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Номер записи: 87

12-02-2015 дата публикации

Biomass processing system, saccharide solution production method using biomass feedstock, alcohol production method

Номер: US20150044729A1

Автор: Hideo Suzuki, Minoru Genta, Seiichi Terakura, Yoshio Kuromi, Yoshitaka Kimura

Принадлежит: Mitsubishi Heavy Industries Mechatronics Systems Ltd

A biomass processing system includes: a hydrolysis processing unit that decomposes, under a high-temperature/high-pressure condition, biomass feedstock in a processing tank having a gas-liquid interface, and removes a lignin component and a hemicellulose component; a biomass solid content discharge unit that discharges a biomass solid content 20 which is a hot water insoluble element; a slurrying vessel that subjects the discharged biomass solid content to slurrying; and a hot water discharge liquid introducing line L 2 that introduces, into the slurrying vessel 21 , a hot water discharge liquid 16 including a biomass hot-water soluble element.

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Номер записи: 88

07-02-2019 дата публикации

High pressure zone formation for pretreatment

Номер: US20190040478A1

Автор: SAMJITSINGH SHARON, Tudman Scott

Принадлежит:

Disclosed herein are methods, systems, and compositions for the uniform pretreatment of biomass within seconds with low inhibitor formation. The pretreatment process is used to convert biomass to a fuel, sugars, or other useful chemicals by subjecting the feedstock to a rapid retention time under pressure and temperature and/or chemical reactant. The system includes at least one high pressure, steam impermiable plug and a continuously-operating valve discharge apparatus to discharge pretreated feedstock while maintaining uniform pressure on the pretreatment system. 1. An industrial scale method for pretreating biomass , the method comprising: (i) a barrel defining an inner chamber comprising a feeder zone and a reaction zone; and', '(ii) one or more rotatable screws configured to move the biomass through the extrusion system from the feeder zone through the reaction zone, wherein the one or more rotatable screws comprise section elements configured to form:', '(1) a preconditioning section in the feeder zone that produces a homogenized biomass by reducing particle size of the biomass and evenly distributing water within the biomass, and', '(2) a high pressure zone that compacts the homogenized biomass to form a steam impervious plug separating the feeder zone and the reaction zone; and, '(a) feeding the biomass into an extrusion system comprising(b) treating the biomass at an elevated temperature and pressure within the reaction zone for less than about one minute to produce a pretreated biomass composition comprising a liquid/solids fraction comprising monosaccharides and solid particles comprising cellulose and/or lignin.2. The method of claim 1 , wherein the extrusion system is configured to pretreat the biomass continuously while maintaining the steam impervious plug for at least about 1 hr claim 1 , at least about 2 hrs claim 1 , at least about 4 hrs claim 1 , at least about 5 hrs claim 1 , at least about 6 hrs claim 1 , at least about 8 hrs claim 1 , at least ...

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Номер записи: 89

18-02-2021 дата публикации

Sscf process for second generation ethanol production from lignocellulosic biomass and 2g residual biomass

Номер: US20210047661A1

Автор: Ajay Kumar Sharma, Ajit Singh, Anshu Shankar Mathur, Deepak Tuli, Manas Ranjan Swain, Ravi Prakash GUPTA, Sankara Sri Venkata Ramakumar, Suresh Kumar Puri

Принадлежит: DEPARTMENT OF BIOTECHNOLOGY, Indian Oil Corp Ltd

The present invention relates to a process for production of ethanol from lignocellulosic biomass via modified simultaneous saccharification and co-fermentation (SSCF), wherein the process includes transferring or recirculation of residual biomass from a first batch of SSCF to a second batch of SSCF at the time of enzymatic hydrolysis and then transferring the residual biomass in part or completely from the second batch of SSCF to a third batch of SSCF process to its hydrolysis reaction step. Overall, the average ethanol yield from three conjugative batch of SSCF is 5.06% higher compare to the single batch of SSCF without adding any additional enzyme, and hydrolysis time.

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Номер записи: 90

18-02-2016 дата публикации

Processing biomass

Номер: US20160046965A1

Автор: Harrison Medoff, Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation.

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Номер записи: 91

18-02-2016 дата публикации

METHODS AND SYSTEMS FOR PRODUCING FERMENTATION PRODUCTS FROM CARBOHYDRATE-RICH SUBSTRATES

Номер: US20160046966A1

Автор: HAMRICK Edward Brian

Принадлежит:

A method for producing fermentation products from lignocellulosic biomass is provided. Lignocellulosic biomass is composed of lignocellulosic fibers which are hollow and primarily contain cellulose, hemicellulose and lignin. Lignin is concentrated in the outer fiber wall and glues the fibers into bundles, but the inner fiber wall has a much lower concentration of lignin and has more easily accessible cellulose and hemicellulose. This method uses vacuum infusion to infuse enzymes into the lumen (hollow center) of lignocellulosic fibers to hydrolyze the hemicellulose and cellulose to produce sugars and oligomers, and then uses cycles of vacuum pressure to pump these homogeneous reagents and sugars and oligomers into and out of the lumen. These reagents are homogenized by mixing the reagents with process water using turbulent mixing to produce a homogeneous reagent. The sugars may be fermented, such as with yeast, to a fermentation product, such as ethanol or butanol. 1. A process for producing a fermentation product from a carbohydrate-rich substrate , said process comprising the steps of:(a) providing a carbohydrate-rich substrate and an aqueous solution containing a hydrolysis catalyst and a fermentation organism, and maintaining a gas pressure above said carbohydrate-rich substrate at an evacuation pressure and for an evacuation time to infuse said aqueous solution into said carbohydrate-rich substrate at an infusion temperature, thereby generating a reaction mixture;(b) exposing said reaction mixture to a gas-phase reaction pressure that is higher than said evacuation pressure;(c) maintaining said reaction mixture at a reaction temperature for a reaction time to generate sugar monomers within lumen space of said carbohydrate-rich substrate;(d) exposing said reaction mixture to a gas-phase expulsion pressure that is lower than said reaction pressure, to pump said sugar monomers out of said lumen space and into said aqueous solution;(e) maintaining said reaction ...

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Номер записи: 92

16-02-2017 дата публикации

METHOD FOR PRETREATING LIGNOCELLULOSE BY USING ACID-BASE MIXTURE CATALYST

Номер: US20170044329A1

Автор: Jung Young Hoon, Kim Kyoung Heon

Принадлежит: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

The present invention relates to a method for pretreating lignocellulose by using an acid-base mixture catalyst. The method pretreats lignocellulose, by using a mixture catalyst of an acid and a base, so as not to pass through additional neutralization steps, and carries out pretreatment and simultaneous saccharification and fermentation through an identical single reactor process, thereby having an effect of producing ethanol in an excellent production yield from lignocellulosic biomass while simplifying the total process and reducing equipment costs and total processing costs. 1. A method for pretreating lignocellulosic biomass comprising:reacting an acid-base mixture catalyst having a pH value of 1 to 4 with the lignocellulosic biomass.2. The method for pretreating lignocellulosic biomass according to claim 1 , wherein the acid is one or more selected from the group consisting of sulfuric acid claim 1 , maleic acid claim 1 , hydrochloric acid claim 1 , nitric acid claim 1 , phosphoric acid claim 1 , carbonic acid claim 1 , formic acid claim 1 , acetic acid claim 1 , hydrofluoric acid claim 1 , oxalic acid claim 1 , and citric acid.3. The method for pretreating lignocellulosic biomass according to claim 1 , wherein the base is one or more selected from the group consisting of sodium hydroxide claim 1 , potassium hydroxide claim 1 , calcium hydroxide claim 1 , barium hydroxide claim 1 , ammonium hydroxide claim 1 , calcium carbonate claim 1 , potassium carbonate claim 1 , and ammonia.4. The method for pretreating lignocellulosic biomass according to claim 1 , wherein the acid and the base are mixed at a molar ratio so that the acid-base mixture catalyst has a total concentration of 0.01 to 1 M.5. The method for pretreating lignocellulosic biomass according to claim 1 , wherein the acid-base mixture catalyst is mixed in an amount of 4 to 20% (w/v) by 1 part by weight of the lignocellulosic biomass.6. The method for pretreating lignocellulosic biomass according to ...

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Номер записи: 93

15-02-2018 дата публикации

COOLING AND PROCESSING MATERIALS

Номер: US20180044705A1

Автор: Medoff Marshall

Принадлежит:

Systems and methods for cooling and processing materials are disclosed. 1. A method comprising:grinding or comminuting a biomass material;cooling the ground or comminuted biomass material to 280° K (6.85° C.) or less to reduce the recalcitrance of the material;after cooling, grinding or comminuting the cooled biomass material; andafter the second grinding or comminuting step, processing the material to produce a product that is chemically different from the biomass material.2. The method of wherein processing comprises contacting the material with an enzyme and/or a microorganism.3. The method of wherein the biomass material comprises a lignocellulosic material.4. The method of wherein the material comprises cellulose and contacting the material comprises utilizing an enzyme to saccharify the cellulose.5. The method of further comprising irradiating the biomass material.6. The method of wherein the biomass material has been irradiated prior to cooling.7. The method of wherein cooling is performed in a freeze grinding or freeze milling device.8. The method of wherein contacting the material comprises utilizing a microorganism to produce an alcohol.9. The method of wherein the alcohol comprises ethanol or n-butanol.10. The method of wherein cooling comprises cooling the material to a temperature below the brittle point of the material.11. The method of wherein cooling is performed prior to irradiating.12. The method of further comprising cooling again after irradiating.13. The method of wherein cooling comprises temperature cycling the starting material.14. The method of wherein the biomass material has a bulk density of less than 0.3 g/cm.15. The method of wherein irradiating is performed using an electron beam.16. The method of wherein the biomass material receives a dose of radiation of from about 10 MRad to 100 Mrad.17. The method of wherein the biomass material receives a dose of radiation of from about 30 MRad to 90 MRad.18. The method of wherein the biomass ...

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Номер записи: 94

15-02-2018 дата публикации

Multi-Stage Enzymatic Hydrolysis of Lignocellulosic Biomass

Номер: US20180044707A1

Автор: Chiara Prefumo, Daniele Riva, David Osborn, Eric Abbate, Geoffrey Moxley, Jesper Frickmann, Johan Belfrage, Micol Purrotti, Piero Otonello, Simone Ferrero, Stefano Paravisi

Принадлежит: Beta Renewables Spa, Novozymes AS

The invention relates to processes of multi-stage hydrolysis where different enzyme compositions are added in at least two stages of hydrolysis. In a first stage, a first enzyme composition of xylanase, a beta-xylosidase and an endoglucanase is added, followed by a latter stage in which a second enzyme composition, a cellulolytic enzyme composition, is added. Also provided are processes for obtaining hydrolysis products and fermentation products using processes of the invention.

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Номер записи: 95

19-02-2015 дата публикации

PROCESSING BIOMASS

Номер: US20150050709A1

Автор: FINN Michael W., Masterman Thomas Craig, Medoff Marshall

Принадлежит:

Some products such as solvents, e.g., butanol, are toxic to solvent-producing microorganisms, yet fructose metabolism can facilitate the increase of protective substrates such as triglycerides, which can protect the microorganism from the toxic effect of the product. Methods are provided for producing a product, e.g., a solvent from fructose derived from a cellulosic or lignocellulosic material. Using the methods herein, a fructose solution can be fermented to a solvent more rapidly, and with better yields, than a glucose solution. 1. A method for producing a product , the method comprising:producing fructose by saccharifying a biomass and contacting the saccharifed biomass with an isomerization agent, and converting the fructose to a product with a microorganism and/or an enzyme.2. The method of claim 1 , wherein the biomass comprises a cellulosic or lignocellulosic material.3. The method of claim 1 , wherein the isomerization agent is an isomerase.4. The method of claim 3 , wherein the isomerase is xylose isomerase.5. The method of claim 2 , wherein the cellulosic or lignocellulosic biomass is treated to reduce its recalcitrance to saccharification.6. The method of claim 5 , wherein the treatment method is selected from the group consisting of: bombardment with electrons claim 5 , sonication claim 5 , oxidation claim 5 , pyrolysis claim 5 , steam explosion claim 5 , chemical treatment claim 5 , mechanical treatment claim 5 , freeze grinding and combination thereof.7. The method of claim 6 , wherein the treatment method is bombardment with electrons.8. The method of claim 2 , wherein the cellulosic or lignocellulosic biomass is selected from the group consisting of: paper claim 2 , paper products claim 2 , paper waste claim 2 , paper pulp claim 2 , pigmented papers claim 2 , loaded papers claim 2 , coated papers claim 2 , filled papers claim 2 , magazines claim 2 , printed matter claim 2 , printer paper claim 2 , polycoated paper claim 2 , card stock claim 2 , ...

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Номер записи: 96

13-02-2020 дата публикации

Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars

Номер: US20200048669A1

Автор: Bertus Noordam, Michael Petrus Jozef BERKHOUT

Принадлежит: DSM IP ASSETS BV

The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

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Номер записи: 97

13-02-2020 дата публикации

PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS

Номер: US20200048671A1

Автор: NOORDAM Bertus

Принадлежит:

A method of treating a patient who has melanoma includes administering to said patient a composition containing a population of activated T cells that selectively recognize cells in the patient that aberrantly express a peptide. A pharmaceutical composition contains activated T cells that selectively recognize cells in a patient that aberrantly express a peptide, and a pharmaceutically acceptable carrier, in which the T cells bind to the peptide in a complex with an MHC class I molecule, and the composition is for treating the patient who has melanoma. A method of treating a patient who has melanoma includes administering to said patient a composition comprising a peptide in the form of a pharmaceutically acceptable salt, thereby inducing a T-cell response to the melanoma. 1. A process for preparation of a sugar product from lignocellulosic material , comprising:enzymatic hydrolysis of the lignocellulosic material in one or more containers using an enzyme composition comprising at least two cellulases to obtain a sugar product, wherein the enzymatic hydrolysis comprises at least:i) a first stage wherein the enzymatic hydrolysis is performed at a first temperature, andii) a second stage wherein the enzymatic hydrolysis is performed at a second temperature, wherein the second temperature is lower than the first temperature and wherein oxygen is added to the hydrolysis during at least part of the second stage.2. A process for the preparation of a fermentation product from lignocellulosic material , comprising the following steps:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a) performing a process according to ,'}b) fermentation of the sugar product to produce a fermentation product; andc) optionally, recovery of the fermentation product.3. The process according to claim 1 , wherein the first temperature is from 50-90° C.4. The process according to claim 1 , wherein the second temperature is from 20-49° C.5. The process according to claim 1 , wherein the first ...

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Номер записи: 98

03-03-2016 дата публикации

CONTINUOUS COUNTERCURRENT ENZYMATIC HYDROLYSIS OF PRETREATED BIOMASS AT HIGH SOLIDS CONCENTRATIONS

Номер: US20160060667A1

Автор: BAYUADRI Cosmas, MONCLIN Jean-Pierre, PYLKKANEN Vesa

Принадлежит:

The invention provides a continuous process for enzymatic hydrolysis of pretreated biomass, the process comprising: providing a pretreated lignocellulosic biomass feed material containing cellulose; introducing the pretreated lignocellulosic biomass feed material to a mechanical-treatment unit containing one or more decompression regions configured to release pressure; introducing a liquid solution containing cellulase enzymes to one or more decompression regions in the mechanical-treatment unit, wherein the liquid solution enters void spaces between fibers of the pretreated lignocellulosic biomass feed material, to form enzyme-containing cellulose-rich solids; and retaining the enzyme-containing cellulose-rich solids under effective hydrolysis conditions to hydrolyze at least some of the cellulose to glucose. Various apparatus configurations are disclosed for the mechanical-treatment unit. 1. A continuous process for enzymatic hydrolysis of pretreated biomass , said process comprising:(a) providing a pretreated lignocellulosic biomass feed material containing cellulose;(b) introducing said pretreated lignocellulosic biomass feed material to a mechanical-treatment unit containing one or more decompression regions configured to release pressure;(c) introducing a liquid solution containing cellulase enzymes to said one or more decompression regions in said mechanical-treatment unit, wherein said liquid solution enters void spaces between fibers of said pretreated lignocellulosic biomass feed material, to form enzyme-containing cellulose-rich solids; and(d) retaining said enzyme-containing cellulose-rich solids under effective hydrolysis conditions to hydrolyze at least some of said cellulose to glucose.2. The process of claim 1 , wherein said enzyme-containing cellulose-rich solids have a solids concentration of about 10 wt % of greater.3. The process of claim 2 , wherein said solids concentration is about 20 wt % of greater.4. The process of claim 1 , wherein said ...

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Номер записи: 99

21-02-2019 дата публикации

PROCESSING BIOMASS

Номер: US20190055615A1

Автор: BAE Seul-a, Masterman Thomas Craig, Medoff Marshall, Valdez Randy

Принадлежит:

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce a product such as a biofuel. 1. A wet milling system comprising a tank with one or more jet mixer heads and one or more wet milling heads disposed within the tank.2. The system of wherein the one or more wet milling heads comprise a rotor and a stator.3. The system of wherein the rotor and the stator include nesting rings of teeth.4. The system of wherein the stator comprises two or more concentric rings of teeth.5. The system of wherein the clearance between the rotor and the stator is from about 0.01 inch to 0.25 inch (0.25 mm to 6.4 mm).6. The system of wherein the spacing between teeth in each ring of teeth is from about 0.1 inch to 0.3 inch (2.5 mm to 7.6 mm).7. A wet milling system comprising one or more jet mixer heads and one or more wet milling heads in a tank comprising liquid medium having biomass dispersed therein.8. The system of wherein the biomass is selected from the group consisting of wood claim 7 , particle board claim 7 , sawdust claim 7 , agricultural waste claim 7 , sewage claim 7 , silage claim 7 , grasses claim 7 , rice hulls claim 7 , bagasse claim 7 , cotton claim 7 , jute claim 7 , hemp claim 7 , flax claim 7 , bamboo claim 7 , sisal claim 7 , abaca claim 7 , straw claim 7 , wheat straw claim 7 , corn cobs claim 7 , corn stover claim 7 , switchgrass claim 7 , alfalfa claim 7 , hay claim 7 , coconut hair claim 7 , seaweed claim 7 , algae claim 7 , and mixtures thereof.9. The system of wherein the agricultural waste is selected from the group consisting of: rice hulls claim 8 , bagasse claim 8 , straw claim 8 , corn stover and coconut hair.10. The system of wherein the silage is alfalfa.11. The system of wherein the biomass comprises lignocellulosic material.12. The ...

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Номер записи: 100