DETERGENT COMPOSITION COMPRISING FATTY ACID PROCESSING ENZYMES

A liquid detergent composition, preferably a liquid manual dishwashing detergent composition, comprising one or more hydroperoxy fatty acid producing enzymes selected from the group consisting of: arachidonate lipoxygenases, alpha-dioxygenases, and mixtures thereof, preferably alpha-dioxygenases, a surfactant system and a liquid carrier (i.e., water). Methods of washing comprising the liquid detergent composition are also provided.

Epoxidation using peroxygenase

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alkene, or a terpene.

Process for the purification of vanadium bromoperoxidase

A process for the purification of vanadium bromoperoxidase from a variety of sources is disclosed. This simple process provides highly pure enzyme for a variety of uses including in analytical assays.

Enzymatic Hydroxylation of Aliphatic Hydrocarbon

The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

DETERGENT COMPOSITION

A detergent composition, preferably a manual dishwashing detergent composition, comprising one or more diol synthases capable of converting one or more unsaturated fatty acids into one or more oxylipins, and a surfactant system comprising one or more anionic surfactants and one or more co-surfactants selected from the group consisting of amphoteric surfactant, zwitterionic surfactant, and mixtures thereof. Method of using the detergent composition comprising a surfactant system and the diol synthases are also provided.

Polypeptides having haloperoxidase activity

NUCLEIC ACIDS ENCODING POLYPEPTIDES HAVING HALOPEROXIDASE ACTIVITY

The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.

ENZYMATIC HYDROXYLATION OF ALIPHATIC HYDROCARBON

POLYPEPTIDES HAVING HALOPEROXIDASE ACTIVITY

The present invention relates to isolated polypeptides having haloperoxidase activity. The invention also relates to methods for producing and using the polypeptides ...

EPOXIDATION USING PEROXYGENASE

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alkene, or a terpene.

Expoxidation using peroxygenases

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alken, or a terpen.

Epoxidation using peroxygenase

The invention relates to enzymatic methods for epoxidation of a non-cyclicaliphatic alken, or a terpen.

PROCESS FOR THE PURIFICATION OF VANADIUM BROMOPEROXIDASE

A process for the purification of vanadium bromoperoxidase from a variety of sources is disclosed. This simple process provides highly pure enzyme for a variety of uses including in analytical assays.

EPOXIDATION USING PEROXYGENASE

The invention relates to enzymatic methods for epoxidation of a non-cyclicaliphatic alken, or a terpen.

ENZYMATIC HYDROXYLATION OF ALIPHATIC HYDROCARBON

The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

EPOXIDATION USING PEROXYGENASE

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alkene, or a terpene.

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

ENZYMATIC HYDROXYLATION OF ALIPHATIC HYDROCARBON

The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

With peroxidase activity of the polypeptide and polynucleotide encoding the polypeptide

SYNTHESIS OF FUNCTIONAL UNSPECIFIC PEROXIDASES USING A NON-FUNGAL EUKARYOTIC CELL-FREE SYSTEM

The invention relates to a method for preparing a soluble and functional peroxygenase in cell-free systems which comprises at least the following steps: a) providing a reaction medium comprising at least the following components: a nucleic acid template coding for a fungal unspecific peroxygenase (UPO) classified as EC 111.2.1, an RNApolymerase, a eukaryotic cell lysate derived from insect cells or mammalian cells and supplemented with glutathione and glutathione-disulfide during lysate preparation wherein the cell lysate further comprises non-fungal membrane vesicles, as well as heme or a heme derivative, in particular a heme complex selected from the group comprising or consisting of hematin and hemin; b) performing a cell-free reaction to synthesize the peroxygenase protein. The invention further relates to a method for screening and characterization of mutants or variants of unspecific peroxygenases classified as EC 1.11.2.1., which method comprises steps a)- and b) of the method above ...

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

DETERGENT COMPOSITION

A detergent composition, preferably a manual dishwashing detergent composition, including one or more diol synthases capable of converting one or more unsaturated fatty acids into one or more oxylipins, and a surfactant system including one or more anionic surfactants and one or more co-surfactants selected from the group consisting of amphoteric surfactant, zwitterionic surfactant, and mixtures thereof. Method of using the detergent composition including a surfactant system and the diol synthases are also provided.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Nucleic acids encoding polypeptides having haloperoxidase activity

Polypeptides having haloperoxidase activity

The present invention relates to isolated polypeptides having haloperoxidase activity. The invention also relates to methods for producing and using the polypeptides ...

Polypeptides Having Peroxygenase Activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 143-. (canceled)44. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted , linear or branched , alkane or saturated fatty acid having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 , comprising contacting the alkane or saturated fatty acid with hydrogen peroxide and a polypeptide having peroxygenase activity and at least 95% sequence identity to the sequence of amino acids 1 to 244 of SEQ ID NO: 2 , wherein the alkane or saturated fatty acid is optionally substituted with one or two substituents selected from the group consisting of halogen , hydroxyl , carboxyl , amino , nitro , cyano , thiol , sulphonyl , formyl , acetyl , methoxy , ethoxy , phenyl , benzyl , xylyl , carbamoyl and sulfamoyl.45. The method of claim 44 , wherein an alkane is hydroxylated.46. The method of claim 45 , wherein the alkane is pentane claim 45 , hexane claim 45 , heptane claim 45 , octane claim 45 , nonane claim 45 , decane claim 45 , undecane claim 45 , dodecane claim 45 , tridecane claim 45 , tetradecane claim 45 , pentadecane or hexadecane claim 45 , or an isomer thereof.47. The method of claim 45 , wherein the alkane is unsubstituted.48. The method of claim 45 , wherein the alkane is linear.49. The method of claim 45 , wherein the alkane is converted to a diol by introduction of two hydroxy groups.50. The method of claim 44 , wherein a saturated fatty acid is hydroxylated.51. The method of claim 50 , wherein the saturated fatty acid is selected from the group consisting of butanoic acid claim 50 , pentanoic acid claim 50 , hexanoic acid claim 50 , heptanoic acid claim 50 , octanoic acid claim 50 , nonanoic acid ...

Detergent composition

A liquid detergent composition, preferably a liquid manual dishwashing detergent composition, comprising one or more hydroperoxy fatty acid producing enzymes selected from the group consisting of: arachidonate lipoxygenases, alpha-dioxygenases, and mixtures thereof, preferably alpha-dioxygenases, a surfactant system and a liquid carrier (i.e., water). Methods of washing comprising the liquid detergent composition are also provided.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Thielavia terrestris.

Epoxidation using peroxygenase

The invention relates to enzymatic methods for epoxidation of a non-cyclicaliphatic alken, or a terpen.

Verfahren zur enzymatischen Herstellung von zytostatischen 4-Hydroxy-Oxazaphosphorinen

Die vorliegende Erfindung betrifft ein enzymatisches Verfahren zur Herstellung von zytostatischen 4-Hydroxy-Oxazaphosphorinen.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Thielavia hyrcaniae.

POLYPEPTIDES HAVING HALOPEROXIDASE ACTIVITY

The present invention relates to isolated polypeptides having haloperoxidase activity more than 50% residual activity, after 15 minutes incubation at 70° and pH 7. The invention also relates to methods for producing and using the polypeptides ...

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

NUCLEIC ACIDS ENCODING POLYPEPTIDES HAVING HALOPEROXIDASE ACTIVITY

The present invention relates to isolated nucleic acid sequences encoding polypeptides having more than 50 % residual haloperoxidase activity after 15 minutes incubation at 70 °C and pH7. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.

UNSPECIFIC PEROXYGENASE ENZYME VARIANTS FOR SELECTIVE FATTY ACID EPOXIDATION OR HYDROXYLATION

The invention relates to a recombinant Marasmius rotula unspecific peroxygenase (rMroURO) and two mutants thereof, wherein said mutants show enhanced selectivity towards either the epoxidation or the (sub)terminal ω/(ω-1)-hydroxylation of unsaturated fatty acids. The invention also refers to the use of these enzyme variants for the specific epoxidation or hydroxylation of fatty acids such as oleic acid, linoleic acid and/or alpha-linolenic acid.

PROCESS FOR THE ENHANCEMENT OF THE BIODEGRADABILITY OF POLYOLEFINIC MATERIALS

Peroxygenase Variants

The present invention relates to peroxygenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. 143-. (canceled)44. A peroxygenase variant , comprising a substitution at one or more positions corresponding to positions E16 , Y18 , P28 , P31 , W38 , R50 , Y72 , F92 , M94 , I98 , T100 , H102 , C103 , Y104 , H107 , L114 , A144 , F146 , M152 , F163 , Q167 , Y180 , T188 , W195 , M202 , Y215 , F216 , S222 , F224 , F228 , F229 , M246 , F253 , H256 , Y258 , Y281 , Y287 , F308 , L317 , T319 , S345 , F346 , P347 , G348 , S349 , and G350; preferably M94 , A144 , Y180 , W195 , Y215 , F224 , F228 , and F229 of the mature polypeptide of SEQ ID NO: 2 , wherein the variant has peroxygenase activity and has at least 60% but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 2.45. The variant of claim 44 , which has at least 65% sequence identity to the mature polypeptide of SEQ ID NO: 2.46. The variant of claim 44 , which has at least 70% sequence identity to the mature polypeptide of SEQ ID NO: 2.47. The variant of claim 44 , which has at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2.48. The variant of claim 44 , which has at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2.49. The variant of claim 44 , which has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2.50. The variant of claim 44 , which has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.51. A polynucleotide encoding the variant of .52. A nucleic acid construct or expression vector comprising the polynucleotide of .53. A host cell comprising the polynucleotide of .54. A method of producing a peroxygenase variant claim 51 , comprising:{'claim-ref': {'@idref': 'CLM-00053', 'claim 53'}, '(a) cultivating the host cell of under conditions suitable for expression ...

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Epoxidation using peroxygenase

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alkene, or a terpene.

Peroxygenase variants

The present invention relates to peroxygenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

DETERGENT COMPOSITION

A detergent composition, preferably a manual dishwashing detergent composition, including one or more diol synthases capable of converting one or more unsaturated fatty acids into one or more oxylipins, and a surfactant system including one or more anionic surfactants and one or more co-surfactants selected from the group consisting of amphoteric surfactant, zwitterionic surfactant, and mixtures thereof. Method of using the detergent composition including a surfactant system and the diol synthases are also provided.

Process for the purification of vanadium bromoperoxidase

A process for the purification of vanadium bromoperoxidase from a variety of sources is disclosed. This simple process provides highly pure enzyme for a variety of uses including in analytical assays.

DETERGENT COMPOSITION

A surfactant containing detergent composition, preferably a manual dishwashing detergent composition, comprising either a combination of one or more hydroperoxy fatty acid producing enzymes and one or more hydroperoxy fatty acid converting enzymes, or at least one fatty acid processing fusion enzyme comprising at least two catalytic domains: a hydroperoxy fatty acid producing domain and a hydroperoxy fatty acid converting, and methods of using such compositions.

RECOMBINANT HEME THIOLATE OXYGENASES

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Daldinia caldariorum.

Polypeptides having haloperoxidase activity

The present invention relates to isolated polypeptides having haloperoxidase activity. The invention also relates to methods for producing and using the polypeptides ...

Verfahren zur selektiven Oxygenierung von Testosteron und Testosteron-ähnlichen Steroiden

Gegenstand der Erfindung ist ein Verfahren zur enzymatischen selektiven Einführung von Sauerstoff in das Grundgerüst von vorzugsweise Testosteron und Testosteron-ähnlichen Steroiden in einem wasserhaltigen Lösungsmittel in Gegenwart einer Peroxygenase (EC 1.11.2.1), vorzugsweise aus einem Ascomyceten, unter Erhalten eines 16α-Hydroxids des 3-on 4-en Sterangrundgerüstes und eines Epoxids am 4-en des Sterangrundgerüstes. Die Erfindung ermöglicht vorteilhaft die Synthese schwerzugänglicher hydroxylierter Steroidstrukturen.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

PROCESS FOR THE ENHANCEMENT OF THE BIODEGRADABILITY OF POLYOLEFINIC MATERIALS

A process for enhancing biodegradability of polyolefinic materials is provided. The process includes providing a polyolefinic material, mixing the polyolefinic material with at least one fatty reagent, heating up the polyolefinic material mixed with the at least one fatty reagent to the melting temperature of the polyolefinic material to obtain a melted material, letting the melted material cool at room temperature for a time sufficient to obtain a solidified product, and incubating the solidified product with at least one fungal mycelium selected from fungal strains secreting Unspecific Peroxygenases (UPO), in presence of a fungal culture medium.

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Thielavia terrestris.

MUTANTS OF UNSPECIFIC PEROXYGENASE WITH HIGH MONOOXYGENASE ACTIVITY AND USES THEREOF

The invention relates to an unspecific peroxygenase of the Agrocybe aegerita fungus, obtained by means of directed molecular evolution to facilitate the functional expression thereof in an active, soluble and stable form. The peroxygenase described in the invention shows a significant improvement in the functional expression thereof, improved monooxygenase activity and reduced peroxidase activity, in relation to the monooxygenase and peroxidase activities showed by the unspecific wild-type peroxygenase of A. aegerita . The peroxygenase of the invention is useful in chemical processes, including industrial transformations such as the selective oxyfunctionalisation of carbon-hydrogen bonds of various organic compounds.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 143-. (canceled)44. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted , linear or branched , aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 , comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide having peroxygenase activity , wherein the polypeptide has at least 80% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.45. A method for hydroxylation in position 2 or 3 of the terminal end of an acyl group of a lipid , comprising contacting the lipid with hydrogen peroxide and a polypeptide having peroxygenase activity , wherein the polypeptide has at least 80% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.46. A method for introducing a hydroxy or a keto group at the second or third carbon of at least two ends of a substituted or unsubstituted , linear or branched , aliphatic hydrocarbon having at least five carbons and having at least one hydrogen attached to said second or third carbon , comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide having peroxygenase activity , wherein the polypeptide has at least 80% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.47. The method of claim 44 , wherein the aliphatic hydrocarbon is an alkane.48. The method of claim 45 , wherein the aliphatic hydrocarbon is an alkane.49. The method of claim 46 , wherein the aliphatic hydrocarbon is an alkane.50. The method of claim 47 , wherein the alkane is pentane claim 47 , hexane claim 47 , heptane claim 47 , octane claim 47 , nonane claim 47 ...

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

EPOXIDATION USING PEROXYGENASE

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alkene, or a terpene. 115-. (canceled)16. A method for producing an epoxide , comprising contacting a non-cyclic aliphatic alkene or a terpene with hydrogen peroxide and a peroxygenase (EC 1.11.2.1) , wherein the peroxygenase comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 16.17. The method of claim 16 , wherein the peroxygenase comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 16.18. The method of claim 16 , wherein the peroxygenase comprises an amino acid sequence having at least 97% sequence identity to SEQ ID NO: 16.19. The method of claim 16 , wherein the peroxygenase comprises an amino acid sequence having at least 98% sequence identity to SEQ ID NO: 16.20. The method of claim 16 , wherein the peroxygenase comprises an amino acid sequence having at least 99% sequence identity to SEQ ID NO: 16.21. The method of claim 16 , wherein the peroxygenase comprises an amino acid sequence having the amino acid sequence of SEQ ID NO: 16.22. The method of claim 16 , wherein the amino acid sequence comprises the motif E-H-D-[G claim 16 ,A]-S-[L claim 16 ,I]-S-R (SEQ ID NO: 21).23. The method of claim 16 , wherein the aliphatic alkene has one or more substituents selected from the group consisting of halogen claim 16 , hydroxyl claim 16 , carboxyl claim 16 , amino claim 16 , nitro claim 16 , cyano claim 16 , thiol claim 16 , sulphonyl claim 16 , formyl claim 16 , acetyl claim 16 , methoxy claim 16 , ethoxy claim 16 , carbamoyl and sulfamoyl.24. The method of claim 23 , wherein the substituent(s) are selected from the group consisting of chloro claim 23 , hydroxyl claim 23 , carboxyl and sulphonyl.25. The method of claim 16 , wherein the aliphatic alkene consists of at least three carbons claim 16 , and has a carbon-carbon double bond at one end.26. The method of claim 16 , wherein the aliphatic alkene is propene claim 16 ...

Peroxygenase variants

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Pestalotiopsis virgatula.

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 129-. (canceled)30. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted , linear or branched , aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 , comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide having peroxygenase activity , wherein the polypeptide has at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2 or is a fragment of the mature polypeptide of SEQ ID NO: 2 and wherein the mature polypeptide is amino acids 1 to 261 of SEQ ID NO: 2.31. The method of claim 30 , wherein the aliphatic hydrocarbon is an alkane.32. The method of claim 31 , wherein the alkane is pentane claim 31 , hexane claim 31 , heptane claim 31 , octane claim 31 , nonane claim 31 , decane claim 31 , undecane claim 31 , dodecane claim 31 , tridecane claim 31 , tetradecane claim 31 , pentadecane or hexadecane claim 31 , or isomers thereof.33. The method of claim 30 , wherein the aliphatic hydrocarbon is unsubstituted.34. The method of claim 30 , wherein the aliphatic hydrocarbon is linear.35. The method of claim 30 , wherein the aliphatic hydrocarbon is converted to a diol claim 30 , by introduction of two hydroxy groups.36. The method of claim 30 , wherein the aliphatic hydrocarbon is a fatty acid.37. The method of claim 30 , wherein the polypeptide has at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2.38. The method of claim 30 , wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2.39. The method of claim 30 , wherein the polypeptide has at least 95% sequence identity ...

Enzymatic hydroxylation of aliphatic hydrocarbon

The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

With over cyclooxygenase activity polypeptide

Polypeptides Having Peroxygenase Activity And Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polynucleotide, host cell and a method to recombinantly produce the protein encoded by said polynucleotide having peroxygenative activity

The invention relates to an unspecific peroxygenase of the Agrocybe aegerita fungus, obtained by means of directed molecular evolution to facilitate the functional expression thereof in an active, soluble and stable form. The peroxygenase described in the invention shows a significant improvement in the functional expression thereof, improved monooxygenase activity and reduced peroxidase activity, in relation to the monooxygenase and peroxidase activities showed by the unspecific wild-type peroxygenase of A. aegerita. The peroxygenase of the invention is useful in chemical processes, including industrial transformations such as the selective oxyfunctionalisation of carbon-hydrogen bonds of various organic compounds.

Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 129-. (canceled).30. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted , linear or branched , alkane or saturated fatty acid having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 , comprising contacting the alkane or saturated fatty acid with hydrogen peroxide and a polypeptide having peroxygenase activity and at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2 , wherein the alkane or saturated fatty acid is optionally substituted with one or two substituents selected from the group consisting of halogen , hydroxyl , carboxyl , amino , nitro , cyano , thiol , sulphonyl , formyl , acetyl , methoxy , ethoxy , phenyl , benzyl , xylyl , carbamoyl and sulfamoyl.31. The method of claim 30 , wherein an alkane is hydroxylated.32. The method of claim 31 , wherein the alkane is pentane claim 31 , hexane claim 31 , heptane claim 31 , octane claim 31 , nonane claim 31 , decane claim 31 , undecane claim 31 , dodecane claim 31 , tridecane claim 31 , tetradecane claim 31 , pentadecane or hexadecane claim 31 , or an isomer thereof.33. The method of claim 31 , wherein the alkane is unsubstituted.34. The method of claim 31 , wherein the alkane is linear.35. The method of claim 31 , wherein the alkane is converted to a diol by introduction of two hydroxy groups.36. The method of claim 30 , wherein a saturated fatty acid is hydroxylated.37. The method of claim 36 , wherein the saturated fatty acid is selected from the group consisting of butanoic acid claim 36 , pentanoic acid claim 36 , hexanoic acid claim 36 , heptanoic acid claim 36 , octanoic acid claim 36 , nonanoic acid claim 36 , ...

DETERGENT COMPOSITION

A liquid detergent composition, preferably a liquid manual dishwashing detergent composition, comprising one or more hydroperoxy fatty acid producing enzymes selected from the group consisting of: arachidonate lipoxygenases, alpha-dioxygenases, and mixtures thereof, preferably alpha-dioxygenases, a surfactant system and a liquid carrier (i.e., water). Methods of washing comprising the liquid detergent composition are also provided. 1. A liquid detergent composition comprising:a) one or more hydroperoxy fatty acid producing enzymes selected from the group consisting of arachidonate lipoxygenases, alpha-dioxygenases, and mixtures thereof;b) a surfactant system comprising one or more anionic surfactants and one or more co-surfactants selected from the group consisting of amphoteric surfactant, zwitterionic surfactant, and mixtures thereof, wherein the weight ratio of the anionic surfactants to the co-surfactants is less than about 9:1; andc) from about 30 wt % to about 95 wt % by weight of the composition, of water.2. The composition according to claim 1 , wherein the one or more hydroperoxy fatty acid producing enzymes are alpha-dioxygenases.3. The composition according to claim 1 , wherein the surfactant system comprises the anionic surfactant and co-surfactant in a weight ratio of the anionic surfactants to the co-surfactants of from 4:1 to 2:1.4. The composition according to claim 1 , wherein the composition is a liquid manual dishwashing composition.5. The composition according to claim 1 , wherein the one or more hydroperoxy fatty acid producing enzymes are arachidonate lipoxygenases selected from the group consisting of arachidonate 5-lipoxygenases (EC 1.13.11.34) claim 1 , arachidonate 8-lipoxygenases (EC 1.13.11.40) claim 1 , arachidonate 12-lipoxygenases (E.C. 1.13.11.31) claim 1 , and arachidonate 15-lipoxygenase (EC 1.13.11.33).6. The composition according to claim 5 , wherein the arachidonate lipoxygenase is arachidonate 5-lipoxygenases (EC 1.13.11.34). ...

Nucleic acids encoding polypeptides having haloperoxidase activity

The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.

Expoxidation Using Peroxygenases

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alken, or a terpen.

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Epoxidation Using Peroxygenase

The invention relates to enzymatic methods for epoxidation of a non-cyclicaliphatic alken, or a terpen.

With over cyclooxygenase activity polypeptide

Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Myceliophthora fergusii.

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

PURIFICATION OF HALOPEROXIDASE

PROBLEM TO BE SOLVED: To obtain the subject enzyme high in purity, good in preservation stability and useful as a signal-forming reagent or the like for in vitro diagnosis by applying a two-phase aqueous partition chromatography to a raw material such as sea weed containing a haloperoxidase under a specific condition. SOLUTION: A homogenate prepared by homogenizing a sample including a haloperoxidase is mixed with a salt (e.g. a phosphoric acid salt or a sulfuric acid salt) and a polymer (e.g. polyethylene glycol) having about 6,000-about 20,000 dalton molecular weight to provide a mixture having pH 6.0-10.0. The concentration of the polymer in the mixture is 7-20 wt.% and the concentration of the salt is 0.6-1.7 wt.%. A polymer phase, a salt phase and an interphase zone are formed. The interphase zone is isolated, and the hydroperoxidase present in the interphase zone is solubilized and isolated in the method for purifying the haloperoxidase. A vanadium bromoperoxidase is obtained from ...

With over cyclooxygenase activity polypeptide

本发明涉及具有过氧合酶活性的分离的多肽和编码这些多肽的多核苷酸。本发明还涉及包括这些多核苷酸的核酸构建体、载体以及宿主细胞连同产生和使用这些多肽的方法。编码过氧合酶的多核苷酸分离自条纹拟盘多毛孢。

Polypeptides Having Peroxygenase Activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 143-. (canceled)44. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted , linear or branched , alkane or saturated fatty acid having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 , comprising contacting the alkane or saturated fatty acid with hydrogen peroxide and a polypeptide having peroxygenase activity and at least 95% sequence identity to the sequence of amino acids 1 to 244 of SEQ ID NO: 2 , wherein the alkane or saturated fatty acid is optionally substituted with one or two substituents selected from the group consisting of halogen , hydroxyl , carboxyl , amino , nitro , cyano , thiol , sulphonyl , formyl , acetyl , methoxy , ethoxy , phenyl , benzyl , xylyl , carbamoyl and sulfamoyl.45. The method of claim 44 , wherein an alkane is hydroxylated.46. The method of claim 45 , wherein the alkane is pentane claim 45 , hexane claim 45 , heptane claim 45 , octane claim 45 , nonane claim 45 , decane claim 45 , undecane claim 45 , dodecane claim 45 , tridecane claim 45 , tetradecane claim 45 , pentadecane or hexadecane claim 45 , or an isomer thereof.47. The method of claim 45 , wherein the alkane is unsubstituted.48. The method of claim 45 , wherein the alkane is linear.49. The method of claim 45 , wherein the alkane is converted to a diol by introduction of two hydroxy groups.50. The method of claim 44 , wherein a saturated fatty acid is hydroxylated.51. The method of claim 50 , wherein the saturated fatty acid is selected from the group consisting of butanoic acid claim 50 , pentanoic acid claim 50 , hexanoic acid claim 50 , heptanoic acid claim 50 , octanoic acid claim 50 , nonanoic acid ...

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

ENZYMATIC HYDROXYLATION OF ALIPHATIC HYDROCARBON

The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons. 117-. (canceled)18. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted , linear or branched , aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 , comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide having peroxygenase activity; wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 , SEQ ID NO:3 , SEQ ID NO:5 , SEQ ID NO:6 , SEQ ID NO:7 , or SEQ ID NO:8.19. The method of claim 18 , wherein the carbon in position 2 or 3 claim 18 , which is hydroxylated claim 18 , is unsubstituted until it is contacted with the peroxygenase.20. The method of claim 18 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:9.21. The method of claim 18 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:10.22. The method of claim 18 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:11.23. The method of claim 18 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:12.24. The method of claim 18 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:13.25. The method of claim 18 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:14.26. The method of claim 18 , wherein the polypeptide has at least 95% sequence identity to SEQ ID NO:1 claim 18 , SEQ ID NO:3 claim 18 , SEQ ID NO:5 claim 18 , SEQ ID NO:6 claim 18 , SEQ ID NO:7 claim 18 , or SEQ ID NO:8.27. The method of claim 18 , wherein the polypeptide comprises or consists of the amino acid sequence of SEQ ID NO:1 claim 18 , SEQ ID NO:3 claim 18 , SEQ ID NO:5 claim 18 , SEQ ID NO:6 claim 18 , SEQ ID NO:7 claim 18 , or SEQ ID NO:8.28. The method of claim 18 , wherein the polypeptide is a fragment of SEQ ID NO:1 ...

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Thielavia hyrcaniae.

Enzymatic hydroxylation of aliphatic hydrocarbon

The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

With over cyclooxygenase activity polypeptide

本发明涉及具有过氧合酶活性的分离的多肽和编码这些多肽的多核苷酸。本发明还涉及包括这些多核苷酸的核酸构建体、载体以及宿主细胞连同产生和使用这些多肽的方法。编码过氧合酶的多核苷酸分离自弗格斯毁丝霉。

Epoxidation using peroxygenase

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alkene, or a terpene.

Polypeptides Having Peroxygenase Activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 143.-. (canceled)44. A nucleic acid construct or expression vector comprising a polynucleotide encoding polypeptide having peroxygenase activity , wherein the polypeptide has at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2 and the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.45. The nucleic acid construct or expression vector of claim 44 , wherein the polypeptide has at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2.46. The nucleic acid construct or expression vector of claim 44 , wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2.47. The nucleic acid construct or expression vector of claim 44 , wherein the polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.48. The nucleic acid construct or expression vector of claim 44 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1 claim 44 , (ii) the cDNA sequence thereof claim 44 , or (iii) the full-length complement of (i) or (ii).49. The nucleic acid construct or expression vector of claim 44 , wherein the polypeptide is encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 claim 44 , or the cDNA sequence thereof.50. The nucleic acid construct or expression vector of claim 44 , wherein the polypeptide is a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution claim 44 , deletion claim 44 , and/or ...

Enzymatic Hydroxylation of Aliphatic Hydrocarbon

The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Myceliophtora hinnulea.

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Enzymatic hydroxylation of aliphatic hydrocarbon

The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

Polypeptides Having Peroxygenase Activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. 143-. (canceled)44. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted , linear or branched , alkane or saturated fatty acid having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 , comprising contacting the alkane or saturated fatty acid with hydrogen peroxide and a polypeptide having peroxygenase activity and at least 90% sequence identity to the sequence of the mature polypeptide of SEQ ID NO: 2 , wherein the alkane or saturated fatty acid is optionally substituted with one or two substituents selected from the group consisting of halogen , hydroxyl , carboxyl , amino , nitro , cyano , thiol , sulphonyl , formyl , acetyl , methoxy , ethoxy , phenyl , benzyl , xylyl , carbamoyl and sulfamoyl.45. The method of claim 44 , wherein an alkane is hydroxylated.46. The method of claim 45 , wherein the alkane is pentane claim 45 , hexane claim 45 , heptane claim 45 , octane claim 45 , nonane claim 45 , decane claim 45 , undecane claim 45 , dodecane claim 45 , tridecane claim 45 , tetradecane claim 45 , pentadecane or hexadecane claim 45 , or an isomer thereof.47. The method of claim 45 , wherein the alkane is unsubstituted.48. The method of claim 45 , wherein the alkane is linear.49. The method of claim 45 , wherein the alkane is converted to a diol by introduction of two hydroxy groups.50. The method of claim 44 , wherein a saturated fatty acid is hydroxylated.51. The method of claim 50 , wherein the saturated fatty acid is selected from the group consisting of butanoic acid claim 50 , pentanoic acid claim 50 , hexanoic acid claim 50 , heptanoic acid claim 50 , octanoic acid claim 50 , nonanoic acid ...

EPOXIDATION USING PEROXYGENASE

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alkene, or a terpene. 115-. (canceled)16. A method for producing an epoxide , comprising contacting a non-cyclic aliphatic alkene or a terpene with hydrogen peroxide and a peroxygenase (EC 1.11.2.1) , wherein the peroxygenase has at least 90% sequence identity to SEQ ID NO: 1.17. The method of claim 16 , wherein the peroxygenase has at least 95% sequence identity to SEQ ID NO: 1.18. The method of claim 16 , wherein the peroxygenase has at least 97% sequence identity to SEQ ID NO: 1.19. The method of claim 16 , wherein the peroxygenase has at least 98% sequence identity to SEQ ID NO: 1.20. The method of claim 16 , wherein the peroxygenase has at least 99% sequence identity to SEQ ID NO: 1.21. The method of claim 16 , wherein the peroxygenase has the amino acid sequence of SEQ ID NO: 1.22. The method of claim 16 , wherein the amino acid sequence comprises the motif E-H-D-[G claim 16 ,A]-S-[L claim 16 ,I]-S-R (SEQ ID NO: 21).23. The method of claim 16 , wherein the aliphatic alkene has one or more substituents selected from the group consisting of halogen claim 16 , hydroxyl claim 16 , carboxyl claim 16 , amino claim 16 , nitro claim 16 , cyano claim 16 , thiol claim 16 , sulphonyl claim 16 , formyl claim 16 , acetyl claim 16 , methoxy claim 16 , ethoxy claim 16 , carbamoyl and sulfamoyl.24. The method of claim 23 , wherein the substituent(s) are selected from the group consisting of chloro claim 23 , hydroxyl claim 23 , carboxyl and sulphonyl.25. The method of claim 16 , wherein the aliphatic alkene consists of at least three carbons claim 16 , and has a carbon-carbon double bond at one end.26. The method of claim 16 , wherein the aliphatic alkene is propene claim 16 , butene claim 16 , pentene claim 16 , hexene claim 16 , heptene claim 16 , octene claim 16 , nonene claim 16 , decene claim 16 , undecene claim 16 , dodecene claim 16 , tridecene claim 16 , tetradecene claim 16 , ...

Selective hydroxylation of aliphatic hydrocarbon in peroxidase area

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Penicillium chrysogenum.

Epoxidation using peroxygenase

The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alkene, or a terpene.

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

RECOMBINANT HEME THIOLATE OXYGENASES

The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides. The invention also relates to improved methods of producing such polypeptides in yeasts. 18-. (canceled)9. A modified unspecific peroxygenase (UPO) comprising an amino acid sequence having at least 75% sequence identity to the polypeptide of SEQ ID NO:12 and having increased peroxygenase activity as compared to unmodified wild-type UPO12 , which is the polypeptide of SEQ ID NO:12 , wherein the modification is a modification of at least one amino acid corresponding to any one of amino acids 145-261 of the polypeptide of SEQ ID NO:12 and wherein the peroxygenase activity is increased by 1.3-fold or more when measured in an ABTS assay or a 2 ,6-DMP assay.10. The modified UPO of claim 9 , wherein the modification is a modification of at least one amino acid corresponding to any one of amino acids D145 claim 9 , E249 claim 9 , D253 claim 9 , and/or C256 of the polypeptide of SEQ ID NO:12.11. The modified UPO of claim 9 , comprising at least a mutation corresponding to D145Y claim 9 , D253N claim 9 , D2531 claim 9 , C256S claim 9 , and/or introduction of a stop codon at a position corresponding to C256 or E249 of SEQ ID NO:12.12. The modified UPO of claims 9 , wherein the peroxygenase activity is 1.5-fold claims 9 , 2.0-fold claims 9 , or more increased when measured in an ABTS assay or a 2 claims 9 ,6-DMP assay.13. The modified UPO of claim 9 , comprising SEQ ID NO:31 claim 9 , SEQ ID NO:32 claim 9 , SEQ ID NO:33 claim 9 , SEQ ID NO:34 claim 9 , SEQ ID NO:35 claim 9 , or SEQ ID NO:36 claim 9 , or an amino acid sequence having at least 80% claim 9 , 85% claim 9 , 90% claim 9 , 95% claim 9 , 97% claim 9 , 98% claim 9 , or 99% identity to SEQ ID NO:31 claim 9 , SEQ ID NO:32 claim 9 , SEQ ID NO:33 claim 9 , SEQ ID NO:34 claim 9 , SEQ ID NO:35 claim 9 , or SEQ ID NO:36.14. A modified unspecific peroxygenase (UPO) having increased peroxygenase activity as compared ...

Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

ENZYMATIC REDUCTION OF HYDROPEROXIDES

The invention relates to enzymatic methods for reducing the amount of fatty acid hydroperoxides in a fatty acid containing fat/oil product. 1. A method for reducing the amount of fatty acid hydroperoxides in a fatty acid containing product , comprising contacting the fatty acid hydroperoxide with a peroxygenase.2. (canceled)3. A method for bleaching a carotenoid containing dye , stain , or composition , comprising contacting the carotenoid with a lipoxygenase , oxygen , and a peroxygenase.4. The method of claim 1 , wherein the peroxygenase comprises an amino acid sequence which has at least 70% identity to SEQ ID NO: 1 claim 1 , 2 claim 1 , 3 claim 1 , 4 claim 1 , 5 claim 1 , 6 claim 1 , 7 claim 1 , 8 claim 1 , 9 claim 1 , 10 claim 1 , 11 claim 1 , 12 claim 1 , 13 claim 1 , 14 claim 1 , 15 claim 1 , 16 claim 1 , 17 claim 1 , 18 claim 1 , 21 claim 1 , 22 claim 1 , 23 claim 1 , 24 claim 1 , 25 claim 1 , 26 claim 1 , 27 claim 1 , or 28.5. The method of claim 1 , wherein the amino acid sequence of the peroxygenase comprises the motif: E-H-D-[G claim 1 ,A]-S-[L claim 1 ,I]-S-R (SEQ ID NO: 20).6. The method of claim 1 , wherein the peroxygenase comprises or consists of an amino acid sequence having at least 80% identity to the amino acid sequence of SEQ ID NO: 1 claim 1 , 2 claim 1 , 3 claim 1 , 4 claim 1 , 5 claim 1 , 6 claim 1 , 7 claim 1 , 8 claim 1 , 9 claim 1 , 10 claim 1 , 11 claim 1 , 12 claim 1 , 13 claim 1 , 14 claim 1 , 15 claim 1 , 16 claim 1 , 17 claim 1 , 18 claim 1 , 21 claim 1 , 22 claim 1 , 23 claim 1 , 24 claim 1 , 25 claim 1 , 26 claim 1 , 27 claim 1 , or 28.7. The method of claim 1 , wherein the peroxygenase comprises or consists of an amino acid sequence having at least 80% identity to the amino acid sequence of SEQ ID NO: 1.8. The method of claim 1 , wherein the peroxygenase comprises or consists of the amino acid sequence of SEQ ID NO: 1 claim 1 , 2 claim 1 , 3 claim 1 , 4 claim 1 , 5 claim 1 , 6 claim 1 , 7 claim 1 , 8 claim 1 , 9 claim 1 , 10 claim ...

Polypeptides Having Peroxygenase Activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from 143-. (canceled)44. An isolated polypeptide having peroxygenase activity , selected from the group consisting of:(a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has peroxygenase activity.46. The polypeptide of claim 44 , comprising or consisting of SEQ ID NO: 2 claim 44 , or the mature polypeptide of SEQ ID NO: 2.47. The polypeptide of claim 46 , wherein the mature polypeptide is amino acids 1 to 244 of SEQ ID NO: 2.48. A composition comprising the polypeptide of .49. A detergent composition claim 44 , comprising a surfactant and a polypeptide of .50. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted claim 44 , linear or branched claim 44 , aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 claim 44 , comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide of .51. A method for hydroxylation in position 2 or 3 of the ...

Polypeptides Having Peroxygenase Activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from 143-. (canceled)44. An isolated polypeptide having peroxygenase activity , selected from the group consisting of:(a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has peroxygenase activity.45. The polypeptide of which comprises an amino acid sequence including the motif:E-H-D-[G,A]-S-[L,I]-S-R, orR-[G,A]-P-C-P-X-[M,L]-N-[S,T]-L.46. The polypeptide of claim 44 , comprising or consisting of SEQ ID NO: 2 claim 44 , or the mature polypeptide of SEQ ID NO: 2.47. The polypeptide of claim 46 , wherein the mature polypeptide is amino acids 1 to 237 of SEQ ID NO: 2.48. A composition comprising the polypeptide of .49. A detergent composition claim 44 , comprising a surfactant and a polypeptide of .50. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted claim 44 , linear or branched claim 44 , aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 claim 44 , comprising ...

Polypeptides Having Peroxygenase Activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Myceliophthora fergusii.

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from 143-. (canceled)44. An isolated polypeptide having peroxygenase activity , selected from the group consisting of:(a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has peroxygenase activity.45. The polypeptide of claim 44 , which comprises an amino acid sequence including the motif:E-H-D-[G,A]-S-[L,I]-S-R, orR-[G,A]-P-C-P-X-[M,L]-N-[S,T]-L.46. The polypeptide of claim 44 , comprising or consisting of SEQ ID NO: 2 claim 44 , or the mature polypeptide of SEQ ID NO: 2.47. The polypeptide of claim 46 , wherein the mature polypeptide is amino acids 1 to 244 of SEQ ID NO: 2.48. A composition comprising the polypeptide of .49. A detergent composition claim 44 , comprising a surfactant and a polypeptide of .50. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted claim 44 , linear or branched claim 44 , aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 claim 44 , ...

POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from 1. An isolated polypeptide having peroxygenase activity , selected from the group consisting of:(a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has peroxygenase activity.210.-. (canceled)11. An isolated polynucleotide encoding the polypeptide of .12. A nucleic acid construct or expression vector comprising the polynucleotide of operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.13. A recombinant host cell comprising the polynucleotide of operably linked to one or more control sequences that direct the production of the polypeptide.14. (canceled)15. A method of producing a polypeptide having peroxygenase activity claim 11 , comprising:{'claim-ref': {'@idref': 'CLM-00013', 'claim 13'}, '(a) cultivating the host cell of under conditions conducive for production of the polypeptide; and'}(b) ...

Polypeptides Having Peroxygenase Activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from 143-. (canceled)44. An isolated polypeptide having peroxygenase activity , selected from the group consisting of:(a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2;(b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);(c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, or the cDNA sequence thereof;(d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and(e) a fragment of the polypeptide of (a), (b), (c), or (d) that has peroxygenase activity.451. The polypeptide of claim , which comprises an amino acid sequence including the motif:E-H-D-[G,A]-S-[L,I]-S-R, orR-[G,A]-P-C-P-X-[M,L]-N-[S,T]-L.464. The polypeptide of claim , comprising or consisting of SEQ ID NO: 2 , or the mature polypeptide of SEQ ID NO: 2.47. The polypeptide of claim 46 , wherein the mature polypeptide is amino acids 1 to 241 of SEQ ID NO: 2.484. A composition comprising a polypeptide of claim .494. A detergent composition claim 46 , comprising a surfactant and a polypeptide of claim .50. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted claim 44 , linear or branched claim 44 , aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3 claim 44 , ...

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Penicillium chrysogenum.

Polypeptides having peroxygenase activity

本发明涉及具有过氧合酶活性的分离的多肽和编码这些多肽的多核苷酸。本发明还涉及包括这些多核苷酸的核酸构建体、载体以及宿主细胞连同产生和使用这些多肽的方法。编码过氧合酶的多核苷酸分离自条纹拟盘多毛孢。

Novel oxygenase from fungi Daldinia and method for preparing thereof

본 발명은 Daldinia 속 곰팡이에서 유래된 신규 산소 첨가 효소 및 이의 생산 방법에 관한 것으로, 보다 구체적으로 산소 첨가 효소 및 이를 암호화하는 폴리뉴클레오티드, 발현 벡터, 형질전환체, 및 이를 이용한 산소 첨가 효소의 생산 방법에 관한 것으로, 상기 Daldinia 속 곰팡이에서 유래된 신규 산소 첨가 효소는 조효소 또는 환원효소가 요구되는 고가의 전자전달시스템이 필요하지 않고 다양한 산화반응 및 넓은 기질 특이성을 나타냄으로써 다양한 기질에 대해 C-H 결합에 산소를 첨가하여 C-H 생성물을 저렴하게 생산할 수 있다는 이점이 있다. The present invention relates to a novel oxygenation enzyme derived from a fungus of the genus Daldinia and a production method thereof, and more particularly, to an oxygenation enzyme and a polynucleotide encoding the same, an expression vector, a transformant, and a production method of an oxygenation enzyme using the same In this regard, the novel oxygenating enzyme derived from the Daldinia genus fungus does not require an expensive electron transport system requiring a coenzyme or a reductase, and exhibits various oxidation reactions and broad substrate specificity to oxygen to CH bonds for various substrates. There is an advantage that CH product can be produced inexpensively by adding

Enzymatic reduction of hydroperoxides

The invention relates to enzymatic methods for reducing the amount of fatty acid hydroperoxides in a fatty acid containing fat/oil product.

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides having peroxygenase activity

本发明涉及具有过氧合酶活性的分离的多肽和编码这些多肽的多核苷酸。本发明还涉及包括这些多核苷酸的核酸构建体、载体以及宿主细胞连同产生和使用这些多肽的方法。编码过氧合酶的多核苷酸分离自Thielavia hyrcaniae。

Detergent composition comprising fatty acid processing enzymes

A liquid detergent composition, preferably a liquid manual dishwashing detergent composition, comprising one or more hydroperoxy fatty acid producing enzymes selected from the group consisting of: arachidonate lipoxygenases, alpha-dioxygenases, and mixtures thereof, preferably alpha-dioxygenases, a surfactant system and a liquid carrier ( i.e., water). Methods of washing comprising the liquid detergent composition are also provided.

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Daldinia caldariorum.

In vitro medicinal products from prodrugs and their use

Gegenstand der Erfindung ist ein Verfahren zur Herstellung eines Arzneimittels, wobei ein Prodrug mit Bioaktivierung durch Cytochrom-P450 Enzyme mit mindestens einer Peroxygenase versetzt und das erhaltene Produkt isoliert wird. Weiterhin betrifft die Erfindung hierzu ausgewählte Prodrugs, wie Thienopyridine und die erfindungsgemäß erhaltenen Produkte und deren Verwendung. The subject matter of the invention is a process for the production of a medicament, in which a prodrug with bioactivation by cytochrome P450 enzymes is mixed with at least one peroxygenase and the product obtained is isolated. Furthermore, the invention relates to selected prodrugs such as thienopyridines and the products obtained according to the invention and their use.

Recombinant heme thiolase oxygenase

本发明涉及具有过加氧酶活性的多肽和包含这种多肽的组合物。本发明还涉及在酵母中生产这种多肽的改进的方法。

PROCESS FOR THE IMPROVEMENT OF THE BIODEGRADABILITY OF POLYOLEFIN MATERIALS

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

High-efficiency expression method and application of nonspecific peroxygenase GmaUPO

本发明提供了一种非特异性过氧合酶GmaUPO的高效表达方法及应用。本发明对GmaUPO发酵过程的诱导条件进行了优化，在最优发酵条件时，7L发酵罐中毕赤酵母产GmaUPO酶活力最高为17.81U/L，相比摇瓶发酵酶活力提高124倍，实现了GmaUPO在毕赤酵母工程菌中的高效表达。其次，探究GmaUPO催化饱和直链脂肪酸羟基化反应研究，结果显示，GmaUPO对C10‑C16链长的饱和直链脂肪酸有催化效果，且优于同等反应条件下MroUPO的催化效果。本发明的研究为GmaUPO的工业化生产及其在羟基脂肪酸高效合成工业中的应用提供重要的理论指导。

Enzymatic preparation of indigo dyes and in situ dyeing process

The invention relates to use of a peroxygenase for preparing indigo dyes and an in situ method for dyeing a textile with indigo dyes.

Polypeptides having peroxygenase activity

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Penicillium chrysogenum.

Process for the cell-free production of non-specific peroxygenases and their use

Die vorliegende Erfindung betrifft ein Verfahren zur zellfreien Herstellung von unspezifischen Peroxygenasen aus Pilzen und/oder genetischen Varianten davon, unter Einsatz von eukaryontischen Zelllysaten, vorzugsweise aus Pilzen, insbesondere filamentösen Pilzen, und deren Verwendung als Biokatalysatoren in oxyfunktionalisierenden Reaktionen. The present invention relates to a method for the cell-free production of unspecific peroxygenases from fungi and/or genetic variants thereof using eukaryotic cell lysates, preferably from fungi, in particular filamentous fungi, and their use as biocatalysts in oxyfunctionalizing reactions.

Detergent composition

A detergent composition, preferably a manual dishwashing detergent composition, comprising one or more diol synthases capable of converting one or more unsaturated fatty acids into one or more oxylipins, and a surfactant system comprising one or more anionic surfactants and one or more co-surfactants selected from the group consisting of amphoteric surfactant, zwitterionic surfactant, and mixtures thereof. Method of using the detergent composition comprising a surfactant system and the diol synthases are also provided.

细菌非特异性过加氧酶(bupo)及其方法和用途

本发明涉及蛋白质工程和生物催化领域，更具体而言涉及具有过加氧酶活性的新型多肽以及与其相关的方法和用途。提供了一种生产黑色素或黑色素样色素的方法，所述方法包括使用具有产生色素的活性的多肽，其中所述多肽选自：(a)包含当与图1的序列号16的至少200个连续氨基酸残基比对时具有至少50％成对序列同一性的氨基酸序列并包含至少两个下述基序的多肽：i)RXFWXRWXXGHQ；ii)LXXLXXCXD；iii)PRXXYH；iv)RXR[ML]ALQH；v)CXXL；vi)HXXIAXH；vii)DLXHXG；和viii)VDGXHHPV；其中X是任何氨基酸；(b)包含当与图2的序列号12的至少150个氨基酸残基比对时具有至少30％成对序列同一性的氨基酸序列并包含基序HXXXC的多肽，其中X是任何氨基酸；和(c)具有过加氧酶活性的所述(a)或(b)的多肽的片段。

Bacterial unspecific peroxygenases (bupo's) and methods and uses thereof

The invention relates to the field of protein engineering and biocatalysis, more in particular to novel polypeptides having peroxygenase activity, and to methods and uses related thereto. Provided is a method for the production of melanin or a melanin-like pigment, comprising the use of a polypeptide having pigment producing activity, wherein said polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 50% pairwise sequence identity when aligned to at least 200 consecutive amino acid residues of Seq. no. 16 of Figure 1, and comprising at least two of the following motifs: i) RXFWXRWXXGHQ; ii) LXXLXXCXD; iii) PRXXYH; iv) RXR[ML]ALQH; v) CXXL; vi) HXXIAXH; vii) DLXHXG; and viii) VDGXHHPV; wherein X is any amino acid; (b) a polypeptide comprising an amino acid sequence having at least 30% pairwise sequence identity when aligned to at least 150 amino acid residues of Seq. no. 12 of Figure 2, and comprising the motif HXXXC, wherein X is any amino acid; and (c) a fragment of the polypeptide of (a) or (b) that has peroxygenase activity.

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Bacterial unspecific peroxygenases (BUPO's) and methods and uses thereof

Novel polypeptides having peroxygenase activity, and methods and uses related thereto. A method for the production of melanin or a melanin-like pigment, comprising the use of a polypeptide having pigment producing activity, wherein said polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 50% pairwise sequence identity when aligned to at least 200 consecutive amino acid residues of Seq. no. 16 and comprising at least two of the following motifs: i) RXFWXRWXXGHQ; ii) LXXLXXCXD; iii) PRXXYH; iv) RXR[ML]ALQH; v) CXXL; vi) HXXIAXH; vii) DLXHXG; and viii) VDGXHHPV; (b) a polypeptide comprising an amino acid sequence having at least 30% pairwise sequence identity when aligned to at least 150 amino acid residues of Seq. no. 12, and comprising the motif HXXXC; and c) a fragment of the polypeptide of (a) or (b) that has peroxygenase activity.

Mutantes de la peroxigenasa inespecífica con alta actividad monooxigenasa y sus usos

La presente invención se refiere a una peroxigenasa inespecífica del hongo Agrocybe aegeritaobtenida mediante evolución molecular dirigida para facilitar su expresión funcional en forma activa, soluble y estable. La peroxigenasa descrita en la presente invención presenta un incremento significativo en su expresión funcional, una actividad monooxigenasa mejorada y una actividad peroxidasa disminuida, respecto a las actividades monooxigenasa y peroxidasa que presenta la peroxigenasa inespecífica silvestre de A. aegerita. La peroxigenasa de la invención es útil en procesos químicos, incluyendo transformaciones industriales como son la oxifuncionalización selectiva de enlaces carbono-hidrógeno de diversos compuestos orgánicos.

洗剤組成物

１種以上の不飽和脂肪酸を１種以上のオキシリピンに変換することができる１種以上のジオール合成酵素と、１種以上のアニオン性界面活性剤、並びに両性界面活性剤、双極性界面活性剤、及びこれらの混合物からなる群から選択される１種以上の共界面活性剤を含む界面活性剤系と、を含む洗剤組成物、好ましくは食器を手作業で洗うための洗剤組成物。界面活性剤系及びジオール合成酵素を含む洗剤組成物の使用方法も提供される。

洗剤組成物

洗涤剂组合物

本发明公开了一种洗涤剂组合物，优选手动盘碟洗涤洗涤剂组合物，其包含能够将一种或多种不饱和脂肪酸转化为一种或多种氧脂质的一种或多种二醇合酶；和表面活性剂体系，所述表面活性剂体系包含一种或多种阴离子表面活性剂和一种或多种选自两性表面活性剂、两性离子表面活性剂、以及它们的混合物的辅助表面活性剂。还提供了使用包含表面活性剂体系和所述二醇合酶的洗涤剂组合物的方法。

Methods for producing halogenated compounds

The present disclosure features a method of modulating production of a small halogenated organic compound with a peroxidase enzyme, and related compositions and methods of use thereof.

融合酶的制备方法及融合酶

本发明提供了一种融合酶的制备方法及融合酶，本发明的融合酶的制备方法首先构建融合酶质粒，之后提取融合酶质粒，并将其转入到毕赤酵母中，进行发酵表达；再利用毕赤酵母制备粗酶液，并进行融合酶的分离纯化。在非特异性过氧化酶(UPO)的突变体JaWa、SoLo、WamPa的C端融合6×His‑SUMO标签，以及融合酶在毕赤酵母中的诱导表达及其后期的分离纯化，JaWa可去除酶的过氧化活性，WamPa本身在有机溶剂中具有良好的稳定性，而SUMO标签能可有效提高酶的表达量，使之高效的催化反应。此外，将JaWa、SoLo、WamPa融合6×His‑SUMO标签后能够利用组氨酸与金属离子Ni 2+ 的螯合作用进行分离纯化，分离纯化后的酶催化活性大大提高。

拟鬼伞来源的非特异性过氧合酶突变体及其制备和应用

本发明公开了一种拟鬼伞来源的非特异性过氧合酶突变体及其制备和应用，所述非特异性过氧合酶突变体的氨基酸序列如SEQ ID NO.2所示，该突变体可以作为催化剂用于化合物C＝C键环氧化反应，且具有立体催化专一选择性，反应条件温和，操作简便等优势，在化合物氧化反应的催化生产中具有很好的应用前景。

非特异性过氧合酶突变体及其编码基因和应用

本发明公开了一种非特异性过氧合酶突变体及其编码基因和应用，所述突变体的氨基酸序列如SEQ ID NO.3所示。在本发明中，通过对非特异性过氧合酶rDcaUPO进行定点突变后发现，突变体rDcaUPO‑A161C对双氧水的耐受性增强，在10mM双氧水中孵育14h，半衰期为野生型的6倍，在100mM的双氧水中孵育处理2h后，仍能维持较高的酶活。因此，本发明的非特异性过氧合酶突变体可在生物催化过程中应用，具有良好的稳定性能，可在应用过程中寿命延长和提高催化效率，更适用于工业领域上的应用。

細菌非特異的ペルオキシゲナーゼ（ｂｕｐｏ）並びにその方法及び使用

本発明は、タンパク質工学及び生体触媒作用の分野に関し、より詳細には、ペルオキシゲナーゼ活性を有する新規ポリペプチド、並びにそれに関連する方法及び使用に関する。メラニン又はメラニン様色素の生成方法であって、色素生成活性を有するポリペプチドの使用を含む方法が提供され、上記ポリペプチドは、（ａ）図１の配列番号１６の少なくとも２００個の連続するアミノ酸残基にアラインメントされた場合、少なくとも５０％のペアワイズ配列同一性を有し、以下のモチーフ：ｉ）ＲＸＦＷＸＲＷＸＸＧＨＱ；ｉｉ）ＬＸＸＬＸＸＣＸＤ；ｉｉｉ）ＰＲＸＸＹＨ；ｉｖ）ＲＸＲ［ＭＬ］ＡＬＱＨ；ｖ）ＣＸＸＬ；ｖｉ）ＨＸＸＩＡＸＨ；ｖｉｉ）ＤＬＸＨＸＧ；およびｖｉｉｉ）ＶＤＧＸＨＨＰＶ；（式中、Ｘは任意のアミノ酸である）の少なくとも２つを含むアミノ酸配列を含むポリペプチド；（ｂ）図２の配列番号１２の少なくとも１５０個のアミノ酸残基にアラインメントされた場合、少なくとも３０％のペアワイズ配列同一性を有するアミノ酸配列を含み、モチーフＨＸＸＸＣ（式中、Ｘは任意のアミノ酸である）を含む、ポリペプチド；並びに（ｃ）ペルオキシゲナーゼ活性を有する、（ａ）又は（ｂ）のポリペプチドの断片からなる群から選択される。

一种多突变位点的非特异性过加氧酶及其在合成25-羟基维生素d3中的应用

本发明公开了一种多突变位点的非特异性过加氧酶及其在合成25‑羟基维生素D3中的应用，以SEQ ID No.2所示氨基酸序列为出发序列，将第124位精氨酸突变为亮氨酸，第182位苯丙氨酸突变为丙氨酸，第265位的甲硫氨酸突变为缬氨酸，第310位的甘氨酸突变为脯氨酸；在pH＝7～8的磷酸钾缓冲液中，分别添加羟丙基‑β‑环糊精、维生素D3、过氧化氢以及非特异性过加氧酶组成反应混合液，在35～45℃下进行氧化反应24h，生成25‑羟基维生素D3，反应条件温和，无需使用有机溶剂，反应体系更绿色环保，转化率高，大大提高了25‑羟基维生素D3的制备效率，具有较好的工业应用前景。

一种非特异性过氧合酶的重组菌株及其构建方法和应用

本发明公开一种非特异性过氧合酶的重组菌株及其构建方法和应用，属于酶工程应用领域。所述重组菌株以pPICZαA质粒为出发载体，以毕赤酵母作为宿主菌，表达非特异性过氧合酶GmaUPO；所述非特异性过氧合酶GmaUPO的氨基酸序列如SEQ ID NO：1所示。本发明通过基因工程技术，成功在毕赤酵母中异源表达了GmaUPO，并优化了发酵条件，进行了重组酶的催化应用的初步研究，旨在扩大UPO生物酶的资源库，以替代现有的化学法进行芳香烃的高效清洁生产，同时为其催化应用提供一定的研究基础。

固定化载体及其制备方法、光酶催化剂及制备方法和应用、手性醇的合成方法

本发明提供了一种固定化载体及其制备方法、光酶催化剂及其制备方法和应用、手性醇的合成方法，该固定化载体能够固定非特异性过氧合酶，其包括基体，以及包覆在所述基体外的生物涂层；所述基体为具有生物相容性的半导体光催化剂，且所述半导体光催化剂具有开裂的介孔结构。采用本发明的固定化载体固定非特异性过氧合酶合成的光酶催化剂具有高效光催化性能且酶活性持久，在合成手性醇中，可以实现高效光酶耦联催化。

Verfahren zur peroxygenase-katalysierten abspaltung von benzyloxycarbonyl-schutzgruppen

Die vorliegende Erfindung betrifft ein Verfahren zur Abspaltung von Benzyloxycarbonyl-Schutzgruppen, dadurch gekennzeichnet, dass eine N-Benzyloxycarbonyl-Verbindung oder N-(4-Methoxybenzyloxycarbonyl)-Verbindung in einem wasserhaltigen Lösungsmittel in Gegenwart einer Peroxygenase aus Basidiomyceten mit Wasserstoffperoxid umgesetzt wird.

Method for hydroxylation of bile acids or analogues thereof

a method for hydroxylation of bile acids or analogues thereof Aspects of the disclosure provide method for 7B-hydroxylation of Formula I compound or analogue or salt thereof catalyzed by an enzyme in the presence of a peroxide. Additional aspects also relate to method for converting Formula I compound or analogue or salt thereof to Formula 11 compound or analogue or salt thereof in the presence of an enzyme and a peroxide; a corresponding reaction mixture; and the associated use of the enzyme to catalyze 7B-hydroxylation of Formula I compound or analogue or salt thereof

Methods for producing halogenated compounds

The present disclosure features a method of modulating production of a small halogenated organic compound with a peroxidase enzyme, and related compositions and methods of use thereof. Wherein production of the compound includes (I) providing a small organic compound (e.g., acetyl acetone); (ii) contacting the small organic compound with a peroxidase (e.g., a VHPO) to form a reaction mixture under conditions sufficient to produce a small halogenated organic compound; (ill) evaluating the small halogenated organic compound produced; and thereby modulating production of a small halogenated organic compound.

Methods for producing halogenated compounds

The present disclosure features a method of modulating production of a small halogenated organic compound with a peroxidase enzyme, and related compositions and methods of use thereof.

非特异性过氧合酶突变体及其应用

本发明公开了一种非特异性过氧合酶突变体及其应用，所述突变体的氨基酸序列为在非特异性过氧合酶rCviUPO的氨基酸构成发生R10K和R36K的位点突变、或发生R33K和R36K的位点突变。本发明的非特异性过氧合酶rCviUPO突变体rCviUPO‑R10K‑R36K和rCviUPO‑R33K‑R36K，对双氧水的耐受性增强，且相对于野生型，其酶活性更好，可在生物催化过程中应用，具有良好的稳定性能，可在应用过程中寿命延长和提高催化效率，更适用于工业领域上的应用。

Enzymes, micro-organisms and uses thereof, and a method of degrading a polyolefin

The present invention relates to the fields of life sciences, micro-organisms and degradation of polyolefin polymers. Specifically, the invention relates to an isolated specific enzyme or a fragment thereof, wherein said enzyme or fragment is capable of degrading a polyolefin, and to a micro-organism or a host cell comprising the enzyme or a fragment thereof. Also, the present invention relates to a polynucleotide encoding the enzyme or fragment thereof, and to an expression vector or plasmid comprising the polynucleotide of the present invention. And still, the present invention relates to use of the enzyme, fragment, micro-organism, host cell, polynucleotide, expression vector or plasmid of the present invention for degrading a polyolefin: to a method of degrading a polyolefin with the specific enzyme or a fragment thereof: and to a method of producing the enzyme or fragment thereof of the present invention.

小皮伞来源的非特异性过氧合酶突变体及其制备和应用

本发明公开了小皮伞来源的非特异性过氧合酶突变体及其制备和应用，所述非特异性过氧合酶突变体的氨基酸序列如SEQ ID NO.2所示，该突变体可以催化β‑紫罗兰酮在C‑3位发生羟基化生产3‑羟基β紫罗兰酮，且具有立体选择性强，底物转化率高，产物纯度高等优点，对3‑羟基β紫罗兰酮的生产和应用具有重要意义。

Verfahren zur zellfreien herstellung von unspezifischen peroxygenasen

Die vorliegende Erfindung betrifft ein Verfahren zur zellfreien Herstellung von unspezifischen Peroxygenasen vorzugsweise aus Pilzen und/oder genetisch veränderten Varianten davon unter Einsatz eukaryontischer Zellextrakte, vorzugsweise von Pilzen, insbesondere filamentöser Pilze, und dessen Verwendung.

In-vitro arzneimittel aus prodrugs und deren verwendung

Gegenstand der Erfindung ist ein Verfahren zur Herstellung eines Arzneimittels, wobei ein Prodrug mit Bioaktivierung durch Cytochrom-P450 Enzyme mit mindestens einer Peroxygenase versetzt, und das erhaltene Produkt isoliert wird. Weiterhin betrifft die Erfindung hierzu ausgewählte Prodrugs, wie Thienopyridine und die erfindungsgemäß erhaltenen Produkte und deren Verwendung.

Verfahren zur peroxygenase-katalysierten abspaltung von benzyloxycarbonyl-schutzgruppen

Mutantes de la peroxigenasa inespecífica con alta actividad monooxigenasa y sus usos

La presente invención se refiere a una peroxigenasa inespecífica del hongo Agrocybe aegeritaobtenida mediante evolución molecular dirigida para facilitar su expresión funcional en forma activa, soluble y estable. La peroxigenasa descrita en la presente invención presenta un incremento significativo en su expresión funcional, una actividad monooxigenasa mejorada y una actividad peroxidasa disminuida, respecto a las actividades monooxigenasa y peroxidasa que presenta la peroxigenasa inespecífica silvestre de A. aegerita. La peroxigenasa de la invención es útil en procesos químicos, incluyendo transformaciones industriales como son la oxifuncionalización selectiva de enlaces carbono-hidrógeno de diversos compuestos orgánicos.

一种非特异性过氧合酶的重组菌株及其构建方法和应用

本发明公开一种非特异性过氧合酶的重组菌株及其构建方法和应用，属于酶工程应用领域。所述重组菌株以pPICZαA质粒为出发载体，以毕赤酵母作为宿主菌，表达非特异性过氧合酶GmaUPO；所述非特异性过氧合酶GmaUPO的氨基酸序列如SEQ ID NO：1所示。本发明通过基因工程技术，成功在毕赤酵母中异源表达了GmaUPO，并优化了发酵条件，进行了重组酶的催化应用的初步研究，旨在扩大UPO生物酶的资源库，以替代现有的化学法进行芳香烃的高效清洁生产，同时为其催化应用提供一定的研究基础。

Method of heterologous expression of active fungal unspecific peroxygenase in bacterial host cells for fatty-acid epoxidation and other oxygenation reactions

The present invention relates to a method for heterologous expression of active fungal unspecific peroxygenase and/or variants thereof in bacterial host cells, preferably in Escherichia coli . The invention also relates to the recombinant active fungal unspecific peroxygenase and/or variants thereof thus obtained and to the use thereof. The invention further relates to a method for epoxidation of unsaturated fatty acids, or oxyfunctionalization of other organic compounds, comprising the recombinant active fungal unspecific peroxygenase and/or variants thereof obtainable by the method disclosed herein.

非特异性过氧合酶GmaUPO的高效表达方法及应用

本发明提供了一种非特异性过氧合酶GmaUPO的高效表达方法及应用。本发明对GmaUPO发酵过程的诱导条件进行了优化，在最优发酵条件时，7L发酵罐中毕赤酵母产GmaUPO酶活力最高为17.81U/L，相比摇瓶发酵酶活力提高124倍，实现了GmaUPO在毕赤酵母工程菌中的高效表达。其次，探究GmaUPO催化饱和直链脂肪酸羟基化反应研究，结果显示，GmaUPO对C10‑C16链长的饱和直链脂肪酸有催化效果，且优于同等反应条件下MroUPO的催化效果。本发明的研究为GmaUPO的工业化生产及其在羟基脂肪酸高效合成工业中的应用提供重要的理论指导。

In Vitro Drugs from Prodrugs and Use Thereof

An object of the invention is a process for the preparation of a pharmaceutical, wherein a prodrug with bioactivation by cytochrome P450 enzymes is mixed with at least one peroxygenase, and the product obtained is isolated. Furthermore, the invention relates thereto to selected prodrugs, such as thienopyridines and the products obtained according to the invention and their use.

Unspecific peroxygenase enzyme variants for selective fatty acid epoxidation or hydroxylation

The invention relates to a recombinant Marasmius rotula unspecific peroxygenase (rMroUPO) and two mutants thereof, wherein said mutants show enhanced selectivity towards either the epoxidation or the (sub)terminal ω/(ω-1)-hydroxylation of unsaturated fatty acids. The invention also refers to the use of these enzyme variants for the specific epoxidation or hydroxylation of fatty acids such as oleic acid, linoleic acid and/or alpha-linolenic acid.

Unspecific peroxygenase enzyme variants for selective fatty acid epoxidation or hydroxylation

The invention relates to a recombinant Marasmius rotula unspecific peroxygenase (rMroURO) and two mutants thereof, wherein said mutants show enhanced selectivity towards either the epoxidation or the (sub)terminal ω/(ω- 1)-hydroxylation of unsaturated fatty acids. The invention also refers to the use of these enzyme variants for the specific epoxidation or hydroxylation of fatty acids such as oleic acid, linoleic acid and/or alpha-linolenic acid.

非特异性过氧合酶突变体及其应用

本发明公开了一种非特异性过氧合酶突变体及其应用，所述突变体的氨基酸序列为在非特异性过氧合酶rCviUPO的氨基酸构成发生R10K和R36K的位点突变、或发生R33K和R36K的位点突变。本发明的非特异性过氧合酶rCviUPO突变体rCviUPO‑R10K‑R36K和rCviUPO‑R33K‑R36K，对双氧水的耐受性增强，且相对于野生型，其酶活性更好，可在生物催化过程中应用，具有良好的稳定性能，可在应用过程中寿命延长和提高催化效率，更适用于工业领域上的应用。

非特异性过氧合酶突变体及其应用

本发明公开了一种非特异性过氧合酶突变体及其应用，所述突变体的氨基酸序列如SEQ ID NO:3所示或如SEQ ID NO:5所示。本发明的非特异性过氧合酶MroUPO突变体F160C和I84C的最适过氧化氢浓度不变，在40mM过氧化氢的环境孵育90min，仍然保存33.8％和17.2％的残余酶活力，半衰期分别为野生型的6倍和5倍。因此，本发明的非特异性过氧合酶突变体可在生物催化过程中应用，具有良好的稳定性能，可在应用过程中寿命延长和提高催化效率，更适用于工业领域上的应用，初步解决了UPO在工业生产中的瓶颈问题，为其在产业化、商品化提供了工具指导。

一种非特异过氧化酶的组合表达系统及其应用

本发明涉及生物工程技术领域，尤其涉及一种非特异过氧化酶的组合表达系统及其应用。该非特异过氧化酶的组合表达系统，包括宿主细胞和转化入所述宿主细胞的表达载体，所述表达载体包括插入有编码非特异过氧化酶蛋白UPO的基因的第一表达载体和插入有编码二硫键异构酶PDI的基因的第二表达载体，所述宿主细胞为毕赤酵母(Komagataellaphaffii)GS115。本发明构建二硫键异构酶与非特异过氧化酶共表达的组合表达系统，使得非特异过氧化酶的表达量显著提高，由于二硫键异构酶在宿主胞内表达，而非特异过氧化酶能够分泌表达到宿主细胞外，所以收集的培养基上清中非特异过氧化酶浓度增加的同时不会产生其他蛋白，最终在5升生物反应器中非特异过氧化酶蛋白浓度可以达到333.3mg/L。