Square wave thermal cycling

SQUARE WAVE THERMAL CYCLING

Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Square wave thermal cycling

Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Square wave thermal cycling

Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Square wave thermal cycling

Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

SQUARE WAVE THERMAL CYCLING

Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids. 114-. (canceled)15. A system for determining the melting temperature of a duplex nucleic acid molecule , said system comprising:a programmable thermal cycling apparatus;a detector; and subjecting a solution comprising a double-stranded nucleic acid molecule to a square wave temperature gradient, said gradient comprising a range of temperatures that causes at least one transition of said double-stranded nucleic acid molecule to a single-stranded nucleic acid molecules; and', 'detecting said at least one transition over the course of said square wave temperature gradient., 'a program for square wave thermal cycling, said program comprising instructions for16. The system of claim 15 , wherein said square wave temperature gradient comprises a series of temperature pulses claim 15 , each pulse comprising:a) increasing the temperature of said solution to a first high temperature;b) decreasing the temperature of said solution to a second low temperature;wherein said first high temperature is greater than said second low temperature; andwherein, in subsequent pulses, said first high temperature and said second low temperature are each adjusted to be greater than the first and second temperatures, respectively, in prior pulses.17. The system of claim 16 , wherein at least one of said temperature pulses comprises a range of temperatures wherein a detectable transition occurs between said double-stranded nucleic acid molecule and said single-stranded nucleic acid molecules.18. The system of claim 16 , wherein said detecting occurs at said first high temperature and said second low temperature in each of said temperature pulses.19. The system of claim 16 , wherein said program further comprises instructions for:identifying a temperature pulse having a maximum detectable transition from double-stranded nucleic acid to single- ...

Through the reagent container to thermal convection quantitative polymerase chain reaction device

Square wave thermal cycle

CONVECTIVE PCR DEVICE

The present invention discloses a convective PCR apparatus by using a transparent conductive thin film to replace the traditional metal heater. The PCR reaction is activated when the container with reagents contacted the heated transparent conductive thin film and the temperature inside the container raised to initiate the convective circulation. Also, the present invention could apply for a quantitative PCR reaction by adding a specific probe, fluorescent dye, light source, or photon receiver.

Convective PCR device

The present invention discloses a convective PCR apparatus by using a transparent conductive thin film to replace the traditional metal heater. The PCR reaction is activated when the container with reagents contacted the heated transparent conductive thin film and the temperature inside the container raised to initiate the convective circulation. Also, the present invention could apply for a quantitative PCR reaction by adding a specific probe, a fluorescent dye, a light source, or a photon receiver.

Square wave thermal cycling

Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

SQUARE WAVE THERMAL CYCLING

Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Analysis of Chromatin Using a Nicking Enzyme

Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample. 142.-. (canceled)43. A method for detecting abnormal cells in or from a tissue section or biopsy wherein the abnormal cells have altered chromatin compared to normal cells , comprising:(a) reacting the cells with a composition comprising a mixture of a nicking enzyme, four dNTPs, and at least one labeled dNTP, and a polymerase to selectively label the chromatin;(b) incorporating the labeled dNTP into the chromatin of the cells to form labelled nucleic acid; and(c) analyzing the pattern of labelled dNMPs in the labeled cells.44. The method according to claim 43 , wherein (c) further comprises determining whether the pattern of labelled dNMPs correspond to a cancer diagnosis of the cells or tissues.45. The method according to claim 43 , wherein prior to (a) claim 43 , making the cells permeable.46. The method according to claim 43 , wherein the tissue section or biopsy is fixed.47. The method according to claim 45 , wherein the tissue section or biopsy is fixed.48. The method according to claim 43 , wherein after (b) reverse cross linking and isolating DNA from the cells.49. The method according to claim 43 , wherein following (b) claim 43 , labelling nuclei from the cells with a primary antibody.50. The method according to claim 49 , wherein after labelling with a primary antibody claim 49 , labelling with a secondary antibody.51. The method according to claim 49 , further comprising: visualizing the labelled nuclei using a fluorescent microscope. The mammalian genome is largely packaged into chromatin ...

Resolving spatial arrays using deconvolution

Methods for determining a location of a feature on a spatial array include (a) providing an array of features on a substrate, where a feature of the array includes a barcoded oligonucleotide having, in a 5′ to 3′ direction, a spatial barcode, a cleavage domain, and a constant sequence; (b) hybridizing a priming oligonucleotide to the constant sequence; (c) extending the priming oligonucleotide using the barcoded oligonucleotide as a template; and (d) determining all or a portion of a sequence of the extended priming oligonucleotide corresponding to the spatial barcode, or a complement thereof, and a location of the extended priming oligonucleotide, and using the location of the extended priming oligonucleotide to determine the location of the feature on the spatial array.

Resolving spatial arrays using deconvolution

Methods for determining a location of a feature on an array include: (a) providing a first array with a first plurality of features immobilized on a first substrate; (b) providing a second array with a second plurality of features immobilized on a second substrate; (c) aligning the first array with the second array; (d) hybridizing a first barcoded oligonucleotide of the first array to a second barcoded oligonucleotide of the second array, thereby producing a combined nucleic acid that includes first and second spatial barcodes; (e) determining all or a portion of the sequence of the combined nucleic acid; and (f) identifying the second barcoded oligonucleotide associated with the first barcoded oligonucleotide in the combined nucleic acid, and determining the location of a second feature in the second array.

Analysis of Chromatin Using a Nicking Enzyme

Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.

Methods for determining a location of a biological analyte in a biological sample

Provided herein are methods of determining a location of a biological analyte in a biological sample.

Asynchronous magnetic bead rotation (AMBR) microviscometer for analysis of analytes

The disclosure provides a label-free viscosity-based analyte detection system using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. It is disclosed herein that the bead rotation period is linearly proportional to the viscosity of a solution comprising analytes surrounding the paramagnetic bead. Optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of analyte concentration. The results demonstrate the feasibility of viscosity-based analyte detection using AMBR in microscale aqueous volumes.

Convective pcr device

DNA sequencing using hydrogel beads

Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.

Multiplex nucleic acid detection methods and systems

A method of quantifying multiple target nucleic acid sequences in a sample includes generating from a sample at least a plurality of first template molecules and at least a plurality of second template molecules. At least part of said first and at least part of said second template molecules are randomly distributed into individual reaction sites. A cluster of nucleic acid amplicons of said first template molecule and at least a cluster of nucleic acid amplicons of said second template molecule are generated by clonal amplification or replication. The ID codes of all said nucleic acid amplicon clusters are identified. The quantity of at least said first and second target nucleic acid sequences in said sample is quantified by statistical analysis of respective positive numbers of identified unique ID codes of first and second target nucleic acid sequences.

Multiplex nucleic acid detection methods and systems

The present invention relates to methods and systems for single molecule based nucleic acid amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.

METHODS FOR SAMPLE USE

PREPARAÇÃO DE AMOSTRA PARA TIPOS DIFÍCEIS DE AMOSTRA. Trata-se de dispositivos e métodos para coletar e manusear tipos difíceis de amostra. Em um aspecto da presente descrição, um método de coleta de amostra é fornecido, sendo que o método compreende coletar uma amostra que compreende uma substância biológica ou ambiental através do uso de um swab, dispor o swab com a amostra coletada em um tampão de amostra e filtrar a amostra. Em várias modalidades ilustrativas, o tampão de amostra pode incluir uma protease ou um detergente e a amostra pode ser coletada e transferida para o tampão de amostra através do uso de um swab flocado. SAMPLE PREPARATION FOR DIFFICULT SAMPLE TYPES. These are devices and methods for collecting and handling difficult types of samples. In one aspect of the present description, a sample collection method is provided, the method comprising collecting a sample comprising a biological or environmental substance through the use of a swab, disposing the swab with the collected sample in a sample buffer and filter the sample. In various illustrative embodiments, the sample buffer may include a protease or a detergent and the sample may be collected and transferred to the sample buffer using a flocked swab.

Use of liquid chromatography and mass spectrometry to characterize oligonucleotides

The disclosure provides methods of characterizing a sample of oligonucleotides of interest using liquid chromatography and mass spectrometry.

Sample preparation of difficult sample types

提供用于收集和处理困难样品类型的装置和方法。在本公开的一个方面，提供样品收集方法，所述方法包括使用拭子收集包含生物物质或环境物质的样品、将具有收集的样品的所述拭子置于样品缓冲液中和过滤所述样品。在各个说明性实施方案中，样品缓冲液可包含蛋白酶或洗涤剂，并且所述样品可使用植绒拭子收集并转移至所述样品缓冲液。

Method for real-time nucleic acid amplification by droplet manipulation

The present invention provides a real time nucleic acid amplification reaction method comprising performing a nucleic acid amplification reaction in a droplet present in a container. The droplet is composed of a nucleic acid amplification reaction liquid including a nucleic acid to be amplified and magnetic particles. The container holds a droplet encapsulating medium immiscible with the nucleic acid amplification reaction liquid forming the droplet, and has a transport surface having a temperature gradient. Fluorochrome is initially contained in the droplet encapsulating medium, and optionally in the droplet, at start of the nucleic acid amplification reaction. The droplet is transported together with the magnetic particles by generating and applying a magnetic field so that the droplet is placed on the transport surface at a temperature point at which the nucleic acid synthesis reaction is started and maintained, thereby controlling a temperature of the reaction liquid.

Synthesis of DNA

本发明涉及用于合成DNA、RNA、蛋白质及类似分子的改进的方法，尤其是DNA的无细胞酶促合成，优选以大的规模。本发明涉及使用链置换复制的DNA合成，且控制向反应混合物添加核苷酸，从而控制产率。反应混合物含有起始量的核苷酸、聚合酶和DNA模板，以受控的方式向其供给另外的核苷酸。

Methods of using master / copy arrays for spatial detection

This disclosure provides methods for spatial profiling of biological analytes present in a biological sample. Methods include generating feature arrays using a master/copy format using recessed arrays, and methods for using such arrays. For example spatially-tagged analyte capture analytes can be used in spatial detection in methods to determine the location of analytes (e.g., proteins) in biological samples.

Square wave thermal cycling

Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Imaging system hardware

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

Sample preparation of difficult sample types

提供用于收集和处理困难样品类型的装置和方法。在本公开的一个方面，提供样品收集方法，所述方法包括使用拭子收集包含生物物质或环境物质的样品、将具有收集的样品的所述拭子置于样品缓冲液中和过滤所述样品。在各个说明性实施方案中，样品缓冲液可包含蛋白酶或洗涤剂，并且所述样品可使用植绒拭子收集并转移至所述样品缓冲液。

Methods and compositions for loading of polymerase complexes

The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions. In particular, the described methods, compositions, and systems utilize density differentials and/or additives to increase efficiency in the distribution of polymerase compositions to a surface as compared to methods utilizing only diffusion control.

Synthesis of dna

Dna synthesis

FIELD: biotechnology. SUBSTANCE: invention refers to biotechnology and molecular biology. Disclosed is a method for cell-free DNA synthesis involving contacting a DNA template with at least one polymerase functioning on a chain substitution principle, in the presence of nucleotides and cations of one or more metals to form a reaction mixture, where isothermal amplification of the DNA matrix takes place through replication with chain substitution, wherein additional nucleotides are fed into the reaction mixture continuously or at intervals during DNA synthesis and additionally fed metal cations, such that the ratio of metal cations: nucleotides in the reaction mixture is maintained between 9:1 and 1:9. EFFECT: method enables improving DNA output and rate of synthesis thereof, which can be used in medicine and biotechnology. 26 cl, 13 dwg, 7 tbl, 5 ex

Generating spatial arrays with gradients

The present disclosure relates to materials and methods for generating spatial arrays with gradients and using the generated spatial arrays to identify the location of analytes present in a biological sample.

Sample preparation for difficult sample types

Patent RU2017111097A3

Use of liquid chromatography and mass spectrometry to characterize oligonucleotides

The disclosure provides methods of characterizing a sample of oligonucleotides of interest using liquid chromatography and mass spectrometry.

Square wave thermal cycling

Sample preparation for difficult sample types

Devices and methods are provided for collecting and handling difficult sample types. In one aspect of the present disclosure, a sample collection method is provided, the method comprising collecting a sample comprising a biological or environmental substance using a swab, placing the swab with the collected sample in a sample buffer, and filtering the sample. In various illustrative embodiments, the sample buffer may include a protease or a detergent and the sample may be collected and transferred to the sample buffer using a flocked swab.

Counteracting osmotic imbalance in a sequencing cell

A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.

Uso de cromatografía líquida y espectrometría de masas para caracterizar oligonucleótidos.

La descripción proporciona métodos para caracterizar una muestra de oligonucleótidos de interés mediante el uso de cromatografía líquida y espectrometría de masas.

DNA:n synteesi

Imaging system hardware

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

Mobile solid phase compositions for use in biochemical reactions and analyses

Compositions that include particle suspensions where such particle suspensions have characteristics for use in a variety of applications including, for example, flow restriction, reagent delivery, and use in microfluidic systems. In some compositions provided, the particle suspension include deformable particles and in particular compositions the deformable particles are beads or gel beads.

Nucleic acid capture, concentration, and purification

An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.

Analysis of chromatin using a nicking enzyme

Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.

Method of amplifying nucleic acids

核酸の捕捉、濃縮、及び精製

キットの一例には、フローセルアセンブリが含まれる。フローセルアセンブリは、反応チャンバと、反応チャンバの入口と選択的に流体連通する温度制御流路と、温度制御流路内に配置されたフィルタと、を含む。反応チャンバは、間隙領域によって分離された凹部と、凹部の各々の中に付着した捕捉プライマーと、を含む。フィルタは、ｉ）第１の温度で温度制御流路内で生成された濃縮生体試料－ポリマー複合体を遮断し、ｉｉ）第２の温度で温度制御流路内の複合体から放出された濃縮生体試料及びポリマーの通過を可能にする。

Synthesis of dna

Analysis of chromatin using a nicking enzyme

Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.

Dnr sintezė

Dna-syntese

Analysis of Chromatin Using a Nicking Enzyme

Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.

Nucleic acid capture, concentration, and purification

An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.

Nucleic acid capture, concentration, and purification

An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.

核酸多联体及其稳定和/或压缩方法

本公开在一些方面涉及用于准确检测和定量生物样品中存在的多种分析物的方法和组合物。在一些方面，本文提供的所述方法和组合物解决了一个或多个与所述生物样品中核酸结构如RCP的稳定性和/或大小相关的问题，而无需使用外源添加的寡核苷酸压缩探针。在一些实施方案中，本文提供了涉及使用自杂交性杂交区来压缩和/或稳定核酸多联体(例如，RCP)的方法。在一些实施方案中，RCP的串联单元之间的动态链间退火用于压缩和/或稳定。在一些实施方案中，RCP中的短回文区用于压缩和/或稳定。

Method of amplifying nucleic acids

核酸捕获、浓缩和纯化

一种试剂盒的示例包括流动池组件。该流动池组件包括反应室、与该反应室的入口选择性流体连通的温控流动通道和定位在该温控流动通道中的过滤器。该反应室包括由间隙区域分开的凹入部和附接在该等凹入部中的每一个内的捕获引物。该过滤器i)在第一温度下阻挡在该温控流动通道中产生的浓缩的生物样品‑聚合物复合物，以及ii)在第二温度下允许该温控流动通道中从该等复合物中释放的浓缩的生物样品和聚合物通过。

Captura, concentração e purificação de ácido nucleico

CAPTURA, CONCENTRAÇÃO E PURIFICAÇÃO DE ÁCIDO NUCLEICO. A presente invenção se refere a um exemplo de um kit que inclui um conjunto de células de fluxo. O conjunto de células de fluxo inclui uma câmara de reação, um canal de fluxo com temperatura controlada em comunicação fluida seletiva com uma entrada da câmara de reação e um filtro posicionado no canal de fluxo com temperatura controlada. A câmara de reação inclui depressões separadas por regiões intersticiais e primers de captura fixados dentro de cada uma das depressões. O filtro é i) para bloquear complexos de amostra biológica concentrada-polímero gerados no canal de fluxo com temperatura controlada a uma primeira temperatura e ii) para permitir a passagem de amostra biológica concentrada e polímero liberados dos complexos no canal de fluxo com temperatura controlada a uma segunda temperatura.

Captura, concentración y purificación de ácido nucleico.

Un ejemplo de un kit incluye una unidad de celda de flujo. La unidad de celda de flujo incluye una cámara de reacción, un canal de flujo controlado por temperatura en comunicación fluida selectiva con una entrada de la cámara de reacción, y un filtro posicionado en el canal de flujo controlado por temperatura. La cámara de reacción incluye depresiones separadas por regiones intersticiales y cebadores de captura unidos dentro de cada una de las depresiones. El filtro es i) para bloquear los complejos de polímeros de muestra biológica concentrados generados en el canal de flujo controlado por temperatura a una primera temperatura, y ii) para permitir el paso de la muestra biológica concentrada y el polímero liberado de los complejos en el canal de flujo controlado por temperatura a una segunda temperatura.

Nucleic acid capture, concentration, and purification

An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.

Nucleic acid capture, concentration, and purification

An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.

Nucleic acid concatemers and methods for stabilizing and/or compacting the same

The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures, such as RCPs, in the biological sample without the use of exogenously added oligonucleotide compaction probes. In some embodiments, provided herein are methods involving the use of self-hybridizing hybridizing regions for compacting and/or stabilizing nucleic acid concatemers (e.g., RCPs). In some embodiments, dynamic inter-strand annealing between tandem units of an RCP is used for compaction and/or stabilization. In some embodiments, short palindromic regions in an RCP are used for compaction and/or stabilization.

Imaging system hardware

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

칸몬흰개미 판별용 프라이머 조성물 및 이를 포함하는 진단키트

본 발명은 칸몬흰개미(Reticulitermes kanmonensis) 판별용 프라이머 조성물 및 이를 포함하는 진단키트에 관한 것으로서, 보다 상세하게는 국내외 흰개미류로부터 칸몬흰개미를 신속하고 정확하게 판별할 수 있는 칸몬흰개미 판별용 프라이머 조성물 및 이를 포함하는 진단키트에 관한 것이다.

Clearance buffer

The invention concerns a composition comprising TCEP, biotin and dextran suitable for liquefying a viscous biological sample. The composition according to the invention can be used in diagnostic methods, preferably for use in diagnosis of an infection with a micro-organism, more preferably for use in diagnosis of HCAP.

흰개미 판별용 프라이머 조성물 및 이를 포함하는 진단키트

본 발명은 집흰개미(Reticulitermes speratus) 판별용 프라이머 조성물 및 이를 포함하는 진단키트에 관한 것으로서, 보다 상세하게는 국내외 흰개미류로부터 집흰개미를 신속하고 정확하게 판별할 수 있는 집흰개미 판별용 프라이머 조성물 및 이를 포함하는 진단키트에 관한 것이다.

Imaging system hardware

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

핵산 포획, 농도 및 정제

키트의 예는 플로우 셀 어셈블리를 포함한다. 플로우 셀 어셈블리는 반응 챔버, 반응 챔버의 입구와 선택적으로 유체 연통하는 온도 제어식 플로우 채널, 및 온도 제어식 플로우 채널 내에 위치되는 필터를 포함한다. 반응 챔버는 간극 영역에 의해 분리된 함몰부 및 각각의 함몰부 내에 부착된 포획 프라이머를 포함한다. 필터는 i) 제1 온도에서 온도 제어식 플로우 채널 내에서 생성된 농축된 생물학적 샘플-중합체 복합체를 차단하고, ii) 제2 온도에서 온도 제어식 플로우 채널 내에서 복합체로부터 방출된 농축된 생물학적 샘플 및 중합체의 통과를 허용하기 위한 것이다.

核酸多联体及其稳定和/或压缩方法

本公开在一些方面涉及用于准确检测和定量生物样品中存在的多种分析物的方法和组合物。在一些方面，本文提供的所述方法和组合物解决了一个或多个与所述生物样品中核酸结构如RCP的稳定性和/或大小相关的问题，而无需使用外源添加的寡核苷酸压缩探针。在一些实施方案中，本文提供了涉及使用自杂交性杂交区来压缩和/或稳定核酸多联体(例如，RCP)的方法。在一些实施方案中，RCP的串联单元之间的动态链间退火用于压缩和/或稳定。在一些实施方案中，RCP中的短回文区用于压缩和/或稳定。

Nucleic acid capture, concentration, and purification

An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.

核酸捕获、浓缩和纯化

一种试剂盒的示例包括流动池组件。该流动池组件包括反应室、与该反应室的入口选择性流体连通的温控流动通道和定位在该温控流动通道中的过滤器。该反应室包括由间隙区域分开的凹入部和附接在该等凹入部中的每一个内的捕获引物。该过滤器i)在第一温度下阻挡在该温控流动通道中产生的浓缩的生物样品‑聚合物复合物，以及ii)在第二温度下允许该温控流动通道中从该等复合物中释放的浓缩的生物样品和聚合物通过。

液相色谱法和质谱法用于表征寡核苷酸的用途

本公开提供了使用液相色谱法和质谱法来表征所关注寡核苷酸的样品的方法。

困难样品类型的样品制备

提供用于收集和处理困难样品类型的装置和方法。在本公开的一个方面，提供样品收集方法，所述方法包括使用拭子收集包含生物物质或环境物质的样品、将具有收集的样品的所述拭子置于样品缓冲液中和过滤所述样品。在各个说明性实施方案中，样品缓冲液可包含蛋白酶或洗涤剂，并且所述样品可使用植绒拭子收集并转移至所述样品缓冲液。

Synthesis of dna

The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner.

凝胶前驱体、核酸扩增试剂凝胶、存储芯片和使用方法

本发明公开了一种凝胶前驱体、核酸扩增试剂凝胶、存储芯片和使用方法，以改善现有技术在对核酸扩增所需使用的试剂存储运输时，存在保存效果不佳，试剂性能降低的问题。所述凝胶前驱体，包括：海藻酸钠主体溶液，以及混合于所述海藻酸钠主体溶液中的以下至少一种成分：淀粉；糊精；壳聚糖；琼脂糖；明胶，其中，所述海藻酸钠主体溶液的质量浓度为0.01％w/v～5％w/v。

成像系统硬件

一种样品保持器，其包括具有第一保持机构的第一构件，所述第一保持机构被配置为保持包括样品的第一基材，具有第二保持机构的第二构件，所述第二保持机构被设置为保持包括试剂介质的第二基材，以及对准机构，所述对准机构连接至第一和第二构件中至少一者，并且设置为将第一和第二构件对准，从而使得在第一和第二构件对准时，样品接触试剂介质的至少部分。

生成用于空间分析的捕获探针

本公开涉及用于在基材上产生用于识别分析物在生物样品中的位置的捕获探针的组合物和方法。

使用主/复制阵列进行空间检测的方法

本公开提供了对生物样品中存在的生物分析物进行空间概况分析的方法。方法包括使用凹陷阵列，利用主/复制形式来生成特征阵列，以及使用此类阵列的方法。例如，空间加标的分析物捕获分析物可用于确定生物样品中分析物(例如，蛋白质)位置的方法中的空间检测。

Use of liquid chromatography and mass spectrometry to characterize oligonucleotides

The disclosure provides methods of characterizing a sample of oligonucleotides of interest using liquid chromatography and mass spectrometry.

Síntesis del ADN

La presente invención se refiere a un proceso mejorado para la síntesis de ADN, ARN, proteínas y moléculas similares, en particular la síntesis enzimática de ADN sin células, preferiblemente a gran escala. La presente invención se refiere a la síntesis de ADN mediante replicación por desplazamiento de cadena y se controla la adición de nucleótidos a la mezcla de reacción, controlando así el rendimiento. La mezcla de reacción contiene una cantidad inicial de nucleótidos, polimerasa y molde de ADN, a los que se suministran más nucleótidos de forma controlada. (Traducción automática con Google Translate, sin valor legal)

Gel precursor, nucleic acid amplification reagent gel, storage chip and using method thereof

The present disclosure can provide a gel precursor, a nucleic acid amplification reagent gel, a storage chip and a using method thereof. The gel precursor includes: a sodium alginate main solution mixed with at least one of the following components: starch, dextrin, chitosan, agarose and gelatin, where the sodium alginate main solution has a mass concentration range from 0.01% w/v to 5% w/v.

通过试剂容器以热对流进行定量聚合酶链式反应的装置

本发明提供一种通过试剂容器以热对流进行定量聚合酶链式反应的装置，包括：第一架体，设于水平面且具有第一穿孔，风扇与通风口，具有上表面及下表面，下表面包含加热线圈及第一温度感测器；第二架体，设于第一架体的下方与水平面平行，具有第二穿孔，上表面及下表面，下表面包含与水平面平行的夹持凹槽且第二穿孔与夹持凹槽连接；玻璃装置，设于夹持凹槽内，包含上平面、下平面及接点，上平面或下平面具有透明导电薄膜，玻璃的大小与夹持凹槽相同并以上平面或下平面固定于夹持凹槽，接点设于涂布有透明导电薄膜的同一侧；电源供应装置；光源；光信号接收器；处理器；以及容置空间，设于第一架体及第二架体间。本发明可节省整体反应时间。

Microorganism nucleic acid purification from host samples

The present disclosure provides systems, devices, and methods for purifying microorganism nucleic acid from a host sample, such as a whole blood sample from a human. In certain embodiments, devices and systems with multiple filters are employed and provide for the selective removal of blood cells and host nucleic acids from a sample in order to enrich for microorganism nucleic acid.

Filtering small nucleic acids using permeabilized cells

Filtering small nucleic acids using permeabilized cells and methods for using the filtering to detect genomic DNA accessibility are described.

High efficiency multiplexed nucleic acid capture in a structured microenvironment

Provided herein is a method for sample analysis. In some embodiments, the method may involve: a) enzymatically attaching a reactive group to nucleic acid molecules in a sample; b) covalently reacting the reactive group with surface exposed reactive sites on a porous support, thereby covalently tethering the nucleic acid molecules to the porous support; c) performing a primer extension reaction using the tethered nucleic acid molecules as a template to produce primer extension products; and d) eluting the primer extension products from the porous support, while leaving the tethered nucleic acid molecules tethered to the porous support.

Surface energy gradient films for analytical devices

A device comprising a channel comprising at least one surface wherein the surface comprises a fluid-impervious base surface covered by a coating beginning at a proximal location and ending at a distal location along the at least one surface of the channel, the coating forming a surface energy gradient from the proximal location to the distal location on the surface, wherein the coating comprises a species having a functional group M1 and a species having a functional group M2 where M1 and M2 have different surface energies, wherein the functional group M2 comprises an amide or amine group, and the concentration of the species comprising functional group M2 in the coating increases relative to the concentration of the species comprising functional group M1 from the proximal location to the distal location on the surface.