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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 2274. Отображено 100.
28-06-2012 дата публикации

Renewable chemicals and fuels from oleaginous yeast

Номер: US20120164701A1
Автор: Anna Coragliotti, Anthony G. Day, Chung-Soon Im, Donald E. Trimbur, Harrison F. Dillon, Scott Franklin
Принадлежит: Solazyme Inc

The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.

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Номер записи: 1
06-09-2012 дата публикации

Method for the Production of Very Long Chain Fatty Acids (VLCFA) by Fermentation with a Recombinant Yarrowia SP

Номер: US20120226059A1
Автор: Brice Bourdenx, Jean-Denis Faure, Jean-Marc Nicaud, Ramdane Haddouche
Принадлежит: Institut National de la Recherche Agronomique INRA

The present invention concerns a method for the production of Very Long Chain Fatty Acids (VLCFA) by fermentation, comprising culturing a recombinant strain of a Yarrowia sp. comprising a heterologous gene coding for a hydroxyacyl-CoA dehydratase, under control of regulatory elements allowing expression of the said heterologous gene in the said Yarrowia sp. The invention also concerns the recombinant Yarrowia sp.

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Номер записи: 2
27-09-2012 дата публикации

Method of Producing Lauric Acid-containing Oil or Fat

Номер: US20120245368A1
Автор: Fumikazu Takahashi, Hiroshi Yoshida, Yasushi Takimura
Принадлежит: Kao Corp

A method for producing an oil or fat containing lauric acid as a constituent fatty acid including: culturing, in a medium, at least one species of algae in the class Cryptophyceae selected from the group consisting of algae belonging to the genus Rhodomonas and algae belonging to the genus Chroomonas selected from among Chroomonas diplococca, Chroomonas mesostigmatica, Chroomonas nordstedtii , and Chroomonas placoidea and recovering, from the culture product, an oil or fat having a lauric acid content of 3 weight % or higher of the fatty acid composition.

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Номер записи: 3
20-12-2012 дата публикации

Stress-induced lipid trigger

Номер: US20120322157A1
Автор: April Brand, Christopher Yohn, Craig A. Behnke, Michael Mendez
Принадлежит: SAPPHIRE ENERGY INC

The present disclosure provides novel proteins that when over expressed in algae result in an increase or change in fatty acid and/or glycerol lipid production and/or accumulation, without a substantial decrease in the growth rate of the alga or the break down of algal components, such as chlorophyll. The present disclosure also describes methods of using the novel proteins to increase or change the production and/or accumulation of fatty acids and/or glycerol lipids in algae. In addition, these proteins are useful tools in obtaining information about the fatty acid and triacyglyceride (TAG) synthetic pathways in algae.

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Номер записи: 4
31-01-2013 дата публикации

Materials and methods for using an acyl-acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis

Номер: US20130029387A1
Автор: Basil J. Nikolau, Fuyuan Jing, Marna Yandeau-Nelson
Принадлежит: Iowa State University Research Foundation ISURF

A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid encoding a mutant acyl-ACP TE or a chimeric C. viscosissima acyl-ACP TE; a host cell or organism comprising the nucleic acid; a mutant acyl-ACP TE or chimeric C. viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE; and a method of altering the level of activity of a plant acyl-ACP TE.

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Номер записи: 5
28-03-2013 дата публикации

PRODUCTION AND SECRETION OF FATTY ACIDS AND FATTY ACID DERIVATIVES

Номер: US20130078686A1
Автор: BROWN Robert C., Coppersmith Jennifer, Holtzapple Erik, Seshadri Rekha, Verruto John
Принадлежит: Synthetic Genomics, Inc.

The invention relates to acyl-CoA-independent methods of producing fatty alcohols in recombinant host cells engineered to express an alcohol-forming acyl-ACP reductase. The recombinant host cells may be photosynthetic microorganisms, such as cyanobacteria. Isolated nucleic acid molecules, vectors, and recombinant host cells expressing an alcohol-forming acyl-ACP reductase, and systems for producing fatty alcohols via an acyl-CoA-independent pathway, are also provided. Also provided are microorganisms engineered for the secretion of fatty acids and fatty acid derivatives, including fatty alcohols, and methods of producing fatty acid derivatives using such engineering microorganisms. 2. The method of claim 1 , wherein the recombinant microorganism comprises at least one non-native gene encoding a component of a secondary multidrug resistance transporter.3. The method of claim 2 , wherein the secondary multidrug resistance transporter is a member of the Major Facilitator Superfamily (MFS) claim 2 , the Small Multidrug Resistance (SMR) family claim 2 , the Resistance-cell Division (RND) family claim 2 , the Multi Antimicrobial Extrusion (MATE) family claim 2 , and the Putative E Transporter (PET) family.4. The method of claim 3 , wherein the secondary multidrug resistance transporter is a member of the Resistance-cell Division (RND) family.5. The method of claim 5 , wherein the secondary multidrug resistance transporter is a component of a Mex or Acr tranporter.6. The method of claim 4 , wherein the component of a secondary multidrug resistance transporter is an inner membrane permease claim 4 , a membrane fusion protein claim 4 , or an outer membrane factor.7. The method of claim 6 , wherein the component of a secondary multidrug resistance transporter is an inner membrane permease.8. The method of claim 1 , wherein the recombinant microorganism comprises at least one non-native gene encoding a component of a Type VI Secretion System.9Acidovorax, Agrobacterium, ...

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Номер записи: 6
11-04-2013 дата публикации

PROCESS FOR THE PRODUCTION OF BIO-OIL FROM MUNICIPAL SOLID WASTE

Номер: US20130090487A1
Автор: Bosetti Aldo, Franzosi Giuliana
Принадлежит: ENI S.P.A.

A process for producing bio-oil from municipal solid waste, the process including: a) liquifying municipal solid waste, to obtain a mixture containing an oily phase containing bio-oil, a solid phase, and a first aqueous phase; b) treating the first aqueous phase from a) with an adsorbing material, to obtain a second aqueous phase; c) fermenting the second aqueous phase from b), to obtain a biomass; d) subjecting the biomass obtained in c) to the liquification a). The bio-oil obtained is advantageously used in the production of biofuels for motor vehicles or for the generation of electric energy or heat. 1. A process for producing bio-oil from municipal solid waste , the process comprising:a) liquifying the municipal solid waste, to obtain a mixture comprising an oily phase comprising bio-oil, a solid phase, and a first aqueous phase;b) treating the first aqueous phase obtained in a) with at least one an adsorbing material, obtaining to obtain a second aqueous phase;c) subjecting fermenting the second aqueous phase obtained in the treatment step b), obtain a biomass;d) subjecting the biomass obtained in c) to the liquifying a).2. The process of claim 1 , wherein municipal solid waste is an organic material from a sorted waste collection claim 1 , an organic material from unsorted municipal solid waste claim 1 , or a mixture thereof; or a mixture comprising the organic material and pruning cuttings claim 1 , agricultural residues claim 1 , or a mixture thereof.3. The process of claim 2 , wherein the municipal solid waste is employed in a mixture comprising:primary and biological sludge produced in a wastewater purification plant;a residue, a scrap, or a mixture thereof from agricultural and/or zootechnic activities;a residue, a scrap, or a mixture thereof from an argo-food industry;a residue, a scrap, or a mixture thereof from agricultural processing, forestation, and/or silviculture; or any mixture thereof.4. The process of claim 1 , further comprising prior to a): ...

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Номер записи: 7
16-05-2013 дата публикации

YEAST STRAINS AND THEIR USES IN THE PRODUCTION OF LIPIDS

Номер: US20130122557A1
Автор: Apt Kirk E., Barclay William R., Behrens Paul Warren
Принадлежит:

The invention is directed to isolated microorganisms, as well as biomasses, cultures, microbial oils, and compositions thereof. The invention also provides methods of producing the microbial oils and methods of using the isolated microorganisms, biomasses, and microbial oils. 1Pseudozyma aphidis, Pseudozyma rugulosa, Sporidiobolus pararoseusRhodotorula ingeniosa. An isolated microorganism of the species selected from the group consisting of , and , wherein the microorganism is capable of producing a microbial oil comprising less than 30% by weight saturated fatty acids.2. The isolated microorganism of claim 1 , wherein the microorganism is capable of producing fatty acids in an amount that is at least 30% by weight of the dry cell weight.3. The isolated microorganism of claim 1 , wherein the microorganism is capable of producing fatty acids in an amount that is at least 40% by weight of the dry cell weight.4. The isolated microorganism of claim 1 , wherein the microorganism is capable of producing fatty acids in an amount that is at least 50% by weight of the dry cell weight.5. The isolated microorganism of claim 1 , wherein the microorganism is capable of producing fatty acids in an amount that is at least 60% by weight of the dry cell weight.6. The isolated microorganism of claim 1 , wherein the microorganism is capable of producing a microbial oil comprising greater than 30% by weight oleic acid.7. The isolated microorganism of claim 1 , wherein the microorganism is capable of producing a microbial oil comprising greater than 40% by weight oleic acid.8. The isolated microorganism of claim 1 , wherein the microorganism is capable of producing a microbial oil comprising greater than 50% by weight oleic acid.9. The isolated microorganism of claim 1 , wherein the microorganism is capable of producing a microbial oil comprising greater than 60% by weight oleic acid.10. The isolated microorganism of claim 1 , wherein the microorganism is capable of producing a ...

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Номер записи: 8
23-05-2013 дата публикации

OMEGA 7 RICH COMPOSITIONS AND METHODS OF ISOLATING OMEGA 7 FATTY ACIDS

Номер: US20130129775A1
Автор: Kale Aniket, KULAGA Tom, LICAMELE Jason D., Shinde Sandip, Tonkovich Anna Lee
Принадлежит: Heliae Development, LLC

Disclosed here are compositions rich in omega-7 fatty acids, including palmitoleic acid, and products rich in omega-7 fatty acids derived from algal biomass. The algae and/or compositions rich in omega-7 fatty acids may be used in products or as ingredients of products. Methods and systems for increasing the production or concentration of omega-7 fatty acids, and isolating omega-7 fatty acids from algal biomass are also disclosed herein. 1. A composition derived from algae comprising , a total fatty acid profile with a fraction comprising at least about 60% of the total fatty acids with the types C16:0 and C16:1.2. The composition of claim 1 , wherein the C16:1 fatty acids comprise at least about 40% of the fraction of the composition comprising C16:0 and C16:1 fatty acids.3. The composition of claim 1 , wherein the composition is used in a cosmetic claim 1 , medicinal claim 1 , pharmaceutical claim 1 , nutritional claim 1 , food claim 1 , feed claim 1 , or beverage product.4. A composition derived from algae comprising claim 1 , a total fatty acid profile with a fraction comprising at least about 80% of the total fatty acids with the types C16:0 claim 1 , C16:1 claim 1 , C18:0 claim 1 , and C18:1.5. The composition of claim 4 , wherein C16:1 fatty acids comprise at least 30% of the fraction of the composition comprising C16:0 claim 4 , C16:1 claim 4 , C18:0 claim 4 , and C18:1 fatty acids.6. The composition of claim 4 , wherein the composition is used in a cosmetic claim 4 , medicinal claim 4 , pharmaceutical claim 4 , nutritional claim 4 , food claim 4 , feed claim 4 , or beverage product.7. An algal composition comprising algal oil of: at least about 30% C16:0; at least about 25% C16:1; at least about 1% C18:0; and at least about 15% C18:1.8. The composition of claim 7 , wherein the composition is used in a cosmetic claim 7 , medicinal claim 7 , pharmaceutical claim 7 , nutritional claim 7 , food claim 7 , feed claim 7 , or beverage product.9. A product ...

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Номер записи: 9
13-06-2013 дата публикации

Optimization of Lipid Synthesis and Accretion

Номер: US20130149754A1
Автор: Jean-Marc Nicaud, Thierry Dulermo
Принадлежит: Institut National de la Recherche Agronomique INRA

The present invention relates to a novel mutant strain of yeast, particularly a strain of Yarrowia lipolytica , which is capable of accumulating a large quantity of lipids. Said strain is deficient in the beta-oxidation of fatty acids and overexpresses the GPD1 gene. Preferably, the mutant strains, which do not express the GUT2 gene and which are deficient for the beta-oxidation of fatty acids, do not express the POX2 to POX5 genes, very preferably the POX2 to 6 genes. The invention also relates to a method for obtaining the strains of yeast according to the invention and to the use of said strains in the production of lipids.

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Номер записи: 10
13-06-2013 дата публикации

Production of High Levels of DHA in Microalgae Using Modified Amounts of Chloride and Potassium

Номер: US20130150604A1
Автор: Apt Kirk, Behrens Paul W., Fichtali Jaouad, HANSEN Jon, III JOSEPH W., LIPPMEIER James Casey, Pfeifer, THOMPSON John M., WYNN James P.
Принадлежит: DSM IP ASSETS B.V.

Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate , using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains. 121.-. (canceled)22. A method of producing docosahexaenoic acid (DHA) , comprising: chloride ion at a concentration of less than or equal to 3 g/L;', 'potassium ion at a concentration of 0.95 g/L to 10 g/L;', 'wherein the culture medium further comprises sodium ion, wherein a ratio of sodium ion to potassium ion is less than or equal to 27:1; and, 'culturing a heterotrophic microalga in a culture medium, wherein the culture medium comprises{'sup': '9', 'recovering DHA-containing lipid from the microalga, wherein the microalga produces at least 0.04 g DHA per 10cells.'}23. The method of claim 22 , wherein the concentration of chloride ion is less than or equal to 2 g/L.24. The method of claim 22 , wherein the concentration of chloride ion is less than or equal to 1 g/L.25. The method of claim 22 , wherein the concentration of chloride ion is less than or equal to 0.3 g/L.26. The method of claim 22 , wherein the concentration of potassium ion is 0.95 g/L to 4 g/L.27. The method of claim 22 , wherein the concentration of potassium ion is 0.95 g/L to 3 g/L.28. The method of claim 22 , wherein the concentration of potassium ion is 0.95 g/L to 2.4 g/L.29. The method of claim 22 , wherein the concentration of potassium ion is 0.95 g/L to 2 g/L.30. The method of claim 22 , wherein the sodium ion is at a concentration of 1 g/L to 8 g/L.31. The method of claim 22 , wherein the sodium on is at a concentration of 1 g/L to ...

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Номер записи: 11
25-07-2013 дата публикации

Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof

Номер: US20130190520A1
Автор: Jon Milton Hansen, Joseph W. Pfeifer, III, Kirk E. Apt, Paul Warren Behrens, Ross Zirkle, Tracey Lynn Stahl
Принадлежит: DSM IP ASSETS BV

The invention is directed to microbial oils containing omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid, eicosapentaenoic acid, and optionally docosapentaenoic acid and dosage forms containing such oils.

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Номер записи: 12
25-07-2013 дата публикации

Enzymatic modification of oil

Номер: US20130190521A1
Автор: James Colin Barrow, Jaroslav A. Kralovec, Weijie Wang
Принадлежит: Ocean Nutrition Canada Ltd

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.

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Номер записи: 13
01-08-2013 дата публикации

CHEMICAL AND BIOLOGICAL AGENTS FOR THE CONTROL OF MOLLUSCS

Номер: US20130196013A1
Автор: Asolkar Ratnakar, Cordova-Kreylos Ana Lucia, Huang Huazhang, Koivunen Marja, Marrone Pamela, Rackl Sarahann
Принадлежит: MARRONE BIO INNOVATIONS, INC.

Compositions and methods for controlling molluscs, members of the Gastropoda and Bivalvia classes which includes but is not limited to lactones, lactams, carbamates, amides, and/or carboxylic acid containing compounds as active ingredients and/or compounds derived from and/or . Also provided are methods and compositions for increasing the efficacy of chemical and biological control for invasive molluscs in open waters, power plants, and drinking water treatment facilities under coldwater conditions. 1. A combination comprising at least one or more substances effective for controlling a member of a Gastropoda and Bivalvia class and an inert material.2PseudomonasPseudomonas. The combination according to claim 1 , wherein said substances are derived from a species or cell suspension or toxin derived from a species.3Pseudomonas protegensPseudomonas protegens.. The combination according to claim 1 , wherein said substances are derived from a or cell suspension derived from4PseudomonasPseudomonas. The combination according to claim 1 , wherein said substances are derived from a strain claim 1 , having the identifying characteristics of ATCC 55799.5Pseudomonas. The combination according to claim 1 , wherein the composition comprises a substance that is a cell suspension comprising cells having the toxin producing characteristics of ATCC 55799.6. The combination of claim 1 , wherein said combination is a composition.7ErwiniaPseudomonas. A method for controlling one or more molluscs in a location where control is desired comprising introducing into said location at least one of (a) a cell suspension or extract derived from sp. Cells; (b) one or more compounds claim 1 , wherein said compounds are lactone claim 1 , lactam claim 1 , carbamate claim 1 , carboxylic acid and/or amide compounds or composition comprising said compounds claim 1 , with the proviso that said compounds are not gamma-octalactone claim 1 , gamma-nonalactone claim 1 , gamma-decanolactone claim 1 , gamma- ...

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Номер записи: 14
01-08-2013 дата публикации

PROCEDURE FOR THE OBTAINMENT OF FATTY ACIDS OF PHARMACOLOGICAL AND NUTRITIONAL INTEREST

Номер: US20130197086A1
Автор: Stroïazzo Mougin Bernard A. J.
Принадлежит:

This invention refers to a procedure for obtaining fatty acids of pharmacological and nutritional interest that comprises the steps of feeding a gas comprising COinto a reactor that contains a culture that comprises at least one species of microalgae capable of photosynthesis, the process of photosynthesis by the species of microalgae from the COsupplied, producing a biomass that contains a general formula (I) compound: 2. Procedure according to claim 1 , in which in the step in which the culture is at least partially removed from the reactor claim 1 , between 5 and 50% of the culture is removed.3. Procedure according to claim 2 , in which in the step in which the culture is at least partially removed from the reactor claim 2 , approximately 10% of the culture is removed.4. Procedure according to claim 1 , in which before step a) a pre-treatment is carried out with the gas or a mixture of gases comprising COwhich consists of at least one of: substantial elimination of SO claim 1 , NOand moisture and changing the gas temperature to a range of 30 to 40° C.5. Procedure according to claim 1 , in which the microalgae is selected from the group consisting of: Clorophyceae claim 1 , Bacilliarioficeas claim 1 , Dinophyceae claim 1 , Cryptophyceae claim 1 , Chrysophyceae claim 1 , Haptophyceae claim 1 , Prasinophyceae claim 1 , Raphidophyceae claim 1 , Eustigmatophyceae or any combination thereof.6Dunaliella salina, Tetraselmis Galvana, Tetraselmis suecica, Iisochrysis galbana, Nannochloropsis GaditanaNannochloris. Procedure according to claim 5 , in which the species of microalgae is selected from the group consisting of o claim 5 , in any combination thereof.7. Procedure according to claim 1 , in which the gas or mixture of gases that is fed into the reactor in step a) comes exogenously claim 1 , from the atmosphere or any industry and claim 1 , endogenously claim 1 , from the gases generated during the procedure itself claim 1 , in any combination thereof.8. Procedure ...

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Номер записи: 15
15-08-2013 дата публикации

MAGNETOTACTIC ALGAE AND METHODS OF USE

Номер: US20130210064A1
Автор: Nath Pulak, Twary Scott N.
Принадлежит:

Disclosed herein are magnetotactic algae, such as algae cells that include magnetic nanoparticles. In some examples the magnetotactic algae express a nucleic acid molecule encoding a bacterial MagA ferrous transporter, a nucleic acid molecule encoding a bacterial Mms6 magnetite binding protein, or both. Also disclosed herein are methods for producing magnetotactic algae and methods of producing biofuel or magnetic nanoparticles utilizing magnetotactic algae. Further disclosed herein are methods of enriching a population of magnetotactic algae cells (for example, increasing the number of magnetotactic algae cells in a population of algae cells). In further embodiments, disclosed herein are methods of selecting a transformed algae cell. 1. A magnetotactic alga cell , comprising an alga cell expressing a nucleic acid encoding a bacterial MagA ferrous transporter , a nucleic acid encoding a bacterial Mms6 magnetite binding protein , or a combination thereof.2Magnetospirillum magneticum.. The magnetotactic alga cell of claim 1 , wherein the nucleic acid encoding the bacterial MagA ferrous transporter or the nucleic acid encoding the bacterial Mms6 magnetite binding protein is from3Chlamydomonas, Nannochloropsis, Tetraselmis, Botryococcus, Chlorella, Dunaliella, Gracilaria, Pleurochrysis,Sargassum.. The magnetotactic alga cell of claim 1 , wherein the algal cell is or4. A method of producing magnetotactic algae claim 1 , comprising:transforming an alga cell with a nucleic acid encoding a bacterial MagA ferrous transporter, a nucleic acid encoding a bacterial Mms6 magnetite binding protein, or a combination thereof; andcultivating the transformed alga cell or progeny thereof that express the bacterial MagA ferrous transporter, the bacterial Mms6 magnetite binding protein, or both, under conditions sufficient to produce algae comprising magnetic nanoparticles, thereby producing magnetotactic algae.5. The method of claim 4 , further comprising magnetically collecting the ...

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Номер записи: 16
15-08-2013 дата публикации

NOVEL STRAINS OF MICROALGAE OF THE GENUS BOTRYOCOCCUS AND METHOD FOR THE CULTURE OF SAID MICROALGAE IN MIXOTROPHIC MODE

Номер: US20130210095A1
Автор: Calleja Pierre
Принадлежит: FERMENTALG

Novel strains of microalgae which belong to the genus and which can grow in a mixotrophic mode, and a cultivation method which includes providing light in the form of flashes for the production of lipids and hydrocarbons, in particular in the form of botryococcenes, which are useful in the production of biofuel. 1Botryococcus. Method for the culture of microalgae of the genus for the production of lipids or hydrocarbons , characterized in that it comprises the following steps:{'i': 'Botryococcus', 'sup': −2', '−1, 'the culture of one or more strains of microalgae of the genus in darkness in the presence of a supply of light that is discontinuous or variable over time, the intensity of which, in micromoles of photons, varies by an amplitude of more than 10 μmol. m. s, at a rate of at least once per hour;'}the maintenance of said culture over several generations in the presence of a carbon-containing substrate in the culture medium;the harvesting of the cells charged with hydrocarbons or lipids.2. Method according to claim 1 , characterized in that the supply of light is discontinuous.3. Method according to claim 1 , characterized in that the supply of light varies by more than 40 claim 1 , preferably claim 1 , more than 50 μmol. m. s.4. Method according to claim 1 , characterized in that it also comprises the recovery of the lipids or hydrocarbons contained in or excreted by the microalgae.5. Method according to claim 4 , characterized in that the hydrocarbons contained in or excreted by the microalgae comprise botryococcenes.6. Method according to claim 1 , characterized in that the culture medium is a minimum medium comprising a carbon-containing substrate.7. Method according to claim 1 , characterized in that the carbon-containing substrate comprises acetate claim 1 , glucose claim 1 , cellulose claim 1 , starch claim 1 , lactose claim 1 , saccharose or glycerol.8. Method according to claim 1 , characterized in that the supply of light is carried out in the form ...

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Номер записи: 17
10-10-2013 дата публикации

Host Cells and Methods for Producing Diacid Compounds

Номер: US20130267012A1
Автор: Eric J. Steen, Jay D. Keasling, Jeffrey A. Dietrich, Jeffrey L. Fortman
Принадлежит: UNIVERSITY OF CALIFORNIA

The present invention provides for a method of producing one or more fatty acid derived dicarboxylic acids in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived dicarboxylic acids. The invention provides for the biosynthesis of dicarboxylic acid ranging in length from C3 to C26. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism.

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Номер записи: 18
17-10-2013 дата публикации

METHODS FOR ALTERING LIPIDS IN ALGAE AND YEAST

Номер: US20130273620A1
Автор: ANDERSON Lisa Adele, DANIELEWICZ Megan, FRANZ Annaliese Kirsten, WONG Diana Meiying
Принадлежит:

The present disclosure relates to methods of increasing lipid levels in an algal or yeast cell population, methods of producing saturated or monounsaturated triacylglycerols in an algal or yeast cell population, and methods of decreasing polyunsaturated triacylglycerol production in an algal or yeast cell population by contacting the cell population with a chemical compound that is capable of increasing lipid levels or altering the lipid composition in the cell population. 1. A method of increasing lipid levels in a cell population , comprising:contacting an algal or yeast cell population with at least one compound selected from Table 1 in an amount sufficient to increase lipid levels in said cell population, wherein said at least one compound does not inhibit fatty acid metabolism.2. The method of claim 1 , wherein the cell population is an algal cell population.3. The method of claim 1 , wherein the cell population is a yeast cell population.4. The method of claim 1 , wherein the at least one compound comprises a lipoxygenase inhibitor selected from the group consisting of curcumin claim 1 , caffeic acid claim 1 , baicalein claim 1 , esculetin claim 1 , and gossypol.5. The method of claim 1 , wherein the at least one compound comprises a phosphatase inhibitor selected from the group consisting of PTP Inhibitor II claim 1 , ethyl 3 claim 1 ,4-dephostatin claim 1 , cantharidin claim 1 , napthyl acid phosphate claim 1 , dephostatin claim 1 , and 3 claim 1 ,4-dephostatin.6. The method of claim 1 , wherein the at least one compound comprises a kinase inhibitor or kinase activator selected from the group consisting of BPDQ claim 1 , genistein claim 1 , butein claim 1 , emodin claim 1 , piceatannol claim 1 , quinazoline claim 1 , and BPIQ-II claim 1 , forskolin claim 1 , SB202190 claim 1 , pd98059 claim 1 , 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3 claim 1 ,4-d]pyrimidine claim 1 , bisindolylmaleimide claim 1 , bohemine claim 1 , kenpaullone claim 1 , BPIQ-II ...

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Номер записи: 19
17-10-2013 дата публикации

Recombinant Microalgae Cells Producing Novel Oils

Номер: US20130273621A1
Автор: Chua Penelope, ESPINA Karen, Franklin Scott, Rudenko George, Somanchi Aravind
Принадлежит: Solazyme, Inc.

Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus. 127-. (canceled)28. A method comprising:heterotrophically cultivating a microalgal cell, the cell comprising a chromosomal knockout of a stearoyl ACP desaturase allele so that the cell produces an oil having an altered fatty acid profile comprising a reduction in unsaturated fatty acids; andisolating the oil comprising the unsaturated fatty acids from the cell.29. The method of claim 28 , wherein the cell comprises a recombination event in the stearoyl-ACP desaturase allele.30Prototheca.. The method of claim 28 , wherein the microalgal cell is of the genus31. The method of claim 28 , wherein the cell is cultivated on glucose claim 28 , fructose or depolymerized cellulosic material as a carbon source.32. The method of claim 29 , wherein the recombination event is a double reciprocal recombination event.33. The method of claim 29 , wherein a selectable marker gene is incorporated into the chromosome so as to knock out the stearoyl-ACP desaturase allele.34. The method of claim 33 , wherein the selectable marker gene encodes a sucrose invertase enzyme.35. The method of claim 34 , wherein the cell is capable of growth on sucrose as a sole carbon source.36Prototheca moriformis.. The method of claim 35 , wherein the microalgal cell is of the species This application is a continuation of U.S. application Ser. No. 13/479,194, filed May 23, 2012, which is a continuation of U.S. application Ser. No. 13/073,757, filed Mar. 28, 2011, now U.S. Pat. No. 8,187,860, which is a ...

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Номер записи: 20
31-10-2013 дата публикации

FATTY ACIDS FROM PHYTOPLANKTON

Номер: US20130287917A1
Автор: Dicosola Gregory, Harvey Todd, Kao Richard, Randhava Ajaib Singh, Randhava Sarabjit Singh, Randhava Surjit Singh
Принадлежит:

This invention relates to a new process for making fatty acids directly from a feedstock consisting of phytoplankton in water without the need to a) thermally separate, dewater and dry the phytoplankton, and b) extract the immobilized lipids. 1. A continuous process for making fatty acids from a feedstock consisting of phytoplankton in water wherein the weight content of the biomass in the slurry can range from less than 1 wt % up to 35 wt %.2. The process of wherein a “dilute phytoplankton soup” generated in a phytoplankton propagation system (tank claim 1 , shallow pond claim 1 , reactor or other cultivation mechanism) claim 1 , that contains a high percentage by weight of water and a low percentage by weight of phytoplankton biomass can be directly and continuously used as a feedstock to make a fatty acid product.3. The process of wherein the feedstock consisting of phytoplankton in water does not need to be significantly dewatered and/or dried.4. The process of wherein there is no need to extract the immobilized lipids in order to make fatty acids.5. The process of that includes a horizontal hairpin hydrolysis reactor with or without internal static mixers.6. The process of that includes a vertical pipe hydrolysis reactor with internal static mixers.7. The process of that includes a vertical hairpin hydrolysis reactor with or without internal static mixers.8. The process of that includes a cylindrical fluidized bed hydrolysis reactor.9. The process of that includes a multi-stage progressively expanding fluidized bed hydrolysis reactor.10. The process of and that includes the addition of a solid particulate fluidizing media to enable operation in a backmix mode. The fluidizing media can be an inert substance or a base/alkaline heterogeneous catalyst that serves to accelerate the hydrolysis reaction.11. The process of that includes a membrane based crossflow filtration system claim 1 , whereby the weight content of biomass in the phytoplankton slurry leaving this ...

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Номер записи: 21
07-11-2013 дата публикации

Processing biomass

Номер: US20130295624A1
Автор: Marshall Medoff, Thomas Craig Masterman
Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 22
21-11-2013 дата публикации

Method and apparatus for producing cells and fat soluble materials by cell culture

Номер: US20130309757A1
Автор: Sung-Chun Kim
Принадлежит: Individual

The present invention relates to a method and apparatus for producing cells without injury and fat-soluble materials by from cell culturing in an inexpensive and highly efficient manner. The apparatus according to the present invention comprises a culturing device 10 , a solvent device 20 , a mixing device 30 , a separation device 40 , a fractionation device 50 , a cell accommodation device 60 , and a fat-soluble material solvent accommodation device 70.

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Номер записи: 23
05-12-2013 дата публикации

Tailored Oils

Номер: US20130323382A1
Автор: Bhat Riyaz, Bond Risha, Braksmayer Diza, Franklin Scott, Marangoni Alejandro, Rakitsky Walter, Rudenko George, Somanchi Aravind, ZHAO Xinhua
Принадлежит:

Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type. 198-. (canceled)99. A natural oil or refined oil produced from a natural oil , the oil comprising 3.5% or less saturated fatty acids , and optionally comprising greater than 50% oleic acid and/or greater than 1% palmitoleic acid.100. The oil of claim 99 , having between 0.1 and 3.5% saturated fatty acids.101. The oil of claim 99 , comprising at least 90% oleic acid.102. The oil of claim 101 , comprising at least 3% polyunsaturated fatty acids.103. An oleaginous cell claim 101 , optionally comprising 23S rRNA having at least 65% nucleotide sequence identity to SEQ ID NO:76 and optionally obligately heterotrophic claim 101 , that produces an oil comprising 3.5% or less saturated fatty acids.104Prototheca.. The cell of claim 103 , wherein the cell is of the genus105. The cell of claim 103 , further comprising a FATA knockout or knockdown.106. The cell of claim 103 , comprising an exogenous gene encoding an enzyme active to desaturate palmitoyl-CoA to plamitoyl-CoA.107. The cell of claim 106 , wherein the exogenous gene is a PAD gene.108. The cell of claim 106 , wherein the exogenous gene is a SAD gene having desaturase activity toward palmitoyl-ACP.109. The cell of claim 103 , further comprising an overexpressed KASII ...

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Номер записи: 24
12-12-2013 дата публикации

COMPOSITIONS COMPRISING 20-CARBON FATTY ACIDS AND METHODS OF MAKING AND USING SAME

Номер: US20130331448A1
Автор: Climax John, Manku Mehar, Rowe Jonathan
Принадлежит: DIGNITY SCIENCES LIMITED

The present disclosure provides a process for producing 20-carbon fatty acids from a plant seed oil, the process comprising: a) providing a plant seed oil comprising fatty acids, wherein the fatty acids include 18-carbon fatty acids; and b) elongating the fatty acids by two carbon atoms to produce a composition comprising 20-carbon fatty acids. 1. A process for producing 20-carbon fatty acids from a plant seed oil comprising 18-carbon fatty acids , the process comprising elongating at least a portion of the 18-carbon fatty acids present in the plant seed oil by two carbon atoms to produce 20-carbon fatty acids.2. The process of wherein said elongating step comprises contacting the 18-carbon fatty acids with an enzyme.3. The process of wherein the enzyme comprises elongase.4. The process of further comprising a step of esterifying at least a portion of the 20-carbon fatty acids.5. The process of claim 1 , wherein the 18-carbon fatty acids include a plurality of different 18-carbon fatty acids.6. The process of claim 5 , wherein the 18-carbon fatty acids comprise one or more of SDA claim 5 , GLA claim 5 , and ALA claim 5 , and the 20-carbon fatty acids comprise one or more of ETA claim 5 , ETE and DGLA.7. The process of further comprising a step of extracting the 18-carbon fatty acids from the plant seed oil prior to the elongating step.8. The process of wherein the 18-carbon fatty acids are extracted from the plant seed oil by organic solvent extraction or supercritical fluid extraction.9. The process of wherein the supercritical fluid extraction comprises COsupercritical fluid extraction (CO-SFE).10. The process of further comprising purifying the 20-carbon fatty acids claim 9 , optionally by urea fractionization claim 9 , low-temperature crystallization claim 9 , chromatographic separation claim 9 , HPLC claim 9 , or distillation.11. The process of claim 10 , wherein the 20-carbon fatty acids are purified to produce 20-carbon fatty acids having a purity selected ...

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Номер записи: 25
16-01-2014 дата публикации

BIOREFINERY SYSTEM, METHODS AND COMPOSITIONS THEREOF

Номер: US20140013658A1
Автор: Lee Sungwon, Mendez Michael, Nguyen Luan, Resnick Sol M., Saville Renee, Silverman Joshua
Принадлежит:

The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a Cmetabolizing microorganism reactor system for converting Csubstrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like. 125.-. (canceled)26. A method for making fuel , comprising converting biomass from a culture primarily comprising a Cmetabolizing non-photosynthetic microorganism into an oil composition and refining the oil composition into a fuel.27. The method according to claim 26 , wherein the biomass is converted into an oil composition by extraction.28. The method according to claim 26 , wherein the oil composition is refined by a process of cracking claim 26 , transesterification claim 26 , reforming claim 26 , distilling claim 26 , hydroprocessing claim 26 , isomerization claim 26 , or a combination thereof.29. The method according to claim 26 , wherein the fuel comprises jet fuel claim 26 , diesel fuel claim 26 , paraffinic kerosene claim 26 , gasoline claim 26 , or any combination thereof.30. The method according to claim 26 , wherein the Cmetabolizing non-photosynthetic microorganism is a bacteria or a yeast.31. The method according to claim 30 , wherein the Cmetabolizing bacteria is a methanotroph or methylotroph.32MethylomonasMethylosinus trichosporiumMethylosinus sporiumMethylocystis parvusMethylomonas methanicaMethylomonas albusMethylobacter capsulatusMethylococcus capsulatusMethylobacterium organophilumMethylomonasMethylomicrobium alcaliphilum, Methylocella silvestris, Methylacidiphilum infernorum, Methylibium petroleiphilum, Methylobacterium extorquens, Methylobacterium radiotolerans, Methylobacterium populi, Methylobacterium chloromethanicum, Methylobacterium nodulans. The method according to claim 30 , wherein the bacteria is a sp. 16a (ATCC PTA 2402) claim 30 , OB3b (NRRL B-11 claim 30 ,196) claim 30 , (NRRL B-11 claim 30 ,197) claim 30 , (NRRL B-11 claim 30 ,198) claim 30 , ( ...

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Номер записи: 26
06-02-2014 дата публикации

GENETICALLY ENGINEERED MICROBES AND USES THEREOF

Номер: US20140039232A1
Автор: Lun Desmond Siu Men
Принадлежит:

This invention concerns methods of identifying genetic alterations with which a microbe can be used to produce fatty acids at a large amount for making biofuels. Also disclosed are microbes with such genetic alterations and uses thereof. 2. A method for identifying a target for a genetic alteration claim 1 , comprising obtaining one or more genes using the model constructed by the method of claim 1 , wherein an engineered microbe having the genetic alteration produces a fatty acid at a level higher than a control microbe that is identical to the engineered microbe except that the control microbe lacks the genetic alteration.3E. coli.. The method of claim 1 , wherein the microbe is4. The method of claim 1 , wherein the model is an iAF1260 model.5. The method of claim 1 , wherein the biological optimal flux distribution is obtained by a Genetic Design through Local Search (GDLS).6. The method of claim 1 , wherein the genetic alteration is an alteration of two or more genes.7. The method of claim 1 , wherein the genetic alteration is a knockout of a gene.8. A machine-readable medium for carrying out the method of claim 1 , comprising machine-readable instructions encoded thereon which claim 1 , when executed by a processor claim 1 , cause a machine having or linked to the processor to execute the method.10. A computer system comprising the machine-readable medium of claim 8 , and a user interface capable of receiving data and displaying the record.11. An isolated cell claim 8 , that lacks a functional gene claim 8 , wherein the gene is selected from the group consisting of fadE claim 8 , rpe claim 8 , sgcE claim 8 , talA claim 8 , and talB claim 8 , and a homologue thereof.12. The isolated cell of claim 11 , wherein the cell lacks functional fadE gene.13. The isolated cell claim 11 , of claim 11 , wherein the cell lacks one or more of the functional rpe claim 11 , sgcE claim 11 , talA claim 11 , and talB genes.14E. coli. The isolated cell of claim 11 , wherein the cell ...

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Номер записи: 27
20-02-2014 дата публикации

Micoorganisms and Methods for the Production of Fatty Acids and Fatty Acid Derived Products

Номер: US20140051136A1
Автор: Evans Ron, Liao Hans, Lipscomb Tanya E.W., Louie Michael, Lynch Michael D., Prather Brittany, Ribble Wendy, Spindler Eileen, Warner Joseph R.
Принадлежит: OPX Biotechnologies, Inc.

This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism 1. A genetically modified microorganism producing a fatty acid or fatty acid derived product , wherein the fatty acid chain length can be selected from 4 to >18 , wherein fatty acids are produced via a growing fatty acyl-CoA chain and wherein additionally , the fatty acid chain is extended with at least one malonyl-CoA molecule and without the use of malonyl-ACP.2. A genetically modified microorganism of claim 1 , wherein the butyryl-CoA product/intermediate is made from acetyl-CoA and malonyl-CoA.3. A genetically modified microorganism of claim 1 , wherein the butyryl-CoA product/intermediate is made from malonyl-CoA.4. A genetically modified microorganism of claim 1 , wherein an acyl-CoA (length n) product/intermediate with chain length greater than 4 is made from acetyl-CoA and an acyl-CoA chain (length n−2).5. A genetically modified microorganism of claim 1 , wherein an acyl-CoA (length n) product/intermediate with chain length greater than 4 is made from malonyl-CoA and an acyl-CoA chain (length n−2).6. A genetically modified microorganism of claim 1 , wherein the product is a fatty acid mixture enriched for chain length C4 and wherein the C4 chain comprises >9% claim 1 , >50% claim 1 , >85% or >95% percentage of the total fatty acid product on mass (g) basis.7. A genetically modified microorganism of claim 1 , wherein the product is a fatty acid mixture enriched for chain length C6 and wherein the C6 chain comprises >10% claim 1 , >50% claim 1 , >85% or >95% percentage of the total fatty acid product on mass (g) basis.8. A genetically modified microorganism of claim 1 , wherein the product is a fatty acid ...

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Номер записи: 28
03-04-2014 дата публикации

BACTERIA AND METHOD FOR SYNTHESIZING FATTY ACIDS

Номер: US20140093921A1
Автор: Li Mai, San Ka-Yiu, Zhang Xiujun
Принадлежит: William Marsh Rice University

There is provided a recombinant bacterium comprising at least one overexpressed acyl-ACP thioesterase gene, and wherein at least one gene from the tricarboxylic acid cycle or glycolysis or both is inactivated. There is also provided a method for producing fatty acids, said method comprising culturing bacteria comprising at least one overexpressed acyl-ACP thioesterase gene in a growth medium in a container having walls; allowing said bacteria to secrete fatty acids; and collecting said fatty acids. Acid supplementation is also shown to increase productivity. 1. A recombinant microorganism comprising at least one overexpressed acyl-ACP thioesterase , and wherein i) at least one protein from the tricarboxylic acid cycle is reduced , or ii) at least one protein from glycolysis is reduced , or both i) and ii) are reduced.2. The microorganism of claim 1 , wherein said at least one protein from the tricarboxylic acid cycle is selected from the group consisting of aconitase claim 1 , isocitrate dehydrogenase claim 1 , α-ketoglutarate dehydrogenase claim 1 , succinyl-coA synthetase claim 1 , succinic dehydrogenase claim 1 , fumarase claim 1 , malate dehydrogenase claim 1 , and citrate synthase.3. The microorganism of claim 1 , wherein said at least one protein from the tricarboxylic acid cycle is succinyl-CoA synthetase.4. The microorganism of claim 1 , wherein said at least one protein from glycolysis is selected from glucokinase claim 1 , phosphoglucose isomerase claim 1 , phosphofructokinase claim 1 , aldolase claim 1 , triose phosphate isomerase claim 1 , glyceraldehyde-3-phosphate dehydrogenase claim 1 , phophoglycerate kinase claim 1 , phophoglycerate mutate claim 1 , enolase claim 1 , pyruvate kinase claim 1 , and glucose phophotransferase.5. The microorganism of claim 1 , wherein said at least one gene from glycolysis is glucokinase or glucose phophotransferase.6. The microorganism of claim 1 , further comprising at least one further modification selected from the ...

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Номер записи: 29
07-01-2016 дата публикации

Method of increasing lipid accumulation in metschnikowia pulcherrima cells

Номер: US20160002679A1
Автор: Christopher CHUCK, Fabio SANTOMAURO, Roderick Scott
Принадлежит: University of Bath

The invention relates to a method of increasing lipid accumulation in Metschnikowia pulcherrima ( Candida pulcherrima ) cells. In particular, the invention relates to a method of obtaining oil from yeast pulcherimma cells. The invention further relates to an oil and the use of pulcherimma cells for production of oleaginous biomass.

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Номер записи: 30
07-01-2016 дата публикации

ACP-MEDIATED PRODUCTION OF FATTY ACID DERIVATIVES

Номер: US20160002681A1
Автор: da Costa Bernardo, HELMAN Noah, Holden Kevin, Popova Emanuela, Rude Mathew, Simpson David, TRINH Na, VENKITESWARAN Sankaranarayanan
Принадлежит: REG Life Sciences, LLC

The disclosure relates to recombinant microorganisms that exhibit an increased expression of an acyl carrier protein (ACP) resulting in production of fatty acid derivatives. The disclosure further relates to methods of using the recombinant microorganisms in fermentation cultures in order to produce fatty acid derivatives and related compositions. 1. A recombinant host cell , comprising:(a) a polynucleotide sequence encoding an exogenous acyl carrier protein (ACP); and(b) a polynucleotide sequence encoding an exogenous fatty acid derivative biosynthetic protein, wherein the recombinant host cell produces a fatty acid derivative composition.2. The recombinant host cell of claim 1 , wherein said recombinant host cell produces said fatty acid derivative composition with a higher titer claim 1 , a higher yield or a higher productivity when cultured in medium containing a carbon source under conditions effective to overexpress said polynucleotide sequence of (a) and (b) claim 1 , as compared to a corresponding wild type host cell propagated under the same conditions as the recombinant host cell.3. The recombinant host cell of claim 1 , wherein the fatty acid derivative composition comprises a fatty acid derivative selected from the group consisting of a fatty acid claim 1 , a fatty alcohol claim 1 , a fatty ester claim 1 , a fatty aldehyde claim 1 , an alkane claim 1 , an alkene claim 1 , an olefin claim 1 , and a ketone.4. The recombinant host cell of claim 1 , wherein the fatty acid derivative biosynthetic protein has thioesterase activity and the fatty acid derivative composition comprises a fatty acid.5. The recombinant host cell of claim 4 , further comprising a protein that has carboxylic acid reductase (CAR) activity claim 4 , wherein the fatty acid derivative composition comprises a fatty alcohol.6. The recombinant host cell of claim 1 , wherein the fatty acid derivative biosynthetic protein has acyl ACP reductase (AAR) activity and the fatty acid derivative ...

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Номер записи: 31
07-01-2016 дата публикации

Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same

Номер: US20160002683A1
Автор: Lars Henrik Oestergaard, Lisbeth Kalum, Sara Landvik
Принадлежит: Novozymes AS

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 32
05-01-2017 дата публикации

PROCESSES FOR FERMENTATION OF LIGNOCELLULOSIC GLUCOSE TO ALIPHATIC ALCOHOLS OR ACIDS

Номер: US20170002387A1
Автор: PYLKKANEN Vesa, RETSINA Theodora
Принадлежит:

A process for producing an organic aliphatic product (such as butanol) from lignocellulosic biomass is provided, comprising: (a) fractionating lignocellulosic biomass in the presence of a solvent for lignin, a hydrolysis catalyst, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin; (b) washing the cellulose-rich solids and separating the cellulose-rich solids from the liquor; (c) enzymatically hydrolyzing the cellulose-rich solids to generate a hydrolysate comprising glucose; (d) detoxifying the hydrolysate by neutralizing the hydrolysate, removing insoluble solids, and removing or oxidizing residual hydrolysis catalyst, thereby generating a purified hydrolysate; (e) fermenting the purified hydrolysate using a suitable microorganism to produce a dilute organic aliphatic product, wherein the microorganism is recycled with a membrane; (f) extracting the dilute organic aliphatic product into a water-immiscible extractant, to generate an intermediate material; and (g) distilling the intermediate material to generate a concentrated organic aliphatic product. 1. A process for producing an organic aliphatic product from lignocellulosic biomass , said process comprising:(a) fractionating a feedstock comprising lignocellulosic biomass in the presence of a solvent for lignin, a hydrolysis catalyst, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin;(b) washing said cellulose-rich solids and separating said cellulose-rich solids from said liquor;(c) enzymatically hydrolyzing said cellulose-rich solids to generate a hydrolysate comprising glucose;(d) detoxifying said hydrolysate by neutralizing said hydrolysate, removing insoluble solids, and removing or oxidizing residual hydrolysis catalyst, thereby generating a purified hydrolysate;(e) fermenting said purified hydrolysate using a suitable microorganism in a fermentor to produce a dilute organic aliphatic product, wherein said microorganism is ...

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Номер записи: 33
04-01-2018 дата публикации

Methods, reagents and cells for biosynthesizing compounds

Номер: US20180002729A1
Автор: Adriana Leonora Botes, Alex van Eck CONRADIE, Ramdane Haddouche
Принадлежит: Invista North America LLC

This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol.

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Номер записи: 34
03-01-2019 дата публикации

TAILORED OILS

Номер: US20190002934A1
Автор: Bhat Riyaz, Franklin Scott, Moseley Jeffrey L., Rudenko George, Somanchi Aravind, ZHAO Xinhua
Принадлежит:

Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type. 184.-. (canceled)85ProtothecaChlorella,. A recombinant cell of the genus or said recombinant cell comprising a knockout or knockdown of an endogenous Fatty acyl-ACP thioesterase gene , and further comprising exogenous nucleic acids that encode lysophosphatidic acid acyltransferase (LPAAT).86. The recombinant cell of claim 85 , wherein said exogenous nucleic acids that encode LPAAT encodes an LPPAT having at least 90% amino acid sequence identity to an LPAAT selected from the group consisting of SEQ ID NOs: 16 claim 85 , 77 claim 85 , 78 claim 85 , 79 and 157.87. The recombinant cell of claim 86 , wherein said exogenous nucleic acids that encode LPAAT encodes an LPPAT having at least 95% amino acid sequence identity to an LPAAT selected from the group consisting of SEQ ID NOs: 16 claim 86 , 77 claim 86 , 78 claim 86 , 79 and 157.88. The recombinant cell of claim of claim 86 , wherein said exogenous nucleic acids encodes an LPAAT comprising the amino acid sequences of SEQ ID NO: 157.89. The recombinant cell of claim of claim 86 , wherein said exogenous nucleic acids encodes an LPAAT comprising the amino acid sequences of SEQ ID NOs: 17 claim 86 , 77 claim 86 , 78 or 79.90. The recombinant cell of claim 89 , wherein ...

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Номер записи: 35
14-01-2016 дата публикации

METHODS AND SYSTEMS FOR THE SIMULTANEOUS PRODUCTION OF LIPIDS AND AROMATICS FROM CELLULOSE FEEDSTOCKS

Номер: US20160010125A1
Автор: Alisala Kashinatham, Guidi Sara, Menon Suresh M., Newman David E., Orchard Samantha, Sircar Jagadish Chandra, Yang Kay A.
Принадлежит:

A system and method are provided which utilize microbes to convert biomass feedstock into a fuel. In one aspect, a method of producing lipids includes receiving a feedstock including biomass, exposing the feedstock to microbes which are capable of converting the feedstock into lipids, and extracting produced lipids. 130-. (canceled)31. A method for producing lipids comprising:(a) receiving a feedstock including biological matter;(b) inoculating the feedstock with microbes selected from the group consisting of bacterial species, fungal species or a combination thereof, wherein the microbes convert cellulose, hemicellulose or glycerol into lipids upon fermentation with the feedstock;(c) fermenting the inoculated feedstock, thereby producing lipids.32Trichoderma reesi, AcinetobacterActinomycesStreptomyces. The method of claim 31 , wherein the microbes comprise sp. claim 31 , or members of the and genera.33. The method of claim 31 , wherein the feedstock comprises cellulose claim 31 , hemicellulose or a combination thereof.34. The method of claim 33 , wherein the feedstock comprises sawdust claim 33 , wood chips claim 33 , algae claim 33 , or municipal solid waste.35. The method of claim 31 , wherein the feedstock is fortified with glycerol.36. The method of claim 31 , further comprising pretreating the feedstock with at least one of mechanical pretreatment claim 31 , thermal-chemical pretreatment claim 31 , sterilization claim 31 , ultraviolet irradiation claim 31 , pasteurization claim 31 , filtration and separation prior to inoculating the feedstock.37. The method of claim 36 , wherein the pretreatment makes the feedstock more amenable to microbial digestion.38. The method of claim 36 , wherein the wherein the pretreatment increases the ratio of surface area to volume of the feedstock.39. The method of claim 36 , wherein the mechanical pretreatment comprises chopping claim 36 , shredding claim 36 , grinding or a combination thereof.40. The method of claim 36 , ...

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Номер записи: 36
11-01-2018 дата публикации

PRODUCTION OF FATTY ACID DERIVATIVES

Номер: US20180010137A1
Автор: Clarke Elizabeth J., Da Costa Bernardo M., Greenfield Derek L., Groban Eli S., HELMAN Noah, Holden Kevin, HU Zhihao, Schirmer Andreas W.
Принадлежит:

The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell. 151.-. (canceled)52. A recombinant host cell comprising a decreased activity of a phosphoenolpyruvate carboxylase (ppc) polypeptide , wherein said recombinant host cell produces a fatty acid derivative composition at a higher titer , yield or productivity than a corresponding wild type host cell when cultured in a medium containing a carbon source under conditions effective to decrease expression of said ppc polypeptide.53. A cell culture comprising the recombinant host cell according to .54. The cell culture of claim 52 , wherein said cell culture comprises a fatty acid derivative composition.55. The cell culture of claim 54 , wherein the fatty acid derivative composition comprises at least one fatty acid derivative selected from the group consisting of fatty acid claim 54 , a fatty ester claim 54 , a fatty alcohol claim 54 , a fatty aldehyde claim 54 , an alkane claim 54 , a terminal olefin claim 54 , an internal olefin claim 54 , and a ketone.56. The cell culture of claim 53 , wherein the fatty acid derivative isa) a C6, C8, C10, C12, C13, C14, C15, C16, C17, or C18 fatty acid derivative, orb) a C10:1, C12:1, C14:1, C16:1, or C18:1 unsaturated fatty acid derivative.57. The cell culture of claim 54 , wherein the fatty acid derivative composition comprises:a) one or more of C8, C10, C12, C14, C16, and C18 fatty acid derivatives,b) fatty acids,c) fatty aldehydes,d) fatty alcohols,e) fatty esters,f) alkanes,g) terminal olefins,h) internal olefins, ori) ...

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Номер записи: 37
09-01-2020 дата публикации

ACYL-ACP REDUCTASE WITH IMPROVED PROPERTIES

Номер: US20200010813A1
Автор: Gano Jacob, Rude Mathew, Schirmer Andreas, TRINH Na
Принадлежит:

The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics. 182.-. (canceled)83. A variant acyl-ACP reductase (AAR) polypeptide comprising at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 80 , wherein said variant AAR polypeptide comprises a mutation at amino acid position selected from the group consisting of Q40V , G52V , G273E , K303G , H340P , L344A , L344D , L344S , L344T , A345R , V346P , V346G , and A345* , and wherein said AAR polypeptide catalyzes the conversion of an acyl-ACP to a fatty aldehyde.84. The variant AAR polypeptide of claim 83 , wherein expression of the variant AAR polypeptide in a recombinant host cell results in a higher titer of a fatty aldehyde or fatty alcohol composition as compared to a titer of a fatty aldehyde or fatty alcohol composition produced by expression of a wild type AAR polypeptide in a corresponding wild type host cell.85. The variant AAR polypeptide of claim 83 , wherein said fatty alcohol composition is a C12 claim 83 , C14 or C16 fatty alcohol composition claim 83 , or a combination thereof.86. A recombinant host cell expressing the variant AAR polypeptide of .87. The recombinant host cell of claim 86 , wherein the recombinant host cell produces a fatty aldehyde or fatty alcohol composition with a titer that is at least 10% greater claim 86 , at least 15% greater claim 86 , at least 20% greater claim 86 , at least 25% greater claim 86 , or at least 30% greater than the titer of a fatty aldehyde or alcohol composition produced by a host cell expressing a corresponding wild type AAR polypeptide claim 86 , when cultured in medium containing a carbon source under conditions effective to express the variant AAR polypeptide.88. ...

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Номер записи: 38
09-01-2020 дата публикации

LONG-CHAIN DIBASIC ACID WITH LOW CONTENT OF FATTY ACID IMPURITY AND A METHOD OF PRODUCING THE SAME

Номер: US20200010862A1
Автор: Chou Howard, Liu Wenbo, LIU Xiucai, XU Min, Yang Chen
Принадлежит:

The invention relates to a long-chain dibasic acid with low content of fatty acid impurity and a method of producing it, in particular to the preparation of a long-chain dibasic acid producing strain by using directed evolution and homologous recombination, and to the fermentation production of a long-chain dibasic acid with low content of fatty acid impurity by using said strain. The invention relates to an isolated mutated CPR-b gene, homologous gene or variant thereof, relative to the GenBank Accession Number AY823228, taking the first base upstream of the start codon ATG as −1, comprising a base mutation −322G>A, and taking the first base downstream of the stop codon TAG as 1, comprising mutations 3TUTR.19C>T and 3′UTR.76_77insT. The invention also relates to a strain containing said mutated CPR-b gene, homologous gene or variant thereof, wherein, when the strain is used to produce a long-chain dibasic acid by fermentation, the content of fatty acid impurity in the fermentation product is significantly decreased. 1. A product , which is one of the following products I) to IV):I) an isolated mutated CPR-b gene, homologous gene or variant thereof, relative to the GenBank Accession Number AY823228, taking the first base upstream of the start codon ATG as −1, comprising a base mutation −322G>A, and taking the first base downstream of the stop codon TAG as 1, comprising mutations 3TUTR.19C>T and 3′UTR.76_77insT; wherein the variant has at least 70% sequence identity to the mutated CPR-b gene or homologous gene thereof;II) a microorganism containing the mutated CPR-b gene, homologous gene or variant of I), which produces a long-chain dibasic acid with decreased content of a fatty acid impurity, compared to a microorganism containing a non-mutated CPR-b gene or homologous gene thereof;III) a long-chain dibasic acid with low content of a fatty acid impurity, wherein the content of the fatty acid impurity contained in the long-chain dibasic acid is more than 0, and less ...

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Номер записи: 39
09-01-2020 дата публикации

LONG-CHAIN DIBASIC ACID WITH LOW CONTENT OF MONOBASIC ACID IMPURITY AND THE PRODUCTION METHOD THEREOF

Номер: US20200010863A1
Автор: Chou Howard, Liu Wenbo, LIU Xiucai, XU Min, Yang Chen
Принадлежит:

The invention relates to a long-chain dibasic acid with low content of monobasic acid impurity and a production method thereof, in particular to the preparation of a long-chain dibasic acid producing strain by means of directed evolution and homologous recombination, and to the production of a long-chain dibasic acid with low content of monobasic acid impurity by fermentation of said strain. The invention relates to a mutated CYP52A12 gene, homologous gene or variant thereof, which, relative to GenBank Accession Number AY230498 and taking the first base upstream of the start codon ATG as −1, comprises a mutation. The invention relates to a strain comprising said mutated CYP52A12 gene, homologous gene or variant thereof wherein when the strain is fermented to produce a long-chain dibasic acid, the content of monobasic acid impurity in the fermentation product is significantly reduced. 1. A product , which is one of the following products I) to VI):I) an isolated DNA molecule, which, relative to nucleotides 1-1176 or 265-1176 of SEQ ID NO: 21, comprises any one or more of base mutations 301A>G, 324A>T, 346delT, 352C>A, 354delG, 598A>G, 765_766AC>TT, 774insTT and 1162_1176ACCAACCAACCAACC>ACCAACCAACC;II) an isolated mutated CYP52A12 gene, homologous gene or variant thereof, which, relative to GenBank Accession Number AY230498 and taking the first base upstream of the start codon ATG as −1, comprises any one or more of the following base mutations: −876A>G; −853A>T; −831delT; −825C>A; −823delG; −579A>G; −412_−411AC>TT; −402insTT and −15_1 ACCAACCAACCAACCA>ACCAACCAACCA, wherein the variant has at least 70% sequence identity to the mutated CYP52A12 gene or homologous gene thereof;III) a microorganism containing the isolated DNA molecule of I), which produces a long-chain dibasic acid with decreased content of monobasic acid impurity, compared to a microorganism not containing the DNA molecule;IV) a microorganism containing the mutated CYP52A12 gene, homologous gene or ...

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Номер записи: 40
03-02-2022 дата публикации

Method of Producing Lipid

Номер: US20220033865A1
Автор: OZAKI Tatsuro
Принадлежит: KAO CORPORATION

A method of producing lipids, containing the steps of: 2. A method of improving lipid productivity , comprisingintroducing a gene encoding a protein containing a thioredoxin domain and a thioredoxin reductase domain into an algal host to enhance expression of the gene, thereby improving the amount of total fatty acids produced in a cell of the algal host.3Nannochloropsis.. The method according to claim 1 , wherein the alga is an alga belonging to the genus4H. The method according to claim 1 , wherein the thioredoxin domain is a domain consisting of the following amino acid sequence (A) or (B) claim 1 , or the following amino acid sequence (C) or (D) claim 1 , and the thioredoxin reductase domain is a domain consisting of the following amino acid sequence (E) or (F) claim 1 , or the following amino acid sequence (G) or ():(A) the amino acid sequence at positions 529 to 629 of the amino acid sequence set forth in SEQ ID NO: 1;(B) an amino acid sequence having 60% or more identity with the amino acid sequence (A), and constituting the thioredoxin domain having thioredoxin activity;(C) the amino acid sequence at positions 525 to 625 of the amino acid sequence set forth in SEQ ID NO: 3;(D) an amino acid sequence having 60% or more identity with the amino acid sequence (C), and constituting the thioredoxin domain having thioredoxin activity;(E) the amino acid sequence at positions 137 to 448 of the amino acid sequence set forth in SEQ ID NO: 1;(F) an amino acid sequence having 60% or more identity with the amino acid sequence (E), and constituting the thioredoxin reductase domain having thioredoxin reductase activity;(G) the amino acid sequence at positions 134 to 445 of the amino acid sequence set forth in SEQ ID NO: 3; and{'i': 'H', '() an amino acid sequence having 60% or more identity with the amino acid sequence (G), and constituting the thioredoxin reductase domain having thioredoxin reductase activity.'}5. The method according to claim 1 , wherein the protein ...

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Номер записи: 41
15-01-2015 дата публикации

Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same

Номер: US20150017694A1
Автор: Christer Jansson, David Galgoczy, Henrik Fyrst, Itzhak Kurek, John S. Reed, Lisa Dyson, Michael Siani-Rose
Принадлежит: Kiverdi Inc

Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

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Номер записи: 42
21-01-2016 дата публикации

GENETICALLY MODIFIED MICROORGANISM FOR PRODUCING LONG-CHAIN DICARBOXYLIC ACID AND METHOD OF USING THEREOF

Номер: US20160017387A1
Автор: Chien Liang-Jung, HSIEH Hsin-Ju, Lin Yu-Ju, Wang Jia-Hung
Принадлежит:

Described herein are genetically-modified microorganisms for producing long-chain dicarboxylic acids and methods of using the microorganisms. The microorganisms contain a first nucleic acid encoding an lauroyl ACP-thioesterase (BTE) operably linked to a promoter or a second nucleic acid encoding a lauroyl ACP-thioesterase (FatB3) operably linked to a promoter.

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Номер записи: 43
18-01-2018 дата публикации

BIOMASS OF THE MICROALGAE SCHIZOCHYTRIUM MANGROVEI AND METHOD FOR PREPARING SAME

Номер: US20180016605A1
Автор: Comini Serge, Pora Bernard
Принадлежит:

The invention concerns a strain of Shizochytrium mangrovei filed on 22 Nov. 2012 with the CNCM as number 1-4702 having the ability to produce a high quantity of docosahexaenoic acid (or DHA) and palmitic acid, the methods for producing the corresponding biomass containing said lipid compounds of interest, and the biomass containing the products and compositions prepared from this strain. 111-. (canceled)12Schizochtrium mangrovei. A strain of deposited on Nov. 22 , 2012 at the CNCM under number I-4702.13Schizochtrium mangrovei. A lipid rich biomass of microalga strain deposited on Nov. 22 , 2012 at the CNCM under number 1-4702 , comprising at least one of docosahexaenoic acid (or DHA) and palmitic acid.14. The biomass according to claim 13 , comprising docosahexaenoic acid (or DHA) and palmitic acid.15. The biomass according to claim 14 , comprising between 35 and 40% of DHA claim 14 , and between 40 and 50 wt % of palmitic acid claim 14 , respectively expressed by weight of total fatty acids.16. The biomass according to claim 15 , further comprising between 1.5 and 2% of phospholipids claim 15 , expressed by weight of biomass at 99% of dry matter.17. The biomass according to claim 16 , comprising a total amino acids content of between 10 and 20% expressed as Nx6.25 claim 16 , the percentage being expressed by weight of biomass.18. A food product or supplement claim 16 , comprising the biomass according to claim 16 , for consumption by humans or animals.19. The biomass according to claim 13 , comprising claim 13 , between 35 and 40% of DHA and between 40 and 50 wt % of palmitic acid claim 13 , expressed by weight of total fatty acids claim 13 , and between 1.5 and 2% of phospholipids claim 13 , expressed by weight of biomass at 99% of dry matter.20. A food product comprising a biomass produced by the method of .21. The food product as claimed in claim 20 , wherein said biomass comprises:between 35 and 40% of DHA and between 40 and 50 wt % of palmitic acid, expressed ...

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Номер записи: 44
17-01-2019 дата публикации

PROCESSES FOR RECOVERING PRODUCTS FROM A CORN FERMENTATION MASH

Номер: US20190017080A1
Автор: BOOTSMA Jason
Принадлежит: FLINT HILLS RESOURCES, LP

Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product. 1. A process for recovering products from a fermentation mash , comprising:processing a ground corn product to produce a fermentation mash comprising ethanol;separating at least a portion of the ethanol from the fermentation mash to produce a whole stillage; andseparating the whole stillage to produce a fiber rich product and a filtrate, wherein the fiber rich product has an ethanol potential of at least 90 gallons of ethanol per 1,000 kilograms of the fiber rich product.2. The process of claim 1 , wherein the ethanol potential is 90 gallons of ethanol to about 160 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.3. The process of claim 1 , wherein the ethanol potential is 90 gallons of ethanol to about 145 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.4. The process of claim 1 , wherein the ethanol potential is about 100 gallons of ethanol to about 160 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.5. The process of claim 1 , wherein the ethanol potential is about 100 gallons of ethanol to about 150 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.6. The process of claim 1 , wherein the ethanol potential is about 100 gallons of ethanol to about 145 gallons of ethanol per 1 claim 1 ,000 kilograms of the fiber rich product.7. The process of claim 1 , ...

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Номер записи: 45
21-01-2021 дата публикации

PROCESSES FOR RECOVERING PRODUCTS FROM A CORN FERMENTATION MASH

Номер: US20210017547A1
Автор: BOOTSMA Jason
Принадлежит: FLINT HILLS RESOURCES, LP

Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product. 1. A process for recovering products from a fermentation mash , comprising:processing a ground corn product to produce a fermentation mash comprising ethanol;separating at least a portion of the ethanol from the fermentation mash to produce a whole stillage;separating the whole stillage to produce a fiber rich product and a filtrate;hydrolyzing the fiber rich product to produce a saccharification mash; andprocessing the saccharification mash to produce additional ethanol and a stillage protein product.2. The process of claim 1 , wherein the stillage protein product comprises about 15 wt % to about 35 wt % of yeast claim 1 , based on a dry weight of the stillage protein product.3. The process of claim 1 , wherein the stillage protein product comprises about 40 wt % to about 80 wt % of protein claim 1 , about 3 wt % to about 20 wt % of fat claim 1 , about 1 wt % to about 4 wt % of ash claim 1 , about 2 wt % to about 30 wt % of neutral detergent fibers claim 1 , about 1 wt % to about 15 wt % of acid detergent fibers claim 1 , and about 15 wt % to about 35 wt % of yeast claim 1 , based on a dry weight of the stillage protein product.4. The process of claim 1 , wherein the stillage protein product comprises about 47 wt % to about 57 wt % of protein claim 1 , about 3 wt % to about 5 wt % of fat claim 1 , about 1 wt % to about 3 wt % of ash claim 1 , about 4 wt ...

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Номер записи: 46
16-01-2020 дата публикации

Microbiomes and methods for producing medium-chain fatty acids from organic substrates

Номер: US20200017891A1
Автор: Daniel Noguera, Matthew Scarborough, Timothy James Donohue
Принадлежит: WISCONSIN ALUMNI RESEARCH FOUNDATION

Microbiome compositions and uses thereof. The microbiome compositions include a set of microbes. The sets of microbes contain members of Lactobacillaceae, Eubacteriaceae, Lachnospiraceae, and Coriobacteriaceae. The number of individual physical microbes in the set constitutes a certain percentage of the total number of individual physical microbes in the microbiome composition. The microbiome compositions can be used for producing medium-chain fatty acids from organic substrates through anaerobic fermentation in a medium. The medium can include lignocellulosic stillage.

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Номер записи: 47
16-01-2020 дата публикации

MUTANT YEAST STRAIN CAPABLE OF PRODUCING MEDIUM CHAIN FATTY ACIDS

Номер: US20200017892A1
Автор: ANDRE Isabelle, Barbe Sophie, BORDES Florence, Croux Christian, Daboussi Fayza, Gueroult Marc, Marty Alain, Percheron Benjamin, Rigouin Coraline
Принадлежит:

Embodiments of the present disclosure relate to mutant yeast strains, in particular mutant strains, capable of producing medium chain fatty acids compared to the parent oleaginous yeast strain from which said mutant oleaginous yeast strain derives. Embodiments of the present disclosure also relate to means and methods for obtaining these mutant yeast strains. 1. A method for increasing the ratio of fatty acids having a hydroxycarbon chain length consisting of 16 carbons (C16 fatty acids) to fatty acids having a hydroxycarbon chain consisting of 18 carbons (C18 fatty acids) and/or for increasing the amount of medium chain length fatty acids (C8-C15 fatty acids) , produced by a yeast strain , compared to the parent yeast strain from which said yeast strain derives , comprising expressing in said yeast strain a mutated fatty acid synthase subunit alpha (αFAS) , wherein the amino acid residue of said αFAS corresponding to the amino acid residue at position 1220 in SEQ ID NO: 1 is substituted with a larger steric hindrance amino acid residue.2. The method according to claim 1 , wherein:when the amino acid residue corresponding to the amino acid residue at position 1220 in SEQ ID NO: 1 of the non-mutated αFAS is isoleucine (I) then it is substituted with an amino acid residue selected from the group consisting of phenylalanine (F), histidine (H), methionine (M), tryptophan (W) and tyrosine (Y),when the amino acid residue corresponding to the amino acid residue at position 1220 in SEQ ID NO: 1 of the non-mutated αFAS is valine (V) then it is substituted with an amino acid residue selected from the group consisting of isoleucine (I) phenylalanine (F), histidine (H), methionine (M), tryptophan (W) and tyrosine (Y), andwhen the amino acid residue corresponding to the amino acid residue at position 1220 in SEQ ID NO: 1 of the non-mutated αFAS is methionine (M) then it is substituted with an amino acid residue selected from the group consisting of phenylalanine (F), histidine ( ...

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Номер записи: 48
17-04-2014 дата публикации

Enhanced Fermentation From Pretreatment Products

Номер: US20140106418A1
Автор: Benjamin STAUBER, Carl P. FELICE, Sarad Parekh
Принадлежит: Sweetwater Energy Inc

Provided are methods and compositions for higher yields through the addition of pretreatment/hydrolysis extracts in the fermentation process. These fractions increase the rate of growth for biocatalysts and result in improved composition of fermentation end products.

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Номер записи: 49
26-01-2017 дата публикации

METHOD FOR PRODUCING RARE FATTY ACID USING NOVEL ENZYME, AND NOVEL RARE FATTY ACID

Номер: US20170022526A1
Автор: Goto Tsuyoshi, KAWADA Teruo, KISHINO Shigenobu, Ogawa Jun, Takahashi Nobuyuki, YONEJIMA Yasunori
Принадлежит:

The present invention provides production of hydroxylated fatty acid by a hydration reaction using a novel enzyme derived from and using fatty acid as a substrate, and further, a production method of oxo fatty acid by an enzyme reaction or chemical oxidation reaction using the hydroxylated fatty acid as a substrate. In addition, a valuable novel rare fatty acid obtained by such production method is also provided. 1. An enzyme protein of any of the following (a)-(c):(a) the enzyme protein consisting of the amino acid sequence shown in SEQ ID NO: 2,(b) a protein comprising an amino acid sequence wherein one or plural amino acids in the amino acid sequence shown in SEQ ID NO: 2 are deleted and/or substituted and/or inserted and/or added, and having an enzyme activity that catalyzes a hydration reaction,(c) a protein encoded by a base sequence that hybridizes to a nucleic acid consisting of a chain sequence complementary to the base sequence shown in SEQ ID NO: 1 under stringent conditions, and having an enzyme activity that catalyzes a hydration reaction.2Lactobacillus acidophilus. fungus or fungal debris thereof claim 1 , comprising the enzyme protein according to .3. A nucleic acid encoding the enzyme protein according to .4. A vector comprising the nucleic acid according to .5. A host cell transformed with the vector according to .6. A method of producing the enzyme protein according to claim 4 , comprising culturing the host cell according to and recovering the enzyme from the culture.7. A method of producing a hydroxylated fatty acid having 18 carbon atoms and a hydroxyl group at the 13-position claim 1 , comprising subjecting an unsaturated fatty acid having 18 carbon atoms and a cis double bond at the 12-position to a hydration reaction using the enzyme protein according to claim 1 , wherein the unsaturated fatty acid having 18 carbon atoms and a cis double bond at the 12-position is linoleic acid claim 1 , γ-linolenic acid claim 1 , α-linolenic acid claim 1 , ...

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Номер записи: 50
23-01-2020 дата публикации

PROCESS OF OMEGA-HYDROXYL FATTY ACID PRODUCTION

Номер: US20200024624A1
Автор: Cao Dong, Haas Thomas, Li Li, Pötter Markus, TAO Guoqing
Принадлежит: EVONIK DEGUSSA GmbH

Provided is a method of producing at least one omega-hydroxyl fatty acid, the method comprising: (a) contacting at least one alkane with at least one recombinant yeast cell in an aqueous medium, wherein the yeast cell is capable of oxidising the alkane to the corresponding omega-hydroxyl fatty acid and the yeast cell comprises a reduced fatty acid degradation capacity. 1: A method of producing at least one omega-hydroxyl fatty acid , the method comprising:contacting at least one alkane with at least one yeast cell in an aqueous medium,wherein the at least one yeast cell is capable of oxidizing the at least one alkane to a corresponding omega-hydroxyl fatty acid and the at least one yeast cell comprises a reduced fatty acid degradation capacity;the reduced fatty acid degradation capacity is a result of:(i) a decrease in expression relative to a wild type cell of at least one enzyme involved in a beta-oxidation pathway; and/or(ii) at least one loss-of-function mutation in at least one enzyme involved in the beta-oxidation pathway; andthe aqueous medium comprises acetate as a co-substrate.2: The method according to claim 1 , wherein an acetate concentration is at least 20 mmol/L.3: The method according to claim 1 , wherein an acetate concentration is maintained between 80-800 mmol/L.4: The method according to claim 1 , wherein an acetate concentration is maintained between 160-500 mmol/L.5: The method according to claim 1 , wherein the aqueous medium further comprises a non-detectable concentration of glucose.6: The method according to claim 1 , wherein the acetate is selected from the group consisting of NaAc and HAc.7: The method according to claim 1 , wherein the at least one alkane comprises at least 6 carbon atoms.8: The method according to claim 1 , wherein the at least one alkane is selected from the group consisting of C-Calkanes.9: The method according to claim 1 , wherein the at least one alkane is selected from the group consisting of C-Calkanes.10Candida, ...

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Номер записи: 51
28-01-2021 дата публикации

SOLID-SOLID SEPARATION PROCESS FOR LONG CHAIN DICARBOZYLIC ACIDS

Номер: US20210024446A1
Автор: LIU Xiucai, Qin Bingbing, Yang Yufeng
Принадлежит:

The present disclosure provides method for isolating a long chain dicarboxylic acid such as a substantially pure or pure long chain dicarboxylic acid from a fermentation broth containing microbial cells. 1. A method for isolating a long chain dicarboxylic acid from a fermentation broth containing microbial cells , wherein the long chain dicarboxylic acid is a saturated or unsaturated straight chain dicarboxylic acid having 9 to 18 carbon atoms , with a carboxyl group at two ends of the chain , the method comprising:(a) adding an acid to the fermentation broth to produce a demulsified fermentation broth having a pH of 2-5 or 2.5-4;(b) centrifuging the demulsified fermentation broth using a hydraulic cyclone centrifuge, a hydrocyclone centrifuge, a horizontal spiral centrifuge, or any combination thereof to generate a first phase comprising the long chain dicarboxylic acid and a second phase comprising the microbial cells; and(c) isolating the long chain dicarboxylic acid from the first phase by filtration to recover an isolated long chain dicarboxylic acid product.2. The method of claim 1 , wherein the first phase is substantially free of the microbial cells.3. The method of claim 1 , wherein the second phase is substantially free of the long chain dicarboxylic acid.4. The method of claim 1 , wherein the fermentation broth is a sterilized. fermentation broth.5. The method of claim 1 , wherein the fermentation broth is a diluted fermentation broth.6. The method of claim 5 , wherein the diluted fermentation broth includes at least 0.5% claim 5 , or about 0.5% to about 10% microbial cells (dry weight) claim 5 , e.g. 0.5% or 0.6% microbial cells (dry weight).7. The method of claim 1 , wherein the total concentration of dicarboxylic acids in the fermentation broth is at least about 85% (dry weight) claim 1 , e.g. claim 1 , about 85% claim 1 , about 86% claim 1 , about 87% claim 1 , about 88% claim 1 , about 89% claim 1 , about 90% claim 1 , about 91% claim 1 , about 92% ...

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Номер записи: 52
28-01-2021 дата публикации

BIOTECHNOLOGICAL PRODUCTION OF ALCOHOLS AND DERIVATIVES THEREOF

Номер: US20210024967A1
Автор: Engel Philip, Gehring Christian, Haas Thomas, Pfeffer Jan Christoph, Poetter Markus, Thum Oliver
Принадлежит: EVONIK OPERATIONS GMBH

A method for oxidizing an alkyl, including a) contacting the alkyl with an aqueous solution comprising a microorganism where the microorganism has a reduced fatty acid degradation capacity compared to its wild type, wherein the fatty acid degradation capacity is reduced by deletion, inhibition or inactivation of a gene encoding an enzyme involved in the β-oxidation pathway; and the microorganism expresses a recombinant alkane oxidase, and b) contacting the aqueous solution from a) with a water-immiscible organic solvent. 1: A method for oxidizing an alkyl , comprisinga) contacting the alkyl with an aqueous solution comprising a microorganism where the microorganism has a reduced fatty acid degradation capacity compared to its wild type, wherein the fatty acid degradation capacity is reduced by deletion, inhibition or inactivation of a gene encoding an enzyme involved in the β-oxidation pathway; and the microorganism expresses a recombinant alkane oxidase, andb) contacting the aqueous solution from a) with a water-immiscible organic solvent,{'sub': 2', 'x', '2', '2', '3', '3, 'sup': 1', '1, 'wherein the alkyl is a compound represented by the formula H—(CH)—R, wherein x is at least 8, and R is selected from the group comprising —OH, —COH, —COOH, —COOR, —NH, —NO, —CN, —OPOH, —SOH and —H wherein Ris methyl or ethyl, and'}{'i': 'Pseudomonas putida', 'wherein the expressed recombinant alkane oxidase is AlkB from GPo1 comprising the sequence of SEQ ID NO: 1 or a variant thereof having at least 90% identity to SEQ ID NO: 1, and'} a fatty acid importer comprising the sequence of SEQ ID NO: 2 or a variant thereof having at least 90% identity to SEQ ID NO: 2,', 'a fatty acid-CoA ligase comprising the sequence of SEQ ID NO: 3 or a variant thereof having at least 90% identity to SEQ ID NO: 3,', 'an acyl-CoA dehydrogenase comprising the sequence of SEQ ID NO: 4 or a variant thereof having at least 90% identity to SEQ ID NO: 4,', 'an enoyl-CoA hydratase comprising the sequence of ...

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Номер записи: 53
29-01-2015 дата публикации

Control of phytopathogenic microorganisms with pseudomonas sp. and substances and compositions derived therefrom

Номер: US20150030577A1
Автор: Ana Lucia Cordova-Kreylos, Carly TODD, Ratnakar Asolkar
Принадлежит: Marrone Bio Innovations Inc

Provided are compounds and compositions derived from Pseudomonas sp., particularly, Pseudomonas fluorescens or Pseudomonas protegens and more particularly strain having the identifying characteristics of Pseudomonas ATCC 55799 having antimicrobial properties and particularly, antibacterial properties.

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Номер записи: 54
02-02-2017 дата публикации

Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same

Номер: US20170029787A1
Автор: Kalum Lisbeth, Landvik Sara, Oestergaard Lars Henrik
Принадлежит: NOVOZYMES A/S

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 129-. (canceled).30. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted , linear or branched , alkane or saturated fatty acid having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 , comprising contacting the alkane or saturated fatty acid with hydrogen peroxide and a polypeptide having peroxygenase activity and at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2 , wherein the alkane or saturated fatty acid is optionally substituted with one or two substituents selected from the group consisting of halogen , hydroxyl , carboxyl , amino , nitro , cyano , thiol , sulphonyl , formyl , acetyl , methoxy , ethoxy , phenyl , benzyl , xylyl , carbamoyl and sulfamoyl.31. The method of claim 30 , wherein an alkane is hydroxylated.32. The method of claim 31 , wherein the alkane is pentane claim 31 , hexane claim 31 , heptane claim 31 , octane claim 31 , nonane claim 31 , decane claim 31 , undecane claim 31 , dodecane claim 31 , tridecane claim 31 , tetradecane claim 31 , pentadecane or hexadecane claim 31 , or an isomer thereof.33. The method of claim 31 , wherein the alkane is unsubstituted.34. The method of claim 31 , wherein the alkane is linear.35. The method of claim 31 , wherein the alkane is converted to a diol by introduction of two hydroxy groups.36. The method of claim 30 , wherein a saturated fatty acid is hydroxylated.37. The method of claim 36 , wherein the saturated fatty acid is selected from the group consisting of butanoic acid claim 36 , pentanoic acid claim 36 , hexanoic acid claim 36 , heptanoic acid claim 36 , octanoic acid claim 36 , nonanoic acid claim 36 , ...

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Номер записи: 55
01-02-2018 дата публикации

BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID

Номер: US20180030486A1
Автор: Beardslee Tom, Eirich L. Dudley, LaPlaza Jose Miguel, PICATAGGIO Stephen
Принадлежит:

The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production. 1168-. (canceled)169. A method for producing a diacid , comprising:contacting a genetically modified yeast with a feedstock capable of being converted by the yeast to a diacid; andculturing the yeast under conditions in which the diacid is produced from the feedstock,wherein the genetically modified yeast comprises: one or more genetic modifications that substantially block beta oxidation activity; and one or more genetic modifications that increase ADH2a alcohol dehydrogenase activity, wherein ADH2a alcohol dehydrogenase comprises the amino acid sequence of SEQ ID NO: 115.170. The method of claim 169 , wherein the genetically modified yeast comprises one or more genetic modifications that increase a monooxygenase activity.171. The method of claim 170 , wherein the monooxygenase comprises CYP52A12 claim 170 , CYP52A13 claim 170 , CYP52A14 claim 170 , CYP52A15 claim 170 , CYP52A16 claim 170 , CYP52A17 claim 170 , CYP52A18 claim 170 , CYP52A19 or CYP52A20.172. The method of claim 169 , wherein the genetically modified yeast comprises one or more genetic modifications that increase a monooxygenase reductase activity.173. The method of claim 172 , wherein the monooxygenase reductase is cytochrome p450 reductase B (CPRB).174. The method of claim 169 , wherein the genetically modified yeast comprises one or more genetic modifications that disrupt activity of a POX4 polypeptide or a POX5 polypeptide.175Candida. The method of claim 169 , wherein the genetically modified yeast is a spp. yeast.176CandidaC. tropicalisC. viswanathii.. The method of claim 175 , wherein the spp. yeast is chosen from and177Candida. The method of claim 175 , wherein the spp. yeast is a genetically modified ATCC20336 yeast.178. The method of claim 169 , wherein the feedstock comprises a substantially pure oil.179. The method of claim 169 , wherein the ...

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Номер записи: 56
31-01-2019 дата публикации

Enhanced Production Of Fatty Acid Derivatives

Номер: US20190031594A1
Автор: HU Zhihao, Valle Fernando
Принадлежит: REG Life Sciences, LLC

Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels. 1. A recombinant host cell comprising:at least one gene exogenous gene encoding a fatty acid derivative enzyme selected from: a thioesterase, an acyl-CoA synthase, an alcohol forming-CoA reductase, an acyl-CoA reductase, an ester synthase and an alcohol dehydrogenase.2. The recombinant cell of claim 1 , wherein the at least one exogenous gene encoding the fatty acid derivative enzyme is over-expressed.3. The recombinant host cell of claim 1 , wherein the recombinant host cell comprises at least one exogenous thioesterase gene.4. The recombinant host cell of claim 3 , wherein the at least one thioesterase gene encodes a thioesterase having EC number: EC 3.1.2.- or EC 3.1.1.5 or EC 3.1.2.14.5. The recombinant host cell of claim 1 , wherein the recombinant host cell comprises at least one exogenous thioesterase gene claim 1 , an exogenous acyl-CoA synthase gene and at least one exogenous gene selected from the group consisting of an exogenous an acyl-CoA reductase gene claim 1 , an exogenous alcohol dehydrogenase gene and an exogenous fatty alcohol forming acyl-CoA reductase gene.6. The recombinant host cell of claim 5 , wherein the at least one thioesterase is: a thioesterase having EC number: EC 3.1.2.- or EC 3.1.1.5 or EC 3.1.2.14.7. The recombinant host cell of claim 6 , whereinthe least one exogenous gene selected from the group consisting of an exogenous an acyl-CoA reductase gene, an exogenous alcohol dehydrogenase gene and an exogenous fatty alcohol forming acyl-CoA reductase gene is: an exogenous an acyl-CoA reductase gene.8. A method for producing a fatty alcohol claim 6 , the method comprising:culturing, in the presence of a carbon source, a recombinant host cell, 'the recombinant host cell comprises: at least one gene exogenous ...

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Номер записи: 57
30-01-2020 дата публикации

STRUCTURING FATS AND METHODS OF PRODUCING STRUCTURING FATS

Номер: US20200032302A1
Автор: Bhat Riyaz, Bond Risha, Braksmayer Diza, Franklin Scott, Marangoni Alejandro, Rakitsky Walter, Rudenko George, Somanchi Aravind, ZHAO Xinhua
Принадлежит:

Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type. 198-. (canceled)99. A microbial oil comprising triglycerides , wherein the microbial oil has an altered fatty acid profile relative to a microbial oil produced by a non-recombinant oleaginous microbe , the microbial oil comprising at least 45% of triglycerides being a combination of palmitic-oleic-palmitic triglyceride , stearic-oleic-stearic triglyceride , and palmitic-oleic-stearic triglyceride , and wherein the oleaginous microbe comprises an exogenous gene encoding ketoacyl-ACP synthase (KAS).100. The microbial oil of claim 99 , wherein the ketoacyl-ACP synthase is selected from the group consisting of KASI claim 99 , KASII claim 99 , KASIII or KASIV.101. The microbial oil of claim 100 , wherein the ketoacyl-ACP synthase is KASII.102. The microbial oil of claim 101 , wherein the KASII has at least 95% sequence identity to a protein encoded by nucleotides 4039 to 5328 of SEQ ID NO: 95.103. The microbial oil of claim 99 , wherein the oleaginous microbe further comprises an exogenous gene that encodes a fatty acyl-ACP thioesterase claim 99 , lysophosphatidic acid acyltransferase (LPAAT) claim 99 , or sucrose invertase.104. The microbial oil of claim 99 , wherein the oleaginous microbe further comprises nucleic acids ...

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Номер записи: 58
04-02-2021 дата публикации

Recombinant yeast strain for producing nervonic acids and application thereof

Номер: US20210032665A1
Автор: Fuli Li, Huimin Meng, Jiaxin Li, Kai Zhang, Shian WANG, Weiming Fan
Принадлежит: Qingdao Institute of Bioenergy and Bioprocess Technology of CAS, ZHEJIANG ZHENYUAN PHARMACEUTICAL CO Ltd

The present invention discloses an engineering yeast strain for producing nervonic acids. The yeast strain over-expresses the genes related to enzymes required in a synthetic process of long-chain unsaturated fatty acids, such as fatty acid elongase, desaturase, diacylglycerol acyltransferase and the like, and optionally, further adjusts and controls the synthesis and decomposition route of triglyceride, the synthesis and decomposition route of sphingomyelin, and the synthesis and decomposition route and the oxidation-reduction balanced route of lipid subcell levels. The recombinant yeast strain can produce microorganism oil; and the content of the prepared nervonic acids accounts for 39.6% of the total fatty acids.

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Номер записи: 59
11-02-2016 дата публикации

Method to produce hydrocarbon from c-1 substrate

Номер: US20160040171A1
Автор: George Bennett, Ka-Yiu San, Mukund Karanjikar
Принадлежит: Technology Holding LLC, William Marsh Rice University

The disclosure relates to biological methods of making a hydrocarbon feedstock wherein one-carbon substrates are converted into useful chemicals and fuels. Particularly, genetically engineered bacteria are used to make C4-C10 fatty acids or derivatives from one-carbon substrates such as methanol and carbon dioxide.

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Номер записи: 60
07-02-2019 дата публикации

Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same

Номер: US20190040427A1
Автор: Dyson Lisa, Fyrst Henrik, Galgoczy David, Jansson Christer, Kurek Itzhak, Reed John S.
Принадлежит:

Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins. 1Cupriavidus, Xanthobacter, HydrogenobacterHydrogenovibrio. A bacterial cell of the genus , or comprising at least a first exogenous nucleic acid sequence , wherein the cell converts gaseous COand/or gaseous Hand/or syngas into one or more lipids or hydrocarbons.299.-. (canceled)100. The bacterial cell of claim 1 , wherein the first exogenous nucleic acid sequence encodes a fatty acyl-CoA binding protein.101. The bacterial cell of claim 100 , further comprising a second exogenous nucleic ...

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Номер записи: 61
07-02-2019 дата публикации

DECANEDIOIC ACID PRODUCED BY MICROBIAL FERMENTATION PROCESS AND PREPARATION METHOD THEREOF

Номер: US20190040428A1
Автор: Hao Yingli, Li Naiqiang, LIU Xiucai, XU Min, Yang Chen
Принадлежит:

The present invention provides a decanedioic acid produced by microbial fermentation process, in which the content of C10 aliphatic acid and C10 hydroxy aliphatic acid is maintained at a very low level. The present invention also provides a preparation method of the decanedioic acid and a polymer prepared by using the decanedioic acid as monomer. The decanedioic acid provided by the present invention is prepared by microbial fermentation process. The decanedioic acid product which is produced through the processes of microbial fermentation and separation has a higher purity, a higher thermal stability, and a lower impurity content. The decanedioic acid provided by the present invention could satisfy the requirements of high grade product of polyamide or polyester to produce polymer with excellent qualities. The preparation method of the decanedioic acid provided by the present invention which has many advantages of mild reaction conditions, environmental friendliness, high yield, and good product quality, may replace the chemical method to be used in industrial scale production of the decanedioic acid. 1. A decanedioic acid produced by microbial fermentation process , wherein the content of aliphatic acid comprising 10 carbons is less than 200 ppm , preferably less than 50 ppm , and the content of hydroxy aliphatic acid comprising 10 carbons is less than 300 ppm , preferably less than 100 ppm.2. The decanedioic acid produced by microbial fermentation process according to claim 1 , wherein the thermal stability of the decanedioic acid is more than 90% claim 1 , preferably more than or equal to 95% claim 1 , more preferably more than or equal to 97%.3. A preparation method of the decanedioic acid according to claim 1 , comprising:{'i': 'Candida tropicalis', 'culturing to prepare a seed;'}inoculating the obtained seed with fermentation medium;starting fermentation process to produce fermentation broth by adding decane; andpurifying the obtained fermentation broth to ...

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Номер записи: 62
15-02-2018 дата публикации

OMEGA-7 FATTY ACID COMPOSITION, METHODS OF CULTIVATION OF TRIBONEMA FOR PRODUCTION OF COMPOSITION AND APPLICATION OF COMPOSITION

Номер: US20180042839A1
Автор: Chen Lin, LIU Tianzhong, Wang Hui, Zhou Wenjun
Принадлежит: QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY, CHINESE ACADEMY OF SCIENCES

An omega-7 fatty acid composition from a raw material sp. obtained under heterotrophy and/or mixotrophy and a method of cultivation of sp. by way of heterotrophy and/or mixotrophy. The content of omega-7 fatty acid in the omega-7 fatty acid composition is 30% to 70%. The method of cultivation involves nutrient composition of a culture medium, cultivation conditions, and operation steps of a cultivation process. An application of the method in the production of biomass, lipid rich in omega-7 fatty acid, products with the biomass and/or the lipid as the raw material, etc. 1TribonemaTribonema. A method of cultivation of sp. , comprising the steps to carry out heterotrophy and/or mixotrophy of sp.2Tribonema. The method of cultivation of claim 1 , wherein the heterotrophy and/or the mixotrophy of the strain are/is implemented in a batch fermentation mode and/or a continuous fermentation mode and/or a fed-batch fermentation mode.3TribonemaTribonema aequale, Tribonema affine, Tribonema elegans, Tribonema gayanum, Tribonema intermixtum, Tribonema minus, Tribonema monochloron, Triconema angustissimum, Tribonema pyrenigerum, Tribonema regulare, Tribonema siderophilum, Tribonema spirotaenia, Tribonema ulotrichoides, Tribonema utriculosum, Tribonema viride, Tribonema vulgare, Tribonema yamadanum, Tribonema bombycinumTribonema vermichloris.. The method of cultivation of claim 1 , wherein the sp. is one or a combination of two or more selected from and4Tribonema. The method of cultivation of claim 1 , wherein the steps of the heterotrophy and/or the mixotrophy include adding a culture medium to a cultivation apparatus claim 1 , and inoculating the culture medium with a sterile strain for cultivation under a cultivation temperature of 2° C. to 40° C. claim 1 , a stirring rate of 1 rpm to 400 rpm claim 1 , an air bubbling rate of 0.01 vvm to 1 vvm claim 1 , a pH value of 5 to 10 claim 1 , and a light intensity of 0 μmol photons msto 1000 μmol photons ms.5. The method of cultivation ...

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Номер записи: 63
18-02-2021 дата публикации

CYANOBACTERIA HAVING IMPROVED PHOTOSYNTHETIC ACTIVITY

Номер: US20210047610A1
Автор: Carleton Michael, CARRIERI Damian, Hickman Jason W., Roberts James
Принадлежит:

This disclosure describes modified photosynthetic microorganisms, including Cyanobacteria, that have a reduced amount of a light harvesting protein (LHP) and contain one or more introduced or overexpressed polynucleotides encoding one or more enzymes associated with lipid biosynthesis, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides. 1111-. (canceled)112. A cell culture comprising a population of modified Cyanobacteria cells comprising genetic modifications including: wherein the genetic modification results in changes that, as compared to corresponding wild-type Cyanobacteria, include:', 'an increased level of NbIA polypeptide or a biologically active fragment of NbIA polypeptide that includes a conserved domain,', 'an enhanced level of photosynthetic activity, and', 'a reduced amount of a light harvesting protein (LHP); and, '(a) an addition of an endogenous NbIA gene, addition of an exogenous NbIA gene, or addition of a promoter that regulates NbIA gene expression,'}(b) an addition of a nucleic acid sequence comprising an additional exogenous gene encoding an exogenous polypeptide.1131. The cell culture of claim wherein the Cyanobacteria cell culture is cultured under light intensities between about(a) 200 micromol photons per square meter per second, and 'wherein the genetically modified Cyanobacteria cell culture expresses the exogenous polypeptide.', '(b) 2000 micromol photons per square meter per second, and'}114. The cell culture of wherein the exogenous polypeptide is a therapeutic polypeptide.115. The cell culture of wherein the exogenous polypeptide is a therapeutic polypeptide. This application claims priority to U.S. Provisional Patent Application No. 61/780,755 filed on Mar. 13, 2013, entitled “Cyanobacteria Having Improved Photosynthetic Activity,” which is hereby incorporated by reference in their entirety.The Sequence Listing associated with this application is provided in text format in lieu of ...

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Номер записи: 64
18-02-2016 дата публикации

Cyanobacteria Having Improved Photosynthetic Activity

Номер: US20160046902A1
Автор: Damian Carrieri, James Roberts, Jason W. Hickman, Michael Carleton
Принадлежит: Matrix Genetics LLC

This disclosure describes modified photosynthetic microorganisms, including Cyanobacteria, that have a reduced amount of a light harvesting protein (LHP) and contain one or more introduced or overexpressed polynucleotides encoding one or more enzymes associated with lipid biosynthesis, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides.

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Номер записи: 65
18-02-2016 дата публикации

Novel strain secreting fatty acids by phospholipase and method for producing fatty acids using it

Номер: US20160046968A1
Автор: KIM Eui Jin, LEE Jeong Kug, OH Eun Kyung, TONG Xiaomeng
Принадлежит:

A bacterial strain secreting fatty acids, the strain inducing fatty acids to be extracellularly secreted by using phospholipase expressed in the periplasmic space of cell. When a method of producing fatty acids by using the bacterial strain secreting fatty acids is used, fatty acids extracellularly secreted are continuously obtained without apoptosis, leading to lower costs and higher production efficiency. Phospholipase, unlike thioesterase, which is a typical fatty-acid degrading enzyme, decomposes phospholipid to produce free fatty acids. Accordingly, by using the substrate specificity of two different phospholipases, a fatty acid having a specific composition can be selectively produced. Unlike in a typical method in which fat is obtained from cells or tissues, fatty acids secreted during cell growth are obtainable by biding to a hydrophobic material without an extraction process using an organic solvent in large quantities. Accordingly, a more economical, environmentally friendly bio-oil production process can be realized. 1. A bacterial strain for secreting fatty acids toward the outside a cell by expressing phospholipase in the periplasmic space of cell.2. The strain of claim 1 , wherein the phospholipase is selected from the group consisting of phospholipase A1 and phospholipase A2.3Arabidopsis thaliana.. The strain of claim 2 , wherein the phospholipase A1 and the phospholipase A2 are derived from4. The strain of claim 1 , wherein a gene coding cytochrome c2 signal peptide is ligated to a gene coding the phospholipase so that the phospholipase is expressed in the periplasmic space of bacterial cell.5. The strain of claim 1 , wherein the strain further expresses at least one enzyme selected from the group consisting of acetyl-CoA carboxylase claim 1 , malonyl-CoA:ACP transacylase (FabD) claim 1 , and β-ketoacyl ACP synthase III (FabH) claim 1 , contributing to an increase in fatty-acid secretion efficiency.6. The strain of claim 1 , wherein the fatty acids ...

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Номер записи: 66
16-02-2017 дата публикации

HIGH YIELD ROUTE FOR THE PRODUCTION OF COMPOUNDS FROM RENEWABLE SOURCES

Номер: US20170044551A1
Автор: Chokhawala Harshal
Принадлежит:

Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as 1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a Caldehyde and pyruvate to a Cβ-hydroxyketone intermediate through an aldol addition; and b) converting the Cβ-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps. 1. A non-naturally occurring microbial organism comprising at least one exogenous nucleic acid encoding a 1 ,6-hexanediol pathway enzyme.2. The microbial organism of further comprising at least one enzyme selected from 2A wherein 2A is a 4-hydroxy-2-oxo-adipate aldolase claim 1 , or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate aldolase.327-. (canceled)28. A non-naturally occurring microbial organism claim 1 , comprising at least one exogenous nucleic acid encoding a 1 claim 1 ,6-hexanediol pathway enzyme selected from 2A and one or more of 2B claim 1 , 3B1 claim 1 , 3B2 claim 1 , wherein 2A is a 4-hydroxy-2-oxo-adipate aldolase or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate aldolase claim 1 , 2B is a 4-hydroxy-2-oxo-adipate dehydratase or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate 4-dehydratase claim 1 , 3B1 is a 4-hydroxy-2-oxo-adipate 2-reductase or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate 2-reductase claim 1 , and 3B2 is a 4-hydroxy-2-oxo-adipate 4-dehydrogenase or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate 4-dehydrogenase.29. The organism of claim 28 , further comprising a 1 claim 28 ,6-hexanediol pathway enzyme selected from one or more of 2C claim ...

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Номер записи: 67
16-02-2017 дата публикации

Micro-organism and Methods of Use

Номер: US20170044579A1
Автор: Finnegan Irene
Принадлежит:

The invention relates to a micro-organism comprising a hydrogenase enzyme system which is capable of converting carbon dioxide into formic acid and a second enzyme system which is capable of converting formic acid into aliphatic carboxylic acids having a chain length of five or more carbon atoms. Also described are various methods for producing oil, as well as other aspects of the invention. 1. A method of producing formic acid , comprising.{'i': 'Acetobacter', 'claim-text': {'br': None, 'sub': 2', '2, 'CO+H→HCOOH.'}, 'contacting a culture medium comprising a micro-organism consisting of the strain having accession number NCIMB 41808, or a micro-organism derived therefrom, with carbon dioxide under conditions permitting reduction of the carbon dioxide to formic acid2. The method of claim 1 , wherein the micro-organism is present in the culture medium at 20%.3. The method of claim 1 , further comprisingintroducing a disinfectant or antimicrobial agent into the culture medium to kill the micro-organism.4. The method of claim 3 , wherein the micro-organism is killed prior to contacting the culture medium with CO2.5. The method of claim 1 , wherein the culture medium comprises a minimal medium.6. The method of claim 1 , wherein carbon dioxide is the sole source of carbon.7. The method of claim 6 , wherein carbon dioxide is bubbled through the medium.8. The method of claim 1 , wherein the conditions comprise a pH of between about 3.0 and about 8.5.9. The method of wherein the conditions comprise a pH between about 6.0 and about 7.0.10. The method of claim 1 , wherein the conditions comprise a temperature of between about 5° C. and about 60° C.11. The method of claim 10 , wherein the conditions comprise a temperature of between about 15° C. and about 20° C.12. The method of claim 1 , wherein the medium comprises a carbon dioxide sequestering agent.13. The method of claim 1 , wherein the medium comprises an oxidising agent.14. The method of claim 1 , wherein the culture ...

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Номер записи: 68
16-02-2017 дата публикации

Method of Producing Lipid by Using Beta-Ketoacyl-ACP Synthase

Номер: US20170044580A1
Автор: Endo Hiroko, HORI Koichi, OHTA Hiroyuki, OZAKI Tatsuro, Saito Takeshi, Sugihara Shinji, Tojo Takuto
Принадлежит: KAO CORPORATION

A method of producing a lipid, containing the following steps (1) and (2); and a transformant obtained by introducing a gene encoding the following protein (a) or (b) into a host: 2. The method of producing a lipid according to claim 1 , wherein the lipid is a medium chain fatty acid or an ester thereof.3. The method of producing a lipid according to claim 1 , wherein the protein (a) or (b) is a β-ketoacyl-ACP synthase having the specificity to a medium chain acyl-ACP.4. The method of producing a lipid according to wherein the host is a microorganism or a plant5. The method of producing a lipid according to claim 1 , wherein the step (2) comprises culturing the transformant in a cerulenin-containing medium.6. The method of producing a lipid according to claim 4 , wherein the host is a microorganism.7. The method of producing a lipid according to claim 6 , wherein the microorganism is a microalga.8Escherichia coli.. The method of producing a lipid according to claim 6 , wherein the microorganism is10. The transformant according to claim 9 , wherein the host is a microorganism or a plant.11. The transformant according to claim 10 , wherein the host is a microorganism.12. The transformant according to claim 11 , wherein the microorganism is a microalga.13Escherichia coli.. The transformant according to claim 11 , wherein the microorganism is15. A gene encoding the protein according to . The present invention relates to B-ketoacyl-ACP synthase, and a method of producing a lipid by using the same.Fatty acids are one of the principal components of lipids. In vivo, fatty acids are bonded to glycerin via an ester bond to form lipids such as triacyiglycerol Many animals and plants store and utilize fatty acids as an energy source. These fatty acids and lipids (fats and oils) stored in animals and plants are widely utilized for food or industrial use.For example, higher alcohol derivatives that are obtained by reducing higher fatty acids having approximately 12 to 18 carbon ...

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Номер записи: 69
19-02-2015 дата публикации

Treatment of biomass

Номер: US20150050707A1
Автор: Anderson AGGREY, Daniel James Gapes, Peter James STRONG, Robert Jason LEI, Trevor Raymond Stuthridge
Принадлежит: Terax LP

A process for the treatment of biomass comprising subjecting biomass to microbial digestion to produce volatile fatty acids and/or solvents followed by wet oxidation to reduce biosolid volume while retaining or increasing the concentration of the volatile fatty acids and/or solvents.

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Номер записи: 70
25-02-2021 дата публикации

HUMAN AND NON-HUMAN ANIMAL USE OF MICROBIAL ANAPLEROTIC OIL

Номер: US20210051988A1
Автор: Amezquita Ernult Magdalena, Ganuza Taberna Eneko, LaMont Micheal
Принадлежит:

Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Further, disclosed are examples of incorporation of these higher concentration OCFA products into food for human and non-human animal consumption. 1. A food additive comprising microalgal anaplerotic oil rich in saturated tridecanoic (C13:0) , pentadecanoic (C15:0) , and heptadecanoic (C17:0) odd-chain fatty acids (OCFA) , the microalgal anaplerotic oil derived from a thraustochytrid strain cultured aerobically with added propionate and a continuous supply of oxygen resulting in elevated levels of saturated tridecanoic (C13:0) , pentadecanoic (C15:0) , and heptadecanoic (C17:0) OCFA in a microalgal mass of the thraustochytrid strain , wherein:at least five percent of the microalgal anaplerotic oil from the thraustochytrid strain's total fatty acid (TFA) content is saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA; andat least one percent cell dry weight (CDW) of the microalgal mass from the thraustochytrid strain is saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA.2. The food additive of claim 1 , wherein pentadecanoic acid makes up greater than eighteen percent of the TFA content of the microalgal anaplerotic oil.3. The food additive of claim 1 , wherein heptadecanoic acid makes up greater than five percent of the TFA content of the microalgal anaplerotic oil.4. The food additive of claim 1 , wherein saturated tridecanoic (C13:0) claim 1 , pentadecanoic (C15:0) claim 1 , and heptadecanoic (C17:0) OCFA comprise a range of 5% to 60% of the TFA content of the microalgal anaplerotic oil.5. The food additive of claim 1 , wherein the microalgal mass is processed for human and non-human animal consumption claim 1 , and ...

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Номер записи: 71
08-05-2014 дата публикации

Microorganisms and methods for production of specific length fatty alcohols and related compounds

Номер: US20140127765A1
Автор: Anthony P. Burgard, Robin E. Osterhout
Принадлежит: Genomatica Inc

The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid.

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Номер записи: 72
14-02-2019 дата публикации

METHODS OF PRODUCING LIPIDS

Номер: US20190048381A1
Автор: Heller David, McNamara Harold M., Shumaker Andrew, Ticku Shara
Принадлежит:

Described herein are microoganisms and methods for producing lipids by co-culturing a photosynthetic microorganism with a heterotrophic microorganism to produce a culture medium having a titer of lipids. 1. A method of producing lipids , the method comprising co-culturing a photosynthetic microorganism with a heterotrophic microorganism to produce a culture medium having a titer of lipids of at least 300 mg/L.2. The method of claim 1 , wherein the photosynthetic microorganism comprises a cyanobacterium or alga.3. (canceled)4Chlorella.. The method of claim 2 , wherein the cyanobacterium is selected from genera Synechococcus and5. The method of claim 1 , wherein the photosynthetic microorganism expresses sugar transport proteins.6. The method of claim 1 , wherein the heterotrophic microorganism comprises a yeast claim 1 , or an oleaginous yeast.7. (canceled)8YarrowiaCandidaYarrowia lipolytica.. The method of claim 6 , wherein the yeast is selected from genera Rhodosporidium claim 6 , claim 6 , and claim 6 , or from Rhodosporidium toruloides and9. (canceled)10. A method of producing lipids claim 6 , comprising co-culturing a photosynthetic microorganism with a heterotrophic microorganism claim 6 , wherein at least 30% of a continuous culture dry cell weight is the heterotrophic microorganism.11. The method of claim 10 , wherein the photosynthetic microorganism comprises a cyanobacterium or alga.12. (canceled)13Chlorella.. The method of claim 10 , wherein the cyanobacterium is selected from genera Synechococcus and14. The method of claim 1 , wherein the photosynthetic microorganism expresses sugar transport proteins.15. The method of claim 10 , wherein the heterotrophic microorganism comprises a yeast claim 10 , or oleaginous yeast.16. (canceled)17YarrowiaCandida.. The method of claim 15 , wherein the yeast is selected from genera Rhodosporidium claim 15 , claim 15 , Metschnikowia claim 15 , and18Yarrowia lipolyticaMetschnikowia pulcherrima. The method of claim 15 , ...

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Номер записи: 73
22-02-2018 дата публикации

Concentrated Solution of 17-Hydroxydocosahexaenoic Acid

Номер: US20180050005A1
Автор: DiMauro Thomas M, Wildenhaus Kevin
Принадлежит: Janssen Pharmaceutica NV

A method of treating a mother having postpartum depression, comprising administering to the mother a composition comprising a plurality of mixed self-assemblies comprising: 129-. (canceled)30. A method of treating a mother having postpartum depression , comprising administering to the mother a composition comprising a plurality of mixed self-assemblies comprising:i) at least 50 wt % of a soyasaponin, andii) allopregnanolone. This application claims priority from co-pending provisional applications U.S. Ser. No. 62/375,676, filed Aug. 16, 2016 (DiMauro et al.), entitled “Self-Assemblies Comprising Intercalated Natural GABA(A) Delta Agonists” (Docket No. PRD3425USPSP) and U.S. Ser. No. 62/402,439, filed Sep. 30, 2016 (DiMauro et al.), entitled “Tailored Novel Nutriceutical Solutions to the Heterogeneous Phenotypes of Perinatal Depression” (Docket No. PRD3425USPSP1), the specifications of which are incorporated by reference in their entireties.The loving connection between a mother and her baby is a special bonding that can benefit the baby not only in the present, but also well into the future. Bonding brings the mother and child closer together, and this positive attachment can enhance the baby's wellbeing and later development. Because a healthy bond between the mother and her newborn infant is crucial to the proper development of the child, loving efforts to strengthen that bond are highly valued. Some of the ways in which a healthy mother can show love for her child and promote this bonding is by experiencing joy at her child's smile and by providing appropriate attention to her child's needs.It has been estimated that over 700,000 mothers are afflicted with postpartum depression (PPD) each year in the United States. PPD is considered to be a major depression, and is characterized by standard depressive features. Typical PPD symptoms include non-responsiveness towards the infant's needs and an absence of joy that is normally associated with healthy parent-child ...

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Номер записи: 74
25-02-2016 дата публикации

Engineered Organisms for Production of Novel Lipids

Номер: US20160053271A1
Автор: ÁLVAREZ Héctor, ARABOLAZA Ana, COMBA Santiago, GRAMAJO Hugo, MENENDEZ- BRAVO Simón
Принадлежит:

The present disclosure provides engineered microorganisms, engineered biosynthetic pathways, methods of producing lipid compounds using genetically engineered microorganisms, and the products synthesized by those organisms. In particular, the disclosure provides genetically engineered microorganisms for the production of multi-methyl branched fatty acids (MMBFAs) and MMBFA esters (wax esters) derived from these fatty acids. In addition, the disclosure provides methods for producing acylglycerols with one of more of their acyl substituents being an MMBFA, and methods for producing alcohols derived from MMBFAs (fatty alcohols). 1101-. (canceled)102. An engineered microorganism comprising:(i) an exogenous or overexpressed polyketide synthase or active fragment thereof, and(ii) an exogenous or overexpressed long chain fatty acid AMP ligase,wherein said recombinant microorganism comprises a multi-methyl branched fatty acid (MMBFA) synthesis pathway in which the exogenous or overexpressed polyketide synthase MAS catalyzes the iterative conversion of methyl-malonyl-CoA to MMBFA.103. The engineered microorganism according to claim 102 , wherein the polyketide synthase is a MAS (mycocerosic acid synthase) or active fragment thereof comprising an acyl transferase (AT) domain and an acyl carrier protein (ACP) domain and a ketide synthase (KS) domain.104. The engineered microorganism according to claim 102 , wherein the polyketide synthase or active fragment thereof comprises SEQ ID NO: 1 claim 102 , or a homolog claim 102 , fragment claim 102 , or variant thereof.105Mycobacterium tuberculosis.. The engineered microorganism according to claim 102 , wherein the polyketide synthase or active fragment thereof comprises an amino acid sequence having at least 80% sequence identity to PKS2 (SEQ ID NO:15) claim 102 , PKS3 (SEQ ID NO:30) claim 102 , PKS4 (SEQ ID NO:26) claim 102 , PKS5 (SEQ ID NO:37) claim 102 , PKS7 (SEQ ID NO:48) claim 102 , or PKS12 (SEQ ID NO:61) from106. The ...

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Номер записи: 75
25-02-2021 дата публикации

ANAPLEROTIC OIL PRODUCTION IN MICROBIALS

Номер: US20210054421A1
Автор: Amezquita Ernult Magdalena, Ganuza Taberna Eneko, Sellers Charles
Принадлежит:

Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Such compositions can comprise pentadecanoic and heptadecanoic fatty acids. The techniques described herein include methods for producing and deriving such compositions rich in odd-chain fatty acids from microbials, including microalgae and yeasts/fungi. 1. A method for producing anaplerotic oil from microalgae , the method comprising:adding the microalgae to a culture medium, wherein the microalgae are from the family of thraustochytrids;adding propionate to the culture medium resulting in cultured microalgae with enhanced synthesis of saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) odd-chain fatty acid (OFCA) and increased total amounts of tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) present in the microalgae; andproducing an anaplerotic oil from the cultured microalgae, wherein at least five percent of the total fatty acids (TFA) of the anaplerotic oil are saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA and the saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) make up at least one percent of the cell dry weight (CDW) of the microbial mass.2. The method of claim 1 , wherein adding propionate comprises adding the propionate to produce OCFAs in a range of 5 and 70% TFAs.3. The method of claim 1 , wherein the propionate is added with a carbon source at a weight to weight ratio below 0.1 of propionate to carbon source.4. The method of claim 1 , wherein adding propionate comprises adding the propionate in a batch system into the culture medium.5. The method of claim 1 , wherein adding propionate comprises adding the propionate in a fed-batch system into the ...

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Номер записи: 76
13-02-2020 дата публикации

MICROORGANISMS AND METHODS FOR THE PRODUCTION OF FATTY ACIDS AND FATTY ACID DERIVED PRODUCTS

Номер: US20200048666A1
Автор: COPLEY Shelley, Evans Ron, HOGSETI Dave, Liao Hans, LIPSCOMB Matthew, Lipscomb Tanya, Louie Michael, Lynch Michael, Prather Brittany, Spindler Eileen
Принадлежит: Cargill, Incorporated

This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism. 142-. (canceled)43. A genetically modified organism comprising:a heterologous nucleic acid encoding a first NphT7 polypeptide; anda heterologous nucleic acid encoding a second NphT7 polypeptide, the second NphT7 polypeptide comprising one or more amino acid substitutions at a position selected from the group consisting of Ser84, Thr85, Gln90, Val114, Tyr144, Ile147, Val157, Phe159, Ile194, Val196, Phe217, Gly288, and Gly318,wherein said microorganism produces a fatty acid or fatty acid-derived product having a carbon chain length of C6 or greater.44. The genetically modified organism of wherein the second NphT7 polypeptide comprises amino acid substitutions at Ile147 and Phe217.45. The genetically modified organism of wherein the second NphT7 polypeptide comprises amino acid substitutions of I147S and F217V.46. The genetically modified organism of claim 43 , wherein the fatty acid or fatty acid-derived product has a carbon chain length of C8 or greater.47. The genetically modified organism of claim 43 , wherein the fatty acid or fatty acid-derived product has a carbon chain length of C10 or greater.48. The genetically modified organism of claim 43 , wherein the microorganism further comprises:a heterologous nucleic acid encoding a ketoacyl-CoA reductase;a heterologous nucleic acid encoding a hydroxyacyl-CoA dehydratase; anda heterologous nucleic acid encoding an enoyl-CoA reductase.49. The genetically modified organism of claim 48 , wherein:the ketoacyl-CoA reductase is selected from the group consisting of SEQ ID NO: 183 and SEQ ID NO: 137;the hydroxyacyl-CoA dehydratase is selected from the group consisting of ...

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Номер записи: 77
22-02-2018 дата публикации

MATERIALS AND METHODS FOR CHARACTERIZING AND USING A 3-KETOACYL-ACYL CARRIER PROTEIN (ACP) SYNTHASE III (KASIII) FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS

Номер: US20180051263A1
Автор: GARG Shivani, JIN Huanan, Nikolau Basil J., Yandeau-Nelson Marna
Принадлежит:

A mutant , which does not express a functional KASIIIA and/or KASIIIB, and method of making; a mutant , which does not express a functional PhaC1, PhaC2, and/or PhaC3, and method of making; method of characterizing substrate specificity of KASIII; method of making mutant KASIII with altered substrate specificity and/or altered level of activity and nucleic acid, vector, host cell/organism, and mutant KASIII; an in vitro, high-throughput spectrophotometric method of assaying KASIII activity; and materials and methods for using KASIII for production of bi-functional fatty acids and the materials so produced. 1Bacillus subtilis. A mutant , which does not express a functional 3-ketoacyl-acyl carrier protein (ACP) synthase II (KASIII) A and does not express a functional KASIIIB , and wherein the KASIIIB of SEQ ID NO: 66 comprises a mutation of Trp221 and a mutation of Val226.2Bacillus subtilis. The mutant of claim 1 , wherein the mutation of Trp221 is Trp221 Val and the mutation of Val226 is Val226Leu.3Bacillus subtilis. The mutant of claim 1 , wherein the KASIIIA of SEQ ID NO: 64 comprises a mutation of Met213 claim 1 , alone or in further combination with Phe208.4Bacillus subtilis. The mutant of claim 3 , wherein the mutation of Met213 is Met213Leu and the mutation of Phe208 is Phe208Val.5B. subtilisB. subtilis. A method of making the mutant of claim 1 , which method comprises introducing into wild-type a mutation that prevents expression of a functional KASIIIA and a mutation that prevents expression of a functional KASIIIB claim 1 , wherein Trp221 and Val226 are mutated in the KASIIIB of SEQ ID NO: 66.6. The method of claim 5 , wherein Trp221 is mutated to Val and Val226 is mutated to Leu.7. The method of claim 5 , wherein Met213 claim 5 , alone or in further combination with Phe208 claim 5 , is mutated in the KASIIIA of SEQ ID NO: 64.8. The method of claim 7 , wherein Met213 is mutated to Leu and Phe208 is mutated to Val. This application is a Divisional of U.S. ...

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Номер записи: 78
26-02-2015 дата публикации

METHOD FOR PRODUCING MEDIUM-CHAIN OMEGA-HYDROXY FATTY ACIDS, ALPHA,OMEGA-DICARBOXYLIC ACIDS, AND OMEGA-AMINO FATTY ACIDS FROM LONG-CHAIN FATTY ACIDS BY BIOTRANSFORMATION

Номер: US20150057461A1
Автор: Jeon Eun Yeong, Park Jin Byung, Song Ji Won
Принадлежит:

The present invention relates to a transformant which is transformed to express Baeyer-Villiger monooxygenase (BVMO), a method for producing C5-C14 medium-chain ω-hydroxy fatty acids, α,ω-dicarboxylic acids, ω-amino fatty acids, or alcohols from C16-C20 long-chain fatty acids by biotransformation using the transformant, a method for producing a fatty acid derivative having an ester group which is introduced into the chain thereof from keto fatty acid using the BVMO, and novel ω-hydroxy fatty acids which are prepared by the method. Degradation products such as C5 to C14 ω-hydroxy fatty acids, α,ω-dicarboxylic acids, ω-amino fatty acids, alcohols can be produced in a large amount from C16 to C20 long-chain fatty acids contained in a medium by biotransformation using a transformant capable of expressing BVMO of the present invention. Therefore, it can be widely used to produce ω-hydroxy fatty acids, α,ω-dicarboxylic acids, ω-amino fatty acids or alcohols in a more safe and economic manner. 1. A transformant introduced with Baeyer-Villiger monooxygenase (BVMO)-encoding gene.2Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas veronii, Rhodococcus jostiiPseudomonas. The transformant according to claim 1 , wherein the BVMO is derived from or sp. strain HI-70.3. The transformant according to claim 1 , wherein a hydratase- or lipoxygenase-encoding gene claim 1 , an alcohol dehydrogenase-encoding gene claim 1 , an ester hydrolase-encoding gene claim 1 , an aminotransferase-encoding gene or a combination thereof is further introduced.4. The transformant according to claim 3 , wherein the hydratase-encoding gene claim 3 , the alcohol dehydrogenase-encoding gene claim 3 , the BVMO-encoding gene and the ester hydrolase-encoding gene are introduced to produce medium-chain ω-hydroxy fatty acids claim 3 , or α claim 3 ,ω-dicarboxylic acids and alcohols from long-chain fatty acids.5. The transformant according to claim 4 , wherein ω-hydroxynonanoic acid (C9) is produced as the ...

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Номер записи: 79
03-03-2016 дата публикации

PROCESS FOR ENHANCING THE VIABLE COUNTS OF LACTIC ACID BACTERIA AND USEFUL COMPOSITIONS THEREOF

Номер: US20160058805A1
Автор: Ali Furqan, Arumugam Sivakumar, Majeed Muhammed, Nagabhushanam Kalyanam
Принадлежит:

The present invention disclosed herein describes (i) the growth promotional activity of natural plant based fibres on MTCC 5856; (ii) the combination of natural plant based fibres and MTCC 5856 to inhibit Gram Negative pathogenic bacteria and (iii) the production of short chain fatty acids (SCFA) by MTCC 5856 using plant based natural fibres. 1Bacillus coagulatesBacillus coagulansTrigonella foenumgraecumLycium barbarumLinum usitatissimumCocos nuciferaZingiber officinaleEmblica officinalisPlantago ovataVaccinium oxycoccos. A method of increasing the viable colony count of MTCC 5856 said method comprising step of growing MTCC 5856 in the presence of natural plant fibres selected from the group consisting of -(fenugreek) seed fibers , seed fibers , (Flax) seed fibers , (Coconut) fibers , (Ginger) rhizome fibers , (Amla) fruit fibers , (Psyllium) fibers and (Cranberry) seed fibers.2Bacillus coagulansTrigonella foenumgraecumLycium barbarumLinum usitatissimumCocos nuciferaZingiber officinaleEmblica officinalisPlantago ovataVaccinium oxycoccos. A method of inhibiting pathogenic Gram Negative bacteria said method comprising step of bringing to contact said Gram Negative bacteria with MTCC 5856 co-cultured with natural plant fibres selected from the group consisting of -(fenugreek) seed fibers , seed fibers , (Flax) seed fibers , (Coconut) fibers , (Ginger) rhizome fibers , (Amla) fruit fibers , (Psyllium) fibers and (Cranberry) seed fibers.3Bacillus coagulansTrigonella foenumgraecumLycium barbarumLinum usitatissimumCocos nuciferaZingiber officinaleEmblica officinalisPlantago ovataVaccinium oxycoccos. A method of producing short chain fatty acids by co-culturing MTCC 5856 with natural plant fibres selected from the group consisting of -(fenugreek) seed fibers , seed fibers , (Flax) seed fibers , (Coconut) fibers , (Ginger) rhizome fibers , (Amla) fruit fibers , (Psyllium) fibers and (Cranberry) seed fibers.4Bacillus coagulansTrigonella foenumgraecumLycium barbarumLinum ...

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Номер записи: 80
10-03-2022 дата публикации

ARTIFICIAL RIBOSOMES FOR FULLY PROGRAMMABLE SYNTHESIS OF NONRIBOSOMAL PEPTIDES

Номер: US20220073897A1
Автор: Green Alexander A.
Принадлежит:

Provided herein, in some embodiments, are artificial ribosomes that synthesize nonribosomal peptides, polyketides, and fatty acids with full control over peptide sequence. Also provided herein are methods for programmed synthesis of nonribosomal peptides, polyketides, and fatty acids. In particular, provided herein are methods for scalable synthesis of a wide range of antibacterial, antifungal, antiviral, and anticancer compounds. 114.-. (canceled)16. The molecular assembly line of claim 15 , wherein the synthetic RNA scaffold is a tRNA analog.17. The molecular assembly line of claim 15 , wherein at least one fusion protein comprises a RNA-binding peptide selected from Lambda(G1N2R4) claim 15 , P22N claim 15 , RevN7D claim 15 , HTLV-1-Rex claim 15 , and BIV-Tat.18. The molecular assembly line of claim 17 , wherein at least one RNA binding peptide binds to an aptamer sequence within a tRNA analog.19. A synthetic nucleic acid sequence encoding the molecular assembly line of .20. A biological cell comprising the synthetic nucleic acid sequence of .21. A method for assembling the molecular assembly line of claim 15 , the method comprising:(a) providing the molecular assembly line to a cell-free expression system; and(b) incubating the cell-free system containing the molecular assembly line under conditions wherein the at least two fusion proteins are able to bind to the synthetic RNA scaffold.22. The method of claim 21 , wherein the synthetic RNA scaffold comprises tRNA analogs.23. The method of claim 21 , wherein at least one RNA-binding peptide comprises an RNA-binding domain from a protein selected from Lambda N claim 21 , P22N claim 21 , RevN7D claim 21 , HTLV-1-Rex claim 21 , and BIV-Tat.24. The method of claim 23 , wherein at least one RNA-binding peptide binds to an aptamer sequence within a tRNA analog. This application claims priority to U.S. Provisional Application No. 62/616,061, filed Jan. 11, 2018, which is incorporated herein by reference as it set forth ...

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Номер записи: 81
04-03-2021 дата публикации

Stearoyl-ACP Desaturase and Variants Thereof Capable of Dioxygenase Chemistry and Converting Oleoyl-ACP to erythro-9,10-Dihydroxystearate

Номер: US20210062163A1
Автор: Shanklin John, Whittie Edward J.
Принадлежит:

The invention provides wild type stearoyl-ACP type desaturase, and its mutants, particularly T117R and D280K, for converting oleoyl-ACP, the normal product of the stearoyl-ACP desaturase, to a vicinal diol, erythro 9, 10 dihydroxy stearate. The invention provides mutant or variant stearoyl-ACP type desaturase polypeptides having one or more amino acid substitutions, particularly one or more substitution at amino acid 117 and/or amino acid 280, of the plastid enzyme polypeptide. The mutant polypeptides provide for higher vicinal diol, particularly 9, 10 dihydroxy stearate, compared to wild type stearoyl-acyl carrier protein (ACP) desaturase, including when the mutant stearoyl-ACP type desaturase is expressed in host cells. Also provided are polynucleotides encoding the mutant stearoyl-ACP type desaturase, constructs and host cells comprising the polynucleotides, methods for producing a vicinal diol, erythro 9, 10 dihydroxy stearate, in host cells. The invention also relates to plants, particularly transgenic or recombinantly engineered plants, expressing one or more of the mutant a vicinal diol, erythro 9, 10 dihydroxy stearate polypeptides, as well as seeds derived from the plants. 1. A mutant plant diiron enzyme polypeptide capable of a dioxygenase reaction mechanism wherein a double bond is converted to a vicinal diol.2. The mutant of which is a mutant stearoyl-acyl carrier protein (ACP) desaturase polypeptide capable of catalyzing the conversion of oleoyl-ACP to erythro 9 claim 1 ,10 dihydroxy stearate comprising:(a) an amino acid replacement of the threonine (T) at amino acid residue 117 of the processed plastid polypeptide sequence and corresponding to residue 117 of SEQ ID NO: 2 or of the amino acid at the corresponding position in a plant stearoyl-ACP desaturase polypeptide;(b) an amino acid replacement of the aspartic acid (D) at amino acid residue 280 of the processed plastid polypeptide sequence and corresponding to residue 280 of SEQ ID NO: 2 or of the ...

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Номер записи: 82
22-05-2014 дата публикации

Low Polysaccharide Microorganisms for Production of Biofuels and Other Renewable Materials

Номер: US20140137463A1
Автор: Apt Kirk, Behrens Paul W., Borden Jacob, Dain David, HANSEN Jon, Pfeifer Joseph W.
Принадлежит: BP CORPORATION NORTH AMERICA INC.

High cell density fermentations of wild-type organisms can result in increased viscosity due to the production of exocellular polysaccharides. Mutant microorganisms with a dry morphology, resulting from reduced exocellular polysaccharide formation, were isolated and characterized. The exocellular polysaccharide composition for these modified microorganisms is shown to be different than the polysaccharide composition of the wild type microorganism. In addition to reduced exocellular polysaccharide formation, dry morphology mutants of multiple strains show reduced viscosity, improved oxygen mass transfer, and improved fatty acid fermentation yield on carbon. 1. An oleaginous microorganism suitable for production of renewable materials , wherein the microorganism comprises a genetic modification not present in an unmodified microorganism , and wherein the modified microorganism produces a fermentation broth having a lower viscosity than a fermentation broth produced by the unmodified microorganism when grown in culture.2. The oleaginous microorganism of claim 1 , wherein the modified microorganism produces a fermentation broth comprising a biomass of at least about 50 grams cellular dry weight per liter and a viscosity of less than about 1 claim 1 ,100 centipoise (cP).3. The oleaginous microorganism of claim 1 , wherein the modified microorganism produces a fermentation broth comprising a biomass of at least about 50 grams cellular dry weight per liter and a viscosity of less than about 30 cP.4. The oleaginous microorganism of claim 1 , wherein the modified microorganism comprises a dry morphology claim 1 , while the unmodified microorganism does not comprise a dry morphology.5. The oleaginous microorganism of claim 1 , the microorganism being the microorganism corresponding to one or more of ATCC Deposit No. PTA-12508 (Strain MK29404 (Dry1-13J)) claim 1 , ATCC Deposit No. PTA-12509 (Strain MK29404 (Dry1-182J)) claim 1 , ATCC Deposit No. PTA-12510 (Strain MK29404 (Dry1 ...

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Номер записи: 83
05-03-2015 дата публикации

PRODUCTION OF FATTY ACID DERIVATIVES

Номер: US20150064782A1
Автор: Clarke Elizabeth J., Da Costa Bernardo M., Greenfield Derek L., Groban Eli S., HU Zhihao, Schirmer Andreas W.
Принадлежит: REG Life Sciences, LLC

The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell.

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Номер записи: 84
10-03-2016 дата публикации

METHOD FOR PRODUCING HYDROCARBIDES

Номер: US20160068866A1
Автор: DUPRE Jean-Yves, RODRIGUEZ Oswaldo Yaguez
Принадлежит: BIO-THINK

A method for producing hydrocarbons, includes at least the following steps: a) anaerobic fermentation of a fermentable raw material in order to produce volatile fatty acids, b) elongation of the volatile fatty acids produced in step a) by fermentation with at least one bacterium of the genus, extraction of the fatty acids produced from the fermentation broth, and c) production of hydrocarbons by subjecting the fatty acids produced in step b) to a Kolbe electrolysis. 1. A process for producing hydrocarbons , comprising at least the implementation of the following stages:a) conducting anaerobic fermentation of a fermentable raw material for obtaining volatile fatty acids,{'i': 'Megasphaera,', 'b) elongating the volatile fatty acids obtained in stage a) by carrying out fermentation of the volatile fatty acids with one or more bacteria of the genus obtaining a fermentation broth, and extracting fatty acids obtained from the fermentation broth, and'}c) obtaining hydrocarbons by subjecting the fatty acids obtained in stage b) to Kolbe electrolysis.2Megasphaera elsdenii.. The process for producing hydrocarbons according to claim 1 , wherein stage b) is carried out by fermentation with the bacterium3. The process for producing hydrocarbons according to claim 1 , wherein stage b) is carried out with a pH between 5.5 and 6.5.4. The process for producing hydrocarbons according to claim 1 , wherein stage b) is carried out with an redox potential between −500 and −600 mV.5Megasphaera. The process for producing hydrocarbons according to claim 1 , wherein stage b) for fermentation of volatile fatty acids with one or more bacteria of the genus is carried out under conditions selected from the group consisting of under a cover of methane or with bubbling of methane claim 1 , under a cover of biogas or with bubbling of biogas claim 1 , under a cover of a mixture of methane claim 1 , carbon dioxide claim 1 , and hydrogen or with bubbling of a mixture of methane claim 1 , carbon ...

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Номер записи: 85
08-03-2018 дата публикации

MICROBIOLOGICAL PRODUCTION OF SHORT FATTY ACIDS AND USES THEREOF

Номер: US20180066240A1
Автор: BOLES Eckhard, Gajewski Jan, Grininger Martin, Pavlovic Renata
Принадлежит:

The present invention relates to proteins involved in fatty acid synthesis, such as fatty acid synthases (FAS) variants, comprising one or more polypeptide chains, wherein said polypeptide chain(s) comprise one or more subunits comprising a malonyl/palmitoyl transferase domain (MPT domain), acetyl transferase domain (AT domain), and ketoacyl synthase domain (KS domain), and at least one amino acid substitution in the MPT domain at a position corresponding to R130, in the AT domain at a position corresponding to I306, and/or in the KS domain, preferably in the acyl binding channel and/or at KS domain binding site to ACP, to modulate affinities of acyl intermediates, and optionally further amino acid substitution(s). The present invention relates to the respective polypeptide domains. The present invention further relates to nucleic acid molecules encoding the proteins (or the polypeptide domains) and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of short fatty acids, CoA esters of short fatty acids, ethyl esters of short fatty acids, esters of short fatty acids with other metabolites, and/or enzyme bound short fatty acids (Cto C), comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention further relates to a method for the production of biofuels, flavoring compounds and/or fine chemicals, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the proteins, nucleic acids molecule or host cells for the bulk production of short fatty acids (Cto C), the specific production of Cfatty acids and/or C8 fatty acids, the bulk production of CoA esters of short fatty acids (Cto C), the specific production of C-CoA esters and/or C-CoA esters, the bulk production of ethyl esters of short fatty acids (Cto C), the specific production of Cfatty acid ethyl esters and/or ...

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Номер записи: 86
27-02-2020 дата публикации

OIL COMPOSITION AND METHOD OF RECOVERING SAME

Номер: US20200063168A1
Автор: BOOTSMA Jason
Принадлежит: POET RESEARCH, INC.

The present invention generally relates to oil compositions and methods of producing such oil compositions. More particularly, the present invention relates to an oil composition recovered from a fermentation product as well as methods of recovering such oil compositions for use in various processes such as bio-diesel production as well as in various edible applications. 1126-. (canceled)127. A composition comprising corn oil , wherein the corn oil comprises:no greater than 15% w/w of a free fatty acid based on the total weight of the composition;an iodine value of no greater than 125;no greater than 1% w/w of moisture and insolubles combined based on the total weight of the composition; and at least 50 mcg/g of lutein,', 'at least 10 mcg/g of cis-lutein/zeaxanthin,', 'at least 5 mcg/g of alpha-cryptoxanthin,', 'at least 5 mcg/g of beta-cryptoxanthin,', 'at least 0.5 mcg/g of alpha-carotene,', 'at least 0.1 mcg/g of cis-beta-carotene, and', 'a combination of two or more thereof., 'a component selected from the group consisting of128. The composition of claim 127 , wherein the composition comprises at least 75 mcg/g of the lutein.129. The composition of claim 127 , wherein the composition comprises at least 30 mcg/g of the cis-lutein/zeaxanthin.130. The composition of claim 127 , wherein the composition comprises at least 10 mcg/g of the alpha-cryptoxanthin.131. The composition of claim 127 , wherein the composition comprises at least 5 mcg/g of the beta-cryptoxanthin.132. The composition of claim 127 , wherein the composition comprises at least 1.25 mcg/g of the alpha-carotene.133. The composition of claim 127 , wherein the composition comprises at least 1.0 mcg/g of the cis-beta-carotene.134. The composition of claim 127 , wherein the free fatty acid comprises C16 palmitic acid claim 127 , C18 stearic acid claim 127 , C18-1 oleic acid claim 127 , C18-2 linoleic acid claim 127 , C18-3 linolenic acid claim 127 , or a combination of two or more thereof.135. The ...

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Номер записи: 87
09-03-2017 дата публикации

Microorganisms engineered for increased productivity

Номер: US20170067069A1
Автор: lmad AJJAWI, Nicholas BAUMAN
Принадлежит: Synthetic Genomics Inc

The application provides recombinant microorganisms with increased productivity with respect to control or wildtype microorganisms. The recombinant microorganisms can include a non-native gene encoding a SKP1 polypeptide or a CHORD-derived polypeptide. Increased productivity can be increased biomass or lipid productivity. These recombinant microorganisms can be used to produce products of interest.

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Номер записи: 88
09-03-2017 дата публикации

PRODUCTION OF BIOFUEL FROM TISSUE CULTURE SOURCES

Номер: US20170067083A1
Автор: DIXON Geoffrey Robert, MERRELL William Timothy, Whitton Peter Andrew
Принадлежит:

The present invention provides a method for the production of at least one fatty acid and/or oil from a plant cell suspension culture, the method comprising (i) maintaining a cell suspension culture of oil-producing plant cells under conditions such that the cultured cells synthesise and secrete at least one fatty acid and/or oil into the cell suspension culture medium; and (ii) extracting the thus secreted at least one fatty acid and/or oil from the cell suspension culture medium. 1118-. (canceled)119. A method for the production of at least one fatty acid and/or oil from a non-algal plant cell suspension culture , the method comprising:maintaining a cell suspension culture of oil-producing non-algal plant cells at a pH less than 7.0 such that the cultured cells synthesize and secrete at least one fatty acid and/or oil into the cell suspension culture medium; and(ii) extracting the secreted fatty acid and/or oil from the cell suspension culture medium;{'sub': '2', 'wherein the level of respiratory activity of the non-algal plant cell suspension culture as indicated by Oconsumption, and/or the level of fatty acid and/or oil production of the non-algal plant cell suspension culture, during the extracting step does not drop to less than 80% of the level observed when the extracting step is not being performed, and wherein the non-algal plant cells are not genetically modified.'}120. The method of claim 119 , wherein the non-algal plant cell suspension culture is maintained at a pH less than 7.0 that is suitable to cause fatty acids and/or oils stored in the vacuole of the cultured plant cells to be released claim 119 , via the cytosol of the plant cells claim 119 , into the cell suspension culture medium.121. The method of claim 120 , wherein the cell suspension culture medium is maintained at a selected pH that is less than 7.0 claim 120 , and comprises a buffer that maintains the cell suspension culture medium at around the selected pH.122. The method of claim 119 , ...

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Номер записи: 89
11-03-2021 дата публикации

METHODS OF PRODUCING OMEGA-HYDROXYLATED FATTY ACID DERIVATIVES

Номер: US20210071212A1
Автор: CHANG Cindy, DEL CARDAYRE Stephen B., HOM Louis G., HU Zhihao, Popova Emanuela E., Schirmer Andreas W., Wang Haibo, Zhu Baolong
Принадлежит: GENOMATICA, INC.

The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation. 152.-. (canceled)53. A recombinant microorganism for producing an ω-hydroxy fatty acid derivative in vivo when grown in a fermentation broth in a presence of a carbon source from a renewable feedstock , said microorganism comprising a pathway engineered to express: (a) a thioesterase of EC 3.1.2.-, 3.1.1.5, or 3.1.2.14; or an ester synthase of EC 2.3.1.75 or EC 2.3.1.20; and', '(b) a modified ω-hydroxylase of EC 1.14.15.3; or, '(i) at least two nucleic acid sequences encoding a polypeptide comprising (a) an acyl-ACP reductase of EC 1.2.1.42;', '(b) an alcohol dehydrogenase of EC 1.1.-.-; and', '(c) a modified ω-hydroxylase of EC 1.14.15.3., '(ii) at least three nucleic acid sequences encoding a polypeptide comprising'}54. The recombinant microorganism of claim 53 , wherein the modified ω-hydroxylase of EC 1.14.15.3 comprises a modified cyp153A polypeptide.55. The recombinant microorganism of claim 54 , wherein the modified ω-hydroxylase of EC 1.14.15.3 comprises a cyp153A polypeptide of SEQ ID NO: 2 that is modified to comprise at least the mutation G307A.56. The recombinant microorganism of claim 55 , wherein the modified ω-hydroxylase of EC 1.14.15.3 is a cyp153A polypeptide that is further modified to comprise at least one mutation at an amino acid position selected from the group consisting of 27 claim 55 , 141 claim 55 , 178 claim 55 , 309 claim 55 , 407 claim 55 , and 415.57. The recombinant microorganism of claim 56 , wherein:the mutation at amino acid position 27 is R27L;the mutation at amino acid position 141 is V141I, V141T, V141Q, V141G, V141M, or V141L; ...

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Номер записи: 90
11-03-2021 дата публикации

TWO-STAGE PROCESS FOR PRODUCING OIL FROM MICROALGAE

Номер: US20210071213A1
Автор: Oyler James R.
Принадлежит: Genifuel Corporation

A process for production of biofuels from algae can include cultivating an oil-producing algae by promoting sequential photoautotrophic and heterotrophic growth. The method can further include producing oil by heterotrophic growth of algae wherein the heterotrophic algae growth is achieved by introducing a sugar feed to the oil-producing algae. An algal oil can be extracted from the oil-producing algae, and can be converted to form biodiesel. 117-. (canceled)18. A process for production of biofuels from algae , comprising:a) inducing algae cell body production of an oil-producing algae by photoautotrophic growth;b) initiating heterotrophic growth of the oil-producing algae using a stress induction mechanism;c) inducing algal oil production by heterotrophic growth of the oil-producing algae, wherein the heterotrophic growth includes introducing a sugar feed to the oil-producing algae; andd) extracting an algal oil from the oil-producing algae.19. The method of claim 18 , further comprising converting the oil to biodiesel.20. The process of claim 18 , wherein the oil-producing algae includes a member selected from the group consisting of diatoms (bacillariophytes) claim 18 , green algae (chlorophytes) claim 18 , blue-green algae (cyanophytes) claim 18 , golden-brown algae (chrysophytes) claim 18 , haptophytes claim 18 , and combinations thereof.21Amphipleura, Amphora, Chaetoceros, Cyclotella, Cymbella, Fragilaria, Hantzschia, Navicula, Nitzschia, Phaeodactylum, Thalassiosira, Ankistrodesmus, Botryococcus, Chlorella, Chlorococcum, Dunaliella, Monoraphidium, Oocystis, Scenedesmus, Tetraselmis, Oscillatoria, Synechococcus, Boekelovia. The process of claim 20 , wherein the oil-producing algae includes claim 20 , or combinations thereof.22Chlorella, Dunaliella. The process of claim 21 , wherein the oil-producing algae includes claim 21 , or combinations thereof.23. The process of claim 18 , further comprising introducing a nitrogen-fixing algae to the oil-producing algae ...

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Номер записи: 91
07-03-2019 дата публикации

Method of Producing Lipid

Номер: US20190071698A1
Автор: Sugihara Shinji
Принадлежит: KAO CORPORATION

A method of producing lipids, containing the steps of: 2. A method of modifying the composition of fatty acids , which comprising enhancing the expressions of a gene encoding any one of the following proteins (A) to (F) and a gene encoding an acyl-ACP thioesterase in a transformant , to modify the composition of fatty acids or fatty acids of lipids containing these fatty acids as components produced in a cell of the transformant:(A) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 2;(B) a protein consisting of an amino acid sequence having 90% or more identity with the amino acid sequence of the protein (A), and having acyl-CoA synthetase activity;(C) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 4;(D) a protein consisting of an amino acid sequence having 75% or more identity with the amino acid sequence of the protein (C), and having acyl-CoA synthetase activity;(E) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 6; and(F) a protein consisting of an amino acid sequence having 85% or more identity with the amino acid sequence of the protein (E), and having acyl-CoA synthetase activity.3. The method according to claim 2 , wherein a proportion of the medium-chain fatty acids in the total fatty acids to be produced is increased.4. The method according to claim 1 , wherein the gene encoding any one of the proteins (A) to (F) and the gene encoding the acyl-ACP thioesterase are introduced into a host claim 1 , to enhance the expressions of the genes.5. (canceled)6. The method according to claim 1 , wherein the acyl-ACP thioesterase is a protein consisting of the amino acid sequence set forth in SEQ ID NO: 56 claim 1 , SEQ ID NO: 33 claim 1 , SEQ ID NO: 47 claim 1 , SEQ ID NO: 58 claim 1 , SEQ ID NO: 60 claim 1 , or SEQ ID NO: 62; or a protein consisting of an amino acid sequence having 50% or more identity with the amino acid sequence of the protein claim 1 , and having acyl-ACP thioesterase activity ...

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Номер записи: 92
24-03-2022 дата публикации

ACYL-ACP Reductase With Improved Properties

Номер: US20220090028A1
Автор: Andreas Schirmer, Jacob GANO, Mathew Rude, Na Trinh
Принадлежит: Genomatica Inc

The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.

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Номер записи: 93
19-03-2015 дата публикации

MICROBIAL ENGINEERING FOR THE PRODUCTION OF FATTY ACIDS AND FATTY ACID DERIVATIVES

Номер: US20150079645A1
Автор: Abidi Syed Hussain Imam, Stephanopoulos Gregory
Принадлежит: Massachusetts Institute of Technology

Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production. 1. An isolated oleaginous cell , comprisinga genetic modification that increases expression of one or more genes selected from the group of Hemoglobin, Cytochrome, GLUT, Malic Enzyme, ACC, SCD, FAA1, ACS, ACS2, FAT1, FAT2, PCS60, ACLY, FAS, Acyl-CoA synthetase, Pyruvate carboxylase, and AMPK genes, and/ora genetic modification that decreases expression of JNK2 and/or delta-12 desaturase.210-. (canceled)11. The isolated oleaginous cell of claim 1 , wherein the increased or decreased expression of the gene product confers a beneficial phenotype for the conversion of a carbohydrate source to a fatty acid claim 1 , fatty acid derivative and/or triacylglycerol (TAG) to the cell.12. The isolated oleaginous cell of claim 11 , wherein the beneficial phenotype relative to an unmodified cell of the same type is a modified fatty acid profile claim 11 , a modified TAG profile claim 11 , an increased fatty acid and/or triacylglycerol synthesis rate claim 11 , an increased conversion yield from a carbohydrate source to a fatty acid claim 11 , fatty acid derivative and/or triacylglycerol (TAG) claim 11 , an increased triacylglycerol accumulation in the cell claim 11 , and an increased tolerance of osmotic stress claim 11 , an increased proliferation rate claim 11 , an increased cell volume claim 11 , and/or an increased tolerance of a substance at a concentration lethal to and/or inhibiting proliferation of an unmodified cell of the same type.1316-. (canceled)17. The isolated oleaginous cell of claim 12 , wherein the cell is viable under conditions of osmotic stress lethal to an unmodified cell of the same type.1820-. ( ...

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Номер записи: 94
05-06-2014 дата публикации

SYSTEMS AND METHODS FOR ANAEROBIC DIGESTION AND COLLECTION OF PRODUCTS

Номер: US20140154754A1
Автор: Stephens James
Принадлежит: Blue Marble Energy Corporation

Described herein are methods and systems that can provide independent energy generation as well as systems for the generation of other useful products, such as chemicals. In addition, the systems and methods can provide ways of harnessing the potential of biomass feedstock to generate of multitude of products including green crude, biogas, electricity, heat, fatty acids, biodiesel, ammonia, and chemical products. In many cases, the systems and methods herein utilize anaerobic microorganisms, including aquatic and ruminant organisms, to digest material and create products. 1. A bioreactor system comprising:a first module comprising a first plurality of anaerobic bacteria; anda second module comprising a second plurality of anaerobic bacteria;wherein an output of the first module is in communication with an input of the second module, andwherein the first plurality of anaerobic bacteria comprises a different mixture of anaerobic bacteria than the second plurality of anaerobic bacteria, andwherein the bioreactor system is configured to remove of volatile fatty acids from the first module and is configured to remove ammonia from the second module in order to maintain the pH of the bioreactor system between 5.9 and 8.5.2. The bioreactor system of claim 1 , wherein a majority of the first plurality of anaerobic bacteria is acidogenic bacteria claim 1 , and wherein a majority of the second plurality of anaerobic bacteria is acetogenic bacteria.3. The bioreactor system of claim 1 , wherein a majority of the first plurality of anaerobic bacteria is acidogenic bacteria claim 1 , and wherein a majority of the second plurality of anaerobic bacteria is methanogenic bacteria.4. The bioreactor system of claim 1 , wherein without the removal of volatile fatty acids claim 1 , the pH is not maintained 5.9 and 8.5.5. The bioreactor system of claim 1 , wherein the first and second pluralities of anaerobic bacteria each comprise aquatic anaerobic bacteria and ruminant anaerobic bacteria ...

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Номер записи: 95
05-03-2020 дата публикации

Compounds for Increasing Lipid Synthesis and Storage

Номер: US20200071735A1
Автор: Concetta DiRusso, Nishikant Wase
Принадлежит: NuTech Ventures Inc

This invention relates to methods for increasing lipid accumulation and lipid production in cells. Methods of producing biofuel from cells and preparing nutraceuticals comprising lipids produced according to a method provided herein are also provided.

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Номер записи: 96
22-03-2018 дата публикации

METHODS AND COMPOSITIONS FOR PRODUCING FATTY ALCOHOLS AND FATTY ALDEHYDES

Номер: US20180080053A1
Автор: BRUBAKER Shane, RUDE Mathew A., Schirmer Andreas W.
Принадлежит:

Compositions and methods for producing hydrocarbons such as aldehydes, alkanes, and alkenes are described herein. Certain hydrocarbons can be used in biofuels. 145.-. (canceled)46. A genetically engineered microorganism transformed with an exogenous polynucleotide sequence encoding an aldehyde biosynthetic polypeptide that has at least 80% sequence identity to SEQ ID NO: 66 , 70 , or 72.47. The genetically engineered microorganism of claim 46 , wherein said aldehyde biosynthetic polypeptide catalyzes the conversion of an acyl-ACP to a fatty aldehyde.48. The genetically engineered microorganism of claim 47 , wherein said aldehyde biosynthetic polypeptide is an acyl-ACP reductase.49. The genetically engineered microorganism of claim 46 , wherein said exogenous polynucleotide comprises a recombinant vector.50. The genetically engineered microorganism of claim 46 , wherein said exogenous polynucleotide is stably incorporated into the genomic DNA of the host cell.51. The genetically engineered microorganism of claim 46 , wherein said microorganism is a bacterial cell.52Escherichia. The genetically engineered microorganism of claim 51 , wherein said bacterial cell is an cell or a cyanobacterial cell.53. A genetically engineered microorganism transformed with an exogenous polynucleotide sequence encoding an aldehyde biosynthetic polypeptide comprising:amino acids 126-136 and 138-143 of SEQ ID NO: 66;amino acids 156-159 and 161-175 of SEQ ID NO: 66;amino acids 156-175 of SEQ ID NO: 66;amino acids 126-136 and 138-143 of SEQ ID NO: 70;amino acids 156-159 and 161-175 of SEQ ID NO: 70; oramino acids 156-175 of SEQ ID NO: 70. This application claims the benefit of U.S. Provisional Application No. 61/053,955, filed May 16, 2008, the contents of which are hereby incorporated in their entirety herein.Petroleum is a limited, natural resource found in the Earth in liquid, gaseous, or solid forms. Petroleum is primarily composed of hydrocarbons, which are comprised mainly of carbon ...

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Номер записи: 97
25-03-2021 дата публикации

Acetyl-coa-derived biosynthesis

Номер: US20210087571A1
Автор: Ka-Yiu San, Zhilin Li
Принадлежит: William Marsh Rice University

A method to improve the production of acetyl-CoA-derived biochemicals by overexpression of an acetyl-coenzyme A synthetase or acetate-CoA ligase from naturally acetate-utilizing organisms with or without an added acetate transporter. The production of free fatty acid and its derivatives from renewable carbon source was used as a non-limiting example. Using this approach, the production of free fatty acids with yield close to the maximum theoretical yield at high titer can be achieved. As such, this invention will provide the necessary framework to produce many other products sharing or branching out from the fatty acid synthesis pathway economically. These products include hydrocarbons, fatty alcohols, hydroxy fatty acids, dicarboxylic acids, fatty acid esters, etc.

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Номер записи: 98
31-03-2016 дата публикации

MATERIALS AND METHODS FOR CHARACTERIZING AND USING KASIII FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS

Номер: US20160090576A1
Автор: GARG Shivani, JIN Huanan, Nikolau Basil J., Yandeau-Nelson Marna
Принадлежит:

A mutant , which does not express a functional KASIIIA and/or KASIIIB and method of making; a mutant , which does not express a functional PhaC1, PhaC2, and/or PhaC3 and method of making; method of characterizing substrate specificity of KASIII; method of making mutant KASIII with altered substrate specificity and/or altered level of activity and nucleic acid, vector, host cell/organism, and mutant KASIII; an in vitro, high-throughput spectrophotometric method of assaying KASIII activity, and materials and methods for using KASIII for production of bi-functional fatty acids and the materials so produced. 1Bacillus subtilis. A mutant , which does not express a functional 3-ketoacyl-acyl carrier protein (ACP) synthase III (KASIII) selected from the group consisting of KASIIIA and KASIIIB.251.-. (canceled)52B. subtilisB. subtilis. A method of making the mutant of claim 1 , which method comprises introducing into wild-type a mutation selected from the group consisting of a mutation that prevents expression of a functional KASIIIA and a mutation that prevents expression of a functional KASIIIB.53Rhodospirillum rubrum. A mutant claim 1 , which does not express a functional polyhydroxyalkanoate (PHA) polymerase selected from the group consisting of PhaC1 claim 1 , PhaC2 claim 1 , and PhaC3.54R. rubrumR. rubrum. A method of making the mutant of claim 53 , which method comprises introducing into wild-type a mutation selected from the group consisting of a mutation that prevents expression of a functional PhaC1 claim 53 , a mutation that prevents expression of a functional PhaC2 claim 53 , and a mutation that prevents expression of a functional PhaC3.55B. subtilisB. subtilis. A method of characterizing substrate specificity of a KASIII claim 1 , which method comprises expressing the KASIII claim 1 , which is not expressed in wild-type claim 1 , in the mutant of claim 1 , which does not express a functional KASIIIA and a functional KASIIIB claim 1 , and assessing the ...

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Номер записи: 99
02-04-2015 дата публикации

Biotransformation using genetically modified candida

Номер: US20150094483A1
Автор: Jeremy Minshull, Jon E. Ness
Принадлежит: Jeremy Minshull, Jon E. Ness

A substantially pure Candida host cell is provided for the biotransformation of a substrate to a product wherein the host cell is characterized by a first genetic modification class that comprises one or more genetic modifications that collectively or individually disrupt at least one alcohol dehydrogenase gene in the substantially pure Candida host cell.

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Номер записи: 100