VERFAHREN UND REAGENS ZUR BESTIMMUNG VON ALPHA- -AMYLASE

PROOF OF IONS IN LIQUIDS

PROCEDURE FOR THE REGULATION OF ALKALINE PHOSPHATASE UNDER REMOVAL OF HEMOGLOBIN DISTURBANCES

PROCEDURE AND REAGENT FOR THE REGULATION OF ALPHA - AMYLASE

PROCEDURE FOR ALPHA AMYLASEBESTIMMUNG AND TEST SET.

REGULATION OF POTASSIUM IONS IN LIQUIDS.

BY AROMATICS SUBSTITUTED GLYKOSID

SUBSTRATES FOR BETA-GALACTOSIDASE

ABSTRACT OF THE DISCLOSURE A substrate for B-galactosldase having the general formula

BIOLOGICAL EXTRACTS AND METHOD FOR MAKING SAME

Endotoxin clottable extracts are standardized by inactivating endogenous inhibitor, determining the coagulase activity of the extract and proportioning the extract into an endotoxin assay reaction mixture so as to provide coagulase activity at a predetermined level. In most circumstances the coagulogen concentration is also determined and, if the concentration is lower than a predetermined amount, coagulogen is added to the extract. Inhibitor is advantageously inactivated by adjusting the extract pH to a strongly alkaline level, incubating and readjusting the pH to near neutrality.

IDENTIFICATION OF LIGANDS BY SELECTIVE AMPLIFICATION OF CELLS TRANSFECTED WITH RECEPTORS

A method of detecting a substance capable of acting as a ligand, the method comprising: (a) incubating, under conditions permitting cell amplification, cells transfected with DNA coding for a receptor capable of influencing cell amplification in response to a ligand, the cells comprising a marker of cell amplification, with a test substance which is a potential agonistor antagonist of the receptor, and (b) after a period of time sufficient to permit cell amplification determining the presence or absence of amplification of cells containing the marker relative to cells not containing the marker.

Reagens zur Bestimmung von alkalischer oder saurer Phosphatase

Verfahren und Mittel zur Bestimmung des Gehalts an alkalischer Phosphatase im Serum

PROCEDURE FOR THE DETERMINATION OF THE AMYLASEGEHALTS FROM SAMPLES AND MEANS TO THE EXECUTION OF THE PROCEDURE.

METHOD FOR DETERMINING THE AMYLASE.

STABILIZED LIQUID DIAGNOSTIC COMPOSITION CONTAINING A PHOSPHATE AND METHOD FOR PREPARATION THEREOF.

Process for the preparation of a substrate for the determination of alpha-amylase

PROCEDURE AND TEST MIXTURE FOR THE REGULATION OF ALPHA AMYLASE.

Procedure for the determination of the alkaline Phosphatase.

ASSAY REAGENTS

REAGENT FOR THE DETERMINATION OF ALKALINE PHOSPHATASE IN SERUM

Hydrolytic enzyme inhibitors/inactivators and methods for using same

The present invention provides compounds that function as hydrolytic enzyme inhibitors (inactivators) and substrates. These compounds are useful in assays to detect and measure levels of hydrolytic enzyme activity and are more particularly useful in treatment regimens for various disease states and conditions implicating the underlying specific hydrolytic enzyme. Examples of hydrolytic enzymes include, but are not limited to, phospholipases, lipases, esterases, proteases, etc.

An alpha-amylase assay method and reagent kit

An -amylase assay method is described together with a reagent kit for use therein. An oligosaccharide substrate for -amylase is provided. The substrate contains at least 3 glucose units. Its reducing-end glucose unit is bonded, via a bond cleavable by - or ß- glucosidase, to a label which exhibits an optically measurable change upon cleavage of the bond. Its terminal glucose unit is bonded to a blocking substituent which inhibits cleavage by exo-enzymes of the bond between the terminal glucose unit and the adjacent glucose unit. A liquid assay sample is contacted with the oligosaccharide substrate and with an exo-enzyme capable of cleaving the bond between the reducing-end glucose unit and the label. The optically measurable change is used as a measure of -amylase in the sample.

Method for measuring ions in liquid and composition therefor

Verfahren und Zusammensetzung zum Nachweis von Natrium-Ionen in Flüssigkeiten

DRY ANALYTICAL ELEMENT CONTAINING SELF-DEVELOPING SUBSTRATE FOR USE IN ANALYSIS OF LIQUID

MALTOHEPTAOSIDE DERIVATIVES

Improvements in and relating to booking or stripping and booking machines for tobacco leaves

... 459,241. Stripping and booking tobacco. INTERNATIONAL CIGAR MACHINERY CO., 5520, 2nd Avenue, Brooklyn, New York, U.S.A. July 5, 1935, No. 19225. Convention date, Feb. 27. [Class 130] In a machine for booking, or stripping and booking, tobacco leaves, including a rotary booking drum and means such as endless travelling belts for holding the leaves in book formation on the surface of the drum, a pneumatic device is provided for effecting an automatic spreading or opening of the leaves by the flow of air over their surfaces as they are fed or guided to the surface of the drum. As shown, the leaves are held on the booking drum 35 by belts 34 travelling on rollers 24, 36, tension rollers 37, and rollers 39, 29. The tension rollers 37 are carried by arms 38 on a shaft 19 provided with weighted arms 40. The rollers 24 are supported by arms 22 on a shaft 16 arranged to be swung by a handle 21 into the position shown in dotted lines in Fig. 1 ; the belts 34 then pass over a rod 25 carried by the ...

AMYLASE DETERMINATION

AN A AMYLASE ASSAY METHOD AND REAGENT KIT

Method of measuring the amount of alpha -amylase in a liquid sample, including the steps of providing an oligosaccharide substrate for alpha -amylase, the substrate being characterized in that it contains at least 3 glucose units, its reducing-end glucose unit is bonded, via a bond cleavable by alpha - or beta -glucosidase, to a label which exhibits an optically measurable change upon cleavage of the bond, and its terminal glucose unit is bonded to a blocking substituent which inhibits cleavage by exo-enzymes of the bond between the terminal glucose unit and the adjacent glucose unit; contacting the sample with the oligosaccharide substrate and with a first exo-enzyme capable of cleaving the bond between the reducing-end glucose unit and the label, and measuring the optically measurable change as a measure of alpha -amylase in the sample.

IDENTIFICATION OF LIGANDS BY SELECTIVE INCREASE OF, BY RECEPTORS CELLS TRANZFIZIERTEN

PROCEDURE AND COMPOSITION FOR THE PROOF OF CHLORIDE IONS IN LIQUIDS

TEST COMPOSITIONS TO ELECTROLYTENMESSUNG USING ALPHA AMYLASE

REAGENT FOR THE REGULATION OF ENZYMES.

WITH AROMATICS SUBSTITUTED GLYKOSID.

PROCEDURE AND REAGENT FOR THE REGULATION OF ALPHA - AMYLASE

OLIGOGLUCOSIDE DERIVATIVES

SUBSTRATES FOR BETA-GALACTOSIDASE

HETEROBIFUNCTIONAL ANTIBODIES POSSESSING DUAL CATALYTIC AND SPECIFIC ANTIGEN BINDING PROPERTIES AND METHODS USING THEM

ANALYTICAL ELEMENT AND METHOD FOR THEOPHYLLINE DETERMINATION USING BUFFER IN SPREADING ZONE

ANALYTICAL ELEMENT AND METHOD FOR THEOPHYLLINE DETERMINATION USING BUFFER IN SPREADING ZONE Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is carried out at a pH of 9 or less. The element comprises a porous spreading zone and at least one other zone in fluid contact with the spreading zone. The isoenzyme of alkaline phosphatase and a suitable substrate for the isoenzyme are located in different zones of the element. The element also contains a buffer for maintaining the pH at 9 or less, substantially all of which is located in the spreading zone.

METHOD FOR DETERMINING ALKALINE PHOSPHATASE AND ELIMINATING HAEMOGLOBIN DISTURBANCES

The invention concerns a method for the determination of alkaline phosphatase in a sample by optical measurement in which interference by free haemoglobin or blood substitutes is eliminated by means of certain wavelength combinations, a method for eliminating interference caused by free haemoglobin or blood substitutes in a determination of alkaline phosphatase and the use of certain wavelength combinations to eliminate interference by free haemoglobin or blood substitutes.

HYDROLYTIC ENZYME INHIBITORS AND SUBSTRATES AND ASSAYS, METHODS AND KITS EMBODYING SAME

... 2064793 9103544 PCTABS00003 The present invention provides novel hydrolytic enzyme inhibitors (inactivators) and substrates that have three functional parts: 1) a moiety that is specifically cleavable upon contact of the compound with a specific hydrolytic enzyme which moiety when cleaved is detectable and measurable, 2) a moiety that is recongnized by a specific hydrolytic enzyme and which interacts therewith and the substrate is specifically cleavable upon such interaction and 3) a linker moiety linking moieties 1) and 2). These novel compounds are useful in assays to detect and mesure levels of hydrolytic enzyme activity and are also useful in treatment regimens for various disease states and conditions implicating the underlying specific hydrolytic enzyme. Examples of hydrolytic enzymes include, but are not limited to, phopholipases, lipases, esterases, proteases, etc.

ASSAY REAGENTS

PROCESS AND NECESSARY Of TEST FOR PROPORTIONING OF AMYLASE OF the SERUM

L'invention se rapporte à l'analyse biologique. Elle concerne un procédé pour déterminer la teneur en amylase d'un échantillon, comprenant les étapes selon lesquelles : a. On ajoute à une solution contenant une quantité mesurée de l'échantillon un substrat polysaccharide défini ayant la formule suivante :

Detection of fertile period and pregnancy - with test kit contng. buffer and cpd. hydrolysable to p-nitrophenol etc.

COMPOSITION POUR L'ANALYSE DE LA PHOSPHATASE ALCALINE ET PROCEDE POUR SA MISE EN OEUVRE

Composition pour la détermination de l'activité de la phosphatase alcaline. La composition comprend un sel de l'acide p-nitrophényl-phosphorique, un sel de magnésium, la borate de sodium et le 2-amino-2-hydroxyméthyl-1,3-propanediol.

Process and reagent for the determination of alpha -amylase

A process and a reagent for the determination of alpha -amylase involve enzymatic splitting of an alpha -amylase substrate in the presence of alpha -glucosidase and beta -glucosidase and measurement of a fission product. As substrate, there is used a 4-nitrophenyl- beta ,D-maltoheptaoside electronegatively substituted in the phenyl nucleus.

Реактив для определения фосфатаз

VERFAHREN ZUR BESTIMMUNG VON ALKALISCHER PHOSPHATASE

VERFAHREN UND REAGENS ZUR BESTIMMUNG VON ALPHA-AMYL&SE

Mit Aromaten substituiertes Glykosid.

VERFAHREN ZUR BESTIMMUNG VON ENZYMATISCHER WIRKSAMKEIT.

REAGENT FOR THE DETERMINATION OF ALKALINE PHOSPHATASE IN SERUM

1,273,506. Determination of alkaline phosphatase. MERCK PATENT G.m.b.H. 12 Feb., 1970 [4 March, 1969], No. 6901/70. Heading B1X. A reagent for determination of alkaline phosphatase in serum comprises an aqueous solution of para-nitrophenyl phosphate containing diethanolamine hydrochloride in a molar concentration of at least 1.5 and at most 4.5. In an Example the reagent also contains sodium azide and magnesium chloride and is adjusted to a pH of 9.8. In carrying out a test the serum sample and the reagent are mixed and the rate of change of extinction of the mixture is measured at 405 nm.

TEST COMPOSITION FOR DETERMINING ELECTROLYTES

PROCEDURE AND REAGENT FOR THE PROOF OF IONS USING MALTOSE DERIVATIVES

PROCEDURE AND COMPOSITION FOR THE PROOF OF SODIUM IONS IN LIQUIDS

PROCEDURE FOR THE PRODUCTION OF A MODIFIED ONE OLIGOSACCHARIDS

NEW INHIBITORS FOR HYDROLYTIC ENZYMES AND SUBSTRATES AND REGULATION PROCEDURE, SELBIGE INCLUDING PROCEDURES AND TEST RECORDS

STURDY ALPHA AMYLASE REAGENT.

HETEROBIFUNCTIONAL ANTIBODIES WITH BOTH CATALYTIC CHARACTERISTICS AND SPECIFIC ANTIGEN CONNECTION CHARACTERISTICS AND PROCEDURES FOR THEIR USE

PROCEDURE FOR THE PRODUCTION OF NEW N-ACYLIERTEN AMINO ACID ESTERS

PROCEDURE FOR THE REGULATION OF ALPHA AMYLASE UNDER USE OF MODIFIED OLIGOSACCHARIDEN AND PROCEEDING FOR THEIR PRODUCTION.

SUBSTRATES FOR BETA-GALACTOSIDASE

SUBSTRATES FOR BETA-GALACTOSIDASE

COMPOSITION FOR THE ANALYSIS OF THE ALKALINE PHOSPHATASE AND METHOD THEREFOR

COMPOSITION FOR THE ANALYSIS OF THE ALKALINE PHOSPHATASE AND METHOD THEREFOR . A novel composition is disclosed for determining the activity of the alkaline phosphatase, which comprises a salt of p-nitrophenylphosphoric acid, a magnesium salt, sodium borate and 2-amino--2-hydroxymethyl-1,3-propanediol. The composition affords advantages over the known composition especially as regards sensitivity.

OLIGOSACCHARIDE COMPOUNDS AND PREPARATION THEREOF

OLIGOSACCHARIDE COMPOUNDS AND PREPARATION THEREOF Novel silyl blocked oligosaccharide compounds useful in assays for detecting aamylase are disclosed along with methods for their production and use. The most preferred compound comprises 6-O-thexyldimethylsilyl-4-nitrophenyl-.alpha.-D-maltoheptaosi de.

HYDROLYTIC ENZYME INHIBITORS AND SUBSTRATES AND ASSAYS, METHODS AND KITS EMBODYING SAME

The present invention provides novel hydrolytic enzyme inhibitors (inactivators) and substrates that have three functional parts: 1) a moiety that is specifically cleavable upon contact of the compound with a specific hydrolytic enzyme which moiety when cleaved is detectable and measurable, 2) a moiety that is recongnized by a specific hydrolytic enzyme and which interacts therewith and the substrate is specifically cleavable upon such interaction and 3) a linker moiety linking moieties 1) and 2). These novel compounds are useful in assays to detect and mesure levels of hydrolytic enzyme activity and are also useful in treatment regimens for various disease states and conditions implicating the underlying specific hydrolytic enzyme. Examples of hydrolytic enzymes include, but are not limited to, phopholipases, lipases, esterases, proteases, etc.

COMPOSITION POUR LA DETERMINATION DE LA PHOSPHATASE ACIDE PROSTATIQUE ET PROCEDE D'UTILISATION DE CETTE DERNIERE

LA PRESENTE INVENTION CONCERNE UNE COMPOSITION QUI PERMET DE DETERMINER LA PHOSPHATASE ACIDE PROSTATIQUE D'UNE MANIERE SPECIFIQUE, RAPIDE ET SIMPLIFIEE ET QUI EST COMPOSEE D'UN SERUM SPECIFIQUE ANTI-PHOSPHATASE ACIDE PROSTATIQUE, D'UN SERUM SPECIFIQUE ANTI-GAMMA-GLOBULINE DE L'ESPECE ANIMALE UTILISEE POUR LA PREPARATION DU SERUM ANTI-PHOSPHATASE-ACIDE PROSTATIQUE (CES SERUMS POUVANT ETRE DILUES DANS UNE SOLUTION-TAMPON APPROPRIEE), D'UN SUBSTRAT APPROPRIE ET D'UN REACTIF DE BLOCAGE DE L'ACTIVITE ENZYMATIQUE. L'INVENTION CONCERNE EGALEMENT LE PROCEDE DE DETERMINATION DE L'ACTIVITE DE LA PHOSPHATASE ACIDE PROSTATIQUE A L'AIDE DE LA COMPOSITION CI-DESSUS.

ASSAY REAGENTS

EXTRAITS BIOLOGIQUES NOTAMMENT DE LIMULUS, DEPOURVUS D'INHIBITEURS ET COAGULABLES PAR UNE ENDOTOXINE ET PROCEDE DE LEUR PREPARATION

L'INVENTION CONCERNE L'INDUSTRIE BIOCHIMIQUE. POUR STANDARDISER DES EXTRAITS COAGULABLES PAR UNE ENDOTOXINE ON INACTIVE L'INHIBITEUR ENDOGENE, ON DETERMINE L'ACTIVITE COAGULASIQUE DE L'EXTRAIT ET ON INTRODUIT DES QUANTITES PROPORTIONNEES DE L'EXTRAIT DANS UN MELANGE REACTIONNEL DE DETERMINATION D'UNE ENDOTOXINE DE FACON A OBTENIR UNE VALEUR PREDETERMINEE DE L'ACTIVITE COAGULASIQUE; DANS LA PLUPART DES CAS, ON DETERMINE EGALEMENT LA CONCENTRATION EN COAGULOGENE ET SI ELLE EST INFERIEURE A UNE VALEUR PREDETERMINEE, ON AJOUTE DU COAGULOGENE A L'EXTRAIT; DE FACON AVANTAGEUSE, POUR INACTIVER L'INHIBITEUR, ON AJUSTE LE PH DE L'EXTRAIT A UNE VALEUR FORTEMENT ALCALINE, ON INCUBE ET ON REAJUSTE LE PH AU VOISINAGE DE LA NEUTRALITE. APPLICATION NOTAMMENT AUX EXTRAITS D'AMIBOCYTES DE LIMULUS.

N-Carboxyacylaminoacid esters, processes for their production and their diagnostic use

The invention relates to N-carboxyacylaminoacid esters, a process for their production and their use as chromogenic substrates for the continuous and discontinuous determination of enzymes with a chymotrypsin-like specificity, in particular catheepsin G.

IDENTIFICATION OF LIGANDS BY SELECTIVE AMPLIFICATION OF CELLS TRANSFECTED WITH RECEPTORS

Способ получения @ -нитрофенил- @ -мальтогептаозида

A substrate for the determination of alpha -amylase of the formula I is prepared by reacting the particular phenyl glucoside or nitrated phenyl glucoside with alpha -cyclodextrin, amylase or soluble starch in the presence of Bacillus macerans amylase. The radical R in the formula I has the meaning stated in the claim. <IMAGE>

Verfahren und Zusammensetzung zum Nachweis von Natrium-Ionen in Flüssigkeiten

Verfahren und Zusammensetzung zum Nachweis von Chlorid-Ionen in Flüssigkeiten

VERFAHREN UND REAGENZ ZUR BESTIMMUNG VON ALPHA-AMYLASE.

Determination of alpha-amylade (AA) comprises cleavage of a substrate in presence of at least one auxiliary enzyme, then measuring the cleavage products. The new feature is that reaction is carried out in presence of a monoclonal antibody (MAB) against AA. Also new are reagents for this process. Pref. the substrate is a D-maltooligoside of 2-10, esp. 4-8, glucose units, opt. substd. by a chromophore (esp. nitrophenyl) and/or having at least one ethylidene protecting gp. MAb are esp. derived from the hybridoma clones ECACC 86020601 and ECACC 86020602.

Inactivating inhibitors in endotoxin clottable extracts

Endotoxin clottable extracts are standardized by inactivating endogenous inhibitor by adjusting the extract pH to above 8.5, incubating and readjusting the pH to near neutrality, determining the coagulase activity of the extract and dividing the extract into endotoxin assay reaction mixture portions each providing coagulase activity at a predetermined level. In most circumstances the coagulogen concentration is also determined and, if the concentration is lower than a predetermined amount, coagulogen is added to the extract.

ENHANCED CHEMILUMINESCENT ASSAY

PROCEDURE AND REAGENT FOR THE REGULATION OF ALPHAAMYLASE.

OLIGLUCOSIDDERIVATE.

NEW BETA GALACTOSIDASE SUBSTRATES FOR THE CEDIA

PROCEDURE FOR THE REGULATION OF ALKALINE PHOSPHATASE

VERFAHREN ZUR HERSTELLUNG VON NEUEN N-ACYLIERTEN AMINOSAEUREESTERN

OLIGOGLUCOSIDE DERIVATIVES

HEPTAOSE COMPOUNDS

LABORATORY REAGENT FOR ASSAY OF ALKALINE PHOSPHATASE

HETEROBIFUNCTIONAL ANTIBODIES POSSESSING DUAL CATALYTIC AND SPECIFIC ANTIGEN BINDING PROPERTIES AND METHODS USING THEM

A novel and useful class of antibody molecules is described which possess the characteristics of an antigen- specific antibody and a catalytic antibody. Such a hybrid molecule may be assembled in vitro using biochemical means or using in vivo methods including cell hybridization, or recombinant DNA technologies. The product of the invention possesses both antigen target specificity as well as an intrinsic catalytic activity. Further said product may be used in any appropriate application including diagnostic test as a substitute for an enzyme-labelled antibody conjugate or as a therapeutic reagent whereby a part of the antibody molecule recognizes a unique cellular marker and the catalytic portion of antibody molecule has therepeutic activity.

HETEROBIFUNCTIONAL ANTIBODIES POSSESSING DUAL CATALYTIC AND SPECIFIC ANTIGEN BINDING PROPERTIES AND METHODS USING THEM

A novel and useful class of antibody molecules is described which possess the characteristics of an antigenspecific antibody and a catalytic antibody. Such a hybrid molecule may be assembled in vitro using biochemical means or using in vivo methods including cell hybridization, or recombinant DNA technologies. The product of the invention possesses both antigen target specificity as well as an intrinsic catalytic activity. Further said product may be used in any appropriate application including diagnostic test as a substitute for an enzyme-labelled antibody conjugate or as a therapeutic reagent whereby a part of the antibody molecule recognizes a unique cellular marker and the catalytic portion of antibody molecule has therepeutic activity. 34 ...

PROCESS OF DETERMINATION OF AMYLASE

COMPOSITION FOR the ANALYSIS OF PHOSPHATASE ALKALINE AND PROCEEDED FOR SA IMPLEMENTED

EXTRACTS BIOLOGICAL IN PARTICULAR OF LIMULUS, DEPRIVED Of INHIBITORS AND COAGULABLE BY a ENDOTOXINE AND PROCESS OF THEIR PREPARATION

IDENTIFICATION OF LIGANDS BY SELECTIVE AMPLIFICATION OF CELLS TRANSFECTED WITH RECEPTORS

리간드로서 작용할 수 있는 물질을 검출하는 방법으로서, (a) 세포증식을 허용하는 조건하에서, 리간드에 응답하여 세포증식에 영향을 줄 수 있는 리셉터들을 암호화하는 DNA로 트랜스펙션되고, 세포증식의 표지인자를 함유하는 세포들 리셉터의 잠재 아고니스트(agonist) 또는 길항제(antagonist)인 시험물질과 함께 배양하는 단계와 (b) 세포증식을 허용하기에 충분한 시간후에, 표지인자를 함유하지 않은 세포와 비교하여 표지인자를 함유하는 세포의 증식의 존재 또는 부재를 측정하는 단계로 이루어진다. A method of detecting a substance capable of acting as a ligand, comprising: (a) transfecting with a DNA encoding receptors capable of affecting cell proliferation in response to the ligand under conditions that allow cell proliferation and labeling of cell proliferation Incubation with a test substance that is a latent agonist or antagonist of the factor-containing cells receptor and (b) after sufficient time to allow cell proliferation, compared to cells that do not contain markers By measuring the presence or absence of proliferation of cells containing the marker.

DETECTION OF MICROORGANISMS USING GAS SENSORS

A method is provided for assessing the contamination status of materials with respect to possible presence of microorganisms, particularly pathogenic microorganisms, by measuring the production of a specific gas or vapour evolved by a sample of the material when it is incubated with a bacterial enzyme substrate. More particularly the present invention relates to the selective detection of Escherichia coli organisms in the material sample and relating the presence and/or number of these to the presence and/or number of pathogenic organisms. The method is particularly applicable to testing foodstuffs for the likely presence of pathogens. Most preferred substrates comprise o-nitrophenyl-beta-D-glucuronidde and methylsalicyly-beta-D-glucuronide. Test kits for carrying out said method are also provided.

Test implement and test method for colorimetrically determining whether a female is fertile or pregnant

A test implement and method for colorimetrically assaying the quantity of N-acetyl-β-glucosaminidase in a female biological medium, such as saliva, which quantity is indicia of fertility or pregnancy. The implement is an absorbent material, such as paper strip, impregnated with a phenolic derivative of N-acetyl-β-d-glucosamine that reacts in the presence of the glucosaminidase at an acid pH to form a phenol that has a distinct color at an alkaline pH, and a buffer that maintains said acid pH. The method may be carried out by wetting the implement with the medium, alowing the phenol to form, raising the pH to alkalinity by wetting the implement with an appropriate buffer solution, and comparing the color of the implement with a color standard.

Novel substrates for use in measuring the concentration of kallikrein in urine

A novel chromogenic and fluorescent substrate for measuring the concentration of kallikrein in urine. The novel substrate according to the present invention has very excellent substrate specificity to kallikreins and good solubility in water or biological test solutions. The present substrate is therefore useful for measuring the concentration of kallikrein in urine for the diagnosis of hypertension and other diseases caused by the lowering of the concentration of kallikrein.

Composition for the analysis of the alkaline phosphatase and method therefor

A novel composition is disclosed for determining the activity of the alkaline phosphatase, which comprises a salt of p-nitrophenylphosphoric acid, a magnesium salt, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol. The composition affords advantages over the known composition especially as regards sensitivity.

Method and reagent for the determination of alpha-amylase

A process and a reagent for the determination of alpha -amylase involve enzymatic splitting of an alpha -amylase substrate in the presence of alpha -glucosidase and beta -glucosidase and measurement of a fission product. As substrate, there is used a 4-nitrophenyl- beta ,D-maltoheptaoside electronegatively substituted in the phenyl nucleus.

Method and composition for the determination of calcium ions in fluids

A METHOD FOR INCREASING THE SENSITIVITY OF ASSAYS

Diagnostic reagents and a method for increasing the sensitivity of chemical and enzymatic analysis. In particular, an improved reagent and method wherein the sensitivity of the spectral analysis is improved by the addition of a water-soluble inclusion compound (cyclodextrin).

Beta-lactam compounds and methods of use thereof

Beta-lactam compounds to detect carbapenemases or microbial carbapenem resistance are disclosed. The compounds contain a chemical probe. Upon hydrolysis by carbapenemases, the compounds undergo intramolecular rearrangement and release the chemical probe. Detection of the released chemical probe indicates the presence of carbapenemases and the presence of microbial carbapenem resistance.

AMYLASE DETERMINATION

Dertermination of ions in fluids

내용 없음. No content.

Novel substrate for measuring kallikrein in urine

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Stable one-part α-amylase assay reagent

Method and composition for determination of sodium ions in fluids

本发明涉及生物或非生物液体中的离子(下面称 为分析物)测定的方法和组合物。本发明的理论根据 是许多分析物有激活或抑制敏感酶的作用。分析物 可以是阳离子、阴离子、金属或非金属、单质或化合 物。在实践中，经常遇到分析物在样本中的浓度超过 相关分析指示酶的敏感性范围，或存在其他离子对酶 的敏感性产生干扰。本发明从多种途径提出并解决 这些问题的方案。

Reagent composition for measuring electrolyte

(57)【要約】 【課題】液状試薬として流通に耐えうる、高い溶液安定 性を有し、さらに、定量性、正確性に優れた電解質の酵 素的測定用試薬組成物を提供する。 【解決手段】（ａ）不活性化型α−アミラーゼ、（ｂ） キレート剤、（ｃ）α−アミラーゼ基質および（ｄ）シ クロデキストリン誘導体、または該組成物に、さらに、 ＳＨ基含有化合物またはその塩を含有するカルシウムイ オンまたは塩素イオンなどの電解質測定用試薬組成物で ある。

Method and composition for the determination of sodium ions in fluids

Method of potassium ion concentration assay in biological material

FIELD: medicine. SUBSTANCE: 10 mcl plasma or serum blood taken off in patient is placed to the incubation medium of the following composition: pyruvate kinase from Bacillus stearothermophillus (50-10000 U/l), phosphoenolpyruvate as neutral tris-salt (0.3-10 mmole/l), 4,7,13,16,21-pentaoxa-1,10-diazabicyclo-8,8,5-tricosane (up to 30 mmole/l), 50-500 mM buffer, pH 8 (0.10-0.8 mmole/l), manganese or magnesium ions (1-10 mmole/l), lithium chloride (2-100 mmole/l), ADF as free acid (0.5-10 mmole/l), serum lactate dehydrogenase (5000-100000 U/l), glutamic acid dehydrogenase (2500-20000 U/l, determined at 25 C), keto-glutaric acid as free acid (1-10 mmole/l) or glycerol dehydrogenase from Enterobacter aerogenes (50-1000 U/l), glycerol (0.3-3 mmole/l), 4,7,13,16,21-pentaoxa-1,10-diazabicyclo-8,8,5-tricosane (up to 30 mmole/l), NAD (0.1-5.0 mmole/l), buffer, pH 9 (20-500 mmole/l), serum albumin (up to 5 g/l), glutamic acid dehydrogenase (2500-20000 U/l, determined at 25 C), keto-glutaric acid as free acid (1-10 mmole/l), or acetaldehyde dehydrogenase from yeast (50-10000U/l), glycol aldehyde (0.3-30 mmole/l), 4,7,13,16,21-pentaoxa-1,10-diazabicyclo-88,5-tricosane (up to 30 mmole/l). NAD (0.05-2.0 mmole/l), buffer, pH 7-8 (50-500 mmole/l), dithiothreitol (0.1-2 mmole/l), serum albumin (up to 5 g/l), glutamic acid dehydrogenase (2500-20000 U/l, determined at 25 C), keto-glutaric acid as free acid (1-10 mmole/l), or acetaldehyde dehydrogenase (50-10000 U/l), 4-nitrophenyl acetate (0.3-30 mmole/l), 4,7,13,16,21-pentaoxa-1,10-diazabicyclo-8,8,5-tricosane (up to 30 mmole/l), NAD (0.001-0.1 mmole/l), buffer, pH 7-8 (50-500 mmole/l), dithiothreitol (0.1-2 mmole/l), serum albumin (up to 5 g/l), glutamic acid dehydrogenase (2500-20000 U/l, determined at 25 C), keto-glutaric acid as free acid (1-10 mmole/l). Then incubation mixture is incubated at 37 C for 0.1-5 min followed spectrophotometry at 340 nm and potassium ions measurement by calibration curve. EFFECT: improved method of ...

Determination of ions in fluids

本发明涉及一种测定体液中钾离子的方法，其特征在于测定这些离子对微生物转移酶、微生物氧化还原酶、裂解酶或水解酶的活性的影响。本发明也涉及用于测定体液中钾离子的组合物，该组合物包括活性受钾离子影响的微生物转移酶或微生物氧化还原酶。

Monolithic multilayered analytical element for measuring activity of alkaline phosphatase

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Analytical method and reagent of alpha-amylase

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Integrated type multilayer analysis element for measuring alkaline phosphatase activity

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

SUBSTRATES FOR USE IN DETERMINING THE CONCENTRATION OF URINALLIC REIN.

.alpha. AMYLASE ASSAY

Ab?tract of the Disclosure Method of measuring the amount of .alpha. -amylase in a liquid sample, including the steps of providing an oligosaccharide substrate for .alpha.-amylase, the substrate being characterized in that it contains at least 3 glucose units, its reducing-end glucose unit is bonded, via a bond cleavable by .alpha.-or .beta. -glucosidase, to a label which exhibits an optically measurable change upon cleavage of the bond, and its terminal glucose unit is bonded to a blocking substituent which inhibits cleavage by exo-enzymes of the bond between the terminal glucose unit and the adjacent glucose unit; contacting the sample with the oligosaccharide substrate and with a first exo-enzyme capable of cleaving the bond between the reducing-end glucose unit and the label, and measuring the optically measurable change as a measure of .alpha. -amylase in the sample.

COMPOSITION FOR THE ANALYSIS OF ALKALINE PHOSPHATASE AND METHOD FOR ITS IMPLEMENTATION

Composition pour la détermination de l'activité de la phosphatase alcaline. La composition comprend un sel de l'acide p-nitrophényl-phosphorique, un sel de magnésium, la borate de sodium et le 2-amino-2-hydroxyméthyl-1,3-propanediol. Composition for the determination of the activity of alkaline phosphatase. The composition comprises a salt of p-nitrophenyl-phosphoric acid, a salt of magnesium, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol.

Patent FR2201299A1

AMYLASE DETERMINATION PROCESS

L'invention concerne un procédé pour la détermination de l'amylase. Pour déterminer l'amylase on clive des substances chromogènes dont le chromophore est une substance colorimétrique fluorescente ou absorbant les ultraviolets, telles que des dérivés du p-nitrophénol et d'oligosaccharides dont la chaîne comporte 4 à 10 motifs de glucose; les oligosaccharides dont la chaîne a une telle longueur résistent au clivage par l' alpha - et/ou la beta -glucosidase et comportent des liaisons endo- alpha -1,4 nécessaires à la manifestation de l'activité amylasique; le clivage des liaisons alpha -1,4 par l'amylase forme des fragments plus petits sur lesquels l' alpha -glucosidase et/ou la beta -glucosidase agissent pour libérer le chromophore; la vitesse de libération du chromophore est proponionnelle à l'activité amylasique et permet des déterminations selon une méthode cinétique ou une méthode du point final. L'invention s'applique en particulier à des déterminations qualitatives ou quantitatives de l'amylase dans des échantillons utiles pour le diagnostic médical. The invention relates to a method for the determination of amylase. To determine amylase, chromogenic substances whose chromophore is a fluorescent colorimetric substance or which absorbs ultraviolet rays, such as derivatives of p-nitrophenol and oligosaccharides, the chain of which contains 4 to 10 glucose units, are cleaved; oligosaccharides whose chain has such a length resist cleavage by alpha - and / or beta -glucosidase and contain endo-alpha -1,4 bonds necessary for the manifestation of amylase activity; cleavage of alpha -1,4 bonds by amylase forms smaller fragments on which alpha -glucosidase and / or beta -glucosidase act to release the chromophore; the rate of chromophore release is proportional to amylase activity and allows determinations by a kinetic method or an end point method. The invention is particularly applicable to qualitative or quantitative ...

Sugar ester derivatives, reagents for measurement of hydrolase activity and methods for measurement of hydrolase activity

A sugar ester derivative of the general formula (I) wherein G stands for a group of the formula (A) or a group of the formula (B) wherein t means 0 or 1; at least one of R 1 and R 2 means an ester residue of a saturated or unsaturated fatty acid having 1 - 30 carbon atoms and the other group means hydrogen atom or acetyl group, and R 3 means a group of the formula (C) wherein X means a halogen atom, m means an integer of 0 to 4, Y means hydroxy group, an alkoxy group, a carboxyl group or sulfonic acid group, n means 0 or 1 and Z means nitro group or nitrovinyl group and its use. The sugar ester derivative of the general formula (1) is useful as a substrate for the measurement of lipase or esterase activities, and the reagents and the methods for measuring lipase or esterase activities which comprise said sugar ester derivative as the substrate are excellent in terms of reproducibility and sensitivity and enable the measurement of lipase or esterase activities in accordance with rate-assay method by an easy procedure.

N-carboxyacylamino-acid esters, process for their preparation and their use

Quantitative determination of amylase

Oligosaccharide derivatives suitable for .alpha.-amylase determination

ABSTRACT OF THE DISCLOSURE An oligosaccharide derivative of tne formulas [I] wherein R is -SR2, -?-R2 or -?-R2; R1 is an optically detectable group, etc.; R2 is alkyl or substituted alkyl; and n is zero or 1-5, is effective as a substrate for measuring .alpha.-amylase activity and can be synthesized easily in high yield.

Methods for detecting LP-PLA2 activity and inhibition of LP-PLA2 activity

This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.

PROCEDURE FOR DETERMINING FLUIDS IONES.

METHOD AND TEST EQUIPMENT TO DETERMINE AMYLASE CONTENT OF A SAMPLE

Biological extracts and method for making same

A p-nitrophenyl hydrolase contaminant present in Limulus coagulase sources interferes in, i.e., inhibits, coagulase-based assays for endotoxin. The contaminant inhibitor enzyme may be removed from coagulase by physical separation or by selective denaturation, for example by adjusting the pH of a coagulase solution to about from 8.5 to 11. Inhibitor-free coagulase is a novel composition. The coagulase activity of the composition is adjusted to a predetermined level to provide a standardized coagulase reagent for use in endotoxin assays.

Methods for detecting Lp-PLA2 activity and inhibition of Lp-PLA2 activity

This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.

Ph-indicator based assay for selective enzymes

Provided herein is a method for the quantitative screening of hydrolase for desired substrate activity using pH indicators which are sensitive to the release of protons from a chemical reaction in a reaction mixture. The metho d comprises selecting buffer and indicator conditions such that both have the same affinity for protons such that the relative amount of buffer protonated is proportional to the amount of indicator protonated as the pH of the reaction mixture shifts. A reaction mixture is then prepared comprising a buffer, indicator, hydrolase to be tested, and desired substrate to be teste d, allowing the hydrolase to react with the substrate. The reaction is monitore d by detection of change in color of the reaction mixture, determined by the affect of the reaction on the indicator.

Method for increasing the sensitivity of assays

Abstract of the Invention This invention relates to diagnostic reagents and a method for increasing the sensitivity of chemical and enzymatic analysis. In particular, it relates to an improved reagent and method wherein the sensitivity of the analysis is improved by the addition of a water-soluble inclusion compound.

Method and reagent for the determination of alpha-amylase

NEW ANALYTICAL CONNECTIONS AND THEIR USE

Test reagent for quantification of amylase

Process and reagent for the determination of alpha-amylase

Heptaose compounds

ABSTRACT Heptaose compounds of the general formula: (I) in which R and R1, independently of one another, each represent a straight-chained or branched alkyl or alkanoyl radical containing up to 6 carbon atoms or a phenyl radical or R and R1 together also form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, R2 represents an oligoglucoside residue containing 2 to 7 glucose units and X is a hydrogen atom or an optically-determinable residue; compounds (I) are useful as substrates for the deter-mination of .alpha.-amylase which is important in clinical testing of the pancreas function.

Method of identifying alpha-amylase

Composite substance for measuring activity of alkaline phosphatase

PROCEDURE AND MATERIAL FOR DETERMINATION OF SODIUM IONES IN FLUIDS

Alpha amylase assay

Reagent for the determination of chloride ions

The subject matter of the invention is a reagent for the determination of chlorine ions which com­prises a compound capable of forming a chelate with calcium ions, deactivated α-amylase, a calcium chelate compound and an α-amylase activity-measuring substance.

Sugar ester derivatives, reagents for measurement of hydrolase activity and methods for measurement of hydrolase activity

A sugar ester derivative of the general formula (I) ##STR1## wherein G stands for a group of the formula (A) ##STR2## or a group of the formula (B) ##STR3## wherein λ means 0 or 1; at least one of R 1 and R 2 means an ester residue of a saturated or unsaturated fatty acid having 1-30 carbon atoms and the other group means hydrogen atom or acetyl group, and R 3 means a group of the formula (C) ##STR4## wherein X means a halogen atom, m means an integer of 0 to 4, Y means hydroxy group, an alkoxy group, a carboxyl group or sulfonic acid group, n means 0 or 1 and Z means nitro group or nitrovinyl group and its use. The sugar ester derivative of the general formula (I) is useful as a substrate for the measurement of lipase or esterase activities, and the reagents and the methods for measuring lipase or esterase activities which comprise said sugar ester derivative as the substrate are excellent in terms of reproducibility and sensitivity and enable the measurement of lipase or esterase activities in accordance with rate-assay method by an easy procedure.

N-carboxyacylaminoacid esters, processes for their production and their diagnostic use

Bayer 3644-JGK ABSTRACT OF THE DISCLOSURE The invention relates to N-carboxyacylaminoacid esters, a process for their production and their use as chromogenic substrates for the continuous and discontinuous determination of enzymes with a chymotrypsin-like specifi-city, in particular catheepsin G.

Method for classifying the presence or absence of a microorganism in a biological medium

本发明涉及一种用于确定存在或不存在微生物的方法，所述方法包括以下步骤：1）提供含有液相或半固相和气相的容器，所述液相或半固相含有生物培养基，所述生物培养基能够含有活形式的所述微生物、营养元素、和对于所述微生物是特异的且可被代谢成至少一种VOC代谢物的酶底物，所述气相与所述液相或半固相邻近；2）将至少所述液相或半固相暴露于适合于所述微生物将所述酶底物代谢成所述VOC代谢物的分子的条件中；和3）通过光学转换，确定存在或不存在所述VOC代谢物，特征在于，所述VOC代谢物与纳米多孔基体相互作用，所述基体以与所述酶底物分开的形式而实现，且特征在于，通过光学转换对所述基体的光学性质的变化的检测，表明所述基体与所述代谢物相互作用。

COMPOSITION SUITABLE FOR THE ANALYSIS OF ALKALINE PHOSPHATASE AND METHOD USING THE SAME

Determination of ions in fluids

The invention concerns a process and a composition for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured and a binding agent is added for masking of interfering ions. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium substances that give rise to ammonium. The enyzmes which are used may be for example a transferase, a hydrolase, an oxidoreductase or a lyase.

Detection of ions in liquids

Heterobifunctional antibodies possessing dual catalytic and specific antigen binding properties and methods using them

Method and composition for the determination of sodium ions in fluids

The invention concerns a process and a composition for the determination of sodium ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The enzymes which are used may be for example a transferase, a hydrolase or an oxidoreductase. An essential part of the invention is a method to exclude interferences by ions by masking the interfering ions with a binding agent.

Method and test kit for serum amylase assay

ABSTRACT OF THE DISCLOSURE Disclosed herein are a method and a reagent test kit, both using an improved substrate to measure the amylase content of a sample. The substrate used is a glycoside and a substituted aromatic radical attached to the terminal unit of the glycoside. When detached from the polysaccharide, the aglycone exhibits a different spectral absorbance than the substrate.

Process and reagent for the determination of α-amylase

α-Amylase is determined by the enzymatic splitting of an α-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by α-amylase, are also provided.

Staphylococcus aureus fluorescence developing culture medium

本发明公布了一种用于检测金黄色葡萄球菌的荧光显色培养基，含有氮源、碳源、氯化钠、杂菌生长抑制剂，其特征在于，还包括金黄色葡萄球菌显色底物4‑甲基伞型酮‑对硝基β‑D‑葡萄糖苷、β‑D‑葡萄糖苷，非金黄色葡萄球菌显色底物对硝基酚‑α‑D‑吡喃半乳糖，非金黄色葡萄球菌显色底物对硝基苯酚β‑D‑葡萄糖醛酸苷。本发明公布的荧光显色培养基在助显色剂不存在的情况下，为区分金黄色葡萄球菌和其他葡萄球菌的提供更简便的显色方案，能有效区分黄色葡萄球菌与部分非金黄色葡萄球菌及其他杂菌，对黄色葡萄球菌具有较好的选择性。

Method and composition for the determination of bicarbonate ions in fluids

The invention concerns a process and a composition for the determination of bicarbonate ions in fluids, wherein the influence on these ions of the activity of an enzyme is measured. The enzymes which are used may be, for example, a transferase or a hydrolase.

Stable single liquid alpha-amylase reagent

A stable, single liquid alpha-amylase assay reagent comprises a polysaccharide or long chain oligosaccharide substrate for alpha-amylase having an optically detectable label bonded to the reducing end glucose, by a bond cleavable by alpha- or beta-glucosidase, and a blocking group bonded to the terminal end glucose. The reagent further comprises beta-amylase and either alpha- or beta-glucosidase. Sorbitol is provided in an amount of about 5% to retard degradation of the enzymes. Zwitterionic buffers are also provided to stabilize the enzymes. In preparing the reagent, the enzymes and substrate are filtered through a 0.2 micron or less filter to remove alpha-amylase producing organisms.

Analytical element and method for theophylline determination using buffer in spreading zone

BIOLOGICAL EXTRACTS, IN PARTICULAR FROM LIMULUS, FREE OF INHIBITORS AND COAGULABLE BY AN ENDOTOXIN AND PROCESS FOR THEIR PREPARATION

Identification of ligands by selective amplification of cells transfected with receptors

The invention is directed to a method for identifying substances acting as ligands for transfected receptors by using transfected markers to measure receptor/ligand interactions.

PROCEDURE AND REAGENTS FOR DETERMINATION OF ALFA-AMYLAS

Patent JPH0522518B2

Method of determining potassium ions in liquids and agent suitable forsaid method

Determination of ions in fluids

Method for measuring the concentration of an enzyme by photometric absorption measurement of an enzymatically formed dye in a measuring solution

Described is a method of determining the concentration of an enzyme by reacting the enzyme with a substrate solution and measuring the concentration of the dye thus formed by photometric measurement of the absorption of a test solution, the measurement being carried out in the presence of a second dye which is added to the test solution and which does not interfere with the measurement of the concentration of the dye formed by the enzyme. For the determination of alkaline phosphatase from a biological sample or from an enzyme immuno-assay, the preferred dye is amaranth, and it is preferably added to the test solution together with a timer solution to time the enzymatic dye-forming reaction.

Oligosaccharide derivatives suitable for alpha-amylase determination

An oligosaccharide derivative of the formula : wherein R is -SR 2 , R 1 is an optically detectable group, etc. ; R 2 is alkyl or substituted alkyl ; and n is zero or 1-5, is effective as a substrate for measuring α-amylase activity and can be synthesized easily in high yield.

Assay reagents

1392403 Nitrophenyloxy substituted alkane derivatives SYNTEX (USA) Inc 30 Jan 1973 [10 Feb 1972] 4654/73 Headings C2C and C2P [Also in Division G1] The invention comprises compounds of formula wherein n is 0 or 1; R<SP>1</SP> represents p-nitrophenoxy, o,p - dinitrophenoxy, fluorescein, methoxyfluorosceine, o - aminobenzoate, coumarins, phenols or aromatic compounds iodinated with I<SP>125 </SP>or labelled with C<SP>11</SP> carbon; R<SP>2</SP> is H, or -CONH-(Antigen or Antibody) or -COCH 2 -NH-(Antigen or Antibody), the term "antibody" or antigen means hormones, peptides and drugs; R<SP>10</SP> represents -OH, alkaline and acid phosphomonoesterases, sulphatases, esterases, peptidases, proteinases, carbohydrases, phosphate esters and ureas; R<SP>11</SP> represent -OH, -NH 2 , a mercapto or phosphate ester group or R<SP>10</SP>. Many compounds are prepared in the examples. Example 13 describes the preparation of those compounds of above formula in which R<SP>10</SP> and/or R<SP>11</SP> represent a phosphate ester or a phosphatamino group.

Process for preparing a maltoheptaose derivative

α-Amylase is determined by the enzymatic splitting of an α-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by α-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.

Amylase determination

AMYLASE DETERMINATION Abstract A method for the determination of amylase, based on the cleavage of colorimetric, ultraviolet absorbing or fluorometric substances, commonly referred to as chromogenic substances as p-nitrophenol derivatives of oligo-saccharides of chain length 4-10 glucose units. Oligosaccharides of this chain length are resistant to cleavage by .alpha. and/or .beta.-glycosidase and contain endo-.alpha.-1,4 linkages which are required for amylase activity. Cleavage of the .alpha.-1,4 bonds by amylase produces smaller fragments which are acted uponby .alpha.-glucosidage and/or .beta.-glucosidase to liberate a chromophore. The rate of appearance of the chromophore is proportional to amylase activity and lends itself to either rate or endpoint determinations. The qualitative or quantitative measurement of amylase concentrations in a sample is useful in medical diagnosis.

Determination of ions in fluids

A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.

Method for determining alkaline phosphatase

Method for the determination of alpha-amylase using modified oligosaccharides and method for their production.

COMPOSITION FOR DETERMINING ACID PROSTATE PHOSPHATAS AND USE THEREOF

COMPOSITION FOR DETERMINING PROSTATIC ACID PHOSPHATASE AND METHOD OF USING THE SAME

LA PRESENTE INVENTION CONCERNE UNE COMPOSITION QUI PERMET DE DETERMINER LA PHOSPHATASE ACIDE PROSTATIQUE D'UNE MANIERE SPECIFIQUE, RAPIDE ET SIMPLIFIEE ET QUI EST COMPOSEE D'UN SERUM SPECIFIQUE ANTI-PHOSPHATASE ACIDE PROSTATIQUE, D'UN SERUM SPECIFIQUE ANTI-GAMMA-GLOBULINE DE L'ESPECE ANIMALE UTILISEE POUR LA PREPARATION DU SERUM ANTI-PHOSPHATASE-ACIDE PROSTATIQUE (CES SERUMS POUVANT ETRE DILUES DANS UNE SOLUTION-TAMPON APPROPRIEE), D'UN SUBSTRAT APPROPRIE ET D'UN REACTIF DE BLOCAGE DE L'ACTIVITE ENZYMATIQUE. L'INVENTION CONCERNE EGALEMENT LE PROCEDE DE DETERMINATION DE L'ACTIVITE DE LA PHOSPHATASE ACIDE PROSTATIQUE A L'AIDE DE LA COMPOSITION CI-DESSUS.

Diagonostic composition containing stabilizaed liquid phosphate and preparation thereof

Patent FR2402872B1

Sheltering of interfering ion

本发明涉及生物或非生物液体中的离子(下面称 为分析物)测定的方法和组合物。本发明的理论根据 是许多分析物有激活或抑制敏感酶的作用。分析物 可以是阳离子、阴离子、金属或非金属、单质或化合 物。在实践中，经常遇到分析物在样本中的浓度超过 相关分析指示酶的敏感性范围，或存在其他离子对酶 的敏感性产生干扰。本发明从多种途径提出并解决 这些问题的方案。

DETERMINATION OF IONS IN FLUIDA

Determination of potassium ions in fluids

A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.

Oligosaccharide derivatives for alpha amylase determination

Determination of potassium ions in fluids

The invention concerns a process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium substances that give rise to ammonium. The enzymes which are used may be for example a transferase, a hydrolase, an oxidoreductase or a lyase. An essential part of the invention is a method to exclude interferences by ions by masking the interfering ions with a bindig agent.

Patent JPH0229320B2

Laboratory reagent for assay of alkaline phosphatase

THE SENSITIVITY OF THE KNOWN ASSAY FOR ALKALINE AND ACID PHOSPHATASES, EMPLOYING STABLE SALTS OF P-NITROPHENYL PHOSPHORIC ACID AS THE SUBSTRATE, IS SIGNIFICANTLY ENHANCED BY THE INCORPORATION INTO REAGENT ASSAY OF A POLYHYDRIC ALCOHOL, TYPICALLY MANNITOL.

Method and reagent for determinng exo-type saccharide hydrolase activity

According to this invention are provided a method for determining an exo-type saccharide hydrolase activity which comprises adding to a sample a β-maltoside derivative represented by the general formula ##STR1## wherein R is a chromophoric organic group, as a substrate, conducting reaction in the presence of a coupled enzyme, and optically determining the substance liberated, and a method for differentially determining glucoamylase and α-glucosidase activities which comprises adding, to a sample, the above β-maltoside derivative as the first substrate and a coupled enzyme, and an α-glucoside derivative represented by the general formula ##STR2## wherein R' is a chromophoric organic group, as the second substrate, subjecting the mixtures to separate enzymatic reactions, and then optically quantitatively determine the substances liberated.

Method and reagent for the determination of alpha-amylase

alpha-Amylase is determined by cleavage of a substrate such as a D-maltooligoside with 2 to 10 glucose units, which is optionally substituted by a chromophoric group and/or carries at least one ethylidene protective group, in the presence of one or more auxiliary enzymes and measurement of a cleavage product, the reaction being carried out in the presence of monoclonal antibodies against alpha-amylase. <IMAGE>

Integral multilayer analytical element for use in the measurement of alkaline phosphatase acitivity

An integral multilayer analytical element for use in the measurement of alkaline phosphatase activity, which comprises a porous spreading layer of woven fabric or knitted fabric containing an alkaline phosphatase-sensitive self-developing substrate, a buffer layer containing one or more compounds functioning as a phosphoric acid acceptor and a buffering agent and a support layer in a laminated form.