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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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05-01-2012 дата публикации

Diagnosing fetal chromosomal aneuploidy using massively parallel genomic sequencing

Номер: US20120003635A1
Автор: Kwan Chee Chan, Rossa Wai Kwun Chiu, Yuk-Ming Dennis Lo
Принадлежит: Individual

Embodiments of this invention provide methods, systems, and apparatus for determining whether a fetal chromosomal aneuploidy exists from a biological sample obtained from a pregnant female. Nucleic acid molecules of the biological sample are sequenced, such that a fraction of the genome is sequenced. Respective amounts of a clinically-relevant chromosome and of background chromosomes are determined from results of the sequencing. The determination of the relative amounts may count sequences of only certain length. A parameter derived from these amounts (e.g. a ratio) is compared to one or more cutoff values, thereby determining a classification of whether a fetal chromosomal aneuploidy exists. Prior to sequencing, the biological sample may be enriched for DNA fragments of a particular sizes.

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Номер записи: 1
05-01-2012 дата публикации

Diagnosing fetal chromosomal aneuploidy using massively parallel genomic sequencing

Номер: US20120003637A1
Автор: Kwan Chee Chan, Rossa Wai Kwun Chiu, Yuk-Ming Dennis Lo
Принадлежит: Chinese University of Hong Kong CUHK

Embodiments of this invention provide methods, systems, and apparatus for determining whether a fetal chromosomal aneuploidy exists from a biological sample obtained from a pregnant female. Nucleic acid molecules of the biological sample are sequenced, such that a fraction of the genome is sequenced. Respective amounts of a clinically-relevant chromosome and of background chromosomes are determined from results of the sequencing. The determination of the relative amounts may count sequences of only certain length. A parameter derived from these amounts (e.g. a ratio) is compared to one or more cutoff values, thereby determining a classification of whether a fetal chromosomal aneuploidy exists. Prior to sequencing, the biological sample may be enriched for DNA fragments of a particular sizes.

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Номер записи: 2
05-01-2012 дата публикации

Genetic polymorphisms in the prostate-specific antigen gene promoter

Номер: US20120003647A1
Автор: Anuradha Rao, Bao-li CHANG, Jianfeng Xu, Scott D. Cramer
Принадлежит: Individual

The present invention includes methods of identifying a subject at risk for increased cellular PSA production and/or prostate cancer by detecting the presence or absence of a genetic polymorphism in the prostate specific antigen gene.

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Номер записи: 3
05-01-2012 дата публикации

Gene expression markers of oncolytic virus sensitivity

Номер: US20120004119A1
Автор: Lynda Chin, Marc Lenburg
Принадлежит: Lynda Chin, Marc Lenburg

A method of predicting an efficacy of an oncolytic virus treatment for a tumor comprises calculating a single-gene predictor score for each of a plurality of genes; calculating a predictor score from the sum of the single-gene predictor scores for the plurality of genes; and predicting, if the predictor score is greater than a predictor score threshold, that the treatment would have efficacy, and if the predictor score is less than a predictor score threshold, that the treatment would lack efficacy. Also, methods of predicting an efficacy of a treatment for a tumor comprise identifying the type and subtype of the tumor, wherein the efficacy of the treatment for the type and subtype of the tumor is known.

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Номер записи: 4
12-01-2012 дата публикации

Methods of predicting responsiveness to interferon treatment and treating hepatitis c infection

Номер: US20120009148A1
Автор: Yoav Smith
Принадлежит: Yissum Research Development Co of Hebrew University of Jerusalem

Provided are methods of predicting responsiveness of a subject to interferon treatment, comprising comparing a level of expression in a cell of the subject of at least one gene selected from the group consisting KIR3DL3, KIR3DL2, KIR3DL1, KIR2DL1, KIR2DL2, KIR2DL3, KLRG1, KIR3DS1, CD160, HLA-A, HLA-B, HLA-C, HLA-F, HLA-G and IFI27 to a reference expression data of the at least one gene obtained from at least one interferon responder subject and/or at least one interferon non-responder subject. Also provided are methods and pharmaceutical compositions for treating a subject in need of interferon treatment.

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Номер записи: 5
12-01-2012 дата публикации

Renal cell carcinoma biomarkers

Номер: US20120009201A1
Автор: Bih-Rong Wei, Donald J. Johann, Josip Blonder, Timothy D. Veenstra
Принадлежит: US Department of Health and Human Services

Disclosed herein is a method of identifying a tumor biomarker. In one example, a tumor biomarker is identified by obtaining a peripheral biological fluid sample from a subject with a tumor as well as a tumor sample and an adjacent non-tumor sample from such subject. A protein expression profile is detected in the peripheral biological fluid sample, tumor sample and adjacent non-tumor sample. The protein expression profiles of the peripheral biological fluid sample, tumor sample and adjacent non-tumor sample are then compared, wherein an increase in expression of a specific protein in the tumor sample and peripheral biological fluid sample but not in the adjacent non-tumor sample indicates that the specific protein is a biomarker of the tumor. Also disclosed herein is a gene profiling signature that can be used to diagnosis a subject with renal cell carcinoma (RCC) or to identify agents with therapeutic potential to treat RCC. Thus, methods of diagnosing a subject with RCC are disclosed. Methods are also provided for identifying agents that alter an activity of a RCC biomarker.

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Номер записи: 6
12-01-2012 дата публикации

Tmprss2 for the diagnosis of prostate disease

Номер: US20120009571A1
Автор: Heather R. Sanders, Maher Albitar
Принадлежит: Quest Diagnostics Investments LLC

Described herein are methods, compositions and kits directed to the detection the 5′ portion of TMPRSS2 mRNA for the detection and diagnosis of prostate disease including prostate cancer and benign prostatic hyperplasia.

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Номер записи: 7
12-01-2012 дата публикации

Inducible nucleic acid targets for detection of pathogens, methods and compositions thereof

Номер: US20120009575A1
Автор: Craig Cummings, Hemant Pawar, Manohar Furtado, Olga Petrauskene, Priya Balachandran, Robert Tebbs
Принадлежит: Life Technologies Corp

The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.

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Номер записи: 8
12-01-2012 дата публикации

Probe for Detection of Polymorphism in ABL Gene, and Use Thereof

Номер: US20120009576A1
Автор: Toshiya Hosomi
Принадлежит: Arkray Inc

A probe for detecting a polymorphism in ab1 gene, said probe comprising at least one fluorescence-labeled oligonucleotide selected from P1 below: (P1) an oligonucleotide comprising a nucleotide sequence complementary to a nucleotide sequence of 10 to 50 consecutive nucleotides containing nucleotides 125 to 133 in SEQ ID NO:1 or 2 or a homologous sequence thereof, wherein the nucleotide corresponding to the nucleotide at position 125 is cytosine and labeled with a fluorescent dye.

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Номер записи: 9
12-01-2012 дата публикации

Methods for determining fraction of fetal nucleic acids in maternal samples

Номер: US20120010085A1
Автор: David A. COMSTOCK, Gabrielle HEILEK, Manjula CHINNAPPA, Michael Hunkapiller, Richard P. Rava, Yue-Jen Chuu
Принадлежит: Artemis Health Inc, Verinata Health Inc

The invention provides compositions and methods for determining the fraction of fetal nucleic acids in a maternal sample comprising a mixture of fetal and maternal nucleic acids. The fraction of fetal nucleic acids can be used in determining the presence or absence of fetal aneuploidy.

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Номер записи: 10
12-01-2012 дата публикации

Novel uses of vegfxxxb

Номер: US20120010138A1
Автор: Andrew Salmon, David Owen Bates, Steven James Harper
Принадлежит: University of Bristol

The invention provides VEGF xxx b, or an agent which selectively promotes the expression of VEGF xxx b in preference to VEGF xxx in cells of a subject or in vitro, or an expression vector system which causes the expression of the VEGF xxx b in a host organism, for use in treating or preventing microvascular hyperpermeability disorders, or in regulating the pro-angiogenic pro-permeability properties of VEGF xxx isoforms, or in supporting epithelial cell survival without increased permeability, or in reducing the nature (for example the number density and/or size) of fenestrations of epithelial filtration membranes in vivo or in vitro.

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Номер записи: 11
19-01-2012 дата публикации

Influenza virus detection and diagnosis

Номер: US20120015346A1
Автор: Doris J. Bucher, Manojkumar Ramanunninair
Принадлежит: New York Medical College

The invention discloses compositions comprising nucleic acid(s) for rapid detection, identification, differentiation, and/or diagnosis of certain Influenza virus A subtypes, e.g., H1N1, H3N2 and A(2009 H1N1)pdm, and methods of use thereof, e.g., Short-run RT-PCR, including an RT-PCR assay kit, comprising the disclosed composition(s).

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Номер записи: 12
19-01-2012 дата публикации

Compositions and methods for detection of hepatitis a virus nucleic acid

Номер: US20120015348A1
Автор: James D. Carlson, Steven T. Brentano
Принадлежит: Gen Probe Inc

Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.

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Номер записи: 13
19-01-2012 дата публикации

Compositions and methods for detecting cryptococcus neoformans

Номер: US20120015362A1
Автор: Eli Mordechai, John Entwistle, Martin Adelson, Melanie Feola
Принадлежит: Medical Diagnostic Laboratories LLC

Disclosed are oligonucleotides useful in methods for determining whether a sample contains Cryptococcus neoformans , a causative agent for human cryptococcosis. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fungal specific transcription factor gene in Cryptococcus neoformans , are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Cryptococcus neoformans . Real-time PCR and detection using florescence resonance energy transfer is disclosed.

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Номер записи: 14
19-01-2012 дата публикации

Methods and compositions to identify increased risk of breast cancer by detection of cpg island methylator phenotype (cimp)

Номер: US20120015366A1
Автор: Victoria L. Seewaldt
Принадлежит: Duke University

The present invention is directed to kits and methods for identifying a female subject as having an increased risk of developing breast cancer, comprising detecting a CPG island methylator phenotype (CIMP) in nucleic acid of the subject.

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Номер записи: 15
19-01-2012 дата публикации

Microrna profiles for evaluating multiple sclerosis

Номер: US20120015830A1
Автор: Douglas W. Bigwood, Eric M. Eastman, Michael Elashoff
Принадлежит: DIOGENIX Inc

The present invention provides methods, systems, and kits for evaluating multiple sclerosis (MS) in a patient. Particularly, the invention provides convenient miRNA-based tests for evaluating a patient for MS, including for diagnosing MS, for excluding MS as a diagnosis, for determining the presence of disease activity associated with MS, and for monitoring the course of disease or efficacy of treatment for MS.

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Номер записи: 16
26-01-2012 дата публикации

DOPAMINERGIC NEURON PROLIFERATIVE PROGENITOR CELL MARKER Nato3

Номер: US20120021417A1
Автор: Tomoya Nakatani, Yasuko Nakagawa, Yoshimasa Sakamoto, Yuichi Ono
Принадлежит: Eisai R&D Management Co Ltd

The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of a Nato3 gene, or a complementary sequence thereto, and an antibody against a Nato3 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.

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Номер записи: 17
26-01-2012 дата публикации

Genetic markers for risk management of atrial fibrillation and stroke

Номер: US20120021989A1
Автор: Daniel Gudbjartsson, Hilma Holm
Принадлежит: DECODE GENETICS EHF

The invention relates to procedures and methods of determining a susceptibility to cardiac arrhythmia, including Atrial Fibrillation, Atrial Flutter and Stroke, by assessing the presence or absence of alleles at polymorphic markers found to be associated with risk of these conditions. The invention further relates to kits encompassing reagents for assessing such markers, and diagnostic 5 methods, uses and procedures for utilizing such susceptibility markers.

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Номер записи: 18
02-02-2012 дата публикации

Method and compositions for cancer prognosis

Номер: US20120027750A1
Автор: Guillaume Cartron, Hervé WATIER
Принадлежит: Centre Hospitalier Regional Universitaire De Tours, UNIVERSITE FRANCOIS RABELAIS DE TOURS

Methods are provided to determine the cancer prognosis of subjects and/or to adapt the treatment protocol of subjects having or susceptible to cancer. Embodiments include the steps of determining in vitro the genotype of said subject at a polymorphism in the C3-ITGAM axis, making a cancer prognosis of the subject based on said genotype and selecting an anti-cancer treatment for the subject.

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Номер записи: 19
02-02-2012 дата публикации

Compositions and methods for treating or preventing inflammatory bowel disease and colon cancer

Номер: US20120027799A1
Автор: Cynthia L. Sears, Drew M. Pardoll, Franck Housseau, Shaoguang Wu
Принадлежит: JOHNS HOPKINS UNIVERSITY

The invention provides compositions and methods for useful for the diagnosis of inflammatory bowel disease, ETBF-induced colitis, colonic hyperplasia and/or colon carcinogenesis in a subject in biological samples (e.g., stool, urine, blood, serum, tissue). The invention further provides compositions and methods for the treatment or prevention of colitis, colon cancer, or inflammatory bowel disease (e.g., Crohn's disease).

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Номер записи: 20
02-02-2012 дата публикации

Galectin-1 (gal1) as a biomarker for differential diagnosis of osteosarcoma and chondrosarcoma

Номер: US20120028825A1
Автор: Anne Gomez-Brouchet, Claudine Schiff
Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS, Institut National de la Sante et de la Recherche Medicale INSERM, UNIVERSITE de la MEDITERRANNEE

The present invention relates to a method for differential diagnosis of osteosarcoma and chondrosarcoma, especially chondroblastic osteosarcoma and conventional chondrosarcoma, in a patient comprising a step consisting of detecting galectin-1 (GAL1) expression in a bone sample obtained from said patient.

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Номер записи: 21
02-02-2012 дата публикации

Method for determining a risk, for a subject, of suffering from atopic dermatitis or severity of atopic dermatitis for a subject suffering from atopic dermatitis and method for using a single-nucleotide polymorphism rs12313273 as a biomarker for determining the development or severity of atopic dermatitis

Номер: US20120028827A1
Автор: Chih-Hung Lee, Hsin-Su Yu, Suh-Hang Juo, Wei-Chiao Chang, Wei-Chiao Chen
Принадлежит: KAOHSIUNG MEDICAL UNIVERSITY

The invention provides a method for determining a risk, for a subject, of suffering from atopic dermatitis, including: obtaining a biosample of the subject; detecting the presence of the single-nucleotide polymorphism rs12313273 (C/T) at position 30881 of the ORAI1 gene (SEQ ID No.: 1) in the biosample; and determining the risk, for the subject, of suffering from atopic dermatitis, wherein the presence of a C allele or genotype CC of the single-nucleotide polymorphism rs12313273 (C/T) indicates that the subject is at increased risk for suffering from atopic dermatitis.

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Номер записи: 22
09-02-2012 дата публикации

Marker for Liver-Cancer Diagnosis and Recurrence and Survival Prediction, a Kit Comprising the Same, and Prognosis Prediction in Liver-Cancer Patients Using the Marker

Номер: US20120034235A1
Автор: Chul Ju Han, Dong Hyoung Lee, Eun Ju LEE, Eun Ran Park, Hyun Jin Shin, Kee Ho Lee, Myoung Jin Park, Pu Hyeon Cha, Sang Bum Kim, Sang Gu Hwang, Seon Rang Woo, Sun Hoo Park, Yong Ho Ham
Принадлежит: Korea Institute of Radiological and Medical Sciences

A composition for detecting a marker for the diagnosis or prognosis of liver cancer is disclosed. The composition includes an agent capable of assessing the expression level of UQCRH (ubiquinol-cytochrome c reductase hinge protein). In addition, a kit having the composition, a microarray for the diagnosis of liver cancer using the marker, and a method for detecting the marker, and predicting recurrence following surgery in liver cancer patients are disclosed. The marker is able to contribute to the early diagnosis of liver cancer and prediction of recurrence following surgery and survival of liver cancer patients who have undergone hepatic resection, and also is significant for being a promising therapeutic target for liver cancer.

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Номер записи: 23
09-02-2012 дата публикации

Compositions and methods for the treatment of immune related diseases

Номер: US20120035073A1
Автор: Brisdell Hunte, Hilary Clark, Janet K. Jackman, Jill R. Schoenfeld, P. Mickey Williams, Sarah C. Bodary-Winter, Thomas D. Wu, William I. Wood
Принадлежит: Individual

The present invention relates to compositions containing novel proteins and methods of using those compositions for the diagnosis and treatment of immune related diseases.

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Номер записи: 24
09-02-2012 дата публикации

Dual targeting of mir-208 and mir-499 in the treatment of cardiac disorders

Номер: US20120035243A1
Автор: Eric Olson, Eva Van Rooij
Принадлежит: University of Texas System

The present invention provides a method of treating or preventing cardiac disorders in a subject in need thereof by inhibiting the expression or function of both miR-499 and miR-208 in the heart cells of the subject. In particular, specific protocols for administering inhibitors of the two miRNAs that achieve efficient, long-term suppression are disclosed. In addition, the invention provides a method for treating or preventing musculoskeletal disorders in a subject in need thereof by increasing the expression or activity of both miR-208 and miR-499 in skeletal muscle cells of the subject.

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Номер записи: 25
09-02-2012 дата публикации

Predictive models and method for assessing age

Номер: US20120036101A1
Автор: James A. Winrove, Michael R. Elashoff, Steve Rosenberg, Whittemore G. Tingley
Принадлежит: CardioDX Inc

Biomarkers useful for diagnosing and assessing physiological age are provided, along with kits for measuring their expression. The invention also provides predictive models, based on the biomarkers, as well as computer systems, and software embodiments of the models for scoring and optionally classifying samples. In a preferred embodiment, the biomarkers include a group of biomarkers whose expression levels are highly correlated to each other. In a preferred embodiment, expression levels of CD248; CD248 and SLC 1A7; CD248 and one, two, three or four of the group consisting of CCR7, B3GAT1, VSIG4 and LR-RN3; or CD248, SLC1A7 and one, two, three or four of the group consisting of CCR7, B3GAT1, VSIG4 and LRRN3 are determined.

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Номер записи: 26
16-02-2012 дата публикации

Genetic polymorphisms associated with venous thrombosis, methods of detection and uses thereof

Номер: US20120039864A1
Автор: Frits R. Rosendaal, Irene D. Bezemer, James J. Devlin, Lance Bare, Pieter H. Reitsma
Принадлежит: Celera Corp, Leids Universitair Medisch Centrum LUMC

The present invention is based on the discovery of genetic polymorphisms that are associated with coronary heart disease and in particular VT and response to drug treatment. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection.

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Номер записи: 27
16-02-2012 дата публикации

Compositions and methods for diagnosing prostate cancer based on detection of slc45a3-elk4 fusion transcript

Номер: US20120039887A1
Автор: David S. Rickman, Dorothee Pflueger, Mark A. Rubin
Принадлежит: CORNELL UNIVERSITY

RNA transcripts representing a fusion of a human SLC45A3 nucleic acid and a human ELK4 nucleic acid that are associated with prostate cancer are described. Compositions and methods useful for detection of fusion transcripts of human SLC45A3 and ELK4 genetic sequences associated with cancer and useful for cancer therapy are provided.

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Номер записи: 28
16-02-2012 дата публикации

RBM3 in Testicular Cancer Diagnostics and Prognostics

Номер: US20120040338A1
Автор: Jakob Eberhard, Karin Jirstrom
Принадлежит: Atlas Antibodies AB

The present disclosure provides a method for determining whether a mammalian subject belongs to a first or a second group, wherein subjects of the first group have a higher risk of having a testicular disorder than subjects of the second group, comprising the steps of: evaluating an amount of RBM3 protein or RBM3 mRNA in at least part of an earlier obtained sample comprising biological material from a testicle of said subject and determining a sample value corresponding to the evaluated amount; comparing said sample value with a predetermined reference value; and if said sample value is higher than said reference value, concluding that the subject belongs to the first group; and if said sample value is lower than or equal to said reference value, concluding that the subject belongs to the second group. Further, a prognostic method for testicular cancer is provided, as well as means and uses with prognostic and diagnostic applications.

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Номер записи: 29
16-02-2012 дата публикации

Primers, probes and methods for nucleic acid amplification

Номер: US20120040352A1
Автор: Arthur Reis, Cristina Hartshorn, J. Aquiles Sanchez, Jesse Salk, John Rice, Kenneth Pierce, Lawrence J. Wangh
Принадлежит: BRANDEIS UNIVERSITY

Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.

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Номер записи: 30
16-02-2012 дата публикации

Biomarkers for stroke

Номер: US20120040858A1
Автор: Byron D. Ford, Gregory Ford
Принадлежит: Morehouse School of Medicine Inc

Biomarkers for stroke and methods for their detection are disclosed. In one aspect, the present application discloses biomarkers for the diagnosis of stroke in a subject. In another aspect, the application discloses a method for the diagnosis of stroke in a subject. The method comprises detection of stroke biomarkers in cerebrospinal fluid, blood, serum or PMBCs of a subject. Also disclosed is a kit for the detection of biomarkers for the diagnosis of stroke in a subject.

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Номер записи: 31
23-02-2012 дата публикации

Detecting bcl-b expression in cancer and uses thereof

Номер: US20120046195A1
Автор: John C. Reed, Maryla Krajewska
Принадлежит: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE

Provided herein are compositions and methods of detecting Bcl-B expression in cancer cells to prognose, monitor, or select therapies for cancers such as breast cancer, prostate cancer, lung cancer, or gastric cancer.

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Номер записи: 32
01-03-2012 дата публикации

Markers and methods for assessing and treating lupus patients susceptible to photoprovocation

Номер: US20120052066A1
Автор: Cesar Calderon, John Getsy
Принадлежит: Centocor Inc

A method for predicting or detecting susceptibility to lupus of an individual subjected to photoprovocation obtains biological samples from the individual before and after exposure to photoprovocation and compares the levels of at least a portion of members of a 45-member panel or subset thereof to determine whether the individual is susceptible to lupus. The method enables identification of potential lupus patients prior to onset of disease symptoms.

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Номер записи: 33
01-03-2012 дата публикации

Compositions, Kits, and Methods for Predicting Anti-Cancer Response to Anthracyclines

Номер: US20120052079A1
Автор: Andrea L. Richardson, Zhigang C. Wang
Принадлежит: Dana Farber Cancer Institute Inc

The present invention is based, in part, on the discovery that amplification of human chromosome 8q22-23 regions and over-expression of 8q22-23 genes (e.g., LAPTM4B and YWHAZ) is associated with and predictive of resistance to anthracycline-type chemotherapy. Accordingly, the invention relates to compositions, kits, and methods for predicting the response of cancer cells, e.g., breast, prostate, lung, ovarian, pancreatic, liver, and colon malignancies to anthracyclines.

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Номер записи: 34
01-03-2012 дата публикации

OLIGONUCLEOTIDES FOR DETECTING E. coli O157:H7 STRAINS AND USE THEREOF

Номер: US20120052494A1
Автор: Jason Opdyke, Jun Li, Win Den Cheung
Принадлежит: Samsung Techwin Co Ltd

Oligonucleotides, a kit, and a method for detecting E. coli O157:H7 strains are provided. According to the kit for detecting E. coli O157:H7 strains and the method of detecting E. coli O157:H7 strains by using the kit, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time.

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Номер записи: 35
01-03-2012 дата публикации

Genetic markers and diagnostic methods for resistance of breast cancer to hormonal therapies

Номер: US20120052508A1
Автор: Erhan Bilal, Gyan Bhanot, Shrider Ganesan
Принадлежит: Rutgers State University of New Jersey

This application provides a method to identify genetic markers associated with increased sensitivity or resistance to hormonal therapies using an outlier analysis. More specifically, this application discloses that amplifications on chromosomes 8 and 17 are associated with increased proliferation and poor outcome in ER-positive breast cancer, and amplicons 17q21.33-q25.1, 8p11.2 and 8q24.3 may be responsible for higher proliferation and poor outcome in the setting of antiestrogen, in particular Tamoxifen, treatment clinically observed in a subset of ER-positive, HER2-negative breast cancers. The invention also provides use of the identified genetic markers in the development of targeted treatments for antiestrogen-resistant ER-positive breast cancers as well as in improving current methods of drug response prediction.

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Номер записи: 36
01-03-2012 дата публикации

Genetic Risk Analysis In Reward Deficiency Syndrome

Номер: US20120053070A1
Автор: Kenneth Blum, Roger L. Waite, William B. Downs, William J. Heaney
Принадлежит: Individual

Methods and kits for performing genetic analyses of biological samples to assess predisposition for and/or to stratify risk Reward Deficiency Syndrome (RDS) behavior, including various addictive and/or compulsive behaviors, are described. The methods and kits of the invention use different nucleic acid probes to determine if a human subject's genome contains one or more alleles for one or more genes implicated in RDS.

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Номер записи: 37
01-03-2012 дата публикации

Methods of predicting medically refractive ulcerative colitis (mr-uc) requiring colectomy

Номер: US20120053131A1
Автор: Dermot P. McGovern, Jerome I. Rotter, Kent D. Taylor, Stephan R. Targan, Talin Haritunians, Xiuqing Guo
Принадлежит: Cedars Sinai Medical Center

Disclosed are methods of predicting the development of medically refractory ulcerative colitis (MR-UC) in a patient In one embodiment, disclosed is a method of prognosing ulcerative colitis in an individual by determining the presence or absence of one or more risk variants, where the presence of one or more risk variants is indicative of a severe and/or aggressive form of ulcerative colitis. In another embodiment, the severe form of ulcerative colitis is indicative of MR-UC.

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Номер записи: 38
01-03-2012 дата публикации

ISOLATED GENOMIC POLYNUCLEOTIDE FRAGMENTS FROM THE p15 REGION OF ISOLATED GENOMIC POLYNUCLEOTIDE FRAGMENTS FROM THE p15 REGION OF CHROMOSOME 11 ENCODING HUMAN TUMOR SUPPRESSING SUBTRANSFERABLE CANDIDATE 4 (TSSC4)

Номер: US20120053232A1
Автор: James W. Ryan
Принадлежит: RYOGEN LLC

Provided herein are isolated genomic polynucleotide fragments from the from the p15 region of chromosome 11 encoding human and tumor suppressing subtransferable candidate 4 (TSSC4) and methods of use.

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Номер записи: 39
01-03-2012 дата публикации

Corn Variety DE811ASR(BC5)

Номер: US20120054921A1
Автор: Alexandre Da Silva Conceicao, Dilbag Singh Multani, Elizabeth S. Jones, James A. Hawk, Jennifer S. Jaqueth, Karen E. Broglie, Karlene H. Butler, Marymar G. Butruille, Petra Johanna Christina Cecilia Wolters, Travis James Frey
Принадлежит: EI Du Pont de Nemours and Co, PIONEER HI BRED INTERNATIONAL INC, University of Delaware

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum , which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum , and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

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Номер записи: 40
08-03-2012 дата публикации

Compositions and methods for detecting klebsiella pneumoniae

Номер: US20120058487A1
Автор: Ann Huletsky, Christian Menard, François J. Picard, Marc Ouellette, Maurice Boissinot, Michel G. Bergeron, Paul H. Roy
Принадлежит: GeneOhm Sciences Canada Inc

Four highly conserved genes, encoding translation elongation factor Tu, translation elongation factor G, the catalytic subunit of proton-translocating ATPase and the RecA recombinase, are used to generate species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes and amplification primers to rapidly detect and identify algal, archaeal, bacterial, fungal and parasitical pathogens from clinical specimens for diagnosis. The detection of associated antimicrobial agents resistance and toxin genes are also under the scope of the present invention.

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Номер записи: 41
08-03-2012 дата публикации

METHOD OF DIAGNOSING POOR SURVIVAL PROGNOSIS COLON CANCER USING miR-203

Номер: US20120058914A1
Автор: Aaron J. Schetter, Carlo M. Croce, Curtis C. Harris
Принадлежит: Ohio State University Research Foundation, US Department of Health and Human Services

The present invention provides novel methods and compositions for the diagnosis and treatment of colon cancers. In particular, the present invention provides diagnostics and prognostics for colon (including colon adenocarcinoma) cancer patients, wherein the methods related to measuring miR levels can predict poor survival. The invention also provides methods of identifying inhibitors of tumorigenesis.

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Номер записи: 42
15-03-2012 дата публикации

Progesterone receptor membrane component 1 (PGRMC1) as an indicator of fertility in animals

Номер: US20120060767A1
Автор: John J. Peluso
Принадлежит: University of Connecticut

Methods of evaluating fertility of an animal based on determination of a PGRMC1 characteristic that correlates with animal fertility. In one embodiment, a sample is obtained from an animal, a PGRMC1 characteristic is determined and compared to a baseline PGRMC1 characteristic, wherein a variation between the determined PGRMC1 characteristic and the baseline characteristic indicates a level of fertility of the animal. A PGRMC1 characteristic can be one or more of PGRMC1 expression, transcription, translation, amino acid sequence, nucleic acid sequence, post-translational modification, cell localization or tissue localization. A sample can be a cell sample, a tissue sample, a blood sample, a lymphocyte sample, an oocyte sample, or a sperm sample. The animal can be male or female. In one embodiment, a variation of PGRMC1 nucleic acid sequence indicates reduced fertility of the animal.

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Номер записи: 43
15-03-2012 дата публикации

Modified nucleotide and real-time polymerase reaction using the same

Номер: US20120064531A1
Автор: Dae-Ro AHN
Принадлежит: Korea Advanced Institute of Science and Technology KAIST

The present invention relates to a modified nucleotide and real-time polymerase reaction using the nucleotide. Specifically, the present invention relates to a fluorescence material linked-nucleotide, a composition for real-time polymerase reaction comprising the nucleotide, an analysis kit and an analysis method. In the present invention, the fluorescence material linked-nucleotide serves the dual roles of producing fluorescence signal as well as being used as a substrate. Therefore, the present invention is economically advantageous because it is unnecessary to prepare probes, but can be applied to analyze various real-time polymerase reactions such as PCR, RCA and isothermal polymerization reaction, and shows higher quality of performance than the past methods.

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Номер записи: 44
15-03-2012 дата публикации

Methods of Diagnosing Hypophosphatemic Disorders

Номер: US20120064544A1
Автор: Ken White, Michael Econs, Thomas Meitinger, Tim Matthias Strom
Принадлежит: Indiana University Research And Technology Corp, Ludwig Maximilians Universitaet Muenchen LMU

The present invention relates to methods of diagnosing hypophosphatemic disorders.

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Номер записи: 45
15-03-2012 дата публикации

Methods for the diagnosis of fetal disease

Номер: US20120065076A1
Автор: David G. Peters, Tianjiao Chu
Принадлежит: University of Pittsburgh

Methods are provided for detecting an aneuploidy in a fetus. These methods can be used to detect trisomy 13, 8 or 21, amongst other aneupoloidies. In some embodiments, the methods include selectively purifying fetal DNA from a maternal biological sample using the methylation status of a CpG containing genomic sequence and genotyping the fetus using the purified fetal DNA, thereby detecting aneuploidy in the fetus.

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Номер записи: 46
15-03-2012 дата публикации

Methods and kits for diagnosing osteoarthritis and predicting progression

Номер: US20120065094A1
Автор: Mukundan Attur, Steven B. Abramson
Принадлежит: New York University NYU

This disclosure relates to methods and kits for diagnosing osteoarthritis and for determining the progression of osteoarthritis in a subject.

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Номер записи: 47
15-03-2012 дата публикации

Predicting risk of major adverse cardiac events

Номер: US20120065897A1
Автор: Eugene R. Heyman, James V. Snider
Принадлежит: Critical Care Diagnostics Inc

Measurement of circulating ST2 and natriuretic peptide (e.g., NT-proBNP) concentrations is useful for the prognostic evaluation of subjects, in particular for the prediction of adverse clinical outcomes, e.g., mortality, transplantation, and heart failure.

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Номер записи: 48
15-03-2012 дата публикации

Identification of a jak2 mutation involved in vaquez polyglobulia

Номер: US20120066776A1
Автор: Chloe James, Jean-Pierre Le Couedic, Nicole Casadevall, Valerie Ugo, William Vainchenker
Принадлежит: Assistance Publique Hopitaux de Paris APHP, IGR&D SA, Institut National de la Sante et de la Recherche Medicale INSERM

The present invention concerns the V617F variant of the protein-tyrosine kinase JAK2, said variant being responsible for Vaquez Polyglobulia. The invention also relates to a first intention diagnostic method for erythrocytosis and thrombocytosis allowing their association with myeloproliferative disorders, or to the detection of the JAK2 V617F variant in myeloproliferative disorders allowing their reclassification in a new nosological group.

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Номер записи: 49
15-03-2012 дата публикации

Test agent for visceral obesity and use thereof

Номер: US20120066777A1
Автор: Atsunori Kashiwagi, Hiroshi Maegawa, Masatomo Rokushima, Minoru Ikeda, Minoru Suzuki, Shiro Maeda, Tatsuya Takahashi, Tohru Tani, Yoshihiro Kawamura
Принадлежит: RIKEN Institute of Physical and Chemical Research, Shiga University of Medical Science NUC, Shionogi and Co Ltd

Disclosed are: a method for detecting (diagnosing) visceral obesity in a subject; a test agent useful for the method; a method for searching for a substance that can be used as an active ingredient for ameliorating visceral obesity; and an ameliorating agent for visceral obesity or a medicinal agent for preventing a metabolic disease developed as a result of the progression of visceral obesity. As the test agent, a polynucleotide which comprises at least 15 nucleotides and can hybridize with a nucleotide sequence for coiled-coil domain containing protein 3 (CCDC3) gene or a nucleotide sequence complementary to the nucleotide sequence under stringent conditions or an antibody capable of recognizing CCDC3 protein is used.

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Номер записи: 50
22-03-2012 дата публикации

Treatment of pancreatic cancer using a dr5 agonist in combination with gemcitabine

Номер: US20120070432A1
Автор: Jeffrey Scott Wiezorek, Jennifer Joy Kordich, Jonathan David Graves
Принадлежит: AMGEN INC

Methods and compositions for treatment of exocrine pancreatic cancer in a human patient comprising administering a therapeutically effective amount of a DR5 agonist and gemcitabine. Methods and compositions for treating a patient by identifying the alleleic variant of FcγRIIIA.

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Номер записи: 51
22-03-2012 дата публикации

Biomarkers for differentiating melanoma from benign nevus in the skin

Номер: US20120071343A1
Автор: Xiao-Jun Ma, Xingyong Wu, Yuling Luo
Принадлежит: Advanced Cell Diagnostics Inc

Disclosed is a method for diagnosing melanoma in a human subject, as well as a method for providing a prognosis to a human subject who is at risk of developing melanoma recurrence, and a method for determining the stage of melanoma in a human subject, comprising the step of determining the level of expression of phosphatase and actin regulator 1 (PHACTR1) gene, or fragments thereof, either alone or in combination with the level of expression of secreted integrin-binding phosphoprotein (SPP1), preferentially expressed antigen in melanoma (PRAME), growth differentiation factor 15 (GDF 15), and chemokine C-X-C motif ligand 10 (CXCL10) genes. Further, the invention relates to a diagnostic kit, comprising at least one substance for detection of the expression of PHACTR1, or fragments thereof, either alone or in combination with the detection of SPP1, PRAME, GDF15, and CXCL10, for the diagnosis or prognosis of melanoma.

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Номер записи: 52
29-03-2012 дата публикации

Methods of predicting complication and surgery in crohn's disease

Номер: US20120073585A1
Автор: Jerome I. Rotter, Kent D. Taylor, Marla Dubinsky, Stephan R. Targan
Принадлежит: Cedars Sinai Medical Center

The present invention relates to prognosing, diagnosing and treating an aggressive form of Crohn's disease characterized by rapid progression to complication and/or surgery from the time of diagnosis. In one embodiment, the prognosis, diagnosis and treatment is based upon the presence of one or more genetic risk factors.

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Номер записи: 53
29-03-2012 дата публикации

Method for Detecting and Quantitating Multiple-Subcellular Components

Номер: US20120075453A1
Автор: Michael Kilpatrick, Triantafyllos Tafas
Принадлежит: Ikonisys Inc

A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins.

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Номер записи: 54
29-03-2012 дата публикации

Kit and method for predicting cytarabine sensitivy of patient having acute myeloid leukemia

Номер: US20120077683A1
Автор: Dae-Soon Son, Dong-Hwan Kim, In-suk SOHN, Jhin-gook KIM, Jong-Suk Chung, Kyu-Sang Lee, Kyung-hee Park, Sin-ho JUNG, Sung-Min Chi, Sung-Ouk Jung, Won-Seok Chung
Принадлежит: SAMSUNG ELECTRONICS CO LTD

A kit and method for predicting cytarabine sensitivity of patients having acute myeloid leukemia are disclosed.

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Номер записи: 55
29-03-2012 дата публикации

Prognostic and predictive gene signature for non-small cell lung cancer and adjuvant chemotherapy

Номер: US20120077687A1
Автор: Christine Buerki, Fadia Saad, Joel Parker, Jonathan C. Schisler
Принадлежит: Individual

The application provides methods of prognosing and classifying lung cancer patients into poor survival groups or good survival groups and for determining the benefit of adjuvant chemotherapy by way of a multigene signature. The application also includes kits and computer products for use in the methods of the application.

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Номер записи: 56
05-04-2012 дата публикации

Expression Levels of COL4A1 and other Markers Correlating with Progression or Non-Progression of Bladder Cancer

Номер: US20120082994A1
Автор: Lars Dyrskjot Andersen, Torben Falck Orntoft
Принадлежит: Individual

Disclosed is determining expression levels protective or harmful markers for bladder cancer prognosis; particularly, determining the expression levels of COL4A3BP alone or in combination with the expression levels of MBNL2, FABP4, and NEK1 or other markers where increased expression levels of these protective markers relative to a control correlates with lack of bladder cancer progression and decreased expression levels correlates with bladder cancer progression or death. Also disclosed is determining the expression levels of COL4A1 alone or in any combination with the expression levels of UBE2C, BIRC5, COL18A1, KPNA2, MSN, ACTA2, and CDC25B or other markers where increased expression levels of these harmful markers relative to a control correlates with bladder cancer progression or death and decreased expression levels correlates with lack of bladder cancer progression Also disclosed are signatures of protective and harmful markers to predict likelihood of bladder cancer progression or non-progression.

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Номер записи: 57
12-04-2012 дата публикации

miRNAS AS THERAPEUTIC TARGETS IN CANCER

Номер: US20120087992A1
Автор: Bo Song, Jingfang Ju, Yuan Wang
Принадлежит: Research Foundation of State University of New York

Methods for modulating expression of a component of a cell, comprising contacting the cell with a nucleic acid comprising an miR-140 nucleic acid sequence in an amount sufficient to modulate the cellular component are provided. Overexpression of miR-140 inhibits cell proliferation in both U-2 OS (wt-p53) and HCT 116 (wt-p53) cell lines. Cells transfected with miR-140 are more resistant to chemotherapeutic agent methotrexate, mi-140 expression is related to HDAC4 protein expression. The claimed methods reduce the protein expression level of HDAC4 without degrading the target mRNA.

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Номер записи: 58
12-04-2012 дата публикации

Method for detection of genetically modified maize bt11

Номер: US20120088243A1
Автор: Qingkuo Lan, XIN ZHAO, YI Cheng, Yong Wang, Zhu Zhu
Принадлежит: Central Laboratory of Tianjin Academy of Agricultural Sciences

The invention discloses a method for detection of genetically modified maize BT11. The principle of the method is that the DNA template of the sample is amplified at a temperature of 63° C.˜65° C. for 45˜60 min by using 4 specific primers and a DNA polymerase with strand displacement activity. The identification thereof is to make a judgment on whether BT11 component is contained in the sample by directly observing the turbidity in the reaction tube or the color change after the addition of SYBR Green with naked eyes or by agarose gel electrophoresis. The detection method of the invention has the advantages of high specificity, quickness, simplicity and convenience and the like, which provides a convenient method for detection of genetically modified maize BT11 with an extensive application prospect.

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Номер записи: 59
12-04-2012 дата публикации

Composition and methods for rapid detection of hiv by loop-mediated isothermal amplification (lamp)

Номер: US20120088244A1
Автор: Donna Rudolph, Kelly Curtis, S. Michele Owen
Принадлежит: US Department of Health and Human Services

Methods and compositions for detection of HIV nucleic acids in a sample, such as a biological sample obtained from a human subject, are provided according to embodiments of the present invention which include providing a reaction mixture including at least one LAMP, accelerated LAMP, RT-LAMP or RT-accelerated LAMP assay primer set specific for HIV-I or HIV-2 nucleic acids and the biological sample to be tested for presence of HIV-I and/or HIV-2 nucleic acids; incubating the reaction mixture under conditions suitable to produce a LAMP assay reaction product; and detecting the reaction product. Primers and primer sets for use in LAMP assays of HIV-I or HIV-2 nucleic acids are provided.

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Номер записи: 60
12-04-2012 дата публикации

Methods of diagnosing insulin resistance and sensitivity

Номер: US20120088245A1
Автор: Anny Xiang, Jerome I. Rotter, Kent D. Taylor, Leslie Raffel, Mark O. Goodarzi, Thomas A. Buchanan, Xiuqing Guo, Yii-Der I. Chen
Принадлежит: Cedars Sinai Medical Center

Methods of diagnosing susceptibility to metabolic insulin resistance and other related conditions are disclosed. The method provides means of diagnosing susceptibility to insulin resistance in Hispanic Americans by determining the presence of a risk haplotype at the LPL locus, the LPIN1 locus, and/or elevated levels of gamma-glutamyl transferase.

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Номер записи: 61
03-05-2012 дата публикации

Abbreviated hcv therapy for hcv infected patients with il28b c/c genotype

Номер: US20120107278A1
Автор: Miriam Michelle Berrey, Peter Schaefer Price, JR., William T. Symonds
Принадлежит: Pharmasset Inc

Disclosed herein is a method for administering an abbreviated hepatitis C virus (HCV) treatment regimen for an HCV-infected, rs12979860 single nucleotide polymorphism (SNP) (C/C) patient, which comprises: administering to said patient an effective amount of each of a direct-acting antiviral, pegylated interferon alfa-2a, and ribavirin; wherein the abbreviated HCV treatment regimen is less than 48 weeks. Also disclosed herein is a method of detecting an rs12979860 single nucleotide polymorphism (SNP) in a hepatitis C virus (HCV) infected patient comprising: detecting the rs12979860 SNP of chromosome 19 in a biological sample from a patient, wherein an rs12979860 SNP C/C patient is administered an abbreviated treatment regimen with a direct-acting antiviral agent in combination with peginterferon alfa-2a and ribavirin; and wherein the abbreviated HCV treatment regimen is less than 48 weeks.

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Номер записи: 62
03-05-2012 дата публикации

Methods and compositions for assessing patients with reproductive failure using immune cell-derived microrna

Номер: US20120107825A1
Автор: Edward E. Winger, Jane L. REED
Принадлежит: Reed Jane L, Winger Edward E

The invention is directed to methods and compositions for collecting immune cells, preferably peripheral blood mononuclear cells (PBMCs), before or after an intervention, extracting microRNA-comprising RNA from said cells, quantifying microRNAs within the extracted RNA, determining one or more microRNAs that display a bimodal response amongst a statistically sufficient number of patient samples. Patients are then preferably segregated into groups according to their response.

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Номер записи: 63
03-05-2012 дата публикации

ZNF217 A New Prognostic And Predictive Biomarker Of Recurrent Invasive And Metastatic Phenotypes In Breast Cancer

Номер: US20120107826A1
Автор: Julie Vendrell, Pascale Cohen, Pierre Roux
Принадлежит: CENTRE LEON BERARD

The present invention relates to methods for determining the prognosis of a cancer. The methods involve determining the level of expression of the ZNF217 gene in a cancer cell sample or in a tumor sample wherein over-expression of ZNF217 is correlated with likelihood of metastasis and with likelihood of relapse/recurrence of the cancer.

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Номер записи: 64
10-05-2012 дата публикации

Assay for detecting and quantifying hiv-1

Номер: US20120115125A1
Автор: Astrid R.W. Schroder, Daniel P. Kolk, Glenn J. Sawyer
Принадлежит: Gen Probe Inc

Method of detecting HIV-1 nucleic acids using nucleic acid amplification and a molecular torch hybridization probe. The invented method is characterized by high levels of precision in the quantitation of HIV-1 targets at low copy numbers, and by accurate detection of different HIV-1 subtypes, including M group and O group variants.

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Номер записи: 65
10-05-2012 дата публикации

Serotonin transporter gene and treatment of alcoholism

Номер: US20120115149A1
Автор: Bankole A. Johnson
Принадлежит: UNIVERSITY OF VIRGINIA PATENT FOUNDATION

The gene (SLC6A4) responsible for encoding the serotonin transporter (SERT) has a functional polymorphism at the 5′-regulatory promoter region, which results in two forms, long (L) and short (S). The LL-genotype is hypothesized to play a key role in the early onset of alcohol use. The present invention discloses the differences in treatment and diagnosis based on the L or short genotypes as well as on a single nucleotide polymorphism of the SERT gene, the 3′ UTR SNP rs1042173. The present invention demonstrates the efficacy of using the drug ondansetron and similar drugs for treatment based on diagnosing variations in the polymorphisms of the SERT gene and expression and activity of the SERT gene, as well as methods for diagnosing susceptibility to abuse of alcohol and other addiction-related diseases and disorders, for monitoring treatment and/or abuse (addictive behavior), and for determining which treatment should be used.

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Номер записи: 66
10-05-2012 дата публикации

Methods for Screening for Antibiotic Compounds

Номер: US20120115157A1
Автор: Ambrose Cheung, Zhibiao Fu
Принадлежит: Dartmouth College

The present invention is a method for screening drugs for antibiotic activity by screening a drug for activity to disrupt a toxin-antitoxin complex in a bacterial cell.

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Номер записи: 67
10-05-2012 дата публикации

Compositions and methods for classifying thyroid nodule disease

Номер: US20120115743A1
Автор: Elai Davicioni, Sam Michael Wiseman
Принадлежит: Individual

A system for classifying thyroid nodule tissue as malignant or benign is provided that is based on the identification of sets of gene transcripts, which are characterized in that changes in expression of each gene transcript within a set of gene transcripts can be correlated to with either malignant or benign thyroid nodule disease. The thyroid classification system provides for sets of “thyroid classifying” target sequences and further provides for combinations of polynucleotide probes and primers derived there from. These combinations of polynucleotide probes can be provided in solution or as an array. The combination of probes and the arrays can be used for diagnosis. The invention further provides further methods of classifying thyroid nodule tissue.

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Номер записи: 68
10-05-2012 дата публикации

Plant resistant to a pathogen

Номер: US20120117683A1
Автор: Michel De Lange, Olaf Zonneveld, Victor Segura, William Briggs
Принадлежит: SYNGENTA PARTICIPATIONS AG

The present invention relates to novel Lactuca sativa plants resistant to Bremia , and to seeds of said plants. The present invention also relates to methods of making such plants and their seeds. The invention further relates to markers and the use thereof in marker assisted breeding and for identifying the Bremia resistance trait.

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Номер записи: 69
17-05-2012 дата публикации

Method for determining predisposition to pulmonary infection

Номер: US20120121593A1
Автор: Christoph Lange, Craig Gerard, Gerald Pier, Hara Levy, Scott Weiss
Принадлежит: Individual

Provided herein are methods and materials for diagnosing a subject's predisposition for pulmonary infection in a CF subject by detecting a pulmonary infection genetic marker. Pulmonary infection markers have been identified in the IL-1 gene cluster and may be useful in predicting CF disease progression and assessing a CF subject's response to therapy.

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Номер записи: 70
17-05-2012 дата публикации

Compositions for use in identification of bacteria

Номер: US20120122099A1
Автор: David J. Ecker, Lawrence Blyn, Rangarajan Sampath, Thomas A. Hall
Принадлежит: Ibis Biosciences Inc

The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.

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Номер записи: 71
17-05-2012 дата публикации

Compositions for use in identification of bacteria

Номер: US20120122102A1
Автор: David J. Ecker, Lawrence Blyn, Rangarajan Sampath, Thomas A. Hall
Принадлежит: Ibis Biosciences Inc

The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.

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Номер записи: 72
17-05-2012 дата публикации

Real time cleavage assay

Номер: US20120122105A1
Автор: Graham P. Lidgard, Hatim Allawi, Hongzhi Zou, Michael J. Domanico, Rebecca Oldham-Haltom
Принадлежит: Individual

A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

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Номер записи: 73
17-05-2012 дата публикации

Markers for endometrial cancer

Номер: US20120122726A1
Автор: Andreas Döll, Antonio Gil Moreno, Cristina Perez, Elisabet Rossell, Jaume REVENTÓS PUIGJANER, Miguel Abal Posada, Tamara Maes
Принадлежит: Geadic Biotec AIE

The invention relates to the surprising finding that biomarkers corresponding to ACAA1, AP1M2, CGN, DDR1, EPS8L2, FASTKD1, GMIP, IKBKE, P2RX4, P4HB, PHKG2, PPFIBP2, PPP1 R16A, RASSF7, RNF183, SIRT6, TJP3, EFEMP2, S0CS2, and DCN are differentially expressed in control samples as compared to samples from patients having endometrial cancer and are therefore useful for detecting endometrial cancer. In particular these biomarkers having excellent sensitivity, specificity, and/or the ability to separate affected from non affected individuals. Furthermore, the inventors found that the differential expression of these biomarkers in primary endometrial cancer tumor tissue is correlated to their expression level in uterine fluid samples as compared to control values. Thus these biomarkers are robust in that they are found to be differentially expressed in several different types of samples from affected individuals.

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Номер записи: 74
17-05-2012 дата публикации

Bcl Gene Primers for Detection and Fingerprinting of Bacillus anthracis

Номер: US20120123108A1
Автор: Slawomir Lukomski
Принадлежит: WEST VIRGINIA UNIVERSITY

B. anthracis detection primers can be used to amplify the conserved regions of bclB alleles encoding the collagen-like proteins found in B. anthracis , as opposed to other Bacillus species, during PCR amplification of extracted Bacillus DNA or Bacillus spores. Additionally, B. anthracis strain fingerprinting primers amplify bclA-E polymorphic regions of collagen-like proteins found in B. anthracis strains. The B. anthracis strains differ in basepair size of these polymorphic coding regions of bclA-E so that strains can be discriminated based upon distinct PCR-band patterns that migrate differently in size while resolved on an agarose gel. DRAWINGS

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Номер записи: 75
17-05-2012 дата публикации

Methods for assessing responsiveness of b-cell lymphoma to treatment with anti-cd40 antibodies

Номер: US20120123695A1
Автор: Bart Burington, David Dornan
Принадлежит: Genentech Inc

The invention provides methods and kits useful for predicting or assessing responsiveness of a patient having B-cell lymphoma to treatment with anti-CD40 antibodies.

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Номер записи: 76
24-05-2012 дата публикации

Truncated cd20 protein, deltacd20

Номер: US20120129167A1
Автор: Carole Henry, Christophe Borg, Christophe Ferrand, Marina Deschamps, Pierre Tiberghien, Pierre-Simon Rohrlich
Принадлежит: Francais du Sang Ets

The present invention relates in particular to a protein from an alternative splicing of the gene encoding CD20, the nucleic acid sequences encoding the protein according to the invention, a mutated form of the CD20 gene as well as drugs, diagnostic tools, diagnostic methods and treatment methods using the protein and the nucleic acid sequences according to the invention.

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Номер записи: 77
24-05-2012 дата публикации

Method for renal disease diagnosis and prognosis using annexin a1 and rab23 as markers

Номер: US20120129175A1
Автор: Ann Chen, Hao-Ai Shui, Shuk-Man Ka
Принадлежит: NATIONAL DEFENSE MEDICAL CENTER

Use of Annexin A1 or Rab23 as a biomarker for diagnosing kidney disease or assessing efficacy of kidney disease treatment.

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Номер записи: 78
24-05-2012 дата публикации

Cytokines as prognostic markers of respiratory-tract infection following major surgery

Номер: US20120129176A1
Автор: Dermot Kelleher, Mary White, Owen Ross McManus, Patrick Stordeur, Thomas Ryan, Vincent Young
Принадлежит: College of the Holy and Undivided Trinity of Queen Elizabeth near Dublin

The invention relates to the use of a certain subset of cytokine markers as prognostic variables of infection status in an individual, and especially as prognostic markers of a patients developing severe infection such as pneumonia, and respiratory tract infection following surgery. The subset of cytokine markers consists of the interleukin cytokines IL-2, IL-7, IL-23, IL-27, and IL-IO, and Interferon-γ (INFγ) and Tissue Necrosis Factor-α (TNFα). The markers may be employed as individual prognostic variables of infection status, or they may be used in pairs or other combinations. Generally, the abundance of the markers is correlated with infection status by means of an absolute pre-operative value of biomarker abundance, ratio's of pre-operative to post-operative biomarker abundance, or ratio values for pairs of certain biomarkers within the subset. Typically, cytokine abundance is expressed in terms of mRNA copy number wherein the copy numbers are ideally normalised to a house keeping gene and quantification of mRNA copy number is determined by RT-PCR containing reference serial dilutions of cytokine specific cDNA.

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Номер записи: 79
24-05-2012 дата публикации

Scn5a splicing factors and splice variants for use in diagnostic and prognostic methods

Номер: US20120129179A1
Автор: Samuel Dudley
Принадлежит: University of Illinois

Provided herein are methods of identifying a subject at risk for arrhythmias, heart failure or sudden cardiac death. In exemplary aspects, the method comprises the step of determining a level of splicing factor hLuc7a, splicing factor RBM25 and/or PERK in a biological sample obtained from the subject, wherein an increased level, compared to a control level, indicates a risk for arrhythmia or heart failure. The invention also provides methods of diagnosing hypertrophic cardiomyopathy (HCM), or a risk therefor, in a subject. The method comprises the step of determining a level of E28D, RBM25, PRPF40A, or LUC7L3, or a combination thereof, in a biological sample obtained from the subject, wherein an increased level is indicative of the subject having HCM or a risk therefor. Diagnostic kits comprising binding agents for the markers are furthermore provided.

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Номер записи: 80
24-05-2012 дата публикации

Marker and reagent for detection of human il-17-producing helper t cells, and method for detection of human il-17-producing helper t cells

Номер: US20120129724A1
Автор: Hirokazu Kurata, Hitoshi Uga, Masafumi Ikeda, Masakazu Kadowaki, Masatoshi Yanagida, SATOSHI Tanaka, Takahiro Okazawa, Yoshiaki Miyamoto
Принадлежит: Sysmex Corp

The present invention relates to a marker allowing specific detection of human IL-17-producing helper T-cells (human Th17 cells), a method for specifically detecting human Th17 cells and a reagent for detecting human Th17 cells.

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Номер записи: 81
31-05-2012 дата публикации

Treatment of 'c terminus of hsp70-interacting protein' (chip) related diseases by inhibition of natural antisense transcript to chip

Номер: US20120135941A1
Автор: Carlos Coito, Joseph Collard, Olga Khorkova Sherman
Принадлежит: Curna Inc

The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of ‘C terminus of HSP70-Interacting Protein’ (CHIP), in particular, by targeting natural antisense polynucleotides of ‘C terminus of HSP70-Interacting Protein’ (CHIP). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of CHIP.

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Номер записи: 82
31-05-2012 дата публикации

Tomato hybrid ps 01565172

Номер: US20120137381A1
Автор: Douglas Heath
Принадлежит: Individual

The invention provides seed and plants of tomato hybrid PS 01565172 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of tomato hybrid PS 01565172 and the parent lines thereof, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

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Номер записи: 83
31-05-2012 дата публикации

Cucumber Vein Yellowing Virus (CVYV) Resistant Cucumber Plants (Cucumis Sativus L.)

Номер: US20120137388A1
Автор: Brigit Van Kampen, Jaap Mazereeuw, Nanne Faber, Ronald Wilterdink
Принадлежит: Enza Zaden de Enkhuizer Zaadhandel BV

Molecular markers genetically linked to, and capable of identifying, a genetic locus in the cucumber plant ( Cucumis sativus L.) genome conferring a general resistance against tobamoviruses, and especially against two commercially important pathogenic tobamoviruses, i.e., cucumber green mottle mosaic virus (CGMMV) and cucumber fruit mottle mosaic virus (CFMMV) are provided. Methods for providing a cucumber plant ( Cucumis sativus L.,) plants, plant parts and fruits with resistance against tobamoviruses and especially against two commercially important pathogenic tobamoviruses, i.e., cucumber green mottle mosaic virus (CGMMV) and cucumber fruit mottle mosaic virus (CFMMV) are also provided.

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Номер записи: 84
07-06-2012 дата публикации

Use of pkc isozymes for diagnosis and treatment of neuroblastoma

Номер: US20120141498A1
Автор: Mildred E. Acevedo-Duncan, Rekha S. Patel
Принадлежит: UNIVERSITY OF SOUTH FLORIDA, US Department of Veterans Affairs VA

The present invention pertains to the use of PKC-epsilon (PKC-ε), PKC-delta (PKC-δ), PKC-eta, and/or PKC-theta as biomarker(s) for prediction and/or detection of neuroblastoma, as well as therapeutic targets for treatment of neuroblastoma.

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Номер записи: 85
07-06-2012 дата публикации

Trichomonas vaginalis testing using tv5.8s as a target

Номер: US20120142003A1
Автор: Cori Anne Barfield, Kathryn Watts Weaver, Mitra Choudhury Singhal
Принадлежит: PATH

The invention provides methods, reagents and kits for determining the presence of Trichomonas vaginalis in a test sample.

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Номер записи: 86
07-06-2012 дата публикации

Diagnostic transcriptomic biomarkers in inflammatory cardiomyopathies

Номер: US20120142544A1
Автор: Bettina Heidecker, Joshua M. Hare
Принадлежит: UNIVERSITY OF MIAMI

Molecular signatures that function as very sensitive diagnostic biomarker for myocarditis, heart disease and disorders thereof, are identified.

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Номер записи: 87
07-06-2012 дата публикации

Diagnosis and treatment of adrenocortical tumors using human microrna-483

Номер: US20120142753A1
Автор: Electron Kebebew, Erin Patterson
Принадлежит: National Institutes of Health NIH, US Department of Health and Human Services

Disclosed herein are methods of diagnosing and treating a malignant adrenocortical tumor, including adrenocortical carcinoma. In some examples, methods of diagnosing a malignant adrenocortical tumor include detecting expression of at least one microRNA (miR) gene product, such as miR-100, miR-125b, miR-195, miR-483-3p, miR-483-5p and IGF2 mRNA in a sample obtained from the subject with an adrenocortical tumor and comparing expression of at least one of these miR gene products and IGF2 mRNA in the sample obtained from the subject to a control. Altered expression of at least one of the miR gene products and IGF2 mRNA, such as a decrease in miR-100, miR-125b or miR-195 or an increase in miR-483-3p, miR-483-5p, and an increase in IGF2 mRNA, in the sample obtained from the subject compared to the control indicates a malignant adrenocortical tumor.

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Номер записи: 88
07-06-2012 дата публикации

Caspase-8 and skin disease

Номер: US20120144504A1
Автор: Andrei Kovalenko, David Wallach, Jin Chul Kim, Tae-Bong Kang
Принадлежит: Yeda Research and Development Co Ltd

The invention relates to the treatment or prevention of an inflammatory skin disease, disorder or condition, by modulating a protein that is normally regulated by caspase-8 in the skin or by increasing caspase-8 activity or level in the skin. Another aspect of the invention relates to methods for diagnosing an inflammatory skin disease, disorder or condition or a predisposition to develop said disease disorder or condition in an individual. Further aspects of the invention relate to methods for identifying target proteins involved in the course or pathology of an inflammatory skin disease, disorder or condition and to methods of screening a candidate compound for treating said disease, disorder or condition. In particular, the invention relates to inflammatory skin diseases such as atopic dermatitis and psoriasis.

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Номер записи: 89
14-06-2012 дата публикации

Genetic Variants in the TCF7L2 Gene as Diagnostic Markers for Risk of Type 2 Diabetes Mellitus

Номер: US20120149016A1
Автор: Struan F.A. Grant
Принадлежит: DECODE GENETICS EHF

Polymorphisms in the gene TCF7L2 are shown by association analysis to be a susceptibility gene for type II diabetes. Methods of diagnosis of susceptibility to diabetes, of decreased susceptibility to diabetes and protection against diabetes, are described, as are methods of treatment for type II diabetes.

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Номер записи: 90
21-06-2012 дата публикации

Composition for diagnosis of liver metastasis of colorectal cancer and the use thereof

Номер: US20120156681A1
Автор: Ho-Kyung Chun, Woo Yong Lee, Yong Beom Cho
Принадлежит: SAMSUNG LIFE PUBLIC WELFARE FOUNDATION

The present invention relates to a composition for diagnosis of liver metastasis of colorectal cancer and the use thereof, and more particularly to a composition for diagnosis of liver metastasis of colorectal cancer, which comprises either a substance for measuring the mRNA level of CCL7 (Chemokine (C-C motif) ligand 7) gene or a substance for measuring the level of a protein which is encoded by the gene. According to the present invention, whether liver metastasis of colorectal cancer occurred can be diagnosed by measuring the mRNA expression level of the CCL7 gene or the expression level of the CCL7 protein, and the use of the composition comprising an inhibitor of CCL7 gene allows the treatment of colorectal cancer or the liver metastasis of colorectal cancer.

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Номер записи: 91
21-06-2012 дата публикации

Method for detecting and quantifying rare mutations/polymorphisms

Номер: US20120156685A1
Автор: Charles R Cantor, Chunming Ding
Принадлежит: Boston University

The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).

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Номер записи: 92
21-06-2012 дата публикации

miRNA FINGERPRINT IN THE DIAGNOSIS OF MULTIPLE SCLEROSIS

Номер: US20120157337A1
Автор: Andreas Keller, Anne Borries, Eckart Meese, Markus Beier, Peer Friedrich Staehler
Принадлежит: FEBIT HOLDING GMBH

The present invention provides novel methods for diagnosing diseases based on the determination of specific miR-NAs that have altered expression levels in disease states compared to healthy controls.

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Номер записи: 93
21-06-2012 дата публикации

Identification of Inhibitors of a Bacterial Stress Response

Номер: US20120157375A1
Автор: Kenneth Charles Keiler, Sarah Ellen Ades
Принадлежит: PENN STATE RESEARCH FOUNDATION

A system, a composition, a method and a kit for identifying anti-bacterial agents are provided. The invention described herein is useful in identifying inhibitors of any bacterial stress response. Moreover, the invention can be applied to any sRNA and its target, any transcription factor and its target, and any transcription factor/sRNA pair (i.e., a transcription factor that regulates a sRNA). In particular, the present invention provides a system, a composition, a method and a kit for the identification of cyclic peptides that block the σ E pathway in Escherichia coli.

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Номер записи: 94
21-06-2012 дата публикации

Extracellular vesicles derived from gram-positive bacteria, and use thereof

Номер: US20120159658A1
Автор: Eun Young Lee, Ji Hyun Kim, Seng Jin Choi, Sung Wook Hong, Yong Song Gho, Yoon Keun Kim
Принадлежит: AEON MEDIX Inc

The present application relates to extracellular vesicles (EVs) derived from gram-positive bacteria. In detail, the present application provides animal models of disease using extracellular vesicles derived from gram-positive bacteria, provides a method for screening an active candidate substance which is capable of preventing or treating diseases through the animal models of disease, provides vaccines for preventing or treating diseases caused by extracellular vesicles derived from gram-positive bacteria, and provides a method for diagnosing the causative factors of diseases caused by gram-positive bacteria using extracellular vesicles.

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Номер записи: 95
28-06-2012 дата публикации

Digital Quantification of DNA Methylation

Номер: US20120164638A1
Автор: Bert Vogelstein, Kenneth W. Kinzler, Luis Diaz, MENG Li, Nickolas Papadopoulos, Sanford Markowitz
Принадлежит: CASE WESTERN RESERVE UNIVERSITY, JOHNS HOPKINS UNIVERSITY

Abnormal DNA methylation can be used as a biomarker in cancer patients. For such purposes, it is important to determine precisely the fraction of methylated molecules in an analyzed sample. A technology we term Methyl-BEAMing achieves this goal. Individual bisulfite-treated DNA molecules can be PCR-amplified within aqueous nanocompartments containing beads, resulting in a population of beads each containing thousands of copies of the template molecule. After hybridization with probes specific for methylated sequences, the beads can be analyzed by flow cytometry. This approach enables detection and enumeration of one methylated molecule in a population of ˜5000 unmethylated molecules. Methyl-BEAMing provides digital quantification of rare methylation events and is generally applicable to the assessment of methylated genes in clinical samples.

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Номер записи: 96
28-06-2012 дата публикации

Simultaneous determination of aneuploidy and fetal fraction

Номер: US20120165203A1
Автор: David A. COMSTOCK, Gabrielle HEILEK, Manjula CHINNAPPA, Richard P. Rava, Stephen Quake
Принадлежит: Verinata Health Inc

The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained form a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.

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Номер записи: 97
05-07-2012 дата публикации

Further use of protein kinase n beta

Номер: US20120171220A1
Автор: Anke Klippel-Giese, Jörg Kaufmann
Принадлежит: SILENCE THERAPEUTICS AG

The present invention is related to use of protein kinase N beta or a fragment or derivative thereof as a downstream target of the PI 3-kinase pathway, preferably as a downstream drug target of the PI 3-kinase pathway.

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Номер записи: 98
05-07-2012 дата публикации

Gene Expression Markers for Colorectal Cancer Prognosis

Номер: US20120171688A1
Автор: Drew Watson, James Hackett, Joffre B. Baker, Kim Clark-Langane, Soonmyung Palk, Wayne Cowens
Принадлежит: Genomic Health Inc, NSABP Foundation Inc

A method of predicting clinical outcome in a subject diagnosed with colorectal cancer comprising determining evidence of the expression of one or more predictive RNA transcripts or their expression products in a biological sample of cancer cells obtained from the subject.

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Номер записи: 99
05-07-2012 дата публикации

Biomarkers and uses thereof in prognosis and treatment strategies for right-side colon cancer disease and left-side colon cancer disease

Номер: US20120172244A1
Автор: Amanda Hummon, Steven Buechler
Принадлежит: University of Notre Dame

Genetic biomarkers for left side colon cancer (LCC) (such as expression levels of an RNA transcript or expression product of NOX4, MMP3, or a combination) and right side colon cancer (RCC) (such as expression levels of an RNA transcript or expression product of CDCX2, FAM69A, or a combination), are disclosed. Methods for using the biomarkers in providing a prognosis of relapse-free survival probability in patients having LCC or RCC are also presented. Prognostic panels using gene expression values of the biomarkers are also presented. Computer implemented methods employing the biomarkers, and as well as for determining relapse-free survival probability in a patient having RCC or LCC are provided. A genetic method for classifying a colon cancer tissue as a RCC or as a LCC is also disclosed.

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Номер записи: 100