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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 48462. Отображено 100.
17-06-2019 дата публикации

Устройство для окрашивания крахмалосодержащего образца для микроскопического исследования

Номер: RU0000190069U1
Автор: Валентин Бабкенович Акопян, Вероника Алексеевна Сухарева, Давид Юрьевич Мартиросян, Левон Юрьевич Мартиросян, Максим Алексеевич Драгун, Юрий Цатурович Мартиросян
Принадлежит: Федеральное государственное бюджетное учреждение науки Институт биохимической физики им. Н.М. Эмануэля Российской академии наук (ИБХФ РАН)

Полезная модель относится к средствам для исследования крахмалосодержащих объектов и может быть использована для получения окрашенных образцов для микроскопического исследования распределения крахмальных зерен в тканях растительного и иного происхождения. Устройство содержит генератор воздушного потока, соединенный через регулятор потока с проточной закрытой емкостью, содержащей источник паров йода, соединенной, в свою очередь, через воздуховодную линию с устанавливаемой на столике микроскопа прозрачной проточной кюветой, содержащей исследуемый образец. Устройство удобно для выполнения серийных рутинных исследований, т.к. позволяет исключить ручные операции перемещения и предварительной обработки образцов в ходе их подготовки для анализа, исключает применение агрессивных реагентов при проведении йодокрахмальной реакции и позволяет проводить микроскопическое исследование непосредственно в кювете, в которой происходит проявление крахмальных зерен парами йода.

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Номер записи: 1
17-11-2020 дата публикации

Стенд для газодинамических испытаний

Номер: RU0000200896U1
Автор: Александр Викторович Елизаров, Александр Константинович Жохов, Алексей Павлович Костюнин, Анатолий Андреевич Брагинец, Андрей Юрьевич Бойко, Евгений Дмитриевич Орлов, Кирилл Евгеньевич Мазин, Сергей Анатольевич Дымнич
Принадлежит: Федеральное государственное бюджетное учреждение "33 Центральный научно-исследовательский испытательный институт" Министерства обороны Российской Федерации

Полезная модель относится к области анализа материалов путем определения их химических и физических свойств, а именно, к подготовке образцов для исследования путем их разбавления, распыления и смешивания. Предлагаемый стенд для газодинамических испытаний может быть использован при оценке аналитических эксплуатационных характеристик, а также показателей надежности технических средств химического контроля (ТС ХК) в условиях моделирования воздействия парогазовой смеси (ПГС) на испытуемые ТС ХК не только в нормальных климатических условиях, но и при различных климатических воздействиях (при температурах от минус 50°С до 60°С). Техническим результатом, обеспечивающим приведенную совокупность признаков, является возможность контроля создаваемой концентрации целевого компонента ПГС с заданной точностью в режиме реального времени, что в условиях отрицательных или положительных температур позволяет оперативно изменять параметры работы отдельно взятых блоков установки, направленных на создание ПГС в заданном диапазоне концентраций целевого компонента и проведении испытаний ТС ХК. Полезная модель стенда для газодинамических испытаний состоит из газодинамической установки, содержащей: диффузионный дозатор с переменной поверхностью испарения целевого компонента, смеситель газовых потоков для смешения газа-носителя и чистого воздуха, систему разбавления, включающую несколько последовательно расположенных ступеней, каждая из которых состоит из капилляра и фильтра-поглотителя, создавая парогазовую смесь с заданной концентрацией, которая поступает в установленный внутри камеры холода, тепла и влаги теплообменный радиатор, где достигается и поддерживается единая температура ПГС и испытуемого образца, а также осуществляется постоянный контроль концентрации целевого компонента в ПГС в режиме реального времени путем непрерывного попеременного отбора ПГС на две сорбционные трубки с последующей десорбцией пробы в кварцевую капиллярную колонку газового хроматографа и детектированием ...

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Номер записи: 2
26-01-2012 дата публикации

Apparatus for execution of treatment operations on microscope slides with tissue specimens

Номер: US20120017830A1
Автор: Oystein Ljungmann, Torstein Ljungmann
Принадлежит: Dako Instrumec AS

An apparatus for automatic execution of different treatment operations in connection with staining of tissue specimens on microscope slides, wherein the apparatus ( 1 ) comprises an assembly of vessels ( 4 ) for receiving different liquids for staining of the tissue specimens, a loading station ( 2 ) for microscope slides ( 28 ), a conveyor ( 5 ) for transfer of carriers with microscope slides from vessel to vessel in accordance with a treatment program, an unloading station ( 8 ) for treated microscope slides, and a control unit ( 18 ) for controlling the treatment operations in accordance with a data program. The apparatus comprises different levels (I, II) having units for execution of the relevant treatment operations. Thus, a first level (I) comprises the loading station ( 2 ) and the assembly of said vessels ( 4 ) with the appurtenant conveyor ( 5 ) and a second level (II) comprises a station ( 6 ) for application of cover glasses on the stained microscope slides ( 28 ), a succeeding station ( 7 ) for drying of the cover-slipped microscope slides, and the unloading station ( 8 ), a means ( 9 ) being provided for gripping and transfer of carriers ( 10 ) with stained microscope slides from the first level (I) to the cover-slipping station ( 6 ) on the second level (II).

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Номер записи: 3
26-01-2012 дата публикации

Methods and Devices for Rapid Urine Concentration

Номер: US20120021407A1
Автор: Yousef Haj-Ahmad
Принадлежит: Norgen Biotek Corp

The present invention provides a device for the concentration of one or more target analytes contained in a urine sample. The device comprises a tube comprising an upper portion defining an opening for receiving the urine sample and a lower tapered portion terminating in collection reservoir. The tube contains a predetermined amount of a particulate binding agent which specifically binds the one or more target analytes and of a predetermined amount of a binding buffer. The device comprises means for seating the opening of the tube. The present invention further provides methods and kits for concentrating one or more target analytes in murine sample.

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Номер записи: 4
23-02-2012 дата публикации

System for creation of formulations and generation of denaturation graphs

Номер: US20120045367A1
Автор: Burleigh Hutchins, Ernesto Freire, Richard Brown
Принадлежит: Avia Biosystems LLC

A system for automatically creating a denaturation curve is disclosed. In accordance with certain embodiments, a movement system including a unit having a plurality of cannulas is used. The cannulas are in fluid communication with a fluid system, which allows the cannulas to draw in and dispense fluid. A measurement system is included which draws fluid from a well into a detector to determine a characteristic of the fluid. A controller is used to control these systems and also to create a denaturation graph from the measured characteristics. In another embodiment, a plurality of formulations may be created using the system.

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Номер записи: 5
01-03-2012 дата публикации

Cell isolation apparatus

Номер: US20120052556A1
Автор: Akane Suzuki, Sunao Takeda, Takahiro SHIOYAMA
Принадлежит: Nihon Kohden Corp

A cell isolation apparatus includes: a cylinder member that is inserted into a container into which a reagent is to be introduced; a net which is stretched over the cylinder member; a tissue acquiring unit that is projected from the cylinder member; and a bottom lid portion that is configured by a bottomed pipe in which a first hole that communicates an interior with an exterior is formed, and that is fitted to a bottom portion of the cylinder member in a state where the net and the tissue acquiring unit are accommodated.

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Номер записи: 6
08-03-2012 дата публикации

In situ heat induced antigen recovery and staining method

Номер: US20120058570A1
Автор: Lee Angros
Принадлежит: Individual

An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. The reaction conditions for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide.

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Номер записи: 7
12-04-2012 дата публикации

Ion Beam Sample Preparation Apparatus and Methods

Номер: US20120085923A1
Автор: John Andrew Hunt, Steven Thomas Coyle
Принадлежит: Gatan Inc

Disclosed are embodiments of an ion beam sample preparation apparatus and methods for using the embodiments. The apparatus comprises an ion beam irradiating means in a vacuum chamber that may direct ions toward a sample, a shield blocking a portion of the ions directed toward the sample, and a shield retention stage with shield retention means that replaceably and removably holds the shield in a position. The shield has datum features which abut complementary datum features on the shield retention stage when the shield is held in the shield retention stage. The shield has features which enable the durable adhering of the sample to the shield for processing the sample with the ion beam. The complementary datum features on both shield and shield retention stage enable accurate and repeatable positioning of the sample in the apparatus for sample processing and reprocessing. Additionally, apparatus kits are disclosed that enable the use of the same shields in the observation of prepared samples.

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Номер записи: 8
03-05-2012 дата публикации

Encapsulated reagents and methods of use

Номер: US20120107834A1
Автор: Lee H. Angros
Принадлежит: Individual

The present invention contemplates use of encapsulated aqueous and non-aqueous reagents, solutions and solvents and their use in laboratory procedures. These encapsulated aqueous or non-aqueous reagents, solutions and solvents can be completely contained or encapsulated in microcapsules or nanocapsules that can be added to an aqueous or non-aqueous carrier solution or liquid required for medical and research laboratory testing of biological or non-biological specimens.

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Номер записи: 9
14-06-2012 дата публикации

Sample Collection System And Method

Номер: US20120144897A1
Автор: Robert S. Johnson, Stephen Scheufele
Принадлежит: Horizon Technology Inc

An apparatus or method for removing water and concentrating an analyte in solution, wherein the concentrated analyte sample is delivered directly to a vial, such as an autosampler vial that is capable of use in a gas chromatography autosampler.

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Номер записи: 10
28-06-2012 дата публикации

Sample measuring apparatus and sample measuring method

Номер: US20120164684A1
Автор: Hideaki Matsumoto, Takaaki Nagai, Yuichi Hamada
Принадлежит: Sysmex Corp

A sample measuring apparatus of measuring a component in a biological sample, comprising: an input section for inputting a sample species of a biological sample; a measurement sample preparation section for preparing a measurement sample by mixing the biological sample with a reagent; a first measurement section; a second measurement section being different from the first measurement section; a measurement sample supply section for supplying the measurement sample prepared in the measurement sample preparation section to at least one of the first measurement section and second measurement section; and a control section for controlling the measurement sample supply section based on the inputted sample species.

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Номер записи: 11
19-07-2012 дата публикации

Determination of fetal aneuploidy by quantification of genomic dna from mixed samples

Номер: US20120183963A1
Автор: Barb Ariel Cohen, Daniel Shoemaker, Mehmet Toner, Ravi Kapur, Roland Stoughton, Ronald W. Davis
Принадлежит: General Hospital Corp, GPB Scientific Inc, Verinata Health Inc

The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample.

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Номер записи: 12
02-08-2012 дата публикации

Slide Processing Apparatus and Method

Номер: US20120195497A1
Автор: Alan Prile, Ian Kerrod, John Temple, Roger Smith, Stephen Hankey
Принадлежит: Thermo Shandon Ltd

A basket processing apparatus that includes a basket storage portion in a form of a horizontal load rail. A plurality of slide storage baskets can be located in series on the rail. Each basket is ‘side loading’ and the side loading aperture of each basket can be selectively covered by a slide retaining bar. When the baskets are placed on the load rail, the basket can be moved along the horizontal rail by a basket moving means. The arrangement of the rail and the basket moving means is such that the baskets can be pushed together and move as a ‘train’. Baskets moved to the pick-up end of the rail can then be removed from the rail and processed. A vertical lift mechanism can be used to remove the basket and transport it to a processing device, for example a coverslipper.

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Номер записи: 13
27-09-2012 дата публикации

Sample measuring apparatus and sample measuring method

Номер: US20120244573A1
Автор: Hideaki Matsumoto, Takaaki Nagai, Yuichi Hamada
Принадлежит: Sysmex Corp

A method for analyzing blood cells in a whole blood sample obtained from a cat is provided. An electrical measurement result and an optical measurement result of the whole blood sample are acquired. The electrical measurement result is obtainable by electrically measuring blood cells in the whole blood sample and the optical measurement result is obtainable by optically measuring blood cells in the whole blood sample. On the basis of the electrical measurement result and the optical measurement result, volume of red blood cells in the whole blood sample is calculated.

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Номер записи: 14
04-10-2012 дата публикации

Dyes for Membranes and Biological Structures

Номер: US20120251458A1
Автор: Acacio Alves de Souza Lima Filho, Diogo de Sousa Martins, Mauricio MAIA, Rubens Belfort, JR.
Принадлежит: Kemin Industries Inc

The present invention relates to the use of one or more natural dyes, alone or in combination with other dyes for preparation of a composition to stain membranes and biological structures, in order to facilitate their identification during surgical procedures. The present invention relates to the use of one or more natural dyes, alone or in combination with other dyes, to stain membranes and biological structures, in order to facilitate their identification during surgical procedures.

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Номер записи: 15
18-10-2012 дата публикации

Method of detecting tumor cells by fluorescence signals

Номер: US20120264165A1
Автор: Karine Steenkeste, Marie-Pierre Fontaine Aupart, Monique Fabre, Pascal Eschwege, Sophie Ferlicot
Принадлежит: Assistance Publique Hopitaux de Paris APHP, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS, Universite Paris Sud Paris 11

The invention relates to a method of detecting dividing cells, or cells having the potential of dividing, in a cytological specimen stained using a Papanicolaou staining process, by the detection of a fluorescence signal coming from the membranes of these cells.

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Номер записи: 16
25-10-2012 дата публикации

Method and apparatus for detaching and/or isolating a histological sample

Номер: US20120266696A1
Автор: Annika Raatz, Arne Burisch, Christian LÖCHTE, Hermann Ulbrich, Karl-Heinrich WESTERHOFF
Принадлежит: LEICA BIOSYSTEMS NUSSLOCH GMBH

The invention provides a method for detaching and/or isolating a histological sample that is adhering to another sample and/or to the inside of a cassette, for example as a result of solidification of an embedding medium within a cassette. The sample is immobilized in a sample receiving chamber above a first fill level of a liquid that is suitable for counteracting the adhesion; and that the fill level of the liquid is then elevated at least until said level reaches the sample. An apparatus according to the present invention that can be used for carrying out the method comprises a sample receiving chamber and an adjusting chamber, the adjusting chamber being connected to the sample receiving chamber in such a way that a change in the fill level height of the liquid in the sample receiving chamber is producible by changing the pressure existing in the adjusting chamber.

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Номер записи: 17
15-11-2012 дата публикации

Automatic dilution for multiple angle light scattering (mals) instrument

Номер: US20120287435A1
Автор: John A. Adams, Steven Stephenson
Принадлежит: JMAR LLC

A method for detecting and identifying a particle in a liquid, the system comprises controlling the provisioning of a water sample using a computer controlled metering pump; mixing the water sample with particle free filtered water to provide a diluted water sample when required; at the end of a measurement interval, determining a Total Counts Per Minute (TCPM) for the diluted water sample; determining an additional counts per minute from the sample (SCPM) for the diluted water sample; if the SCPM is greater then a Lower Optimum count Rate (LOCR) and less than a Upper Optimum Count Rate (UOCR), then setting a dilution ratio (DR); and correcting an events classification based on the DR.

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Номер записи: 18
06-12-2012 дата публикации

Enhanced spot preparation for liquid extractive sampling and analysis

Номер: US20120304747A1
Автор: Gary J. Van Berkel, Richard C. King
Принадлежит: UT Battelle LLC

A method for performing surface sampling of an analyte, includes the step of placing the analyte on a stage with a material in molar excess to the analyte, such that analyte-analyte interactions are prevented and the analyte can be solubilized for further analysis. The material can be a matrix material that is mixed with the analyte. The material can be provided on a sample support. The analyte can then be contacted with a solvent to extract the analyte for further processing, such as by electrospray mass spectrometry.

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Номер записи: 19
06-12-2012 дата публикации

On-line sampling from a process source

Номер: US20120305464A1
Автор: Sylvain Cormier
Принадлежит: Waters Technologies Corp

An online sample manager of a liquid chromatography system includes a fluidic tee having a first inlet port, a second inlet port, and an outlet port. A diluent pump moves diluent from a diluent source to the first inlet port of the fluidic tee. A valve has a fluidic intake port connected to a process source for acquiring a process sample therefrom. A pumping system moves the acquired process sample from the valve into the second inlet port of the fluidic tee where the process sample merges with the diluent arriving at the first inlet port to produce a diluted process sample that flows out from the outlet port of the fluidic tee.

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Номер записи: 20
13-12-2012 дата публикации

Imaging system and techniques

Номер: US20120312957A1
Автор: Bikash Sabata, Chris Todd, Glenn Stark, Gregory L. LONEY
Принадлежит: BioImagene Inc, Ventana Medical Systems Inc

Systems and techniques for an optical scanning microscope and/or other appropriate imaging system includes components for scanning and collecting focused images of a tissue sample and/or other object disposed on a slide. The focusing system described herein provides for determining best focus for each snapshot as a snapshot is captured, which may be referred to as “on-the-fly focusing.” The devices and techniques provided herein lead to significant reductions in the time required for forming a digital image of an area in a pathology slide and provide for the creation of high quality digital images of a specimen at high throughput.

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Номер записи: 21
13-12-2012 дата публикации

Image Classifier Training

Номер: US20120314930A1
Автор: Clifford C. Hoyt, Richard Levenson
Принадлежит: Individual

Methods are disclosed that include: (a) applying a first stain to a first sample having a plurality of regions, where the first stain selectively binds to only a first subset of the regions of the first sample; (b) applying a second stain to the first sample, where the second stain binds to a second set of regions of the first sample; (c) obtaining an image of the first sample, and analyzing the image to obtain a first component image corresponding substantially only to spectral contributions from the first stain, and a second component image corresponding substantially only to spectral contributions from the second stain; and (d) training a classifier to identify regions of a second sample based on information derived from the first and second component images, the identified regions corresponding to the first subset of regions of the first sample.

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Номер записи: 22
20-12-2012 дата публикации

Instrument and Method for Clinical Examinations and Cleaning Method Therefor

Номер: US20120318302A1
Автор: Yoshiyuki Nakayama
Принадлежит: Jeol Ltd

An automated clinical analyzer and method is offered which can clean the nozzles of a reaction cuvette wash unit. A first detergent is put in first reagent containers located on a first reagent turntable. A computer controller drives a first reagent pipette to aspirate the detergent from the first reagent containers and to deliver the detergent into reaction cuvettes. The controller drives a reaction turntable to bring each reaction cuvette holding the detergent therein to the reaction cuvette wash unit. The controller drives the reaction cuvette wash unit to aspirate the detergent from inside the reaction cuvettes using reaction cuvette wash nozzles to thereby clean the wash nozzles. A second detergent is then used to clean the nozzles.

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Номер записи: 23
20-12-2012 дата публикации

System and method for preparing samples

Номер: US20120322052A1
Автор: Kurt J. Halverson, Matthew T. Scholz, Stephen C.P. Joseph
Принадлежит: 3M Innovative Properties Co

A system and method for preparing samples for analyte testing. The sample preparation system can include a freestanding receptacle. The method can include providing a liquid composition comprising a source and a diluent, and positioning the liquid composition in a reservoir defined by the freestanding receptacle. The method can further include filtering the liquid composition to form a filtrate comprising an analyte of interest, removing at least a portion of the filtrate from the sample preparation system to form a sample, and analyzing the sample for the analyte of interest.

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Номер записи: 24
27-12-2012 дата публикации

Method of Pre-Treatment and Staining of a Biological Sample and Device for Support of a Biological Sample and Methods of Using Such Device

Номер: US20120330564A1
Автор: David A. Stanforth, Lars Winther, Loren L. Bland, Marc E. Key, Martin N. Lindberg
Принадлежит: DakoCytomation Denmark AS

There is disclosed a method of pre-treatment and staining, according to a protocol, of a biological sample disposed upon the surface of a carrier, the method comprising the step of recording at least one parameter relating to at least one protocol step in a non-volatile memory located either upon or within the carrier or a device incorporating the carrier. Also disclosed is a device comprising: a non-volatile memory; a surface of the device adapted to carry a biological sample; and communications means electrically coupled to the memory for enabling data transmission to or from an external apparatus. Also disclosed is a method of controlling processing of a biological sample disposed upon a carrier, comprising: providing, upon or within the carrier or an apparatus holding the carrier, a non-volatile memory having information relating to sample processing priority or protocol; reading the information; and scheduling the processing based upon the information.

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Номер записи: 25
24-01-2013 дата публикации

Fluid sample preparation systems and methods

Номер: US20130019697A1
Автор: Brian J. Mckeen, Eric D. YEATON, James L. Dowling
Принадлежит: Constitution Medical Inc

Sample application systems can include an extraction mechanism to remove a sample from sample containers, a sample vessel disposed on a deployment mechanism, where the deployment mechanism is arranged to move the sample vessel to receive a sample, an extraction mechanism washing station to wash the extraction mechanism, a sample applicator to remove a portion of the sample in the sample vessel and apply it onto a sample carrier, where the deployment mechanism can move the sample vessel to a sample application position, a sample vessel washing station to wash the sample vessel, where the deployment mechanism can move the sample vessel to the sample vessel washing station, a sample applicator washing station to wash the sample applicator after the sample has been dispensed onto the sample carrier, and a fluid control system to control flow of a fluid provided to the extraction mechanism and the sample applicator.

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Номер записи: 26
24-01-2013 дата публикации

Sample applicator sensing and positioning

Номер: US20130021461A1
Автор: David J. Zahniser, Eric LEKNES, Frank L. Pawlowski, Michael Zahniser, Russell ZAHNISER, Stephen Conroy
Принадлежит: Constitution Medical Inc

Systems and methods for positioning a sample applicator relative to a substrate include: (a) obtaining an image of the sample applicator in proximity to the substrate, where the image includes a direct image region corresponding to the sample applicator and a first reflected image region corresponding to an image of the sample applicator reflected from a surface of the substrate; (b) determining a position of an edge of the sample applicator in the direct image region; (c) determining a position of a reflected edge of the sample applicator in the first reflected image region; (d) determining a distance between the edge of the sample applicator and the reflected edge of the sample applicator; and (e) determining the position of the sample applicator relative to the substrate based on the distance between the edges.

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Номер записи: 27
24-01-2013 дата публикации

Method for attaching rfid tag of memory cassette for tissue specimen and memory cassette for tissue specimen having rfid tag

Номер: US20130022518A1
Автор: Byung Gyu Park, Kwang Il Choi, Yong Pill Kim
Принадлежит: TIME SYSTEM CO Ltd

Disclosed are a method for attaching an RFID tag to a memory cassette for tissue specimens and a memory cassette for tissue specimens having the RFID tag attached thereto, in which the RFID tag is attached to the memory cassette for tissue specimens and the RFID tag operates stably even when the memory cassette having the RFID tag attached thereto comes in contact with chemicals or is submerged in various chemical solutions. The method includes forming the memory cassette for tissue specimens provided on an inclined plane of a front surface of a body with an RFID tag insertion groove, inserting the RFID tag into the RFID tag insertion groove provided on the inclined plane of the body, and attaching a protective cap onto the top of the inclined plane of the body in which the RFID tag is inserted into the RFID tag insertion groove, and coupling the protective cap to the inclined plane of the body by ultrasonic welding to adhere the RFID tag to the body of the memory cassette for tissue specimens. Accordingly, the RFID tag can operate stably even when the memory cassette for tissue specimens having the RFID tag attached thereto comes in contact with chemicals or is submerged in chemical solutions or water.

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Номер записи: 28
31-01-2013 дата публикации

Method for avoiding influence of endogenous lipoprotein and reagent

Номер: US20130029429A1
Автор: Takayuki Abe, Tetsuya Ota, Yuki Takahashi
Принадлежит: Sekisui Medical Co Ltd

To identify the aforementioned interference component present in serum or plasma, to thereby provide means for avoiding any interference effect caused by the component.

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Номер записи: 29
21-02-2013 дата публикации

Clearing reagent for biological material, and use thereof

Номер: US20130045503A1
Автор: Asako Sakaue, Atsushi Miyawaki, Hiroshi Hama, Hiroshi Kurokawa, Hiroyuki Kawano
Принадлежит: RIKEN Institute of Physical and Chemical Research

A clearing reagent according to this invention for making a biological material transparent is a solution containing, as an active component, at least one compound selected from the group consisting of urea and urea derivatives, in order to provide a novel clearing reagent for making a biological material transparent.

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Номер записи: 30
14-03-2013 дата публикации

Liquid handling plunger for a biological sample in a tube

Номер: US20130064736A1
Автор: David Daf
Принадлежит: Taigen Bioscience Corp

A plunger for mixing a biological sample with a reagent in a tube is moved up and down reciprocally to agitate the biological sample and the reagent for subsequent extraction of the material from the mixture to be analyzed. The plunger's agitation facilitates the concentration of the extracted material to reach an extent where a correct analysis is possible. The plunger of the present invention has a shape of a hollow cylinder with an aperture at the bottom, a plurality of slots formed on the cylindrical surface, and an opening formed at the top. The plunger allows a pipette to draw the extracted material in the test tube out from the opening at the top of the plunger without removing the plunger, and prevents drawing out the fragments of the sample after the biological sample and the reagent have been sufficiently mixed.

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Номер записи: 31
21-03-2013 дата публикации

Sensor and Method for Removing Interfering Substance

Номер: US20130068000A1
Автор: Iketani Kazuya, Katsuki Koji, Shiraki Yasunori
Принадлежит: ARKRAY, INC.

A technique is provided, wherein any influence, which would be otherwise exerted on a reaction of an objective substance caused by a reagent enzyme by an interfering substance contained in a specimen, is suppressed in relation to an electrochemical sensor for measuring the objective substance contained in the specimen. A sensor comprises a substrate; a detecting unit which is provided on the substrate and which detects an objective substance; a filter which covers the detecting unit, which permits permeation of the objective substance on one hand, and which regulates permeation of an interfering substance contained in a sample on the other hand; and removing unit which removes the interfering substance adhered to the filter. 1. A sensor for measuring an objective substance contained in a sample , the sensor comprising:a substrate;a detecting unit which is provided on the substrate and which detects the objective substance;a filter which covers the detecting unit, which permits permeation of the objective substance on one hand, and which regulates permeation of an interfering substance contained in the sample on the other hand; anda removing unit which removes the interfering substance adhered to the filter.2. The sensor according to claim 1 , wherein the removing unit removes the interfering substance adhered to the filter by vibrating the filter.3. The sensor according to claim 2 , wherein the removing unit includes:a piezoelectric element which is vibrated by applying a voltage; anda vibration transmitting unit which is fixed to the piezoelectric element and which transmits vibrational energy of the piezoelectric element to the filter.4. The sensor according to claim 1 , wherein the removing unit removes the interfering substance adhered to the filter by supplying claim 1 , to the filter claim 1 , an agent for decomposing the interfering substance.5. The sensor according to claim 4 , wherein the removing unit includes:a piezoelectric element which is vibrated by ...

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Номер записи: 32
21-03-2013 дата публикации

Rapid analyte collection and testing device

Номер: US20130068040A1
Автор: Glen Ford, Lawrence Loomis, Leslie Kirkegaard, Robert Greenfield
Принадлежит: Individual

A rapid analyte collection and testing device, said device comprising a) a casing, said casing having) a first casing section, said first casing section containing an encapsulated buffer section containing a buffer, and asecond casing section, said second casing section comprising a window on a side of said second section, a complementary mechanism for attachment to said first casing section, an opening at a proximal end of said second casing section; and a non permeable platform strip positioned lengthwise within and extending beyond the second casing section. The non-permeable platform further contains a swab, said swab positioned at the distal end of said non-permeable platform strip, a lateral flow assay positioned downstream from said swab, and a tag, positioned upstream from the capture reagent site.

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Номер записи: 33
21-03-2013 дата публикации

Field and storage chemical test kit

Номер: US20130071301A1
Автор: De Weerd Jan W., Forsythe John M.
Принадлежит:

Methods and kits for assaying a chemical in a sample are disclosed. The method includes placing an extraction solution and an internal standard in a container. A sample is collected from a first location and placed in the container. The container is transported to a second location where the chemical in the extraction solution is assayed. A kit for transporting the sample from a first location to a second location includes at least one container for holding the sample. The at least one container includes an extraction solution for dissolving a chemical in the solution and an internal standard for calibrating an assay of the chemical. The methods and kits may be used in a system for quantitating an amount of a sprout inhibitor on a tuber, such as a potato. 1. A kit for transporting a tuber sample from a crop storage facility to a chemical analysis facility comprising at least one container configured to hold and transport the tuber sample from the crop storage facility to the chemical analysis facility , the container comprising:an extraction solution for dissolving a sprout inhibitor on the tuber sample at the crop storage facility, wherein the extraction solution comprises a solvent selected from the group consisting of ethyl acetate, tributyl phosphate, cyclohexane, dichloromethane(methyl chloride), trimethylpentane, dibutyl ether, acetonitrile, toluene, heptane, a substituted aromatic solvent, a halogenated alkyl alcohol, an ether, ethanol, propanol, isopropanol, tetrahydrfurfuryl alcohol, and any combination thereof;an internal standard for calibrating a quantitative measurement of the sprout inhibitor, which quantitative measurement is made at the chemical analysis facility; andan indicator identifying a known amount of internal standard.2. The kit of claim 1 , wherein the internal standard comprises 2-ethylnaphthalene claim 1 , wherein the extraction solution comprises ethanol and trimethylpentane claim 1 , and wherein the sprout inhibitor is a substituted ...

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Номер записи: 34
21-03-2013 дата публикации

Sample preparation disposable devices and sample collection and preparation methods using same

Номер: US20130071944A1
Автор: April L. Fiorelli, M. Scott Ulrich, R. Reade Harpham
Принадлежит: Battelle Memorial Institute Inc

An article of manufacture embodiment comprises a sample cartridge including a sample substrate, and an enclosure including a sample ingress port. The enclosure mates with the sample cartridge to define a sample container containing the sample substrate which is accessible in the sample container via the sample ingress port. The sample cartridge including the sample substrate is removable from the sample container. A sampling method embodiment comprises disposing a sample on a sample substrate in a sample container and removing a sample cartridge including the sample substrate from the sample container. An article of manufacture embodiment comprises a sample substrate, a sample container containing the sample substrate and including a sample ingress port providing access to the sample substrate in the sample container, and a sample cartridge including the sample substrate. The sample cartridge including the sample substrate is removable as a unit from the sample container.

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Номер записи: 35
21-03-2013 дата публикации

Method of Pooling and/or Concentrating Biological Specimens for Analysis

Номер: US20130072387A1
Автор: Robert M. Lloyd, JR., Ronald O. Gilcher
Принадлежит: Vivebio LLC

The present invention provides methods for concentrating and pooling liquid suspensions of biological specimens containing analytes of interest in a dry state. The dried biological specimens containing analytes of interest are reconstituted and released from the matrix for subsequent analysis in concentrated form.

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Номер записи: 36
28-03-2013 дата публикации

MICROFLUIDIC DEVICE AND METHOD FOR ISOLATING TARGET USING SAME

Номер: US20130074613A1
Автор: Jeon Byung Hee
Принадлежит: CYTOGEN CO., LTD.

A micro-fluidic device includes a filter case, a first capture array and a second capture array. The filter case includes an inlet for introducing a sample containing different kinds of targets, an outlet for discharging the sample and a channel extending between the inlet and the outlet. The first capture array is arranged in an upstream portion of the channel, the first capture array including a plurality of first forward funnels arranged along a direction orthogonal to a flow direction of the sample so as to capture the different kinds of targets. The second capture array is arranged in a downstream portion of the channel, the second capture array including a plurality of second forward funnels arranged along the direction orthogonal to the flow direction of the sample so as to capture the different kinds of targets. 1. A micro-fluidic device , comprising:a filter case including an inlet for introducing a sample containing different kinds of targets, an outlet for discharging the sample and a channel extending between the inlet and the outlet;a first capture array arranged in an upstream portion of the channel, the first capture array including a plurality of first forward funnels arranged along a direction orthogonal to a flow direction of the sample so as to capture the different kinds of targets; anda second capture array arranged in a downstream portion of the channel, the second capture array including a plurality of second forward funnels arranged along the direction orthogonal to the flow direction of the sample so as to capture the different kinds of targets.2. The micro-fluidic device of claim 1 , further comprising: a reverse flow guide means for causing the different kinds of targets captured in the first and second forward funnels to flow toward the inlet in the direction opposite to the flow direction of the sample when the filter case is mounted in an overturned state.3. The micro-fluidic device of claim 2 , wherein the reverse flow guide means ...

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Номер записи: 37
28-03-2013 дата публикации

SAMPLE PREPARATION DEVICE

Номер: US20130078619A1
Автор: BELGRADER Phillip, COONEY Christopher G.
Принадлежит: AKONNI BIOSYSTEMS, INC.

A sample preparation device is disclosed. The sample preparation device includes a housing defining a passage way between a first opening and a second opening; and a sample filter occupying a section of said passage way. The sample filter contains a monolith adsorbent that specifically binds to nucleic acids. Also disclosed are sample filters containing glass frit is coated with an capture agent that binds specifically to an analyte of interest, sample filters containing a hydrophilic matrix with impregnated chemicals that lyses cell membranes, a cartridge base and an integrated sample preparation cartridge. 127-. (canceled)28. A method for purifying an analyte from a liquid sample , comprising:placing said liquid sample in a container; a housing defining a passage way between a first opening and a second opening; and', 'a filter occupying a section of said passage way, said sample filter comprising a material that specifically binds to said analyte,', 'wherein said portion of liquid sample is drawn into said housing via the first opening and passing through said filter, and wherein said analyte binds to said filter while passing through said filter;, 'withdrawing at least a portion of said liquid sample into a sample preparation device comprisingexpelling said portion of liquid sample from said sample preparation device via the first opening, wherein said portion of liquid sample passes through said filter a second time while exiting said sample preparation device; andeluting said analyte from said filter by withdrawing an eluting buffer into said sample preparation device via the first opening and expelling said eluting buffer from said sample preparation device via the first opening, wherein said eluting buffer passes through said filter while entering and exiting said sample preparation device.29. The method of claim 28 , further comprising:washing said filter by withdrawing a washing buffer into said sample preparation device via the first opening and expelling ...

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Номер записи: 38
28-03-2013 дата публикации

Method And Devices For The Cross-Referencing Of Identification Of Tissue Slice Supports For Microtomised Analytical Samples

Номер: US20130078669A1
Автор: Hans L. Heid, Jose Novoa
Принадлежит: MICROM INTERNATIONAL GMBH

The invention relates to a method and device for the cross-referencing of identification ( 1 ) of tissue slice supports ( 2 ), for microtomised analytical samples still to be mounted thereon, with identification information ( 3 ) of a tissue sample holder ( 4 ) of a tissue sample ( 5 ) which is not yet microtomised. The conventional problem of cross-referencing is improved in a simple manner, whereby the identification information ( 3 ) for the tissue sample holder ( 4 ) is automatically detected when positioned in the microtome ( 6 ) and an identification ( 1 ) corresponding thereto is automatically transferred to at least one tissue slice support ( 2 ) and that tissue slice support ( 2 ), provided with the identification ( 1 ), is dispensed for application of the tissue sample slice at the moment when a tissue sample slice must be applied to a tissue slice support ( 2 ).

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Номер записи: 39
28-03-2013 дата публикации

METHOD FOR PREPARING A SAMPLE FOR CHROMATOGRAPHIC SEPARATION PROCESSES AND SYSTEMS FOR CARRYING OUT A SAMPLE PREPARATION

Номер: US20130078735A1
Автор: DAVID Frank, Sandra Koen, Sandra Patrick, Sandra Tom, Tienpont Bart
Принадлежит: GERSTEL SYSTEMTECHNIK GMBH & KG

The invention relates to a method for preparing a sample for chromatographic separation processes, in which a sample vessel () is partially filled with a substance to be examined and is closed, the substance to be examined is subjected to a thermo-chemical reaction in which at least one sample component is converted into another substance, and in which by means of a removing device samples are removed from the sample vessel () for analytical examination, characterised in that the sample vessel () forms a cavity, into which the substance to be examined is introduced as a core and a heating section for indirect heat transfer is applied along the filling of substance to be examined. 1. A method for preparing a sample for chromatographic separation methods , in which a sample vessel is partially filled with a substance to be examined and is closed , the substance to be examined is subjected to a thermochemical reaction in which at least one sample component is converted into another substance , and in which samples are taken from the sample vessel by means of an extraction device for analytical examination , wherein the sample vessel forms a cavity into which the substance to be examined is introduced as a core and a heating section for indirect heat transfer is applied along the filling of substance to be examined.2. A method for preparing a sample for chromatographic separation methods , in which a sample vessel is partially filled with a substance to be examined and is closed , the substance to be examined is subjected to a thermochemical reaction in which at least one sample component is converted into another substance , and in which samples are taken from the sample vessel by means of an extraction device for analytical examination , wherein a hollow vessel is used as the sample vessel , which has a shaft that is at least partially filled with the substance to be examined and is exposed to a heat treatment , which is applied to the entire circumference of the ...

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Номер записи: 40
28-03-2013 дата публикации

Microarray-based sample analysis system

Номер: US20130079253A1
Автор: Aleksandr N. Perov, Christopher G. Cooney, Phillip Belgrader
Принадлежит: Akonni Biosystems Inc

A microarray-based sample analysis (MBSA) system includes a cartridge holder adapted to receive a replaceable cartridge that is configured to receive a detachable, replaceable sample analysis unit containing one or more reaction chambers for sample analysis; a fluid control subsystem that controls fluid flow; and an optical subsystem configured to capture an image of the microarray.

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Номер записи: 41
04-04-2013 дата публикации

Systems and Methods of Sample Processing and Temperature Control

Номер: US20130084567A1
Автор: Barber Michael, Buchanan Kristopher, Clark Robert, Favuzzi John, Guggenheimer Benno, Key Marc, Lathrop Bob, Welcher Rosanne
Принадлежит: DAKO DENMARK A/S

Systems and methods of sample processing and temperature control are disclosed. The invention may especially relate to temperature control, and may in some embodiments be methods of temperature control of an automated sample processing system and methods of automated sample processing. Specifically, the present invention provides temperature control in relation to sample processing systems and methods of processing samples, and in some embodiments provides temperature control in relation to sample carriers and processing materials such as reagents. Corresponding systems and devices are disclosed, including sample processing systems (), sample carrier temperature regulation systems (), reagent temperature regulation systems, sample processing control systems, and temperature regulation devices, among other embodiments. Scientific fields to which the present invention may have particular applicability include immunohistochemistry, hybridization, fluorescent in-situ hybridization, special staining, such as special staining of histological samples, microarray sample processing, and cytology, as well as potentially other chemical and biological applications. 195-. (canceled)96. A method for the automated processing of at least one sample on at least one carrier according to a processing protocol , comprising:positioning at least one reagent container within a reagent section;positioning at least one carrier retention device in at least one carrier section;regulating the temperature of the at least one sample via an active temperature regulation element to a set point and within a tolerance specified by the protocol;dispensing fluid on the at least one carrier via a moveable robotic member according to the processing protocol;monitoring the at least one carrier retention device;controlling the moveable robotic member according to the processing protocol; andexchanging at least one of the at least one reagent container and the at least one carrier retention device during ...

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Номер записи: 42
18-04-2013 дата публикации

AUTOMATED DEVELOPER FOR IMMUNO-STAINED BIOLOGICAL SAMPLES

Номер: US20130095500A1
Автор: Emery Michael P., Gonzales Raymond J., GROT Brian, Magavi Sanjay Shivayogi, Sinclair James E., Wallace Matthew C.
Принадлежит: Vertex Pharmaceuticals, Incorporated

Disclosed herein are systems and methods for the developing of immuno-stained biological samples. The systems disclosed herein are automated and are configured to control one or more steps of the developing procedure. Reagents may be added using automatic syringe dispensing. Reagent temperature, reagent stirring, and wash procedures are programmable and can be separately controlled for separate immuno-staining procedures that are performed at the same time. 1. An automated method of developing immuno-stained biological samples , comprising:providing at least a primary antibody in a first syringe;placing an undeveloped sample into an incubation box;contacting the undeveloped sample with a primary antibody by automatically activating a first motorized pusher to mechanically force the primary antibody from within the syringe barrel into the incubation box;automatically removing the primary antibody from within the incubation box after the contacting step;automatically pumping a wash buffer into the incubation box; andautomatically removing the wash buffer from the incubation box.2. The method of claim 1 , additionally comprising contacting the sample with a secondary antibody by activating a second motorized pusher to mechanically force a secondary antibody from within a second syringe into the incubation box.3. The method of claim 1 , wherein the primary and secondary antibodies are manually aspirated into the first and second syringes claim 1 , respectively.4. The method of claim 1 , said method further comprising automatically agitating the incubation following providing the primary antibody.5. The method of claim 1 , further comprising automatically stirring the primary and secondary antibodies within the first and second syringes.6. The method of claim 1 , further comprising automatically cooling the primary and secondary antibodies within the first and second syringes.7. An automated developer for immuno-stained biological samples claim 1 , comprising:a processor ...

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Номер записи: 43
02-05-2013 дата публикации

Holder for measuring permeability of unconsolidated sediment

Номер: US20130104629A1
Автор: Jun-Ho Oh, Kue-Young KIM, Tae-Hee Kim
Принадлежит: Korea Institute of Geoscience and Mineral Resources KIGAM

Disclosed is a holder for measuring a permeability of an unconsolidated sediment. The holder includes a sediment installation part having a solid sediment attached to opposite ends of an unconsolidated sediment along a longitudinal direction; a transverse pressure supply part installed to surround the sediment installation part to supply a predetermined transverse pressure to the sediment installation part by using a fluid supplied from the outside; and a longitudinal pressure supply part installed at opposite ends of the transverse pressure supply part to cover opposite ends of the sediment installation part to be moved along a vertical direction so as to supply a predetermined longitudinal pressure to the sediment installation part.

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Номер записи: 44
02-05-2013 дата публикации

HIGH THROUGHPUT AND VOLUMETRIC ERROR RESILIENT DILUTION WITH DIGITAL MICROFLUIDIC BASED LAB-ON-A-CHIP

Номер: US20130105318A1
Автор: Bhattacharya Bhargab B., CHAKRABARTY KRISHNENDU, Ghoshal Sarmishtha, ROY Sudip
Принадлежит: INDIAN STATISTICAL INSTITUTE

Systems and methods are provided for producing fluids with desired concentration factors from the given supply of any two concentration factors, one greater than the target CF and one less than the target CF, of the same fluid. According to one embodiment, a method is provided that stores intermediate waste droplets from a sequence of mix and split steps and repeats certain steps of the sequence using the stored intermediate waste droplets. Such a method may produce additional target CF droplets faster than repeating the entire sequence. In another embodiment, a method of volumetric error resilient target CF droplet generation has been described, and includes reusing the stored intermediate waste droplets and involves a collection of capacitive sensing circuits associated with some electrode platforms. 1. A method for producing a number (M) of diluted fluid droplets having a target concentration factor (CF) on a digital microfluidic (DMF) biochip , the biochip comprising a plurality of DMF-based electrode platforms arranged to carry out a sequence of mixing and splitting steps , the method comprising:determining a target CF for a end resultant fluid mixture;expressing the target CF as an N-bit binary fraction; mixing together two input sample fluid droplets having different CFs to produce a first resultant mixture having a given resultant CF;', 'splitting the first resultant mixture into a first resultant droplet and a second resultant droplet;', wherein when the N-bit binary fraction is a first binary value indicating that the resultant mixture produced in the given mixing step has a resultant CF larger than the target CF, mixing the first resultant droplet with a droplet of a first one of two input sample fluids in the next mixing step of the sequence of mixing steps;', 'wherein when the N-bit binary fraction is a second binary value indicating that the resultant mixture produced in the given mixing step has a resultant CF smaller than the target CF, mixing the ...

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Номер записи: 45
02-05-2013 дата публикации

MICROFLUIDIC SYSTEMS AND METHODS FOR REDUCING THE EXCHANGE OF MOLECULES BETWEEN DROPLETS

Номер: US20130109575A1
Автор: Brouzes Eric, Caron François, El Harrak Abdeslam, Griffiths Andrew David, Kleinschmidt Felix, Link Darren
Принадлежит: RAINDANCE TECHNOLOGIES, INC.

The present invention generally relates to systems and methods to create stable emulsions with low rates of exchange of molecules between microdroplets. 1. A method comprising the steps of:(a) providing within a carrier fluid a plurality of microdroplets comprising a first microdroplet comprising a first biological or chemical material and a second microdroplet comprising a second biological or chemical material, wherein the carrier fluid is immiscible with the first microdroplet and second microdroplet and comprises a first oil and a first surfactant at a first concentration within the first oil;(b) changing the carrier fluid, in the presence of the plurality of microdroplets, by changing (i) some or all of the first oil for a second oil, (ii) some or all of the first surfactant for a second surfactant, (iii) the first concentration to a second concentration, or any combination of (i), (ii) and/or (iii).2. The method of claim 1 , further comprising the step (c) of providing a microfluidic device and wherein step (a) further comprises providing the plurality of microdroplets and the carrier fluid in the microfluidic device and/or step (b) further comprises changing the carrier fluid within the microfluidic device.3. The method of claim 1 , wherein the first biological or chemical material and/or the second biological or chemical material comprises a tissue claim 1 , cell claim 1 , particle claim 1 , protein claim 1 , antibody claim 1 , amino acid claim 1 , nucleotide claim 1 , small molecule claim 1 , pharmaceutical claim 1 , and/or label.4. The method of claim 1 , wherein the first concentration is sufficient to stabilize the microdroplets against coalescing with each other in the first carrier fluid.5. The method of claim 4 , wherein the first concentration is determined claim 4 , at least in part claim 4 , based on stabilizing the microdroplets over a time frame determined by a reaction and/or detection of the one or more biological and/or chemical materials.6. ...

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Номер записи: 46
02-05-2013 дата публикации

Device analysis

Номер: US20130110421A1
Автор: Kay Lederer
Принадлежит: Plastic Logic Ltd

Performing an analysis of an electronic device sample by measuring a property at a plurality of points of said electronic device sample, and in advance of said analysis subjecting said plurality of points to at least one treatment that increases the difference in said property between at least two elements of said electronic device sample.

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Номер записи: 47
09-05-2013 дата публикации

Nucleic Acid Collection Device and Nucleic Acid Collection Amount Estimation Method

Номер: US20130112002A1
Автор: Kozuka Masahiro, Sukawa Yukihiro
Принадлежит: ARKRAY, INC.

The present invention provides a nucleic acid collection device that can estimate the nucleic acid collection amount when collecting nucleic acids from a biological sample containing nucleic acids. The nucleic acid collection device comprises a sucking and discharging unit for sucking in and forcing out a sample containing nucleic acids, a collector for collecting the nucleic acids by sucking in and forcing out the sample using the sucking and discharging unit, a pressure measurer for measuring a discharging pressure when forcing out the sample and a sucking pressure when sucking in the sample, and measuring a differential pressure that is the difference between the discharging pressure and the sucking pressure, and an estimator for estimating the collection amount of nucleic acids collected based on the differential pressure. 1. A nucleic acid collection device comprising:a sucking and discharging unit for sucking in and forcing out a sample containing nucleic acids;a collector for collecting the nucleic acids by sucking in and forcing out the sample using the sucking and discharging unit;a pressure measurer for measuring a discharging pressure when forcing out the sample and a sucking pressure when sucking in the sample, and measuring a differential pressure that is the difference between the discharging pressure and the sucking pressure; andan estimator for estimating the collection amount of nucleic acids collected based on the measured differential pressure.2. The nucleic acid collection device of claim 1 , wherein the estimator stores in advance correlation information between the differential pressure and the nucleic acid collection amount claim 1 , and estimates the collection amount of nucleic acids collected based on the differential pressure and the correlation information.3. The nucleic acid collection device of claim 1 , wherein the sucking and discharging unit forces out the sample and then sucks in the sample at high speed and further sucks in such at ...

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Номер записи: 48
09-05-2013 дата публикации

Microfluidic apparatus and microfluidic system having the same

Номер: US20130112296A1
Автор: Chung Ung Kim, Ki Ju Lee, Young Goun Lee
Принадлежит: SAMSUNG ELECTRONICS CO LTD

A microfluidic system capable of determining at least one of the existence and the amount of a fluid by use of an optical sensor even if the fluid is transparent. The microfluidic system includes a platform including at least one chamber configured to accommodate a fluid, at least one channel connected to the at least one chamber, and at least one protrusion disposed on an inner surface of the at least one chamber and configured to show a difference in transmittance according to the existence of the fluid.

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Номер записи: 49
09-05-2013 дата публикации

NEW LIQUID PROCESSING DEVICE

Номер: US20130112622A1
Автор: Hiesinger Margit, Müller Markus
Принадлежит: QIAGEN GmbH

The present invention refers to a device, comprising a hollow body having at least one open end and at least one barrier inside of the hollow body, which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force like pressure, drag force or driving power to said barrier, wherein said barrier is spaced from at least one open end of the hollow body, the use of such a device for processing of a liquid sample, a method for preparation of said device and a method for isolation or purification of any biomolecules using said device. 118.-. (canceled)19. A device , comprising (i) at least one hollow body , each hollow body having at least one open end; (ii) at least one barrier placed inside the hollow body spaced apart from the at least one open end of the hollow body to separate the hollow body into at least two compartments , wherein the barrier is non-permeable for liquids and solids under ambience conditions , but becomes liquid-permeable by applying an external force to said barrier; (iii) optionally at least one porous liquid-permeable element inside the hollow body; and (iv) optionally at least one removable closing device to seal at least one open end.20. The device of claim 19 , comprising (i) at least one hollow body claim 19 , each hollow body having an inlet and an outlet; (ii) at least one barrier placed above the outlet or adjacent of at least one of the liquid permeable element(s) if present; (iii) optionally at least one liquid-permeable element placed above the outlet; (iv) optionally at least one removable closing device to seal the inlet and/or outlet of the hollow body; and (v) optionally at least one collection tube to collect a mobile phase (eluate) after having passed the outlet.21. The device of claim 20 , wherein the liquid-permeable element is a porous frit claim 20 , filter claim 20 , fleece claim 20 , or membrane.22. The device according to claim 19 , comprising more than ...

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Номер записи: 50
09-05-2013 дата публикации

TISSUE ARRAY PRODUCTION METHOD

Номер: US20130115652A1
Автор: Arakawa Masahiko, Nakazawa Yoshiaki, Naritake Kohji, Takano Masaki
Принадлежит:

A tissue array production method which enables even roll-shaped tissue pieces having various diameters to be steadily fixed to a substrate block is provided. 1. A tissue array production method , wherein roll-shaped tissue pieces formed by rolling sheet-like tissue pieces in the shape of a roll are arranged on a substrate in an array form , the method comprising the steps of:placing a holding member which holds a plurality of roll-shaped tissue pieces so that their axis directions are vertically directed in a container into which a melted embedding medium is poured for its accumulation, to hold the respective roll-shaped tissue pieces in the holding member;pouring the embedding medium into the container, to form a substrate block constituted so that the plurality of roll-shaped tissue pieces is arranged in the array form; andslicing the substrate block so that the roll-shaped tissue pieces are ring-shaped, to place the sliced pieces on the substrate.2. The tissue array production method according to claim 1 , wherein the embedding medium is a paraffin.3. The tissue array production method according to claim 1 , wherein an elastic member including a plurality of insertion holes having at least the same diameters as the minimum diameter of the roll-shaped tissue piece is used as said holding member claim 1 , to hold the outer periphery of the roll-shaped tissue piece.4. The tissue array production method according to claim 3 , wherein said insertion hole is polygonal.5. The tissue array production method according to claim 1 , wherein a grid-like member including a plurality of grid squares with at least the same side length as the minimum diameter of the roll-shaped tissue piece is used as said holding member claim 1 , to hold the outer periphery of the roll-shaped tissue piece.6. The tissue at production method according to claim 1 , wherein bar-like holding members to be inserted into the axial cores of the plurality or roll-shaped tissue pieces are used as said ...

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Номер записи: 51
09-05-2013 дата публикации

Dilution method for digital microfluidic biochips

Номер: US20130115703A1
Автор: Bhargab B. Bhattacharya, Krishnendu Chakrabarty, Sudip Roy
Принадлежит: Indian Statistical Inst

Systems and methods are provided for producing fluids with desired concentration factors. According to one embodiment, a sequence of mix steps comprises mixing a resultant solution of a preceding mix step with one of the input solutions of the preceding mix step depending on a concentration factor of the resultant solution. If the concentration factor of the resultant solution is higher than the target concentration factor, then the resultant solution is mixed with the input solution having the lower concentration factor. If the concentration factor of the resultant solution is lower than the target concentration factor, then the resultant solution is mixed with the input solution having the higher concentration factor.

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Номер записи: 52
16-05-2013 дата публикации

CELL SEPARATION USING MICROCHANNEL HAVING PATTERNED POSTS

Номер: US20130121895A1
Автор: BHATT Ram S., Tang Zhongliang, TSINBERG Pavel
Принадлежит: BIOCEPT, INC.

A micro flow device () for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (). The posts are spaced across the width of an expanded collection chamber region ( ) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally. 120-. (canceled)21. A microflow apparatus comprising:a body having a flow path configured for cell capture, the flow path comprising an inlet passageway, an outlet, and a microchannel arrangement extending between said inlet passageway and outlet to form a collection region, the flow path sufficient to support a fluid flow rate in the range of 0.2 to 1 mm/second, andwhen in operation said microchannel is connected to a pump sufficient to support the continuous flow of liquid sample at a flow rate in the range of 0.2 to 1 mm/second,wherein:said microchannel arrangement includes a plurality of transverse separator posts being integral with a base surface and projecting therefrom, wherein said posts are arranged in a random and irregular pattern that prevents straightline flow and disrupts streamline flow of a liquid sample through the collection region, andthe inlet passageway is aligned at an angle of between about 30° to about 60° to said base surface.22. The apparatus of claim 21 , wherein said posts have variable cross-sectional sizes in the range of about 70 μM to ...

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Номер записи: 53
16-05-2013 дата публикации

POST PROTEIN HYDROLYSIS REMOVAL OF A POTENT RIBONUCLEASE INHIBITOR AND THE ENZYMATIC CAPTURE OF DNA

Номер: US20130122509A1
Автор: EWERT MATT
Принадлежит: UNIVERSITY OF SOUTH FLORIDA

The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample. 1. A method for extracting a nucleic acid from a biological sample , the method comprising:(a) contacting the biological sample with a endonuclease or exonuclease, whereby the nucleic acid in the biological sample is processed or cleaved;(b) contacting the biological sample with a single stranded binding protein (SSB) immobilized on a matrix, whereby the SSB binds to the processed or cleaved nucleic acid to produce an SSB-bound nucleic acid; and(c) extracting the SSB-bound nucleic acid from the biological sample.2. The method of claim 1 , wherein the SSB is biotinylated claim 1 , wherein the matrix has avidin or streptavidin attached to the surface claim 1 , and wherein the SSB is immobilized on the matrix via binding of the biotin to the avidin or streptavidin.3. The method of claim 1 , wherein the matrix is a magnetic bead.4. The method of claim 2 , wherein the matrix is a magnetic bead.5. The method of claim 1 , wherein the method further comprises circulating the biological sample at about 37 degrees Celsius.6. The method of claim 2 , wherein the method further comprises circulating the biological sample at about 37 degrees Celsius.7. The method of claim 1 , wherein the method further comprises heating the biological sample containing the SSB immobilized on a matrix to 90 to 100 degrees Celsius.8. The method of claim 2 , wherein the method further comprises heating the biological sample containing the SSB immobilized on a matrix to 90 to 100 degrees Celsius.9. The method of claim 1 , wherein the biological sample is blood.10. The method of claim 2 , wherein ...

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Номер записи: 54
16-05-2013 дата публикации

Microfluidic Assay

Номер: US20130122534A1
Автор: Lim Chwee Teck, Lim Eric, Tay Andee
Принадлежит:

A process for carrying out hematoxylin and eosin staining in a microfluidic device is described. 1. A process for carrying out hematoxylin and eosin staining of circulating tumour cells trapped in a microfluidic device , the device comprising a sample inlet and at least one outlet connected in fluid communication by a microfluidic channel , the microfluidic channel having a plurality of cell isolation wells positioned therein , each well comprising an array of isolation structures arranged to trap a circulating tumour cell , but allow passage of other blood constituents , wherein the process comprises:(a) flowing a blood sample through the channel and allowing trapping of circulating tumour cells by the isolation structure to occur;(b) washing by flowing a liquid through the channel;(c) flowing a fixative solution through the channel, wherein the fixative solution comprises paraformaldehyde and methanol;(d) washing by flowing a liquid through the channel to remove excess fixative; and(e) staining with hematoxylin solution;(f) washing by flowing a liquid through the channel and(g) staining with eosin solution.2. The process of claim 1 , comprising step (a′) claim 1 , prior to step (a) claim 1 , of priming the microfluidic device by flowing a priming solution through the channel.3. The process of claim 2 , wherein the priming solution is an EDTA solution.4. The process of claim 3 , wherein the priming solution is an 8-12 mM EDTA solution.5. The process of claim 4 , wherein the priming solution is an 8-12 mM EDTA solution in PBS.6. The process of claim 3 , wherein the EDTA priming solution has an EDTA concentration of about 10 mM.7. The process of claim 1 , wherein the fixative solution is a solution of paraformaldehyde and methanol in a solvent.8. The process of claim 7 , wherein the fixative solution is a solution in phosphate buffered saline (PBS).9. The process of claim 7 , wherein the fixative solution is a 3-5 w/v % paraformaldehyde solution claim 7 , containing ...

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Номер записи: 55
16-05-2013 дата публикации

METHOD FOR FORMING TISSUE PIECES FOR TISSUE ARRAY AND DEVICE FOR FORMING TISSUE PIECES FOR TISSUE ARRAY

Номер: US20130122540A1
Автор: Arakawa Masahiko, Nakazawa Yoshiaki, Naritake Kohji, Takano Masaki
Принадлежит:

The invention provides a method for forming tissue pieces which allows stable and accurate winding around a core rod in rolling a sheet-like tissue piece, and a device therefor. 1. A method for forming the tissue pieces for a tissue array , wherein the tissue pieces are used as each tissue piece in the tissue array obtained by arranging the tissue pieces on a substrate in an array form , and a sheet-like tissue piece is rolled to form a roll-shaped tissue piece , the method comprising the steps of:producing the sheet-like tissue piece by slicing a tissue block obtained by embedding tissues with an embedding medium;placing the sheet-like tissue piece on a mounting block;applying peeling means which easily enables peeling of the tissue piece from the mounting block, between the mounting block and the tissue piece; andwinding the tissue piece on the mounting block around the core rod while heating the tissue piece.2. The method for forming the tissue pieces for the tissue array according to claim 1 , wherein an embedding medium having a melting point lower than that of the embedding medium contained by the sheet-like tissue piece is coated on the mounting block in said peeling means.3. The method for forming the tissue pieces for the tissue array according to claim 1 , wherein said embedding medium is paraffin or the like.4. The method for forming the tissue pieces for the tissue array according to claim 1 , wherein an upper face of the mounting block is roughened in said peeling means.5. The method for forming the tissue pieces for the tissue array according to claim 1 , wherein said mounting block having a heating table incorporating a heater and a plate detachable from an upper face of the heating table is used claim 1 , and said peeling means is applied on an upper face of the plate.6. The method for forming the tissue pieces for the tissue array according to claim 1 , whereina sheet-like member having a melting point higher than that of the embedding medium ...

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Номер записи: 56
16-05-2013 дата публикации

METHODS FOR DETECTING CATECHOLAMINES BY MASS SPECTROMETRY

Номер: US20130122602A1
Автор: Caulfield Michael P., Lee Gloria Kwangja
Принадлежит: Quest Diagnostics Investments Incorporated

Provided are methods for determining the amount of one or more of one or more of epinephrine (E), norepinephrine (NE), and dopamine (D) in a sample using mass spectrometry. The methods generally involve ionizing one or more of E, NE, and D in a sample and detecting and quantifying the amount of the ion to determine the amount of one or more of E, NE, and D in the sample 1. A method for determining the amount of one or more analytes selected from the group consisting of epinephrine , norepinephrine , and dopamine , the method comprising:a. performing a protein precipitation for a urine sample provided at a pH below about 3 to generate a supernatant; i. if one of the one or more analytes is epinephrine, said ionization comprises generating ions with a mass to charge ratio of 166.1±0.50;', 'ii. if one of the one or more analytes is norepinephrine, said ionization comprises generating ions with a mass to charge ratio of 151.9±0.50; and', 'iii. if one of said one or more analytes is dopamine, said ionization comprises generating ions with a mass to charge ratio of 136.9±0.50; and, 'b. subjecting the supernatant to electrospray ionization (ESI) under conditions suitable to produce one or more ions detectable by mass spectrometry; whereinc. determining the amount of the one or more ions by mass spectrometry, wherebythe amount of the one or more ions reflects the amount of said one or more analytes in the sample.2. The method of claim 1 , wherein the pH is at about 2.5.3. The method of claim 1 , wherein the urine sample is provided in a solution comprising sodium metabisulfite.4. The method of claim 1 , wherein the analytes are purified by high performance liquid chromatography (HPLC) prior to ionization.5. The method of claim 1 , wherein said mass spectrometry is tandem mass spectrometry.6. The method of claim 1 , wherein one of the one or more analytes is epinephrine.7. The method of claim 6 , wherein said ionization further comprises generating ions with a mass to charge ...

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Номер записи: 57
16-05-2013 дата публикации

Etching Agent for Type II InAs/GaInSb Superlattice Epitaxial Materials

Номер: US20130122715A1
Автор: Edward H Aifer, Sergey I. Maximenko
Принадлежит: US Department of Navy

This disclosure involves a formula, mixing procedure, etching technique and application of an etchant for revealing defects in T2SL's grown lattice matched to (100) GaSb. The etching agent comprises a (2.5:4.5:16.5:280) solution by volume or (1%:2%:9%:88%) by weight, of HF:H 2 O 2 :H 2 SO 4 :H 2 O. The etchant is made by mixing (49%) hydrofluoric aqueous solution with (30%) water-based peroxide, followed by sulfuric acid, and diluted with de-ionized H2O (DI-water).

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Номер записи: 58
23-05-2013 дата публикации

Methods to Fix and Detect Nucleic Acids

Номер: US20130130260A1
Автор: Cekan Pavol, Ludwig Janos, Pena John, Rouhanifard Sara H., Sohn Cherin, Tuschl Thomas
Принадлежит: THE ROCKEFELLER UNIVERSITY

In one aspect, the invention relates to a method for fixing a short nucleic acid in a biological sample. In another aspect, the invention relates to a method for detecting a target short nucleic acid in a biological sample. The method includes contacting the biological sample with an aldehyde-containing fixative, and subsequently contacting the sample with a water-soluble carbodiimide. In a further aspect, the invention relates to a kit for fixing a short nucleic acid in a biological sample. The kit includes a support substrate for holding the sample; an aldehyde-containing fixative; and a water-soluble carbodiimide. 1. A method for fixing a short nucleic acid in a biological sample , said method comprisinga) contacting the biological sample with an aldehyde-containing fixative; andb) subsequently contacting the sample with a water-soluble carbodiimide.2. The method of claim 1 , wherein the aldehyde-containing fixative is formaldehyde.3. The method of claim 1 , wherein the carbodiimide is selected from the group consisting of: 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC); 1-Cyclohexyl-3-(2-morpholinyl-(4)-ethyl)carbodiimide metho-p-toluenesulfonate (CMC) claim 1 , N claim 1 ,N′-dicyclohexylcarbodiimide (DCC) claim 1 , and N claim 1 ,N′-diisopropylcarbodiimide (DIC).4. The method of claim 3 , wherein the carbodiimide is 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.5. The method of claim 4 , wherein the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide has a concentration of about 50 mM to about 250 mM.6. The method of claim 4 , wherein the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide contacts the sample at a temperature of about 20° C. to about 70° C.7. The method of claim 4 , wherein the carbodiimide has a pH of 8.0 in solution.8. A method for detecting a target short nucleic acid in a biological sample claim 4 , said method comprisinga) contacting the biological sample with an aldehyde-containing fixative;b) subsequently contacting the sample with a water- ...

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Номер записи: 59
23-05-2013 дата публикации

ZN (II) BASED COLORIMETRIC SENSOR AND PROCESS FOR THE PREPARATION THEREOF

Номер: US20130130306A1
Автор: Das Amitava, Ghosh Amrita, Mahato Prasenjit, Mishra Sandhya, Mishra Sanjiv Kumar, Shrivastava Anupama
Принадлежит: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH

A colorimetric chemosensor molecule having a aza-macrocycle Zn (II)-complex (L.Zn) (Scheme 1, Formula 1A) which can recognize selectively and efficiently ATP (Adenosine triphosphate), a biologically significant triphosphate in aqueous medium at pH 7.4 is described. Since ATP is the source of energy in living organisms, L.Zn (Scheme 1, Formula 1A) can also be used as a staining agent in the living cells through binding to ATP, generated in situ during the metabolic process. 2. (canceled)3. The compound as claimed in claim 1 , wherein said compound is a colorimetric sensor and useful as a viable staining agent for living cells.4. The compound as claimed in claim 3 , wherein said living cells are selected from Prokaryotic and Eukaryotic cells.5BacillusPseudomonas. The compound as claimed in claim 3 , wherein prokaryotic cells used are species and species.6Saccharomyces cerevisiae. The compound as claimed in claim 3 , wherein Eukaryotic cells used is yeast ().8. The process as claimed in step a) of claim 7 , wherein the solvent is selected from the group consisting of chloroform claim 7 , methanol claim 7 , dichloromethane and tetrahydrofuran (THF).9. The process as claimed in step i) of claim 7 , wherein alcohol is selected from methanol and ethanol.10. A process for staining living cells using the compound as claimed in claim 1 , comprising the steps of:{'i': Saccharomyces', 'Bacillus', 'Pseudomonas, "(a) cultivating (i) species (MTCC 5548) in glucose yeast extract agar (GYE) media, (ii) species (MTCC 5549) in Nutrient broth and (iii) species (MTCC 5550) in King's B medium at a temperature in the range of 30 to 40° C. for a period in the range of 6 to 36 hours;"}{'i': 'Saccharomyces', 'sup': −4', '−4, 'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(b) incubating the species (MTCC 5548) cells with 0.6×10to 1.2×10M compound of in the temperature range of 28 to 38° C. for a period in the range of 5 to 20 minutes;'}(c) fixing the incubated sample obtained in step (b) ...

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Номер записи: 60
23-05-2013 дата публикации

IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD

Номер: US20130130366A1
Автор: Angros Lee H.
Принадлежит:

An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide. 1. An automated apparatus for treating microscope slides , comprising:a slide support assembly comprising a first slide support element for supporting a first microscope slide and a second slide support element for supporting a second microscope slide, wherein the first slide support element and the second slide support element can be moved horizontally independently of each other and wherein the horizontal movement is in a single back and forth direction,wherein the first slide support element can be moved horizontally independently to a position for placement or removal of the first microscope slide on or from the first slide support element while the second slide support element is in a treatment position for treating the microscope slide thereon;a first heating element for heating the first microscope slide and a second heating element for heating the second microscope slide and wherein the first heating element can heat the first microscope slide independently of the heating of the second microscope slide by the second heating element; anda microprocessor programmed to separately control the first heating element and the second heating element.2. The automated apparatus of wherein the horizontal movement of the first slide support element and the second slide support element is a sliding ...

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Номер записи: 61
23-05-2013 дата публикации

Biological Sample Pretreatment Method and Apparatus

Номер: US20130130401A1
Автор: Ito Shinya, Kanda Katsuhiro, Nogami Makoto, Waki Izumi
Принадлежит:

A sample treatment apparatus is disclosed that allows a filtrate after reaction to be supplied to a solid phase extraction treatment without temporary collection. A necessary amount of a solid phase extracting agent is added to a solid phase extraction device for conditioning. An aqueous solution is added to the extracting agent and supernatants are discarded. A filter device is attached to an opening of the extraction and a sample is added into a filter reservoir. A hemolyzing agent is added to the sample, and they are stirred sufficiently to cause disruption of blood cells. A protein-denaturing solution is added to the mixture, and they stirred sufficiently to denature proteins contained in the sample. They are centrifuged to leave aggregate in the filter device, and the filtrate is supplied to the solid phase extracting agent through a filter unit without collecting the filtrate. 1. A method of pretreating a biological sample , comprising:supplying a biological sample and a deproteinizing reagent to a filter unit contained in a filter device;supplying a filtrate from the filter unit to a solid phase extracting agent, the solid phase extracting agent being contained in a solid phase extraction device, andextracting an aimed component in the biological sample from the filtrate contained in the solid phase extracting agent.2. The method of pretreating a biological sample according to claim 1 ,wherein the solid phase extraction device is a vessel, inserting the filter device into the solid phase extraction device;', 'supplying the biological sample and the deproteinizing reagent to the filter unit;, 'the method comprising the steps ofthereafter transferring the filtrate, from the filter device to the solid phase extracting device, by centrifugation; andsuspending the solid phase extracting agent, which is contained in the solid phase extraction device, in the filtrate transferred to the solid phase extracting device.3. The method of pretreating a biological sample ...

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Номер записи: 62
30-05-2013 дата публикации

One-Step Cell and Tissue Preservative for Morphologic and Molecular Analysis

Номер: US20130137094A1
Автор: Claudius Mueller, Lance A. Liotta, Virginia A. Espina
Принадлежит: George Mason Intellectual Properties Inc

The invention relates to a one-step chemical composition that preserves animal tissue, cells, and biomolecules, such as human tissue, human cells, and biomolecules therein. It improves the fidelity and morphologic structure of cells, organelles, and nuclear chromatin, and maintains and enhances the cellular antigenicity for immunohistochemistry and flow cytometry, while preserving proteins, post-translational modifications of proteins, and nucleic acids. In one embodiment, the composition comprises a) a non-aldehyde precipitating fixative at a concentration below 25% (volume/volume), b) a reversible/cleavable protein cross-linker that targets lipid-associated molecules, and c) a c reversible/cleavable protein cross-linker that targets water soluble molecules. In another embodiment, the composition further includes a kinase inhibitor, a phosphatase inhibitor, and a permeation enhancer. In still another embodiment, the compositions further include lactic acid at a concentration sufficient to maintain cellular nuclear volume at a level equivalent to aldehyde fixation of the same type of cell. In a further embodiment, the composition comprises: a) a precipitating fixative, b) a reversible/cleavable cross-linker, c) a permeation enhancer, d) a kinase inhibitor, e) a phosphatase inhibitor, and f) a carboxylic acid. In a still further embodiment, the invention comprises method for preserving a biological sample by contacting the sample with the composition of the invention under conditions effective for the preservation of the sample.

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Номер записи: 63
30-05-2013 дата публикации

System and method including analytical units

Номер: US20130137110A1
Автор: Charles S. Kraihanzel
Принадлежит: Beckman Coulter Inc

Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

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Номер записи: 64
30-05-2013 дата публикации

METHOD AND SYSTEM FOR AUTOMATED DEPARAFFINIZATION AND NON-IMMUNOHISTOCHEMICAL SPECIAL STAINING OF TISSUE SAMPLES

Номер: US20130137136A1
Автор: Beard Robin, Cobb Debra
Принадлежит:

Disclosed are methods and systems for automated deparaffinization and histochemical staining of tissue samples. Samples are automatically deparaffinized and stained without the use of harsh chemicals and without the use of extreme temperatures. 1. A method of deparaffinizing and performing special staining of paraffin embedded tissue samples comprising:a) loading a first microscope slide comprising at least one paraffin embedded tissue sample in a horizontal position onto an automated special stainer compromising a robotic reagent dispensing carousel;b) dispensing a covering volume of a non-buffered aqueous deparaffinization fluid comprising water and a water-miscible paraffin solvent onto said first microscope slide such that said tissue sample is covered by said deparaffinization fluid;c) automatically controlling the temperature of said first microscope slide to a programmed temperature of between about 50° C. and about 60° C. for a programmed time of less than about 11 minutes;d) robotically removing paraffin together with deparaffinization fluid from said tissue sample;e) dispensing a covering volume of clearing fluid onto said first microscope slide;f) automatically controlling temperature of said first microscope slide to a programmed temperature of between ambient and about 40° C. for a programmed time of less than about 4 minutes;g) robotically removing said clearing fluid from said first microscope slide; andh) staining said tissue sample by rotating said reagent dispensing carousel to a position above said tissue sample and dispensing a special staining reagent onto said tissue sample.2. The method of wherein said non-buffered aqueous deparaffinization fluid comprises about 2% 2-butoxyethanol.3. The method of wherein said clearing fluid comprises ethanol.4. The method of wherein said step of automatically removing paraffin together with deparaffinization fluid comprises robotically aspirating said deparaffinization fluid.5. The method of wherein said step ...

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Номер записи: 65
06-06-2013 дата публикации

Sample Container and Filtration Apparatus and Method of Filtration Using Same

Номер: US20130139617A1
Автор: Jordan George W., Kane Jeffrey, TAYLOR Thomas
Принадлежит: ROUSH LIFE SCIENCES, LLC

A sample container and filtration apparatus includes a sample container portion with an interior volume and a fluid path passing through a bottom wall. The sample container may also include a valve casing connected to and moveable relative to the sample collection portion between a first and second position. A valve secured to and moveable with the valve casing may close the fluid path of the sample collection portion when in a first position and open the fluid path when in a second position. The apparatus may also include a vacuum base coupling portion that is distal to the valve casing, and includes a filter membrane support surface for supporting a filter membrane. 1. A sample container comprising:a sample collection portion having at least one wall defining an interior volume of the sample container and an outlet passing through a bottom wall;a valve casing moveably connected to the sample collection portion in a telescopic manner, the sample collection portion and valve casing being moveable relative to one another between a first position and a second position, the valve casing forming a vacuum base coupling portion; anda valve secured to and moveable with the valve casing, the valve closing the outlet of the sample collection portion when in the first position and extending into the interior volume of the sample container when in the second position, the valve configured to open a pathway between the interior volume of the sample collection portion and an exterior when in the second position.2. A sample container according to further comprising a locking mechanism located between the sample container portion and the valve casing claim 1 , the locking mechanism preventing the relative movement between the sample collection portion and the valve casing.3. A sample container according to claim 2 , wherein the locking mechanism includes a peel strip claim 2 , the peel strip being removable from the sample container to allow the relative movement between the ...

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Номер записи: 66
06-06-2013 дата публикации

Pressure Monitoring Of Whole Blood Aspirations To Determine Completeness Of Whole Blood Mixing

Номер: US20130143257A1
Автор: Mizzer John P., Small Nathan A.
Принадлежит:

Methods for use with chemical analyzers aspirate a sample portion from one location to dispense it at a second location, aspirate another sample portion at that second location and dispense it at the first location, and measure the pressure values experienced inside a probe performing the aspirations and dispenses. By comparing the pressure values (or other values indicative of the viscosity or other relevant properties of the sample), the chemical analyzer can determine if the sample is sufficiently mixed or if the sample components remain separated and the method should be repeated. 1. A method of mixing a sample comprising the steps of:(a) aspirating a first portion of a sample to be mixed from a first level within a vessel containing the sample;(b) dispensing the first portion of the sample at a second level within the vessel;(c) measuring a first set of one or more values relating to at least one property of the first portion of the sample during at least one of the steps of aspirating or dispensing the first portion of the sample;(d) aspirating a second portion of the sample to be mixed from approximately the second level within the vessel;(e) dispensing the second portion of the sample at approximately the first level within the vessel;(f) measuring a second set of one or more values relating to the at least one property of the second portion of the sample during at least one of the steps of aspirating or dispensing the second portion of the sample;(g) comparing the first and second set of values to determine a difference of the at least one property of the first and second samples;(h) determining whether the difference of the at least one property of the first and second samples meets predetermined criteria; and(i) transferring at least a third portion of the sample to perform an assay in response to the determining step.2. The method of claim 1 , wherein the sample is whole blood.3. The method of claim 1 , wherein the sample is a fluid having components ...

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Номер записи: 67
13-06-2013 дата публикации

CELL CAPTURING FILTER HAVING HIGH ASPECT RATIO

Номер: US20130149217A1
Автор: BAEK Sang-hyun, JEONG Hyo-young, KIM Min-seoks, KIM Yeon-jeong, LEE Jeong-gun, Lee June-Young, OH Jin-mi, SIM Tae-seok, SONG Mi-jeong
Принадлежит: SAMSUNG ELECTRONICS CO., LTD.

A cell capturing filter, in which cells or particles having a predetermined size or greater in a sample may be easily captured, and clogging of a flow passage due to the captured cells or particles may be prevented, and stresses acting on the captured cells or particles may be reduced, having a first surface and a second substrate having a second surface that is bonded to the first surface, wherein the first substrate may include a flow passage formed in the first surface of the first substrate so that a sample flows in the flow passage, and a barrier that protrudes across the flow passage, and the second substrate may include a fine groove formed in an area on the second surface corresponding to the barrier, and a gap may exist between a surface of the barrier and the fine groove. 1. A filter comprising:an inlet configured to accept a sample;an outlet configured to expel the sample;a flow passage having a first surface and a second surface that face each other such that the sample flows between the first and second surfaces from the inlet to the outlet; anda filter unit disposed in the flow passage to capture target cells or target particles from the sample flowing through the flow passage,wherein the filter unit comprises a barrier protruding from the second surface toward the first surface to narrow the flow passage to a gap formed between the barrier and the first surface of the flow passage.2. The filter of claim 1 , wherein the gap is smaller than a size of the target cell or the target particle in the sample.3. The filter of claim 1 , wherein an aspect ratio of a width of the filter unit to the gap is at least 1 claim 1 ,000:1.4. The filter of claim 1 , wherein the barrier has a sidewall traversing the flow passage in a direction generally perpendicular to the direction of flow from the inlet to the outlet claim 1 , and the sidewall is inclined relative to the second surface.5. The filter of claim 1 , wherein the filter unit is disposed nearer to the outlet ...

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Номер записи: 68
13-06-2013 дата публикации

Methods, Reagents and Kits for Preservation of Nucleic Acids in Biological Samples

Номер: US20130149691A1
Автор: Haj-Ahmad Yousef
Принадлежит: Norgen Biotek Corp.

Provided is a nucleic acid preservative comprising at least one reducing agent, at least one chaotropic substance, at least one polyamine substance and at least one chelating agent and uses thereof, and a method for the preservation of nucleic acids in a biological sample. Further provided are kits for use in the preservation of nucleic acids in a biological sample, and more particularly, a blood sample. 1. A nucleic acid preservative comprising at least one reducing agent , at least one chaotropic substance , at least one polyamine substance and at least one chelating agent.2. The nucleic acid preservative according to claim 1 , wherein the reducing agent is glutathione and wherein the glutathione is present in an amount from about 10 mM to about 200 mM.3. The nucleic acid preservative according to claim 1 , wherein the chaotropic substance is a lithium salt and wherein the lithium salt is present in an amount from about 1 M to about 4 M.4. The nucleic acid preservative according to claim 3 , wherein the lithium salt is LiCl.5. The nucleic acid preservative according to claim 1 , wherein the chaotropic substance is a guanidium salt claim 1 , and wherein the guanidium salt is present in an amount from about 0.1 M to about 0.9 M.6. The nucleic acid preservative according to claim 5 , wherein the guanidium salt is guanidine hydrochloride.7. The nucleic acid preservative according to claim 1 , wherein the chaotropic substance is urea and wherein the urea is present in an amount from about 2 M to about 12 M.8. The nucleic acid preservative according to claim 1 , wherein the polyamine substance is spermidine and wherein the spermidine is present in an amount from about 10 μM to about 300 μM.9. The nucleic acid preservative according to claim 1 , wherein the polyamine substance is biuret and wherein the biuret is present in an amount of about 10 mM to about 100 mM.10. The nucleic acid preservative according to claim 1 , wherein the chelating agent is EDTA and wherein the ...

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Номер записи: 69
13-06-2013 дата публикации

METHOD OF LABELING SULFENIC ACID-CONTAINING PROTEINS AND PEPTIDES

Номер: US20130149732A1
Автор: Furdui Cristina M., Qian Jiang, Tsang Allen W.
Принадлежит:

A method of labeling a sulfenic acid (—SOH) group of a cysteine residue in a protein; or peptide, comprises contacting said protein or peptide with a beta-ketoester to covalently couple said beta-ketoester to said cysteine residue and form a beta-ketoester-labeled cysteine residue in said protein or peptide. 1. A method of labeling a sulfenic acid (—SOH) group of a cysteine residue in a protein or peptide , comprising:contacting said protein or peptide with a beta-ketoester to covalently couple said beta-ketoester to said cysteine residue and form a beta-ketoester-labeled cysteine residue in said protein or peptide.2. The method of claim 1 , further comprising the step of:detecting said beta-ketoester-labeled cysteine residue in said protein or peptide.3. The method of claim 1 , further comprising the step of coupling a detectable group claim 1 , or a protein or peptide binding ligand claim 1 , to said beta-ketoester.4. The method of claim 3 , further comprising the step of detecting said detectable group.5. The method of claim 1 , further comprising the step of cleaving said beta-ketoester-labeled cysteine residue to produce a 5-isoxazolone-labeled cysteine residue in said protein or peptide.6. The method of claim 5 , further comprising the step of detecting said 5-isoxazolone-labeled cysteine residue in said protein or peptide.7. The method of claim 1 , wherein said contacting step is carried by contacting said beta-ketoester to a live cell containing said protein or peptide.8. The method of claim 1 , wherein said protein or peptide is selected from the group consisting of enzymes claim 1 , receptors claim 1 , ion channels claim 1 , transcription factors claim 1 , hormones claim 1 , receptor ligands claim 1 , and enzyme substrates.10. A beta-ketoester for use in covalently labeling an oxidized sulfenic acid cysteine residue of a protein or peptide.11. The beta-ketoester of claim 10 , wherein said protein or peptide is selected from the group consisting of enzymes ...

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Номер записи: 70
13-06-2013 дата публикации

System and method for sorting cells

Номер: US20130149737A1
Автор: George E. Seidel, John L. Schenk, Lisa A. Herickhoff
Принадлежит: XY LLC

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

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Номер записи: 71
13-06-2013 дата публикации

Sample preparation system for an analytical system for determining a measured variable of a liquid sample

Номер: US20130149790A1
Автор: Knodler Matthias, Mennicken Guido, Springmann Maike, Steuerwald Ralf
Принадлежит: Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG

A sample preparation system for an analytical system for determining a measured variable of a sample liquid, comprising: a transport unit connected with a sample taking location via a first fluid conducting line; a sample collecting unit, which serves the analytical system as a staging area for automated removal of liquid samples, based on which the measured variable is determined; a filter unit arranged between the sample taking location and the transport unit; and at least one reservoir connected via a second fluid conducting line with the filter unit for providing a cleaning medium for cleaning the filter unit; wherein the transport unit is embodied to transport sample liquid from the sample taking location through the filter unit into the sample collecting unit. The cleaning medium includes an oxidizing agent. 114-. (canceled)15. A sample preparation system for an analytical system for determining a measured variable of a sample liquid , comprising:a first fluid conducting line;a second fluid connecting line;a transport unit connected with a sample taking location via said first fluid conducting line;a sample collecting unit, which serves the analytical system as a staging area for automated removal of liquid samples, based on which the measured variable is determined;a filter unit arranged between said sample taking location and said transport unit; andat least one reservoir connected via said second fluid conducting line with said filter unit for providing a cleaning medium for cleaning said filter unit, wherein:said transport unit is embodied to transport sample liquid from said sample taking location through said filter unit into said sample collecting unit; andthe cleaning medium comprises an oxidizing agent.16. The sample preparation system as claimed in claim 15 , further comprising:a first valve unit, by means of which a first section of said first fluid conducting line extending between said filter unit and said first valve unit can be connected ...

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Номер записи: 72
20-06-2013 дата публикации

TISSUE STAINING METHOD, TISSUE EVALUATION METHOD AND BIOSUBSTANCE DETECTION METHOD

Номер: US20130157895A1
Автор: Aimiya Takuji, Gonda Kohsuke, GOUDA Hideki, Nakano Yasushi, Ohuchi Noriaki, OKADA Hisatake, Takeda Motohiro
Принадлежит:

A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured. 1. A tissue staining method comprising:staining a tissue with a staining reagent comprising a particle holding plural phosphors, wherein a biosubstance-recognizing body is bound to the particle.2. The tissue staining method according to claim 1 , wherein.the particle holding plural phosphors contains the plural phosphors therein.3. The tissue staining method according to claim 1 , whereinthe phosphor is a fluorescent dye, a semiconductor particle, or a rare-earth element particle.4. The tissue staining method according claim 1 , whereinthe particle holding the plural phosphors has a particle diameter of 40 to 500 nm.5. A tissue evaluation method claim 1 , comprising:staining a tissue section with a staining reagent comprising a phosphor-holding particle which holds plural phosphors, wherein a biosubstance-recognizing body is hound to the phosphor-holding particle;counting the number of bright spots of fluorescence in the stained tissue section; andevaluating an expression level of a biosubstance corresponding to the biosubstance-recognizing body in the stained tissue section on the basis of the number of the counted bright spots.6. The tissue evaluation method according to claim 5 , comprising:measuring brightness of each bright spot in the stained tissue section;determining a brightness distribution on the basis of the number of the counted bright spots and the be of each bright spot;calculating the brightness per phosphor-holding particle on the basis ...

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Номер записи: 73
27-06-2013 дата публикации

METHODS AND COMPOSITIONS FOR PREPARING SAMPLES FOR IMMUNOSTAINING

Номер: US20130164760A1
Автор: Fischer Timothy J., Nelson Ramona R., Taylor Adriann J., Whitehead Clark M.
Принадлежит: TriPath Imaging, Inc.

Compositions and methods for preparing a sample for immunological staining are provided. Compositions include kits comprising a first solution comprising a surfactant and a second solution comprising a chaotropic agent. Methods comprise contacting a sample, such as cells or tissues, with a first solution comprising a surfactant and then contacting the sample with a second solution comprising a chaotropic agent. The method does not require extreme heat for antigen retrieval and therefore, maintains the cellular morphology of the sample. 1. A kit comprising:a) a first solution comprising a surfactant; andb) a second solution comprising a chaotropic agent.2. The kit of claim 1 , wherein said surfactant is an anionic surfactant.3. The kit of claim 2 , wherein said anionic surfactant is sodium dodecyl sulfate (SDS).4. The kit of claim 3 , wherein said first solution comprises about 0.1% SDS.5. The kit of claim 1 , wherein said chaotropic agent is a chaotropic salt.6. The kit of claim 5 , wherein said chaotropic salt is a thiocyanate or perchlorate.7. The kit of claim 6 , wherein said thiocyanate is guanidine thiocyanate.8. The kit of claim 7 , wherein said second solution comprises about 3M guanidine thiocyanate.9. The kit of claim 6 , wherein said perchlorate is lithium perchlorate.10. The kit of claim 9 , wherein said second solution comprises about 3M lithium perchlorate.11. The kit of claim 1 , wherein said second solution further comprises a weak surfactant.12. The kit of claim 11 , wherein said weak surfactant is nonyl phenoxypolyethoxylethanol (NP-40).13. The kit of claim 12 , wherein said second solution comprises about 0.1% NP-40.14. The kit of claim 1 , wherein said kit further comprises an antibody that specifically binds an antigen.15. The kit of claim 14 , wherein said antigen is a nuclear antigen.16. The kit of claim 14 , wherein said antigen is selected from the group consisting of MCM2 claim 14 , MCM7 claim 14 , p16 claim 14 , and Ki67.17. The kit of ...

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Номер записи: 74
27-06-2013 дата публикации

AUTOMATED SYSTEM AND METHOD OF PROCESSING BIOLOGICAL SPECIMENS

Номер: US20130164781A1
Автор: Lefebvre Gilles
Принадлежит: Sakura Finetek U.S.A., Inc.

An apparatus including at least one of a stainer module and a coverslipper module; an imaging module; a storage module; an automated transport module for transporting at least one slide between at least one of the stainer module and the coverslipper module, the imaging module and the storage module; and a controller. A method including processing at least one slide; determining whether an imaging module is available for imaging of a biological specimen on the at least one slide; transporting the at least one slide to the imaging module using an automated transport module; and transporting the at least one slide to a storage module using the automated transport module when it is determined that the imaging module is not available. A system including a processing module for processing at least one slide including a biological specimen thereon. A machine readable medium. 1. A method comprising:processing at least one slide having a biological specimen thereon;determining whether an imaging module is available for imaging of the biological specimen on the at least one slide;transporting the at least one slide to the imaging module using an automated transport module when it is determined that the imaging module is available; andtransporting the at least one slide to a storage module using the automated transport module when it is determined that the imaging module is not available.2. The method of claim 1 , wherein processing comprises staining the biological specimen on the at least one slide.3. The method of claim 1 , wherein processing comprises applying a coverslip to the at least one slide.4. The method of claim 1 , wherein the imaging module is a first imaging module and determining further comprises:determining whether a second imaging module is available; anddetermining whether a third imaging module is available when the first imaging module and the second imaging module are not available.5. The method of claim 4 , wherein transporting comprises transporting ...

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Номер записи: 75
04-07-2013 дата публикации

METHODS OF USING DYES IN ASSOCIATION WITH NUCLEIC ACID STAINING OR DETECTION AND ASSOCIATED TECHNOLOGY

Номер: US20130167309A1
Автор: Leung Wai-Yee, Mao Fei
Принадлежит: Biotium, Inc.

Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids. 1. A method of staining a sample that is exposed to a matrix or a surface , wherein if nucleic acid is present in the sample it is capable of being immobilized relative to the matrix or the surface , comprising:providing an aqueous solution comprising a fluorescent nucleic acid dye and a salt that comprises an anion that would be sufficient as a component of a strong acid and a cation that would be sufficient as a component of a strong base; andexposing the sample to the aqueous solution.2. The method of claim 1 , wherein a concentration of the salt is from about 5 mM to about 0.5 M claim 1 , inclusive.3. The method of claim 1 , wherein a concentration of the salt is about 0.05 M to about 0.2 M claim 1 , inclusive.4. The method of claim 1 , wherein the strong acid has a pKa of about 2 or less and the strong base has a pKa of about 10 or more.5. The method of claim 1 , wherein the salt is selected from NaCl claim 1 , sodium bromide claim 1 , sodium sulfate claim 1 , potassium chloride claim 1 , potassium bromide claim 1 , potassium sulfate claim 1 , magnesium chloride and tetramethylammonium chloride.6. The method of claim 1 , wherein the fluorescent nucleic acid dye is a monomeric dye or a dimeric dye. This application is ...

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Номер записи: 76
04-07-2013 дата публикации

Extraction Device and Method for Extracting Ingredients from a Biological Sample

Номер: US20130171629A1
Автор: Brand Wolfgang, Henn Karl, Looser Ralf, Mahler Sven
Принадлежит: BASF Plant Science Company GmbH

An extraction device () for extracting at least one ingredient from a biological sample () is disclosed, specifically for extracting metabolites from a plant specimen. The extraction device () has at least one liquid charger () which is adapted to be brought in contact with a first side () of the sample (). The liquid charger () is adapted to apply an extraction liquid (), specifically at least one solvent, to the first side () of the sample (). The extraction device () further has at least one sampling device () which is adapted to be brought in contact with a second side () of the sample (). The sampling device () is adapted to collect sample fluid () comprising the extraction liquid () and the ingredient. 125-. (canceled)26. A method for extracting at least one ingredient from a biological sample , wherein a liquid charger of an extraction device is brought in contact with a first side of the sample and wherein an extraction liquid is applied to the first side of the sample by using the liquid charger , wherein further at least one sampling device of the extraction device is used , wherein the sampling device is brought in contact with a second side of the sample and wherein a sample fluid comprising the extraction liquid and the ingredient is collected by using the sampling device , wherein the liquid charger and the sampling device are connected by at least one connection mechanism , wherein the connection mechanism is adapted to vary a distance between the liquid charger and the sampling device and wherein the liquid charger applies a clamping force to the sample located between the liquid charger and the sampling device , wherein the biological sample is taken from a plant and is selected from the group consisting of leaves , trunks , stems , roots , storage roots , branches , inflorescences , flowers , infructescences , fruits , ears , cobs and seeds , wherein the extraction device is clamped onto the biological sample and the extraction is performed without ...

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Номер записи: 77
04-07-2013 дата публикации

SOLID REAGENT DISSOLVING DEVICE AND METHOD OF DISSOLVING SOLID REAGENT BY USING THE SAME

Номер: US20130171640A1
Автор: Chi Sung-min, HWANG Kyu-youn, JUNG Sung-ouk, KIM Joon-ho, KWON Sung-hong
Принадлежит: SAMSUNG ELECTRONICS CO., LTD.

A solid reagent dissolving device including a flexible layer; an upper plate disposed on the flexible layer; and a lower plate disposed under the flexible layer, wherein the upper plate comprises a plurality of minute channels, a dissolution chamber connected with the plurality of minute channels, and a protrusion for limiting a flow of a fluid flowing through one of the plurality of minute channels, the lower plate comprises a plurality of penetration holes that correspond to the protrusion and the dissolution chamber, respectively, and one side of each of the plurality of penetration holes, the plurality of minute channels, and the dissolution chamber are covered with the flexible layer, and method of using same. 1. A solid reagent dissolving device comprising:a lower plate;a flexible layer disposed on the lower plate; and a plurality of channels;', 'a dissolution chamber in fluid communication with the plurality of channels; and', 'at least one protrusion that limits flow of fluid through at least one of the plurality of channels;, 'an upper plate disposed on the flexible layer, wherein the upper plate comprises'}wherein the lower plate comprises a plurality of penetration holes in regions of the lower plate that correspond to the protrusion and the dissolution chamber of the upper plate, respectively; andwherein the flexible layer covers each of the plurality of penetration holes, the plurality of channels, and the dissolution chamber.2. The solid reagent dissolving device of claim 1 , further comprising a cover positioned on the upper plate and covering at least a portion of the dissolution chamber.3. The solid reagent dissolving device of claim 1 , wherein a portion of the upper plate corresponding to the dissolution chamber is parallel with the flexible layer.4. The solid reagent dissolving device of claim 1 , wherein each penetration hole comprises an opening in an upper side of the lower plate and an opening on a lower side of the lower plate claim 1 , ...

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Номер записи: 78
11-07-2013 дата публикации

METHOD FOR PREPARATION OF ALGAL CELLS, AND KIT FOR EVALUATION OF TOXICITY OF CHEMICAL SUBSTANCE

Номер: US20130177967A1
Автор: Katsumata Masakazu, Kazumura Kimiko, Takeuchi Ayano
Принадлежит: HAMAMATSU PHOTONICS K.K.

The present invention provides a method for preparing algal cells used for evaluating a toxicity of a chemical substance by delayed luminescence, the method comprising the step of freezing algal cells in a logarithmic growth phase. 1. A method for preparing algal cells used for evaluating a toxicity of a chemical substance by delayed luminescence , the method comprising the step of freezing algal cells in a logarithmic growth phase.2. A method according to claim 1 , wherein at least 50% of the total number of the algal cells to be frozen have a particle size of 4.5 μm or greater.3. A method according to claim 1 , wherein the algal cells to be frozen exhibit a bimodal particle size distribution curve.4. A method according to claim 3 , wherein the algal cells to be frozen are green algae (Pseudokirchneriella subcapitata) preserved as strain No. NIES-35 in National Institute for Environmental Studies; andwherein at least 55% of the total number of the algal cells to be frozen have a particle size not smaller than that at a valley value in the bimodal particle size distribution curve.5. A method according to claim 3 , wherein the algal cells to be frozen are green algae (Pseudokirchneriella subcapitata) preserved as strain No. NIES-35 in National Institute for Environmental Studies; andwherein the number of cells at the larger-particle-size peak in the bimodal particle size distribution curve is at least 50% of the total of the number of cells at the smaller-particle-size peak and the number of the cells at the larger-particle-size peak.6. A method according to claim 3 , wherein the algal cells to be frozen are green algae (Pseudokirchneriella subcapitata) preserved as ATCC No. 22662 in the American Type Culture Collection; andwherein at least 46% of the total number of the algal cells to be frozen have a particle size not smaller than that at a valley value in the bimodal particle size distribution curve.7. A method according to claim 3 , wherein the algal cells to be ...

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Номер записи: 79
11-07-2013 дата публикации

Accelerated Wright-Giemsa and May-Grunwald Staining Methods

Номер: US20130177974A1
Автор: Alicea Rene Nieves, Chacko Koshy T., Mamaghani Abe S., Rao Rupa
Принадлежит: ABBOTT LABORATORIES

The present disclosure provides methods for carrying out Romanowsky-type stains, specifically Wright-Giemsa and May-Grúnwald stains, quickly and efficiently. The methods greatly reduce the overall amount of time required to complete a Wright-Giemsa stain or a May-Grúnwald stain of sufficient quality on a biological sample. The subject methods can be applied to both manual and automated staining procedures. 1. An automated staining system for performing a Wright-Giemsa stain on one or more samples , comprising:a sample application subsystem that applies a sample to a substrate;a fixation reagent bath;a Wright-Giemsa staining reagent bath;a rinse reagent bath;a sample transfer subsystem for moving the substrate from the sample application subsystem, to the fixation reagent bath, to the Wright-Giemsa staining bath, and then to the rinse reagent bath; anda computer readable storage medium comprising instructions executable by at least one processing device that, when executed, cause the processing device to control the sample transfer subsystem such that the sample transfer subsystem places the one or more samplesin the fixation reagent bath for a period of time ranging from about 15 seconds up to about 45 seconds,in the Wright-Giemsa staining reagent bath for a period of time ranging from about 15 seconds up to about 60 seconds, andin the rinse reagent bath for a period of time ranging from about 105 seconds up to about 135 seconds.2. The system of claim 1 , wherein the sample is placed in the fixation reagent bath for a period of time ranging from about 20 seconds up to about 40 seconds.3. The system of claim 1 , wherein the sample is placed in the fixation reagent bath for a period of time ranging from about 25 seconds up to about 35 seconds.4. The system of claim 1 , wherein the fixation reagent is methanol.5. The system of claim 1 , wherein the sample is placed in the Wright-Giemsa staining reagent bath for a period of time ranging from about 20 seconds up to about ...

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Номер записи: 80
11-07-2013 дата публикации

TREATMENT FOR STABILIZING NUCLEIC ACID ARRAYS

Номер: US20130178369A1
Автор: Burns Norman Lee, Shafto Jay Willis
Принадлежит: Complete Genomics, Inc.

The present invention is directed to treatment of nucleic acid molecules that are attached or associated with solid supports for biochemical analysis, including nucleic acid sequencing. After loading on the solid support, the nucleic acid molecules are treated with a composition comprising a condensing agent, a volume excluding agent, or both, then treated with a composition comprising a protein. 1. A method of treating a nucleic acid array to improve stability of the array during nucleic acid analysis , the method comprising:(a) providing a nucleic acid array comprising (i) a support comprising a surface and (ii) nucleic acid molecules attached to the surface;(b) condensing the nucleic acid molecules attached to the surface, thereby producing condensed nucleic acid molecules; and(c) coating the condensed nucleic acid molecules with a protein.2. The method of wherein said nucleic acid analysis comprises nucleic acid sequencing claim 1 , nucleic acid hybridization assay claim 1 , or an enzymatically assisted nucleic acid assay.3. The method of wherein said step of condensing comprises contacting the array with a composition comprising a nucleic acid condensing agent claim 1 , a volume excluding agent claim 1 , or both a nucleic acid condensing agent and a volume excluding agent.4. The method of wherein the nucleic acid condensing agent is an alcohol.5. The method of wherein the alcohol is isopropyl alcohol.6. The method of wherein the volume exclusion agent is polyethylene glycol.7. The method of comprising coating the nucleic acid molecules with composition comprising a protein that binds to the surface and does not interfere with the nucleic acid analysis.8. The method of wherein the protein is partially denatured.9. The method of wherein the protein is a serum albumin.10. The method of wherein the protein is bovine serum albumin or human serum albumin.11. The method of wherein the nucleic acid is selected from the group consisting of DNA and RNA.12. The method of ...

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Номер записи: 81
11-07-2013 дата публикации

METHODS OF ANALYZING AN H&E STAINED BIOLOGICAL SAMPLE

Номер: US20130178392A1
Автор: Kaanumalle Lakshmi Sireesha, Kenny Kevin Bernard, Natarajan Arunkumar, Sood Anup
Принадлежит: GENERAL ELECTRIC COMPANY

Methods comprising the use probing multiple targets in a H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, observing the sample, removing the hematoxylin and partially removing the eosin by washing the sample, contacting the sample with a borate salt, and irradiating the sample to remove the residual eosin fluorescence. The method further includes the optionally performing the additional steps of binding at least one probe to one or more targets to the sample ,observing a signal from the probe and contacting the sample with a bleaching agent. The process of binding, observing and bleaching may be iteratively repeated. 1. A method of analyzing a hematoxylin and eosin stained biological sample comprising:(a) providing a hematoxylin and eosin stained biological sample containing multiple targets;(b) observing the sample;(c) removing the hematoxylin and partially removing the eosin by washing the sample;(d) contacting the sample with an electron transfer reagent(e) irradiating the sample of step (d) to remove residual fluorescence.2. The method of claim 1 , wherein irradiating the sample in step (e) is accomplished by exposing the sample to light of 350 nm-1.3 μM in wavelength.3. The method of claim 2 , wherein irradiating the sample in step (e) is accomplished by exposing the sample to light of 400-700 nm in wavelength.4. The method of claim 1 , wherein irradiating the sample in (e) is carried out in the presence of a buffer at pH of 5-9.5. The method of wherein step (e) is carried out at the temperature of 4-50° C.6. The method of claim 1 , wherein step (e) is performed for about 20 seconds to about 15 minutes.7. The method of claim 1 , wherein the eosin is irreversibly modified.8. The method of claim 1 , wherein no detectable signal is observed after step (e).9. The method according to wherein the electron transfer reagent is a borate salt.11. The ...

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Номер записи: 82
18-07-2013 дата публикации

Interactive and automated tissue image analysis with global training database and variable-abstraction processing in cytological specimen classification and laser capture microdissection applications

Номер: US20130182922A1
Автор: David H. Kil
Принадлежит: Life Technologies Corp

A system and method for performing tissue image analysis and region of interest identification for further processing applications such as laser capture microdissection is provided. The invention provides three-stage processing with flexible state transition that allows image recognition to be performed at an appropriate level of abstraction. The three stages include processing at one or more than one of the pixel, subimage and object levels of processing. Also, the invention provides both an interactive mode and a high-throughput batch mode which employs training files generated automatically.

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Номер записи: 83
18-07-2013 дата публикации

LOW RESOURCE PROCESSOR USING SURFACE TENSION VALVES FOR EXTRACTING, CONCENTRATING AND DETECTING MOLECULAR SPECIES

Номер: US20130183678A1
Автор: Haselton Rick
Принадлежит: VANDERBILT UNIVERSITY

Systems and methods are described for isolation, separation and detection of a molecular species using a low resource device for processing of samples. Methods include isolation, separation and detection of a molecular species for protein-protein, DNA-DNA and other chemical interactions. 1. A method of processing a sample comprising:(a) providing a device comprising a plurality of sequential chambers connected by tubing, each of said sequential chambers comprising a fluid and separated by gas-based surface tension valves, wherein a first reaction chamber comprises a particle having a reactant on its surface;(b) introducing into said first reaction chamber a sample;(c) incubating said first reaction chamber under conditions sufficient to permit reaction of said reactant with an analyte in said sample;(d) transporting said particle from said first reaction chamber into at least a second chamber through tubing disposed therebetween; and(e) detecting interaction of said analyte with said reactant.2. The method of claim 1 , wherein said device comprises at least three chambers.3. The method of claim 2 , wherein said device comprises said first reaction chamber claim 2 , a first processing chamber and a first detection chamber claim 2 , wherein said first processing chamber is disposed between said first reaction chamber and said first action chamber.4. The method of claim 2 , further comprising reversing the transport of said particle to reintroduce said particle into a chamber through which it has already passed.5. The method of claim 1 , wherein said device comprises continuous tubing and surface tension valves separating said tubing into said plurality of chambers.6. (canceled)7. The method of claim 5 , wherein said tubing comprises an inner surface coated by a polymer.8. The method of claim 1 , wherein said particle is a magnetic particle claim 1 , a paramagnetic particle or a non-magnetic particle having a density of >1 or <1.9. The method of claim 8 , wherein ...

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Номер записи: 84
18-07-2013 дата публикации

APPARATUS FOR EXECUTION OF TREATMENT OPERATIONS IN CONNECTION WITH COLOURING OF TISSUE SPECIMENS ON OBJECT GLASSES

Номер: US20130183708A1
Автор: Ljungmann Oystein, LJUNGMANN Torstein
Принадлежит: Dako Instrumec AS

An apparatus for automatic execution of different treatment operations in connection with staining of tissue specimens on microscope slides, wherein the apparatus () comprises a loading station () for microscope slides () with tissue specimens, a number of reagent stations () for staining of the tissue specimens on supp Ned microscope slides, a conveyor () for transfer of microscope slides () between the stations () in accordance with a staining program, an unloading station () for treated microscope slides, and a control unit () for controlling the treatment operations in accordance with a data program, The apparatus comprise a photo station () with a digital camera () for automatic photographing with a background light source of the finished treated tissue specimens on supplied microscope slides (). The camera is connected to the control unit () of the apparatus and the control unit is arranged to store the picture of each individual tissue specimen and information which is located on the relevant microscopic slide () and preferably also information about the staining program and status of reagents at the reagent stations () for automatic transmission of the information to a place for result analysis. 118-. (canceled)19. An automated stainer comprising multiple stations for staining of tissue specimens on microscope slides , the multiple stations comprising:a loading station configured to load the microscope slides on the apparatus;two or more staining stations configured to stain the tissue specimens on the microscope slides;a sealing station configured to apply adhesives or films on the microscope slides; anda photo station comprising a camera mounted on a microscope, wherein the photo station is fitted with a background light source to take one or more back lit photomicrographs of the stained tissue specimens on the microscope slides.20. The automated stainer of claim 19 , wherein the sealing station is also configured to apply a cover glass.21. The automated ...

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Номер записи: 85
18-07-2013 дата публикации

Cell processing apparatus, sample preparation apparatus, and cell analyzer

Номер: US20130183747A1
Автор: Hironori Kobayashi, Junyi Ding, Koki Tajima, Masakazu Fukuda, Ryuichiro Ebi
Принадлежит: Sysmex Corp

A cell processing apparatus includes a storage container that contains liquid L including a biological sample; a filter that prevents a first cell C 1 in the biological sample from passing and allows a second cell C 2 having a smaller diameter than that of the first cell C 1 to pass; and a filtration cylinder for separating, in the storage container and via the filter, the liquid L into a first liquid L 1 mainly including the first cell C 1 and a second liquid L 2 mainly including the second cell C 2 . A measurement target cell filtered by the filter among other cells can be easily collected.

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Номер записи: 86
25-07-2013 дата публикации

POLYMER STABILIZATION OF CHROMOGEN SOLUTIONS

Номер: US20130189680A1
Автор: Bieniarz Christopher, Kelly Brian D., Kosmeder Jerome W., May Eric J., Morrison Larry
Принадлежит: Ventana Medical Systems, Inc.

Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit. 2. The composition of claim 1 , wherein the polymer comprises a sulfate functional group claim 1 , a sulfonate functional group claim 1 , an amine functional groups claim 1 , or combinations thereof.3. The composition of claim 2 , wherein the amine functional groups is a primary amine claim 2 , a secondary amine claim 2 , a tertiary amine claim 2 , or a quaternary amine.4. The composition of claim 1 , wherein the polymer is a polyalkyleneamine polymer.5. The composition of claim 1 , wherein the polymer is selected from dextran sulfate claim 1 , polystyrene sulfonate claim 1 , polyethyleneimine claim 1 , aminodextran claim 1 , dextran DEAE claim 1 , polystyrene sulfonate maleic acid co-polymer claim 1 , polyvinylsulfonate claim 1 , poly(2-vinyl-1-methylpyridinium bromide claim 1 , poly(2-methylacryloxethyltrimethylammonium bromide claim 1 , poly(acrylamide/2-methylacryloxethyltrimethylammonium bromide claim 1 , and combinations thereof.6. The composition of claim 1 , wherein the polymer is a linear or branched polyethyleneimine.7. The composition of claim 6 , wherein the linear or branched polyethyleneimine is a 4 kDa HCl salt of polyethyleneimine.8. The composition of claim 1 , wherein the polymer has a number average molecular weight of between about 1 kDa and about 500 kDa.9. The composition of claim 1 , wherein the polymer is about 0.05 percent to about 10 percent of the solution by weight.10. The composition of claim 1 , wherein the DAB chromogen or the derivative thereof has a concentration of about 0.1 mM to about 100 mM.13. The composition of claim 1 , further comprising an enhancer selected from the group consisting of imidazole ...

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Номер записи: 87
25-07-2013 дата публикации

BREAKAGE OF AN EMULSION CONTAINING NUCLEIC ACID

Номер: US20130189700A1
Автор: Hindson Benjamin J., Lucero Michael Y., Saxonov Serge, So Austin P., Tzonev Svilen S.
Принадлежит: Bio-Rad Laboratories, Inc.

Methods of processing an emulsion of aqueous droplets containing nucleic acid. The methods may include breakage of the emulsion with a destabilizing fluid including a halogen-substituted hydrocarbon. 1. A method of processing an emulsion , comprising:providing an emulsion of aqueous droplets containing nucleic acid;mixing the emulsion with of an amount of destabilizing fluid effective to break the emulsion, the destabilizing fluid including a halogen-substituted hydrocarbon; andisolating the nucleic acid after mixing.2. The method of claim 1 , wherein the destabilizing fluid includes chloroform.3. The method of claim 1 , wherein the destabilizing fluid is composed at least predominantly of chloroform.4. The method of claim 1 , further comprising a step of performing a sequencing reaction with the isolated nucleic acid.5. The method of claim 1 , wherein the droplets contain amplified nucleic acid connected to beads claim 1 , and wherein the step of isolating is performed with the amplified nucleic acid connected to the beads.6. The method of claim 1 , wherein the step of isolating nucleic acid includes a step of isolating at least a portion of a continuous aqueous phase formed by coalescence of the droplets containing nucleic acid.7. The method of claim 1 , further comprising a step of centrifuging the emulsion mixed with destabilizing fluid.8. The method of claim 7 , wherein a container holds the emulsion mixed with stabilizing fluid during the step of centrifuging claim 7 , and wherein the step of isolating includes a step of removing aqueous fluid claim 7 , selectively relative to beads to which the nucleic acid is connected claim 7 , from the container after the step of centrifuging.9. The method of claim 1 , further comprising a step of creating contact between the emulsion and the destabilizing fluid claim 1 , wherein the step of mixing includes a step of vortexing and/or shaking the emulsion and the destabilizing fluid after the step of creating contact.10. ...

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Номер записи: 88
25-07-2013 дата публикации

Sample analyzer and a sample analyzing method

Номер: US20130189708A1
Автор: Masaki Shiba, Tomoyuki Nishida, Yuji Wakamiya
Принадлежит: Sysmex Corp

Herewith disclosed is a sample analyzer comprising: a measurement section configured to perform a measurement on a sample and generate a measurement value according to the concentration of an analyte in the sample; a memory storing a calibration curve; an analysis section; an output section; and an instruction receiver. When the instruction receiver receives an instruction to perform a diluting measurement on a calibration sample, the measurement section dilutes the calibration sample by a predetermined ratio and performs a measurement on the diluted calibration sample, and the analysis section determines the concentration of the analyte in the diluted calibration sample by applying a measurement value obtained from the diluted calibration sample to the calibration curve. Information generated based on the determined concentration and the known concentration is output.

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Номер записи: 89
25-07-2013 дата публикации

METHOD FOR PREPARING AND/OR PROCESSING A BIOLOGICAL SAMPLE USING A MALODOUR COUNTERACTANT

Номер: US20130189726A1
Автор: Hiesinger Margit, Mueller Markus
Принадлежит: QIAGEN GmbH

The present invention pertains to a biotechnological method for preparing and/or processing a biological sample, in particular for isolating at least one target biomolecule therefrom, which characterised in that at least one malodour counteractant is used for preventing, reducing, masking and/or suppressing malodour and/or malodour formation during the preparation and/or processing of said biological sample. 118.-. (canceled)19. A method for preparing or processing a biological sample , comprising:(A) preparing or processing a biological sample, wherein at least one malodour counteractant is used for preventing, reducing, masking, or suppressing (i) malodour, (ii) malodour formation during the preparation or processing of said biological sample, or (iii) both (i) and (ii).20. The method of claim 19 , wherein step (A) comprises isolating at least one target biomolecule from said biological sample.21. The method of claim 19 , wherein the sample is selected from the group consisting of eukaryotic cells claim 19 , prokaryotic cells claim 19 , fungi claim 19 , cell cultures claim 19 , stool claim 19 , feces claim 19 , blood claim 19 , plasma claim 19 , serum claim 19 , body fluids claim 19 , body excretions claim 19 , saliva claim 19 , urine claim 19 , swabs claim 19 , tissue claim 19 , clinical samples claim 19 , and samples derived therefrom.22. The method of claim 19 , wherein step (A) is selected from the group consisting of cell culturing claim 19 , sample lysis claim 19 , isolation of biomolecules claim 19 , nucleic acid purification claim 19 , protein denaturation claim 19 , protein purification claim 19 , isolation of metabolites claim 19 , and isolation of components other than metabolites that are contained in the sample.23. The method of claim 19 , wherein the sample is selected from the group consisting of eukaryotic cells claim 19 , prokaryotic cells claim 19 , fungi claim 19 , cell cultures claim 19 , stool claim 19 , feces claim 19 , blood claim 19 , ...

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Номер записи: 90
25-07-2013 дата публикации

FIXATIVE AND STAINING SOLUTIONS

Номер: US20130189729A1
Автор: Lapen Daniel, Licari Kerri
Принадлежит: CONSTITUTION MEDICAL, INC.

The formulations, systems, and methods disclosed herein permit automated preparation of specimens (e.g., biological specimens) for examination. The disclosed formulations, systems, and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation phases for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining specimens. 1. A cytological staining solution , comprisingAzure B;methylene blue;a buffering agent;a surfactant,sodium chloride, andwater.2. The cytological staining solution of claim 1 , further comprising an antimicrobial agent.3. The cytological staining solution of claim 2 , wherein the solution comprises from about 0.2 to about 50 ppm of the antimicrobial agent.4. The cytological staining solution of claim 2 , wherein the antimicrobial agent is selected from the group consisting of benzalkonium chloride claim 2 , 5-chloro-2-methyl-4-isothiazolin-3-one claim 2 , 2-methyl-4-isothiazolin-3-one claim 2 , ProClin® claim 2 , azides claim 2 , merthiolates claim 2 , antibiotics claim 2 , and any combination thereof.540. The cytological staining solution of claim 2 , wherein the antimicrobial agent comprises 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one. 41. The cytological staining solution of claim claim 2 , wherein the antimicrobial agent is ProClin® 300.6. The cytological staining solution of claim 2 , further comprising acetic acid. 43. The cytological staining solution of claim 2 , wherein the solution comprises from about 0.25 to about one g/L azure B.7. The cytological staining ...

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Номер записи: 91
01-08-2013 дата публикации

SLIDE POCKET

Номер: US20130196339A1
Автор: Dowling James L., Nolet Christopher J., Von Bueren Erico, Yeaton Eric D.
Принадлежит: THERMO SHANDON LIMITED

A slide pocket includes a first shell portion having a slide well with a depth suitable for accepting a sample-containing portion of a slide thereby positioning a sample affixed to the sample-containing portion of the slide within the slide well. The slide well has a plurality of beveled interior walls, the beveled portions functioning to prevent the sample affixed to the sample-containing portion of the slide from contacting any surface of the slide well during insertion. The slide pocket has a second shell portion, mateable in fluid tight engagement with the first shell portion. The second shell portion has a slide well. The first and second shell portions engage to define a single continuous slide chamber, a fluid input channel and fluid input port in communication with the single continuous slide chamber, and a fluid output channel and fluid output port in communication with the single continuous slide chamber. 1) A slide pocket comprising: 'i) a slide well having a depth suitable for accepting the insertion of a sample-containing portion of a slide thereby positioning a sample affixed to the sample-containing portion of the slide within the slide well, the slide well having a first plurality of beveled interior walls, or wall faces, the beveled portions functioning to prevent the sample affixed to the sample-containing portion of the slide from contacting any surface of the slide well during insertion;', 'a) a first shell portion comprising 'i) a slide well having a depth suitable for accepting the insertion of a portion of the slide of element a) that is not a sample-containing portion of the slide;', 'b) a second shell portion, mateable in fluid tight engagement with the first shell portion, the second shell portion comprisingwherein the first and second shell portions, when in mated configuration, further comprise:c) a single continuous slide chamber defined by the slide wells of the first and second shell portions;d) a fluid input channel and fluid input ...

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Номер записи: 92
01-08-2013 дата публикации

FIXATIVE AND STAINING SOLUTIONS

Номер: US20130196370A1
Автор: Lapen Daniel, Licari Kerri
Принадлежит: CONSTITUTION MEDICAL, INC.

The formulations, systems, and methods disclosed herein permit automated preparation of specimens (e.g., biological specimens) for examination. The disclosed formulations, systems, and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation phases for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining specimens. 1. A cytological fixative solution containing a single stain , comprisinga single blue stain;a surfactant;an alcohol; andan agent selected from the group consisting of ethylene glycol, propylene glycol, polyethylene glycol, and any combination thereof,wherein the cytological fixative solution is substantially free of red dyes.2. The cytological fixative solution of claim 1 , wherein the blue stain is a thiazine dye.3. The cytological fixative solution of claim 1 , wherein the blue stain is Azure B.4. The cytological fixative solution of claim 1 , wherein the blue stain is present in an amount of from about 0.5 to about five g/L.5. The cytological fixative solution of claim 1 , wherein the surfactant is present in an amount of from about 0.05% to about 0.5% by volume.6. The cytological fixative solution of claim 1 , wherein the surfactant is present in an amount of from about 0.05% to about 0.3% by volume.7. The cytological fixative solution of claim 1 , wherein the surfactant is selected from the group consisting of non-ionic claim 1 , cationic claim 1 , anionic claim 1 , and zwitterionic surfactants.8. The cytological fixative solution of claim 7 , wherein the non-ionic surfactant is polysorbate ...

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Номер записи: 93
01-08-2013 дата публикации

TISSUE CASSETTE ASSEMBLY

Номер: US20130196371A1
Автор: Cooper Nathanael J., Freeland Jennifer H., Merati Parviz, Whittlinger Keith O.
Принадлежит:

A tissue cassette includes a body having a first floor and a first perimeter wall encircling and extending from the first floor so that the first floor and first perimeter wall at least partially bound a body cavity, the first floor having a plurality of spaced apart openings extending therethrough. A mold has a second floor and a second perimeter wall encircling and extending from the second floor so that the second floor and second perimeter wall at least partially bound a mold cavity, the second floor having at least one micropore extending therethrough, the at least one micropore having a maximum diameter less than 0.040 inches. A hinge couples the body to the mold so that the body and mold can move between a closed position wherein the first floor overlies the second floor so as to form a tissue compartment therebetween and an open position. 1. A tissue cassette comprising:a body comprising a first floor and a first perimeter wall encircling and extending from the first floor so that the first floor and first perimeter wall at least partially bound a body cavity, the first floor having a plurality of spaced apart openings extending therethrough;a mold comprising a second floor and a second perimeter wall encircling and extending from the second floor so that the second floor and second perimeter wall at least partially bound a mold cavity, the second floor having at least one micropore extending therethrough, the at least one micropore having a maximum diameter less than 0.040 inches; anda hinge coupling the body to the mold so that the body and mold can move between a closed position wherein the first floor overlies the second floor so as to form a tissue compartment therebetween and a open position.2. The tissue cassette as recited in claim 1 , wherein the at least one micropore has a diameter less than 0.040 inches.3. The tissue cassette as recited in claim 1 , wherein the at least one micropore comprises a plurality of spaced apart micropores in a range ...

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Номер записи: 94
01-08-2013 дата публикации

MICROFLUIDIC ELEMENT FOR THOROUGHLY MIXING A LIQUID WITH A REAGENT

Номер: US20130196447A1
Автор: Boehm Christoph
Принадлежит: ROCHE DIAGNOSTICS OPERATIONS, INC.

A microfluidic element for thoroughly mixing a liquid with a reagent used for the analysis of the liquid for an analyte contained therein and a method thereof are disclosed. The microfluidic element has a substrate and a channel structure. The channel structure includes an elongate mixing channel and an output channel. The mixing channel has an inlet opening and an outlet opening, and is implemented to mix the reagent contained therein with the liquid flowing through the inlet opening into the mixing channel. The outlet opening of the mixing channel is in fluid communication to the output channel. The outlet opening is positioned closer to the middle of the length of the mixing channel than the inlet opening. 1. A method for providing a homogeneous thoroughly mixed liquid comprising:providing a microfluidic element having a substrate and a channel structure, wherein the channel structure includes an elongate mixing channel and an output channel, wherein the mixing channel has an inlet opening and an outlet opening in fluid communication with the output channel, wherein the outlet opening is located closer to the middle of the length of the mixing channel than the inlet opening, and, wherein the mixing channel has a feed section between the inlet opening and the outlet opening and a complementary section, downstream from the outlet opening in the flow direction and opposite to the inlet opening;allowing a flow of liquid through the inlet opening into the mixing channel;dissolving a reagent contained in the mixing channel;exerting a force on the liquid in the mixing channel;allowing the liquid to flow into the output channel through the outlet opening of the mixing channel so that thorough mixing of the liquid and the reagent occur; andflowing partial volumes from the feed section and the complementary section of the mixing channel through the outlet opening into the output channel such that mixing of the two liquid partial volumes is supported by exertion of the ...

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Номер записи: 95
08-08-2013 дата публикации

SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS

Номер: US20130203046A1
Автор: WILTSIE Joshua D.
Принадлежит: Beckman Coulter, Inc.

Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. 191.-. (canceled)92. A method for mixing the contents of a well , comprising: 'wherein the segment of the endwall extends towards the center of the well at an angle relative to the vertical axis and has a radius about a mid-plane to create a culvert, the mid-plane being defined by the first sidewall and the second sidewall and;', 'directing a pipettor to a first location in an assay cartridge having a well with an endwall comprising a segment, a first sidewall, and a second sidewall;'}dispensing a liquid from the pipettor onto the culvert of the well, wherein the radius of the culvert collects the dispensed liquid and directs the dispensed liquid towards the midline of the culvert such that turbulence is induced in the flow of the dispensed liquid.93. The method of claim 92 , wherein the pipettor is directed to a second location within the well to collect the dispensed liquid claim 92 , followed by re-dispensing the collected liquid onto the culvert of the well.94. The method of claim 92 , wherein the radius of the culvert decreases in the direction of liquid flow claim 92 , thereby forming a funnel-like claim 92 , frusto-conical segment ...

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Номер записи: 96
08-08-2013 дата публикации

APPARATUS FOR PROCESSING BIOLOGICAL SAMPLES AND METHOD THEREOF

Номер: US20130203072A1
Автор: Ji Lingyun, Tian Feng, Zou Daniel Liang
Принадлежит:

The present invention provides devices, apparatuses and methods for automated processing of biological samples. This invention provides automated devices and automated methods of sequentially treating a biological sample with processing fluids in more than one washing basin. In some embodiments, the invention provides automated devices and automated methods of western blot processing. In some embodiments, the invention provides automated devices and automated methods of Southern blot processing. In some embodiments, the invention provides automated devices and automated methods of northern blot processing. In some embodiments, the invention provides automated devices and automated methods of staining biomolecules on solid supports. In some embodiments, the invention provides automated devices and automated methods of nucleic acid separation and isolation. 1. An automated bioprocessing device comprising:a) a housing;b) a cover;c) one or more sample holders, said sample holder holds one or more biological samples;{'b': '2', 'd) one or more removable washing basin assemblies on said housing, each washing basin assembly comprising at least washing basins, each washing basin configured to store a processing fluid, each washing basin configured to receive said sample holder, each washing basin configured to be of the same or different volume;'}e) one or more robot assemblies on said housing, each robot assembly configured to transport said sample holder and said biological samples therein from one said washing basin to another said washing basin, and to agitate said biological samples within said washing basin; andf) a control system configured to operate said robot assembly according to a predetermined schedule, said control system comprising at least one bioprocessing controller.2. The device of claim 1 , wherein each said removable washing basin assembly further comprises from 9 to 20 said washing basins claim 1 , said washing basins arranged in an array selected from ...

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Номер записи: 97
08-08-2013 дата публикации

Device and Method for Transporting and Preparing Cellular Material

Номер: US20130203102A1
Автор: Bernes Otto, Fiac Vass László
Принадлежит:

Embodiments of the invention relate to a device for transporting and preparing cellular material in a fluid for subsequent diagnostic purposes, comprising a cylinder having a fluid-tight screw-on cap and a fluid-tight base part that can be screwed off of the cylinder. The cap can be screwed onto the base part in a fluid-tight matter. The device provides for introducing cellular material to be diagnosed into a fluid in the cylinder, closing the cylinder by a cap for transport, removing the cap, introducing a filter device and concentrating the cellular material on a filter surface, after which the fluid level is at that part of the base part below the connection to the cylinder, disposing of the filtrate, removing the base part, pressing the filter surface onto an object carrier for a cell analysis, and closing the base part by the cap for further transport. 16.-. (canceled)7. A device for transportation and the preparation of cell material in a liquid for diagnostic purposes , comprising:a cylinder;a liquid-tight twist cap configured to close a first end of the cylinder with a liquid-tight seal; anda removable twist bottom part to connect to a second end of the cylinder to form a liquid tight seal;wherein the twist cap is further configured to connect to the bottom part by screwing to form a liquid-tight seal.8. The device according claim 7 , further comprising:a filter device comprising:a tube to radially fit into the cylinder, the tube having a first, open end and a second open end; anda filter closing the second open end of the tube, the filter at least partly sticking out of the tube.9. The device according claim 8 , wherein an axial depth of penetration and a geometry of the second open end of the filter device closed by the filter is configured such that after the insertion of the filter device claim 8 , the level of the liquid and the cell material in the bottom part of the cylinder is below the connection of the bottom part the cylinder.10. The device ...

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Номер записи: 98
08-08-2013 дата публикации

INFORMATION NOTIFICATION SAMPLE PROCESSING SYSTEM AND METHODS OF BIOLOGICAL SLIDE PROCESSING

Номер: US20130203103A1
Автор: Favuzzi John, Feingold Gordon, Key Marc, Welcher Rosanne
Принадлежит: DAKO DENMARK A/S

A sample processing system that may be automated and methods are disclosed where samples are arranged on a carrier element and a process operation control system automatically processes the samples perhaps robotically with an operationally-influential exteriorly-consequential information monitor or a data capture element. Significant process details as well as operationally-influential exteriorly-consequential information may be monitored and an automatic notice element may cause notification of a person at some display that may be remote. Various people may be notified, such as an administrator, a supplier, or a manufacturer of an opportunity for some action such as reagent reordering or the like. A simulated motion display may be included to “watch” simulated operation in real time or long after completion of the actual processing. 1. A method of automated sample processing comprising the steps of:establishing a network including at least one stainer, the at least one stainer having an automated process operation capability that causes automated process operation events through movement of a robotic member;receiving a protocol in the at least one stainer via the network;scheduling a plurality of sample process operations based on the protocol;monitoring operationally-influential exteriorly-consequential information;automatically processing at least one sample arranged on a first slide using the at least one stainer at least in part through operation of said robotic member sequencing through said scheduled plurality of sample process operations, wherein automatically processing includes dispensing of a buffering agent by the robotic member;inserting a second slide into the at least one stainer, wherein inserting the second slide does not interfere with movement of the robotic member related to the first slide; andcommunicating via the network at least some exteriorly-consequential information in response to said step of monitoring operationally-influential ...

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Номер записи: 99
08-08-2013 дата публикации

METHOD FOR PRE-TREATING SPECIMEN AND METHOD FOR ANALYZING SPECIMEN

Номер: US20130203105A1
Автор: Ban Kazuhiro, Hashimoto Hiroyuki, Komatsu Manabu, Murayama Yohei
Принадлежит: CANON KABUSHIKI KAISHA

A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method. 110-. (canceled)11. A treatment method for a specimen composed of a biological tissue for analyzing the specimen using at least one of an ion , an X-ray , an ultraviolet , a visible light , an infrared , and a terahertz light , the method comprising the steps of:preparing a specimen to be analyzed;determining a target portion in the specimen; andapplying an analysis inhibitor to a portion other than the target portion of the specimen so as not to apply the analysis inhibitor to the specified target portion using a droplet spray method,wherein the analysis inhibitor contains at least one of an ionization inhibitor, an X-ray absorber, an ultraviolet absorber, a visible light absorber, an infrared absorber, and a terahertz light absorber.12. The treatment method according to claim 11 , wherein the target portion is composed of a specified cell in a biological tissue section.13. The treatment method according to claim 11 , wherein the ionization inhibitor contains a nonvolatile compound containing at least one of lithium claim 11 , sodium claim 11 , potassium claim 11 , and calcium.14. The treatment method according to claim 11 , wherein the X-ray absorber contains an element with an atomic number of 40 or larger.15. The treatment method according to claim 11 , wherein one of the ultraviolet absorber claim 11 , the visible light absorber claim 11 , the infrared absorber claim 11 , and the terahertz light absorber contains a polymer compound.16. The treatment method according to claim 11 , further comprising applying an analytical reagent to the target portion.17. A method of making a treated specimen for analyzing a specimen using at least ...

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Номер записи: 100